Rice nano bacterial fertilizer for reducing incidence rate of rice blast

Abstract

The invention belongs to the technical field of microbial fertilizer manufacturing, and particularly relates to a rice nano bacterial fertilizer for reducing the incidence rate of rice blast. According to the nano bacterial fertilizer, corynespora cassiicola, coniothyrium minitans YAFEF037 and mucor are introduced, antibacterial substances can be generated, growth and reproduction of pyricularia oryzae can be inhibited, competitive advantages can be formed on rhizosphere and leaf surfaces of rice, ecological niches of pathogenic bacteria are occupied, and then the incidence rate of the pyricularia oryzae is effectively reduced; due to the high specific surface area and adsorbability of the nano silicon dioxide, a good adhesion and growth environment is provided for microorganisms, and the metabolic capability and stress resistance of the strain are further enhanced; the organic matter fermentation liquor contains a large amount of nutritional ingredients, growth and development of rice root systems can be promoted, and the nutrient absorption capacity of rice is improved; the growth vigor and the stress resistance of the rice are improved through the nano bacterial fertilizer, the resistance of the rice to the rice blast is further enhanced, and a synergistic effect mechanism of promoting the fertilizer with bacteria and preventing diseases with the fertilizer is formed.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial fertilizer manufacturing, and particularly relates to a rice nano-fertilizer capable of reducing the incidence of rice blast. Background Art

[0002] Currently, rice blast control relies primarily on chemical pesticides. However, frequent use of chemical pesticides can easily lead to the development of resistance in the rice blast fungus, resulting in a gradual decline in pesticide effectiveness. This necessitates increasing dosages or changing pesticide types, further increasing the environmental burden and agricultural production costs. Furthermore, chemical pesticide residues can pollute soil, water, and air, severely damaging the ecological environment and affecting ecological balance. They can also enter the human body through the food chain, potentially harming human health, affecting the quality and safety of agricultural products, and limiting their market competitiveness. Therefore, developing a green, environmentally friendly, and efficient method for controlling rice blast is of great practical significance. Summary of the Invention

[0003] The purpose of the present invention is to overcome the deficiencies of the prior art and develop a rice nanofertilizer with higher activity, greater stability and higher efficiency, which can effectively reduce the incidence of rice blast.

[0004] The invention provides a rice nano-fertilizer for reducing the incidence of rice blast. The nano-fertilizer comprises a fungal solution of Corynespora multilocus, a fungal solution of Conchoderma spp. YAFEF037, a fungal solution of Mucor, nano-silicon dioxide and an organic fermentation solution.

[0005] Preferably, in parts by mass, the nano-fertilizer includes 5-15 parts of multi-main Corynespora fungus liquid, 5-15 parts of Conchoderma YAFEF037 fungus liquid, 5-15 parts of Mucor fungus liquid, 20-30 parts of nano-silicon dioxide and 30-50 parts of organic fermentation liquid.

[0006] Preferably, the number of viable bacteria in the polyhomocybe fungus solution is ≥10×10 8 cfu / mL; the number of viable bacteria in the YAFEF037 bacterial solution of the Shieldia spp. is ≥10×10 8 cfu / mL; the number of viable bacteria in the Mucor culture solution is ≥10×10 8 cfu / mL.

[0007] Preferably, the organic fermentation liquid is obtained by fermenting 30-40 parts of rice straw, 20-30 parts of poultry and livestock manure, 10-20 parts of bone meal, 3-5 parts of wood ash, 350-700 parts of water and 10-20 parts of EM bacterial agent, in parts by mass.

[0008] Preferably, the fermentation condition is 28° C. to 30° C. for 3 to 5 days.

[0009] The present invention also provides a preparation method of the nano-bacterial fertilizer described in the above technical solution, which comprises the following steps: mixing nano-silicon dioxide with water to obtain a nano-silicon dioxide suspension, adjusting the pH of the suspension to 3.0-4.0 so that the surface of the nano-silicon dioxide is positively charged; centrifuging a multi-main spore fungus liquid, a Shield mold YAFEF037 fungus liquid and a Mucor fungus fungus liquid to remove the supernatant, resuspending them with deionized water and then mixing them to obtain a bacterial suspension, adjusting the pH of the bacterial suspension to 9.0-10.0 so that the surface of the bacterial cells is negatively charged; mixing the bacterial suspension with the nano-silicon dioxide suspension, adsorbing them for 1-2 hours to obtain a nano-silicon dioxide fixed bacterial agent; mixing the nano-silicon dioxide fixed bacterial agent with an organic fermentation liquid to obtain a mixture; adding water to the mixture to granulate, and drying to obtain the nano-bacterial fertilizer.

[0010] Preferably, the water content of the nano-fertilizer is 10% to 20%.

[0011] Preferably, the drying temperature is 45°C to 55°C.

[0012] The present invention also provides a method for reducing the incidence of rice blast based on the nano-bacterial fertilizer described in the above technical solution or the nano-bacterial fertilizer prepared by the method described in the above technical solution, the method comprising: performing a first fertilization before rice sowing, performing a second fertilization during the rice bark-forming stage, and performing a third fertilization during the rice booting stage; the fertilizers applied in the first fertilization, the second fertilization, and the third fertilization are all the nano-bacterial fertilizer described in the above technical solution.

[0013] Preferably, the amount of the first fertilization is 10-15 kg / mu; the amount of the second fertilization is 5-10 kg / mu; and the amount of the third fertilization is 5-8 kg / mu.

[0014] Beneficial effects of the present invention: Reducing the incidence of rice blast disease by introducing beneficial microorganisms into the soil The nano-fertilizer of the present invention incorporates the strains of Corynespora multilocus, Cercospora spp. YAFEF037, and Mucor. These strains produce antimicrobial substances that inhibit the growth and reproduction of the rice blast pathogen, thereby effectively reducing the incidence of rice blast. Furthermore, the beneficial microorganisms can form a competitive advantage in the rhizosphere and on the surface of rice leaves, occupying the ecological niche of pathogens and further reducing the chance of infection by the pathogen. The present invention has been shown to reduce the incidence of rice blast to 1.13% to 1.42% after using the nano-fertilizer, significantly outperforming traditional control methods.

[0015] The high specific surface area and adsorption properties of nano-silicon dioxide provide a good attachment and growth environment for microorganisms, further enhancing the metabolic capacity and stress resistance of the bacteria; the organic fermentation broth contains a large amount of organic acids, amino acids, vitamins and other nutrients, which can promote the growth and development of rice roots and improve the rice's ability to absorb nutrients; the present invention uses nano-bacterial fertilizer to improve the growth potential and stress resistance of rice, further enhancing the rice's resistance to rice blast, forming a synergistic mechanism of "using bacteria to promote fertilizer and using fertilizer to prevent disease."

[0016] The nano-fertilizer of the present invention is composed entirely of biological materials and natural organic matter, does not contain any chemical pesticides or harmful substances, and is pollution-free to soil, water, and air, meeting the development requirements of green agriculture. Compared with traditional chemical pesticide control methods, the nano-fertilizer of the present invention does not cause pathogens to develop drug resistance, thus avoiding the potential harm of chemical pesticide residues to the ecological environment and human health, and is a sustainable technology for controlling rice blast.

[0017] The present invention uses nano-silica to immobilize the bacterial strains. The two are tightly bonded through electrostatic adsorption to form a stable nanocomposite structure. This structure effectively protects the bacterial strains from environmental factors such as heat, dryness, and ultraviolet rays, significantly improving their survival rate and activity.

[0018] The preparation method of the invention is simple and easy to implement, has mild operating conditions, does not require complex equipment and high cost investment; and the raw materials are widely available, low in price and easy to obtain. DETAILED DESCRIPTION

[0019] In order to further illustrate the present invention, a rice nano-fertilizer for reducing the incidence of rice blast provided by the present invention is described in detail below in conjunction with examples, but they should not be understood as limiting the scope of protection of the present invention.

[0020] In the examples of the present invention, Corynespora multilocus was purchased from China Center for Type Culture Collection (CCTCC) with a deposit number of CCTCC AF 2022043; Conchoderma spp. YAFEF037 was purchased from China Center for Type Culture Collection (CCTCC) with a deposit number of CCTCC M 2022610; and Mucor was purchased from Mingzhou Biotechnology with a product number of B94706.

[0021] Example 1 Take 30 parts of rice straw, 30 parts of poultry and livestock manure, 20 parts of bone meal, 5 parts of wood ash, 500 parts of water and 10 parts of EM bacteria agent, ferment them at 28°C for 5 days to obtain organic fermentation liquid.

[0022] Take the multi-main coryneform fungus solution (viable bacteria count is 10×10 9cfu / mL) 10 copies, YAFEF037 bacterial solution of Conchoderma lucidum (viable bacteria count 10×10 9 cfu / mL) 10 copies, Mucor culture solution (viable count 10×10 9 cfu / mL), 10 parts of nano-silica and 35 parts of organic fermentation broth were prepared for later use.

[0023] Nano-silica is mixed with water to obtain a nano-silica suspension, and the pH of the suspension is adjusted to 3.7 so that the surface of the nano-silica is positively charged; a fungus liquid of Corynespora multiflora, a fungus liquid of Coryneformis YAFEF037, and a fungus liquid of Mucor are respectively centrifuged to remove the supernatant, resuspended with deionized water, and then mixed to obtain a bacterial suspension, and the pH of the bacterial suspension is adjusted to 9.8 so that the surface of the bacterial body is negatively charged; the bacterial suspension is mixed with the nano-silica suspension, and adsorbed for 1 to 2 hours to obtain a nano-silica fixed bacterial agent; the nano-silica fixed bacterial agent is mixed with an organic matter fermentation liquid to obtain a mixture; water is added to the mixture to granulate, and the mixture is dried to obtain a nano-bacterial fertilizer.

[0024] Example 2 Take 40 parts of rice straw, 30 parts of poultry and livestock manure, 20 parts of bone meal, 4 parts of wood ash, 650 parts of water and 15 parts of EM bacteria agent, ferment them at 28°C for 5 days to obtain organic fermentation liquid.

[0025] Take the multi-main coryneform fungus solution (viable bacteria count is 10×10 9 cfu / mL) 10 copies, YAFEF037 bacterial solution of Conchoderma lucidum (viable bacteria count 10×10 9 cfu / mL) 10 copies, Mucor culture solution (viable count 10×10 9 cfu / mL), 15 parts of nano-silica, 30 parts of organic fermentation broth and 40 parts of organic fermentation broth were prepared for later use.

[0026] Nano-silica is mixed with water to obtain a nano-silica suspension, and the pH of the suspension is adjusted to 4.0 so that the surface of the nano-silica is positively charged; a fungus liquid of Corynespora multiflora, a fungus liquid of Coleopsina spp. YAFEF037 and a fungus liquid of Mucor are respectively centrifuged to remove the supernatant, resuspended with deionized water and then mixed to obtain a bacterial suspension, and the pH of the bacterial suspension is adjusted to 9.5 so that the surface of the bacterial body is negatively charged; the bacterial suspension is mixed with the nano-silica suspension, and adsorbed for 1 to 2 hours to obtain a nano-silica fixed bacterial agent; the nano-silica fixed bacterial agent is mixed with an organic matter fermentation liquid to obtain a mixture; water is added to the mixture to granulate, and the mixture is dried to obtain a nano-bacterial fertilizer.

[0027] Example 3 Take 40 parts of rice straw, 20 parts of poultry and livestock manure, 10 parts of bone meal, 5 parts of wood ash, 700 parts of water and 20 parts of EM bacteria agent, ferment them at 30℃ for 3 days to obtain organic fermentation liquid.

[0028] Take the multi-main coryneform fungus solution (viable bacteria count is 10×10 9 cfu / mL) 15 copies, YAFEF037 bacterial solution of Conchoderma spp. (viable bacteria count 10×10 9 cfu / mL) 15 copies, Mucor culture solution (viable count 10×10 9 cfu / mL), 10 parts of nano-silica and 50 parts of organic fermentation broth were prepared for later use.

[0029] Nano-silica is mixed with water to obtain a nano-silica suspension, and the pH of the suspension is adjusted to 3.5 so that the surface of the nano-silica is positively charged; a fungus liquid of Corynespora multiflora, a fungus liquid of Coleopsina spp. YAFEF037, and a fungus liquid of Mucor are respectively centrifuged to remove the supernatant, resuspended with deionized water, and then mixed to obtain a bacterial suspension, and the pH of the bacterial suspension is adjusted to 10.0 so that the surface of the bacterial body is negatively charged; the bacterial suspension is mixed with the nano-silica suspension, and adsorbed for 1 to 2 hours to obtain a nano-silica fixed bacterial agent; the nano-silica fixed bacterial agent is mixed with an organic matter fermentation liquid to obtain a mixture; water is added to the mixture to granulate, and the mixture is dried to obtain a nano-bacterial fertilizer.

[0030] Comparative Example 1 The difference from Example 1 is that nano-silicon dioxide is not used, specifically as follows: Take 30 parts of rice straw, 30 parts of poultry and livestock manure, 20 parts of bone meal, 5 parts of wood ash, 500 parts of water and 10 parts of EM bacteria agent, ferment them at 28°C for 5 days to obtain organic fermentation liquid.

[0031] Take the multi-main coryneform fungus solution (viable bacteria count is 10×10 9 cfu / mL) 10 copies, YAFEF037 bacterial solution of Conchoderma lucidum (viable bacteria count 10×10 9 cfu / mL) 10 copies, Mucor culture solution (viable count 10×10 9 cfu / mL), 10 parts of silica, and 35 parts of organic fermentation liquid were evenly mixed to obtain a mixture; water was added to the mixture to granulate, and the mixture was dried to obtain a nano-bacterial fertilizer.

[0032] Comparative Example 2 The difference from Example 1 is that the polysporus fungus solution is not used, and the specific steps are as follows: Take 30 parts of rice straw, 30 parts of poultry and livestock manure, 20 parts of bone meal, 5 parts of wood ash, 500 parts of water and 10 parts of EM bacteria agent, ferment them at 28°C for 5 days to obtain organic fermentation liquid.

[0033] Take the bacterial liquid of YAFEF037 of Shieldia spp. (the number of viable bacteria is 10×10 9cfu / mL) 10 copies, Mucor culture solution (viable count 10×10 9 cfu / mL), 10 parts of nano-silica and 35 parts of organic fermentation broth were prepared for later use.

[0034] Nano-silica is mixed with water to obtain a nano-silica suspension, and the pH of the suspension is adjusted to 3.7 so that the surface of the nano-silica is positively charged; a bacterial liquid of YAFEF037 of the Conchoderma spp. and a bacterial liquid of Mucor are centrifuged to remove the supernatant, and the liquids are resuspended with deionized water and then mixed to obtain a bacterial suspension, and the pH of the bacterial suspension is adjusted to 9.8 so that the surface of the bacteria is negatively charged; the bacterial suspension is mixed with the nano-silica suspension, and adsorbed for 1 to 2 hours to obtain a nano-silica fixed bacterial agent; the nano-silica fixed bacterial agent is mixed with an organic fermentation liquid to obtain a mixture; water is added to the mixture to granulate, and the mixture is dried to obtain a nano-bacterial fertilizer.

[0035] Comparative Example 3 The difference from Example 1 is that the bacterial solution of Cercospora spp. YAFEF037 was not used. The specific preparation is as follows: Take 30 parts of rice straw, 30 parts of poultry and livestock manure, 20 parts of bone meal, 5 parts of wood ash, 500 parts of water and 10 parts of EM bacteria agent, ferment them at 28°C for 5 days to obtain organic fermentation liquid.

[0036] Take the multi-main coryneform fungus solution (viable bacteria count is 10×10 9 cfu / mL) 10 copies, Mucor culture solution (viable count 10×10 9 cfu / mL), 10 parts of nano-silica and 35 parts of organic fermentation broth were prepared for later use.

[0037] Nano-silica is mixed with water to obtain a nano-silica suspension, and the pH of the suspension is adjusted to 3.7 so that the surface of the nano-silica is positively charged; a multi-homocysteine fungus liquid and a Mucor fungus fungus liquid are centrifuged to remove the supernatant, and the liquids are resuspended with deionized water and then mixed to obtain a bacterial suspension, and the pH of the bacterial suspension is adjusted to 9.8 so that the surface of the bacteria is negatively charged; the bacterial suspension is mixed with the nano-silica suspension, and adsorbed for 1 to 2 hours to obtain a nano-silica fixed bacterial agent; the nano-silica fixed bacterial agent is mixed with an organic fermentation liquid to obtain a mixture; water is added to the mixture to granulate, and the mixture is dried to obtain a nano-bacterial fertilizer.

[0038] Comparative Example 4 The difference from Example 1 is that no Mucor bacterial solution was used, and the details are as follows: Take 30 parts of rice straw, 30 parts of poultry and livestock manure, 20 parts of bone meal, 5 parts of wood ash, 500 parts of water and 10 parts of EM bacteria agent, ferment them at 28°C for 5 days to obtain organic fermentation liquid.

[0039] Take the multi-main coryneform fungus solution (viable bacteria count is 10×10 9 cfu / mL) 10 copies, YAFEF037 bacterial solution of Conchoderma lucidum (viable bacteria count 10×10 9 cfu / mL), 10 parts of nano-silica and 35 parts of organic fermentation broth were prepared for later use.

[0040] Nano-silica is mixed with water to obtain a nano-silica suspension, and the pH of the suspension is adjusted to 3.7 so that the surface of the nano-silica is positively charged; a fungal solution of Coryneformis multilocus and a fungal solution of YAFEF037 are centrifuged to remove the supernatant, and the solutions are resuspended with deionized water and then mixed to obtain a bacterial suspension, and the pH of the bacterial suspension is adjusted to 9.8 so that the surface of the bacteria is negatively charged; the bacterial suspension is mixed with the nano-silica suspension, and adsorbed for 1 to 2 hours to obtain a nano-silica fixed bacterial agent; the nano-silica fixed bacterial agent is mixed with an organic fermentation liquid to obtain a mixture; water is added to the mixture to granulate the mixture, and the mixture is dried to obtain a nano-bacterial fertilizer.

[0041] Test Example 1 27 plots were randomly selected from the rice experimental field and divided into 9 groups, with 3 replicates in each group. Each group was treated as follows: Group 1: Before planting, the nano-bacterial fertilizer of Example 1 was applied as a base fertilizer at a rate of 10 kg / mu; when the rice was in the bark stage, a topdressing was applied once at a rate of 8 kg / mu; when the rice was in the booting stage, a second topdressing was applied at a rate of 5 kg / mu; Group 2: Before planting, the nano-bacterial fertilizer of Example 2 was applied as a base fertilizer at a rate of 10 kg / mu; when the rice was in the bark stage, a topdressing was applied once at a rate of 8 kg / mu; when the rice was in the booting stage, a second topdressing was applied at a rate of 5 kg / mu; Group 3: Before planting, the nano-bacterial fertilizer of Example 3 was applied as a base fertilizer at a rate of 10 kg / mu; when the rice was in the bark stage, a topdressing was applied once at a rate of 8 kg / mu; when the rice was in the booting stage, a second topdressing was applied at a rate of 5 kg / mu; Group 4: Before planting, the nano-bacterial fertilizer of Comparative Example 1 was applied as base fertilizer at a rate of 10 kg / mu; when the rice was in the barberry stage, topdressing was performed once at a rate of 8 kg / mu; when the rice was in the booting stage, topdressing was performed for the second time at a rate of 5 kg / mu; Group 5: Before planting, the nano-bacterial fertilizer of Comparative Example 2 was applied as base fertilizer at a rate of 10 kg / mu; when the rice was in the barberry stage, topdressing was performed once at a rate of 8 kg / mu; when the rice was in the booting stage, topdressing was performed for the second time at a rate of 5 kg / mu; Group 6: Before planting, the nano-bacterial fertilizer of Comparative Example 3 was applied as base fertilizer at a rate of 10 kg / mu; when the rice was in the barberry stage, topdressing was performed once at a rate of 8 kg / mu; when the rice was in the booting stage, topdressing was performed for the second time at a rate of 5 kg / mu; Group 7: Before planting, the nano-bacterial fertilizer of Comparative Example 4 was applied as a base fertilizer at a rate of 10 kg / mu; when the rice was in the barberry stage, a topdressing was applied once at a rate of 8 kg / mu; when the rice was in the booting stage, a second topdressing was applied at a rate of 5 kg / mu; Group 8: Before planting, commercial nitrogen, phosphorus and potassium fertilizers were applied as base fertilizer at a rate of 10 kg / mu. When the rice was in the barberry stage, topdressing was performed once at a rate of 8 kg / mu. When the rice was in the heading stage, topdressing was performed a second time at a rate of 5 kg / mu. Group 9: Before planting, apply commercially available nitrogen, phosphorus and potassium fertilizers as base fertilizer at a rate of 10 kg / mu; when the rice is in the barberry stage, apply topdressing once at a rate of 8 kg / mu; when the rice is in the heading stage, apply topdressing for the second time at a rate of 5 kg / mu; use blast-resistant pesticide during the barberry stage of rice, use 100 mL of 40% blast-resistant pesticide emulsifiable concentrate per mu, and add 50 kg of water for spraying.

[0042] If at least one leaf of a rice plant had a lesion, the plant was counted as a diseased plant; if at least one panicle of a rice plant had a lesion, the plant was also counted as a diseased plant.

[0043] The number of diseased plants and healthy plants was recorded separately, and the incidence of rice blast in each group was counted. The results are shown in Table 1.

[0044] Incidence rate = diseased plants / total number of surveyed plants × 100% Table 1 Rice blast incidence

[0045] As shown in Table 1, the nano-fertilizer provided by the present invention can effectively reduce the incidence of rice blast, and its prevention effect can reach the level of the chemical agent rice blast. Combining the incidence rates of Group 4 with those of Groups 1 and 8, it can be seen that the use of nano-silicon dioxide to prepare the nano-fertilizer is more conducive to improving the activity of the strain, thereby reducing the incidence of rice blast. Comparing the incidence rates of Groups 5 to 7 with those of Group 1, it can be seen that the simultaneous use of Corynespora multilocus, Cercospora spp. YAFEF037, and Mucor sp. in the present invention can produce a synergistic effect.

[0046] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A rice nanofertilizer for reducing the incidence of rice blast, characterized in that: The nano bacterial fertilizer comprises a multi-main coryneform bacteria liquid, a Shield mold YAFEF037 bacteria liquid, a Mucor bacteria liquid, nano silicon dioxide and an organic matter fermentation liquid.

2. The nano-fertilizer according to claim 1, wherein In parts by mass, the nano-bacterial fertilizer includes 5-15 parts of multi-main Corynespora fungus liquid, 5-15 parts of Conchoderma YAFEF037 fungus liquid, 5-15 parts of Mucor fungus liquid, 20-30 parts of nano-silicon dioxide and 30-50 parts of organic fermentation liquid.

3. The nano-fertilizer according to claim 1, wherein The number of viable bacteria in the multi-main coryneform fungus liquid is ≥10×10 8 cfu / mL; the number of viable bacteria in the YAFEF037 bacterial solution of the Shieldia spp. is ≥10×10 8 cfu / mL; the number of viable bacteria in the Mucor culture solution is ≥10×10 8 cfu / mL.

4. The nano-bacterial fertilizer according to claim 1, characterized in that The organic fermentation liquid is obtained by fermenting 30-40 parts of rice straw, 20-30 parts of poultry and livestock manure, 10-20 parts of bone meal, 3-5 parts of wood ash, 350-700 parts of water and 10-20 parts of EM bacterial agent, based on parts by mass.

5. The nano-bacterial fertilizer according to claim 4, characterized in that The fermentation condition is 28° C. to 30° C. for 3 to 5 days.

6. The method for preparing the nano-bacterial fertilizer according to claim 1, wherein The method comprises the following steps: Nano-silica is mixed with water to obtain a nano-silica suspension, and the pH of the suspension is adjusted to 3.0-4.0 so that the surface of the nano-silica is positively charged; a multi-homocysteine fungus solution, a clematis fungus solution YAFEF037, and a mucor fungus fungus solution are centrifuged to remove the supernatant, resuspended with deionized water, and then mixed to obtain a bacterial suspension, and the pH of the bacterial suspension is adjusted to 9.0-10.0 so that the surface of the bacterial cells is negatively charged; the bacterial suspension is mixed with the nano-silica suspension and adsorbed for 1-2 hours to obtain a nano-silica immobilized bacterial agent; mixing the nano-silica immobilized bacterial agent with the organic matter fermentation liquid to obtain a mixture; The mixture is granulated by adding water and dried to obtain nano-bacterial fertilizer.

7. The preparation method according to claim 6, characterized in that The water content of the nano-bacterial fertilizer is 10% to 20%.

8. The preparation method according to claim 6, characterized in that The drying temperature is 45°C to 55°C.

9. A method for reducing the incidence of rice blast based on the nano-bacterial fertilizer according to any one of claims 1 to 5 or the nano-bacterial fertilizer prepared by the method according to any one of claims 6 to 8, characterized in that: The method comprises: applying fertilizer for the first time before rice sowing, applying fertilizer for the second time during the rice bark forming period, and applying fertilizer for the third time during the rice booting period; the fertilizers applied for the first, second and third fertilizations are all the nano-bacterial fertilizers according to any one of claims 1 to 5.

10. The method according to claim 9, characterized in that The amount of the first fertilization is 10-15 kg / mu; the amount of the second fertilization is 5-10 kg / mu; and the amount of the third fertilization is 5-8 kg / mu.

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