Cutting propagation method for rapid rooting of phytolacca acinosa

Abstract

The invention relates to the technical field of cutting propagation, and discloses a cutting propagation method for rapid rooting of pokeberry root, which comprises the following steps: S1, taking semi-lignified current-year branches as cutting slips, and soaking the cutting slips in a sterilizing and rooting complexing agent; s2, the treated cutting slips are inserted into a cutting medium; s3, a period of time after cuttage is conducted, fermentation liquor is poured, and the water content of the cuttage medium is controlled in the whole cuttage period. According to the phytolacca acinosa cutting propagation method provided by the invention, the sterilized rooting agent can promote root growth, inhibit bacteria, reduce and improve the stress resistance of cutting slips, and promote base cell activation; and the provided cutting medium ensures the required air permeability and drainage, so that the rooting and survival rate of the pokeberry root are improved.

Description

Technical Field

[0001] The invention relates to the technical field of cutting propagation, in particular to a cutting propagation method for rapid rooting of pokeweed. Background Art

[0002] In recent years, Traditional Chinese Medicine (TCM) has demonstrated unique efficacy in treating specific diseases, leading to its increasing global recognition as a complementary and alternative medicine. Pokeweed (Phytolacca acinosa Roxb.) is a traditional Chinese medicinal herb widely distributed throughout my country. Its medicinal value stems from the dried roots of Phytolacca acinosa Roxb. or Phytolacca americana L., both members of the Phytolacca family. The roots of Pokeweed possess anti-inflammatory, immune-enhancing, antibacterial, antiviral, and antitumor properties, and are included in the 2020 edition of the Chinese Pharmacopoeia for their significant medicinal value.

[0003] At this stage, research on pokeweed is mainly focused on applications in medicine, food, industry, and agriculture, with few reports on research related to pokeweed propagation techniques. Currently, the main methods of propagation for pokeweed are seed propagation and fleshy root colonization. Seed propagation has a low coefficient of propagation, but the seed germination rate is also low, and a long growing period is required. Generally, it takes at least three years from sowing to harvesting to achieve the required medicinal efficacy. The fleshy root colonization propagation technique is to select fleshy roots with buds and colonize them to propagate seedlings before the perennial roots of pokeweed sprout. The fleshy root colonization technique is relatively difficult, the breeding process is rigorous, and it also takes more than three years to achieve the required medicinal efficacy. Therefore, there is a need to find a suitable pokeweed propagation method to solve the problems of the long breeding period and rigorous breeding technology of pokeweed. Summary of the Invention

[0004] Technical problems solved by the present invention:

[0005] The invention provides a cutting propagation method for pokeweed, aiming to solve the technical problem that the current pokeweed propagation efficiency is low and is not conducive to the industrialization of pokeweed.

[0006] The technical solution adopted in the present invention is:

[0007] In view of the above technical problems, the object of the present invention is to provide a cutting propagation method for rapid rooting of Phytolacca americana.

[0008] Specifically,

[0009] A cutting propagation method for rapid rooting of Phytolacca japonica comprises the following steps:

[0010] S1 uses semi-lignified current year branches as cuttings and soaks the cuttings in a sterilizing rooting compound;

[0011] S2 inserts the treated cuttings into the cutting medium;

[0012] S3: After a period of time after the cutting, the fermentation liquid is poured, and the moisture content of the cutting medium is controlled at 40-50% during the entire cutting period.

[0013] According to some preferred embodiments, in S1, the cuttings are tender branches grown in the current year, with a diameter of 0.3-0.7 cm and a length of 10-15 cm. All leaves are removed from the branches, and 2-5 growth points are retained on the cuttings.

[0014] According to some preferred embodiments, in S1, the sterilization and rooting compound comprises a solution prepared by 100-200 mg / L indoleacetic acid (IAA), 100-200 mg / L indolebutyric acid (IBA), 100-200 mg / L naphthaleneacetic acid (NAA), 20-50 mg / L abscisic acid (ABA), 5-10 mg / L vitamin B2, 20-50 mg / mL vitamin C, 2-5 mg / L nicotinic acid, 5-10 mg / L 6-BA, and 500-1000-fold diluted carbendazim. When used, the cuttings are soaked in the solution for 10-30 minutes at room temperature.

[0015] According to some preferred embodiments, in S2, the water content of the cutting medium is adjusted to 60-70%, and cutting holes are drilled in the cutting medium, with a diameter of 0.7-1.0 cm and a depth of 4-6 cm.

[0016] According to some preferred embodiments, in S2, the raw material components of the cutting medium include, by weight, 20 to 45 parts of fine river sand, 10 to 30 parts of perlite, 20 to 40 parts of yellow-brown soil, 2 to 8 parts of biochar, 2 to 4 parts of humus, 2 to 5 parts of decomposed coconut coir, 2 to 5 parts of earthworm castings, and 1 to 2 parts of vermiculite.

[0017] According to some preferred embodiments, in S2, the pH value of the cutting medium ranges from 4.5 to 6.5, and the water content is 50 to 60%.

[0018] According to some preferred embodiments, in S2, the cutting medium is disinfected with 0.1-0.2% calcium cyanamide or 0.1-0.5% potassium permanganate, and after disinfection, it is placed in a sealed shed for 20 days to avoid disinfectant residue.

[0019] According to some preferred embodiments, the stems and leaves or roots of Phytolacca japonica are used as a fermentation system, and the fermentation liquid and fermentation residue obtained by fermentation are added to the cutting process of Phytolacca japonica, which can not only avoid the problem of competition with the microorganisms in the cutting matrix caused by the addition of exogenous bacteria, but also avoid the problem of limited survival period of exogenous bacteria in the cutting matrix. By adding its own residual fermentation body (fermentation liquid / fermentation residue), the secondary metabolites contained in it are utilized to match the growth needs of Phytolacca japonica. At the same time, since it is a symbiotic bacterial community with Phytolacca japonica obtained during the fermentation process of its own residual body, the risk of invasion of external bacteria can be avoided. Specifically, the stems and leaves or roots of Phytolacca japonica are ground and mixed with water (m 商 Land: water = 1:2 to 1:5), add 0.1 to 0.5% of the total weight of pokeweed and water Bacillus subtilis (10 9 CFU / g~10 10 CFU / g) is anaerobically fermented at room temperature for 7 to 15 days, and the fermentation liquid and fermentation residue are filtered. The fermentation liquid is diluted to 5 to 10% (by volume). After disinfection, the fermentation residue and fermentation liquid are added to the cutting medium. The diluted fermentation liquid is added at a ratio of 0.2 to 0.3% of the total weight of the cutting medium, and the cutting medium is balanced for 48 hours before use. The fermentation residue is added at a ratio of 15 to 30% of the total weight of the cutting medium.

[0020] According to some preferred embodiments, in S3, on days 8 to 15 after cutting, the cuttings are irrigated with a 10-50 mg / L gibberellin (GAs) and 0.3% potassium dihydrogen phosphate solution, with a weight ratio of water to cutting medium of 2:3-8. Subsequent care is performed according to conventional methods. This additional addition can increase the germination rate of the cuttings and promote rooting and germination.

[0021] According to some preferred embodiments, in S3, 1-3% fermentation broth is sprayed weekly between the 20th and 40th day after cutting. Subsequent maintenance is performed according to conventional methods. Spraying the fermentation broth during the initial rooting phase replenishes active substances. The fermentation broth contains IAA, cytokinins, and rooting enzymes synthesized by pokeweed itself, thus utilizing its own substances to promote root growth.

[0022] The beneficial effects achieved by the present invention are:

[0023] (1) The present invention provides a method for propagating Phytolacca japonica cuttings by treating the cuttings with a sterilizing and rooting agent. Through the synergistic effect of the various components of the sterilizing and rooting agent, the cuttings are used to promote root growth, inhibit bacteria, reduce and enhance the stress resistance of the cuttings, and promote the activation of base cells.

[0024] (2) The present invention provides a cutting propagation method for pokeweed, and a cutting medium that ensures the required air permeability and drainage, thereby improving the rooting and survival rate of pokeweed. At the same time, pokeweed residue and fermentation liquid are added to the cutting medium, utilizing the secondary metabolites obtained from the fermentation to promote the rooting and development of pokeweed.

[0025] (3) The present invention provides a method for propagating Phytolacca japonica by cuttings, which uses fermentation liquid for spraying after cuttings. The allelopathic substances contained in Phytolacca japonica itself can inhibit pathogens, thereby achieving the purpose of promoting root growth by Phytolacca japonica itself. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a physical picture of the cutting matrix in Example 1;

[0027] Figure 2 This is a morphological diagram of the lower cut and growing point of the Chinese pokeweed cuttings in Example 1;

[0028] Figure 3 This is the standard socket diagram of the cutting matrix in Example 1;

[0029] Figure 4 This is a physical picture of the new buds sprouting from the pokeweed cuttings in Example 1;

[0030] Figure 5 This is a physical picture of the morphology of new shoots 10 days after the cuttings of Phytolacca americana in Example 1;

[0031] Figure 6 This is a physical picture of the morphology of new shoots 30 days after the cuttings of Phytolacca americana in Example 1;

[0032] Figure 7 This is a physical picture of the rooting of the cuttings after the cuttings of Phytolacca americana in Example 1;

[0033] Figure 8 This is a physical picture of the rooting of the cuttings of Phytolacca japonica (without retaining the growing point) after the cuttings are cut in Example 1;

[0034] Figure 9 This is a physical picture of the rooting of the cuttings (the growing point is at the bottom) after the cuttings of Phytolacca japonica in Example 1;

[0035] Figure 10 This is a rooting rate trend chart when different numbers of growth points are retained on Phytolacca americana cuttings. DETAILED DESCRIPTION

[0036] To make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are described clearly and completely below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer are used. Where the manufacturer of the reagents or instruments is not specified, all are conventional products that can be purchased commercially.

[0037] Example 1

[0038] First, purchase the raw materials of the cutting medium, sieve the fine river sand, yellow-brown soil, and perlite, then dry them in the sun, and mix the biochar, humus, decomposed coconut coir, and dried fine river sand, yellow-brown soil and perlite in proportion by mass: 35% fine river sand, 15% perlite, 35% yellow-brown soil, 5% biochar, 3% humus, 3% decomposed coconut coir, 3% earthworm castings, and 1% vermiculite to make the cutting medium.

[0039] Then use a small amount of wood ash and ferrous sulfate to adjust the pH value of the cutting medium to 5.5, and finally disinfect the cutting medium with 0.5% potassium permanganate disinfectant. After disinfection, place the cutting medium in a sealed shed for 20 days to age.

[0040] The remaining pokeweed stems and leaves were crushed and mixed with water at a ratio of 1:3, and then 0.3% of the total weight of Bacillus subtilis (1.2*10 9 CFU / g) at room temperature for 15 days, and the fermentation broth and fermentation residue were filtered. After the fermentation broth was diluted to 5%, it was added at a ratio of 0.3% of the total weight of the cutting medium and allowed to equilibrate for 48 hours. The fermentation residue was added at a ratio of 15% of the total weight of the cutting medium.

[0041] Fill the above-mentioned cutting medium into a flower pot with a diameter of 30 cm and a height of 25 cm, and make cutting holes with a diameter of 1.0 cm and a depth of 5 cm on the medium for use.

[0042] Dilute 50% carbendazim solution 500 times for later use, weigh 100 mg of indoleacetic acid, 100 mg of indolebutyric acid, 100 mg / L of naphthaleneacetic acid, 30 mg / L of abscisic acid, 8 mg / L of vitamin B2, 30 mg / mL of vitamin C, 3 mg / L of nicotinic acid, and 8 mg / L of 6-BA, and add them to 1 L of the above-mentioned 500-fold diluted carbendazim solution as a sterilizing solution to obtain a sterilizing rooting compound for later use.

[0043] Use semi-lignified current-year branches as cuttings, trim the lower part of the cuttings and ensure that 2 to 3 growth points are left on the upper part of the cuttings. Then, soak the lower cut end of the cuttings in a sterilized rooting compound solution for 15 minutes.

[0044] Insert the lower part of the sterilized cuttings into the prepared cutting medium and irrigate the cutting medium with water. After that, water in time to ensure that the moisture content of the soil in the root matrix of the plant is within the range of 50-60%. Until the 10th day, irrigate with a concentration of 20 mg / L gibberellin and 0.3% potassium dihydrogen phosphate solution (that is, 1L of water contains 20 mg of gibberellin and 3 mg of potassium dihydrogen phosphate), and the weight ratio of water to cutting medium is 2:5. On the 20th day, spray with 3% fermentation liquid, and the ratio of water spraying to cutting medium is 1:0.2, once a week. Finally, weed and water according to conventional methods.

[0045] Example 2

[0046] This embodiment differs from embodiment 1 in that the amounts of the raw materials used in the cutting medium are different. Specifically, the cutting medium is prepared as follows: 35% fine river sand, 20% perlite, 30% yellow-brown soil, 4% biochar, 4% humus soil, 2% decomposed coconut husk, 3% earthworm castings, and 2% vermiculite.

[0047] Example 3

[0048] The difference between this embodiment and embodiment 1 is that the raw material components of the sterilization and rooting compound are different.

[0049] Specifically:

[0050] A 50% carbendazim solution was diluted 700 times for later use, and 200 mg of indoleacetic acid, 100 mg of indolebutyric acid, 120 mg / L of naphthaleneacetic acid, 20 mg / L of abscisic acid, 10 mg / L of vitamin B2, 20 mg / mL of vitamin C, 2 mg / L of nicotinic acid, and 5 mg / L of 6-BA were weighed and added to 1 L of the above-mentioned 700-fold diluted carbendazim solution as a sterilization solution to obtain a sterilization rooting compound.

[0051] Comparative Example 1

[0052] The difference between this comparative example and Example 1 is that no decomposed coconut husk and earthworm castings were added to the cutting medium.

[0053] Comparative Example 2

[0054] The difference between this comparative example and Example 1 is that no vermiculite was added to the cutting medium.

[0055] Comparative Example 3

[0056] The difference between this comparative example and Example 1 is that no fermentation liquid was added to the cutting medium.

[0057] Comparative Example 4

[0058] The difference between this comparative example and Example 1 is that no fermentation residue is added to the cutting medium.

[0059] Comparative Example 5

[0060] The difference between this comparative example and Example 1 is that fermentation liquid was not sprayed.

[0061] Comparative Example 6

[0062] The difference between this comparative example and Example 1 is that indolebutyric acid is not added to the sterilization and rooting compound.

[0063] Comparative Example 7

[0064] The difference between this comparative example and Example 1 is that vitamins and niacin are not added to the sterilization and rooting compound.

[0065] Comparative Example 8

[0066] The difference between this comparative example and Example 1 is that 6-BA is not added to the sterilization and rooting compound.

[0067] Test example

[0068] Figure 1 This is a physical picture of the cutting matrix in Example 1. Figure 1 It can be seen that the cutting medium has a loose structure and is gray-brown in color.

[0069] Figure 2 This is a morphological diagram of the lower cut and growth point of the Chinese pokeweed cuttings in Example 1. Figure 2 As can be seen, the lower cut of the cutting is a 45° bevel, forming a horseshoe shape. The growth points on the upper part of the cutting are distributed on both sides of the cutting, showing a single leaf alternate pattern. In addition, some of the growth points are the petioles where the leaves previously grew.

[0070] Figure 3 This is a diagram of the standard socket of the cutting medium in Example 1. The standard socket is poked out by a round wooden stick.

[0071] Figure 4 This is a real picture of the new buds of Phytolacca americana cuttings in Example 1. Figure 4 It can be seen that the position where the new buds grow is the previous growth point, and no new buds will germinate if the growing cuttings are not retained.

[0072] Figure 5 This is a physical picture of the morphology of new shoots 10 days after the cuttings of Phytolacca americana in Example 1. Figure 5 It can be seen that after 10 days of growth, the new shoots have grown new leaves, while the cuttings that were not retained have withered and turned yellow.

[0073] Figure 6 This is a physical picture of the morphology of new shoots 30 days after the cuttings of Phytolacca americana in Example 1. Figure 6 It can be seen that after 30 days of growth, the new buds have successfully grown new branches.

[0074] Figure 7 This is a photo of the rooting of the cuttings after the cuttings of Phytolacca americana in Example 1. Figure 7 It can be seen that the cuttings with growth points and new branches and leaves have successfully taken root, but the cuttings without growth points ( Figure 8 ) and cuttings with the growing point at the bottom ( Figure 9 ) will not take root but will wither directly.

[0075] Figure 10 This is a rooting rate trend diagram when different numbers of growth points are retained on the Chinese pokeweed cuttings in Example 1. Figure 10 It can be seen that as the number of retained growth points increases, the probability of cuttings germinating and taking root increases. When the number of retained growth points is 5, the probability of Phytolacca americana cuttings germinating and taking root reaches 83.7%, indicating that the retention of growth points is an important factor for the germination and rooting of Phytolacca americana cuttings.

[0076] In each example and comparative example, at least 20 cuttings of Phytolacca americana were used for cutting, and the rooting rate and survival rate of the cuttings were calculated 40 days after the cutting. The results are shown in Table 1.

[0077] Table 1 Statistical results

[0078] sample Survival rate (%) Rooting rate (%) Example 1 17 83.7 Example 2 15 75.0 Example 3 12 60.0 Comparative Example 1 16 80.4 Comparative Example 2 17 83.7 Comparative Example 3 14 70.6 Comparative Example 4 16 80.4 Comparative Example 5 16 80.4 Comparative Example 6 7 35.4 Comparative Example 7 9 45.7 Comparative Example 8 10 50.0

[0079] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A cutting propagation method for rapid rooting of Phytolacca japonica, characterized in that: The steps include: S1 uses semi-lignified current year branches as cuttings and soaks the cuttings in a sterilizing rooting compound; S2 inserts the treated cuttings into the cutting medium; S3: After a period of time after cutting, water the fermentation liquid and control the moisture content of the cutting medium during the entire cutting period.

2. The method for rapid rooting of Phytolacca americana according to claim 1, wherein In S1, the cuttings were tender branches grown in the current year, with a diameter of 0.3 to 0.7 cm and a length of 10 to 15 cm.

3. The method for rapid rooting of Phytolacca americana according to claim 1, wherein: In S1, the water content of the cutting medium is adjusted to 60-70%, and cutting holes are drilled in the cutting medium. The diameter of the cutting holes is 0.7-1.0 cm and the depth is 4-6 cm.

4. The method for rapid rooting of Phytolacca americana according to claim 1, wherein In S1, the sterilization and rooting compound includes a solution prepared by 100-200 mg / L indoleacetic acid, 100-200 mg / L indolebutyric acid, 100-200 mg / L naphthaleneacetic acid, 20-50 mg / L abscisic acid, 5-10 mg / L vitamin B2, 20-50 mg / mL vitamin C, 2-5 mg / L nicotinic acid, 5-10 mg / L 6-BA and carbendazim.

5. The cutting propagation method for rapid rooting of Phytolacca americana according to claim 1, wherein In S3, on the 20th to 40th day after cutting, 1 to 3% fermentation liquid was sprayed once a week, and the ratio of spraying water to cutting medium was 1:0.1 to 0.

3.

6. The method for rapid rooting of Phytolacca americana according to claim 1, wherein: In S3, on the 8th to 15th day after cutting, water with 10-50 mg / L gibberellin and 0.3% potassium dihydrogen phosphate solution, and the weight ratio of watering amount to cutting medium is 2:3-8.

7. The method for rapid rooting of Phytolacca americana by cuttings according to any one of claims 1 to 6, characterized in that: In S2, the raw materials of the cutting medium are calculated by weight and include 20-45 parts of fine river sand, 10-30 parts of perlite, 20-40 parts of yellow-brown soil, 2-8 parts of biochar, 2-4 parts of humus, 2-5 parts of decomposed coconut coir, 2-5 parts of earthworm castings, and 1-2 parts of vermiculite.

8. The method for rapid rooting of Phytolacca americana according to claim 7, wherein: In S2, the cutting medium is disinfected with 0.1-0.2% calcium cyanamide or 0.1-0.5% potassium permanganate.

9. The method for rapid rooting of Phytolacca americana according to claim 7, wherein: Fermentation liquid and / or fermentation residue are added to the cutting medium; the stems, leaves or roots of Phytolacca japonica are ground and crushed, and then mixed with water; Bacillus subtilis is added to perform anaerobic fermentation at room temperature; and the fermentation liquid and fermentation residue are obtained by filtering; the fermentation liquid is diluted and then added to the cutting medium.

10. The method for rapid rooting of Phytolacca americana by cutting propagation according to claim 9, characterized in that: m 商陆 :m 水 =1:2~1:5; and / or, the amount of Bacillus subtilis added accounts for 0.1~0.5% of the total weight of pokeweed and water; and / or, the fermentation time is 7~15 days.

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