Transplanting device for Chinese orchid tissue culture seedlings

Abstract

The invention relates to the technical field of tissue culture seedling transplanting, in particular to a Chinese orchid tissue culture seedling transplanting device which comprises a conveying mechanism, a first mounting frame, a second mounting frame, a third mounting frame, a planting frame, a movable lifting mechanism and a moving-out mechanism. The movable lifting mechanism is arranged at the top end of the first mounting frame. According to the Chinese orchid tissue culture seedling transplanting device, mechanical damage to roots and bases of Chinese orchid tissue culture seedlings can be avoided, meanwhile, automatic transplanting of the Chinese orchid tissue culture seedlings is achieved, the survival rate of the transplanted Chinese orchid tissue culture seedlings is effectively guaranteed, the transplanting efficiency of the Chinese orchid tissue culture seedlings is improved, and after the transplanted Chinese orchid tissue culture seedlings are cleaned, the transplanting efficiency of the Chinese orchid tissue culture seedlings is improved. According to the Chinese orchid tissue culture seedling screening device, automatic screening of Chinese orchid tissue culture seedlings is achieved, abnormal tissue culture seedlings and tissue culture seedlings with unqualified root development are separated to the second screen, meanwhile, healthy tissue culture seedlings enter the material receiving box, and then the overall survival rate and the yield of Chinese orchid adult seedlings are effectively increased.

Description

Technical Field

[0001] The invention relates to the technical field of tissue culture seedling transplanting, in particular to a cymbidium tissue culture seedling transplanting device. Background Art

[0002] Cymbidium orchid, also known as Chinese orchid, is a general term for orchid plants native to China, Japan and parts of Southeast Asia. It is a treasure among traditional ornamental flowers. In the modern breeding of Cymbidium orchid, tissue culture seedling technology (plant tissue culture) is widely used to achieve efficient propagation and variety improvement. However, the survival rate of tissue culture seedlings is low after being transferred from a sterile environment to a natural cultivation environment. Therefore, transplanting of Cymbidium orchid tissue culture seedlings is very important.

[0003] At present, when transplanting tissue culture seedlings, the commonly used process is: first, the tissue culture seedlings are hardened, and then the staff manually scrapes the culture medium and grasps the base of the orchid to pull out the tissue culture seedlings, cleans the tissue culture seedlings, and finally transplants them into the planting rack. However, this process has significant disadvantages. On the one hand, the roots and bases of the tissue culture seedlings are easily mechanically damaged during the manual grasping and pulling process, which greatly affects the survival rate of the tissue culture seedlings after transplanting. On the other hand, this process lacks an effective screening mechanism for tissue culture seedlings, resulting in the mixing of substandard tissue culture seedlings into the planting process, thereby greatly reducing the overall survival rate and finished product rate of orchid seedlings, and is not practical. Summary of the Invention

[0004] The purpose of the present invention is to solve the problem in the existing technology that the tissue culture seedling transplanting process easily causes damage to the roots and bases of the tissue culture seedlings, and lacks an effective screening mechanism for the tissue culture seedlings, thereby reducing the overall survival rate and finished product rate of the tissue culture seedlings after transplanting and seedling formation.

[0005] In order to achieve the above-mentioned object, the present invention adopts the following technical scheme: a Chinese orchid tissue culture seedling transplanting device, comprising a conveying mechanism, a mounting frame 1, a mounting frame 2, a mounting frame 3 and a planting frame, wherein a tissue culture bottle is placed on the conveying mechanism, and a culture medium and tissue culture seedlings are placed in the tissue culture bottle, and further comprising:

[0006] A movable lifting mechanism and a removal mechanism, wherein the movable lifting mechanism is arranged at the top of the mounting frame, and the removal mechanism is arranged on the movable lifting mechanism and is located directly above the tissue culture bottle, and is used to remove the tissue culture seedlings in the tissue culture bottle and prevent the roots and bases of the tissue culture seedlings from being damaged;

[0007] The cleaning and screening mechanism includes a cleaning component and a screening component. The cleaning component includes a cleaning frame. An opening is provided at the top of the cleaning frame, and a feed port is provided at the outer wall near the removal mechanism. A liquid inlet pipe 2 is installed at the outer wall of the cleaning frame near the top position. A stirring component is provided on the opening. A soft brush is installed on the stirring component for causing the soft brush to revolve along the opening while rotating. The soft brush is located in the cleaning frame. A square frame opening is installed on the outer wall of the cleaning frame. A gate valve is installed on the square frame opening. The height of the square frame opening corresponds to the height of the tissue culture bottle. The width of the square frame opening is 1.2 times the conventional width of the tissue culture seedlings. The screening component is arranged on the mounting frame 3 and the cleaning component to cooperate with the cleaning component to effectively screen the cleaned tissue culture seedlings.

[0008] Preferably, a liquid inlet pipe 1 and a discharge pipe are respectively installed at the bottom end of the cleaning frame, a bubble generator is installed at the connection between the liquid inlet pipe 1 and the cleaning frame, a ball valve is installed on the discharge pipe, a screen 1 is installed near the bottom end of the cleaning frame, the bottom end of the soft brush is in contact with the top end of the screen 1, and a discharge trough arranged in a downward tilt is installed near the bottom end at the outward end of the square frame opening.

[0009] Preferably, the stirring component includes a mounting frame, which is fixedly mounted on the opening, and its top is connected to the mounting frame one, a motor three is mounted at the center position of the top of the mounting frame, and an inner gear ring is mounted on its inner wall, the output end of the motor three passes through the top wall of the mounting frame and is fixedly connected to a cross bar, a rotating frame is mounted on one end of the cross bar, and is rotatably connected to a gear through the rotating frame, the gear is meshed with the inner gear ring, and the top of the soft brush passes through the bottom wall of the rotating frame and is fixedly connected to the bottom end of the gear.

[0010] Preferably, the screening component includes a material collection frame, which is installed on the top of the mounting frame three and has a screen two installed inside. A material receiving box is installed on the top of the screen two. The material receiving box is located close to the end of the material collection frame away from the cleaning frame, and has multiple through holes on its inner bottom wall. The height of the discharge trough corresponds to the height of the culture medium in the tissue culture bottle, and through grooves are provided on the top wall and the inner bottom wall of the discharge trough. The width of the through grooves corresponds to the conventional root diameter of the tissue culture seedlings. The bottom wall of the outward end of the discharge trough is connected to the top wall of the material receiving box near the cleaning frame. A return pipe is installed at the bottom end of the material collection frame, and is connected to the discharge pipe through the return pipe.

[0011] Preferably, two baffles are symmetrically installed on the top of the aggregate frame, and the two baffles are both tilted outwards and are respectively located on both sides of the discharge chute.

[0012] Preferably, the conveying mechanism includes a conveyor belt, a bracket and a drive assembly. The bracket is arranged on the ground, the drive assembly is arranged at the top of the bracket, the conveyor belt is installed on the drive assembly, and a plurality of positioning grooves are opened on the conveyor belt, and the tissue culture bottles are placed in the positioning grooves. The mobile lifting mechanism includes a moving part and a mounting plate. The mounting plate is installed on the moving part. The moving part is arranged at the top of the mounting frame for enabling the mounting plate to be displaced. Two cylinders are installed at the top of the mounting plate. The output ends of the two cylinders both pass through the top wall of the mounting plate and are fixedly connected to the removal mechanism.

[0013] Preferably, the removal mechanism includes a fixed plate, which is mounted on the output ends of the two cylinders and has a motor 1 mounted on the top. A rotating rod is mounted on the output end of the motor 1. The bottom end of the rotating rod passes through the top wall of the fixed plate and is fixedly connected to the connecting plate. A plurality of connecting rods are evenly mounted on the bottom end of the connecting plate. The bottom ends of the plurality of connecting rods are commonly fixedly connected to a mounting ring. The bottom end of the mounting ring is tilted inward, and a groove is provided on the inclined surface of the inner wall thereof.

[0014] Four rotating cavities are evenly opened near the bottom end of the mounting ring, and a small gear is rotatably connected in the rotating cavity. The positions of the four small gears correspond to the positions of four connecting rods in the multiple connecting rods. A connecting rod is installed at the bottom end of the small gear, and the bottom end of the connecting rod passes through the bottom wall of the rotating cavity and the top wall of the groove in sequence, and is fixedly connected to a scraper. The scraper is tilted inward, and the connection between the connecting rod and the scraper is near the edge of one end of the scraper. The four scrapers are all located in the groove, and the side wall of the scraper close to the groove is in contact with the inner wall of the groove. A power assembly is provided on the connecting plate, the mounting ring and the multiple connecting rods for rotating the four small gears.

[0015] Preferably, the outer diameter of the connecting plate is the same as that of the mounting ring, and the outer diameters of the connecting plate and the mounting ring are 5 mm smaller than the inner diameter of the tissue culture bottle. The angle between the inward inclined surface of the scraper and the horizontal plane is 45 degrees.

[0016] Preferably, the power assembly includes a cavity, a rotating cavity 2 and a motor 2. The cavity and the rotating cavity 2 are both opened inside the mounting ring. The cavity is rotatably connected to an outer gear ring and is communicated with the four rotating cavity 1 interiors. The outer gear ring is engaged with the four pinion 1s. The rotating cavity 2 is rotatably connected to pinion 2 and is communicated with the interior of the cavity. The outer gear ring is engaged with pinion 2. Motor 2 is fixedly mounted on the top of the connecting plate, and its output end is fixedly connected to a drive rod. The bottom end of the drive rod passes through the connecting plate, the corresponding connecting rod, the top wall of the mounting ring and the top wall of the rotating cavity 2 in sequence, and is fixedly connected to the top of pinion 2.

[0017] Preferably, multiple piezoelectric ceramics are fixedly embedded on the side walls of the four scrapers away from the groove, and four wires are installed in the mounting ring. The positions of the four wires correspond to the positions of the four pinion gears respectively. The bottom ends of the wires pass through the corresponding top wall of the rotating cavity, pinion gear, connecting rod and scraper top wall in sequence, and are electrically connected to the multiple piezoelectric ceramics on the scraper, and the top ends of the wires pass through the inner wall of the mounting ring, the corresponding connecting rod and connecting plate in sequence, and are connected to the external power supply.

[0018] Compared with the existing technology, the advantages of the present invention are:

[0019] 1. The present invention cooperates with the mobile lifting mechanism, the removing mechanism and the cleaning and screening mechanism to not only avoid mechanical damage to the roots and bases of the Cymbidium tissue culture seedlings, but also realizes their automatic removal, thereby effectively ensuring the survival rate of the Cymbidium tissue culture seedlings after transplantation and improving the removal efficiency of the Cymbidium tissue culture seedlings. After cleaning the removed Cymbidium tissue culture seedlings, the opening of the gate valve, the counterclockwise rotation of the soft brush, the state of the tissue culture seedlings in sterile water, and the setting of the square frame mouth and the discharge chute can be used to realize automatic screening of the Cymbidium tissue culture seedlings, so that abnormal tissue culture seedlings and tissue culture seedlings with unqualified root development are separated onto the second screen, and at the same time, healthy tissue culture seedlings are allowed to enter the receiving box, thereby effectively improving the overall survival rate and finished product rate of the Cymbidium seedlings.

[0020] 2. The present invention arranges multiple piezoelectric ceramics on the scraper in the removal mechanism, and can use the vibration of the scraper to gradually break the culture medium on the cymbidium tissue culture seedlings and drop them into the tissue culture bottle when the cymbidium tissue culture seedlings are automatically removed, thereby reducing the culture medium carried by the roots of the cymbidium tissue culture seedlings and reducing the difficulty of subsequent cleaning.

[0021] 3. The present invention, through the arrangement of the cleaning component in the cleaning and screening mechanism, can utilize the CTAB solution and the bubbles generated by it in combination with the rotation and revolution of the soft-bristle brush to effectively brush off the culture medium and dirt attached to the tissue culture seedlings. At the same time, the negatively charged tissue culture seedlings adsorb the positively charged bubbles in the CTAB solution and the bubbles attached to the surface of the tissue culture seedlings are broken to peel off the neutral culture medium and dirt on the tissue culture seedlings, thereby enabling the soft-bristle brush to better brush off the culture medium and dirt attached to the tissue culture seedlings, effectively improving the cleaning efficiency and quality of the tissue culture seedlings. In addition, after the CTAB solution completes the cleaning work, the tissue culture seedlings can be brushed a second time by injecting sterile water in combination with the rotation and revolution of the soft-bristle brush to clean the residual CTAB solution and bubbles thereon.

[0022] 4. The present invention cooperates with the stirring component and the soft brush in the cleaning assembly, which can not only realize the brushing of tissue culture seedlings by the soft brush, but also can realize the brushing of screen 1 in the cleaning frame, thereby ensuring the cleanliness and smoothness of screen 1, preventing it from being blocked by the brushed culture medium and dirt, and effectively ensuring the smooth progress of the tissue culture seedling cleaning work. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a working schematic diagram of the Cymbidium tissue culture seedling transplanting device proposed by the present invention;

[0024] Figure 2 This is an axonometric diagram of the removal mechanism of the Cymbidium tissue culture seedling transplanting device proposed by the present invention;

[0025] Figure 3 It is a partial axonometric diagram of the removal mechanism of the Cymbidium tissue culture seedling transplanting device proposed by the present invention;

[0026] Figure 4 A semi-sectioned axonometric view of the removal mechanism of the tissue culture seedling transplanting device for Cymbidium orchids proposed by the present invention;

[0027] Figure 5 for Figure 4 A partial enlarged view of the middle X;

[0028] Figure 6 It is a partial cross-sectional isometric view of the removal mechanism of the tissue culture seedling transplanting device for Cymbidium orchids proposed in the present invention;

[0029] Figure 7 for Figure 6 A partial enlarged view of Y in the middle;

[0030] Figure 8 This is an axonometric diagram of the cleaning component portion of the Cymbidium tissue culture seedling transplanting device proposed by the present invention;

[0031] Figure 9 A half-section axonometric view of the cleaning component portion of the Cymbidium tissue culture seedling transplanting device proposed by the present invention;

[0032] Figure 10 This is a rear axonometric view of the cleaning component portion of the Cymbidium tissue culture seedling transplanting device proposed by the present invention;

[0033] Figure 11 This is a semi-sectioned axonometric view of the screening component portion of the Cymbidium tissue culture seedling transplanting device proposed in the present invention.

[0034] In the figure: 1. Conveying mechanism; 2. Tissue culture bottle; 3. Removal mechanism; 4. Cleaning assembly; 5. Screening assembly; 6. Planting rack; 11. Positioning slot; 31. Cylinder; 32. Moving part; 33. Mounting plate; 34. Motor 1; 35. Motor 2; 36. Connecting rod; 37. Scraper; 38. Pinion 1; 39. Pinion 2; 310. Wire; 311. Outer gear ring; 312. Piezoelectric ceramic; 41. Feed inlet; 42. Motor 3; 43. Liquid inlet pipe 1; 44. Bubble generator; 45. Liquid inlet pipe 2; 46. Discharge pipe; 47. Ball valve; 48. Gate valve; 49. Discharge chute; 410. Gear; 411. Soft brush; 412. Screen 1; 413. Through chute; 51. Return pipe; 52. Receiving box; 53. Screen 2. DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0036] Reference Figures 1 to 6 , the Chinese orchid tissue culture seedling transplanting device includes a conveying mechanism 1, a mounting frame 1, a mounting frame 2, a mounting frame 3 and a planting frame 6. A tissue culture bottle 2 is placed on the conveying mechanism 1, and a culture medium and a tissue culture seedling are placed in the tissue culture bottle 2. The conveying mechanism 1 is used to transport the tissue culture bottle 2 to a suitable position, and the conveying mechanism 1 includes a conveyor belt, a bracket and a drive assembly. The bracket is set on the ground, the drive assembly is set on the top of the bracket, the conveyor belt is installed on the drive assembly, and a plurality of positioning grooves 11 are opened on the conveyor belt. The tissue culture bottle 2 is placed in the positioning groove 11. The drive assembly is a prior art, and its specific structural design is not repeated here. The top of the mounting frame is provided with The mobile lifting mechanism is provided with a removing mechanism 3, which is located directly above the tissue culture bottle 2 and is used to take out the tissue culture seedlings in the tissue culture bottle 2 and prevent the roots and bases of the tissue culture seedlings from being damaged. The mobile lifting mechanism includes a moving part 32 and a mounting plate 33. The mounting plate 33 is installed on the moving part 32. The moving part 32 is arranged at the top of the mounting frame to enable the mounting plate 33 to be displaced. The moving part 32 is a prior art and its specific structural design is not repeated here. Two cylinders 31 are installed on the top of the mounting plate 33. The output ends of the two cylinders 31 both pass through the top wall of the mounting plate 33 and are fixedly connected to the removing mechanism 3.

[0037] Reference Figures 1 to 7The removing mechanism 3 includes a fixed plate, which is mounted on the output ends of the two cylinders 31, and a motor 34 is mounted on the top. A rotating rod is mounted on the output end of the motor 34, and the bottom end of the rotating rod passes through the top wall of the fixed plate and is fixedly connected to the connecting plate. A plurality of connecting rods 36 are evenly mounted on the bottom end of the connecting plate, and the bottom ends of the plurality of connecting rods 36 are commonly fixedly connected to a mounting ring. The outer diameter of the connecting plate is the same as the outer diameter of the mounting ring. The outer diameters of the connecting plate and the mounting ring are 5 mm smaller than the inner diameter of the tissue culture bottle 2. The bottom end of the mounting ring is tilted inward, and a groove is provided on the inclined surface of the inner wall. Four rotating cavities are evenly provided near the bottom end of the mounting ring. There is a rotating cavity 1 in the rotating cavity. Pinion 1 38, the positions of the four pinion 1 38 respectively correspond to the positions of the four connecting rods 36 in the multiple connecting rods 36, and a connecting rod is installed at the bottom end of the pinion 1 38. The bottom end of the connecting rod passes through the bottom wall of the rotating cavity 1 and the top wall of the groove in sequence, and is fixedly connected to a scraper 37. The scraper 37 is tilted inward, and the angle between the inward inclined surface and the horizontal plane is 45 degrees. The connection between the connecting rod and the scraper 37 is close to the edge of one end of the scraper 37. The four scrapers 37 are all located in the groove, and the side wall of the scraper 37 close to the groove is in contact with the inner wall of the groove. A power component is provided on the connecting plate, the mounting ring and the multiple connecting rods 36 to rotate the four pinion 1 38.

[0038] Reference Figures 3 to 7 The power assembly includes a cavity, a rotating cavity 2 and a motor 2 35. The cavity and the rotating cavity 2 are both opened inside the mounting ring. The cavity is rotatably connected to an outer gear ring 311 and is communicated with the four rotating cavity 1 interiors. The outer gear ring 311 is engaged with four pinion 1s 38. The rotating cavity 2 is rotatably connected to a pinion 2 39 and is communicated with the cavity interior. The outer gear ring 311 is engaged with the pinion 2 39. The motor 2 35 is fixedly mounted on the top of the connecting plate, and its output end is fixedly connected to a drive rod. The bottom end of the drive rod passes through the connecting plate, the corresponding connecting rod 36, the top wall of the mounting ring and the top wall of the rotating cavity 2 in sequence, and is fixedly connected to the top of the pinion 2 39. The four scrapers 37 are away from A plurality of piezoelectric ceramics 312 are fixedly embedded on one side wall of the groove, and four wires 310 are installed in the mounting ring. The positions of the four wires 310 correspond to the positions of the four pinion gears 38 respectively. The bottom end of the wire 310 passes through the corresponding top wall of the rotating cavity, the pinion gear 38, the connecting rod and the top wall of the scraper 37 in sequence, and is electrically connected to the multiple piezoelectric ceramics 312 on the scraper 37. The top end of the wire 310 passes through the inner wall of the mounting ring, the corresponding connecting rod 36 and the connecting plate in sequence, and is connected to the external power supply. The wire 310 is used to transmit the electrical energy of the external power supply to the multiple piezoelectric ceramics 312 on the scraper 37, thereby providing the necessary power supply for the piezoelectric ceramics 312.

[0039] Reference Figure 1 as well as Figures 8 to 10, a cleaning and screening mechanism is provided on the mounting frame 1, the mounting frame 2 and the mounting frame 3, and the cleaning and screening mechanism includes a cleaning component 4 and a screening component 5. The cleaning component 4 is used to clean the removed tissue culture seedlings, and the screening component 5 is used to cooperate with the cleaning component 4 to effectively screen the cleaned tissue culture seedlings, thereby ensuring the quality of the tissue culture seedlings transplanted to the planting rack 6. The cleaning component 4 includes a cleaning frame, the top of the cleaning frame is provided with an opening, and the outer wall is provided with a feed port 41 near the removal mechanism 3. The bottom end of the cleaning frame is respectively provided with a liquid inlet pipe 1 43 and a discharge pipe 46, and a liquid inlet pipe 2 45 is installed near the top position of the outer wall. A bubble generator 44 is installed at the connection between the liquid inlet pipe 1 43 and the cleaning frame, a ball valve 47 is installed on the discharge pipe 46, and a stirring component is provided on the opening. A soft brush 411 is installed on the stirring component, which is used to make the soft brush 411 rotate along the opening while realizing self-rotation. A screen 412 is installed near the bottom end of the cleaning frame. The soft brush 411 is located in the cleaning frame, and its bottom end is in contact with the top of the screen 412. The stirring component includes a mounting frame, which is fixedly mounted on the opening, and its top is connected to the mounting frame 1. A motor 3 42 is installed at the center position of the top of the mounting frame, and an inner gear ring is installed on the inner side wall thereof. The output end of the motor 3 42 passes through the top wall of the mounting frame and is fixedly connected to a cross bar. A rotating frame is installed at one end of the cross bar, and is rotatably connected to a gear 410 through the rotating frame. The gear 410 is meshed with the inner gear ring. The top of the soft brush 411 passes through the bottom wall of the rotating frame and is fixedly connected to the bottom end of the gear 410.

[0040] Reference Figures 8 to 11 , a square frame opening is installed on the outer wall of the cleaning frame, a gate valve 48 is installed on the square frame opening, and the height of the square frame opening corresponds to the height of the tissue culture bottle 2, the width of the square frame opening is 1.2 times the conventional width of the tissue culture seedling, and a discharge chute 49 is installed at the outward end near the bottom end of the square frame opening, and the screening component 5 includes an aggregate frame, the aggregate frame is installed at the top of the mounting frame three, and a screen 2 53 is installed inside, and a receiving box 52 is installed on the top of the screen 2 53, and the receiving box 52 is located near the end of the aggregate frame away from the cleaning frame, and the inner bottom wall thereof is penetrated It is provided with multiple through holes, the height of the discharge trough 49 corresponds to the height of the culture medium in the tissue culture bottle 2, and through grooves 413 are provided on the top wall and the bottom wall of the discharge trough 49. The width of the through groove 413 corresponds to the conventional root diameter of the tissue culture seedlings. The bottom wall of the outward end of the discharge trough 49 is connected to the top wall of the material receiving box 52 near the cleaning frame. A return pipe 51 is installed at the bottom end of the collection frame, and is connected to the discharge pipe 46 through the return pipe 51. Two baffles are symmetrically installed on the top of the collection frame. The two baffles are both inclined outward and are respectively located on both sides of the discharge trough 49.

[0041] In the present invention, after hardening, the Cymbidium tissue culture seedlings are placed together with the tissue culture bottle 2 in the positioning groove 11 on the conveyor belt, and the tissue culture bottle 2 is stepped and conveyed to the bottom of the removal mechanism 3 by the conveying mechanism 1. Then, the cylinder 31 is opened to move the removal mechanism 3 downward and insert it into the culture medium in the tissue culture bottle 2 until the bottom end of the mounting ring in the removal mechanism 3 contacts the inner bottom wall of the tissue culture bottle 2. Since the outer diameter of the connecting plate in the removal mechanism 3 is the same as the outer diameter of the mounting ring, and the outer diameters of the connecting plate and the mounting ring are both 5 mm smaller than the inner diameter of the tissue culture bottle 2 (that is, the outer diameters of the connecting plate and the mounting ring are slightly smaller than the inner diameter of the tissue culture bottle 2), when the mounting ring is inserted into the culture medium, it will not cause damage to the roots of the tissue culture seedlings in the culture medium.

[0042] When the bottom end of the mounting ring contacts the inner bottom wall of the tissue culture bottle 2, the motor 2 35 is started, thereby driving the driving rod and the small gear 2 39 at the bottom end of the driving rod to rotate. Since the small gear 2 39 is engaged with the outer gear ring 311, and the outer gear ring 311 is engaged with the four small gears 1 38, the rotation of the small gear 2 39 can drive the outer gear ring 311 and the four small gears 1 38 to rotate synchronously. Since the four small gears 1 38 are connected to the scraper 37 through the connecting rod at their bottom ends, and the connection between the connecting rod and the scraper 37 is close to the edge of one end of the scraper 37, when the four small gears 1 38 are rotated by controlling the motor 2 35, the four scrapers 37 can be rotated and flipped out from the groove until all four scrapers 37 are rotated. After 90 degrees, turn off motor 2 35 (at this time, the scraper 37 changes from the initial storage state to the unfolded state), and then start motor 1 34 to rotate the connecting plate, so that the connecting rod 36, the mounting ring and the scraper 37 on the mounting ring rotate. During the continuous rotation of the scraper 37, the culture medium and the tissue culture bottle 2 can be effectively separated. Since the angle between the inward inclined surface of the scraper 37 and the horizontal plane is 45 degrees, when the scraper 37 is performing a scraping operation and accidentally contacts the roots of the orchid, the roots can be pushed away to prevent the scraper 37 from cutting the roots of the orchid, that is, avoiding damage to the roots of the orchid, thereby ensuring the integrity of the orchid tissue culture seedlings while also ensuring the survival rate of the orchid tissue culture seedlings after transplantation.

[0043] Then, the cylinder 31 is used to move the removal mechanism 3 upward and reset. Since the scraper 37 is tilted inward, the removal mechanism 3 can move the Cymbidium tissue culture seedlings together with the culture medium upward and out of the tissue culture bottle 2 under the support of the scraper 37. During the upward movement of the removal mechanism 3, the external power supply is turned on to power the multiple piezoelectric ceramics 312 on the scraper 37, so that the piezoelectric ceramics 312 generate low-intensity ultrasonic vibrations and transmit the generated ultrasonic vibrations to the scraper 37. At this time, the scraper 37 will vibrate, and under the vibration of the scraper 37, the culture medium thereon will gradually break and fall into the tissue culture bottle 2, thereby reducing the culture medium carried by the roots of the Cymbidium tissue culture seedlings, so as to reduce the difficulty of subsequent cleaning. When the removal mechanism 3 is completely reset, the moving part 32 is controlled to The mounting plate 33 is moved toward the feed port 41 on the cleaning frame, thereby causing the removal mechanism 3 to move toward the feed port 41 on the cleaning frame until the removal mechanism 3 moves above the feed port 41. At this time, the scraper 37 is reset by controlling the motor 2 35, and the Cymbidium tissue culture seedlings that have lost their support will fall into the cleaning frame, thereby realizing the automated removal of the Cymbidium tissue culture seedlings. Compared with the conventional manual removal method, this automated process effectively avoids the problem of mechanical damage to the roots and base of the tissue culture seedlings during the process of manually scraping off the culture medium and grasping the base of the Cymbidium to pull out the tissue culture seedlings, and eliminates the uncertainty caused by differences in human operations, thereby effectively ensuring the survival rate of the Cymbidium tissue culture seedlings after transplantation, while improving the removal efficiency of the Cymbidium tissue culture seedlings and significantly reducing labor intensity.

[0044] Then, the above-mentioned operations of placing the tissue culture bottle 2 and removing the Cymbidium tissue culture seedlings and dropping them into the cleaning frame are repeated until a certain number of Cymbidium tissue culture seedlings are put into the cleaning frame. When the Cymbidium tissue culture seedlings enter the cleaning frame, they will naturally fall onto the screen 1 412. Subsequently, a low-concentration CTAB solution is injected into the cleaning frame through the liquid inlet pipe 1 43 and the bubble generator 44 is turned on. Under the action of the bubble generator 44, a large number of micro-nano bubbles will be generated in the CTAB solution entering the cleaning frame, that is, the CTAB solution entering the cleaning frame is in a gas-liquid mixed state. As the CTAB solution is continuously injected, the motor 3 42 is turned on to make the cross bar, the rotating frame and the soft brush 411 revolve clockwise along the opening on the cleaning frame (the rotation direction is Figure 8 As shown in the figure, the gear 410 on the rotating frame is meshed with the inner gear ring, and the soft brush 411 is fixedly connected to the bottom end of the gear 410. When the rotating frame revolves, the gear 410 will rotate, thereby driving the soft brush 411 to rotate. Under the synergistic effect of the revolution and rotation of the soft brush 411, on the one hand, the tissue culture seedlings can be separated and the culture medium and dirt attached to the tissue culture seedlings can be effectively brushed off. On the other hand, the screen 412 can be continuously brushed to ensure that the screen 412 is clean and unobstructed, and to prevent it from being blocked by the culture medium and dirt brushed off, thereby effectively ensuring the smooth progress of the cleaning work.

[0045] The surface of the tissue culture seedlings carries a negative charge due to their own structural characteristics. Specifically, the pectin groups in the cell wall will dissociate under specific conditions, releasing negatively charged ions. At the same time, the phosphate groups in the phospholipid bilayer of the cell membrane also give the cell membrane surface a negative charge. The culture medium (such as agar, sugar, etc.) remaining at the root of the tissue culture seedlings is usually neutral in the solution. At the same time, the CTAB solution is positively charged. Specifically, CTAB is a cationic surfactant. After dissolving in water, the cationic groups in its molecular structure will give the solution a positive charge. Therefore, the surface of the bubbles in the CTAB solution will be wrapped with positive charges. As the CTAB solution is continuously injected, the bubbles in the CTAB solution will adhere to the surface of the tissue culture seedlings and break. The impact force generated by the bubble breaking will peel off the neutral culture medium and dirt on the tissue culture seedlings, so that the soft-bristle brush 411 can better brush off the culture medium and dirt attached to the tissue culture seedlings, effectively improving the cleaning efficiency and quality of the tissue culture seedlings.

[0046] When the level of the CTAB solution in the cleaning frame approaches the feed port 41, the injection of the CTAB solution is stopped and after the soft brush 411 rotates for a period of time, the waste liquid in the cleaning frame together with the stripped culture medium and dirt is discharged through the discharge pipe 46 by opening the ball valve 47. Then, the ball valve 47 is closed and sterile water is injected into the cleaning frame through the liquid inlet pipe 2 45. As the sterile water is continuously injected, the soft brush 411 is rotated counterclockwise by controlling the motor 3 42 to scrub the tissue culture seedlings (the rotation direction is Figure 8 As shown in the figure, the roots of healthy seedlings in sterile water are naturally facing downward, while the roots of abnormal seedlings are facing upward or floating. During this process, since some bubbles attached to the surface of the tissue culture seedlings may not be broken, these unbroken bubbles will increase the buoyancy of the tissue culture seedlings, causing them to float or face upward. Therefore, the unbroken bubbles attached to the surface of the tissue culture seedlings can be broken by rotating the soft brush 411, so that the healthy tissue culture seedlings can maintain a stable state with their roots facing downward in the water.

[0047] When the sterile water level in the cleaning frame approaches the feed port 41, the gate valve 48 is opened (the square frame port begins to drain water at this time), and the water injection state of the liquid inlet pipe 2 45 and the rotation state of the soft brush 411 are maintained. Since the height of the square frame port corresponds to the height of the tissue culture bottle 2 and the width of the square frame port is 1.2 times the conventional width of the tissue culture seedling, the square frame port only allows one tissue culture seedling to pass through at a time. In addition, as shown in the attached Figure 8 As shown, when the soft brush 411 rotates counterclockwise and stirs the sterile water in the cleaning frame, the swirling direction of the sterile water corresponds to the direction of the outer end of the square frame opening. Therefore, through the swirling of the sterile water and the negative pressure of the drainage from the square frame opening, the tissue culture seedlings in the cleaning frame will be flushed into the discharge trough 49 in sequence.

[0048] Since the discharge chute 49 is installed at the outward end of the square frame near the bottom, and the height of the discharge chute 49 corresponds to the height of the culture medium in the tissue culture bottle 2 (the height of the culture medium in the tissue culture bottle 2 is equivalent to the height of the roots of the tissue culture seedlings in the tissue culture bottle 2), the roots of the tissue culture seedlings with their roots facing downward (i.e., healthy tissue culture seedlings) will enter the discharge chute 49 and slide down into the material receiving box 52. Since the width of the through groove 413 on the discharge chute 49 corresponds to the conventional root diameter of the tissue culture seedlings, and the root width of the conventional tissue culture seedlings is greater than the height of their base, the root width of the healthy tissue culture seedlings that rush into the discharge chute 49 is sufficient to support them to stay in the discharge chute 49, and they will not pass through the through groove 413 located below and fall onto the screen 2 53 in the collection frame. Tissue culture seedlings with unqualified root development will fall from the through groove 413 onto the screen 2 53 due to insufficient root width.

[0049] At the same time, if the tissue culture seedlings with their roots facing upwards (i.e., abnormal tissue culture seedlings) rush into the discharge chute 49, their bases will enter the discharge chute 49. At this time, the roots of the abnormal tissue culture seedlings will be stuck by the upper through grooves 413. Since their roots are located at the top and the weight of the roots of conventional tissue culture seedlings is greater than the weight of the base, the tissue culture seedlings will bend and deform under the action of gravity, thereby escaping from the discharge chute 49 and falling onto the screen 2 53 in the aggregate frame below. If the weight of the roots of the tissue culture seedlings is less than the weight of the base, making it difficult for them to bend and fall, it means that the roots of the tissue culture seedlings are poorly developed. At this time, the tissue culture seedlings will pass directly through the through grooves 413. It falls onto the second screen 53 through the through groove 413 on the discharge trough 49. In addition, since the floating tissue culture seedlings (i.e., abnormal tissue culture seedlings) are not in an upright state, they will not enter the discharge trough 49 when leaving the square frame mouth under the swirling of sterile water and the negative pressure of the square frame mouth drainage, but will be directly flushed out above the discharge trough 49, and then fall onto the second screen 53 in the aggregate frame under the obstruction of the baffle, thereby realizing the automatic screening of the Chinese orchid tissue culture seedlings, so that the abnormal tissue culture seedlings and the tissue culture seedlings with unqualified root development are separated onto the second screen 53, thereby effectively improving the overall survival rate and finished product rate of the Chinese orchid seedlings.

[0050] The wastewater discharged from the square frame mouth will pass through the through groove 413 on the discharge trough 49 and the screen 2 53 in the aggregate frame in turn, and finally enter the discharge pipe 46 through the return pipe 51 to be discharged. The multiple through holes on the receiving box 52 are used to allow the water droplets on the tissue culture seedlings entering it to fall into the aggregate frame and be discharged together with the wastewater. After that, the staff will regularly clean and remove the abnormal tissue culture seedlings and tissue culture seedlings with unqualified root development on the screen 2 53, and carry out corresponding treatment. At the same time, the qualified seedlings will be taken out and transplanted to the planting rack 6 after final disinfection and drying.

[0051] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A device for transplanting tissue culture seedlings of Chinese orchids, comprising a conveying mechanism (1), a mounting frame 1, a mounting frame 2, a mounting frame 3 and a planting frame (6), wherein a tissue culture bottle (2) is placed on the conveying mechanism (1), and a culture medium and tissue culture seedlings are placed in the tissue culture bottle (2), characterized in that: Also includes: A movable lifting mechanism and a removal mechanism (3), wherein the movable lifting mechanism is arranged at a top end of the mounting frame, and the removal mechanism (3) is arranged on the movable lifting mechanism and is located directly above the tissue culture bottle (2), and is used to remove the tissue culture seedlings in the tissue culture bottle (2) and prevent the roots and bases of the tissue culture seedlings from being damaged; The cleaning and screening mechanism comprises a cleaning component (4) and a screening component (5), the cleaning component (4) comprises a cleaning frame, an opening is provided at the top of the cleaning frame, and a feed port (41) is provided on the outer wall near the removal mechanism (3), a second liquid inlet pipe (45) is installed at the outer wall of the cleaning frame near the top, a stirring component is provided on the opening, a soft brush (411) is installed on the stirring component, and is used to make the soft brush (411) rotate along the opening while realizing self-rotation, the soft brush (411) is located in the cleaning frame, a square frame opening is provided on the outer wall of the cleaning frame, a gate valve (48) is installed on the square frame opening, and the height of the square frame opening corresponds to the height of the tissue culture bottle (2), and the width of the square frame opening is 1.2 times the conventional width of the tissue culture seedling, the screening component (5) is provided on the mounting frame three and the cleaning component (4), and is used to cooperate with the cleaning component (4) to effectively screen the cleaned tissue culture seedlings.

2. The device for transplanting cymbidium tissue culture seedlings according to claim 1, wherein: The bottom of the cleaning frame is respectively provided with a liquid inlet pipe (43) and a discharge pipe (46), a bubble generator (44) is installed at the connection between the liquid inlet pipe (43) and the cleaning frame, a ball valve (47) is installed on the discharge pipe (46), a screen (412) is installed near the bottom of the cleaning frame, the bottom of the soft brush (411) is in contact with the top of the screen (412), and a discharge trough (49) is installed at a downwardly inclined position near the bottom of the square frame opening.

3. The device for transplanting cymbidium tissue culture seedlings according to claim 2, wherein: The stirring component includes a mounting frame, which is fixedly mounted on the opening and has a top end connected to the mounting frame. A motor three (42) is mounted at the center of the top of the mounting frame, and an inner gear ring is mounted on the inner side wall. The output end of the motor three (42) passes through the top wall of the mounting frame and is fixedly connected to a cross bar. A rotating frame is mounted on one end of the cross bar and is rotatably connected to a gear (410) through the rotating frame. The gear (410) is meshed with the inner gear ring. The top end of the soft brush (411) passes through the bottom wall of the rotating frame and is fixedly connected to the bottom end of the gear (410).

4. The device for transplanting cymbidium tissue culture seedlings according to claim 2, wherein: The screening component (5) includes a material collection frame, which is installed at the top of the mounting frame three and has a screen two (53) installed inside. A material receiving box (52) is installed at the top of the screen two (53). The material receiving box (52) is located near the end of the material collection frame away from the cleaning frame, and a plurality of through holes are provided on its inner bottom wall. The height of the discharge trough (49) corresponds to the height of the culture medium in the tissue culture bottle (2), and a through groove (413) is provided on the inner top wall and the inner bottom wall of the discharge trough (49). The width of the through groove (413) corresponds to the conventional root diameter of the tissue culture seedling. The bottom wall of the outward end of the discharge trough (49) is connected to the top wall of the material receiving box (52) near the cleaning frame. A return pipe (51) is installed at the bottom end of the material collection frame and is connected to the discharge pipe (46) through the return pipe (51).

5. The device for transplanting cymbidium tissue culture seedlings according to claim 4, wherein: Two baffles are symmetrically mounted on the top of the aggregate frame. Both baffles are tilted outward and are located on both sides of the discharge chute (49).

6. The device for transplanting cymbidium tissue culture seedlings according to claim 1, wherein: The conveying mechanism (1) includes a conveyor belt, a bracket and a driving assembly. The bracket is arranged on the ground, the driving assembly is arranged at the top of the bracket, the conveyor belt is installed on the driving assembly, and a plurality of positioning grooves (11) are opened on the conveyor belt. The tissue culture bottle (2) is placed in the positioning groove (11). The movable lifting mechanism includes a moving part (32) and a mounting plate (33). The mounting plate (33) is installed on the moving part (32). The moving part (32) is arranged at the top of the mounting frame and is used to enable the mounting plate (33) to achieve displacement. Two cylinders (31) are installed at the top of the mounting plate (33). The output ends of the two cylinders (31) both penetrate the top wall of the mounting plate (33) and are fixedly connected to the removal mechanism (3).

7. The device for transplanting cymbidium tissue culture seedlings according to claim 6, wherein: The removal mechanism (3) includes a fixed plate, which is mounted on the output ends of the two cylinders (31) and has a motor 1 (34) mounted on the top. A rotating rod is mounted on the output end of the motor 1 (34). The bottom end of the rotating rod passes through the top wall of the fixed plate and is fixedly connected to the connecting plate. A plurality of connecting rods (36) are evenly mounted on the bottom end of the connecting plate. The bottom ends of the plurality of connecting rods (36) are fixedly connected to a mounting ring. The bottom end of the mounting ring is tilted inward, and a groove is provided on the tilted surface of the inner wall. Four rotating cavities (1) are evenly opened near the bottom of the mounting ring. A small gear (38) is rotatably connected in the rotating cavity (1). The positions of the four small gears (38) correspond to the positions of four connecting rods (36) in the plurality of connecting rods (36). A connecting rod is installed at the bottom of the small gear (38). The bottom end of the connecting rod passes through the bottom wall of the rotating cavity (1) and the top wall of the groove in sequence, and is fixedly connected to a scraper (37). The scraper (37) is tilted inward. The connection between the connecting rod and the scraper (37) is close to the edge of one end of the scraper (37). The four scrapers (37) are all located in the groove. The side wall of the scraper (37) close to the groove is in contact with the inner wall of the groove. A power assembly is provided on the connecting plate, the mounting ring and the plurality of connecting rods (36) for rotating the four small gears (38).

8. The device for transplanting cymbidium tissue culture seedlings according to claim 7, characterized in that: The outer diameter of the connecting plate is the same as the outer diameter of the mounting ring. The outer diameters of the connecting plate and the mounting ring are 5 mm smaller than the inner diameter of the tissue culture bottle (2). The angle between the inwardly inclined surface of the scraper (37) and the horizontal plane is 45 degrees.

9. The device for transplanting cymbidium tissue culture seedlings according to claim 7, wherein: The power assembly includes a cavity, a rotating cavity 2 and a motor 2 (35). The cavity and the rotating cavity 2 are both opened inside the mounting ring. The cavity is rotatably connected to an outer gear ring (311) and is communicated with the inside of the four rotating cavities 1. The outer gear ring (311) is meshed with four pinion gears 1 (38). The rotating cavity 2 is rotatably connected to a pinion gear 2 (39) and is communicated with the inside of the cavity. The outer gear ring (311) is meshed with the pinion gear 2 (39). The motor 2 (35) is fixedly mounted on the top of the connecting plate, and its output end is fixedly connected to a driving rod. The bottom end of the driving rod passes through the connecting plate, the corresponding connecting rod (36), the top wall of the mounting ring and the top wall of the rotating cavity 2 in sequence, and is fixedly connected to the top of the pinion gear 2 (39).

10. The device for transplanting cymbidium tissue culture seedlings according to claim 9, characterized in that: A plurality of piezoelectric ceramics (312) are fixedly embedded on the side walls of the four scrapers (37) away from the groove, and four wires (310) are installed in the mounting ring. The positions of the four wires (310) correspond to the positions of the four pinion gears (38). The bottom ends of the wires (310) sequentially penetrate the corresponding top wall of the rotating cavity, the pinion gear (38), the connecting rod and the top wall of the scraper (37), and are electrically connected to the plurality of piezoelectric ceramics (312) on the scraper (37). The top ends of the wires (310) sequentially penetrate the inner wall of the mounting ring, the corresponding connecting rod (36) and the connecting plate, and are connected to the external power supply.

Images