High-dispersity peony seed oil nano-liposome emulsion as well as preparation method and application thereof

Abstract

The invention provides a high-dispersity peony seed oil nano-liposome emulsion as well as a preparation method and application of the high-dispersity peony seed oil nano-liposome emulsion, and relates to the technical field of peony seed oil nano-liposome emulsions. The peony seed oil freeze-dried powder is prepared from the following raw materials in percentage by mass: 10-20% of peony seed oil, 20-30% of phospholipid, 5-10% of cholesterol, 1-5% of cane sugar, 2-5% of Tween and the balance of distilled water. According to the preparation method of the high-dispersity peony seed oil nano-liposome emulsion, a hot melting method, a thin film dispersion method, a micro-emulsification method, homogeneous ultrasonic treatment and freeze drying are combined, water-insoluble peony seed oil can be effectively wrapped in nano-liposome, the water solubility and bioavailability of the nano-liposome are remarkably improved, and the preparation method is simple and convenient. Due to the characteristic, the peony seed oil can be applied to wider fields such as food, cosmetics and medicines, and the nano-liposome structure can enhance the antioxidant stability of the peony seed oil, so that the effective shelf life of the product is prolonged.

Description

Technical Field

[0001] The invention relates to the technical field of peony seed oil nanoliposome emulsion, in particular to a highly dispersible peony seed oil nanoliposome emulsion and a preparation method and application thereof. Background Art

[0002] Peony seed oil is rich in various nutrients, such as unsaturated fatty acids, vitamins, sterols, etc., and has high nutritional value and health benefits. However, the poor water solubility of peony seed oil limits its application in food, medicine, cosmetics and other fields. Nanoliposome emulsion is a carrier system with good dispersibility and biocompatibility, which can effectively improve the solubility, stability and bioavailability of drugs or nutrients. At present, there are many methods for preparing nanoliposome emulsions, but how to efficiently and simply prepare peony seed oil nanoliposome emulsions with high dispersibility and good stability remains an urgent problem to be solved.

[0003] The present invention aims to provide a highly dispersible peony seed oil nanoliposome emulsion, a preparation method and application thereof. Through a specific preparation process, the peony seed oil can be uniformly dispersed in the liposome emulsion, thereby improving its dispersibility and stability, thereby expanding its application in different fields. Summary of the Invention

[0004] To achieve the above object, the present invention provides a highly dispersible peony seed oil nanoliposome emulsion, which is prepared from the following raw materials in percentage by mass: peony seed oil 10-20%, phospholipid 20-30%, cholesterol 5-10%, sucrose 1-5%, Tween 2-5%, and distilled water to make up to 100%;

[0005] The method for preparing the highly dispersible peony seed oil nanoliposome emulsion comprises the following steps:

[0006] Hot melt pretreatment: add phospholipids and cholesterol into a container according to the proportion, and heat in a water bath at 60-70℃ until completely melted;

[0007] Film formation by thin film dispersion method: add peony seed oil to the above-mentioned mixed lipid melt, stir thoroughly, and then evaporate using a rotary evaporator at 40-50°C under reduced pressure;

[0008] Hydration and microemulsification: Add distilled water containing sucrose and Tween to the container where the film is formed, hydrate at 30-40°C, and then microemulsify the crude emulsion under high-speed stirring conditions;

[0009] Homogenization and ultrasonic treatment: The microemulsified emulsion is transferred to a high-pressure homogenizer and homogenized 2-3 times at a pressure of 100-200 MPa, and then the emulsion is ultrasonically treated;

[0010] Freeze drying: The homogenized and ultrasonic emulsion is divided into freeze-drying bottles, the pre-freezing temperature is set to -40--30℃, the pre-freezing time is 2-3 hours, and then freeze-dried under the conditions of vacuum degree less than 10Pa and freeze-drying temperature of -20-0℃.

[0011] In one example, in the hot melt pretreatment, the mixture is heated until completely melted and stirred evenly to form a mixed lipid melt. This step uses the hot melt method to fully mix phospholipids and cholesterol, reduce the interfacial tension between the two, and lay the foundation for the subsequent formation of a stable liposome structure.

[0012] In one example, the thin film dispersion method forms a film, rotary evaporates, removes the organic solvent, and forms a uniform thin film of lipid on the container wall. The thin film dispersion method can arrange the lipid molecules in an orderly manner to form a stable lipid bilayer structure, which is conducive to the encapsulation of peony seed oil.

[0013] In one example, during the hydration and microemulsification, the hydration allows the film to fully absorb water and swell to form a liposome coarse emulsion, and the coarse emulsion is stirred at a high speed of 1000-2000 rpm for 10-20 minutes to further refine the emulsion particles and improve the uniformity of the emulsion.

[0014] In one example, during the homogenization ultrasonic treatment, the microemulsified emulsion is transferred to a high-pressure homogenizer, and the homogenization treatment further refines the emulsion particles. The ultrasonic power of the emulsion ultrasonic treatment is 200-400W, and the ultrasonic time is 10-20 minutes. The cavitation effect and mechanical effect generated by the ultrasound further break up the emulsion particles, making the liposome particle size more uniform and improving the dispersibility of the emulsion.

[0015] In one example, the freeze-drying method obtains highly dispersible peony seed oil nanoliposome freeze-dried powder. When used, the freeze-dried powder can be re-dissolved in water as needed to obtain a highly dispersible peony seed oil nanoliposome emulsion.

[0016] In one example, when the lyophilized powder is reconstituted, warm water at 30-40°C is used and stirring is continued for 15-30 minutes at a stirring speed of 500-1000 rpm. After reconstitution, the particle size distribution of the emulsion is maintained at 50-150 nm, ensuring the integrity and dispersibility of the nanoliposome structure.

[0017] In one example, sucrose and Tween added to the nanoliposome emulsion act synergistically, sucrose acts as a freeze-drying protectant to prevent the liposome structure from being destroyed, and Tween acts as a surfactant to enhance the physical stability of the emulsion.

[0018] The invention discloses an application of a highly dispersible peony seed oil nanoliposome emulsion, characterized in that the emulsion is used in the field of transdermal drug delivery systems or food additives, wherein the nanoliposome structure can improve the antioxidant stability of the peony seed oil and enhance skin permeability or oral absorption efficiency through the synergistic effect of phospholipids and cholesterol.

[0019] The highly dispersible peony seed oil nanoliposome emulsion and its preparation method and application proposed by the present invention can bring the following beneficial effects:

[0020] The highly dispersible peony seed oil nanoliposome emulsion proposed by the present invention and its preparation method and application can bring about multiple beneficial effects:

[0021] 1. The present invention combines multiple methods such as hot melt method, thin film dispersion method, microemulsification method, homogenization ultrasound and freeze drying to effectively encapsulate water-insoluble peony seed oil in nanoliposomes, significantly improving its water solubility and bioavailability. This feature enables peony seed oil to be applied in a wider range of fields, such as food, cosmetics and medicine. The nanoliposome structure can enhance the antioxidant stability of peony seed oil, thereby extending the effective shelf life of the product.

[0022] 2. In the transdermal drug delivery system of the present invention, the nanoliposome structure can effectively enhance the skin's absorption of nutrients and enhance skin permeability through the synergistic effect of phospholipids and cholesterol, thereby improving the use effect of skin care products. In oral preparations, the nanoliposomes can enhance the digestion and absorption efficiency of peony seed oil, making the nutrients more easily absorbed by the human body, thereby improving the efficacy of drugs and health products. The preparation method is simple, the operating conditions are mild, and it is suitable for large-scale production, which provides convenience for subsequent industrial applications.

[0023] 3. The peony seed oil nanoliposome emulsion prepared by the present invention has a small and uniform particle size with an average particle size between 50 and 150 nm, which makes it difficult for agglomeration or stratification to occur during storage, thereby ensuring its long-term stability. Due to its good dispersibility, stability and biocompatibility, this highly dispersible peony seed oil nanoliposome emulsion can be applied to multiple fields, including food, cosmetics and medicine. During the preparation and reconstitution of the freeze-dried powder, the added sucrose and Tween respectively play a role in preventing the destruction of the liposome structure and enhancing the physical stability of the emulsion, further ensuring the functionality and practicality of the product. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0025] Figure 1The present invention is a schematic flow chart of a method for preparing a highly dispersible peony seed oil nanoliposome emulsion. DETAILED DESCRIPTION

[0026] In order to more clearly and completely illustrate the technical solution of the present invention, the present invention will be further described below with reference to the accompanying drawings.

[0027] Please refer to Figure 1 The present invention provides a highly dispersible peony seed oil nanoliposome emulsion, which is prepared from the following raw materials in percentage by mass: 10-20% peony seed oil, 20-30% phospholipids, 5-10% cholesterol, 1-5% sucrose, 2-5% Tween, and distilled water to 100%. The preparation method of the highly dispersible peony seed oil nanoliposome emulsion includes the following steps:

[0028] Hot melt pretreatment: Add phospholipids and cholesterol into a container in proportion, heat in a water bath at 60-70°C until completely melted, and stir evenly to form a mixed lipid melt. This step uses the hot melt method to fully mix phospholipids and cholesterol, reduce the interfacial tension between the two, and lay the foundation for the subsequent formation of a stable liposome structure.

[0029] Film formation by thin film dispersion method: peony seed oil is added to the above-mentioned mixed lipid melt, and after thorough stirring, the organic solvent is removed by rotary evaporation at 40-50°C under reduced pressure using a rotary evaporator to form a uniform thin film on the container wall. The thin film dispersion method can orderly arrange the lipid molecules to form a stable lipid bilayer structure, which is conducive to the encapsulation of peony seed oil.

[0030] Hydration and microemulsification: Add distilled water containing sucrose and Tween to the container where the film is formed, and hydrate it at 30-40°C to allow the film to fully absorb water and swell to form a liposome crude emulsion. The crude emulsion is then microemulsified under high-speed stirring conditions at a speed of 1000-2000 rpm for 10-20 minutes to further refine the emulsion particles and improve the uniformity of the emulsion.

[0031] Homogenization ultrasonic treatment: The microemulsified emulsion is transferred to a high-pressure homogenizer and homogenized 2-3 times at a pressure of 100-200 MPa to further refine the emulsion particles. The emulsion is then ultrasonically treated with an ultrasonic power of 200-400 W and an ultrasonic time of 10-20 minutes. The cavitation effect and mechanical effect generated by ultrasound further break up the emulsion particles, making the liposome particle size more uniform and improving the dispersibility of the emulsion.

[0032] Freeze drying: The emulsion after homogenization and ultrasound is divided into freeze-drying bottles, the pre-freezing temperature is set to -40--30°C, the pre-freezing time is 2-3 hours, and then freeze-drying is carried out under the conditions of vacuum degree less than 10Pa and freeze-drying temperature of -20-0°C to obtain highly dispersible peony seed oil nanoliposome freeze-dried powder. When used, the freeze-dried powder can be re-dissolved in water as needed to obtain highly dispersible peony seed oil nanoliposome emulsion.

[0033] Example 1

[0034] Prepare raw materials: Weigh 7 g of egg yolk lecithin, 1 g of cholesterol, and 1.2 g of peony seed oil. The mass ratio of egg yolk lecithin, cholesterol, and peony seed oil is 7:1:1.2. Prepare 50 mL of anhydrous ethanol, 0.5 g of sucrose, 0.5 g of Tween, and 100 g of distilled water.

[0035] Preparation of lipid film (thin film dispersion method): Egg yolk lecithin and cholesterol were dissolved in anhydrous ethanol. The concentrations of egg yolk lecithin and cholesterol in anhydrous ethanol were 12 mg / mL and 1.5 mg / mL, respectively. The lipid solution was poured into a round-bottom flask and rotary evaporated on a rotary evaporator at 45°C and 100 rpm to remove the anhydrous ethanol and form a uniform lipid film on the inner wall of the round-bottom flask.

[0036] Preparation of oil phase (hot melt method): Heat the peony seed oil to 60°C to completely melt it to obtain the oil phase. Stir continuously during the heating process to ensure that the peony seed oil is evenly heated.

[0037] Merging and primary emulsification: Add the formed lipid film to 50 mL of hot water at 55°C, shake in a water bath to remove the lipid film and form a lipid suspension. The mass ratio of lipid to water is 1:10, and the speed of water bath shaking is controlled in a moderate range for about 15 minutes to allow the lipid film to fully fall off and form a uniform suspension. Slowly add the oil phase to the lipid suspension and stir at 60°C and 1000 rpm for 10 minutes to form colostrum. Pay attention to controlling the speed and temperature during stirring to prevent emulsion stratification or aggregation.

[0038] Hydration and microemulsification: Add distilled water containing sucrose and Tween to the container where the film is formed, and hydrate it at 30-40°C to allow the film to fully absorb water and swell to form a liposome crude emulsion. The crude emulsion is then microemulsified under high-speed stirring conditions at a speed of 1000-2000 rpm for 10-20 minutes to further refine the emulsion particles and improve the uniformity of the emulsion.

[0039] Homogenization ultrasonic treatment: The microemulsified emulsion is transferred to a high-pressure homogenizer and homogenized 2-3 times at a pressure of 100-200 MPa. The emulsion is then ultrasonically treated with an ultrasonic power of 200-400 W and an ultrasonic time of 10-20 minutes. The cavitation effect and mechanical effect generated by ultrasound further break up the emulsion particles, making the liposome particle size more uniform and improving the dispersibility of the emulsion.

[0040] Freeze drying: The emulsion after homogenization and ultrasound is divided into freeze-drying bottles, the pre-freezing temperature is set to -40--30°C, the pre-freezing time is 2-3 hours, and then freeze-drying is carried out under the conditions of vacuum degree less than 10Pa and freeze-drying temperature of -20-0°C to obtain highly dispersible peony seed oil nanoliposome freeze-dried powder. When used, the freeze-dried powder can be re-dissolved in water as needed to obtain highly dispersible peony seed oil nanoliposome emulsion.

[0041] Example 2

[0042] Prepare raw materials: Weigh 6 g of egg yolk lecithin, 1 g of cholesterol, and 1 g of peony seed oil in a mass ratio of 6:1:1. Prepare 40 mL of anhydrous ethanol, 0.4 g of sucrose, 0.4 g of Tween, and 100 g of distilled water.

[0043] Preparation of lipid film (thin film dispersion method): Egg yolk lecithin and cholesterol were dissolved in anhydrous ethanol. The concentrations of egg yolk lecithin and cholesterol in anhydrous ethanol were 10 mg / mL and 1 mg / mL, respectively. The lipid solution was poured into a round-bottom flask and rotary evaporated on a rotary evaporator at 40°C and 80 rpm to remove the anhydrous ethanol and form a uniform lipid film on the inner wall of the round-bottom flask.

[0044] Preparation of oil phase (hot melt method): Heat the peony seed oil to 80°C to completely melt it to obtain the oil phase. Stir continuously during the heating process to ensure that the peony seed oil is evenly heated.

[0045] Merging and primary emulsification: Add the formed lipid film to 40 mL of hot water at 50°C, and shake in a water bath to remove the lipid film to form a lipid suspension. The mass ratio of lipid to water is 1:8. The speed of water bath shaking is controlled in a moderate range for about 10 minutes to allow the lipid film to fully fall off and form a uniform suspension. Slowly add the oil phase to the lipid suspension and stir at 60°C and 1000 rpm for 10 minutes to form colostrum. Pay attention to controlling the speed and temperature during stirring to prevent emulsion stratification or aggregation.

[0046] Hydration and microemulsification: Add distilled water containing sucrose and Tween to the container where the film is formed, and hydrate it at 30-40°C to allow the film to fully absorb water and swell to form a liposome crude emulsion. The crude emulsion is then microemulsified under high-speed stirring conditions at a speed of 1000-2000 rpm for 10-20 minutes to further refine the emulsion particles and improve the uniformity of the emulsion.

[0047] Homogenization ultrasonic treatment: The microemulsified emulsion is transferred to a high-pressure homogenizer and homogenized 2-3 times at a pressure of 100-200 MPa. The emulsion is then ultrasonically treated with an ultrasonic power of 200-400 W and an ultrasonic time of 10-20 minutes. The cavitation effect and mechanical effect generated by ultrasound further break up the emulsion particles, making the liposome particle size more uniform and improving the dispersibility of the emulsion.

[0048] Freeze drying: The emulsion after homogenization and ultrasound is divided into freeze-drying bottles, the pre-freezing temperature is set to -40--30°C, the pre-freezing time is 2-3 hours, and then freeze-drying is carried out under the conditions of vacuum degree less than 10Pa and freeze-drying temperature of -20-0°C to obtain highly dispersible peony seed oil nanoliposome freeze-dried powder. When used, the freeze-dried powder can be re-dissolved in water as needed to obtain highly dispersible peony seed oil nanoliposome emulsion.

[0049] Example 3

[0050] Prepare raw materials: Weigh 8 g of egg yolk lecithin, 1 g of cholesterol, and 1.5 g of peony seed oil in a mass ratio of 8:1:1.5. Prepare 60 mL of anhydrous ethanol, 0.6 g of sucrose, 0.6 g of Tween, and 100 g of distilled water.

[0051] Preparation of lipid film (thin film dispersion method): Egg yolk lecithin and cholesterol were dissolved in anhydrous ethanol. The concentrations of egg yolk lecithin and cholesterol in anhydrous ethanol were 15 mg / mL and 2 mg / mL, respectively. The lipid solution was poured into a round-bottom flask and rotary evaporated on a rotary evaporator at 50°C and 120 rpm to remove the anhydrous ethanol and form a uniform lipid film on the inner wall of the round-bottom flask.

[0052] Preparation of oil phase (hot melt method): Heat the peony seed oil to 60°C to completely melt it to obtain the oil phase. Stir continuously during the heating process to ensure that the peony seed oil is evenly heated.

[0053] Merging and primary emulsification: Add the formed lipid film to 60 mL of hot water at 60°C, shake in a water bath to remove the lipid film and form a lipid suspension. The mass ratio of lipid to water is 1:12. The speed of water bath shaking is controlled in a moderate range for about 20 minutes to allow the lipid film to fully fall off and form a uniform suspension. Slowly add the oil phase to the lipid suspension and stir at 70°C and 1500 rpm for 20 minutes to form colostrum. Pay attention to controlling the speed and temperature during stirring to prevent emulsion stratification or aggregation.

[0054] Hydration and microemulsification: Add distilled water containing sucrose and Tween to the container where the film is formed, and hydrate it at 30-40°C to allow the film to fully absorb water and swell to form a liposome crude emulsion. The crude emulsion is then microemulsified under high-speed stirring conditions at a speed of 1000-2000 rpm for 10-20 minutes to further refine the emulsion particles and improve the uniformity of the emulsion.

[0055] Homogenization ultrasonic treatment: The microemulsified emulsion is transferred to a high-pressure homogenizer and homogenized 2-3 times at a pressure of 100-200 MPa. The emulsion is then ultrasonically treated with an ultrasonic power of 200-400 W and an ultrasonic time of 10-20 minutes. The cavitation effect and mechanical effect generated by ultrasound further break up the emulsion particles, making the liposome particle size more uniform and improving the dispersibility of the emulsion.

[0056] Freeze drying: The emulsion after homogenization and ultrasound is divided into freeze-drying bottles, the pre-freezing temperature is set to -40--30°C, the pre-freezing time is 2-3 hours, and then freeze-drying is carried out under the conditions of vacuum degree less than 10Pa and freeze-drying temperature of -20-0°C to obtain highly dispersible peony seed oil nanoliposome freeze-dried powder. When used, the freeze-dried powder can be re-dissolved in water as needed to obtain highly dispersible peony seed oil nanoliposome emulsion.

[0057] In the present embodiment, the detailed operation process of the above-mentioned different embodiments is used to fully verify the feasibility and effectiveness of the method for preparing the highly dispersible peony seed oil nanoliposome emulsion proposed by the present invention. From the raw material preparation stage, the accurate weighing ratio of different components is used, and the strict control of parameters such as temperature, time, rotation speed, and pressure in each step such as thin film dispersion method and hot melt method is carried out, all in order to prepare nanoliposome emulsion with excellent performance. In Examples 1-3, different raw material ratios and process parameter settings simulate a variety of possible preparation conditions. The results show that each embodiment has successfully prepared peony seed oil nanoliposome emulsion, and performs well in terms of stability and dispersibility. This shows that the preparation method of the present invention has good adaptability and can flexibly adjust parameters within a certain range to meet different production needs. Further analysis shows that in the thin film dispersion method, different rotary evaporation temperatures, rotation speeds and lipid concentrations in ethanol affect the formation quality of the lipid film. Lower temperatures and rotation speeds may make the lipid film formation speed slower, but can ensure its uniformity; higher temperatures and rotation speeds can accelerate the formation of the lipid film, but it is important to prevent lipid from being excessively formed. In the hot melt method, the heating temperature and stirring degree of peony seed oil ensure its full melting and uniform heating, laying the foundation for subsequent mixing with lipid suspension. In the hydration and microemulsification steps, the temperature of hot water, the time and speed of water bath shaking determine whether the lipid film can be fully detached and form a uniform suspension. The speed and time of high-speed stirring directly affect the quality of colostrum. In the homogenization and refinement stage, the choice of homogenization pressure and number of times plays a key role in the uniformity and stability of the emulsion particle size. Properly increasing the pressure and increasing the number of times can make the emulsion particle size smaller and more uniform. During the preparation of the aqueous phase and the mixing and adjustment process, factors such as the amount of glycerol added, the preheating temperature of the aqueous phase, and the temperature, rotation speed, and time during mixing jointly affect the final properties of the emulsion, such as the regulation of osmotic pressure and the stability of the emulsion. In addition, the addition of preservatives and antioxidants of different types and amounts in the embodiments provides a guarantee for the long-term preservation of the product in different application scenarios. This also reflects the flexibility and scalability of the present invention in practical applications. The formula can be adjusted according to specific needs to meet the requirements of product stability and shelf life in different fields such as food, cosmetics, and medicine.

[0058] Of course, the present invention may have many other implementations. Based on this implementation, other implementations obtained by ordinary technicians in this field without any creative work are all within the scope of protection of the present invention.

Claims

1. A highly dispersible peony seed oil nanoliposome emulsion, prepared from the following raw materials in percentage by weight: peony seed oil 10-20%, phospholipids 20-30%, cholesterol 5-10%, sucrose 1-5%, Tween 2-5%, and distilled water to 100%; The method for preparing the highly dispersible peony seed oil nanoliposome emulsion comprises the following steps: Hot Melt Pretreatment: Add phospholipids and cholesterol into a container according to the proportion, and heat in a water bath at 60-70℃ until completely melted; Film formation by thin film dispersion method: add peony seed oil to the above-mentioned mixed lipid melt, stir thoroughly, and then evaporate using a rotary evaporator at 40-50°C under reduced pressure; Hydration and microemulsification: Add distilled water containing sucrose and Tween to the container where the film is formed, hydrate at 30-40°C, and then microemulsify the crude emulsion under high-speed stirring conditions; Homogenization and ultrasonic treatment: The microemulsified emulsion is transferred to a high-pressure homogenizer and homogenized 2-3 times at a pressure of 100-200 MPa, and then the emulsion is ultrasonically treated; Freeze drying: The homogenized and ultrasonic emulsion is divided into freeze-drying bottles, the pre-freezing temperature is set to -40--30℃, the pre-freezing time is 2-3 hours, and then freeze-dried under the conditions of vacuum degree less than 10Pa and freeze-drying temperature of -20-0℃.

2. A highly dispersible peony seed oil nanoliposome emulsion and a preparation method thereof according to claim 1, characterized in that, In the hot melt pretreatment, the phospholipids and cholesterol are heated until completely melted and stirred evenly to form a mixed lipid melt. This step utilizes the hot melt method to fully mix the phospholipids and cholesterol, reduce the interfacial tension between the two, and lay the foundation for the subsequent formation of a stable liposome structure.

3. A highly dispersible peony seed oil nanoliposome emulsion and preparation method thereof according to claim 1, characterized in that, The thin film dispersion method forms a film, and rotary evaporation removes the organic solvent, so that the lipid forms a uniform thin film on the container wall. The thin film dispersion method can arrange the lipid molecules in an orderly manner to form a stable lipid bilayer structure, which is conducive to encapsulating the peony seed oil.

4. A highly dispersible peony seed oil nanoliposome emulsion and a preparation method thereof according to claim 1, characterized in that, During the hydration and microemulsification, the hydration allows the film to fully absorb water and swell to form a liposome coarse emulsion, and the coarse emulsion is stirred at a high speed of 1000-2000 rpm for 10-20 minutes to further refine the emulsion particles and improve the uniformity of the emulsion.

5. A highly dispersible peony seed oil nanoliposome emulsion and a preparation method thereof according to claim 1, characterized in that, During the homogenization ultrasonic treatment, the microemulsified emulsion is transferred to a high-pressure homogenizer, and the homogenization treatment further refines the emulsion particles. The ultrasonic power of the emulsion ultrasonic treatment is 200-400W, and the ultrasonic time is 10-20 minutes. The cavitation effect and mechanical effect generated by the ultrasound further break up the emulsion particles, making the liposome particle size more uniform and improving the dispersibility of the emulsion.

6. A highly dispersible peony seed oil nanoliposome emulsion and a preparation method thereof according to claim 1, characterized in that, The freeze-drying method obtains highly dispersible peony seed oil nanoliposome freeze-dried powder. When used, the freeze-dried powder can be re-dissolved in water as needed to obtain a highly dispersible peony seed oil nanoliposome emulsion.

7. A highly dispersible peony seed oil nanoliposome emulsion and a preparation method thereof according to claim 6, characterized in that, When the freeze-dried powder is reconstituted, warm water at 30-40° C. is used and stirring is continued for 15-30 minutes at a stirring speed of 500-1000 rpm. The particle size distribution of the emulsion after reconstitution is maintained at 50-150 nm, ensuring the integrity and dispersibility of the nanoliposome structure.

8. A highly dispersible peony seed oil nanoliposome emulsion and a preparation method thereof according to claim 7, characterized in that, The sucrose and Tween added to the nano-liposome emulsion act synergistically. The sucrose acts as a freeze-drying protective agent to prevent the liposome structure from being destroyed, and the Tween acts as a surfactant to enhance the physical stability of the emulsion.

9. The use of a highly dispersible peony seed oil nanoliposome emulsion according to claim 1-8, characterized in that, The emulsion is used in the field of transdermal drug delivery systems or food additives, wherein the nanoliposome structure can improve the antioxidant stability of peony seed oil and enhance skin permeability or oral absorption efficiency through the synergistic effect of phospholipids and cholesterol.

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