Tomato leaf miner egg collecting device and using method thereof

Abstract

The invention relates to the field of tomato leaf miner control, in particular to a tomato leaf miner egg collecting device and a using method thereof.The tomato leaf miner egg collecting device comprises a first container and a second container, a gauze element is detachably embedded in the top of the first container, and a nutrition donor is arranged on the gauze element; a connecting piece is connected between the bottom of the first container and the top of the second container, a fixing piece is installed on the connecting piece, and the top of the second container is open. The inner cavity of the container II is used for filling a nutrient solution. The egg laying device which has a smooth inner wall and contains tomato plants is provided for the device, and the whole device is divided into a container I for collecting eggs at the upper part, a connecting piece and a container II for filling a nutrient solution at the lower part. The smooth inner wall of the device can prevent tomato leaf miners from laying eggs, tomato plants and leaves in the device can provide egg laying media for the tomato leaf miners, adults of the tomato leaf miners can lay eggs on the limited leaves in a concentrated mode, the tomato leaf miner eggs can be collected in a concentrated mode in the later period, and the egg obtaining rate is greatly increased. And the development of indoor related research is stably supported.

Description

Technical Field

[0001] The present invention relates to the field of tomato leafminer prevention and control, in particular to a tomato leafminer egg collection device and a use method thereof. Background Art

[0002] The tomato leafminer, Tuta absoluta Meyrick, native to Peru in South America, is a devastating pest of Solanaceae crops worldwide. Since invading Spain and other Mediterranean countries in 2006, it has rapidly spread to Asia, Europe, and Africa, drawing international attention for its rapid spread and severity. In 2017, the insect first invaded the Yili region of Xinjiang, my country, through agricultural product trade and was subsequently found in Lincang, Yunnan, in 2018. Subsequently, it rapidly spread to Guizhou, Yunnan, Hunan, Chongqing, Guangxi, Shaanxi, Shanxi, Inner Mongolia, Gansu, Qinghai, Ningxia, Liaoning, Hebei, Tianjin, Beijing, and Hebei.

[0003] The tomato leafminer has the following biological characteristics: (1) A wide range of host plants. According to statistics, the tomato leafminer can harm nearly 40 plant species, including fruits, vegetables, flowers, tobacco, and grains. Among them, it has a clear preference for Solanaceae crops and has extremely strong destructive power; (2) High reproductive level. Female insects can mate multiple times in their lifetime, and a single female can lay about 260 eggs, with some even laying up to 350 eggs; (3) Fast spread. Literature reports that the tomato leafminer migrates and spreads at a speed of 800 km per year; (4) Strong adaptability. The tomato leafminer can feed and harm tomato plants at any growth stage and any part of the plant. After hatching, the larvae will burrow into the leaf tissue to feed on the mesophyll, making them extremely hidden and difficult to detect. At the same time, the larvae also have the characteristics of rotating leaves and plants, which increases the degree of damage to crops. The insect can overwinter in severe cold conditions and can also harm in warm areas. It can survive and reproduce at an altitude of 1000-3500 meters; (5) Heavy overlapping generations. The tomato leafminer produces 10-12 generations annually, with the number of generations showing a strong positive correlation with temperature. Typically, eggs, larvae, pupae, and adults emerge simultaneously. Therefore, the tomato leafminer poses a serious threat and significant challenges to agricultural production safety in my country, particularly in the tomato, potato, and ginseng fruit industries.

[0004] Indoor artificial breeding is an important means and source of obtaining test insects. Effective indoor propagation and significantly increasing the yield of test insects are paramount for conducting indoor research on the tomato leafminer. For example, conducting routine biological, behavioral, ecological, and molecular biological research on the tomato leafminer requires access to various insect stages, including eggs. However, tomato leafminer eggs are laid in a scattered pattern, often on the undersides of leaves and in hidden areas of the plant. This biological quirkiness hinders the consistent acquisition of large numbers of eggs. Currently, the conventional method for collecting tomato leafminer eggs involves using entire tomato plants as ovipositors in large insect cages. This method has drawbacks such as cumbersome operation, difficulty in finding eggs, low egg recovery rates, easy escape of adults, the coexistence of multiple stages, and low operability. Therefore, a suitable device and egg collection method are crucial for collecting large quantities of tomato leafminer eggs indoors. Summary of the Invention

[0005] In response to the above-mentioned problems, the present invention proposes a tomato leafminer egg collection device and a method of use thereof. By providing a smooth inner wall that is not preferred for egg laying and tomato plants that are preferred for egg laying as egg-laying media, the adult insects are guided to lay eggs in a concentrated manner, thereby improving the egg collection efficiency. The invention aims to significantly improve the collection efficiency of tomato leafminer eggs, enhance operational feasibility, simplify the operating steps, and reduce the cost of propagation.

[0006] To achieve the above object, the present invention is implemented according to the following technical solutions:

[0007] A tomato leafminer egg collection device comprises a first container and a second container. The top of the first container is opened and a detachable gauze is embedded in the opening. A nutrient donor is arranged on the gauze. A connecting piece is connected between the bottom of the first container and the top of the second container. A fixing piece is installed on the connecting piece for fixing a tomato plant and keeping it upright. The upper part of the tomato plant is placed in the inner cavity of the first container, and the lower part of the tomato plant is placed in the inner cavity of the second container. The top of the second container is open. The inner cavity of the second container is used for filling with nutrient solution.

[0008] In the above technical solution, the inner wall of the container 1 is a smooth surface.

[0009] In the above technical solution, a zipper is installed on the gauze net.

[0010] In the above technical solution, the nutrient donor is a cotton ball soaked in honey water.

[0011] In the above technical solution, the connecting piece is ring-shaped, and the connecting piece is connected to the first container or the second container in a snap-fit connection.

[0012] In the above technical solution, the fixing member is a sponge layer, and a through hole for fixing the tomato plant is provided on the sponge layer.

[0013] A method for using a tomato leafminer egg collecting device is implemented according to any of the above-mentioned tomato leafminer egg collecting devices, comprising the following steps:

[0014] S1: preparing a nutrient solution suitable for the growth and development of tomato plants and filling it into the inner cavity of container 2;

[0015] S2: Connect the connector to the top of the second container, install a fixing piece on the connector, and fix the tomato plant on the fixing piece in an upright position; place the lower part of the tomato plant into the nutrient solution in the inner cavity of the second container;

[0016] S3: Connect the bottom of container 1 to the connecting piece;

[0017] S4: After the assembly is completed, the adult tomato leafminer can be guided into the inner cavity of the container through the gauze. After the gauze is closed, a nutrient donor is placed on the gauze to serve as a nutrient source for the adult to survive and lay eggs.

[0018] S5: placing the device in a tomato leafminer breeding environment and collecting tomato leafminer eggs at regular intervals in the early morning of the next day;

[0019] S6: During collection, the connector is removed from the top of container 2, and nutrient solution is replenished in time so that the tomato petioles can absorb sufficient nutrients for growth. The removed container 1 is inverted, and the main body of container 1 is gently shaken to separate the adult tomato leafminers from the tomato plants by utilizing their tendency to gather upward. At this point, the tomato plants with eggs can be removed from the lower part of the container by separating container 1 from the connector.

[0020] S7: Tomato plants with tomato leafminer eggs are placed in an open tray, and the eggs are swept into the tray with a fine brush. Finally, all the swept eggs are collected and sieved to remove impurities such as tomato leaves and adult tarsus to obtain tomato leafminer eggs.

[0021] In the above technical solution, in step S7, the fine brush is made of mixed hair.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] Based on the biological tendency of adult tomato leafminers to lay eggs on tomato plants and leaves, this invention provides an oviposition device with a smooth inner wall and containing tomato plants. The device consists of an upper container (Container 1) for collecting eggs, a connector between the intermediate containers 1 and 2, and a lower container (Container 2) for filling with nutrient solution. The smooth inner wall of the device prevents the tomato leafminers from laying eggs, while the tomato plants and leaves inside provide a medium for the moths to lay eggs, facilitating concentrated egg laying on a limited number of leaves. This further facilitates the later collection of tomato leafminer eggs, significantly improving egg recovery rates and steadily supporting the development of related indoor research.

[0024] This device is modularly assembled, and each component can be disassembled into independent components. It can be assembled, transported, and reused at will. It is suitable for different regions and different climatic conditions, which significantly improves the convenience of device assembly and the low cost of transportation. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0026] Figure 1 This is a schematic structural diagram of a tomato leafminer egg collecting device according to the present invention;

[0027] Figure numerals: 1 - container one; 2 - container two; 3 - gauze; 4 - nutrient donor; 5 - connecting piece; 6 - fixing piece. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments.

[0029] like Figure 1As shown, the present invention proposes a tomato leafminer egg collection device, including a container 1 and a container 2 2. For example, the container 1 and the container 2 2 can be wide-mouth bottles. The top of the container 1 is opened and a detachable gauze 3 is embedded in the opening, and a nutrient supplier 4 is provided on the gauze 3; a connecting piece 5 is connected between the bottom of the container 1 and the top of the container 2 2, and a fixing piece 6 is installed on the connecting piece 5 for fixing the tomato plant and keeping it upright. The upper part of the tomato plant is placed in the inner cavity of the container 1 1, and the lower part of the tomato plant is placed in the inner cavity of the container 2 2; the top of the container 2 2 is set to be open; the inner cavity of the container 2 2 is used for filling the nutrient solution.

[0030] The device of the present invention is composed of an upper container 1 for collecting eggs, a connector 5 connecting the intermediate container 1 and the second container 2, and a lower container 2 for filling with nutrient solution. This device is modularly assembled, and each component can be disassembled into independent parts, allowing for easy assembly, transportation, and reuse. It is suitable for different regions and climates, significantly improving the ease of assembly and the cost of transportation.

[0031] Specifically, the upper container 1 can have a volume of 1000 mL, primarily providing space for adult insects to move around and also serving as space for plant growth. The bottle is typically made of smooth glass or plastic. Firstly, the material is relatively inexpensive, effectively controlling costs. Secondly, the smooth inner wall discourages adult tomato leafminers from attaching, allowing them to lay their eggs on tomato leaves, making egg collection more convenient. The top of container 1 is a removable, breathable mesh 3 with a 1 mm pore size, secured with a removable zipper. The mesh 3 primarily provides sufficient air for the adult tomato leafminers within container 1, ensuring their survival. The removable zipper is primarily used for loading and unloading adult tomato leafminers and also provides access for replacing tomato plants. The nutrient donor 4 provides the necessary nutrients for the adult tomato leafminer to ensure its normal survival and egg-laying. The nutrient donor 4 is a cotton ball soaked in honey water. The tomato plant (containing about 3-4 true leaves) in the device mainly serves as a medium for the tomato leafminer to lay eggs, attracting the adult to lay eggs on the tomato leaves. The purpose is to quickly find and separate the eggs attached to the leaves, improve the collection efficiency and reduce the damage rate to the eggs. The tomato leaves with petioles are collected from the tomato plants on the same day. The leaves are fresh and have good vitality. The petioles are stuck in the fixing part 6. Firstly, the petioles are immersed in the container 2 2 to absorb the nutrients in the nutrient solution for the normal growth of the tomato leaves. Secondly, the petioles are stuck in the fixing part 6, which mainly plays a fixing and supporting role.

[0032] Connector 5 is typically a plastic, annular, double-mouthed, snap-on bottle cap. This snap-on design facilitates disassembly and assembly of container 1 (1) and container 2 (2). Fastener 6 is a 1 cm thick, high-density sponge embedded in the center of the cap. This sponge layer serves three primary functions: first, it secures the tomato leaves in an upright position, ensuring sufficient egg-laying medium for adult tomato leafminers; second, the breathable sponge layer allows evaporated moisture from container 2 (2) to pass smoothly through it into container 1 (1), providing the necessary humidity for the survival of adult tomato leafminers and eggs; and third, it forms a physical barrier, preventing adult tomato leafminers from entering container 2 (2).

[0033] Container 2 (2) can have a volume of 1000 mL. Its primary function is to provide the necessary nutrients and water for the normal survival of tomato leaves, ensuring their vitality and freshness during the egg-laying process of the tomato leafminer. This improves the attraction of adult tomato leafminers and increases the egg recovery rate. Furthermore, the water in Container 2 (2) evaporates through the sponge layer, providing the necessary humidity for the adult tomato leafminers and eggs in Container 1 (1).

[0034] A method for using a tomato leafminer egg collecting device of the present invention is implemented based on the above-mentioned tomato leafminer egg collecting device, and includes the following operating steps:

[0035] S1: Prepare a nutrient solution suitable for the growth and development of tomato plants. The nutrient solution formula is shown in Table 1 to improve the growth of tomatoes and attract adult insects to lay eggs, and place it into the inner cavity of container 2;

[0036] Table 1 Nutrient solution formula

[0037]

[0038] S2: Connect the connector 5 to the top of the second container 2 and tighten the buckle. Install a high-density sponge layer with a diameter of 2 cm on the connector 5, and make a through hole with a length of 1 cm in the middle of the sponge layer. Secure the tomato plant in an upright position in the through hole of the sponge layer; place the lower part of the tomato plant in the nutrient solution in the inner cavity of the second container 2;

[0039] S3: Connect the bottom of container 1 to the connector 5 and fasten the buckle; forming a tomato leafminer egg collection device consisting of container 1, connector 5, and container 2;

[0040] S4: After assembly is completed, adult tomato leafminers can be led into the inner cavity of container 1 through the zipper on the gauze 3. For example, 5 female tomato leafminers that have emerged for 24 hours and 10 male adults can be led into container 1. After closing the zipper, a cotton ball soaked in honey water can be placed on the gauze 3 as a source of nutrition for the adults to survive and lay eggs.

[0041] S5: placing the device in a tomato leafminer breeding environment, such as a temperature of 26±1°C, a relative humidity of 60±5%, and a photoperiod of 16L:8D, and collecting tomato leafminer eggs at regular intervals in the early morning of the next day;

[0042] S6: During collection, the connector 5 is disassembled from the top of the container 2, and the nutrient solution is replenished in time so that the tomato petioles can absorb sufficient nutrients for growth; the disassembled container 1 is inverted, and the main body of the container 1 is gently shaken to separate the adult tomato leafminers from the tomato plants by taking advantage of their tendency to gather upward; at this point, the container 1 is separated from the connector, and the plant with eggs can be taken out from under the container 1;

[0043] S7: Tomato plants with tomato leafminer eggs are placed in an open tray, and the eggs are swept into the tray with a fine brush. Finally, all the swept eggs are collected and sieved to remove impurities such as tomato leaves and adult tarsus to obtain tomato leafminer eggs.

[0044] The present invention is based on the biological learning tendency of adult tomato leafminers that they prefer to lay eggs on tomato plants and leaves. By providing an egg-laying device with a smooth inner wall and containing tomato plants, the smooth inner wall of the device can prevent the tomato leafminers from laying eggs, while the tomato plants and leaves inside can provide an egg-laying medium for the tomato leafminers, which is conducive to the concentrated egg-laying of adult tomato leafminers on limited leaves, and is more conducive to the concentrated collection of tomato leafminer eggs in the later stage, which will greatly improve the egg acquisition rate and stably support the development of related indoor research.

[0045] Test Example 1: Selectivity of adult tomato leafminers for different oviposition media

[0046] (1) Test materials

[0047] The egg-laying selective device consisted of a 15 cm × 15 cm × 15 cm insect cage with two smooth and two rough plastic surfaces. Two smooth and two rough plastic pieces were fixed to each of the four sides of the cage, forming an oviposition area composed of the cage mesh, rough and smooth plastic surfaces. The artificial climate chamber (RXZ-500, Ningbo Jiangnan Instrument Factory) maintained a temperature of 26 ± 1°C, a relative humidity of 60 ± 5%, and a photoperiod of 16 L:8 D.

[0048] (2) Test method

[0049] Five female and ten male adults of the tomato leafminer (Cyprinus spp.), 24 hours after emergence, were introduced into the aforementioned oviposition selection device. The device was maintained at a temperature of 26 ± 1°C, a relative humidity of 60 ± 5%, and a photoperiod of 16 L:8 D. A 10% honey solution was provided as a nutritional source for the adults. Ten replicates were used. After 48 hours, the number of eggs on the sarong, smooth, and rough plastic surfaces was observed and recorded, and the oviposition selection rate was calculated.

[0050] (3) Test results

[0051] Results showed that adult tomato leafminers laid eggs on all surfaces, including gauze, smooth plastic, and rough plastic. However, the egg production on rough plastic was significantly higher than on both gauze and smooth plastic, while the egg production on gauze was slightly higher than on smooth plastic. Furthermore, adult tomato leafminers had a high egg-laying rate of 86.44% on rough plastic, compared to only 8.23% on gauze and 5.33% on smooth plastic. This suggests that adult tomato leafminers prefer rough plastic for egg-laying. To increase the number of eggs laid by adult tomato leafminers on tomato plants and improve egg collection efficiency and yield, smooth plastic was selected as the primary component of the oviposition device.

[0052] Table 2 Oviposition effects of adult tomato leafminers on gauze, smooth plastic and rough plastic

[0053]

[0054]

[0055] Test Example 2: Oviposition selectivity of adult tomato leafminers at various locations within the device

[0056] (1) Test materials

[0057] The experimental apparatus consisted of a lower container 2, an intermediate connector 5, and an upper container 1. Based on the results of Experimental Example 1, the upper container 1 was constructed of a plastic material with a smooth inner surface. The artificial climate chamber (RXZ-500, Ningbo Jiangnan Instrument Factory) was maintained at a temperature of 26±1°C, a relative humidity of 60±5%, and a photoperiod of 16 L:8 D. Other experimental materials included tomato plants, a high-density sponge, nutrient solution, breathable gauze, and absorbent cotton.

[0058] (2) Test method

[0059] After assembling the device according to the method of the present invention, three female adults, 24 hours after emergence, and six male adults were introduced into Container 1. They were then placed in an incubator for mating and oviposition. Ten replicates were performed. After 48 hours, the number of eggs laid by adult tomato leafminers on smooth plastic bottles, high-density sponges, gauze, and tomato plants was counted, and the egg-laying selection rate was calculated. The results are shown in Table 3.

[0060] (3) Test results

[0061] Results showed that the average egg production of adult tomato leafminers on smooth plastic bottles, high-density sponges, gauze, and tomato plants was 13.3, 10.9, 16.3, and 134.2 eggs, respectively. The egg selection rates for these four locations were 7.59%, 5.98%, 9.64%, and 76.79%, respectively. The egg production on tomato plants was significantly higher than that on smooth plastic bottles, high-density sponges, and gauze. This indicates that the tomato plants within the egg collection device are highly attractive to adult tomato leafminers for egg laying, significantly increasing the egg recovery rate. Therefore, the device can be used as an efficient egg-laying device for collecting tomato leafminer eggs.

[0062] Table 3 Oviposition selectivity of adult tomato leafminers at various locations within the device

[0063] Treatment group Smooth plastic material / granules High density sponge / granules Gauze / grains Tomato plants / grains 1 9 0 12 79 2 0 2 21 118 3 13 9 14 161 4 15 11 16 120 5 12 19 24 136 6 21 12 12 147 7 25 9 19 158 8 8 15 9 173 9 17 18 13 98 10 13 14 23 152 average value 13.3 10.9 16.3 134.2 Average selection rate 7.59％ 5.98％ 9.64％ 76.79％

[0064] Test Example 3: Destruction rate of tomato leafminer eggs by brushes of different hardness

[0065] (1) Test materials

[0066] The materials used in the experiment are brushes of different materials, including goat hair, wolf hair, and mixed hair.

[0067] (2) Test method

[0068] After assembling the device according to the method of use of the present invention, three female adults, 24 hours after emergence, and six male adults were introduced into the upper container (1). They were then placed in an incubator for mating and egg laying. Ten repetitions were performed. After 48 hours, the eggs of the tomato leafminer were gently brushed with a goat-hair, wolf-hair, or mixed-hair brush. The degree of damage to the eggs by the brushes of different materials during the egg collection process was measured.

[0069] (3) Test results

[0070] As shown in Table 4, the results show that the rate of egg damage caused by brushes during the egg collection process for tomato leafminers varies depending on the brush material. The egg damage rates for goat hair, wolf hair, and mixed hair brushes were 10.80%, 11.88%, and 5.79%, respectively. From the perspective of the hardness of the brush material, goat hair brushes, being too soft, lack the necessary force to collect the eggs in one stroke, preventing them from being swept down in one go. Repeated sweeping and brushing can damage the eggs. Wolf hair brushes, being harder, can easily puncture the eggs during the sweeping process. Mixed hair brushes, on the other hand, combine the softness of goat hair brushes with the hardness of wolf hair brushes, allowing for a single sweep of the eggs, significantly improving collection efficiency and reducing egg damage. Therefore, mixed hair brushes are suitable for egg collection.

[0071] Table 4 Destruction rate of tomato leafminer eggs by brushes of different hardness

[0072]

[0073] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present invention should be included in the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the scope of protection of the claims.

Claims

1. A device for collecting tomato leafminer eggs, characterized in that: The invention comprises a container (1) and a container (2), wherein the top of the container (1) is opened and a detachable gauze (3) is embedded in the opening, and a nutrient supply (4) is arranged on the gauze (3); a connecting piece (5) is connected between the bottom of the container (1) and the top of the container (2); a fixing piece (6) is installed on the connecting piece (5) for fixing a tomato plant and keeping it upright, the upper part of the tomato plant is placed in the inner cavity of the container (1), and the lower part of the tomato plant is placed in the inner cavity of the container (2); the top of the container (2) is open; the inner cavity of the container (2) is used for filling nutrient solution.

2. The tomato leafminer egg collecting device according to claim 1, characterized in that: The inner wall of the container (1) is smooth.

3. The tomato leafminer egg collecting device according to claim 2, characterized in that: A zipper is installed on the gauze net (3).

4. The tomato leafminer egg collecting device according to claim 3, characterized in that: The nutrient donor (4) is a cotton ball soaked in honey water.

5. The tomato leafminer egg collecting device according to claim 4, characterized in that: The connecting piece (5) is annular, and the connecting piece (5) is connected to the container one (1) or the container two (2) in a snap-fit connection.

6. The tomato leafminer egg collecting device according to claim 5, characterized in that: The fixing member (6) is a sponge layer, and a through hole for fixing the tomato plant is provided on the sponge layer.

7. A method for using a tomato leafminer egg collection device, characterized in that: The method is implemented according to the tomato leafminer egg collecting device according to any one of claims 1 to 6, comprising the following steps: S1: prepare a nutrient solution suitable for the growth and development of tomato plants and fill it into the inner cavity of container two (2); S2: Connect the connecting piece (5) to the top of the second container (2), install the fixing piece (6) on the connecting piece (5), and fix the tomato plant on the fixing piece (6) in an upright state; the lower part of the tomato plant is placed in the nutrient solution in the inner cavity of the second container (2); S3: Connect the bottom of container 1 (1) to the connecting piece (5); S4: After the assembly is completed, the adult tomato leafminer can be guided to the inner cavity of the container (1) through the gauze (3). After the gauze (3) is closed, a nutrient donor (4) is placed on the gauze (3) as a nutrient source for the adult to survive and lay eggs; S5: placing the device in a tomato leafminer breeding environment and collecting tomato leafminer eggs at regular intervals in the early morning of the next day; S6: When collecting, the connecting piece (5) is disassembled from the top of the container two (2), and the nutrient solution is replenished in time so that the tomato petioles can absorb sufficient nutrients for growth; the disassembled container one (1) is turned upside down, and the main body of the container one (1) is gently shaken to separate the tomato leafminer adults from the tomato plants by utilizing the habit of the tomato leafminer adults to gather upwards; at this time, the tomato plants with eggs can be taken out from the lower part of the container one (1) by separating the container one (1) from the connecting piece (5); S7: Tomato plants with tomato leafminer eggs are placed in an open tray, and the eggs are swept into the tray with a fine brush. Finally, all the swept eggs are collected and sieved to remove impurities such as tomato leaves and adult tarsus to obtain tomato leafminer eggs.

8. The method for using the tomato leafminer egg collection device according to claim 7, characterized in that: In step S7, the fine brush is made of mixed hair.

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