Method for testing dynamic wrinkle resisting effect of cosmetics and application of method

Abstract

The invention relates to a method for testing the dynamic wrinkle resisting effect of cosmetics and application of the method. The testing method comprises the following steps that (1) preset testing indexes are measured on the left face and the right face of a subject respectively; (2) the subject smiles to the maximum extent, fishtails and periorbital fine wrinkles are squeezed out, and according to the use requirements of the to-be-detected cosmetics, the to-be-detected cosmetics are used on the face on one side, and the to-be-detected cosmetics are not used on the face on the other side; (3) after using the product for 3-10 minutes, forcibly blinking the two eyes of the subject for 20-40 times at the same time; (4) respectively measuring preset test indexes on the left face and the right face of the subject; and (5) counting test results, and performing data analysis to obtain the dynamic wrinkle resistance effect of the to-be-tested cosmetic. The method can be used for testing whether the cosmetic to be tested has the effect of preventing and slowing down the generation of dynamic wrinkles, shortens the anti-wrinkle effect detection period, and reduces the time cost and the detection cost.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics testing, and in particular to a method for testing the anti-dynamic wrinkle effect of cosmetics and an application thereof. Background Art

[0002] Cosmetics are common daily products applied to the skin, hair, and nails by rubbing, spraying, or other methods. They are primarily used as industrial chemical products for cosmetic modification, beautification, cleansing, and protection. To evaluate the effectiveness of cosmetic products in reducing or preventing wrinkles, anti-wrinkle efficacy testing is required. Common testing methods include in vitro experiments, clinical trials, and subjective consumer evaluations.

[0003] In vitro testing methods include applying cosmetic ingredients to skin cells cultured in vitro, evaluating the product's anti-aging effects on cells by detecting the expression levels of components such as collagen and elastin in the cells, and using microscopes or biochemical analysis instruments to detect changes in cell morphology and composition. In addition, a three-dimensional skin imaging system is used to perform high-resolution imaging of the subject's face. Images are captured before and after the application of cosmetics, and changes in skin texture are measured using image analysis software. A detailed questionnaire can also be designed, including subjective evaluations of the product's effects, such as skin smoothness, firmness, and reduction in fine lines and wrinkles. Subjects fill out questionnaires before and after using cosmetics to record their feelings and experiences. The questionnaire data is then statistically analyzed to evaluate the product's anti-wrinkle effects.

[0004] For example, CN110161027A discloses a cosmetics efficacy evaluation method based on image analysis. An image acquisition device captures facial images before and after cosmetics application. An image analysis and calculation device then analyzes and compares facial skin parameters before and after cosmetics application to evaluate the cosmetics' efficacy. The method provided by the present invention avoids direct contact with the skin, thus avoiding the negative effects of probe pressure contact.

[0005] In summary, there is currently no real-time anti-wrinkle efficacy testing method for cosmetics against dynamic wrinkles. Providing a new real-time testing method has become one of the urgent problems to be solved in this field. Summary of the Invention

[0006] To solve the above technical problems, the present invention provides a testing method for the anti-dynamic wrinkle effect of cosmetics and its application. By making exaggerated large movements and expressions in a short period of time, it is tested whether the cosmetics to be tested have the effect of preventing and slowing down the formation of dynamic wrinkles, filling the gap in the instant anti-wrinkle efficacy test of dynamic wrinkles and expression lines.

[0007] To achieve this object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a method for testing the anti-dynamic wrinkle effect of cosmetics, the testing method comprising the following steps:

[0009] (1) Measure the preset test indicators on the left and right faces of the subject respectively, and obtain the left face data T0 and the right face data T0' before the test;

[0010] (2) The subject smiles as wide as possible, squeezing out crow's feet and fine lines around the eyes. Then, according to the requirements for the cosmetics to be tested, the left side of the face is not covered with the cosmetics to be tested, while the right side of the face is covered with the cosmetics to be tested.

[0011] (3) After using the product for 3 to 10 minutes, the subject blinked forcefully 20 to 40 times with both eyes simultaneously;

[0012] (4) measuring the preset test indicators on the left and right faces of the subject respectively, and obtaining the left face data T1 and the right face data T1′ after the test;

[0013] (5) Calculate the test results, perform data analysis, and determine the anti-dynamic wrinkle effect of the tested cosmetics.

[0014] The specific values of the above 3 to 10 minutes may be 3 minutes, 4 minutes, 5 minutes, 6 minutes, 7 minutes, 8 minutes, 9 minutes or 10 minutes, etc.

[0015] The specific values of the above 20 to 40 times may be 20 times, 25 times, 28 times, 30 times, 32 times, 35 times or 40 times.

[0016] The present invention evaluates whether the cosmetics to be tested have the effect of preventing and alleviating the generation of dynamic lines by measuring the dynamic lines generated by the subjects before and after using the cosmetics to be tested. The two methods of generating facial dynamic lines have a synergistic effect.

[0017] Preferably, the cosmetics include creams, lotions, lotions or facial masks.

[0018] Preferably, the selection criteria for the subjects include: (a) healthy adults aged between 30 and 60 years old; (b) subjects with sagging faces and obvious wrinkles in the test area; (c) subjects without severe chronic wasting diseases (such as asthma, diabetes, etc.); (d) subjects who have not participated in other clinical trials in the past month; (e) subjects who can fully understand the trial process, voluntarily participate in the trial and sign a written informed consent.

[0019] Preferably, the test method further includes the following steps before step (1): the subject cleans the facial skin with a facial cleanser and sits quietly for at least 20 minutes in an environment with a temperature of 20-22° C. and a relative humidity of 40%-60%.

[0020] The specific values of the above-mentioned 20-22°C may be 20°C, 20.5°C, 21°C, 21.5°C or 22°C, etc.

[0021] The specific values of the above 40% to 60% may be 40%, 45%, 48%, 50%, 52%, 55% or 60%, etc.

[0022] Preferably, the preset test indicators include any one of the number of under-eye wrinkles, the area of under-eye wrinkles, the volume of under-eye wrinkles, the number of crow's feet, the area of crow's feet, the volume of crow's feet, the skin elasticity R2 value or the skin firmness F4 value, or a combination of at least two of them.

[0023] In the present invention, the lower the value of wrinkle number / area / volume, the smaller the wrinkle number / area / volume is, and the unit is dimensionless / mm 2 / mm 3 ; The higher the skin elasticity R2 value, the better the skin elasticity, and the unit is dimensionless; the lower the skin firmness F4 value, the better the skin firmness, and the unit is dimensionless.

[0024] Preferably, the preset test indicators are under-eye wrinkle area, under-eye wrinkle volume, crow's feet area, skin elasticity R2 value and skin firmness F4 value.

[0025] Preferably, the testing device for the number, area and volume of under-eye wrinkles, the number, area and volume of crow's feet wrinkles comprises Evaskin-S5.

[0026] Preferably, the testing equipment for the skin elasticity R2 value and the skin firmness F4 value includes Cutometer MPA580.

[0027] Preferably, the measurement comprises: acquiring Evaskin-S5 images and / or MPA580 probe data of the subject's face.

[0028] Preferably, the step of obtaining the anti-dynamic wrinkle effect of the cosmetic to be tested specifically includes: if at least three preset test indicators have significant differences before and after the test, then the cosmetic to be tested has the anti-dynamic wrinkle effect.

[0029] Preferably, step (5) of the testing method comprises:

[0030] (5-1) Calculate the median, maximum, minimum, standard deviation (SD), standard error (SE) and mean of T0, T1, T0' and T1';

[0031] (5-2) Calculate the difference and change rate between the blank group and the experimental group. The blank group difference △T1=T1 mean-T0 mean, and the experimental group difference △T1'=T1' mean-T0' mean.

[0032] Preferably, step (5) of the testing method further comprises:

[0033] (5-3) Determine whether △T1 and △T1' are normally distributed. If they are normally distributed, use the t-test method for statistical analysis. If they are not normally distributed, use the non-parametric test method for statistical analysis. The statistical method uses a two-tailed test with a test level of α = 0.05.

[0034] (5-4) Calculate whether the preset test indicators have significant differences before and after the test. If at least three preset test indicators have significant differences before and after the test, the cosmetic to be tested has an anti-dynamic wrinkle effect.

[0035] In a second aspect, the present invention provides application of the testing method described in the first aspect in screening cosmetic raw materials.

[0036] Other specific point values within the above numerical ranges can be selected and will not be described in detail here.

[0037] Compared with the prior art, the present invention has the following beneficial effects:

[0038] The present invention adds a new method for testing dynamic lines based on the cosmetic anti-wrinkle efficacy testing method. By making exaggerated large facial movements within a short period of time, then applying a sample, and then blinking multiple times, the test is conducted to determine whether the cosmetic to be tested has the effect of preventing or alleviating the formation of dynamic lines. This fills the gap in the instant anti-wrinkle efficacy testing of dynamic lines and expression lines, and also provides a new instant testing method for the anti-wrinkle efficacy testing of cosmetics. At the same time, it shortens the anti-wrinkle efficacy testing cycle, reducing time and testing costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is a graph showing the changing trend of the number of wrinkles under the eyes.

[0040] Figure 2 This is a graph showing the volume change trend of under-eye wrinkles.

[0041] Figure 3 This is a graph showing the changing trend of under-eye wrinkle area.

[0042] Figure 4 This is a graph showing the changing trend of the number of crow's feet wrinkles.

[0043] Figure 5 This is a graph showing the volume change trend of crow's feet wrinkles.

[0044] Figure 6 This is a trend chart of changes in the area of crow's feet wrinkles.

[0045] Figure 7 This is a trend chart of skin elasticity R2 value changes.

[0046] Figure 8 This is a graph showing the changing trend of skin firmness F4 value. DETAILED DESCRIPTION

[0047] To further illustrate the technical means and effects of the present invention, the present invention is further described below with reference to the embodiments and drawings. It should be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention.

[0048] If no specific techniques or conditions are specified in the examples, the experiments were carried out according to the techniques or conditions described in the literature in the field or according to the product instructions. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0049] The instruments used in the following examples are:

[0050] Skin elasticity tester Cutometer MPA580 (Courage & Khazaka, Germany).

[0051] Skin fast optical imaging system Evaskin-S5 (EOTECH, France).

[0052] Example 1

[0053] This embodiment provides a method for testing the anti-dynamic wrinkle effect of cosmetics, the testing method comprising the following steps:

[0054] S1. Subjects were selected based on the following criteria: (a) healthy adults aged between 30 and 60 years; (b) subjects with sagging facial features and obvious wrinkles in the test area; (c) subjects without severe chronic wasting diseases (such as asthma, diabetes, etc.); (d) subjects who had not participated in other clinical trials in the past month; and (e) subjects who could fully understand the trial process, voluntarily participated in the trial and signed a written informed consent.

[0055] S2. Subjects visited the hospital. Subjects who met the inclusion criteria were enrolled and signed informed consent. They cleaned their facial skin with facial cleanser and sat quietly in an environment of 21±1°C and 50±10% RH for at least 20 minutes.

[0056] S3. Laboratory technicians used the Evaskin-S5 and MPA580 instruments to measure preset test indicators on the left and right sides of the subjects' faces, respectively, obtaining pre-test left facial data T0 (hereinafter referred to as the blank group T0) and pre-test right facial data T0' (hereinafter referred to as the experimental group T0').

[0057] S4. Under the guidance of the laboratory technician, the subject smiled as wide as possible, exposing the crow's feet and fine lines around the eyes. Then, according to the instructions for the cosmetics to be tested, the left side of the face was not used, and the right side of the face was used for the cosmetics to be tested.

[0058] S5. After using the product for 5 minutes, the subject blinked forcefully 30 times with both eyes at the same time.

[0059] S6. Laboratory technicians operate the corresponding instruments to measure the preset test indicators on the left and right sides of the subject's face, respectively, and obtain the left side face data T1 (hereinafter referred to as the blank group T1) and the right side face data T1' (hereinafter referred to as the blank group T1') after the test.

[0060] S7. Use data analysis software such as EXCEL to perform statistics and analyze the test results to determine the anti-dynamic wrinkle effect of the tested cosmetics, including:

[0061] S7-1. Calculate the median, maximum, minimum, standard deviation (SD), standard error (SE), and mean of T0, T1, T0', and T1'.

[0062] S7-2. Calculate the difference and rate of change between the blank group and the test group. The blank group difference △T1 = T1 mean - T0 mean, the test group difference △T1' = T1' mean - T0' mean;

[0063] S7-3. Determine whether △T1 and △T1' are normally distributed. If they are normally distributed, use the t-test method for statistical analysis. If they are non-normally distributed, use the non-parametric test method for statistical analysis. The statistical method uses a two-tailed test with an α test level of 0.05.

[0064] S7-4. Calculate whether there is a significant difference in the preset test indicators before and after the test. If there is a significant difference in at least three preset test indicators before and after the test, the cosmetic to be tested has an anti-dynamic wrinkle effect.

[0065] The preset test indicators include the number of under-eye wrinkles, the area of under-eye wrinkles, the volume of under-eye wrinkles, the number of crow's feet, the area of crow's feet, the volume of crow's feet, the skin elasticity R2 value and the skin firmness F4 value; the testing equipment for the number of under-eye wrinkles, the area of under-eye wrinkles, the volume of under-eye wrinkles, the number of crow's feet, the area of crow's feet and the volume of crow's feet is Evaskin-S5; the testing equipment for the skin elasticity R2 value and the skin firmness F4 value is Cutometer MPA580.

[0066] Test Example 1

[0067] This test example uses the test method provided in Example 1 to test whether the cosmetics to be tested have anti-dynamic wrinkle effects. The usage requirements of the cosmetics to be tested are: take an appropriate amount of product in the palm of your hand, apply it evenly on the facial skin, and massage and lift until it is completely absorbed.

[0068] The statistical results of the number of under-eye wrinkles are shown in Table 1, the changes in the number of under-eye wrinkles are shown in Table 2, and the difference test results between the two groups are shown in Table 3. Figure 1 As shown, * / ** / *** / ns represent p<0.05 / p<0.01 / p<0.001 / not significant, respectively. The test results showed that after using the tested cosmetics, the number of under-eye wrinkles decreased significantly by 13.08% compared to before use, and there was a significant difference compared to the blank control group.

[0069] Table 1

[0070] Number of wrinkles under the eyes Blank group T0 Blank group T1 Experimental group T0' Experimental group T1' median 26.00 26.00 26.00 21.50 Maximum 36.00 41.00 34.00 31.00 Minimum 15.00 14.00 17.00 15.00 Standard deviation SD value 4.37 5.43 4.55 5.08 Standard error SE value 0.77 0.96 0.80 0.90 mean 26.63 26.03 25.56 22.22

[0071] Table 2

[0072] Number of wrinkles under the eyes △Blank group T1 △Experimental group T1' Difference -0.59 -3.34 Rate of change / % -2.23 -13.08 Normality test p-value 0.198 0.302 Is it normally distributed? yes yes Inspection methods Paired t-test Paired t-test Significance p-value 0.482 0.001 Is it significant Not significant significant

[0073] Table 3

[0074]

[0075] The statistical results of under-eye wrinkle volume are shown in Table 4, the results of under-eye wrinkle volume change are shown in Table 5, and the difference test results between the two groups are shown in Table 6. Figure 2 As shown, * / ** / *** / ns represent p<0.05 / p<0.01 / p<0.001 / not significant, respectively. The test results showed that after using the tested cosmetics, the volume of under-eye wrinkles decreased by 16.90% compared to before use, and there was a significant difference compared to the blank control group.

[0076] Table 4

[0077] Volume of under-eye wrinkles Blank group T0 Blank group T1 Experimental group T0' Experimental group T1' median 0.9 0.86 0.80 0.66 Maximum 3.12 2.92 1.78 1.86 Minimum 0.37 0.40 0.41 0.26 Standard deviation SD value 0.51 0.48 0.39 0.43 Standard error SE value 0.09 0.09 0.07 0.08 mean 1.03 0.97 0.96 0.79

[0078] Table 5

[0079] Volume of under-eye wrinkles △Blank group T1 △Experimental group T1' Difference -0.05 -0.16 Rate of change / % -5.14 -16.90 Normality test p-value 0.911 0.201 Is it normally distributed? yes yes Inspection methods Paired t-test Paired t-test Significance p-value 0.020 0.001 Is it significant significant significant

[0080] Table 6

[0081]

[0082]

[0083] The statistical results of under-eye wrinkle area are shown in Table 7, the results of under-eye wrinkle area change are shown in Table 8, and the difference test results between the two groups are shown in Table 9. Figure 3 As shown, * / ** / *** / ns represent p<0.05 / p<0.01 / p<0.001 / not significant, respectively. The test results show that after using the tested cosmetics, the area of under-eye wrinkles decreased significantly by 15.72% compared to before use, and there was a significant difference compared to the blank control group.

[0084] Table 7

[0085] Area of wrinkles under the eyes Blank group T0 Blank group T1 Experimental group T0' Experimental group T1' median 26.04 24.24 24.23 19.96 Maximum 47.91 46.00 38.46 37.08 Minimum 14.87 12.50 14.71 7.54 Standard deviation SD value 7.97 7.84 6.58 7.75 Standard error SE value 1.41 1.39 1.16 1.37 mean 26.57 25.06 25.04 21.10

[0086] Table 8

[0087]

[0088]

[0089] Table 9

[0090]

[0091] The statistical results of the number of crow's feet wrinkles are shown in Table 10, the changes in the number of crow's feet wrinkles are shown in Table 11, and the difference test results between the two groups are shown in Table 12. Figure 4 As shown, * / ** / *** / ns represent p<0.05 / p<0.01 / p<0.001 / not significant, respectively. The test results show that after using the tested cosmetics, the number of crow's feet wrinkles decreased significantly by 14.43% compared to before use, and there was a significant difference compared to the blank control group.

[0092] Table 10

[0093] Number of crow's feet wrinkles Blank group T0 Blank group T1 Experimental group T0' Experimental group T1' median 24.00 23.00 25.50 21.00 Maximum 34.00 33.00 32.00 33.00 Minimum 15.00 16.00 15.00 12.00 Standard deviation SD value 4.82 4.39 4.35 4.82 Standard error SE value 0.85 0.78 0.77 0.85 mean 24.63 23.56 25.34 21.69

[0094] Table 11

[0095]

[0096]

[0097] Table 12

[0098]

[0099] The statistical results of crow's feet wrinkle volume are shown in Table 13, the results of crow's feet wrinkle volume change are shown in Table 14, and the difference test results between the two groups are shown in Table 15. Figure 5As shown, * / ** / *** / ns represent p<0.05 / p<0.01 / p<0.001 / not significant, respectively. The test results show that after using the tested cosmetics, the volume of crow's feet wrinkles decreased significantly by 14.87% compared to before use, and there was a significant difference compared to the blank control group.

[0100] Table 13

[0101]

[0102]

[0103] Table 14

[0104] Crow's feet wrinkle volume △Blank group T1 △Experimental group T1' Difference -0.03 -0.13 Rate of change / % -4.54 -14.87 Normality test p-value 0.002 0.000 Is it normally distributed? no no Inspection methods Signed rank test Signed rank test Significance p-value 0.089 0.000 Is it significant Not significant significant

[0105] Table 15

[0106]

[0107] The statistical results of crow's feet wrinkle area are shown in Table 16, the results of crow's feet wrinkle area change are shown in Table 17, and the difference test results between the two groups are shown in Table 18. Figure 6 As shown, * / ** / *** / ns represent p<0.05 / p<0.01 / p<0.001 / not significant, respectively. The test results show that after using the tested cosmetics, the area of crow's feet wrinkles decreased by 15.20% compared to before use, and there was a significant difference compared to the blank control group.

[0108] Table 16

[0109]

[0110]

[0111] Table 17

[0112] Crow's feet wrinkle area △Blank group T1 △Experimental group T1' Difference -1.23 -3.54 Rate of change / % -5.42 -15.20 Normality test p-value 0.002 0.002 Is it normally distributed? no no Inspection methods Signed rank test Signed rank test Significance p-value 0.002 0.000 Is it significant significant significant

[0113]

[0114]

[0115] The statistical results of skin elasticity R2 values are shown in Table 19, the changes of skin elasticity R2 values are shown in Table 20, and the difference test results between the two groups are shown in Table 21. Figure 7 As shown, * / ** / *** / ns represent p<0.05 / p<0.01 / p<0.001 / not significant, respectively. The test results show that after using the tested cosmetics, the R2 value of skin elasticity increased significantly by 19.21% compared to before use, and there was a significant difference compared to the blank control group.

[0116] Table 19

[0117] Skin elasticity R2 value Blank group T0 Blank group T1 Experimental group T0' Experimental group T1' median 55.70 56.55 51.20 60.10 Maximum 73.60 78.20 63.90 83.00 Minimum 34.20 43.00 31.70 46.50 Standard deviation SD value 9.69 8.12 8.65 7.95 Standard error SE value 1.71 1.44 1.53 1.41 mean 54.56 57.44 51.39 61.27

[0118] Table 20

[0119] Skin elasticity R2 value △Blank group T1 △Experimental group T1' Difference 2.88 9.87 Rate of change / % 5.28 19.21 Normality test p-value 0.283 0.098 Is it normally distributed? yes yes Inspection methods Paired t-test Paired t-test Significance p-value 0.010 0.000 Is it significant significant significant

[0120] Table 21

[0121]

[0122] The statistical results of skin firmness F4 value are shown in Table 22, the change results of skin firmness F4 value are shown in Table 23, and the difference test results between the two groups are shown in Table 24. Figure 8 As shown, * / ** / *** / ns represent p<0.05 / p<0.01 / p<0.001 / not significant, respectively. The test results show that after using the tested cosmetics, the skin firmness F4 value decreased significantly by 16.49% compared to before use, and there was a significant difference compared to the blank control group.

[0123] Table 22

[0124] Skin firmness F4 value Blank group T0 Blank group T1 Experimental group T0' Experimental group T1' median 2.72 2.45 3.47 2.68 Maximum 4.23 4.39 5.14 4.49 Minimum 0.88 0.83 1.83 1.18 Standard deviation SD value 0.82 0.89 0.94 0.90 Standard error SE value 0.15 0.16 0.17 0.16 mean 2.72 2.56 3.37 2.81

[0125] Table 23

[0126] Skin firmness F4 value △Blank group T1 △Experimental group T1' Difference -0.17 -0.56 Rate of change / % -6.07 -16.49 Normality test p-value 0.350 0.377 Is it normally distributed? yes yes Inspection methods Paired t-test Paired t-test Significance p-value 0.006 0.000 Is it significant significant significant

[0127] Table 24

[0128]

[0129] The above test results show that the number of wrinkles under the eyes decreased significantly by 13.08% compared with before use, the volume of wrinkles under the eyes decreased significantly by 16.90% compared with before use, the area of wrinkles under the eyes decreased significantly by 15.72% compared with before use, the number of crow's feet wrinkles decreased significantly by 14.43% compared with before use, the volume of crow's feet wrinkles decreased significantly by 14.87% compared with before use, the area of crow's feet wrinkles decreased significantly by 15.20% compared with before use, the skin elasticity R2 value increased significantly by 19.21% compared with before use, and the skin firmness F4 value decreased significantly by 16.49% compared with before use, and there were significant differences compared with the blank control group, indicating that the test product has potential anti-wrinkle effects.

[0130] In summary, the present invention adds a dynamic wrinkle testing method based on the cosmetic anti-wrinkle efficacy testing method, providing a new instant testing method for cosmetic anti-wrinkle efficacy testing, while shortening the anti-wrinkle efficacy testing cycle and reducing time and testing costs.

[0131] The applicant declares that the above is only a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention fall within the scope of protection and disclosure of the present invention.

Claims

1. A method for testing the anti-dynamic wrinkle effect of cosmetics, characterized in that: The test method comprises the following steps: (1) Measure the preset test indicators on the left and right faces of the subject respectively, and obtain the left face data T0 and the right face data T0' before the test; (2) The subject smiles as wide as possible, squeezing out crow's feet and fine lines around the eyes. Then, according to the requirements for the cosmetics to be tested, the left side of the face is not covered with the cosmetics to be tested, while the right side of the face is covered with the cosmetics to be tested. (3) After using the product for 3 to 10 minutes, the subject blinked forcefully 20 to 40 times with both eyes simultaneously; (4) measuring the preset test indicators on the left and right faces of the subject respectively, and obtaining the left face data T1 and the right face data T1′ after the test; (5) Calculate the test results, perform data analysis, and determine the anti-dynamic wrinkle effect of the tested cosmetics.

2. The testing method according to claim 1, wherein: The cosmetics include creams, lotions, lotions or facial masks; Preferably, the selection criteria for the subjects include: (a) healthy adults aged between 30 and 60 years old; (b) subjects with sagging faces and obvious wrinkles in the test area; (c) subjects without severe chronic wasting diseases; (d) subjects who have not participated in other clinical trials in the past month; (e) subjects who can fully understand the trial process, voluntarily participate in the trial and sign a written informed consent.

3. The testing method according to claim 1 or 2, characterized in that: The test method further includes the following steps before step (1): the subject cleans the facial skin with a facial cleanser and sits quietly for at least 20 minutes in an environment with a temperature of 20-22° C. and a relative humidity of 40%-60%.

4. The testing method according to any one of claims 1 to 3, characterized in that: The preset test indicators include any one or a combination of at least two of the following: the number of under-eye wrinkles, the area of under-eye wrinkles, the volume of under-eye wrinkles, the number of crow's feet wrinkles, the area of crow's feet wrinkles, the volume of crow's feet wrinkles, the skin elasticity R2 value or the skin firmness F4 value; Preferably, the preset test indicators are under-eye wrinkle area, under-eye wrinkle volume, crow's feet area, skin elasticity R2 value and skin firmness F4 value.

5. The testing method according to claim 4, characterized in that: The testing equipment for the number, area and volume of under-eye wrinkles, the number, area and volume of crow's feet wrinkles includes Evaskin-S5; Preferably, the testing equipment for the skin elasticity R2 value and the skin firmness F4 value comprises Cutometer MPA580.

6. The testing method according to any one of claims 1 to 5, characterized in that: The measurement includes: collecting Evaskin-S5 images and / or MPA580 probe data of the subject's face.

7. The testing method according to any one of claims 1 to 6, characterized in that: The method of determining the anti-dynamic wrinkle effect of the cosmetic to be tested specifically includes: if at least three preset test indicators have significant differences before and after the test, then the cosmetic to be tested has the anti-dynamic wrinkle effect.

8. The testing method according to any one of claims 1 to 7, characterized in that: Step (5) of the test method comprises: (5-1) Calculate the median, maximum, minimum, standard deviation (SD), standard error (SE) and mean of T0, T1, T0' and T1'; (5-2) Calculate the difference and change rate between the blank group and the experimental group. The blank group difference △T1=T1 mean-T0 mean, and the experimental group difference △T1'=T1' mean-T0' mean.

9. The testing method according to claim 8, characterized in that: Step (5) of the test method further comprises: (5-3) Determine whether △T1 and △T1' are normally distributed. If they are normally distributed, use the t-test method for statistical analysis. If they are not normally distributed, use the non-parametric test method for statistical analysis. The statistical method uses a two-tailed test with a test level of α = 0.

05. (5-4) Calculate whether the preset test indicators have significant differences before and after the test. If at least three preset test indicators have significant differences before and after the test, the cosmetic to be tested has an anti-dynamic wrinkle effect.

10. Use of the testing method according to any one of claims 1 to 9 in screening cosmetic raw materials.

Images