A sterile operating table capable of effectively controlling the culture of in vitro isolated organs

Abstract

The present invention belongs to the technical field of in vitro organ culture, and provides a sterile operating table that can effectively control the culture of in vitro organs. The table includes a glass cover having a sterile cavity for effectively controlling the culture of in vitro organs, and the glass cover is made of a transparent material; at least one pair of gloves arranged on the glass cover; a micro-heating device, which is arranged on the outer wall of the top of the glass cover in a flat manner; a shower moistening device, which is arranged on the top of the sterile cavity; a condensing device, one part of which is externally arranged on the side wall of the glass cover and the other part is arranged in the sterile cavity; and a double-gate type taking and delivering device, which is externally arranged on the side wall of the glass cover. Compared with the existing technology, the table can effectively provide a suitable culture environment for in vitro organs, perform various operations on the cultured in vitro organs under sterile conditions, such as massage and biopsy, to improve the success rate and safety of the culture of in vitro organs; facilitate intuitive observation of the condition of the in vitro organs; and provide convenience for the operator's operation.

Description

Technical Field

[0001] The present invention belongs to the technical field of in vitro organ culture, and more specifically, relates to a sterile operating table capable of effectively controlling the culture of in vitro organs. Background Art

[0002] Organ culture, a broad form of tissue culture, involves isolating and culturing a portion of a living organism. Partial or entire organs are cultured without damaging their normal structure, preserving their three-dimensional structure and mimicking organ function under various conditions. Currently, in vitro organ culture requires a sterile environment to prevent infection.

[0003] In the prior art, for example, a utility model patent authorized in China discloses an organoid biological tissue culture device with publication number CN217230771U, which specifically discloses that "it includes a frame, a turntable mechanism and a liquid injection mechanism, wherein the turntable mechanism includes a circular tray and a drive assembly, and the liquid injection mechanism includes a lifting assembly and at least one set of liquid guide rods. The circular tray of the utility model is provided with slots adapted to the culture dish at equal angles, and the drive assembly can drive the circular tray to rotate intermittently, so that the circular tray drives the culture dish to rotate directly under the liquid guide rod." Although this existing patented technology can add a fixed amount of culture fluid or cover oil to the culture dish through the liquid guide rod, thereby realizing batch addition of culture fluid or cover oil for organoid biological tissue, thereby achieving a technical effect of not only improving accuracy but also higher efficiency; however, the entire device of this solution is exposed to the air and cannot be operated aseptically in a sterile environment, which leads to a low success rate and low safety in controlling the culture of in vitro organs.

[0004] To this end, the present invention provides a sterile operating table that can effectively control the culture of in vitro organs. Summary of the Invention

[0005] In view of the above problems existing in the existing technology, the purpose of the present invention is to provide a sterile operating table that can effectively control the culture of in vitro organs, which has the characteristics of improving the success rate and safety of culturing in vitro organs.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A sterile operating table capable of effectively controlling and culturing in vitro isolated organs, the sterile operating table comprising: a glass cover having a sterile cavity for placing the effectively controlled in vitro isolated organs, the glass cover being made of a transparent material for isolating the outside and forming a sterile environment through physical and chemical conditions;

[0008] At least one pair of gloves provided on the glass cover, allowing an operator to put on the gloves and enter the sterile cavity from the outside to operate on the ex vivo organ;

[0009] A micro-heating device is arranged on the top outer wall of the glass cover in a flat manner;

[0010] A shower moistening device, which is arranged on the top of the sterile cavity and is used to keep the ex vivo organ moist;

[0011] A condensation device, one portion of which is externally mounted on the side wall of the glass cover and the other portion of which is disposed in the sterile chamber, the condensation device being used to condense, collect, and remove water generated by evaporation from the isolated organ;

[0012] The double-gate pick-up and delivery device is placed on the side wall of the glass cover and is used to transfer internal and external items under sterile conditions.

[0013] As a further preferred technical solution of the present invention, it also includes an anti-fog film, wherein the glass cover includes a circular or square base cover body and a corner cover body with an angular roof slope;

[0014] The anti-fog film is adhered to the cavity wall of the corner cover body in an inner covering manner.

[0015] As a further preferred technical solution of the present invention, the condensing device includes at least one condensing sheet externally located on the side wall of the glass cover, at least one ramp plate and at least one water guide groove connected to the water inlet of the water pump externally located in the sterile chamber through a pipe, the ramp plate is arranged on the inner cavity wall of the glass cover below the condensing sheet, the water guide groove is arranged at the bottom of the glass cover, and one side of the opening on the water guide groove is docked with the lower side of the ramp plate.

[0016] As a further preferred technical solution of the present invention, there are two condensing plates, two inclined plates, and two water guide grooves;

[0017] The two condensing sheets are symmetrically arranged on the outer wall of the glass cover, the two slope plates are symmetrically arranged on the inner wall of the glass cover, and the two water guide grooves are arranged along the sides parallel to the width direction of the glass cover.

[0018] As a further preferred technical solution of the present invention, it also includes an organ placement table arranged in the sterile cavity, the organ placement table is composed of a layer of film material and has a net bag-like structure. The organ placement table is used to support the ex vivo organ.

[0019] As a further preferred technical solution of the present invention, it further comprises a tool storage table for placing specific biopsy puncture tools, and the tool storage table is arranged in the sterile cavity.

[0020] As a further preferred technical solution of the present invention, it further includes a temperature monitoring controller for monitoring the temperature value in the sterile chamber, and the temperature monitoring controller is fixed in the sterile chamber; or is externally placed on the glass cover.

[0021] As a further preferred technical solution of the present invention, the double-gate type pick-up and delivery device includes a double-opening sliding door, which has an inner door slidably embedded in the outer wall of the glass cover and an outer door arranged to slide relative to the inner door.

[0022] As a further preferred technical solution of the present invention, there are two pairs of gloves, and both pairs of gloves are made of latex or rubber material.

[0023] As a further preferred technical solution of the present invention, it further comprises an additional device for fixing the gloves, and the additional device is arranged on the side wall of the glass cover.

[0024] As described above, the present invention has the following beneficial effects:

[0025] The present invention utilizes the above-mentioned sterile operating table that can effectively control the culture of in vitro organs. Compared with the existing technology, by arranging a micro-heating device, a shower moistening device, a condensing device, and a double-gate pick-up and delivery device, it can effectively provide a suitable culture environment for in vitro organs, so that various operations such as massage and biopsy can be performed on the controlled culture of in vitro organs under sterile conditions, thereby improving the success rate and safety of in vitro organ culture; at the same time, the glass cover is made of transparent material, which facilitates intuitive observation of the condition of the in vitro organs; and the design of the entire scheme can provide convenience for the operator's operation.

[0026] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0028] Figure 1 A stereoscopic diagram of a sterile operating table capable of effectively controlling the culture of in vitro organs applied for by the present invention;

[0029] Figure 2 This is a schematic structural diagram of an aseptic operating table including additional devices for effectively controlling the culture of in vitro organs applied for by the present invention.

[0030] Summary of reference numerals and their descriptions:

[0031] 1. Water guide groove; 2. Inclined plate; 3. Condensation plate; 4. Anti-fog film; 5. Organ placement table; 6. Tool storage table; 7. Temperature monitoring controller; 8. Shower wetting device; 9. Double-gate delivery device;

[0032] 10. Glass cover; 100. Base cover; 110. Corner cover; 11. Sterile cavity; 12. Gloves. DETAILED DESCRIPTION

[0033] The following describes the implementation of the present invention through specific embodiments. People skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0034] It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the present invention. Therefore, they have no substantial technical significance. Any modification of the structure, change in the proportional relationship or adjustment of the size should still fall within the scope of the technical content disclosed by the present invention without affecting the efficacy and purpose that can be achieved by the present invention. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description and are not used to limit the scope of the implementation of the present invention. Changes or adjustments in their relative relationships should also be regarded as the scope of the implementation of the present invention without substantially changing the technical content. The specific structure can be described with reference to the drawings of the patent application.

[0035] Example 1

[0036] The present invention provides a sterile operating table that can effectively control the culture of in vitro organs. Figure 1 and Figure 2 As shown, it includes: a glass cover 10, having a sterile cavity 11 for placing an effectively controlled cultured in vitro organ, the glass cover 10 is made of a transparent material, and is used to isolate the outside and form a sterile environment through physical and chemical conditions;

[0037] At least one pair of gloves 12 is provided on the glass cover 10, allowing an operator to put on the gloves 12 and enter the sterile cavity 11 from the outside to operate on the external organ;

[0038] A micro-heating device (not shown in the figure, referred to below) is arranged on the top outer wall of the glass cover 10 in a flat manner;

[0039] a shower moistening device 8, which is arranged at the top of the sterile chamber and is used to keep the isolated organ moist;

[0040] A condensation device, one portion of which is externally disposed on the side wall of the glass cover 10 and the other portion of which is disposed in the sterile chamber 11, the condensation device being used to condense, collect, and remove water generated by evaporation of the isolated organ;

[0041] The double-gate type pick-up and delivery device 9 is externally arranged on the side wall of the glass cover 10 and is used to transfer internal and external objects under sterile conditions.

[0042] Example 2

[0043] Based on Example 1, please refer to Figure 1 or Figure 2 As shown, an anti-fog film 13 is also included, and the glass cover 10 includes a circular or square base cover body 100 and an angle cover body 110 with an angular roof slope;

[0044] The anti-fog film 13 is adhered to the cavity wall of the corner cover body 110 in an inner covering manner. The anti-fog film 4 combined with the setting of the micro-heating device can achieve drying of the area inside the sterile cavity 11 and prevent condensed water from flowing back to contact the external organ and causing contamination.

[0045] Example 3

[0046] Based on Example 2, please refer to Figure 1 and Figure 2 As shown, the condensing device includes at least one condensing sheet 3 externally mounted on the side wall of the glass cover, at least one inclined plate 2, and at least one water guide groove 1 connected to the water inlet of a water pump externally mounted on the sterile chamber 11 via a pipe. The inclined plate 2 is mounted on the inner wall of the glass cover 10 below the condensing sheet 3. The water guide groove 1 is located at the bottom of the glass cover 10, with one side of the opening of the water guide groove 1 abutting against the lower side of the inclined plate 2. This design reduces the local temperature of the sterile chamber 11 by 2-3°C, achieving steam enrichment and condensation, and realizing the dehumidification function of the device without causing severe airflow disturbances.

[0047] In this example, see Figure 1 As shown, there are two condensing sheets 3, two ramp plates 2 and two water guide grooves 1;

[0048] The two condensing sheets 3 are symmetrically arranged on the outer wall of the glass cover 10 , the two slope plates 2 are symmetrically arranged on the inner wall of the glass cover 10 , and the two water guide grooves 1 are arranged along the sides parallel to the width direction of the glass cover 10 .

[0049] See also Figure 1As shown, in this embodiment, an organ placement table 5 is further provided within the sterile chamber 11. The organ placement table 5 is constructed of a thin film material and has a net-like structure. The organ placement table 5 is used to support an isolated organ outside the body. This structure of the organ placement table 5 prevents damage to the isolated organ due to excessive deformation.

[0050] See also Figure 1 As shown, in this embodiment, a tool storage table 6 for placing specific biopsy puncture tools is also included, and the tool storage table 6 is arranged in the sterile cavity 11. It is convenient to place the biopsy puncture tools, and it is easy to take, put and use.

[0051] Example 4

[0052] Based on Example 1, please refer to Figure 1 and Figure 2 As shown, the apparatus further includes a temperature monitoring controller 7 for monitoring the temperature value in the sterile chamber 11. The temperature monitoring controller 7 is fixed in the sterile chamber 11 or externally mounted on the glass cover 10. By providing the temperature monitoring controller 7, it is convenient to monitor the temperature in the sterile chamber 11, timely understand and grasp the temperature changes in the sterile chamber 11, indicate the direction for subsequent temperature control, and ensure that the temperature in the sterile chamber 11 remains constant.

[0053] Example 5

[0054] Based on Example 1, please refer to Figure 1 and Figure 2 As shown, the double-gate type pickup and delivery device 9 includes a double-opening sliding rail door, which has an inner door slidably embedded in the outer wall of the glass cover and an outer door that slides relative to the inner door. In specific operation, when surgical instruments and other items need to be delivered into the sterile cavity 11 of the glass cover 10, the operator first opens the double-opening sliding rail door on the outside, puts the items in, and then closes the outer door. The items will be sterilized in the glass cover 10 of the double-gate type pickup and delivery device. After completion, the inner door can be manually opened by wearing rubber (or latex) gloves 12 inside the glass cover 10 to remove the items. Similarly, the operation of taking out tissue specimens from the inside to the outside for testing is similar to the above description.

[0055] Specifically, in this embodiment, there are two pairs of gloves 12, and both pairs of gloves 12 are made of latex or rubber.

[0056] In this example, see Figure 1 and Figure 2As shown, it also includes an additional device for fixing the gloves 12, and the additional device is arranged on the side wall of the glass cover 10. By arranging the additional device, it is convenient for the operator to achieve isolated aseptic operation during surgical detection, massage, etc.

[0057] When using the sterile operating table of the present invention that can effectively control the culture of in vitro organs, please refer to Figure 1 and Figure 2 Please refer to the following steps for operation.

[0058] Step 1, preparation: First, ensure that all devices have been cleaned and disinfected in advance, including the glass cover 10, gloves 12, condensation device, organ placement table 5, tool storage table 6, temperature monitoring controller 7, shower moistening device 8, and double-gate type transfer device 9. Then, place the in vitro organ on the organ placement table 5, which is made of a special film material to form a net-like structure to support the in vitro organ and prevent it from excessive deformation.

[0059] Step 2: Condensate treatment: Start the water pump, and connect the water inlet of the water pump to one end of the water guide groove 1 through a pipe, so as to ensure that the condensed water in the water guide groove 1 can be discharged in time, and prevent the accumulation of condensed water from affecting the sterile environment in the sterile chamber;

[0060] Step 3: Temperature control: The temperature inside the glass cover 10 is set by the temperature monitoring controller 7 to maintain the physiological activity of the isolated organ. Then, the micro-heating device is activated to achieve regional drying through the anti-fog film provided inside the corner cover body 110 with the angled roof slope, thereby preventing condensation water from flowing back and contaminating the isolated organ.

[0061] Step 4: condenser sheet operation: start the external condenser sheet 3 to reduce the temperature of the condenser sheet 3 by 2-3°C to achieve steam enrichment and condensation in the sterile chamber 11 while avoiding severe airflow disturbance;

[0062] Step 5: Use of operating gloves: The operator puts on the double-sided gloves 12 on the glass cover 10 to perform sterile massage and biopsy of the isolated organ in vitro;

[0063] Step 6: Biopsy sampling: Use the biopsy puncture tool placed on the tool storage table 6 to take samples, ensuring the sterility of the sampling process;

[0064] Step 7: Temperature monitoring and adjustment: Regularly monitor the temperature in the sterile chamber 11 through the temperature monitoring controller 7 and adjust it when necessary to maintain a constant storage temperature;

[0065] Step 8: Organ moistening: Then start the shower moistening device 8 to periodically supply sterile physiological saline to the organ through a shower nozzle with controllable flow rate to keep the organ moist in vitro;

[0066] Step 9. Sterile entry and exit of articles and specimens: Use a double-gate pick-up and delivery device to imitate the entry and exit method of personnel in a high-level biosafety laboratory to achieve sterile entry and exit of articles and specimens; specifically, the operator first opens the outer double-opening sliding door, puts the article in, and then closes the outer door of the double-opening sliding door. The article will be sterilized in the sterile cavity 11; after completion, the operator manually opens the inner door of the double-opening sliding door with rubber gloves 12 and takes out the article. The operation method of taking out tissue specimens and other test objects from the inside out is similar to the above, and will not be repeated here. Therefore, by providing a micro-heating device, a shower moistening device 8, a condensing device, and a double-gate pick-up and delivery device 9, a suitable culture environment can be effectively provided for the in vitro organ, so that various operations such as massage and biopsy can be performed on the in vitro organ under sterile conditions, thereby improving the success rate and safety of in vitro organ culture. At the same time, the glass cover 10 is made of a transparent material to facilitate intuitive observation of the condition of the in vitro organ. The design of the entire solution can provide convenience for the operator.

[0067] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be included therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

Claims

1. A sterile operating table capable of effectively controlling the culture of in vitro organs, characterized by: The aseptic operating table includes: a glass cover having a sterile cavity for placing an effectively controlled cultured in vitro organ, the glass cover being made of a transparent material and used to isolate the outside and form a sterile environment through physical and chemical conditions; At least one pair of gloves provided on the glass cover, allowing an operator to put on the gloves and enter the sterile cavity from the outside to operate on the ex vivo organ; A micro-heating device is arranged on the top outer wall of the glass cover in a flat manner; A shower moistening device, which is arranged on the top of the sterile cavity and is used to keep the ex vivo organ moist; A condensation device, one portion of which is externally mounted on the side wall of the glass cover and the other portion of which is disposed in the sterile chamber, the condensation device being used to condense, collect, and remove water generated by evaporation from the isolated organ; A double-gate pick-up and delivery device, which is externally mounted on the side wall of the glass cover and is used to transfer items inside and outside under sterile conditions; it also includes an anti-fog film, the glass cover includes a circular or square base cover body and a corner cover body with an angular roof slope; the anti-fog film is adhered to the cavity wall of the corner cover body in an inner covering manner; The condensing device includes at least one condensing sheet externally located on the side wall of the glass cover, at least one ramp plate, and at least one water guide groove connected to the water inlet of the water pump externally located in the sterile chamber via a pipe. The ramp plate is disposed on the inner wall of the glass cover below the condensing sheet, and the water guide groove is disposed at the bottom of the glass cover, with one side of the upper opening of the water guide groove being arranged in abutment with the lower side of the ramp plate. There are two condensing sheets, two ramp plates, and two water guide grooves. The two condensing sheets are symmetrically arranged on the outer wall of the glass cover, the two inclined plates are symmetrically arranged on the inner wall of the glass cover, and the two water guide grooves are arranged along the sides parallel to the width direction of the glass cover; the double-gate type picking and delivering device includes a double-opening sliding rail door, and the double-opening sliding rail door has an inner door slidably embedded in the outer wall of the glass cover and an outer door arranged to slide relative to the inner door.

2. The sterile operating table capable of effectively controlling the culture of in vitro organs according to claim 1, characterized in that: It also includes an organ placement table arranged in the sterile cavity. The organ placement table is made of a layer of film material and has a net bag-like structure. The organ placement table is used to support the ex vivo organ.

3. The sterile operating table capable of effectively controlling the culture of in vitro organs according to claim 1, characterized in that: It also includes a tool storage table for placing biopsy puncture tools, and the tool storage table is arranged in the sterile cavity.

4. The sterile operating table capable of effectively controlling the culture of in vitro organs according to claim 1, characterized in that: It also includes a temperature monitoring controller for monitoring the temperature value in the sterile cavity. The temperature monitoring controller is fixed in the sterile cavity; or is externally placed on the glass cover.

5. A sterile operating table capable of effectively controlling the culture of in vitro organs according to any one of claims 1 to 4, characterized in that: There are two pairs of gloves, and both pairs of gloves are made of latex or rubber material.

6. A sterile operating table capable of effectively controlling the culture of in vitro organs according to any one of claims 1 to 4, characterized in that: It also includes an additional device for fixing the gloves, and the additional device is arranged on the side wall of the glass cover.

Images