Application of composition in preparation of product for regulating spleen deficiency type intestinal flora

Abstract

The invention relates to application of a composition in preparation of a product for regulating spleen deficiency type intestinal flora. Tests prove that the composition disclosed by the invention can be used for improving intestinal flora disorder caused by spleen deficiency. Therefore, the composition disclosed by the invention has the effect of regulating the spleen-deficiency type intestinal flora and can be used for preparing products for regulating the spleen-deficiency type intestinal flora.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine compositions and relates to an application of a composition in preparing a product for regulating spleen deficiency type intestinal flora. Background Art

[0002] "Root spleen deficiency" is a syndrome in Traditional Chinese Medicine (TCM) characterized by fatigue, loss of appetite, weight loss, and dry, falling hair. Cheng Yue and others from Liaoning University of Traditional Chinese Medicine published a paper titled "Exploring the Essential Traditional Chinese Medicine Essence of the Intestinal Microbiome Based on the Theory of Spleen and Stomach" in Shizhen Traditional Chinese Medicine. The paper states that "effective spleen function nourishes the microbiome and maintains a healthy body," and that "dysfunction of the spleen function is often accompanied by disturbances in the intestinal microbiome." This theory is also supported by the paper "Effects of Jianpi Zhixie Granules on the Microbiome of Mice Modeling Spleen Deficiency and Antibiotic-Induced Intestinal Dysbiosis," authored by Li Qiuming and others from Changchun University of TCM and published in the Chinese Journal of Basic Traditional Chinese Medicine.

[0003] Intestinal dysbiosis refers to an imbalance caused by changes in the composition, activity, or distribution of the intestinal flora. Dysbiosis is a common clinical condition that can occur in people of all ages, but is more common in the elderly and infants. This imbalance can be caused by a variety of factors, including digestive system diseases, metabolic diseases (such as hyperglycemia / hyperlipidemia), anti-infective medications (especially broad-spectrum anti-infectives), and an unhealthy diet. However, currently, many researchers developing products to improve intestinal dysbiosis primarily use antibiotic-induced models, while also relying on various pathologically induced bacterial disturbances as efficacy evaluation indicators, such as loperamide-induced models, alcohol-induced models, and inflammatory bowel disease-induced mouse models. However, these products have not yet been reported for treating spleen deficiency-related intestinal dysbiosis, and to date, there are virtually no products designed to regulate spleen deficiency-related dysbiosis.

[0004] In view of this, developing a product that can effectively regulate the intestinal flora of spleen deficiency has become an urgent problem to be solved by those skilled in the art. Summary of the Invention

[0005] In order to solve the problems existing in the prior art, the present invention provides an application of a composition in the preparation of a product for regulating intestinal flora of spleen deficiency type. The technical solution adopted by the present invention is as follows:

[0006] The invention discloses an application of a composition in preparing a product for regulating intestinal flora of spleen deficiency type. The composition comprises the following raw materials: 33.7 wt% of pumpkin peel, 20.8 wt% of pumpkin pedicle, 12.9 wt% of pumpkin vein, 7.9 wt% of Poria cocos, 9.0 wt% of Tremella fuciformis and 15.7 wt% of xylo-oligosaccharide.

[0007] In a preferred embodiment of the present invention, the regulation of the spleen deficiency type intestinal flora includes improving the composition structure of the spleen deficiency type intestinal flora, and further includes shortening the sample distance of the intestinal flora between the healthy group and the healthy group, and reducing the species difference between the healthy group and the healthy group.

[0008] In a preferred embodiment of the present invention, the regulation of spleen deficiency type intestinal flora includes, at the family classification level,

[0009] Increased abundance of the following fungal families:

[0010] Eubacterium_coprostanoligenes_group; and,

[0011] Bacteroidales_BS11_gut_group; and,

[0012] Tannerellaceae; and,

[0013] Selenomonadaceae; and,

[0014] p-2534-18B5_gut_group;

[0015] and / or,

[0016] Reduced abundance of the following fungal families:

[0017] Campylobacteraceae.

[0018] In a preferred embodiment of the present invention, the regulation of spleen deficiency type intestinal flora includes, at the species classification level,

[0019] Increases the abundance of the following bacterial species:

[0020] uncultured_rumen_bacterium_f_Eubacterium_coprostanoligenes_group; and,

[0021] Porphyromonadaceae_bacterium_DJF_B175; and,

[0022] uncultured_bacterium_g_Alistipes; and,

[0023] uncultured_bacterium_f_p-2534-18B5_gut_group; and,

[0024] uncultured_Clostridiales_bacterium_g_Monoglobus; and,

[0025] uncultured_bacterium_f_Oscillospiraceae;

[0026] and / or,

[0027] Reduce the abundance of the following bacterial species:

[0028] Ruminococcus_sp._N15.MGS-57_g_Ruminococcus.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The composition of the present invention has been shown to regulate the intestinal flora disorder caused by spleen deficiency (adjusting the flora structure to a level close to normal, increasing the abundance of beneficial bacteria and reducing the abundance of harmful bacteria at the family level, and increasing the abundance of beneficial bacteria and reducing the abundance of harmful bacteria at the species level) in the first aspect, and improving the spleen deficiency symptoms of spleen deficiency mice (increasing the daily food intake of spleen deficiency mice, increasing the weight of spleen deficiency mice, increasing the autonomous activity ability of spleen deficiency mice, and improving the messy / dull / yellowing hair of spleen deficiency mice). Therefore, the composition of the present invention has the effect of regulating spleen deficiency type intestinal flora and can be used to prepare a product for regulating spleen deficiency type intestinal flora. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 is the dilution curve of intestinal flora in each group of fecal samples of the present invention;

[0032] Figure 2 Schematic diagram of PCoA of intestinal flora in each group of the present invention at the OTU level;

[0033] Figure 3 The microbial community composition diagram (species level) of each group of test animals in the present invention;

[0034] Figure 4 Schematic diagram of the significance analysis of species differences in the intestinal flora of the test animals in each group of the present invention (family level);

[0035] Figure 5 Schematic diagram of the significance analysis of intestinal flora species differences among the test animals in each group of the present invention (species level);

[0036] Figure 6 Schematic diagram of the statistical analysis of the hair condition scores of the test animals in each group of the present invention;

[0037] Figure 7This is a statistical analysis diagram of the average daily food intake of the test animals in each group of the present invention in the third week;

[0038] Figure 8 Schematic diagram of statistical analysis of weight changes of test animals in each group before and after the experiment;

[0039] Figure 9 Schematic diagram of statistical analysis of the evaluation of autonomous activity of each group of test animals in the present invention;

[0040] Figure 10 These are photos of the behavioral status of the test animals in each group of the present invention;

[0041] In the figure, CON represents the blank group, MOD represents the model group, and JTTC represents the combination group. DETAILED DESCRIPTION

[0042] The present invention will be described in detail below with reference to specific drawings and embodiments. It is necessary to point out that the following embodiments are only used to further illustrate the present invention and cannot be understood as limiting the scope of protection of the present invention. Some non-essential improvements and adjustments made by those skilled in the art to the present invention based on the contents of the present invention still fall within the scope of protection of the present invention.

[0043] The raw materials used in the examples are all conventional commercially available raw materials. Among them, the manufacturer of rhubarb medicinal material is Bozhou Baichuan Pharmaceutical Co., Ltd.

[0044] In the present invention, the composition comprises the following weight proportions: 33.7 wt% pumpkin peel, 20.8 wt% pumpkin pedicle, 12.9 wt% pumpkin vein, 7.9 wt% poria cocos, 9.0 wt% tremella fuciformis, and 15.7 wt% xylo-oligosaccharide. The preparation method comprises the following steps: selecting pumpkin peel, pumpkin pedicle, pumpkin vein, poria cocos, and tremella fuciformis according to weight proportion, selecting and removing impurities, and soaking the raw materials in water; decocting the raw materials three times for 30 minutes each time, combining the three decoctions, and filtering the mixture; concentrating the mixture until it becomes a thick paste; and then drying the mixture at low temperature to reduce the moisture content to ≤8% and the ash content to ≤5%. After testing to meet the requirements, the mixture is ultra-finely ground using a jet mill to finally be ground into 500 mesh; and after the grinding is completed, the mixture is thoroughly mixed with xylo-oligosaccharide to obtain the composition. It should be noted that the composition of the present invention is basically the same as the composition in the Chinese invention patent "Capsule formula and preparation method for treating constipation prepared with pumpkin as the main raw material" (publication number: CN1533805A), with the only difference being that aloe vera is not added. The other components and the corresponding preparation methods are exactly the same, and it is actually Jianfang Tiantian Capsule (JTTC).

[0045] In this paper, the dilution curve directly reflects the rationality of the sequencing data volume. When the curve tends to be flat, it indicates that the sequencing data volume is gradually becoming reasonable, and more data will only generate a small number of new species (OTUs). The dilution curve is constructed by comparing the number of sequences extracted and the number of OTUs they can represent.

[0046] The instruments used in the examples are all conventional commercially available instruments.

[0047] Example 1

[0048] 1. Preparation of test samples

[0049] 1.1 Rhubarb decoction: Take 250g of raw rhubarb, add 2000ml of water, soak for 0.5h, simmer for 15min, cool naturally, filter through 4 layers of gauze, combine the decoction, and use a rotary evaporator to concentrate to 250ml, equivalent to 1g / ml of the raw drug.

[0050] 1.2 Composition test sample: Weigh 1 g of the composition and dissolve it in 10 mL of normal saline to obtain a composition test sample with a composition concentration of 0.1 g / mL.

[0051] 2. Animal Grouping, Modeling, and Drug Administration

[0052] 2.1 Experimental animals and animal groups

[0053] The test animals used were mice, and their strain was KM mice (SPF grade). All test animals were of clean grade, and their certificate number was SCXK (Beijing) 2021-0011, with a total of 30 animals. The test animals were all raised in cages, with 5 animals per cage, and males and females were raised in separate cages. The room temperature was maintained at around 24°C, the relative humidity was 40-60%, and the photoperiod was 12h. Ordinary feed was given throughout the experiment, and drinking water was free. The mice were adaptively fed for 1 week, weighed, and randomly divided into 3 groups according to their body weight. Afterwards, modeling and medication were carried out. The modeling method referred to "The Effect of Guiqi Baizhu Decoction on the Expression of Immune Factors and Water Channel Proteins in Mice with Spleen Deficiency" (published in Shizhen Traditional Chinese Medicine and Pharmacy by Gansu University of Chinese Medicine).

[0054] Table 1 Test animals and animal groups

[0055] Group Test animals gender weight Number of animals Blank group KM mice Half male and half female (20±2)g 10 Model Group KM mice Half male and half female (20±2)g 10 Combination group KM mice Half male and half female (20±2)g 10

[0056] 2.2 Animal modeling and drug administration

[0057] Table 2 Modeling and drug administration of test animals

[0058]

[0059] 3 Animal testing

[0060] 3.1 Record of physical signs of test animals in each group

[0061] During the experiment, animal weight was recorded daily. During the third week, the hair condition, behavioral status, food intake, and body weight of each group of test animals were recorded. Furthermore, for the behavioral evaluation of each group of test animals (i.e., evaluation of animal locomotor activity), each group of test animals was placed in an locomotor activity recorder daily, and the number of activity events per unit time was counted.

[0062] The animal hair scoring was based on the Expert Consensus on Diagnosis and Treatment of Spleen Deficiency Syndrome in Traditional Chinese Medicine (2023) and Methodology of Experimental Animal Models in Traditional Chinese Medicine. The external signs of spleen deficiency mice were scored according to the following criteria: ① Hair condition: 0 points for smooth, shiny, non-yellowing, and non-piloerection hair; 1 point for messy, dull, slightly yellowing, and non-piloerection hair; 2 points for messy, dirty, dry, yellowing, and non-piloerection hair; 3 points for messy, dirty, dry, yellowing, and erection hair.

[0063] 3.2 Intestinal flora of test animals in each group (16S rRNA sequencing analysis)

[0064] After two weeks of continuous dosing, the intestinal microbiota of mice in each group was analyzed. Fecal samples were collected from each animal and sent to Shanghai Meiji Biopharmaceutical Technology Co., Ltd. for microbial analysis. The species composition and richness of the microbial communities in each group were analyzed using the online data analysis platform provided by Meiji Biopharmaceuticals.

[0065] 4 Results Analysis

[0066] 4.1 Intestinal flora of test animals in each group

[0067] Figure 1 The intestinal flora dilution curves of the test animals in each group of the present invention are shown. Figure 1 It can be seen that the Shannon-curves have entered a plateau period, indicating that the amount of sequencing data is sufficient to cover all groups, reflect the vast majority of microbial information, and meet the analysis requirements.

[0068] 4.1.1 Distance between intestinal flora samples of each group of test animals .

[0069] Figure 2 The PCoA analysis based on the OTU level is shown to show the sample distance of the intestinal flora of each group of test animals and measure the similarity of microbial composition between groups; the X-axis and Y-axis represent two selected main coordinate axes, and the percentage represents the explanatory value of the main coordinate axis for the difference in sample composition; the scales of the X-axis and Y-axis are relative distances and have no practical meaning; points of different colors or shapes represent samples of different groups. The closer the two sample points are, the more similar the species composition of the two samples is. Figure 2The model group was completely separated from the blank group (P < 0.01), indicating that the composition of the intestinal flora of spleen-deficient mice was significantly altered. The combination group was completely separated from the model group (P < 0.01) and close to the blank group, indicating that the combination intervention regulated the flora composition of spleen-deficient mice, returning their intestinal flora composition to normal.

[0070] 4.1.2 Microbial community composition and differences in the intestinal flora of each group of test animals .

[0071] Figure 3 The microbial community composition diagram (relative abundance) of each group of test animals at the species level is shown. Figure 3 It can be seen that at the family level, the eight species with the highest proportion are: species under Muribaculaceae (uncultured_bacterium_f_Muribaculaceae), species under Eubacteriaceae (uncultured_bacterium_f_Eubacteriaceae), species under ClostridiaUCG-014 (unclassified_g_norank_o_Clostridia_UCG-014), species under Muribaculaceae (unclassified_g_norank_f_Muribaculaceae),

[0072] uncultured_bacterium_g_Rikenellaceae_RC9_gut_group, unclassified_f_Lachnospiraceae, uncultured rumenbacterium o ClostridiaUCG-014, unclassified_f_Prevotellaceae.

[0073] Compared with the blank group, the relative abundances of uncultured_bacterium_f_Muribaculaceae, uncultured_bacterium_f_Eubacteriaceae, unclassified_g_norank_o_Clostridia_UCG-014, and unclassified_f_Lachnospiraceae in the model group increased; Compared with the model group, the relative abundance of uncultured_bacterium_f_Muribaculaceae, uncultured_bacterium_f_Eubacteriaceae, unclassified_g_norank_o_Clostridia_UCG-014, and unclassified_f_Lachnospiraceae in the combination group decreased. Compared with the blank group, the relative abundance of uncultured_bacterium_f_Muribaculaceae and uncultured_bacterium_g_Rikenellaceae_RC9_gut_group in the model group decreased; compared with the model group, the relative abundance of uncultured_bacterium_f_Muribaculaceae,

[0074] The relative abundance of uncultured_bacterium_g_Rikenellaceae_RC9_gut_group increased.

[0075] This shows that the microbial community composition (species level) of spleen deficiency mice has changed significantly. After 2 weeks of intervention with the composition, the microbial community composition of spleen deficiency mice was regulated, and the microbial community composition of the mice returned to normal.

[0076] 4.1.3 Analysis of the significance of species differences in the intestinal flora of the test animals in each group .

[0077] 4.1.3.1 Analysis of the significance of species differences in the intestinal flora of each group of test animals (family level).

[0078] Figure 4The figure shows the schematic diagram of the significant difference analysis of species at the family level in the intestinal flora of each group of test animals. Figure 4 It can be seen that compared with the blank group, the abundance of 5 beneficial bacteria was significantly reduced (P < 0.05), and the abundance of 1 harmful bacteria was significantly increased (P < 0.05). Among them, the 5 beneficial bacteria are:

[0079] The Eubacterium_coprostanoligenes_group, Bacteroidales_BS11_gut_group, Tannerellaceae, Selenomonadaceae, and p-2534-18B5_gut_group were included; one harmful bacterium, Campylobacteraceae, was also included. Among them, the Bacteroidales_BS11_gut_group participates in the fermentation of monosaccharides in the intestine and produces short-chain fatty acids (SCFAs); Tannerellaceae helps suppress intestinal inflammation; and Selenomonadaceae helps enhance immune function. Compared with the model group, the abundance of all five beneficial bacteria in the combination group was significantly increased (P < 0.05), while the abundance of one harmful bacterium was extremely significantly decreased (P < 0.01).

[0080] This indicates that, at the taxonomic level, the abundance of multiple beneficial bacteria in the intestinal flora of spleen-deficient mice was significantly reduced, while the abundance of harmful bacteria was extremely significantly increased. Two weeks of intervention with the combination significantly reversed the imbalance in the intestinal flora of spleen-deficient mice, which had previously been characterized by a significant decrease in the abundance of multiple beneficial bacteria and an extremely significant increase in the abundance of harmful bacteria. This imbalance led to a significant increase in the abundance of multiple beneficial bacteria and a significant decrease in the abundance of harmful bacteria, essentially returning to normal levels.

[0081] 4.1.3.2 Analysis of the significance of species differences in the intestinal flora of each group of test animals (species level).

[0082] Figure 5 The figure shows the schematic diagram of the species difference analysis of the intestinal flora of each group of test animals in the present invention (species level). Figure 5 It can be seen that compared with the blank group, the abundance of the six beneficial bacteria was significantly reduced (P < 0.05), and the abundance of one harmful bacteria was extremely significantly increased (P < 0.01). Among them, the six beneficial bacteria were: uncultured_rumen_bacterium_f_Eubacterium_coprostanoligenes_group, Porphyromonadaceae_bacterium_DJF_B175, another genus of the genus Alistipes (uncultured_bacterium_g_Alistipes),

[0083] uncultured_bacterium_f_p-2534-18B5_gut_group、

[0084] uncultured_Clostridiales_bacterium_g_Monoglobus, uncultured_bacterium_f_Oscillospiraceae; 1 harmful bacteria:

[0085] Ruminococcus_sp._N15.MGS-57_g_Ruminococcus. Among them, uncultured_rumen_bacterium_f_Eubacterium_coprostanoligenes_group is a beneficial bacteria that produces short-chain fatty acids; uncultured_bacterium_g_Alistipes is beneficial for regulating intestinal inflammatory responses; and uncultured_bacterium_f_Oscillospiraceae helps regulate intestinal metabolic levels. Compared with the model group, the abundance of all six beneficial bacteria in the combination group was significantly increased (P < 0.05), while the abundance of one harmful bacteria was extremely significantly decreased (P < 0.01).

[0086] This indicates that, at the species level, the abundance of multiple beneficial bacteria in the intestinal flora of spleen-deficient mice was significantly reduced, while the abundance of harmful bacteria was extremely significantly increased. Two weeks of intervention with the combination significantly reversed the imbalance in the intestinal flora of spleen-deficient mice, which had previously been characterized by a significant decrease in the abundance of multiple beneficial bacteria and an extremely significant increase in the abundance of harmful bacteria. This imbalance led to a significant increase in the abundance of multiple beneficial bacteria and a significant decrease in the abundance of harmful bacteria, essentially returning to normal levels.

[0087] 4.2 Normalized physical signs of test animals in each group

[0088] It was mentioned above that "during the third week, the appearance, behavior, food intake and body weight of each group of mice were recorded daily". The hair condition of each group of mice was scored according to the above scoring criteria, and statistical analysis was performed using GraphPad Prism8.0 software after scoring. The final data were expressed as mean ± standard deviation (one-way analysis of variance was used for comparison between multiple groups); similarly, the behavior, food intake and body weight of each group of mice were also statistically analyzed using GraphPad Prism 8.0 software (one-way analysis of variance was used for comparison between multiple groups). The results are as follows Figures 6 to 9 shown. Figures 6 to 9In the table, # indicates that there is a significant difference between the group and the blank group (P < 0.05), ## indicates that there is an extremely significant difference between the group and the blank group (P < 0.01); * indicates that there is a significant difference between the group and the model group (P < 0.05), ** indicates that there is an extremely significant difference between the group and the model group (P < 0.01).

[0089] Figure 6 The figure shows the statistical analysis of the hair condition scores of each group of test animals in the third week. Figure 6 It can be seen that most of the test animals in the blank group had smooth, shiny, and non-yellowing hair. Compared with the blank group, some of the test animals in the model group had messy, dull, and slightly yellow hair, some had dirty, dry, and yellow hair, and some test animals even had erect hair. Regarding the hair condition score, there was a very significant difference between the model group and the blank group (P<0.01). Compared with the model group, the test animals in the combination group also had messy, dull, and slightly yellow hair at the end of the first week, but after the end of the experiment, the above-mentioned hair condition was significantly improved. Except for a few test animals that still had messy and yellow hair, the hair condition of the other test animals was basically the same as that of the blank group. Regarding the hair condition score, there was a significant difference between the combination group and the model group (P<0.05). This shows that the hair of spleen deficiency mice is messy, dull, yellow, dirty, and dry. After two weeks of intervention with the composition, the "messy, dull, yellow, dirty, and dry hair" of spleen-deficient mice was significantly reversed, and the mice's hair basically returned to being smooth, shiny, and non-yellowing.

[0090] Figure 7 The figure shows the average daily food intake of each group of test animals in the third week. Figure 7 It can be seen that compared with the blank group, the average daily food intake of the test animals in the model group was extremely significantly reduced (P<0.01). Compared with the model group, the test animals in the combination group also showed a decrease in average daily food intake at the end of the first week, but after the end of the experiment, the average daily food intake of the test animals in the combination group was extremely significantly increased (P<0.01). This shows that the average daily food intake of mice with spleen deficiency was significantly reduced. After 2 weeks of intervention with the combination, the average daily food intake of mice with spleen deficiency was significantly increased.

[0091] Figure 8 The figure shows the changes in body weight of each group of animals before and after the experiment. Figure 8 It can be seen that the weight gain of the test animals in the model group was significantly lower than that in the blank group (P<0.05). The weight gain of the test animals in the combination group during the experiment was significantly higher than that in the model group (P<0.05), and was similar to that in the blank group. This shows that the weight gain rate of spleen deficiency mice was significantly slowed down. After 2 weeks of intervention with the combination, the weight gain of spleen deficiency mice was significantly increased, and it was basically restored to the level of the blank group.

[0092] Figure 9 The figure shows the evaluation of the autonomous activities of the test animals in each group, specifically the number of activities of the mice within 5 minutes. Figure 9 It can be seen that compared with the blank group, the number of activities of the model group animals within 5 minutes was extremely significantly reduced (P < 0.01). Compared with the model group, the number of activities of the combination group was significantly increased (P < 0.05), basically returning to the level of the blank group. This shows that the number of activities per unit time in spleen deficiency mice was significantly reduced. After 2 weeks of intervention with the combination, the number of activities per unit time in spleen deficiency mice was significantly increased, basically returning to the level of the blank group.

[0093] besides, Figure 10 The following pictures show the behavior status of the test animals in each group, which were taken on the first day after the end of the experiment. Figure 10 It can be seen that the mice in the blank group had normal activities; the mice in the model group were tired, lazy, and liked to gather in groups, which proved that the mice in the model group had significant symptoms of spleen deficiency; after 2 weeks of intervention with the composition, the mice's behavior of "tiredness, laziness, and like to gather in groups" was significantly improved.

Claims

1. A composition for preparing a product for regulating intestinal flora of spleen deficiency type, wherein the composition comprises the following raw materials: 33.7 wt% of pumpkin peel, 20.8 wt% of pumpkin pedicle, 12.9 wt% of pumpkin vein, 7.9 wt% of Poria cocos, 9.0 wt% of Tremella fuciformis and 15.7 wt% of xylo-oligosaccharide.

2. The use according to claim 1, characterized in that: The regulating of the spleen deficiency type intestinal flora includes improving the composition structure of the spleen deficiency type intestinal flora, and further includes shortening the sample distance of the intestinal flora between the healthy group and the healthy group, and reducing the species difference between the healthy group and the healthy group.

3. The use according to claim 1, characterized in that: The said regulation of spleen deficiency type intestinal flora includes, at the family classification level, Increased abundance of the following fungal families: Eubacterium_coprostanoligenes_group; and, Bacteroidales_BS11_gut_group; and, Tannerellaceae; and, Selenomonadaceae; and, p-2534-18B5_gut_group; and / or, Reduced abundance of the following fungal families: Campylobacteraceae.

4. The use according to claim 1, characterized in that: The regulation of spleen deficiency type intestinal flora includes, at the species classification level, Increases the abundance of the following bacterial species: uncultured_rumen_bacterium_f_Eubacterium_coprostanoligenes_group; and, Porphyromonadaceae_bacterium_DJF_B175; and, uncultured_bacterium_g_Alistipes; and, uncultured_bacterium_f_p-2534-18B5_gut_group; and, uncultured_Clostridiales_bacterium_g_Monoglobus; and, uncultured_bacterium_f_Oscillospiraceae; and / or, Reduce the abundance of the following bacterial species: Ruminococcus_sp._N15.MGS-57_g_Ruminococcus.

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