High-resolution synchronous imaging device for plant tissues and containing crystals

Abstract

The invention is applicable to the technical field of plant imaging devices, and provides a plant tissue and crystal-containing high-resolution synchronous imaging device which comprises a base, and a microscope module is mounted on the base; the microscope further comprises an auxiliary imaging module, the auxiliary imaging module comprises a mounting seat mounted on the microscope module, a rotating seat is rotatably mounted on the mounting seat, an adjusting knob used for driving the rotating seat to rotate is further arranged on the mounting seat, an optical fiber is arranged on the rotating seat, and the adjusting knob is connected with the optical fiber. The optical fiber is arranged between the common light source of the microscope module and the objective table, and the optical fiber is connected with the full-spectrum external optical fiber light source. The device ensures clear imaging of plant tissues around the crystal under the condition of ensuring clear imaging of the crystal, can realize synchronous and high-definition imaging and observation or photographing of the plant tissues and the crystal therein, and does not increase too much extra manufacturing cost.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant imaging devices, and in particular relates to a high-resolution synchronous imaging device for plant tissues and crystals contained therein. Background Art

[0002] Research in plant science, medicinal plants, Chinese herbal medicines, and plant ecology often involves dissecting plant parts and then conducting microscopic or ultrastructural observations of the crystal particles within. This allows for investigation of the biological functions of different types of crystals in plant tissues, or for the regulation of crystal formation for applications such as detoxification of traditional Chinese medicines. Therefore, accurate observation of crystals in plant tissues requires high-definition display, which is crucial for ensuring the scientific validity of research results.

[0003] Since the crystal particles in plant tissues are easily confused with the cell granular structures such as organelles and inclusions in tissue cells in terms of morphology, a technology is needed to achieve high-definition display of both the crystal particles and the tissue structure around the crystals. In this way, the position of the crystal can be clearly located, and the morphological and structural characteristics of the crystal and its surrounding tissues can be clearly observed, providing convenient conditions for scientific research.

[0004] Currently, in addition to traditional microscopes, researchers are using darkfield microscopes, polarized light microscopes, and electron microscopes to address scientific issues related to crystal research, but these studies have proven unsatisfactory. While darkfield and polarized light microscopes can clearly visualize crystal particles, the background is very dim, making it difficult to visualize the crystals simultaneously with their high-definition counterparts, resulting in "black and white" imaging. Electron microscopes are expensive, and while they offer superior magnification, their black-and-white images allow them to distinguish crystals from surrounding plant tissues primarily through morphology, similar to conventional optical microscopes. Summary of the Invention

[0005] The purpose of the embodiments of the present invention is to provide a high-resolution synchronous imaging device for plant tissue and crystals contained therein, aiming to solve the problems raised in the above-mentioned background technology.

[0006] The embodiment of the present invention is implemented as follows: a high-resolution synchronous imaging device for plant tissue and contained crystals, comprising a base on which a microscope module is mounted; and further comprising: An auxiliary imaging module includes a mounting base mounted on the microscope module, a rotating base rotatably mounted on the mounting base, and an adjustment knob for driving the rotating base to rotate. An optical fiber is provided on the rotating base, and the optical fiber is provided between the ordinary light source of the microscope module and the stage, and the optical fiber is connected to the full-spectrum external optical fiber light source.

[0007] A further technical solution is that the microscope module includes an arm and a common light source installed on the base, an eyepiece and an objective lens are provided on the top of the arm, a stage is provided in the middle of the arm, a fixing clip is installed on the upper surface of the stage, a light hole is also opened on the stage, and the common light source is located directly below the light hole.

[0008] Another object of an embodiment of the present invention is to provide a method for high-resolution synchronous imaging of plant tissue and contained crystals, based on the above-mentioned high-resolution synchronous imaging device for plant tissue and contained crystals, comprising the following steps: Step 1: Prepare a full-spectrum external fiber optic light source and turn it on so that the light is close to the brightness of an ordinary microscope. Then, turn the adjustment knob to rotate the rotating base to adjust the angle of the fiber optic. Observe the changes in the crystal's luminescence characteristics as the angle changes from 0 to 90 degrees until you find a position where the crystal brightness is relatively high and maintain this angle unchanged. Step 2: Adjust the light intensity of the optical fiber until the crystal particles are clearly visible and can be imaged in high definition. Keep the light intensity constant. At this time, high-definition imaging of the crystals and surrounding plant tissues is achieved simultaneously.

[0009] An embodiment of the present invention provides a high-resolution synchronous imaging device for plant tissues and crystals contained therein, which has practical significance in the accurate identification of plant materials with crystal particles when actually used. While ensuring clear imaging of crystals, clear imaging of plant tissues surrounding the crystals is also ensured, and plant tissues and crystals therein can be synchronously and clearly imaged and observed or photographed without increasing costs too much. The device provides a highly accurate research technology for realizing the automatic identification of plant materials based on crystals as the main feature, which can greatly improve the scientific research efficiency of such research. At the same time, the observation of crystals and surrounding tissues by the naked eye ensures the scientific nature of the research because crystals and surrounding tissues have completely different optical imaging characteristics. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 A schematic structural diagram of a high-resolution synchronous imaging device for plant tissue and contained crystals provided by an embodiment of the present invention; Figure 2 A schematic structural diagram of another perspective of a high-resolution synchronous imaging device for plant tissue and contained crystals provided by an embodiment of the present invention; Figure 3 for Figure 2 A magnified view of point A in the figure; Figure 4 The imaging effect of a high-resolution synchronous imaging device for plant tissue and contained crystals provided by an embodiment of the present invention; Figure 5The software for automatic identification and statistical analysis of the plant tissue crystal imaging method using a common optical microscope method and the present invention is used.

[0011] In the accompanying drawings: base 1; support arm 2; eyepiece 3; objective lens 4; stage 5; fixing clamp 51; light hole 52; ordinary light source 6; adjustment knob 7; mounting base 8; rotating base 9; optical fiber 10. DETAILED DESCRIPTION

[0012] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0013] The specific implementation of the present invention is described in detail below with reference to specific embodiments.

[0014] like Figure 1-Figure 3 As shown, a high-resolution synchronous imaging device for plant tissue and contained crystals provided by one embodiment of the present invention includes a base 1 on which a microscope module is mounted; and further includes: An auxiliary imaging module includes a mounting base 8 mounted on the microscope module, a rotating base 9 is rotatably mounted on the mounting base 8, and an adjustment knob 7 is also provided on the mounting base 8 for driving the rotating base 9 to rotate. An optical fiber 10 is provided on the rotating base 9, and the optical fiber 10 is arranged between the ordinary light source 6 of the microscope module and the stage 5, and the optical fiber 10 is connected to the full-spectrum external optical fiber light source.

[0015] In an embodiment of the present invention, when in use, first connect the full-spectrum external fiber optic light source to the optical fiber 10, and turn on the switch to make it emit light close to the original brightness of the microscope module, then turn the adjustment knob 7 to drive the rotating seat 9 to rotate, thereby adjusting the angle of the optical fiber 10. Observe the changes in the luminescence characteristics of the crystal during the angle change from 0 to 90 degrees, until the position where the crystal brightness is relatively high is found, and keep the angle unchanged. Adjust the light intensity of the optical fiber 10 until the crystal particles are clearly visible and can be imaged in high definition, and keep the light intensity unchanged. At this time, simultaneous high-definition imaging of the crystal and the surrounding plant tissue is achieved, and you can observe and take pictures at the same time. Images taken using this device are as follows: Figure 4 shown.

[0016] like Figure 1-Figure 3As shown in FIG. , as a preferred embodiment of the present invention, the microscope module can be a conventional microscope in the prior art. Specifically, the microscope module includes an arm 2 mounted on a base 1 and a conventional light source 6. An eyepiece 3 and an objective lens 4 are disposed on the top of the arm 2. A stage 5 is disposed in the middle of the arm 2. A fixing clamp 51 is mounted on the upper surface of the stage 5. A light hole 52 is also formed on the stage 5, and the conventional light source 6 is located directly below the light hole 52.

[0017] In the embodiment of the present invention, when using the microscope, one only needs to place the slide on the light hole 52 on the stage 5 and fix it with the fixing clip 51. Then, adjust the eyepiece 3 and the objective lens 4 to the desired magnification. At the same time, adjust the brightness of the ordinary light source 6 to obtain a clear image.

[0018] As a preferred embodiment of the present invention, the field images obtained by the device were tested using the automatic recognition software Image-plus. The test results showed that the automatic recognition statistical analysis results of the ordinary optical microscope plant tissue crystal images using Image-Plus 5.0 software were 16 times more than the actual number of crystals, and the automatic statistical analysis of the software was impossible ( Figure 5 Left). Figure 5 The picture on the right shows plant tissue crystals displayed in high definition in the same field of view. The error in the statistical analysis results of the software's automatic identification of crystals is very small, only 0.1 times less than the actual number of crystals. This provides a highly accurate research technology for the automatic identification of plant materials based on crystals as the main feature, which can greatly improve the scientific research efficiency of such studies. At the same time, the observation of the crystals and surrounding tissues by the naked eye with this technology ensures the scientific nature of the research because crystals and surrounding tissues have completely different optical imaging characteristics.

[0019] Another embodiment of the present invention provides a method for high-resolution synchronous imaging of plant tissue and contained crystals, based on the above-mentioned high-resolution synchronous imaging device for plant tissue and contained crystals, comprising the following steps: Step 1: Prepare a full-spectrum external fiber optic light source and turn it on so that the light is close to the brightness of an ordinary microscope. Then, turn the adjustment knob 7 to rotate the rotating base 9 to adjust the angle of the optical fiber 10. Observe the changes in the crystal's luminescence characteristics as the angle changes from 0 to 90 degrees until you find a position where the crystal brightness is relatively high, and maintain this angle unchanged. Step 2: Adjust the light intensity of the optical fiber 10 until the crystal particles are clearly visible and can be imaged in high definition. Keep the light intensity constant. At this time, high-definition imaging of the crystal and the surrounding plant tissue is achieved simultaneously.

[0020] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A high-resolution synchronous imaging device for plant tissue and contained crystals, comprising a base on which a microscope module is mounted, characterized in that: Also includes: An auxiliary imaging module includes a mounting base mounted on the microscope module, a rotating base rotatably mounted on the mounting base, and an adjustment knob for driving the rotating base to rotate. An optical fiber is provided on the rotating base, and the optical fiber is provided between the ordinary light source of the microscope module and the stage, and the optical fiber is connected to the full-spectrum external optical fiber light source.

2. The high-resolution synchronous imaging device for plant tissue and contained crystals according to claim 1, characterized in that: The microscope module includes an arm mounted on a base and a common light source. An eyepiece and an objective lens are provided on the top of the arm. A stage is provided in the middle of the arm. A fixing clip is installed on the upper surface of the stage. A light hole is also provided on the stage, and the common light source is located directly below the light hole.

3. A method for high-resolution synchronous imaging of plant tissue and contained crystals, based on the high-resolution synchronous imaging device for plant tissue and contained crystals according to any one of claims 1 and 2, characterized in that: The following steps are involved: Step 1: Prepare a full-spectrum external fiber optic light source and turn it on so that the light is close to the brightness of an ordinary microscope. Then, turn the adjustment knob to rotate the rotating seat to adjust the angle of the fiber optic. Observe the changes in the crystal's luminous properties as the angle changes from 0 to 90 degrees until you find a position where the crystal's brightness is relatively high, and keep that angle unchanged. Step 2: Adjust the light intensity of the optical fiber until the crystal particles are clearly visible and can be imaged in high definition. Keep the light intensity constant. At this time, high-definition imaging of the crystals and surrounding plant tissues is achieved simultaneously.

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