Anti-aging, tightening and soothing composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to an anti-aging, firming and soothing composition and a preparation method and application thereof, and the anti-aging, firming and soothing composition comprises the following components in parts by weight: 0.002-5 parts of ceramide, 0.1-50 parts of a bifidus yeast fermentation product filtrate and 0.1-10 parts of glassine. Ceramide can effectively repair a skin barrier, reduce water loss and enhance skin compactness; the bifidus yeast fermentation product filtrate is rich in various amino acids, vitamins and mineral substances, has excellent oxidation resistance and DNA damage repair capability, and can delay the skin aging process; the vitriol stimulates the generation of glycosaminoglycan and collagen by activating the synthesis of extracellular matrix protein of the skin, so that the elasticity of the skin is remarkably improved, and wrinkles are smoothed. The three components have a synergistic effect, the effects are exerted from multiple dimensions of oxidation resistance, cell repair, barrier reinforcement, collagen regeneration and the like, the problems of skin looseness, wrinkles, dryness, sensitivity and the like are effectively improved, and the efficient anti-aging, tightening and soothing effects are achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an anti-aging, firming and soothing composition, a preparation method and application thereof. Background Art

[0002] In modern society, people face high work pressures and often need to work late into the night. To cope with the stress of this intense workload, they rely on high-sugar foods and drinks like milk tea, cakes, and milk lattes to refresh themselves and relieve the pressure of work. Staying up late prevents the body from effectively eliminating free radicals. The increase in free radicals damages the structure of collagen and elastin, leading to sagging skin. High sugar intake leads to glycation of the skin, causing it to harden and lose elasticity. It also causes the body to maintain a low-grade inflammation over a long period of time, inhibiting the skin's ability to repair itself and causing increased pigmentation and wrinkles.

[0003] Current anti-aging cosmetics on the market offer limited efficacy, solely scavenging free radicals to achieve their anti-aging goals. These products are also quite irritating, requiring consumers to carefully consider their choices. However, due to social pressures, environmental pollution, and dietary changes, the skin of most people in modern society is more sensitive. Summary of the Invention

[0004] The purpose of the present invention is to overcome the shortcomings of the existing technology. The present invention provides an anti-aging, firming and soothing composition and its preparation method and application. The main ingredients are mild, suitable for a wide range of people, can repair the skin barrier, and have anti-aging and firming effects.

[0005] In order to solve the above problems, the present invention proposes an anti-aging, firming and soothing composition, comprising the following components in parts by weight:

[0006] Ceramide 0.002-5 parts

[0007] 0.1-50 parts of bifid yeast fermentation product

[0008] 0.1-10 parts of Bose

[0009] The ceramide includes at least one of ceramide AP, ceramide NP, ceramide EOP, ceramide AS, ceramide NS, and ceramide NG.

[0010] As an improvement of the above technical solution, the weight ratio of the ceramide, the bifid yeast fermentation product and bosine is 1:(1-3):(3-5).

[0011] As an improvement of the above technical solution, the bifid yeast fermentation product is a bifid yeast fermentation product nanomaterial formed by bifid yeast fermentation broth loaded with PEG-PCL, and the average particle size of the bifid yeast fermentation product nanomaterial is 130-160 nm.

[0012] As an improvement of the above technical solution, the average molecular weight of PEG used to synthesize the PEG-PCL is 5 kDa, and the average molecular weight of PCL used to synthesize the PEG-PCL is 2 kDa.

[0013] As an improvement to the above technical solution, the method for preparing the nanomaterial of the bifid yeast fermentation product comprises the following steps:

[0014] (1) PEG-PCL nanomicelles, bifid yeast fermentation broth, and methanol are mixed to form a mixed liquid, and a dry film is formed after rotary evaporation; wherein the volume ratio of the PEG-PCL nanomicelles to the bifid yeast fermentation broth is (5-10):1;

[0015] (2) After freeze-drying, PBS buffer solution is added to carry out water bath reaction, and after the reaction is completed, ultrasonic treatment, microporous membrane filtration, and freeze-drying are performed to obtain the nanomaterial of the bifid yeast fermentation product.

[0016] As an improvement of the above technical solution, the water temperature of the rotary evaporation in step (1) is 35-45°C;

[0017] In the step (2), the pH of the PBS buffer solution is 7.4, the water bath temperature of the water bath reaction is 35-45° C., the water bath reaction time is 10-20 min, and the pore size of the microporous filter membrane is 0.2 μm.

[0018] As an improvement of the above technical solution, the following components in parts by weight are also included:

[0019] Solubilizer 0.1-1 part

[0020] 5-15 parts moisturizer

[0021] 1.2-5.5 parts of preservatives

[0022] The solubilizing agent is SOLUBILISANT LRI;

[0023] The moisturizing agent includes one or more of glycerin, allantoin, trehalose, and betaine;

[0024] The preservative includes one or more of p-hydroxyacetophenone, 1,3-butanediol, 1,2-pentanediol, and 1,2-hexanediol.

[0025] Accordingly, the present invention also provides a method for preparing the anti-aging, firming and soothing composition as described above, comprising the following steps:

[0026] Add ceramide, bifid yeast fermentation product and bosine into a reactor according to weight parts, mix and stir evenly to obtain the product.

[0027] Correspondingly, the present invention also provides a use of the anti-aging, firming and soothing composition as described above in cosmetics.

[0028] As an improvement of the above technical solution, the cosmetic is toner, lotion, essence, cream, ointment, facial mask, gel or freeze-dried powder.

[0029] The implementation of the present invention has the following beneficial effects:

[0030] (1) The composition of the present invention contains ceramide, bifid yeast fermentation product filtrate and phosphatidylserine; ceramide is one of the components of the skin's stratum corneum cells, which can fill the gaps between the stratum corneum cells and play a positive role in repairing the skin barrier; phosphatidylserine can fill the gaps between the skin's extracellular matrix, making the skin firm; bifid yeast fermentation product filtrate is rich in various amino acids, vitamins and minerals, has excellent antioxidant and DNA damage repair capabilities, and can delay the aging process of the skin; among them, phosphatidylserine can improve skin elasticity and reduce wrinkles, and ceramide and bifid yeast fermentation product filtrate can repair the skin barrier. DETAILED DESCRIPTION

[0031] In order to make the objectives, technical solutions and advantages of the present invention more clear, the present invention will be described in further detail below.

[0032] The present invention provides an anti-aging, firming and soothing composition, comprising the following components in parts by weight:

[0033] Ceramide 0.002-5 parts

[0034] 0.1-50 parts of filtrate of bifid yeast fermentation product

[0035] 0.1-10 parts of Bose

[0036] The ceramide includes at least one of ceramide AP, ceramide NP, ceramide EOP, ceramide AS, ceramide NS, and ceramide NG.

[0037] The stratum corneum is the skin's protective barrier, composed of ceramides, fatty acids, cholesterol, and other substances. Today's stressful lives, coupled with a high-sugar and high-salt diet, contribute to glycation and aging of the stratum corneum.

[0038] Ceramides are a class of amide compounds formed by the dehydration of long-chain fatty acids and the amino group of sphingosine. Ceramides are a major component of the intercellular matrix, acting like the "mortar" between stratum corneum cells. They can fill cracks created by glycation in the skin, playing a crucial role in maintaining moisture balance in the stratum corneum and contributing to skin barrier repair.

[0039] Ceramide AP belongs to a specific subtype in the ceramide family. It is formed by sphingosine (a long-chain amino alcohol) and fatty acids connected by amide bonds. It is an important component of cell membranes and the stratum corneum of the skin.

[0040] Ceramide NP can form a complementary barrier repair mechanism with ceramide AP, enhance the skin's water-locking effect, inhibit the expression of pro-inflammatory factors such as IL-6, reduce inflammatory reactions such as rosacea and seborrheic dermatitis, and relieve burning and desquamation.

[0041] Ceramide EOP is formed by linking sphingosine and ω-hydroxy fatty acids through an amide bond. Its ω-hydroxy (ω-OH) structure can bind to the involucrin of the cornified envelope, strengthening the connection between the lipid matrix and corneocytes, forming a tighter physical barrier. It can reduce transepidermal water loss (TEWL) by forming a dense membranous lipid layer.

[0042] The filtrate from the fermentation product of bifid yeast is rich in lipid compounds with a rich and diverse lipid composition and high unsaturation. It can promote cell regeneration and accelerate melanin metabolism, thereby brightening the skin tone. It also resists oxidative stress and delays skin aging.

[0043] Bosin (hydroxypropyl tetrahydropyrantriol) is a glycoprotein mixture derived from xylose. It promotes the production of glucosaminoglycans, also known as mucopolysaccharides. Bosin promotes collagen synthesis, restoring skin elasticity, reducing dark spots and fine lines, and firming the skin for a youthful appearance.

[0044] Preferably, the weight ratio of ceramide, bifid yeast fermentation product, and bosin is 1:(1-3):(3-5). Exemplarily, the weight ratio of ceramide, bifid yeast fermentation product, and bosin is 1:1:3, 1:1:3, or 1:3:5, but is not limited thereto.

[0045] Bifid yeast fermentation products require large-scale cultivation of Bifidobacterium longum in an anaerobic environment, placing stringent demands on equipment sealing and temperature and humidity control. Fermentation cycles are long and energy-intensive, leading to long production cycles and high production costs. Human skin cells have limited absorption of bifid yeast fermentation products, necessitating the addition of large quantities of the product to the product to ensure adequate absorption.

[0046] Preferably, the bifid yeast fermentation product is a bifid yeast fermentation product nanomaterial formed by a bifid yeast fermentation broth loaded with PEG-PCL, and the average particle size of the bifid yeast fermentation product nanomaterial is 130-160 nm. The PCL hydrophobic segment carries hydrophobic drugs by physical encapsulation or chemical bonding, which can significantly improve the water dispersibility of fat-soluble drugs. In addition, the PEG hydrophilic shell forms a steric hindrance effect, reduces the aggregation between nanomicelles, and maintains the long-term stability of the micelle structure in body fluids. This avoids uneven distribution of the bifid yeast fermentation product on the skin. Wherein, the average particle size of the bifid yeast fermentation product nanomaterial is 130-160 nm. Exemplarily, 130 mm, 150 mm, 160 mm, but not limited thereto.

[0047] More preferably, the average molecular weight of PEG used to synthesize the PEG-PCL is 5 kDa, and the average molecular weight of PCL used to synthesize the PEG-PCL is 2 kDa.

[0048] Specifically, the method for preparing the nanomaterial of the bifid yeast fermentation product comprises the following steps:

[0049] (1) PEG-PCL nanomicelles, bifid yeast fermentation broth, and methanol are mixed to form a mixed liquid, and a dry film is formed after rotary evaporation; wherein the volume ratio of the PEG-PCL nanomicelles to the bifid yeast fermentation broth is (5-10):1;

[0050] The rotary evaporation water temperature is 35-45°C and the pH of the PBS buffer solution is 7.4;

[0051] (2) After freeze-drying, adding PBS buffer solution to carry out water bath reaction, and after the reaction, ultrasonic treatment, microporous membrane filtration, and freeze-drying are performed to obtain the nanomaterial of the bifid yeast fermentation product;

[0052] The water bath temperature of the water bath reaction is 35-45° C., the water bath reaction time is 10-20 min, and the pore size of the microporous filter membrane is 0.2 μm.

[0053] Preferably, the composition of the present invention further comprises the following components in parts by weight:

[0054] Solubilizer 0.1-1 part

[0055] 5-15 parts moisturizer

[0056] 1.2-5.5 parts of preservatives

[0057] The solubilizer can be selected from common cosmetic solubilizers on the market. Preferably, the solubilizer is SOLUBILISANT LRI.

[0058] SOLUBILISANT LRI, also known as LRI solubilizer or super-compatibilizer, is a unique surfactant blend with exceptional solubilization properties. It can solubilize fragrances, essential oils, vitamins, and other oily cosmetic ingredients in water, forming a stable microemulsion. SOLUBILISANT LRI's exceptional solubilizing power allows its use in formulations to be significantly reduced compared to other similar products. It is highly safe and has been tested to be non-irritating to skin and eyes. The more complex (mixed) the hydrophobic mixture, the stronger its solubilizing power, leaving a soft, smooth feeling on the skin without a sticky feel, while also allowing the fragrance's aroma to shine through.

[0059] The moisturizer may be selected from commonly available ones on the market, exemplified by glycerin, allantoin, trehalose, and betaine, but not limited thereto.

[0060] Preservatives may be selected from commonly available ones on the market, exemplified by, but not limited to, p-hydroxyacetophenone, 1,3-butanediol, 1,2-pentanediol, and 1,2-hexanediol.

[0061] The present invention also provides a method for preparing the above-mentioned composition, which comprises the following steps: adding ceramide, bifid yeast fermentation product filtrate, and bosine by weight into a reactor, mixing and stirring uniformly, and obtaining the composition.

[0062] The composition or the composition prepared by the preparation method is used in cosmetics.

[0063] Preferably, the cosmetic is a toner, lotion, essence, cream, ointment, mask, gel or freeze-dried powder.

[0064] Example 1

[0065] The anti-aging, firming and soothing composition comprises the following components in parts by weight:

[0066] 2.5 parts of Ceramide AP

[0067] 25 parts of bifid yeast fermentation product

[0068] 5 parts of Bosin

[0069] Example 2

[0070] The anti-aging, firming and soothing composition comprises the following components in parts by weight:

[0071] 2.5 parts of Ceramide NP

[0072] 25 parts of bifid yeast fermentation product

[0073] 5 parts of Bosin

[0074] The preparation process of the bifid yeast fermentation product comprises the following steps:

[0075] (1) PEG5k-PCL2k nanomicelles, bifid yeast fermentation broth, and methanol were mixed to form a mixed liquid, and the mixed liquid was rotary evaporated at a water temperature of 35° C. to form a dry film; wherein the volume ratio of the PEG5k-PCL2k nanomicelles to the bifid yeast fermentation broth was 5:1;

[0076] (2) After freeze-drying, a PBS buffer solution with a pH of 7.4 was added, and the reaction was carried out in a water bath at a water temperature of 35°C. After the reaction was completed for 20 minutes, it was ultrasonically treated, filtered using a microporous filter membrane with a pore size of 0.2 μm, and freeze-dried to obtain the nanomaterial of the bifid yeast fermentation product.

[0077] Example 3

[0078] The anti-aging, firming and soothing composition comprises the following components in parts by weight:

[0079] 2.5 parts of Ceramide EOP

[0080] 7.5 parts of bifid yeast fermentation product

[0081] 10 parts of Bosin

[0082] The preparation process of the bifid yeast fermentation product comprises the following steps:

[0083] (1) PEG5k-PCL2k nanomicelles, bifid yeast fermentation broth, and methanol were mixed to form a mixed liquid, and the mixed liquid was rotary evaporated at a water temperature of 35° C. to form a dry film; wherein the volume ratio of the PEG5k-PCL2k nanomicelles to the bifid yeast fermentation broth was 10:1;

[0084] (2) After freeze-drying, a PBS buffer solution with a pH of 7.4 was added, and the reaction was carried out in a water bath at a water temperature of 35°C. After the reaction was completed for 20 minutes, it was ultrasonically treated, filtered using a microporous filter membrane with a pore size of 0.2 μm, and freeze-dried to obtain the nanomaterial of the bifid yeast fermentation product.

[0085] Example 4

[0086] The anti-aging, firming and soothing composition comprises the following components in parts by weight:

[0087]

[0088] The preparation process of the bifid yeast fermentation product comprises the following steps:

[0089] (1) PEG5k-PCL2k nanomicelles, bifid yeast fermentation broth, and methanol were mixed to form a mixed liquid, and the mixed liquid was rotary evaporated at a water temperature of 35° C. to form a dry film; wherein the volume ratio of the PEG5k-PCL2k nanomicelles to the bifid yeast fermentation broth was 10:1;

[0090] (2) After freeze-drying, a PBS buffer solution with a pH of 7.4 was added, and the reaction was carried out in a water bath at a water temperature of 35°C. After the reaction was completed for 20 minutes, it was ultrasonically treated, filtered using a microporous filter membrane with a pore size of 0.2 μm, and freeze-dried to obtain the nanomaterial of the bifid yeast fermentation product.

[0091] Example 5

[0092] The anti-aging, firming and soothing composition comprises the following components in parts by weight:

[0093] 1 part of Ceramide NS

[0094] 0.1 parts of filtrate of bifid yeast fermentation product

[0095] 0.1 parts of Bose

[0096] The preparation process of the bifid yeast fermentation product comprises the following steps:

[0097] (1) PEG5k-PCL2k nanomicelles, bifid yeast fermentation broth, and methanol were mixed to form a mixed liquid, and the mixed liquid was rotary evaporated at a water temperature of 35° C. to form a dry film; wherein the volume ratio of the PEG5k-PCL2k nanomicelles to the bifid yeast fermentation broth was 10:1;

[0098] (2) After freeze-drying, a PBS buffer solution with a pH of 7.4 was added, and the reaction was carried out in a water bath at a water temperature of 35°C. After the reaction was completed for 20 minutes, it was ultrasonically treated, filtered using a microporous filter membrane with a pore size of 0.2 μm, and freeze-dried to obtain the nanomaterial of the bifid yeast fermentation product.

[0099] Comparative Example 1

[0100] The anti-aging, firming and soothing composition comprises the following components in parts by weight:

[0101] 5 parts of Ceramide NP

[0102] 10 parts of Bosin

[0103] Comparative Example 2

[0104] The anti-aging, firming and soothing composition comprises the following components in parts by weight:

[0105] 50 parts of filtrate of bifid yeast fermentation product

[0106] 10 parts of Bosin

[0107] Comparative Example 3

[0108] The anti-aging, firming and soothing composition comprises the following components in parts by weight:

[0109] 5 parts of Ceramide NP

[0110] 50 parts of filtrate of bifid yeast fermentation product

[0111] Performance Testing

[0112] Stability test: The anti-aging, firming and soothing compositions in Examples 1-5 and Comparative Examples 1-3 were placed in a 40°C environment for 48 hours, and the state of the anti-aging, firming and soothing compositions was observed after returning to room temperature. Subsequently, the anti-aging, firming and soothing compositions were placed in a -8°C environment for 48 hours, and the state of the anti-aging, firming and soothing compositions was observed after returning to room temperature. The test results are shown in Table 1.

[0113] Table 1 Performance test results

[0114]

[0115]

[0116] From the above results, it can be seen that in the present invention, the bifid yeast fermentation product formed by the bifid yeast fermentation broth formed by using ceramide, bifid yeast fermentation product and bosine as the main active ingredients of the composition can effectively improve its dispersibility and stability in the composition and is not easy to stratify and precipitate.

[0117] Examples 1-5 and Comparative Examples 1-3 were tested for DPPH free radical scavenging ability.

[0118] DPPH free radical scavenging ability detection method:

[0119] (1) Take 4 mL DPPH solution (weigh DPPH, dilute to 250mL with anhydrous ethanol, and prepare DPPH solution) and 1 mL 95% ethanol, mix well for reaction, and measure the absorbance A0 at 515 nm with 95% ethanol as reference.

[0120] (2) 4 mL of 95% ethanol and 1 mL of the composition prepared in Example 19 and Comparative Example 14 were mixed and reacted. The absorbance value Ax was measured at 515 nm using 95% ethanol as a reference.

[0121] (3)3) Take 4 mL 1 mL of DPPH solution and Example and comparative example The prepared composition was mixed and reacted, and the absorbance value Ay was measured at 515 nm with 95% ethanol as a reference.

[0122] The test results are shown in the following table:

[0123] Table 2 DPPH free radical scavenging rate

[0124]

[0125]

[0126] As can be seen from the above table, the compositions prepared in Examples 1-5 of the present invention have a scavenging efficiency of 85-94% on DPPH free radicals, among which Example 3 has the best antioxidant effect.

[0127] Examples 1-5 and Comparative Examples 1-3 were tested for cell repair performance.

[0128] Fix performance test

[0129] Stimulate fibroblast proliferation

[0130] Test method:

[0131] Cells in the logarithmic growth phase were selected, collected after trypsin digestion, and prepared into a cell suspension, which was counted using a cell counter. The cell suspension was inoculated into two 96-well plates and cultured. After the inoculation, the cells were incubated at 37°C, 5% CO2 for 24±2 hours. The culture medium was discarded, and the blank control group (containing DMEM culture medium) and the test sample group (containing DMEM culture medium with a sample concentration of 0.1 mg / ml) were dosed, with 100 μL per well in a 37°C, 5% CO2 incubator for 24 hours. The cell growth status was observed under a biological microscope and photographed for record. 24±2 hours after the administration, the plates were removed for MTT test, and the absorbance (492 nm) of each group was measured.

[0132] Cell proliferation rate = (OD value of test group + average OD value of blank control group after 24 hours) / average OD value of blank control group after 24 hours * 100%

[0133] Table 3 Test results of stimulating fibroblast proliferation

[0134] serial number 24h appreciation rate (%) Example 1 3.72 Example 2 3.81 Example 3 4.12 Example 4 4.15 Example 5 4.01 Comparative Example 1 2.15 Comparative Example 2 2.54 Comparative Example 3 2.41

[0135] In summary, bosera can improve skin elasticity and reduce wrinkles; ceramide and bifid yeast fermentation product filtrate can repair the skin barrier.

[0136] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. An anti-aging, firming and soothing composition, characterized in that: The composition comprises the following components in parts by weight: Ceramide 0.002-5 parts 0.1-50 parts of bifid yeast fermentation product 0.1-10 parts of Bose The ceramide includes at least one of ceramide AP, ceramide NP, ceramide EOP, ceramide AS, ceramide NS, and ceramide NG.

2. The anti-aging, firming and soothing composition according to claim 1, characterized in that: The weight ratio of the ceramide, the bifid yeast fermentation product and bosine is 1:(1-3):(3-5).

3. The anti-aging, firming and soothing composition according to claim 2, characterized in that: The bifid yeast fermentation product is a bifid yeast fermentation product nanomaterial formed by bifid yeast fermentation liquid loaded with PEG-PCL, and the average particle size of the bifid yeast fermentation product nanomaterial is 130-160 nm.

4. The anti-aging, firming and soothing composition according to claim 3, characterized in that: The average molecular weight of PEG used to synthesize the PEG-PCL is 5 kDa, and the average molecular weight of PCL used to synthesize the PEG-PCL is 2 kDa.

5. The anti-aging, firming and soothing composition according to claim 3 or 4, characterized in that: The method for preparing the nanomaterial of the bifid yeast fermentation product comprises the following steps: (1) PEG-PCL nanomicelles, bifid yeast fermentation broth, and methanol are mixed to form a mixed liquid, and a dry film is formed after rotary evaporation; wherein the volume ratio of the PEG-PCL nanomicelles to the bifid yeast fermentation broth is (5-10):1; (2) After freeze-drying, PBS buffer solution is added to carry out water bath reaction, and after the reaction is completed, ultrasonic treatment, microporous membrane filtration, and freeze-drying are performed to obtain the nanomaterial of the bifid yeast fermentation product.

6. The anti-aging, firming and soothing composition according to claim 4, characterized in that: The water temperature of the rotary evaporation in step (1) is 35-45°C; In the step (2), the pH of the PBS buffer solution is 7.4, the water bath temperature of the water bath reaction is 35-45° C., the water bath reaction time is 10-20 min, and the pore size of the microporous filter membrane is 0.2 μm.

7. The anti-aging, firming and soothing composition according to claim 1, characterized in that: Also includes the following components in parts by weight: Solubilizer 0.1-1 part 5-15 parts moisturizer 1.2-5.5 parts of preservatives The solubilizing agent is SOLUBILISANT LRI; The moisturizing agent includes one or more of glycerin, allantoin, trehalose, and betaine; The preservative includes one or more of p-hydroxyacetophenone, 1,3-butanediol, 1,2-pentanediol, and 1,2-hexanediol.

8. A method for preparing the anti-aging, firming and soothing composition according to any one of claims 1 to 7, characterized in that: The following steps are involved: Add ceramide, bifid yeast fermentation product and bosine into a reactor according to weight parts, mix and stir evenly to obtain the product.

9. Use of the anti-aging, firming and soothing composition according to any one of claims 1 to 7 in cosmetics.

10. The use according to claim 9, characterized in that The cosmetics are toner, lotion, essence, cream, ointment, facial mask, gel or freeze-dried powder.

Images