Composition containing vitriol and sodium mannose phosphate and application of composition in functional skin care product

Abstract

The invention provides a composition containing glaine and sodium mannose phosphate and application of the composition in functional skin care products. The composition is prepared from the following components in percentage by mass: 12 to 25 percent of hydroxypropyl tetrahydropyrantriol, 0.05 to 1 percent of sodium mannose phosphate, 0.001 to 0.01 percent of acetyl octapeptide-3, 0.1 to 0.5 percent of saccharomycetes / rice fermentation product filtrate, 0.01 to 0.15 percent of bifidus yeast fermentation product filtrate, 0.2 to 0.5 percent of lactobacillus / soybean milk fermentation product filtrate and the balance of water or other solvents. According to the composition, through synergistic matching of all the components, the composition can resist wrinkles, whiten the skin and brighten the skin color. The composition is used in skin care products, can effectively resist wrinkles, whiten and brighten skin, and enables the skin to keep a good state.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a composition containing bosine and sodium mannose phosphate and application of the composition in functional skin care products. Background Art

[0002] As living standards improve, the demand for skin care products continues to grow. Anti-wrinkle and brightening skin tone are two of the most sought-after benefits in the skincare market. Wrinkles are closely related to factors such as collagen loss and changes in the extracellular matrix; while dull skin tone is primarily caused by excessive melanin production and accumulation.

[0003] Currently, most anti-wrinkle skincare products on the market work by promoting collagen synthesis and enhancing skin elasticity, while whitening skincare products primarily target tyrosinase inhibition and melanin metabolism to achieve their whitening effects. However, existing skincare products with single ingredients or simple formulas often suffer from limited efficacy, limited effectiveness, and slow onset, making them unable to simultaneously meet consumers' dual needs for anti-wrinkle and whitening. Therefore, developing a skincare composition that effectively fights wrinkles while also whitening and brightening the skin has significant market value and practical significance. Summary of the Invention

[0004] The object of the present invention is to provide a composition containing bosine and sodium mannose phosphate, which can achieve the dual effects of anti-wrinkle and whitening and brightening the skin tone through the synergistic ratio of the components; at the same time, the present invention also provides the use of the composition in functional skin care products to prepare products with good skin care effects.

[0005] To achieve the above object, the present invention adopts the following technical solutions: A composition containing bosacein and sodium mannose phosphate comprises the following components in percentage by mass: 12-25% hydroxypropyl tetrahydropyrantriol, 0.05-1% sodium mannose phosphate, 0.001-0.01% acetyl octapeptide-3, 0.1-0.5% yeast / rice fermentation product filtrate, 0.01-0.15% bifid yeast fermentation product filtrate, 0.2-0.5% lactobacillus / soy milk fermentation product filtrate, and the remainder solvent.

[0006] Optionally, the composition includes the following components in percentage by mass: 15-22% of the hydroxypropyl tetrahydropyrantriol, 0.1-0.8% of the sodium mannose phosphate, 0.003-0.008% of the acetyl octapeptide-3, 0.2-0.4% of the yeast / rice fermentation product filtrate, 0.03-0.12% of the bifid yeast fermentation product filtrate, 0.3-0.45% of the lactobacillus / soy milk fermentation product filtrate, and the remainder of the solvent.

[0007] Optionally, the composition includes the following components in the following mass percentages: 20% of the hydroxypropyl tetrahydropyrantriol, 0.5% of the sodium mannose phosphate, 0.005% of the acetyl octapeptide-3, 0.3% of the yeast / rice fermentation product filtrate, 0.08% of the bifid yeast fermentation product filtrate, 0.4% of the lactobacillus / soy milk fermentation product filtrate, and the remainder of the solvent.

[0008] Optionally, the solvent includes one or more of water, propylene glycol, butylene glycol and glycerol.

[0009] Optionally, the preparation method of the yeast / rice fermentation product filtrate includes: mixing rice flour and water with a solid-liquid ratio of 1:5-1:10 to obtain a first mixture; sterilizing the first mixture at 120-130°C for 20-30 minutes, cooling to 25-35°C, and then inoculating yeast, with the mass percentage of yeast in the first mixture being 1-5%, to obtain a second mixture; aerobically fermenting the second mixture at 30-32°C for 12-24 hours, and then anaerobically fermenting it at 25-28°C for 2-5 days; filtering the mixture after anaerobically fermenting, and taking the filtrate as the yeast / rice fermentation product filtrate.

[0010] Optionally, the method for preparing the bifid yeast fermentation product filtrate includes: activating the bifidobacterium strain for 2-3 generations, culturing the strain anaerobically in a culture medium including glucose, soy peptone and dipotassium hydrogen phosphate at a pH of 6.5-7.5 and a temperature of 36-38°C for 18-24 hours until the bacterial density reaches a peak, after culturing, breaking the bacterial cells at 100-150 MPa to release intracellular substances, and adding lysozyme in an amount of 0.1-0.5 mg / mL, and acting at 37-39°C for 1-2 hours to promote cell wall division, and then centrifuging the lysate to remove bacterial fragments, and passing the clear liquid through an ultrafiltration membrane to obtain the bifid yeast fermentation product filtrate.

[0011] Optionally, the mass ratio of the glucose, the soy peptone and the dipotassium hydrogen phosphate is 5-20:5-15:1-5.

[0012] Optionally, the preparation method of the lactobacillus / soy milk fermentation product filtrate includes: soaking soybeans for 8-12 hours, beating and boiling for 10-15 minutes, filtering to remove dregs, adjusting the soy milk concentration to 8-10°Bé, sterilizing the soy milk at 115-120°C for 15-20 minutes, cooling to 35-40°C, inoculating plant lactobacillus at an inoculum rate of 2-3%, and then fermenting at 37-40°C for 8-12 hours until the pH value drops to 4.0-4.5, filtering the fermentation product to remove bacteria and residues, and passing the filtrate through an ultrafiltration membrane to obtain the lactobacillus / soy milk fermentation product filtrate.

[0013] A skin care product comprises the above-mentioned composition containing bosacein and sodium mannose phosphate.

[0014] Optionally, the mass percentage of the composition containing bosine and sodium mannose phosphate in the skin care product is 0.5%-5%.

[0015] Beneficial effects In the composition of the present invention, hydroxypropyl tetrahydropyrantriol can stimulate the production of mucopolysaccharides, promoting the synthesis of extracellular matrix components such as collagen and elastin in the skin, thereby increasing skin elasticity and firmness and reducing the formation of wrinkles. Sodium mannose phosphate can inhibit the activity of tyrosinase, reducing the production of melanin, while also promoting the metabolism of keratinocytes and accelerating the excretion of melanin, achieving the effect of whitening and brightening the skin tone. Acetyl octapeptide-3 can inhibit the release of neurotransmitters, reduce muscle contraction, and thus reduce the formation of dynamic wrinkles. The yeast / rice fermentation product filtrate is rich in various nutrients and active substances, has skin-nourishing and antioxidant effects, and helps maintain the skin's health. The bifid yeast fermentation product filtrate has excellent skin repair and protection functions, can enhance the skin's resistance, and promote the repair and regeneration of skin cells. The lactobacillus / soy milk fermentation product filtrate is rich in probiotic metabolites, which can regulate the skin's microecological balance, improve the skin's condition, enhance the skin's moisturizing ability, and make the skin more hydrated and lustrous. These components work together synergistically, not only achieving the effects of anti-wrinkle and whitening and brightening skin tone, but also comprehensively improving the overall condition of the skin and keeping the skin at a good level of health. DETAILED DESCRIPTION

[0016] An embodiment of the present invention provides a composition containing bosine and sodium mannose phosphate, comprising the following components in percentage by mass: 12-25% hydroxypropyl tetrahydropyrantriol, 0.05-1% sodium mannose phosphate, 0.001-0.01% acetyl octapeptide-3, 0.1-0.5% yeast / rice fermentation product filtrate, 0.01-0.15% bifid yeast fermentation product filtrate, 0.2-0.5% lactobacillus / soy milk fermentation product filtrate, and the remainder solvent.

[0017] It can be understood that hydroxypropyl tetrahydropyrantriol is benzoin.

[0018] Furthermore, the composition comprises the following components in the following mass percentages: 15-22% hydroxypropyl tetrahydropyrantriol, 0.1-0.8% sodium mannose phosphate, 0.003-0.008% acetyl octapeptide-3, 0.2-0.4% yeast / rice fermentation product filtrate, 0.03-0.12% bifid yeast fermentation product filtrate, 0.3-0.45% lactobacillus / soymilk fermentation product filtrate, and the remainder solvent. Within this range, the synergistic effects between the ingredients can be more fully realized. For example, a more precise hydroxypropyl tetrahydropyrantriol content can more effectively stimulate collagen synthesis, and in combination with other ingredients, further enhance the anti-wrinkle effect. At the corresponding optimized content, sodium mannose phosphate, acetyl octapeptide-3, and the three fermentation product filtrates can better synergistically inhibit melanin production and regulate the skin microbiome, thereby achieving a more ideal balance and enhanced effect in terms of anti-wrinkle and whitening and brightening.

[0019] Furthermore, the composition includes the following components in the following mass percentages: 20% hydroxypropyl tetrahydropyrantriol, 0.5% sodium mannose phosphate, 0.005% acetyl octapeptide-3, 0.3% yeast / rice fermentation product filtrate, 0.08% bifida fermentation product filtrate, 0.4% lactobacillus / soymilk fermentation product filtrate, and the remainder solvent. With this ratio, the composition achieves optimal comprehensive effects in promoting collagen synthesis, inhibiting tyrosinase activity, and regulating the skin microbiome, maximizing its anti-wrinkle and skin-whitening and brightening effects. This provides a clear reference for the precise formulation design of skincare products, facilitates the production of high-quality, effective skincare products with excellent and stable effects, and satisfies consumers' demand for highly effective skincare products.

[0020] Furthermore, the solvent includes one or more of water, propylene glycol, butylene glycol and glycerol. These solvents have good solubility and safety, can effectively dissolve other active ingredients in the composition, make each component evenly dispersed, and ensure the stability and homogeneity of the composition. At the same time, propylene glycol, butylene glycol and glycerol have a moisturizing effect, can replenish moisture to the skin while dissolving the components, enhance the moisturizing properties of the skin, and cooperate with other anti-wrinkle and whitening ingredients in the composition to enhance the overall skin care effect of the skin care product. In addition, these solvents are widely available and cost-controlled, which is conducive to the large-scale application and production of the composition in skin care products.

[0021] Furthermore, the method for preparing the yeast / rice fermentation product filtrate includes: mixing rice flour and water at a solid-liquid ratio of 1:5-1:10 to obtain a first mixture; sterilizing the first mixture at 120-130°C for 20-30 minutes, cooling it to 25-35°C, and then inoculating yeast at a yeast concentration of 1-5% by weight to obtain a second mixture; aerobically fermenting the second mixture at 30-32°C for 12-24 hours, followed by anaerobically fermenting it at 25-28°C for 2-5 days; filtering the mixture after anaerobically fermenting it, and collecting the filtrate as the yeast / rice fermentation product filtrate. Through the process steps of mixing at a specific solid-liquid ratio, sterilizing, inoculating, aerobic and anaerobic fermentation, and filtration, the various beneficial components produced during the rice and yeast fermentation process, such as vitamins, amino acids, and polysaccharides, are fully extracted. These components provide rich nutrients to skin cells, promoting cell growth and repair, while also having antioxidant properties, reducing free radical damage to the skin.

[0022] Furthermore, the preparation method of the bifid yeast fermentation product filtrate includes: activating the bifidobacterium strain for 2-3 generations, culturing the strain in a culture medium including glucose, soy peptone and dipotassium hydrogen phosphate at a pH of 6.5-7.5 and a temperature of 36-38°C for 18-24 hours until the bacterial density reaches a peak, and then disrupting the bacterial cells at 100-150 MPa to release intracellular substances, and adding lysozyme at an addition amount of 0.1-0.5 mg / mL, and acting at 37-39°C for 1-2 hours to promote cell wall fission, and then centrifuging the lysate to remove bacterial fragments, and passing the supernatant through an ultrafiltration membrane to obtain the bifid yeast fermentation product filtrate. From a series of operations such as strain activation, specific culture medium cultivation, bacterial disruption, addition of lysozyme to centrifugation and ultrafiltration, a variety of active ingredients such as amino acids, minerals, and small molecule peptides produced during the bifidobacterium fermentation process can be effectively extracted. These active ingredients can protect the skin from damage by external factors, promote DNA repair, and enhance skin resistance. At the same time, the preparation method ensures the quality and activity of the filtrate of the bifid yeast fermentation product, enabling it to better cooperate with other ingredients in the composition to jointly enhance the efficacy of the composition in anti-wrinkle and whitening and brightening skin tone, and provides a standardized process for the industrial production of the bifid yeast fermentation product filtrate, which is conducive to ensuring the stability and consistency of product quality.

[0023] Furthermore, the mass ratio of glucose, soy peptone and dipotassium hydrogen phosphate is 5-20: 5-15: 1-5. The appropriate mass ratio can provide an optimal growth environment for bifidobacteria, allowing bifidobacteria to multiply in large quantities during the culture process and produce abundant beneficial metabolites.

[0024] Furthermore, a method for preparing a lactobacillus / soymilk fermentation product filtrate includes soaking soybeans for 8-12 hours, beating them, boiling them for 10-15 minutes, filtering to remove the dregs, adjusting the soymilk concentration to 8-10°Bé, sterilizing the soymilk at 115-120°C for 15-20 minutes, cooling it to 35-40°C, inoculating it with Lactobacillus plantarum at a 2-3% inoculum, and then fermenting it at 37-40°C for 8-12 hours until the pH drops to 4.0-4.5. The fermented product is filtered to remove bacteria and residue, and the filtrate is passed through an ultrafiltration membrane to obtain a lactobacillus / soymilk fermentation product filtrate. Through the steps of soaking soybeans, beating them, sterilizing, inoculating them, fermenting them, filtering, and ultrafiltration, substances such as organic acids and bacteriocins produced by lactobacilli during the soymilk fermentation process can be extracted. These substances can regulate the pH of the skin, inhibit the growth of harmful bacteria, maintain the balance of the skin's microbiome, and create a healthy environment for the skin.

[0025] Another embodiment of the present invention provides a skin care product comprising the above-mentioned composition containing bosine and sodium mannose phosphate. Compared with traditional skin care products, the skin care products of the present invention, by adding this composition, can improve skin condition from multiple aspects such as cell metabolism, pigment regulation, and skin microecological regulation, effectively reduce wrinkles, brighten skin tone, and provide consumers with more targeted and efficient skin care product options. At the same time, the addition of this composition enriches the functional ingredient system of skin care products, expands the research and development ideas and product types of functional skin care products, and helps promote the development of the skin care industry in a more efficient and professional direction.

[0026] Furthermore, the composition containing bosylamine and sodium mannose phosphate accounts for 0.5%-5% by weight of the skin care product. Within this range, the active ingredients in the composition can achieve sufficient anti-wrinkle and skin-whitening and brightening effects while also ensuring the safety and stability of the skin care product. If the composition content is too low, the desired skin care effect may not be achieved; if the content is too high, product costs may increase, and even excessive concentrations of certain ingredients may cause skin irritation and other problems.

[0027] Example 1 In this embodiment, the composition comprises the following components in the following weight percentages: 20% hydroxypropyl tetrahydropyrantriol, 0.5% sodium mannose phosphate, 0.005% acetyl octapeptide-3, 0.3% yeast / rice fermentation product filtrate, 0.08% bifida fermentation product filtrate, 0.4% lactobacillus / soymilk fermentation product filtrate, and the remainder water. The sum of the weight percentages of the components is 100%. The components are stirred and mixed to obtain the composition.

[0028] The yeast / rice fermentation product filtrate is prepared by mixing rice flour and water at a solid-to-liquid ratio of 1:8 to obtain a first mixture. The first mixture is sterilized at 125°C for 25 minutes, cooled to 30°C, and then inoculated with yeast at a concentration of 3% by weight of the first mixture to obtain a second mixture. The second mixture is fermented aerobically at 31°C for 20 hours, followed by anaerobically fermentation at 26°C for 3 days. The mixture after anaerobically fermenting is filtered, and the filtrate is used as the yeast / rice fermentation product filtrate.

[0029] The preparation method of the bifid yeast fermentation product filtrate comprises: activating Bifidobacterium strains for three generations, culturing the strains in a medium containing glucose, soy peptone, and dipotassium hydrogen phosphate at a pH of 7 and a temperature of 37°C for 20 hours until the bacterial cell density reaches a peak, disrupting the cells at 120 MPa to release intracellular substances, adding lysozyme at a dosage of 0.3 mg / mL at 38°C for 2 hours to promote cell wall disruption, and then centrifuging the lysate to remove bacterial debris. The supernatant is then passed through an ultrafiltration membrane to obtain the bifid yeast fermentation product filtrate. The mass ratio of glucose, soy peptone, and dipotassium hydrogen phosphate is 10:10:3.

[0030] The preparation method of the lactobacillus / soy milk fermentation product filtrate is as follows: soak soybeans for 10 hours, beat them and boil them for 12 minutes, filter and remove the dregs, adjust the soy milk concentration to 9°Bé, sterilize the soy milk at 118°C for 18 minutes, cool it to 38°C, inoculate Lactobacillus plantarum at a rate of 2.5%, and then ferment it at 38°C for 10 hours until the pH value drops to 4.5. The fermentation product is filtered to remove the bacteria and residues, and the filtrate is passed through an ultrafiltration membrane to obtain the lactobacillus / soy milk fermentation product filtrate.

[0031] Examples 2-7, Comparative Examples 1-9 Compared with Example 1, Examples 2-7 and Comparative Examples 1-9 differ in that the mass percentages of hydroxypropyl tetrahydropyrantriol, sodium mannose phosphate, acetyl octapeptide-3, yeast / rice fermentation product filtrate, bifid yeast fermentation product filtrate, and lactobacillus / soymilk fermentation product filtrate are different, as shown in Table 1. The numerical values of each component in Table 1 represent the mass percentage of each component.

[0032] Test Case The compositions of the embodiment and comparative example were prepared into a facial cream, wherein the mass percentage of the compositions in the facial cream was 1.5%.

[0033] Test subjects: 160 volunteers aged 25-45 years with moderate facial pigmentation problems such as melasma or freckles were randomly divided into 16 groups, with 10 people in each group, including 5 males and 5 females.

[0034] 16 groups of volunteers were given the facial creams of the embodiment and the comparative example, once in the morning and once in the evening every day for 8 weeks.

[0035] (1) The skin elasticity tester and wrinkle analyzer were used to measure the elasticity and wrinkle depth of the volunteers' facial skin before and after 8 weeks of use. After use, the average improvement rate of skin elasticity and the average reduction rate of wrinkle depth are shown in Table 1.

[0036] (2) Before using the cream, the skin colorimetry instrument was used to measure the initial skin colorimetry of the test area on the face of all volunteers (the cheek area where the spots are more concentrated, with an area of approximately 4 cm²). After using the cream for 8 weeks, the colorimetry of the same area was measured again, and the amount of improvement in the L value (brightness value, the higher the value, the brighter the skin) was calculated. The average value of each group was taken as the test value. The test results are shown in Table 1.

[0037] Table 1

[0038] As can be seen from Table 1, the composition in the example has better anti-wrinkle and whitening and brightening effects than the comparative example.

[0039] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

[0040] For those skilled in the art, according to the concept of the present invention, there may be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A composition containing bosine and sodium mannose phosphate, characterized in that: The invention comprises the following components in the following mass percentages: 12-25% of hydroxypropyl tetrahydropyrantriol, 0.05-1% of sodium mannose phosphate, 0.001-0.01% of acetyl octapeptide-3, 0.1-0.5% of yeast / rice fermentation product filtrate, 0.01-0.15% of bifid yeast fermentation product filtrate, 0.2-0.5% of lactobacillus / soy milk fermentation product filtrate and the balance of solvent.

2. The composition containing bosine and sodium mannose phosphate according to claim 1, wherein The invention comprises the following components in the following mass percentages: 15-22% of hydroxypropyl tetrahydropyrantriol, 0.1-0.8% of sodium mannose phosphate, 0.003-0.008% of acetyl octapeptide-3, 0.2-0.4% of yeast / rice fermentation product filtrate, 0.03-0.12% of bifid yeast fermentation product filtrate, 0.3-0.45% of lactobacillus / soy milk fermentation product filtrate, and the remainder of solvent.

3. The composition containing bosine and sodium mannose phosphate according to claim 1, wherein The invention comprises the following components in the following mass percentages: 20% of the hydroxypropyl tetrahydropyrantriol, 0.5% of the sodium mannose phosphate, 0.005% of the acetyl octapeptide-3, 0.3% of the yeast / rice fermentation product filtrate, 0.08% of the bifid yeast fermentation product filtrate, 0.4% of the lactobacillus / soy milk fermentation product filtrate, and the remainder of the solvent.

4. The composition containing bosine and sodium mannose phosphate according to claim 1, wherein The solvent includes one or more of water, propylene glycol, butylene glycol and glycerol.

5. The composition containing bosine and sodium mannose phosphate according to any one of claims 1 to 4, characterized in that The preparation method of the yeast / rice fermentation product filtrate includes: mixing rice flour and water with a solid-liquid ratio of 1:5-1:10 to obtain a first mixture; sterilizing the first mixture at 120-130°C for 20-30 minutes, cooling to 25-35°C, and then inoculating yeast, with the yeast accounting for 1-5% by mass of the first mixture, to obtain a second mixture; aerobically fermenting the second mixture at 30-32°C for 12-24 hours, and then anaerobically fermenting it at 25-28°C for 2-5 days; filtering the mixture after the anaerobically fermented mixture, and taking the filtrate as the yeast / rice fermentation product filtrate.

6. The composition containing bosine and sodium mannose phosphate according to any one of claims 1 to 4, characterized in that The preparation method of the bifid yeast fermentation product filtrate comprises: activating bifidobacterium strains for 2-3 generations, culturing the strains in a culture medium comprising glucose, soy peptone and dipotassium hydrogen phosphate at a pH of 6.5-7.5 and a temperature of 36-38° C. anaerobically for 18-24 hours until the bacterial density reaches a peak; after the culturing, breaking the bacterial cells at 100-150 MPa to release intracellular substances; adding lysozyme in an amount of 0.1-0.5 mg / mL and incubating at 37-39° C. for 1-2 hours to promote cell wall fission; then centrifuging the lysate to remove bacterial fragments; and passing the supernatant through an ultrafiltration membrane to obtain the bifid yeast fermentation product filtrate.

7. The composition containing bosine and sodium mannose phosphate according to claim 6, wherein The mass ratio of the glucose, the soy peptone and the dipotassium hydrogen phosphate is 5-20:5-15:1-5.

8. The composition containing bosine and sodium mannose phosphate according to any one of claims 1 to 4, characterized in that The preparation method of the lactobacillus / soymilk fermentation product filtrate comprises the following steps: soaking soybeans for 8-12 hours, beating the soybeans and boiling them for 10-15 minutes, filtering to remove dregs, adjusting the soymilk concentration to 8-10° Bé, sterilizing the soymilk at 115-120° C. for 15-20 minutes, cooling the soymilk to 35-40° C., inoculating plant lactobacillus at an inoculum rate of 2-3%, and then fermenting at 37-40° C. for 8-12 hours until the pH value drops to 4.0-4.5, filtering the fermented product to remove bacteria and residues, and passing the filtrate through an ultrafiltration membrane to obtain the lactobacillus / soymilk fermentation product filtrate.

9. A skin care product, characterized in that: The invention relates to a composition comprising bosine and sodium mannose phosphate according to any one of claims 1 to 8.

10. The skin care product according to claim 9, characterized in that The mass percentage of the composition containing bosine and sodium mannose phosphate in the skin care product is 0.5%-5%.

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