A traditional Chinese medicine moxa stick for consolidating kidney and cultivating primordial qi and a preparation method thereof

Abstract

The application belongs to the technical field of moxa processing, and particularly relates to a traditional Chinese medicine moxa for consolidating kidney and cultivating primordial qi and a preparation method thereof. The preparation method of the traditional Chinese medicine moxa comprises the following steps: (1) dispersing moxa in water, filtering and drying after ultrasonic treatment, and reserving; (2) mixing traditional Chinese medicine ingredients according to weight, crushing and sieving to obtain traditional Chinese medicine powder; (3) mixing modified pullulan and broussonetia papyrifera bark powder to obtain an adhesive; (4) adding the adhesive into water, and then adding the traditional Chinese medicine powder to mix uniformly to obtain a mixture; and (5) adding the moxa into the mixture, uniformly mixing, and then compressing and drying to obtain the moxa. The traditional Chinese medicine moxa obtained through the preparation method can make the medicine powder adhere to the moxa when burning, and solves the problems that the medicine powder is easy to fall off when burning, and the effective components of traditional Chinese medicine are not uniformly released. Moreover, the process steps are simple, the conditions are mild, and the use safety of the moxa is not affected when fully burning.

Description

Technical Field

[0001] This invention belongs to the field of moxa stick processing technology, specifically relating to a traditional Chinese medicine moxa stick for strengthening the kidneys and nourishing the body, and its preparation method. Background Technology

[0002] Today's fast-paced lifestyle places immense physical and mental stress on most people, leading to symptoms such as insomnia, fatigue, irritability, and irregular sleep patterns, all of which can cause kidney deficiency. For those with kidney deficiency, moxibustion is an excellent treatment method. Mugwort is a pure yang plant; during moxibustion, it warms the body, replenishes yang energy, and expels cold from the body. It is an ancient Chinese natural health therapy with no side effects and is easy to perform.

[0003] Traditional moxibustion primarily relies on the warming effect of pure moxa wool. While it has the effects of warming the meridians, promoting blood circulation, and tonifying the middle energizer, its effectiveness in treating specific symptoms such as kidney yang deficiency is limited. Current moxibustion methods primarily use pure moxa sticks for health maintenance. There are also herbal moxa sticks, but these are not highly targeted, use expensive herbs, and are costly, making them unsuitable for the needs of ordinary families for disease prevention and treatment. Moxa wool is obtained by repeatedly pounding, crushing, and sifting the leaves of the Artemisia argyi plant (a member of the Asteraceae family) to remove impurities and dust. It is soft like wool, easily ignited without flame, and has a fragrant aroma. Moxa wool is the raw material for making moxa sticks and the main material used in moxibustion. It has the effects of promoting blood circulation, warming the meridians, stopping bleeding, dispelling cold, relieving pain, and maintaining health. Currently, most commercially available herbal moxa sticks mix moxa wool with other Chinese medicinal herbs. When the moxa stick burns, the active ingredients in the herbs volatilize and are absorbed through the skin to the affected area. During moxibustion, some moxa sticks contain non-flammable Chinese medicinal herbs that cannot burn completely, resulting in poor moxibustion effects and producing a large amount of irritating smoke, affecting the experience. The medicinal powder is easy to fall off during combustion, leading to uneven release of effective ingredients and reducing efficacy. On the other hand, although some moxa sticks can burn completely, the ash after burning is easy to fall off, and the fallen ash can easily burn the skin, resulting in many defects in use.

[0004] To achieve the above objectives, the present invention provides a traditional Chinese medicine moxa stick for strengthening the kidneys and nourishing vital energy, and a method for preparing the same. Summary of the Invention

[0005] The primary objective of this invention is to provide a method for preparing moxa sticks for strengthening the kidneys and nourishing vital energy.

[0006] The second objective of this invention is to provide a traditional Chinese medicine moxa stick for strengthening the kidneys and nourishing vital energy.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0008] A method for preparing a traditional Chinese medicine moxa stick for strengthening the kidneys and nourishing vital energy includes the following steps:

[0009] (1) Disperse the mugwort floss in water, treat it with ultrasound, filter and dry it for later use;

[0010] (2) Mix the Chinese herbal ingredients Cistanche deserticola, Epimedium, Astragalus membranaceus, Ligusticum chuanxiong and Angelica sinensis, pulverize and sieve to obtain Chinese herbal powder;

[0011] (3) The modified pullulan polysaccharide and paper mulberry bark powder were mixed in a mass ratio of 1:(1-3) to obtain an adhesive;

[0012] (4) Add deionized water to the adhesive obtained in step (3), and then add the Chinese herbal powder obtained in step (2) and mix evenly to obtain a mixture;

[0013] (5) Add moxa wool to the mixture obtained in step (4), mix thoroughly and evenly, then press and dry to obtain moxa sticks.

[0014] Furthermore, the preparation process of the modified pullulan is as follows: after mixing pullulan aqueous solution with dilute sulfuric acid, sodium periodate aqueous solution is added dropwise, stirred in the dark, and after removing sodium periodate, it is freeze-dried to obtain oxidized pullulan; the oxidized pullulan is dissolved in anhydrous ethanol, and then theanine is added and mixed to carry out the reaction to obtain modified pullulan.

[0015] Further, the concentration of the dilute sulfuric acid is 0.08-0.12 mol / L; the molar ratio of pullulan to sodium periodate is 1:(1-2); the stirring time in the dark is 4-6 h; the freeze-drying time is 48-72 h; the molar ratio of oxidized pullulan to theanine is 1:(1.1-1.5); and the reaction temperature after adding theanine is 60-80℃, and the reaction time is 2-4 h.

[0016] Furthermore, in step (1), the frequency of ultrasonic treatment is 20-50KHZ, the power is 400-600W, and the time is 1-2h.

[0017] Furthermore, the weight proportions of each Chinese herbal medicine component in step (2) are as follows: 8-12 parts of Cistanche deserticola, 5-10 parts of Epimedium, 5-10 parts of Astragalus membranaceus, 3-5 parts of Ligusticum chuanxiong, and 1-4 parts of Angelica sinensis.

[0018] Furthermore, in step (3), the mass ratio of modified pullulan to paper mulberry bark powder is 1:(1-3).

[0019] Furthermore, in step (4), the volume ratio of the adhesive to the deionized water is 1:(1-3).

[0020] Furthermore, the drying in step (5) is atmospheric pressure drying at a temperature of 60-80℃.

[0021] A traditional Chinese medicine moxa stick for strengthening the kidneys and nourishing vital energy is prepared according to the preparation method described above.

[0022] Compared with the prior art, the main advantages of the present invention are as follows:

[0023] This invention uses mugwort floss as raw material, adds traditional Chinese medicine with kidney-tonifying and vital energy-nourishing properties as excipients, and uses modified pullulan polysaccharide and mulberry bark powder as binders. The resulting herbal moxa stick allows the medicinal powder to adhere firmly to the mugwort floss, solving the problems of easy powder detachment during combustion and uneven release of effective components of traditional Chinese medicine. Furthermore, the process is simple, the conditions are mild, and complete combustion does not affect the safety of the moxa stick. Attached Figure Description

[0024] Figure 1 Morphological diagram of fibroblasts cultured in DMEM / F12 medium containing the mugwort smoke-infused liquid prepared by burning the mugwort sticks prepared in Example 1;

[0025] Figure 2 Morphological image of fibroblasts cultured in DMEM / F12 medium. Detailed Implementation

[0026] The technical solution of the present invention will be further described below with reference to specific embodiments. However, those skilled in the art should understand that the following embodiments are only for illustrating the present invention and should not be regarded as limiting the present invention. Specific conditions not specified in the embodiments are performed according to conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the reagents or instruments used are all conventional products obtained through commercial channels.

[0027] Example 1

[0028] A method for preparing a traditional Chinese medicine moxa stick for strengthening the kidneys and nourishing vital energy includes the following steps:

[0029] (1) Take moxa wool and disperse it in water (the mass ratio of moxa wool to water is 1:2), mix it, treat it with ultrasound (frequency is 35HZ, power is 500W, time is 2h), filter it, dry it and set it aside;

[0030] (2) Weigh out 10 parts of the Chinese herbal ingredients Cistanche deserticola, 7 parts of Epimedium, 7 parts of Astragalus membranaceus, 4 parts of Ligusticum chuanxiong, and 3 parts of Angelica sinensis according to their weight, mix them, pulverize and sieve to obtain Chinese herbal powder;

[0031] (3) A certain amount of pullulan polysaccharide aqueous solution was mixed with 0.1 mol / L dilute sulfuric acid, the pH was adjusted to 4, and sodium periodate aqueous solution was added dropwise. The molar ratio of pullulan polysaccharide to sodium periodate was 1:1.5. The solution was stirred continuously for 5 hours under light-protected conditions to remove sodium periodate, and then freeze-dried for 60 hours to obtain oxidized pullulan polysaccharide. The oxidized pullulan polysaccharide was dissolved in anhydrous ethanol solvent, and theanine was added and mixed. The molar ratio of oxidized pullulan polysaccharide to theanine was 1:1.3. Dilute hydrochloric acid was added dropwise, and the reaction was carried out at 70℃ for 3 hours. The anhydrous ethanol was removed by rotary evaporation to obtain modified pullulan polysaccharide. The modified pullulan polysaccharide was mixed with paper mulberry bark powder at a mass ratio of 1:2 to obtain an adhesive.

[0032] (4) Add the adhesive obtained in step (3) to water (the mass ratio of adhesive to deionized water is 1:2), then add the Chinese medicine powder obtained in step (2) and mix evenly to obtain a mixture;

[0033] (5) Add the moxa wool to the mixture obtained in step (4), mix thoroughly and evenly, press and dry at normal pressure, and dry at 70℃ to obtain moxa sticks.

[0034] The preparation method of the paper mulberry bark powder mainly includes the following steps: First, collect the bark of healthy paper mulberry trees, clean it, cut it into 1-5 mm sections, put it into a 10% NaOH solution, soak it at 80℃ for 40 min, remove the NaOH solution, wash it with water until neutral, dry it, and then pulverize it.

[0035] This embodiment also provides a traditional Chinese medicine moxa stick for strengthening the kidneys and nourishing the body, prepared by the above method.

[0036] Example 2

[0037] A method for preparing a traditional Chinese medicine moxa stick for strengthening the kidneys and nourishing vital energy includes the following steps:

[0038] (1) Take moxa wool and disperse it in water (the mass ratio of moxa wool to water is 1:2), mix it, treat it with ultrasound (frequency is 20HZ, power is 400W, time is 1h), filter it, dry it and set it aside;

[0039] (2) Weigh out 8 parts of the Chinese herbal ingredients Cistanche deserticola, 5 parts of Epimedium, 5 parts of Astragalus membranaceus, 3 parts of Ligusticum chuanxiong, and 1 part of Angelica sinensis according to their weight, mix them, pulverize and sieve to obtain Chinese herbal powder;

[0040] (3) A certain amount of pullulan polysaccharide aqueous solution was mixed with 0.1 mol / L dilute sulfuric acid, the pH was adjusted to 4, and sodium periodate aqueous solution was added dropwise. The molar ratio of pullulan polysaccharide to sodium periodate was 1:1. The solution was stirred continuously for 4 hours under light-protected conditions to remove sodium periodate, and then freeze-dried for 48 hours to obtain oxidized pullulan polysaccharide. The oxidized pullulan polysaccharide was dissolved in anhydrous ethanol solvent, and theanine was added and mixed. The molar ratio of oxidized pullulan polysaccharide to theanine was 1:1.1. Dilute hydrochloric acid was added dropwise, and the reaction was carried out at 60℃ for 2 hours. The anhydrous ethanol was removed by rotary evaporation to obtain modified pullulan polysaccharide. The modified pullulan polysaccharide was mixed with paper mulberry bark powder at a mass ratio of 1:1 to obtain an adhesive.

[0041] (4) Add the adhesive obtained in step (3) to water (the mass ratio of adhesive to deionized water is 1:1), then add the Chinese herbal powder obtained in step (2) and mix evenly to obtain a mixture;

[0042] (5) Add the moxa wool to the mixture obtained in step (4), mix thoroughly and evenly, press and dry at normal pressure, and dry at 60℃ to obtain moxa sticks.

[0043] The preparation method of the paper mulberry bark powder is as shown in Example 1.

[0044] This embodiment also provides a traditional Chinese medicine moxa stick for strengthening the kidneys and nourishing the body, prepared by the above method.

[0045] Example 3

[0046] A method for preparing a traditional Chinese medicine moxa stick for strengthening the kidneys and nourishing vital energy includes the following steps:

[0047] (1) Take moxa wool and disperse it in water (the mass ratio of moxa wool to water is 1:2), mix it, treat it with ultrasound (frequency is 50HZ, power is 600W, time is 2h), filter it, dry it and set it aside;

[0048] (2) Weigh out 12 parts of the Chinese herbal ingredients Cistanche deserticola, 10 parts of Epimedium, 10 parts of Astragalus membranaceus, 5 parts of Ligusticum chuanxiong, and 4 parts of Angelica sinensis according to their weight, mix them, pulverize and sieve to obtain Chinese herbal powder;

[0049] (3) A certain amount of pullulan polysaccharide aqueous solution was mixed with 0.1 mol / L dilute sulfuric acid, the pH was adjusted to 5, and sodium periodate aqueous solution was added dropwise. The molar ratio of pullulan polysaccharide to sodium periodate was 1:2. The solution was stirred continuously for 5 h under light-protected conditions to remove sodium periodate, and then freeze-dried for 72 h to obtain oxidized pullulan polysaccharide. The oxidized pullulan polysaccharide was dissolved in anhydrous ethanol solvent, and theanine was added and mixed. The molar ratio of oxidized pullulan polysaccharide to theanine was 1:1.5. Dilute hydrochloric acid was added dropwise, and the reaction was carried out at 80℃ for 4 h. The anhydrous ethanol was removed by rotary evaporation to obtain modified pullulan polysaccharide. The modified pullulan polysaccharide was mixed with paper mulberry bark powder at a mass ratio of 1:2 to obtain an adhesive.

[0050] (4) Add the adhesive obtained in step (3) to water (the mass ratio of adhesive to deionized water is 1:3), then add the Chinese medicine powder obtained in step (2) and mix evenly to obtain a mixture;

[0051] (5) Add the moxa wool to the mixture obtained in step (4), mix thoroughly and evenly, press and dry at normal pressure, and dry at 80℃ to obtain moxa sticks.

[0052] The preparation method of the paper mulberry bark powder is as shown in Example 1.

[0053] This embodiment also provides a traditional Chinese medicine moxa stick for strengthening the kidneys and nourishing the body, prepared by the above method.

[0054] Comparative Example 1

[0055] The difference between Comparative Example 1 and Example 1 is that the modified pullulan in step (3) is replaced with pullulan.

[0056] Comparative Example 2

[0057] The difference between Comparative Example 2 and Example 1 is that the modified pullulan in step (3) is omitted.

[0058] Comparative Example 3

[0059] The difference between Comparative Example 3 and Example 1 is that the paper mulberry bark powder in step (3) is omitted.

[0060] Experimental Example 1

[0061] Take the moxa sticks prepared in Examples 1-3 and Comparative Examples 1-3 respectively, and place them in a vibrating sieve equipped with a single-layer screen. The sieve particle size is 0.15 mm, the amplitude is 5 mm, and the mass of the material passing through the sieve is weighed after 5 minutes. Repeat the test 3 times, take the average value, and calculate the sieve drop rate. The formula for calculating the sieve drop rate is as follows: Sieve drop rate = (Mass of material passing through the sieve / Mass of medicinal powder contained in the moxa stick) × 100%. The results are shown in Table 1.

[0062] Table 1 Screening failure rate

[0063]

[0064] As can be seen from Table 1, compared with Comparative Examples 1, 2, and 3, the moxa sticks prepared in Examples 1-3 of this invention have a relatively low sieve loss rate. This indicates that the binder prepared by adding water to the modified pullulan polysaccharide and paper mulberry bark powder in Examples 1-3 plays a very important role, enabling the medicinal powder to adhere firmly to the moxa wool, thus solving the problem of the medicinal powder easily falling off during combustion and causing uneven release of the effective components of traditional Chinese medicine.

[0065] Experimental Example 2

[0066] Safety test of moxa sticks:

[0067] (1) Burn the moxa sticks prepared in Example 1. After drying the Cambridge filter at room temperature for 24 hours, collect the smoke from the burning moxa sticks in Example 1 using a portable PM2.5 / pM10 sampling head. After drying for 24 hours, weigh the smoke and then dissolve the Cambridge filter with dimethyl sulfoxide to prepare a moxa smoke solution with a concentration of 15 mg / mL. After filtration and sterilization, dispense the solution into containers and store at -80℃ for later use.

[0068] (2) Add the tartaric acid solution prepared in step (1) to DMEM / F12 medium (containing 10% FBS, 100U / mL penicillin and 100μg / mL streptomycin) and dilute it 500 times to prepare DMEM / F12 medium containing tartaric acid solution.

[0069] (3) Mouse fibroblasts were collected and cultured in DMEM / F12 medium containing 10% FBS, 100 U / mL penicillin, and 100 μg / mL streptomycin. The culture was placed in a 37°C, 5% CO2 cell culture incubator. When the mouse fibroblasts reached 70% confluence, they were digested with 0.25% trypsin, centrifuged at 2000 rpm for 10 min, resuspended and counted, and the cell density was adjusted to 5 × 10⁴ cells / mL. 100 μL of the cells were seeded into 96-well plates.

[0070] (4) Remove the original culture medium from the 96-well plate and add 100 μL of the DMEM / F12 medium prepared in step (2) to each well. The control group is treated with DMEM / F12 medium containing 10% FBS, 100 U / mL penicillin, and 100 μg / mL streptomycin. Continue culturing for 24 hours and observe cell morphology under a microscope. The results are as follows: Figure 1 , Figure 2 As shown.

[0071] The results are as follows Figure 1 , Figure 2 The image shown is a microscopic morphological image of fibroblasts under different culture conditions. Figure 1 , Figure 2It can be seen that the cell morphology of Example 1 is similar to that of the control group, which is the normal morphology of mouse fibroblasts, and no damage was caused to the cell morphology. This indicates that the moxa stick prepared in Example 1 is safe.

[0072] In summary, the moxa stick prepared by this invention allows the medicinal powder to adhere firmly to the moxa wool, overcoming the shortcomings of easy powder detachment during combustion and uneven release of the effective components of traditional Chinese medicine. It also maintains a stable columnar shape. Furthermore, the process is simple, the conditions are mild, and the moxa stick is safe when fully burned.

[0073] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. Based on the present invention, some modifications or substitutions can be made, but these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of protection claimed by the present invention.

Claims

1. A preparation method of a traditional Chinese medicine moxa stick for consolidating kidney and cultivating primordial qi, characterized in that, The preparation method comprises the following steps: (1) taking the moxa dispersed in water, and performing ultrasonic treatment, then filtering and drying, to obtain a prepared product; (2) mixing the traditional Chinese medicine ingredients, i.e., Herba Cistanche, Herba Epimedii, Radix Astragali, Rhizoma Chuanxiong and Radix Angelicae Sinensis, crushing and sieving to obtain a traditional Chinese medicine powder; the weight proportions of the traditional Chinese medicine ingredients are as follows: 8-12 parts of Herba Cistanche, 5-10 parts of Herba Epimedii, 5-10 parts of Radix Astragali, 3-5 parts of Rhizoma Chuanxiong and 1-4 parts of Radix Angelicae Sinensis; (3) mixing the modified pullulan and the Broussonetia papyrifera bark powder to obtain an adhesive; the mass ratio of the modified pullulan to the Broussonetia papyrifera bark powder is 1: (1-3); the preparation process of the modified pullulan is as follows: mixing a pullulan aqueous solution with dilute sulfuric acid, then adding a sodium periodate aqueous solution, stirring in the dark, removing the sodium periodate, and freeze-drying to obtain oxidized pullulan; dissolving the oxidized pullulan in anhydrous ethanol, then adding theanine and mixing to perform a reaction, to obtain the modified pullulan; (4) adding deionized water to the adhesive obtained in step (3), and then adding the traditional Chinese medicine powder obtained in step (2) and mixing uniformly to obtain a mixture; (5) adding the moxa to the mixture obtained in step (4), mixing uniformly, and then compressing and drying to obtain moxa sticks.

2. The preparation method of the kidney-nourishing and vital essence-boosting Chinese medicine moxa stick according to claim 1, characterized in that, The concentration of the dilute sulfuric acid is 0.08-0.12 mol / L; the molar ratio of the pullulan to the sodium periodate is 1: (1-2); the stirring time in the dark is 4-6 h; the freeze-drying time is 48-72 h; the molar ratio of the oxidized pullulan to theanine is 1: (1.1-1.5); the reaction temperature after adding theanine and mixing is 60-80 ℃, and the reaction time is 2-4 h.

3. The preparation method of the kidney-nourishing and vital essence-boosting Chinese medicine moxa stick according to claim 1, characterized in that, In step (1), the ultrasonic treatment has a frequency of 20-50 KHZ, a power of 400-600 W, and a time of 1-2 h.

4. The preparation method of the kidney-nourishing and vital essence-boosting Chinese medicine moxa stick according to claim 1, characterized in that, In step (4), the mass ratio of the adhesive to the deionized water is 1: (1-3).

5. The preparation method of the kidney-nourishing and vital essence-boosting Chinese medicine moxa stick according to claim 1, characterized in that, In step (5), the drying is normal pressure drying, and the temperature is 60-80 ℃.

6. A traditional Chinese medicine moxa stick for consolidating kidney and cultivating primordial Qi, characterized in that, The moxa sticks are prepared by the preparation method according to any one of claims 1-5. The moxa sticks are prepared by the preparation method according to any one of claims 1-5.

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