Polygonatum sibiricum dietary fiber composite preparation and application thereof

Abstract

The invention discloses a rhizoma polygonati dietary fiber composite preparation and application thereof, and belongs to the field of food. The invention provides a polygonatum sibiricum dietary fiber composite preparation in order to solve the problems that in the prior art, the dietary fiber utilization rate is low, and preparations capable of effectively promoting intestinal tract movement and maintaining intestinal tract functions are lacked. The compound preparation is prepared from the following components in percentage by weight: 15 to 40 percent of rhizoma polygonati soluble dietary fiber, 10 to 35 percent of rhizoma polygonati insoluble dietary fiber, 2 to 4 percent of erythritol, 0.1 to 0.5 percent of stevioside and 1 to 5 percent of isomaltulose. The polygonatum sibiricum dietary fiber composite preparation provided by the invention can promote intestinal peristalsis and maintain intestinal functions, and has a certain intestinal protection function.

Description

Technical Field

[0001] The invention belongs to the field of food, and particularly relates to a polygonatum dietary fiber composite preparation and application thereof. Background Art

[0002] Polygonatum sibiricum is a perennial herbaceous plant of the genus Polygonatum in the Liliaceae family. Its fleshy, nodular rhizome is the primary part of its medicinal uses. The rhizome contains polysaccharides, glycans, flavonoids, lignin, alkaloids, and amino acids. Modern pharmacological research has shown that Polygonatum sibiricum has kidney-tonifying and essence-benefiting properties, nourishing yin and moistening dryness, providing antioxidant and anti-aging benefits, regulating immunity, lowering lipids and blood sugar, inhibiting cholesterol, preventing atherosclerosis, improving learning and memory, and exhibiting anti-inflammatory and antiviral properties. However, raw Polygonatum sibiricum is irritating to the throat and skin, and can cause numbness in the mouth and tongue. While steaming and cooking Polygonatum enhances its health benefits, it also produces a stronger sour, bitter, and medicinal flavor, resulting in a strong, sticky sensation. This negatively impacts the expansion of Polygonatum sibiricum products, leading to poor consumer acceptance.

[0003] Dietary fiber is a polysaccharide that cannot be digested and absorbed by the gastrointestinal tract, nor can it produce energy. Therefore, it was once considered a "non-nutritious substance" and long received insufficient attention. However, with the in-depth development of nutrition and related sciences, it has been discovered that the insoluble components of dietary fiber can act on the intestines to produce mechanical peristaltic effects, while the soluble components play more metabolic functions, such as affecting carbohydrate and lipid metabolism. Therefore, those skilled in the art are eager to develop a formulation based on dietary fiber that promotes the rapid reproduction and biochemical activity of probiotics in the intestines, increases intestinal peristalsis, and maintains intestinal health. Summary of the Invention

[0004] The present invention aims to solve the problems in the prior art of low dietary fiber utilization and lack of preparations that effectively promote intestinal peristalsis and maintain intestinal function, and provides a polygonatum dietary fiber composite preparation and application thereof.

[0005] One of the purposes of the present invention is to provide a polygonatum dietary fiber composite preparation, which is composed of: 15-40% polygonatum soluble dietary fiber, 10-35% polygonatum insoluble dietary fiber, 2-4% erythritol, 0.1-0.5% steviol glycosides, and 1-5% isomaltulose.

[0006] In a preferred embodiment of the present invention, the preparation method of the polygonatum dietary fiber composite preparation is: S1: Wash the polygonatum sibiricum residue, dry it at 55°C, crush it, and pass it through an 80-mesh sieve to obtain polygonatum sibiricum powder; S2: adding the polygonatum powder obtained in S1 to distilled water, adjusting the pH to 6, and adding α-amylase for primary enzymatic hydrolysis; adjusting the pH to 7, and adding papain for secondary enzymatic hydrolysis to obtain a polygonatum extract; S3: The polygonatum sibiricum extract obtained in S2 is filtered, and the filtered residue is dried to obtain polygonatum sibiricum insoluble dietary fiber; the filtered filtrate is rotary evaporated and then precipitated with 4 times the volume of ethanol for 12 hours, and the precipitate is freeze-dried to obtain polygonatum soluble dietary fiber; S4: The insoluble dietary fiber of polygonatum sibiricum and the soluble dietary fiber of polygonatum sibiricum obtained in S3 are mixed evenly in a certain proportion, a sweetener is added, and the mixture is stirred at a low speed for 5 minutes to obtain a polygonatum sibiricum dietary fiber composite preparation.

[0007] In a preferred embodiment of the present invention, the mixing volume ratio of the polygonatum powder and distilled water in S2 is 1:25.

[0008] In a preferred embodiment of the present invention, the conditions for the one-time enzymatic hydrolysis in S2 are: enzymatic hydrolysis at 60° C. for 2 h, followed by enzyme inactivation for 5 min after the enzymatic hydrolysis is completed; and the mass percentage of the α-amylase is 0.25%.

[0009] In a preferred embodiment of the present invention, the conditions of the secondary enzymatic hydrolysis in S2 are: enzymatic hydrolysis at 50° C. for 1 hour, and boiling to inactivate the enzyme after the enzymatic hydrolysis is completed; and the mass percentage of the papain is 0.3%.

[0010] In a preferred embodiment of the present invention, the sweetener in S4 is a compound of erythritol, steviol glycosides and isomaltulose.

[0011] The second object of the present invention is to provide the application of the above-mentioned polygonatum dietary fiber composite preparation in promoting intestinal peristalsis and maintaining intestinal function.

[0012] Beneficial effects of the present invention: The present invention provides a polygonatum dietary fiber composite preparation, comprising: 15-40% polygonatum soluble dietary fiber, 10-35% polygonatum insoluble dietary fiber, 2-4% erythritol, 0.1-0.5% steviol glycosides, and 1-5% isomaltulose. The present invention obtains polygonatum insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) by mixing polygonatum powder with distilled water, subjecting the mixture to a primary enzymatic hydrolysis with α-amylase and a secondary enzymatic hydrolysis with papain. The composite preparation is obtained by utilizing a specific compounding ratio to leverage the synergistic effect of the polygonatum insoluble dietary fiber and soluble dietary fiber.

[0013] The polygonatum dietary fiber composite preparation provided by the present invention has a certain binding ability for glucose, which helps to lower blood sugar levels; it has good adsorption capacity for cholesterol and can better play a role in the intestinal environment; through effect experiments, it is proved that the specific compound ratio of polygonatum insoluble dietary fiber and soluble dietary fiber has a certain promoting effect on bifidobacteria and lactic acid bacteria; specifically: higher soluble dietary fiber promotes the growth of lactic acid bacteria and the rapid fermentation of intestinal flora, generating more SCFAs; lower IDF reduces the proportion of fermentable substrates, thereby reducing the accumulation of acidic metabolites; higher IDF is not conducive to the growth of lactic acid bacteria, affecting their adhesion ability or metabolic activity; an appropriate amount of IDF can provide them with a good growth environment; therefore, the polygonatum dietary fiber composite preparation provided by the present invention has the ability to promote intestinal peristalsis, maintain intestinal function, and has a certain intestinal protection function. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The figure shows the extraction rate optimization result of Polygonatum sibiricum extract; SDF is the Polygonatum soluble dietary fiber treatment group, and IDF is the Polygonatum sibiricum insoluble dietary fiber treatment group; Figure 2 This is the result diagram of α-amylase concentration optimization; Figure 3 This is the result diagram of α-amylase enzymatic hydrolysis time optimization; Figure 4 This is the result graph of papain concentration optimization; Figure 5 This is the result diagram of papain enzymatic hydrolysis time optimization; Figure 6 The figure shows the test results of the physical properties of soluble dietary fiber and insoluble dietary fiber; among them, Water-swelling ability is the water-swelling ability, Water-retaining ability is the water-retaining ability, Oil adsorption capacity is the oil absorption capacity; the vertical axis Capacity is the capacity; Figure 7 This is a statistical chart of the adsorption capacity of soluble dietary fiber and insoluble dietary fiber for glucose; Figure 8 It is a statistical chart of the inhibition ability of soluble dietary fiber and insoluble dietary fiber on α-amylase; the vertical axis a-amylase inhibition ability is the α-amylase inhibition rate; Figure 9 This is a statistical chart of the cholesterol adsorption capacity of soluble dietary fiber and insoluble dietary fiber; Figure 10It is a statistical chart of the inhibition ability of soluble dietary fiber and insoluble dietary fiber on lipase; the vertical axis Lipase inhibition is the lipase inhibition rate; Figure 11 This is a graph showing the effects of different ratios of Polygonatum dietary fiber compound preparations on Bifidobacterium and Lactobacillus; Bifidobacterium is Bifidobacterium, and Lactobacillus is Lactobacillus; Figure 12 The figure shows the effects of different compounding ratios of Polygonatum sibiricum dietary fiber compound preparations on Enterococcus and Enterobacteriaceae; Enterococcus refers to Enterococcus, and Enterobacteriaceae refers to Enterobacteriaceae; Figure 13 This is a graph showing the effects of different ratios of Polygonatum sibiricum dietary fiber compound preparations on the pH of in vitro fermentation broth. DETAILED DESCRIPTION

[0015] Those skilled in the art can refer to the content of this document and appropriately improve the process parameters. It is particularly important to note that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in the present invention. The methods and applications of the present invention have been described through preferred embodiments. It is obvious that relevant persons can modify or appropriately change and combine the methods and applications described herein without departing from the content and scope of the present invention to implement and apply the technology of the present invention.

[0016] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below in conjunction with specific embodiments. The experimental methods used in the following examples are all conventional methods unless otherwise specified, and the materials, reagents, methods and instruments used are all conventional materials, reagents, methods and instruments in the art unless otherwise specified, and can be obtained from commercial channels by those skilled in the art.

[0017] Example 1: The composition of the polygonatum dietary fiber composite preparation described in this embodiment is: 10% polygonatum insoluble dietary fiber, 40% polygonatum soluble dietary fiber, 4% erythritol, 0.5% steviol glycoside, and 5% isomaltulose; the ratio of the polygonatum insoluble dietary fiber to the polygonatum soluble dietary fiber is 2:8.

[0018] The preparation method of the polygonatum dietary fiber composite preparation is: S1: The Polygonatum sibiricum residue was washed and dried with hot air at 55°C for 8 h, crushed, and passed through an 80-mesh sieve to obtain Polygonatum sibiricum powder; S2: The polygonatum powder obtained in S1 was added to distilled water at a mixing volume ratio of 1:25, the pH was adjusted to 6.0, and 0.25% α-amylase was added for a primary enzymatic hydrolysis. The conditions for the primary enzymatic hydrolysis were: enzymatic hydrolysis at 60°C for 2 h, and after the enzymatic hydrolysis was completed, the enzyme was boiled for 5 min to inactivate the enzyme. The pH was adjusted to 7.0, and 0.3% papain was added for a secondary enzymatic hydrolysis. The conditions for the secondary enzymatic hydrolysis were: enzymatic hydrolysis at 50°C for 1 h, and after the enzymatic hydrolysis was completed, the enzyme was boiled to inactivate the enzyme, thereby obtaining a polygonatum extract. S3: The polygonatum sibiricum extract obtained in S2 is filtered, and the filter residue is dried to obtain polygonatum sibiricum insoluble dietary fiber; the filtrate is rotary evaporated, and the filtrate: ethanol = 1:4 ratio is fully mixed, and the mixture is precipitated for 12 hours, and the precipitate is freeze-dried to obtain polygonatum soluble dietary fiber; S4: After evenly mixing 7.2 g of the insoluble dietary fiber from polygonatum sibiricum and 1.8 g of the soluble dietary fiber from polygonatum sibiricum obtained in S3, add 0.4 g of erythritol, 0.05 g of stevioside, and 0.5 g of isomaltulose, and stir at low speed for 5 min to obtain a polygonatum sibiricum dietary fiber composite preparation; divide the mixture into portions, vacuum seal, and store below 4°C.

[0019] Example 2: The difference between this embodiment and Example 1 is that the composition of the polygonatum dietary fiber composite preparation is: 25% polygonatum insoluble dietary fiber, 25% polygonatum soluble dietary fiber, 4% erythritol, 0.5% steviol glycoside, and 5% isomaltulose; the ratio of the polygonatum insoluble dietary fiber: polygonatum soluble dietary fiber is 5:5; the other parts are the same as Example 1.

[0020] Example 3: The difference between this embodiment and Example 1 is that the composition of the polygonatum dietary fiber composite preparation is: 35% polygonatum insoluble dietary fiber, 15% polygonatum soluble dietary fiber, 4% erythritol, 0.5% steviol glycoside, and 5% isomaltulose; the ratio of the polygonatum insoluble dietary fiber: polygonatum soluble dietary fiber is 7:3; the other parts are the same as Example 1.

[0021] Example 4: The difference between this embodiment and Example 1 is that the composition of the polygonatum dietary fiber composite preparation is: 15% polygonatum insoluble dietary fiber, 35% polygonatum soluble dietary fiber, 4% erythritol, 0.5% steviol glycoside, and 5% isomaltulose; the ratio of the polygonatum insoluble dietary fiber: polygonatum soluble dietary fiber is 3:7; the other parts are the same as Example 1.

[0022] Example 5: The difference between this embodiment and Example 1 is that the composition of the polygonatum dietary fiber composite preparation is: 20% polygonatum insoluble dietary fiber, 30% polygonatum soluble dietary fiber, 4% erythritol, 0.5% steviol glycoside, and 5% isomaltulose; the ratio of the polygonatum insoluble dietary fiber: polygonatum soluble dietary fiber is 4:6; the other parts are the same as Example 1.

[0023] Example 6: The difference between this embodiment and Example 1 is that the composition of the polygonatum dietary fiber composite preparation is: 30% polygonatum insoluble dietary fiber, 20% polygonatum soluble dietary fiber, 4% erythritol, 0.5% steviol glycoside, and 5% isomaltulose; the ratio of the polygonatum insoluble dietary fiber: polygonatum soluble dietary fiber is 6:4; the other parts are the same as Example 1.

[0024] Comparative Example 1: The difference between this comparative example and Example 1 is that the composition of the polygonatum dietary fiber composite preparation is: 45% polygonatum insoluble dietary fiber, 5% polygonatum soluble dietary fiber, 4% erythritol, 0.5% steviol glycoside, and 5% isomaltulose; the ratio of the polygonatum insoluble dietary fiber: polygonatum soluble dietary fiber is 9:1, and the other parts are the same as in Example 1.

[0025] Comparative Example 2: The difference between this comparative example and Example 1 is that the composition of the polygonatum dietary fiber composite preparation is: 40% polygonatum insoluble dietary fiber, 10% polygonatum soluble dietary fiber, 4% erythritol, 0.5% steviol glycoside, and 5% isomaltulose; the ratio of the polygonatum insoluble dietary fiber: polygonatum soluble dietary fiber is 8:2, and the other parts are the same as Example 1.

[0026] Comparative Example 3: The difference between this comparative example and Example 1 is that the composition of the polygonatum dietary fiber composite preparation is: 5% polygonatum insoluble dietary fiber, 45% polygonatum soluble dietary fiber, 4% erythritol, 0.5% steviol glycoside, and 5% isomaltulose; the ratio of the polygonatum insoluble dietary fiber: polygonatum soluble dietary fiber is 1:9, and the other parts are the same as in Example 1.

[0027] Effect experiment: 1. Optimization of the preparation process of Polygonatum sibiricum dietary fiber compound preparation (1) Optimization of the liquid-to-material ratio of Polygonatum sibiricum powder and distilled water: The polygonatum sibiricum residue was washed, dried at 55°C, crushed, and passed through an 80-mesh sieve to obtain polygonatum sibiricum powder; the polygonatum sibiricum powder was added to distilled water at a mixing volume ratio of 1:15, 1:20, 1:25, 1:30, and 1:35, respectively, and the pH was adjusted to 6.0. 0.25% α-amylase was added for a primary enzymatic hydrolysis. The conditions for the primary enzymatic hydrolysis were: enzymatic hydrolysis at 60°C for 2 h, and the enzyme was inactivated for 5 min after the enzymatic hydrolysis was completed; the pH was adjusted to 7.0, 0.3% papain was added for a secondary enzymatic hydrolysis. The conditions for the secondary enzymatic hydrolysis were: enzymatic hydrolysis at 50°C for 1 h, and the enzyme was boiled and inactivated after the enzymatic hydrolysis was completed to obtain the polygonatum sibiricum extract.

[0028] The present invention tests the extraction rate of the polygonatum extract obtained by the above method, and the results are as follows Figure 1 As shown in the figure, when the material-liquid ratio increases from 1:15 to 1:25, the dietary fiber extraction rate gradually increases; when the material-liquid ratio continues to increase, the dietary fiber extraction rate decreases. Therefore, 1:25 is selected as the optimal liquid-to-material ratio for the mixture of polygonatum powder and distilled water.

[0029] (2) Optimization of α-amylase concentration and enzymatic hydrolysis time: The polygonatum sibiricum residue was washed, dried at 55°C, crushed, and passed through an 80-mesh sieve to obtain polygonatum sibiricum powder; the polygonatum sibiricum powder was added to distilled water at a mixing volume ratio of 1:25, and the pH was adjusted to 6.0. 0.15%, 0.20%, 0.25%, 0.30% and 0.35% α-amylase were added respectively for primary enzymatic hydrolysis. The conditions for the primary enzymatic hydrolysis were: enzymatic hydrolysis at 60°C for 1.0, 1.5, 2.0, 2.5 and 3.0 h, respectively, and the enzyme was inactivated for 5 min after the enzymatic hydrolysis was completed; the pH was adjusted to 7.0, 0.3% papain was added for secondary enzymatic hydrolysis. The conditions for the secondary enzymatic hydrolysis were: enzymatic hydrolysis at 50°C for 1 h, and the enzyme was boiled and inactivated after the enzymatic hydrolysis was completed to obtain the polygonatum sibiricum extract.

[0030] The present invention tests the extraction rate of the polygonatum extract obtained by the above method, and the results are as follows Figure 2-3 As shown in the figure, when the α-amylase concentration increased from 0.15% to 0.25%, the dietary fiber extraction rate gradually increased; when the α-amylase concentration continued to increase, the dietary fiber extraction rate decreased; when the α-amylase enzymatic hydrolysis time increased from 1 h to 2 h, the dietary fiber extraction rate gradually increased; when the α-amylase enzymatic hydrolysis time continued to increase, the dietary fiber extraction rate decreased. Therefore, the α-amylase concentration of 0.25% and the α-amylase enzymatic hydrolysis time of 2 h were selected.

[0031] (3) Optimization of papain concentration and enzymatic hydrolysis time: The polygonatum sibiricum residue was washed, dried at 55°C, crushed, and passed through an 80-mesh sieve to obtain polygonatum sibiricum powder; the polygonatum sibiricum powder was added to distilled water at a mixing volume ratio of 1:25, the pH was adjusted to 6.0, and 0.25% α-amylase was added for primary enzymatic hydrolysis. The conditions for the primary enzymatic hydrolysis were: enzymatic hydrolysis at 60°C for 2.0 h, and boiling and inactivating the enzyme after the enzymatic hydrolysis was completed for 5 min; the pH was adjusted to 7.0, and 0.15%, 0.20%, 0.25%, 0.30% and 0.35% papain were added for secondary enzymatic hydrolysis, respectively. The conditions for the secondary enzymatic hydrolysis were: enzymatic hydrolysis at 50°C for 0.5, 1.0, 1.5, 2.0 and 2.5 h, respectively, and boiling and inactivating the enzyme after the enzymatic hydrolysis was completed to obtain the polygonatum sibiricum extract.

[0032] The present invention tests the extraction rate of the polygonatum extract obtained by the above method, and the results are as follows Figure 4-5 As shown in the figure, when the papain concentration increased from 0.15% to 0.3%, the dietary fiber extraction rate gradually increased; when the papain concentration continued to increase, the dietary fiber extraction rate decreased; when the papain enzymatic hydrolysis time increased from 1 h to 2 h, the dietary fiber extraction rate gradually increased; when the papain enzymatic hydrolysis time continued to increase, the dietary fiber extraction rate decreased. Therefore, a papain concentration of 0.3% and a papain enzymatic hydrolysis time of 1 h were selected.

[0033] 2. Physical properties of soluble and insoluble dietary fiber The polygonatum sibiricum residue was washed, dried at 55°C, crushed, and passed through an 80-mesh sieve to obtain polygonatum sibiricum powder; the polygonatum sibiricum powder was added to distilled water at a mixing volume ratio of 1:25, the pH was adjusted to 6.0, and 0.25% α-amylase was added for primary enzymatic hydrolysis. The conditions for the primary enzymatic hydrolysis were: enzymatic hydrolysis at 60°C for 2.0 h, and boiling and inactivating the enzyme after the enzymatic hydrolysis was completed for 5 min; the pH was adjusted to 7.0, and 0.30% papain was added for secondary enzymatic hydrolysis. The conditions for the secondary enzymatic hydrolysis were: enzymatic hydrolysis at 50°C for 1.0 h, and boiling and inactivating the enzyme after the enzymatic hydrolysis was completed to obtain polygonatum sibiricum extract; the polygonatum sibiricum extract was filtered, and the filtered residue was dried to obtain polygonatum sibiricum insoluble dietary fiber; the filtrate was rotary evaporated, mixed with filtrate: ethanol at a ratio of 1:4, precipitated for 12 h, and the precipitate was freeze-dried to obtain polygonatum soluble dietary fiber.

[0034] The present invention tests the water holding capacity, swelling capacity and oil holding capacity of the soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) obtained by the above method, specifically: Water holding capacity determination: Accurately weigh 0.5 g of the sample to be tested and place it in a 50 mL centrifuge tube. Add 50 mL of distilled water, mix well, and centrifuge at 6500 r / min for 30 min at 4°C. Discard the supernatant and weigh the precipitate of the sample to be tested.

[0035] Expansion force determination: Take 0.5 g of the sample to be tested, place it in a 10 mL graduated cylinder, record the volume, add 5 mL of distilled water, shake evenly, and place it at 25°C for 24 h. Record the volume of the sample to be tested at this time.

[0036] Oil holding capacity determination: Accurately weigh 2 g of the sample to be tested and place it in a 100 mL beaker. Add 20 mL of vegetable oil and shake to mix every 5 minutes within 30 minutes. Centrifuge at 6500 r / min, 15°C for 30 minutes, discard the soybean oil on the top layer, and then measure the mass of the sample.

[0037] The results are as follows Figure 6 As shown, the soluble dietary fiber (SDF) of Polygonatum sibiricum has high water absorption and swelling properties and good water retention, while the soluble dietary fiber (IDF) exhibits stronger oil absorption capacity. The present invention utilizes the synergistic effect between SDF and IDF to give full play to their advantages in physical functional properties, thereby achieving the effect of regulating the intestine and controlling blood lipids.

[0038] 3. In vitro hypoglycemic effects of soluble and insoluble dietary fiber The polygonatum sibiricum residue was washed, dried at 55°C, crushed, and passed through an 80-mesh sieve to obtain polygonatum sibiricum powder; the polygonatum sibiricum powder was added to distilled water at a mixing volume ratio of 1:25, the pH was adjusted to 6.0, and 0.25% α-amylase was added for primary enzymatic hydrolysis. The conditions for the primary enzymatic hydrolysis were: enzymatic hydrolysis at 60°C for 2.0 h, and boiling and inactivating the enzyme after the enzymatic hydrolysis was completed for 5 min; the pH was adjusted to 7.0, and 0.30% papain was added for secondary enzymatic hydrolysis. The conditions for the secondary enzymatic hydrolysis were: enzymatic hydrolysis at 50°C for 1.0 h, and boiling and inactivating the enzyme after the enzymatic hydrolysis was completed to obtain polygonatum sibiricum extract; the polygonatum sibiricum extract was filtered, and the filtered residue was dried to obtain polygonatum sibiricum insoluble dietary fiber; the filtrate was rotary evaporated, and the filtrate: ethanol = 1:4 was fully mixed, precipitated for 12 h, and the precipitate was freeze-dried to obtain polygonatum soluble dietary fiber.

[0039] The present invention tests the in vitro hypoglycemic effect of the soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) obtained by the above method, specifically: Determination of glucose adsorption capacity: Accurately weigh 0.25 g of the sample to be tested and mix it with 25 mL of 50 mmol / L and 100 mmol / L glucose solutions, respectively. After the mixture was shaken in a constant temperature water bath shaker at 37°C for 6 h, it was centrifuged at 3500 rpm for 15 min. The supernatant was collected and the glucose concentration in the supernatant was determined by the DNS method.

[0040] α-Amylase Inhibition Assay: Add 0.25 g of the test sample and 1 mg of pancreatic α-amylase (40 U / mg) to a test tube. Add 10 mL of potato starch solution (4%, 0.05 M phosphate buffer, pH 6.5) to this mixture and mix. Place the mixture in a 37°C water bath for 1 hour. Add 20 mL of 0.1 M NaOH to the mixture and centrifuge at 6000 rpm for 10 minutes. Determine the glucose content in the supernatant using the DNS method. Prepare a control solution without dietary fiber and a blank solution without substrate.

[0041] The results are as follows Figure 7-8 As shown in the results, under the treatment of glucose concentrations of 50 mmol / L and 100 mmol / L, both SDF and IDF have the ability to adsorb glucose; the glucose adsorption capacity of SDF is significantly higher than that of IDF, and the adsorption amount increases with the increase of glucose concentration; and SDF has a stronger inhibitory ability on α-amylase, indicating that both SDF and IDF have a certain binding ability for glucose, which helps to lower blood sugar levels.

[0042] 4. In vitro lipid-lowering effects of soluble and insoluble dietary fiber The polygonatum sibiricum residue was washed, dried at 55°C, crushed, and passed through an 80-mesh sieve to obtain polygonatum sibiricum powder; the polygonatum sibiricum powder was added to distilled water at a mixing volume ratio of 1:25, the pH was adjusted to 60.0, and 0.25% α-amylase was added for primary enzymatic hydrolysis. The conditions for the primary enzymatic hydrolysis were: enzymatic hydrolysis at 60°C for 2 h, and boiling and inactivating the enzyme after the enzymatic hydrolysis was completed for 5 min; the pH was adjusted to 70.0, and 0.30% papain was added for secondary enzymatic hydrolysis. The conditions for the secondary enzymatic hydrolysis were: enzymatic hydrolysis at 50°C for 1 h, and boiling and inactivating the enzyme after the enzymatic hydrolysis was completed to obtain polygonatum sibiricum extract; the polygonatum sibiricum extract was filtered, and the filtered residue was dried to obtain polygonatum sibiricum insoluble dietary fiber; the filtrate was rotary evaporated, and the filtrate: ethanol = 1:4 ratio was fully mixed, precipitated for 12 h, and the precipitate was freeze-dried to obtain polygonatum soluble dietary fiber.

[0043] The present invention performs in vitro lipid-lowering effects on the soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) of Polygonatum obtained by the above method, specifically: Determination of cholesterol adsorption capacity: Deionized water and fresh egg yolk were thoroughly mixed in a 9:1 ratio. 0.5 g of the sample to be tested was added to a 100 mL Erlenmeyer flask. The above 25 g diluted egg yolk solution was added and the pH of the solution was adjusted to 2.0 and 7.0 respectively after thorough mixing (the adsorption capacity for cholesterol was different at different pH values). The solution was shaken at 37°C for 2 h and centrifuged at 4000 rpm for 20 min. 290 μL of the supernatant was aspirated and 0.4 mL of glacial acetic acid, 1.5 mL of o-phthalaldehyde solution and 1 mL of H2SO4 solution were added respectively. After thorough mixing, the solution was allowed to stand at room temperature for 10 min. The absorbance of the sample to be tested was measured at 550 nm using a spectrophotometer.

[0044] Lipase inhibition ability assay: Add 2 mL of tris buffer (pH = 8.2, 100 mM) to 25 mg of the sample to be tested and vortex for 1 min; add 1 mL of lipase enzyme solution (2 mg / mL prepared in distilled water) and 1 mL of p-nitrophenyl palmitate substrate solution (0.015% prepared in dimethyl sulfoxide) and vortex for 1 min; prepare a control solution without dietary fiber and a blank solution without dietary fiber and lipase; place the mixture in a constant temperature water bath shaker, incubate at 37°C and 120 rpm for 30 min, react in a boiling water bath for 10 min, return to room temperature, and centrifuge at 8000 rpm for 10 min. The absorbance of the sample to be tested is measured at 400 nm using a spectrophotometer.

[0045] The results are as follows Figure 9-10 As shown, both SDF and IDF were able to adsorb cholesterol at both pH 2.0 and pH 7.0. The best cholesterol adsorption capacity was achieved at pH 2.0, indicating that both SDF and IDF functioned better in the intestinal environment. Furthermore, SDF exhibited a stronger lipase inhibitory effect, suggesting that SDF has a certain inhibitory effect on lipase and can help lower cholesterol levels.

[0046] 5. Effects of different ratios of Polygonatum sibiricum dietary fiber compound preparations on important intestinal bacteria The polygonatum dietary fiber composite preparations prepared in Examples 1-6 and Comparative Examples 1-3 were tested for the effects of bifidobacteria and lactic acid bacteria.

[0047] The results are as follows Figure 11 As shown in the figure, when the mixing ratio of insoluble dietary fiber (IDF) of Polygonatum sibiricum and soluble dietary fiber (SDF) of Polygonatum sibiricum is (2-7): (3-8), the prepared composite preparation has a certain promoting effect on the proliferation of bifidobacteria and lactic acid bacteria.

[0048] In the high SDF (mixing ratio of 1:9 and 2:8) treatment groups, the number of bifidobacteria and lactic acid bacteria increased significantly, especially the number of lactic acid bacteria reached the highest in the treatment group with a mixing ratio of 2:8; this shows that higher soluble dietary fiber (SDF) in Polygonatum sibiricum promoted the growth of lactic acid bacteria. The reason is that SDF can serve as a substrate for probiotics, thereby increasing the metabolic activity of the bacteria.

[0049] Among the treatment groups with moderate IDF (mixing ratios of 3:7, 4:6, 5:5 and 6:4), the number of bifidobacteria reached the highest in the treatment group with a mixing ratio of 5:5, indicating that an appropriate amount of IDF promoted the growth of bifidobacteria; the number of lactic acid bacteria also had a high value in the treatment group with a mixing ratio of 3:7, but then decreased. The reason is that the gradual decrease in SDF led to the restriction of lactic acid bacteria metabolism.

[0050] In the high IDF (mixing ratio of 7:3, 8:2 and 9:1) treatment groups, the number of bifidobacteria decreased in the mixing ratio of 8:2 and 9:1 treatment groups, indicating that excessive IDF would reduce the growth advantage of bifidobacteria; the number of lactic acid bacteria decreased significantly in the mixing ratio of 8:2 and 9:1 treatment groups, especially reaching the lowest in the mixing ratio of 9:1 treatment group, indicating that high IDF may be detrimental to the growth of lactic acid bacteria and affect their normal growth and metabolic activities.

[0051] The results are as follows Figure 12 As shown in the figure, when the mixing ratio of Polygonatum sibiricum insoluble dietary fiber (IDF) and Polygonatum sibiricum soluble dietary fiber (SDF) is 2:8 and 7:3, the prepared composite preparation has a certain inhibitory effect on enterococci and enterobacteriaceae.

[0052] In the high SDF (mixing ratio of 1:9 and 2:8) treatment groups, the colony counts of enterococci and enterobacteriaceae decreased, especially in the treatment group with a mixing ratio of 2:8, where the colony counts decreased most significantly; this suggests that high SDF may promote the growth of certain probiotics (such as lactic acid bacteria and bifidobacteria), thereby inhibiting the growth of enterococci and enterobacteriaceae.

[0053] Among the treatment groups with moderate IDF (mixing ratios of 3:7, 4:6, 5:5 and 6:4), the number of enterococcal colonies reached the highest in the treatment group with a mixing ratio of 3:7, indicating that an appropriate amount of IDF promoted the proliferation of enterococci; the number of Enterobacteriaceae slightly rebounded in the treatment group with a mixing ratio of 5:5, indicating that this ratio may be conducive to the growth of certain opportunistic pathogens, which may be related to the pH buffering effect.

[0054] Among the high IDF treatments (mixing ratios of 7:3, 8:2, and 9:1), Enterococcus abundance was lowest in the 7:3 treatment and rapidly recovered in the 8:2 and 9:1 treatments, suggesting that high IDF may suppress Enterococci to some extent, but beyond a certain threshold, their more adaptable populations will re-grow. Enterobacteriaceae abundance was also lowest in the 7:3 treatment and remained stable in the 8:2 and 9:1 treatments, suggesting that high IDF may reduce their survival advantage, but the effect was less significant than that of SDF.

[0055] 6. Effects of different ratios of Polygonatum sibiricum dietary fiber composite preparations on the pH of in vitro fermentation broth The in vitro fermentation broth pH of the Polygonatum sibiricum dietary fiber composite preparations prepared in Examples 1-6 and Comparative Examples 1-3 was tested.

[0056] The results are as follows Figure 13 As shown in the results, when the ratio of Polygonatum soluble dietary fiber (SDF) to Polygonatum insoluble dietary fiber (IDF) was 2:8 and 7:3, the pH of the prepared composite preparation decreased the most. This is because the higher soluble dietary fiber (SDF) promoted the rapid fermentation of intestinal flora, producing more SCFAs, such as acetic acid, propionic acid, and butyric acid. When the mixing ratio was 1:9 and 2:8, the pH of the prepared composite preparation decreased less, indicating that the increase in IDF reduced the proportion of fermentable substrates, thereby reducing the accumulation of acidic metabolites.

[0057] The contents not described in detail in the present specification are well-known technologies to those skilled in the art. Although the present invention has been disclosed above with reference to preferred embodiments, they are not intended to limit the present invention. Anyone skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be defined by the claims.

Claims

1. A dietary fiber composite preparation of polygonatum, characterized in that The composite preparation is composed of: 15-40% of polygonatum soluble dietary fiber, 10-35% of polygonatum insoluble dietary fiber, 2-4% erythritol, 0.1-0.5% stevioside, and 1-5% isomaltulose.

2. The polygonatum dietary fiber composite preparation according to claim 1, characterized in that The preparation method of the polygonatum dietary fiber composite preparation is: S1: Wash the polygonatum sibiricum residue, dry it at 55°C, crush it, and pass it through an 80-mesh sieve to obtain polygonatum sibiricum powder; S2: adding the polygonatum powder obtained in S1 to distilled water, adjusting the pH to 6, and adding α-amylase for primary enzymatic hydrolysis; adjusting the pH to 7, and adding papain for secondary enzymatic hydrolysis to obtain a polygonatum extract; S3: The polygonatum sibiricum extract obtained in S2 is filtered, and the filtered residue is dried to obtain polygonatum sibiricum insoluble dietary fiber; the filtered filtrate is rotary evaporated and then precipitated with 4 times the volume of ethanol for 12 hours, and the precipitate is freeze-dried to obtain polygonatum soluble dietary fiber; S4: The insoluble dietary fiber of polygonatum sibiricum and the soluble dietary fiber of polygonatum sibiricum obtained in S3 are mixed evenly in a certain proportion, a sweetener is added, and the mixture is stirred at a low speed for 5 minutes to obtain a polygonatum sibiricum dietary fiber composite preparation.

3. The polygonatum dietary fiber composite preparation according to claim 2, characterized in that The mixing volume ratio of the polygonatum powder and distilled water in S2 is 1:

25.

4. The polygonatum dietary fiber composite preparation according to claim 2, characterized in that The conditions for one enzymatic hydrolysis in S2 are: enzymatic hydrolysis at 60° C. for 2 h, followed by enzyme inactivation for 5 min after the enzymatic hydrolysis is completed; and the mass percentage of the α-amylase is 0.25%.

5. The polygonatum dietary fiber composite preparation according to claim 2, characterized in that The conditions of the secondary enzymatic hydrolysis in S2 are: enzymatic hydrolysis at 50° C. for 1 h, and boiling to inactivate the enzyme after the enzymatic hydrolysis is completed; the mass percentage of the papain is 0.3%.

6. The polygonatum dietary fiber composite preparation according to claim 2, characterized in that The sweetener described in S4 is a compound of erythritol, steviol glycoside and isomaltulose.

7. Use of the polygonatum dietary fiber composite preparation according to any one of claims 1 to 6 in promoting intestinal motility and maintaining intestinal function.

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