Tendon-dredging and bone-penetrating health-care liquid and preparation method thereof

Abstract

The invention discloses a tendon-dredging and bone-penetrating health-care liquid and a preparation method thereof. According to the preparation, erycibe obtusifolia benth and radix aconiti preparata are taken as cores, the compatibility design of detoxification of liquorice and synergism of cassia twig is combined with a beta-cyclodextrin inclusion technology, so that the content of aconitine is smaller than or equal to 0.02 mg / mL, and the transdermal rate of ligustrazine is larger than or equal to 65%. The preparation process comprises three modules of dynamic circulation extraction, membrane separation refining and sterile filling, so that the retention rate of volatile components is greater than 85%. Animal experiments prove that the onset time of the analgesic effect is shortened by 40% compared with that of similar products, and the traditional Chinese medicine composition is suitable for conditioning sub-health states such as joint flexion and extension disadvantage.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicine liquid preparations, in particular to a bone-penetrating health-care liquid that achieves safety and high efficiency through toxic component control technology and a targeted transdermal system. Background Art

[0002] The existing technology has the following defects:

[0003] 1. Preparations containing aconite have poor safety.

[0004] 2. The transdermal retention rate of liquid topical medications is low (literature reports that the 24-hour permeation of eugenol in vitro is less than 40%). 3. Volatile components are easily oxidized and inactivated. Summary of the Invention

[0005] Innovative technical solutions:

[0006] 1. Toxicity reduction and efficacy enhancement balance system:

[0007] After detoxification by licorice, the aconitine content in processed Chuanwu decreased by 82.5% (LC-MS verification).

[0008] Cinnamaldehyde in cinnamon twig forms ion pairs with processed aconite alkaloids, increasing the transdermal rate by 3.2 times. 2. Stability control technology:

[0009] β-cyclodextrin inclusion increased the retention rate of costunolide from 54% to 91%

[0010] The nitrogen filling process makes the liquid oxygen content ≤0.5ppm. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 This is a process flow chart of a muscle-opening and bone-penetrating health liquid. DETAILED DESCRIPTION

[0012] Embodiment 1 (preferred embodiment):

[0013] Raw material ratio: Dinggongteng 10%, Chuanwu 2% (detoxified by licorice), Qianghuo 5%, Cinnamomum cassia twig 8%, potassium sorbate 0.2%

[0014] Key process parameters:

[0015] The cyclic decoction temperature was 83±1℃, and each extraction time was 1.5h.

[0016] The ceramic membrane filtration pressure is 0.3MPa and the flow rate is 8L / min.

[0017] The feed ratio of β-cyclodextrin to volatile oil is 1:4, and the inclusion time is 2h.

[0018] Quality testing methods:

[0019] 1. Aconitine limit test:

[0020] HPLC conditions: C18 column, acetonitrile-0.1% triethylamine (35:65), detection wavelength 235 nm

[0021] Retention time: 12.3 min, theoretical plate number > 5000.

[0022] 2. Microbial control:

[0023] The total number of aerobic bacteria is ≤50CFU / mL, and the total number of molds and yeasts is ≤10CFU / mL (membrane filtration method).

Claims

1. A muscle-opening and bone-penetrating health-care liquid, characterized in that Made from the following raw materials in weight percentage: Dinggongteng 5-15%, Notopterygium wilfordii 3-8%, processed Chuanwu 1-3%, Radix Trichosanthis 4-10%, Cinnamomum cassia twig 6-12%, Ligusticum chuanxiong 8-15%, Magnolia officinalis 2-5%, Cyperus rotundus 3-6%, Aucklandia lappa 2-4%, Fructus aurantii 5-10%, Paeonia suffruticosa 4-8%, Black Tiger 6-12%, Potassium sorbate 0.1-0.3%, and the balance is purified water; The health care liquid meets the following indicators: Aconitine content ≤ 0.02 mg / mL (HPLC detection) Transdermal absorption rate ≥65% (Franz diffusion cell method, 24h) The microbial limits meet the standards for external liquid preparations in the 2020 edition of the Chinese Pharmacopoeia.

2. The health care liquid according to claim 1, characterized in that: The processed Chuanwu is steamed under high pressure (121° C., 30 min) and is synergistically enhanced with cassia twig in a ratio of (1:2) to (1:4).

3. The health care liquid according to claim 1, characterized in that: The method comprises a volatile component inclusion process, using beta-cyclodextrin to molecularly encapsulate the volatile oils of costus root and cyperus rotundus, with an inclusion rate of ≥85%.

4. A method for preparing the health care liquid according to claim 1, characterized in that The following steps are involved: (1) Toxic component pretreatment: crushed Radix Aconiti Lateralis Preparata and azeotroped with liquorice decoction (1:5) for 30 minutes; (2) Dynamic extraction: Boil Dinggongteng, Qianjinba and Black Tiger at 80-85℃ for 3 times (add 8 times, 6 times and 4 times the amount of water respectively) The volatile components (Aucklandia lappa and Cyperus rotundus) were extracted into aromatic water by steam distillation; (3) Purification and concentration: The combined extracts were filtered through a ceramic membrane (molecular weight cut-off 10 kDa), and concentrated under reduced pressure to a relative density of 1.10-1.15; (4) Sterilization and filling: Add potassium sorbate, filter through a 0.22 μm microporous membrane, and fill with nitrogen.

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