Composition for promoting plant growth and preventing and treating plant diseases and application thereof

Abstract

The invention provides a composition, and the composition comprises physcion and amino-oligosaccharin. The composition comprises physcion and amino-oligosaccharin. The physcion and amino-oligosaccharin composition is jointly applied to plants, and the two active ingredients play a synergistic effect, so that the composition not only has a growth promoting effect, but also has a prevention and treatment increasing effect, so that the symptoms of the tobacco plants are relieved, the disease index is reduced, and the accumulation amount of viruses in the plants is reduced.

Description

Technical Field

[0001] The present disclosure belongs to the field of plants, and particularly relates to a composition for promoting plant growth and preventing and controlling plant diseases and its application. Background Art

[0002] Plant viral diseases are the second most common plant disease after fungal diseases. They are characterized by their diverse species, wide host range, severe damage, and difficulty in prevention and control. Viral infections often cause severe declines in crop yield and quality, posing a significant threat to crop production safety. Adjustments in cropping patterns and climate change have led to an increasing prevalence of plant viral diseases in my country, characterized by sudden, explosive, and recurring outbreaks, severely impacting grain production and the security of the supply chain for critical agricultural products.

[0003] Currently, the prevention and control of plant viral diseases in production largely relies on agricultural measures and disease-resistant varieties. However, the prevention and control of many plant viral diseases still faces prominent challenges, such as a lack of resistant varieties and the deterioration of resistance. Antiviral agents remain the ideal, most direct and effective means of preventing and controlling plant viral diseases. Currently, there is an extreme shortage of plant viral disease prevention and control agents. While there are some antiviral products on the market, such as guanidine hydrochloride, amino oligosaccharides, chlorfenapyr, and cinclostrobin, plant viruses are complex and infect a wide variety of crops. Registered antiviral agents account for less than 0.5% of all registered pesticides. Therefore, the development of more effective, broad-spectrum, and environmentally friendly agents to prevent plant viral diseases remains crucial for agricultural development. Summary of the Invention

[0004] In order to overcome the shortcomings and deficiencies in the prior art, the present disclosure provides a highly effective and broad-spectrum composition for resisting plant viral diseases and promoting plant growth, which uses physcion and amino oligosaccharides as active ingredients, and provides the use of the above composition in resisting plant viral diseases (such as tobacco potato virus Y, wheat yellow mosaic virus, etc.). When the physcion and amino oligosaccharides composition is jointly applied to plants, the two active ingredients exert a synergistic effect, not only having a growth-promoting effect, but also having an enhanced control effect, thereby alleviating tobacco plant symptoms, reducing disease indexes, and reducing the amount of virus accumulation in the plant. Moreover, the above plant antiviral disease composition is a biological agent, is environmentally friendly, safe and environmentally friendly, and has important application prospects in resisting plant viral diseases, preventing and controlling plant diseases caused by plant viruses, and improving plant quality.

[0005] According to one aspect of the present disclosure, a composition is provided, comprising physcion and amino oligosaccharides.

[0006] In some embodiments, the weight ratio of the physcion methyl ether to the amino oligosaccharide is 1:(1-20), preferably 1:(1-10), and more preferably 1:(1-5). The inventors have found that when the mass ratio of physcion methyl ether to amino oligosaccharide is controlled within the above range, the synergistic effect is further improved. In some specific embodiments, the weight ratio of the physcion methyl ether to the amino oligosaccharide is 1:1, 1:1.25, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.125, 1:4, 1:5, 1:6.25, 1:6.5, 1:7, 1:8, 1:10, 1:12, 1:15, 1:18, 1:20 or any value therebetween.

[0007] In some embodiments, the composition further comprises a solvent.

[0008] In some embodiments, the solvent comprises an organic solvent.

[0009] In some embodiments, the solvent includes one or more of dimethyl sulfoxide, ethyl acetate, acetone, isopropanol, 2,2,2-trifluoroethanol, propylene carbonate, benzene, toluene, xylene, dimethylformamide, dimethyl sulfoxide, dichloromethane, cyclohexane, cyclohexanone, N-methylpyrrolidone or solvent oil.

[0010] In some embodiments, the concentration of physcion in the composition is 0.02-0.2 mg / L, preferably 0.05-0.1 mg / L. In some embodiments, the concentration of physcion in the composition is 0.02 mg / L, 0.04 mg / L, 0.05 mg / L, 0.08 mg / L, 0.1 mg / L, 0.12 mg / L, 0.15 mg / L, 0.18 mg / L, 0.2 mg / L, or any value therebetween.

[0011] In some embodiments, the concentration of the amino oligosaccharide in the composition is 0.1-0.5 mg / L, preferably 0.125-0.5 mg / L. In some specific embodiments, the concentration of the amino oligosaccharide in the composition is 0.1 mg / L, 0.125 mg / L, 0.2 mg / L, 0.25 mg / L, 0.3 mg / L, 0.35 mg / L, 0.4 mg / L, 0.45 mg / L, 0.5 mg / L or any value therebetween.

[0012] In some embodiments, the dosage form of the composition includes emulsion, solution, suspension, granules or powder.

[0013] According to another aspect of the present disclosure, provided is a use of the composition of the present disclosure in promoting plant growth.

[0014] In some embodiments, the promoting plant growth includes promoting plant height and / or promoting plant root growth.

[0015] In some embodiments, promoting plant root growth includes increasing the main root length, number of lateral roots, fresh weight and / or dry weight of the plant.

[0016] In some embodiments, the plant comprises an herb.

[0017] In some embodiments, the plant comprises tobacco, wheat, corn, or sorghum.

[0018] According to another aspect of the present disclosure, provided is a use of the composition of the present disclosure in preventing and controlling plant diseases.

[0019] In some embodiments, the controlling of plant diseases includes one or more of inhibiting plant virus replication, controlling plant diseases caused by plant viruses, or resisting plant viral diseases.

[0020] In some embodiments, the plant diseases include one or more of mosaic virus disease, stripe virus disease, necrosis virus disease, and deformity virus disease of plants.

[0021] In some embodiments, the plant virus comprises potato virus Y or wheat yellow mosaic virus.

[0022] In some embodiments, the plant comprises an herb.

[0023] In some embodiments, the plant comprises tobacco, wheat, corn, or sorghum.

[0024] According to yet another aspect of the present disclosure, there is provided a method for promoting plant growth and / or controlling plant diseases, comprising applying the composition of the present disclosure to the plant.

[0025] The active components of the composition disclosed herein include physcion and amino oligosaccharides. Amino oligosaccharides can activate immune factors in plants, induce plants to develop disease resistance, and have preventive, inhibitory and killing effects on a variety of fungi, bacteria, and viruses. They can induce cotton to respond to Verticillium wilt and changes in the activity of related defense enzymes. Physcion is a natural anthraquinone compound extracted from the traditional Chinese medicine plant rhubarb. It not only has a bactericidal effect, but also can activate the plant's active immune system. It has the characteristics of low toxicity, fast degradation, and low residue. The present invention utilizes amino oligosaccharides to enhance the immunity of tobacco plants, and uses physcion as an auxiliary for bactericidal treatment. The two work together to alleviate the spread of tobacco virus diseases and provide technical support for the prevention and control of tobacco virus diseases.

[0026] Unexpectedly, in the present disclosure, when physcion and amino oligosaccharide compositions are jointly applied to plants, the two active ingredients exert a synergistic and synergistic effect, which not only has a growth-promoting effect, but also has an increased prevention and control effect, so that the symptoms of tobacco plants are alleviated, the disease index decreases, and the amount of virus accumulation in the plants is reduced. Moreover, the above-mentioned plant antiviral disease composition is a biological agent, which is environmentally friendly, safe and environmentally friendly. It has important application prospects in resisting plant viral diseases, preventing and controlling plant diseases caused by plant viruses, and improving plant quality. DETAILED DESCRIPTION

[0027] To make the objectives, technical solutions, and advantages of the present disclosure more clearly understood, the present disclosure is further described in detail below with reference to the following embodiments. The specific embodiments described herein are intended only to illustrate the present disclosure and are not intended to limit the present disclosure in any way. In addition, descriptions of well-known structures and technologies are omitted in the following description to avoid unnecessary confusion about the concepts of the present disclosure. Such structures and technologies are also described in many publications.

[0028] The reagents and / or kits used in the following examples are all commercially available or can be synthesized by known methods. Example 1 Effect of combined application of physcion and amino oligosaccharides on tobacco plant growth

[0029] 1. Preparation of experimental materials

[0030] The test agent, 90% amino oligosaccharide technical, was purchased from Zhongxiang Yipinhong Plant Immunity Technology Co., Ltd., and 98% physcion technical was purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd. Both agents were dissolved in dimethyl sulfoxide to prepare a stock solution concentration of 1000 mg / L. The stock solution was diluted to the required experimental concentrations, with final concentrations of physcion methyl ether of 0.05, 0.08, and 0.1 mg / L, and amino oligosaccharide of 0.125 and 0.5 mg / L.

[0031] The test plant was Nicotiana benthamiana. Seedlings of 4-8 leaves were selected that were uniform in size, growth, and free of disease and insects.

[0032] 2. Chemical treatment

[0033] The experiment was conducted in an artificial climate chamber at a temperature of 25°C, a relative humidity of 50%-60%, and a light intensity of 6000-7000 Lx. The agent was diluted with water and evenly sprayed on the upper surface of leaves of uniformly growing tobacco plants. A control (CK) sprayed with plain water was used. Spraying was performed once every 7 days, for a total of 2 sprayings. Six treatments were used (Table 1), with six plants sprayed in each treatment, i.e., six replicates.

[0034] Table 1 Experimental treatments and dosages of pesticides

[0035] Chemical treatment Physcion methyl ether (mg / L) Aminooligosaccharides (mg / L) Physcion 0.1 - amino oligosaccharides - 0.5 Physcion methyl ether + amino oligosaccharides 0.05 0.5 Physcion methyl ether + amino oligosaccharides 0.08 0.5 Physcion methyl ether + amino oligosaccharides 0.1 0.5 comparison - -

[0036] 3. Growth Index Measurement

[0037] Plant height was measured before the first spraying, 7 days after the first spraying, and 7 days after the second spraying. Root length, number of lateral roots, and whole-plant fresh and dry weight were also measured 7 days after the second spraying. The results are shown in Tables 2 and 3.

[0038] Table 2 Effects of physcion methyl ether combined with amino oligosaccharides on tobacco plant height growth (unit: cm)

[0039]

[0040] Note: Data are mean ± standard error. Different lowercase letters indicate significant differences between treatments at the 0.05 level as determined by DMRT test.

[0041] As shown in Table 2, the effect of physcion combined with amino oligosaccharides on promoting tobacco plant height was significantly better than that of a single spray. After two sprays of different single sprays, the seedling height was significantly higher than that of the control. Seven days after the first spray, the plant height of the physcion-treated group increased by 15.41% compared to the control, and the amino oligosaccharide-treated group increased by 15.63%. Seven days after the second spray, the plant height increases were 21.34% and 27.81%, respectively. The amino oligosaccharide-treated group had a significantly higher plant height than the physcion-treated group, demonstrating a good growth-promoting effect. The effects of physcion combined with amino oligosaccharides at different concentrations on tobacco seedling height were significantly higher than those of a single spray, with tobacco plant height increasing by 1.79% to 12.14% compared to amino oligosaccharide alone.

[0042] Table 3 Effects of physcion combined with chitosan oligosaccharide and brown algae oligosaccharide on the main root length, lateral root number, fresh weight and dry weight of tobacco

[0043]

[0044] Note: Data are mean ± standard error. Different lowercase letters indicate significant differences between treatments at the 0.05 level as determined by DMRT test.

[0045] As shown in Table 3, both physalis and amino oligosaccharides exhibited a significant effect in promoting root growth. The taproot length and fresh weight of the physalis combined treatment were significantly higher than those of either treatment alone. The taproot length, number of lateral roots, fresh weight, and dry weight of the combined physalis and amino oligosaccharide treatment were significantly higher than those of the amino oligosaccharide treatment alone, with increases ranging from 25.24% to 30.74%, 2.15% to 16.20%, 9.58% to 14.72%, and 7.56% to 42.44%, respectively. This indicates that physalis combined with amino oligosaccharides has a significant effect on promoting tobacco growth.

[0046] Example 2 Inhibition rate of virus by treatment with physcion combined with amino oligosaccharides

[0047] 1. Preparation of experimental materials

[0048] The test agent, 90% amino oligosaccharide technical, was purchased from Zhongxiang Yipinhong Plant Immunity Technology Co., Ltd., and 98% physcion technical was purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd. Both agents were dissolved in dimethyl sulfoxide to prepare a stock solution concentration of 1000 mg / L. The stock solution was diluted to the required experimental concentrations, with physcion concentrations of 0.05, 0.08, and 0.1 mg / L and amino oligosaccharide concentrations of 0.125 and 0.5 mg / L.

[0049] The test plant was Nicotiana benthamiana. Seedlings of 4-8 leaves were selected that were uniform in size, growth, and free of disease and insects.

[0050] Potato virus Y (PVY) carrying the green fluorescent protein marker gene was provided by the plant pathology team of the Institute of Plant Protection and Soil and Fertilizer, Hubei Academy of Agricultural Sciences.

[0051] Other reagents and instruments mainly include: quartz powder (passed through a 600-mesh sieve), carbonate buffer solution (CBS, pH 9.6), potato virus Y enzyme-linked immunosorbent assay kit (Shanghai Yuanju Biotechnology Co., Ltd.), enzyme-linked immunosorbent assay instrument (Berteng Instrument Co., Ltd., USA), 365 nm long-wave ultraviolet lamp (SPECTROLINE, USA), grinder, etc.

[0052] 2. Chemical treatment

[0053] The experiment was conducted in an artificial climate chamber at a temperature of 25°C, a relative humidity of 50%-60%, and a light intensity of 6000-7000 Lx. The agent was diluted with water and evenly sprayed on the upper surface of leaves of uniformly growing tobacco plants. A control (CK) sprayed with plain water was used. Spraying was performed once every 7 days for a total of 2 sprayings. Thirteen treatments were included (Table 4), with 6 plants sprayed per treatment, i.e., 6 replicates.

[0054] Table 4

[0055]

[0056] 3. Virus Preparation and Inoculation Methods

[0057] The virus inoculation test was carried out 2 hours after the second spraying. First, a small amount of quartz powder was evenly sprinkled on the front of the three expanded leaves below the unexpanded young leaves of tobacco. Each leaf was inoculated with 50 μL of virus juice containing PVY by friction inoculation. Six plants were inoculated for each treatment, i.e., six replicates. After inoculation, the plants were placed in an artificial climate chamber for further cultivation. After 5 days, the young leaves of each plant inoculated with the virus were taken, wrapped with tin foil and numbered, quickly frozen with liquid nitrogen, and stored in a -80°C refrigerator for use.

[0058] 4. ELISA detection of viral content and calculation of viral inhibition rate

[0059] 5 days after virus inoculation, the leaf samples of different treatments were ground according to the ratio of 0.1 g (leaf weight): 1 mL (carbonate buffer solution), the grinding solution was poured into a centrifuge tube, centrifuged at 5000 r / min for 2 minutes, and the supernatant was taken into a clean centrifuge tube. The potato virus Y content in the sample was determined according to the operating procedures of the potato virus Y enzyme-linked immunosorbent assay kit, and expressed as the OD value at a wavelength of 450 nm. The potato virus Y content (OD 450 ) were used to calculate the virus inhibition rate of each treatment.

[0060] Virus inhibition rate = (control group OD 450 - Treatment group OD 450 ) / control group OD 450 ×100%.

[0061] The results are shown in Table 5.

[0062] Table 5 Inhibition rate of virus by physcion methyl ether combined with amino oligosaccharide

[0063]

[0064] Note: Data are mean ± standard error. Different lowercase letters indicate significant differences between treatments at the 0.05 level as determined by DMRT test.

[0065] As shown in Table 5, the concentration of virus in tobacco leaves in all treatment groups was significantly lower than in the control. The lowest concentration was observed in the combination of physcion 0.1 mg / L and oligosaccharide, followed by the combination of physcion 0.08 mg / L and oligosaccharide. Both treatments exhibited some inhibitory effect against polyvinyliform yellows (PVY), with physcion treatment being superior to oligosaccharide. Compared to either treatment alone, the combination of physcion and oligosaccharide exhibited greater PVY inhibition, with the physcion 0.1 mg / L and oligosaccharide 0.5 mg / L treatment achieving the highest viral inhibition rate of 84.78%, demonstrating a synergistic effect. In the control group, the physcion 0.1 mg / L and oligosaccharide 5 mg / L treatment achieved a viral inhibition rate of 77.17%, demonstrating a significantly lower synergistic effect than the physcion 0.1 mg / L and oligosaccharide 0.5 mg / L treatment. Example 3 Effect of Physcion Methyl Ether Combined with Oligosaccharides on Tobacco Potato Virus Y in Indoor Potted Plants

[0066] 1. Plant Materials

[0067] Refer to Example 2.

[0068] 2. Chemical treatment

[0069] Refer to Example 2.

[0070] 3. Virus Preparation and Inoculation Methods

[0071] Refer to Example 2.

[0072] 4. Plant symptom recording and prevention efficacy calculation

[0073] Observe plant symptoms 10 days after virus inoculation and record the severity of disease on the top 5 or 6 leaves of each plant. Calculate the disease index and calculate the efficacy. The specific method is based on the industry standard "Guidelines for Field Efficacy of Pesticides Part 73: Fungicides for the Control of Tobacco Virus Diseases" (NY / T 1464.73-2018). The grade standards are as follows:

[0074] Level 0: no mosaic lesions;

[0075] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0076] Level 3: The lesion area accounts for 5.1-15% of the entire leaf area;

[0077] Level 5: The lesion area accounts for 15.1-25% of the entire leaf area;

[0078] Level 7: The lesion area accounts for 25.1% to 50% of the entire leaf area;

[0079] Level 9: The lesion area accounts for more than 50.1% of the entire leaf area.

[0080] Calculation formula for disease index and disease index prevention effect:

[0081]

[0082] The results are shown in Table 6 below.

[0083] Table 6 Effect of combined application of physcion and amino oligosaccharides on PVY virus in potted plants

[0084] Chemical treatment Disease Index Disease control efficacy (%) Physcion methyl ether 0.05 mg / L 57.33 (36.15±0.19)g Physcion methyl ether 0.1mg / L 45.59 (49.39±0.47)e Aminoligosaccharides 0.125 mg / L 65.52 (27.34±0.33)h Aminoligosaccharides 0.5mg / L 58.14 (35.97±0.17)g Physcion methyl ether 0.05mg / L + amino oligosaccharides 0.125mg / L 47.47 (46.82±0.28)f Physcion methyl ether 0.05mg / L + amino oligosaccharides 0.25mg / L 44.94 (49.65±0.68)e Physcion methyl ether 0.0mg / L + amino oligosaccharides 0.5mg / L 42.01 (52.94±0.53)d Physcion methyl ether 0.08mg / L + amino oligosaccharides 0.125mg / L 40.15 (55.02±0.71)c Physcion methyl ether 0.08mg / L + amino oligosaccharides 0.25mg / L 38.63 (56.72±0.92)c Physcion methyl ether 0.08mg / L + amino oligosaccharides 0.5mg / L 37.04 (58.50±0,76)c Physcion methyl ether 0.1mg / L + amino oligosaccharides 0.125mg / L 25.87 (71.01±1.02)b Physcion methyl ether 0.1mg / L + amino oligosaccharides 0.25mg / L 23.46 (73.72±0.83)b Physcion methyl ether 0.1mg / L + amino oligosaccharides 0.5mg / L 19.81 (77.81±0.47)a Control: Physcion methyl ether 0.1 mg / L + Vanillyl thiocyanate 5 mg / L 35.63 (60.08±0.56)c Blank control 89.26

[0085] Note: Data are mean ± standard error. Different lowercase letters indicate significant differences between treatments at the 0.05 level as determined by DMRT test.

[0086] Table 6 shows the disease index and disease control efficacy of tobacco plants in different treatment groups. Compared to the control, the disease index decreased after spraying, indicating that the treatments inhibited the progression of tobacco potato virus Y (TPVY). The control tobacco leaf disease index was 89.26. Disease control efficacy was 36.15% and 49.39% in the 0.05 mg / L and 0.1 mg / L physcion methyl ether treatments, respectively. While efficacy was 27.34% and 35.97% in the 0.125 mg / L and 0.5 mg / L amino oligosaccharides treatments, respectively. In the treatments with different concentrations of physcion methyl ether and amino oligosaccharides, viral disease symptoms were significantly alleviated, and efficacy against PVY was significantly enhanced. The combination of physcion methyl ether (0.1 mg / L) and amino oligosaccharides aqueous solution achieved efficacy exceeding 70%, demonstrating a synergistic effect. Among them, the treatment with physcion (0.1 mg / L) combined with amino oligosaccharide aqueous solution (0.5 mg / L) had the highest control efficiency of 77.81%, with the most obvious synergistic effect. The control effect of the control group of physcion (0.1 mg / L) + vanillyl thiocarbamate (5 mg / L) was 60.08%, and the synergistic effect was significantly lower than that of physcion and amino oligosaccharide.

[0087] The technical solution of the present disclosure is not limited to the above-mentioned specific embodiments. Any technical variations made according to the technical solution of the present disclosure fall within the protection scope of the present disclosure.

Claims

1. A composition comprising physcion and amino oligosaccharides.

2. The composition according to claim 1, characterized in that The weight ratio of the physcion to the amino oligosaccharide is 1:(1-20), preferably 1:(1-10), and more preferably 1:(1-5).

3. The composition according to claim 1 or 2, characterized in that The composition further comprises a solvent, Preferably, the solvent comprises an organic solvent; More preferably, the solvent includes one or more of dimethyl sulfoxide, ethyl acetate, acetone, isopropanol, 2,2,2-trifluoroethanol, propylene carbonate, benzene, toluene, xylene, dimethylformamide, dimethyl sulfoxide, dichloromethane, cyclohexane, cyclohexanone, N-methylpyrrolidone or solvent oil.

4. The composition according to any one of claims 1 to 3, characterized in that In the composition, the concentration of physcion is 0.02-0.2 mg / L, preferably 0.05-0.1 mg / L; and / or The concentration of the amino oligosaccharide is 0.1-0.5 mg / L, preferably 0.125-0.5 mg / L.

5. The composition according to any one of claims 1 to 4, wherein The dosage form of the composition includes emulsion, solution, suspension, granule or powder.

6. Use of the composition according to any one of claims 1 to 5 in promoting plant growth.

7. The use according to claim 6, characterized in that The promoting of plant growth includes promoting plant height and / or promoting plant root growth; Preferably, the promoting plant root growth comprises increasing the main root length, the number of lateral roots, the fresh weight and / or the dry weight of the plant; Preferably, the plant comprises a herbaceous plant; More preferably, the plant comprises tobacco, wheat, corn, or sorghum.

8. Use of the composition according to any one of claims 1 to 5 in preventing and controlling plant diseases.

9. The use according to claim 6, characterized in that The plant disease prevention and control includes one or more of inhibiting plant virus replication, preventing and controlling plant diseases caused by plant viruses, or resisting plant viral diseases; Preferably, the plant diseases include one or more of mosaic virus disease, stripe virus disease, necrosis virus disease, and deformity virus disease of plants; More preferably, the plant virus comprises potato virus Y or wheat yellow mosaic virus; Preferably, the plant comprises a herbaceous plant; More preferably, the plant comprises tobacco, wheat, corn, or sorghum.

10. A method for promoting plant growth and / or preventing and controlling plant diseases, comprising applying the composition according to any one of claims 1 to 5 to the plant.

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