Method for improving growth of dendrobium officinale seedlings

Abstract

The invention relates to a method for improving growth of dendrobium officinale seedlings. The dendrobium officinale seedlings are illuminated through combined light waves. The method has the advantages that growth is promoted, the increase of the content of polysaccharide, mannose and dendrobine of dendrobium officinale seedlings is remarkably influenced, and the content of endogenous auxin is increased.

Description

Technical Field

[0001] The invention relates to a method for improving the growth of Dendrobium officinale seedlings. Background Art

[0002] An existing floating hardening and cultivation method for Dendrobium officinale tissue culture seedlings includes the following steps: 1) establishing a floating hardening pool: site selection, pool construction, and scaffolding; 2) selecting and preparing the substrate; 3) managing the tissue culture seedlings before and after transplanting; 4) using floating seedling trays and transplanting methods; and 5) using a nutrient solution for hardening the tissue culture seedlings. However, this method has the disadvantage of not effectively promoting the growth of the Dendrobium officinale seedlings or increasing their polysaccharide content. Summary of the Invention

[0003] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a method for improving the growth of Dendrobium officinale seedlings.

[0004] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solution: a method for improving the growth of Dendrobium officinale seedlings, which promotes the growth of Dendrobium officinale tissue culture seedlings by combined light wave irradiation.

[0005] Furthermore, the combined light waves are red light, blue light, and green light.

[0006] Furthermore, the wavelength of red light is 650 nanometers, the wavelength of blue light is 470 nanometers, and the wavelength of green light is 530 nanometers.

[0007] Furthermore, the ratio of red light: blue light: green light is 2:2:1.

[0008] Furthermore, the light intensity is 1500 lux.

[0009] Furthermore, the Dendrobium officinale seedlings were cultivated in pots and exposed to a combination of light waves for 14 hours and darkness for 10 hours every day. The culture room temperature was 28 degrees Celsius in light and 22 degrees Celsius in darkness. After 2 months, the growth status of the Dendrobium officinale seedlings and the polysaccharide content therein were tested.

[0010] The beneficial effects of the present invention are: promoting the growth of Dendrobium officinale seedlings, increasing the content of polysaccharides, mannose and dendrobium alkaloids, and increasing the content of endogenous auxin through combined light wave irradiation. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 Figure 2 shows the growth of Dendrobium officinale seedlings in Example 1 and the control group. DETAILED DESCRIPTION

[0012] The following is a combination of the embodiments of the present invention Figure 1 , the technical solutions in the embodiments of the present invention are described clearly and completely. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0013] Example 1 reference Figure 1 , a method for improving the growth of Dendrobium officinale seedlings, promoting the growth of Dendrobium officinale tissue culture seedlings by combined light wave irradiation.

[0014] A method for improving the growth of Dendrobium officinale seedlings, comprising combining light waves into red light, blue light, and green light.

[0015] A method for improving the growth of Dendrobium officinale seedlings, comprising red light with a wavelength of 650 nanometers, blue light with a wavelength of 470 nanometers, and green light with a wavelength of 530 nanometers.

[0016] A method for improving the growth of Dendrobium officinale seedlings, wherein the ratio of red light: blue light: green light is 2:2:1.

[0017] A method for improving the growth of Dendrobium officinale seedlings, comprising a light intensity of 1500 lux.

[0018] A method for improving the growth of Dendrobium officinale seedlings, comprising: a light treatment group: cultivating the Dendrobium officinale seedlings in pots, irradiating them with a combination of light waves for 14 hours and darkness for 10 hours per day, with the culture chamber temperature being 28°C during light and 22°C during darkness; and detecting the growth status of the Dendrobium officinale seedlings and their polysaccharide content after two months.

[0019] In the control group, Dendrobium officinale seedlings were cultivated in pots under normal light conditions for 14 hours and darkness for 10 hours daily. The incubator temperature was 28°C during light and 22°C during darkness. After two months, the growth status and polysaccharide content of the seedlings were measured. Comparative data are shown in Tables 1 and 2.

[0020] Table 1 is a comparison of the growth status of Dendrobium officinale seedlings in the light treatment group and the control group

[0021] Table 2 shows the polysaccharide content of Dendrobium officinale seedlings in the light treatment group and the control group

[0022] The effects of the control group with normal light and the light treatment group with combined light wave irradiation on the growth of Dendrobium officinale were compared. The results showed that the application of red light 650nm: blue light 470nm: green light 530nm; 2:2:1 combination light waves can promote the growth of Dendrobium officinale seedlings, significantly increase the content of polysaccharides, mannose, and dendrobine, and increase the content of endogenous auxin.

[0023] The above-described embodiments merely represent several implementations of the present invention. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous modifications and improvements without departing from the scope of the present invention. Therefore, the scope of protection of the patent for this invention shall be subject to the appended claims.

Claims

1. A method for improving the growth of Dendrobium officinale seedlings, characterized in that :Promoting the growth of Dendrobium officinale tissue culture seedlings through combined light wave irradiation.

2. A method for improving the growth of Dendrobium officinale seedlings according to claim 1, characterized in that :The combined light waves are red light, blue light and green light.

3. A method for improving the growth of Dendrobium officinale seedlings according to claim 1, characterized in that : Red light wavelength 650 nanometers: Blue light wavelength 470 nanometers: Green light wavelength 530 nanometers.

4. The method for improving the growth of Dendrobium officinale seedlings according to claim 2, wherein the ratio of red light: blue light: green light is 2:2:

1.

5. The method for improving the growth of Dendrobium officinale seedlings according to claim 3, wherein: The light intensity is 1500 lux.

6. The method for improving the growth of Dendrobium officinale seedlings according to claim 1, wherein: The Dendrobium officinale seedlings were cultivated in pots and exposed to a combination of light waves for 14 hours and darkness for 10 hours every day. The culture room temperature was 28 degrees Celsius in the light and 22 degrees Celsius in the dark. The growth status of the Dendrobium officinale seedlings and their polysaccharide content were tested after 2 months.

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