Ultrasonic extraction-based phalaenopsis component extraction detection device and analysis method

Abstract

The invention discloses a phalaenopsis component extraction detection device based on ultrasonic extraction and an analysis method, and relates to the technical field of extraction detection, the phalaenopsis component extraction detection device comprises a workbench, a switching assembly, an extraction assembly and a pipetting assembly, one side of the upper surface of the workbench is provided with a liquid mixing cup, and the other side of the upper surface of the workbench is provided with a sampling cup. By arranging the extraction assembly, a low-speed stirring function is added while a mixed solution is subjected to ultrasonic extraction, and meanwhile, an ultrasonic probe continuously ascends and descends below the liquid level, so that the ultrasonic action range is effectively expanded; by arranging the pipetting assembly, the sampling process is synchronously completed while the extracting solution is subjected to filter pressing, and the sampling process can be independently repeated for multiple times.

Description

Technical Field

[0001] The present invention relates to the technical field of extraction and detection, and in particular to a Phalaenopsis component extraction and detection device and an analysis method based on ultrasonic extraction. Background Art

[0002] Flavonoids found in Phalaenopsis orchid extracts possess anti-inflammatory, anti-tumor, and cardiovascular protective properties. However, prolonged heating during conventional extraction can easily lead to degradation, resulting in reduced yields. Therefore, ultrasonic extraction is necessary. Ultrasonic extraction utilizes the cavitation, mechanical vibration, and thermal effects of ultrasound to accelerate solvent penetration and cell wall disruption, thereby improving the extraction efficiency of target ingredients. Compared to traditional extraction methods, ultrasonic extraction offers advantages such as shorter extraction times, higher efficiency, reduced solvent usage, and low-temperature operation.

[0003] After searching, the Chinese patent with announcement number CN118914426B includes a bracket, a liquid inlet pipe, a mixing barrel, a lead-out pipe, a separation barrel, a liquid outlet pipe, a processing table, a mixing mechanism and a separation mechanism; the bottom end of the bracket is the processing table, the side wall of the mixing barrel is installed with a liquid inlet pipe, and the side wall of the mixing barrel in the opposite direction of the liquid inlet pipe is installed with a lead-out pipe; the separation barrel is installed on the bracket, the separation barrel and the mixing barrel are connected through the lead-out pipe, and the liquid outlet pipe is installed at the lower end of the separation barrel; the mixing mechanism is installed in the mixing barrel; the separation mechanism is installed in the separation barrel; the present invention drives the rotation of the stirring blade of the mixing mechanism by the motor to stir and mix the drug sample put into the feeding port, and the design of the one-way plate ensures that ethanol can overflow evenly and be fully mixed with the drug sample to be tested, thereby improving the extraction efficiency of the target component, and combined with the slope set at the connection between the stirring chamber and the transition chamber, the uniformity of mixing is ensured.

[0004] However, in the detection process of the above scheme, a magnetic adsorbent is added to the filtered extract through a guide plate to complete the detection, and the method of taking part of the extract, that is, full sample detection, is not adopted. This detection method not only cannot recover the sample after detection, making it difficult to repeat the test or other analysis, but also has the problem of difficulty in dynamically adjusting the detection parameters according to the test results; on the other hand, the stirring step of the above scheme is separated from the ultrasonic extraction process, making it only applicable to solutions with low solid-liquid ratio and small volume extraction. Otherwise, there will be problems of dry powder agglomeration and deposition, making it difficult for ultrasound to act evenly. Summary of the Invention

[0005] The purpose of the present invention is to provide a Phalaenopsis component extraction and detection device and analysis method based on ultrasonic extraction, which has the advantages of stirring extraction and sampling detection, and solves the problems raised in the background technology.

[0006] To achieve the above objectives, the present invention provides the following technical solutions: a Phalaenopsis component extraction and detection device based on ultrasonic extraction, comprising a workbench, a switching component, an extraction component, and a pipetting component, wherein a mixing cup is provided on one side of the upper surface of the workbench, and a sampling cup is provided on the other side of the upper surface of the workbench; The switching assembly includes a fixed cylinder fixedly connected to the center of the upper surface of the workbench, which is used to control the working state of the device; The extraction component includes a shift plate 1 fixedly connected to the switching component and arranged directly above the mixing cup, which realizes the ultrasonic extraction process of the Phalaenopsis components; The pipetting assembly includes a second shift plate fixedly connected to the switching assembly and arranged just above the sampling cup, which realizes the filter pressing and sampling process of the extract.

[0007] Preferably, the top end of the fixed cylinder is fixedly connected to a top plate, the inner contour of the bottom end of the fixed cylinder is fixedly connected to a motor 1, the top of the motor 1 is provided with a reciprocating screw rod 1 as the output shaft of the motor 1, a screw rod sleeve 1 is screwed on the outer contour of the upper half of the reciprocating screw rod 1, a fixing rod is fixedly connected to the side circumferential wall of the screw rod sleeve 1, a positioning ring is sleeved on the outer contour of the fixed cylinder, the end of the fixing rod away from the screw rod sleeve 1 is fixedly connected to the inner contour of the positioning ring, and the two ends of the side circumferential wall of the positioning ring are fixedly connected to a connecting rod 1 and a connecting rod 2 respectively.

[0008] Preferably, a guide groove is provided on the outer contour of the upper half of the fixed cylinder, and the guide groove is composed of two vertical grooves and a horizontal groove along the surface of the fixed cylinder. The fixed rod passes through and is limitedly slidably connected with the guide groove.

[0009] Preferably, the shift plate 1 is fixedly connected to the end of the connecting rod 1 away from the positioning ring, the top of the shift plate 1 is fixedly connected to the motor 2, the bottom end of the motor 2 is provided with a reciprocating screw rod 2 as the output shaft of the motor 2, the reciprocating screw rod 2 is rotatably connected to the center of the shift plate 1, and a plurality of positioning rods are fixedly connected to a position near the center of the lower surface of the shift plate, and the plurality of positioning rods are distributed at equal intervals around the reciprocating screw rod 2, a screw rod sleeve 2 is screwed on the outer contour of the reciprocating screw rod 2, the screw rod sleeve 2 is slidably connected by the positioning rod, and an ultrasonic probe is provided on the outer contour of the bottom end of the screw rod sleeve 2.

[0010] Preferably, the bottom end of the reciprocating screw rod 2 is fixedly connected to a base, and a plurality of paddle plates distributed around the base at equal intervals are fixedly connected to the side wall of the base. The plurality of paddle plates are all inclined and their effective radius is consistent with the inner diameter of the mixing cup, and the bottom surfaces of the plurality of paddle plates are horizontally fitted with the bottom inner wall of the mixing cup.

[0011] Preferably, the second shift plate is fixedly connected to the end of the second connecting rod away from the positioning ring, the bottom end of the second shift plate is fixedly connected to a fixed shaft, a fixed column is sleeved through the outer contour of the fixed shaft, a liquid storage groove is provided inside the lower half of the fixed column, a cross groove connected to the liquid storage groove is provided inside the bottom end of the fixed column, the bottom end of the fixed shaft is fixedly connected to a positioning plate slidably connected to the liquid storage groove, and a tension spring sleeved on the outer contour of the fixed shaft is fixedly connected between the upper surface of the positioning plate and the inner wall of the liquid storage groove; The pipetting assembly also includes a liquid storage cylinder fixedly connected to the top of the fixed shaft and penetrated by the second shift plate. The size of the liquid storage cylinder is consistent with the inner diameter of the mixing cup. The bottom end of the liquid storage cylinder is fixedly connected to a bottom plate, which is penetrated and fixedly connected by a fixed column. A plurality of filter holes are opened at equal intervals on the surface of the bottom plate, and a filter screen is provided inside the bottom plate.

[0012] Preferably, valves for controlling the flow direction of the extracting liquid are provided at the intersections of the cross grooves, and the flow direction control of the extracting liquid by the cross grooves is fixed to horizontal end suction and vertical end discharge.

[0013] Preferably, an analysis method comprises the following steps: S1. Preparation of solvent: Weigh 5.00 g of Phalaenopsis orchid powder after sieving through a 60-mesh sieve and place it in a mixing cup. Then, add mL of 70% ethanol solution to the mixing cup to ensure that the ratio of solvent volume to sample mass is 20:1. S2, ultrasonic extraction: Start the switching component to drive the extraction component to insert into the solvent, and then the extraction component starts to work to ultrasonically extract the solvent. During the process, the extraction components work synchronously, dynamically adjust the ultrasonic point and stir the solvent at a low speed; S3. Liquid sampling: After the extraction is completed, the switching component drives the extraction component to be pulled out from the liquid surface, and the liquid transfer component is moved to the top of the mixing cup. The extracted liquid is left to stand for 10 minutes. Then the switching component controls the liquid transfer component to be pressed down. The liquid transfer component completes the pressure filtration process of the extracted liquid. During the process, the extracted liquid is sampled simultaneously. After the sampling is completed, the liquid transfer component is reset. Then the extraction component drives the liquid transfer component to be pressed down again. The liquid transfer component discharges the sample into the sampling cup. S4. Detection and analysis: The sample in the sampling cup is filtered to a fixed volume, and then sent to the HPLC system for chromatographic detection. The content of flavonoid compounds in the Phalaenopsis dry powder is analyzed based on the test results.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention provides an extraction component to perform ultrasonic extraction on the mixed liquid while adding a low-speed stirring function, further preventing the dry powder from agglomerating and sinking to the bottom. At the same time, the ultrasonic probe is continuously raised and lowered below the liquid surface, effectively expanding the ultrasonic action range, thereby improving the ultrasonic extraction effect.

[0015] 2. The present invention provides a pipetting component to filter the extract and simultaneously complete the sampling process, and the sampling process can be repeated multiple times. Through multiple sampling and testing, the accuracy of the test results is effectively guaranteed, while avoiding sample waste and pollution.

[0016] 3. The present invention sets a switching component to enable the device to automatically operate according to the set parameters, greatly saving the steps of manual operation and effectively improving the degree of automation of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the main structure of the present invention; Figure 2 This is a cross-sectional view of the main structure of the present invention; Figure 3 A schematic diagram of a switching component of the present invention; Figure 4 This is an exploded view of the switching assembly of the present invention; Figure 5 This is a schematic diagram of the connection relationship of the present invention; Figure 6 Schematic diagram of the extraction component of the present invention; Figure 7 Schematic diagram of the pipetting assembly of the present invention; Figure 8 This is an example diagram of HPLC chromatographic analysis of the present invention; Figure 9 This is an example diagram of the quercetin concentration curve of the present invention.

[0018] In the figure: 1. Workbench; 11. Mixing cup; 12. Sampling cup; 2. Fixed cylinder; 21. Guide groove; 22. Motor 1; 23. Reciprocating screw 1; 24. Screw sleeve 1; 25. Fixed rod; 26. Positioning ring; 27. Connecting rod 1; 28. Connecting rod 2; 29. Top plate; 3. Shift plate 1; 31. Motor 2; 32. Reciprocating screw 2; 33. Fixed rod; 34. Screw sleeve 2; 35. Ultrasonic probe; 36. Base; 37. Paddle; 4. Shift plate 2; 41. Fixed shaft; 42. Fixed column; 43. Liquid storage tank; 44. Cross slot; 45. Positioning plate; 46. Tension spring; 5. Liquid storage cylinder; 51. Bottom plate; 52. Filter hole; 53. Filter screen. DETAILED DESCRIPTION

[0019] The following will provide a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0020] Example 1:

[0021] See also Figures 1 to 7 The present invention provides a technical solution: a Phalaenopsis component extraction and detection device based on ultrasonic extraction, comprising a workbench 1, a switching component, an extraction component, and a pipetting component, wherein a mixing cup 11 is provided on one side of the upper surface of the workbench 1, and a sampling cup 12 is provided on the other side of the upper surface of the workbench 1; The switching assembly includes a fixed cylinder 2 fixedly connected to the center of the upper surface of the workbench 1, which is used to control the working state of the device; The extraction component includes a shift plate 3 fixedly connected to the switching component and arranged directly above the mixing cup 11, which realizes the ultrasonic extraction process of the Phalaenopsis components; The pipetting assembly includes a shift plate 2 4 fixedly connected to the switching assembly and arranged directly above the sampling cup 12, which realizes the filter pressing and sampling process of the extract.

[0022] In this solution, the Phalaenopsis dry powder and the ethanol solution are mixed by the mixing cup 11, and the extraction component further performs ultrasonic extraction on the mixed solution. During the process, the ultrasonic point position is dynamically adjusted, and the mixed extraction process is further accelerated by low-speed stirring, thereby improving the extraction quality. The extracted liquid is then filtered and sampled using a pipetting assembly. The coarse particles in the extract are further removed by filtration, and the sample is further discharged into the sampling cup 12. Multiple sampling tests are performed to effectively ensure the accuracy of the test results and effectively avoid sample waste.

[0023] Example 2:

[0024] See also Figures 3 to 5 This embodiment is further explained on the basis of the first embodiment: the top end of the fixed cylinder 2 is fixedly connected to a top plate 29, the inner contour of the bottom end of the fixed cylinder 2 is fixedly connected to a motor 22, the top end of the motor 22 is provided with a reciprocating screw 23 as the output shaft of the motor 22, a screw sleeve 24 is screwed on the outer contour of the upper half of the reciprocating screw 23, a fixing rod 25 is fixedly connected to the side wall of the screw sleeve 24, a positioning ring 26 is sleeved on the outer contour of the fixed cylinder 2, the end of the fixing rod 25 away from the screw sleeve 24 is fixedly connected to the inner contour of the positioning ring 26, and the two ends of the side wall of the positioning ring 26 are respectively fixedly connected to a connecting rod 27 and a connecting rod 28.

[0025] A guide groove 21 is provided on the outer contour of the upper half of the fixed cylinder 2 . The guide groove 21 is composed of two vertical grooves and a horizontal groove along the surface of the fixed cylinder 2 . The fixed rod 25 passes through and is connected to the guide groove 21 in a limited sliding manner.

[0026] As can be seen from the first embodiment, the switching component controls the working state of the device by shifting the extraction component and the pipetting component. Figure 1 As shown, the extraction component is located directly above the mixing cup 11, and the pipetting component is located directly above the sampling cup 12. Figure 4 As shown, the guide groove 21 is composed of vertical groove A-horizontal groove-vertical groove B in sequence, with vertical groove A on the right and vertical groove B on the left. At this time, the fixing rod 25 is located at the intersection of the rightmost end of the horizontal groove of the guide groove 21 and the vertical groove A.

[0027] When the device starts working, the motor 22 is started. At this time, the motor 22 drives the reciprocating screw 23 to rotate. The reciprocating screw 23 further has a tendency to drive the screw sleeve 24 and the fixed rod 25 to rotate. However, since the fixed rod 25 is already located at the intersection of the rightmost end of the horizontal groove of the guide groove 21 and the vertical groove B, that is, the fixed rod 25 cannot rotate counterclockwise, which causes the screw sleeve 24 to also be unable to rotate counterclockwise. At this time, the rotation of the reciprocating screw 23, under the action of the screw connection between it and the screw sleeve 24, will drive the screw sleeve 24 to rotate counterclockwise. 24 and the fixed rod 25 slowly descend along the reciprocating screw rod 23. During this process, the fixed rod 25 slides along the vertical groove A of the guide groove 21. Since the positioning ring 26 is fixedly connected to the fixed rod 25, the extraction component and the positioning ring 26 are fixedly connected through the connecting rod 27. That is, the entire extraction component slowly descends synchronously with the rotation of the reciprocating screw rod 23 and is gradually inserted into the mixture of Phalaenopsis dry powder and ethanol solvent in the mixing cup 11. At this time, the motor 22 is stopped, and the extraction component is started, and the extraction component starts the ultrasonic extraction process.

[0028] Furthermore, when the ultrasonic extraction process is completed, the motor 22 is started again to drive the reciprocating screw 23 to rotate. Due to the screw connection effect between the reciprocating screw 23 and the screw sleeve 24, the screw sleeve 24 will automatically reverse after moving to the extreme position. Therefore, the screw sleeve 24 and the extraction component will rise synchronously along the reciprocating screw 23, and the extraction component will gradually be pulled out of the extraction liquid and reset. When the fixed rod 25 is reset to the intersection of the rightmost end of the horizontal groove of the guide groove 21 and the vertical groove A, the reciprocating screw 23 continues to rotate. Since this point will only hinder the counterclockwise rotation of the fixed rod 25, it will not affect the clockwise rotation of the fixed rod 25. That is, at this time, the reciprocating screw 23 will Drive the screw sleeve 24 and the extraction component to rotate clockwise as a whole. During this process, the fixed rod 25 slides clockwise along the transverse groove of the guide groove 21. Similarly, the pipetting component also rotates clockwise along with the screw sleeve 24 due to the fixed connection of the connecting rod 28, until the fixed rod 25 slides through the entire transverse groove of the guide groove 21 and comes to the intersection of the leftmost end of the transverse groove in the guide groove 21 and the vertical groove B. At this time, this point will limit the clockwise rotation of the fixed rod 25, thereby stopping the rotation of the screw sleeve 24, the extraction component and the pipetting component. Subsequently, the rotation of the reciprocating screw 23 will cause the screw sleeve 24, the extraction component and the pipetting component to descend as a whole due to the screw connection between it and the screw sleeve 24.

[0029] Since the arc center angle of the horizontal groove of the guide groove 21 is 180 degrees, that is, the angle of the extraction component and the pipetting component when rotating with the screw sleeve 24 is also 180 degrees, the extraction component and the pipetting component are swapped. At this time, the pipetting component is located directly above the mixing cup 11. Then, as the pipetting component descends, the extraction liquid in the mixing cup 11 is filtered and sampled.

[0030] After the sampling operation is completed, the motor 22 is driven again to start the reciprocating screw 23 to rotate, completing the reset of the entire device. After the reset, the pipetting assembly is again located directly above the sampling cup 12, and then the reciprocating screw 23 continues to rotate to control the pipetting assembly to move downward, and the pipetting assembly contacts the sampling cup 12 and discharges the sample into the sampling cup 12.

[0031] Example 3:

[0032] See also Figure 5 and Figure 6, this embodiment is further explained on the basis of the second embodiment: the shift plate 1 3 is fixedly connected to the end of the connecting rod 1 27 away from the positioning ring 26, the top of the shift plate 1 3 is fixedly connected to the motor 2 31, and the bottom end of the motor 2 31 is provided with a reciprocating screw 2 32 as the output shaft of the motor 2 31, the reciprocating screw 2 32 is rotatably connected to the center of the shift plate 1 3, and a plurality of positioning rods 33 are fixedly connected to the position near the center of the lower surface of the shift plate 1 3, and the plurality of positioning rods 33 are distributed at equal intervals around the reciprocating screw 2 32, and a screw sleeve 2 34 is screwed on the outer contour of the reciprocating screw 2 32, and the screw sleeve 2 34 is slidably connected by the positioning rod 33, and an ultrasonic probe 35 is provided on the outer contour of the bottom end of the screw sleeve 2 34.

[0033] The bottom end of the reciprocating screw rod 32 is fixedly connected to a base 36, and a plurality of paddle plates 37 distributed around the side wall of the base 36 are fixedly connected. The plurality of paddle plates 37 are all inclined and their effective radius is consistent with the inner diameter of the mixing cup 11. The bottom surfaces of the plurality of paddle plates 37 are all horizontally attached to the bottom inner wall of the mixing cup 11.

[0034] As can be seen from the second embodiment, the ultrasonic extraction process is completed after the extraction component is inserted into the mixed liquid in the mixing cup 11. When the extraction component is fully inserted into the mixing cup 11 and the bottom end of the paddle 37 contacts the inner wall of the bottom end of the mixing cup 11, the motor 1 22 is stopped and the motor 2 31 is turned on. At this time, the motor 2 31 drives the reciprocating screw 2 32 to rotate synchronously. The reciprocating screw 2 32 has a tendency to drive the positioning rod 33 to rotate under the screw connection between it and the screw sleeve 2 34. However, since the shift plate 1 3 is fixedly connected to the connecting rod 1 27, the positioning rod 33 is also fixedly connected. The cam 32 is connected to the lower surface of the shift plate 3, that is, the shift plate 3 and the positioning rod 33 are in a fixed state. Since the positioning rod 33 passes through the screw sleeve 2 34, the screw sleeve 2 34 can only slide along the positioning rod 33, and cannot rotate with the reciprocating screw rod 2 32. At this time, the rotation of the reciprocating screw rod 2 32 will drive the screw sleeve 2 34 to slide vertically along the positioning rod 33, and after sliding to the limit position, under the screw connection effect of the reciprocating screw rod 2 32 and the screw sleeve 2 34, the screw sleeve 2 34 will automatically reverse, thereby realizing reciprocating lifting motion.

[0035] At the same time, the reciprocating screw 32 always drives the base 36 and the paddle 37 to rotate synchronously. The paddle 37 stirs the mixed liquid in the mixing cup 11 at a low speed. At the same time, its inclined setting disturbs the dry powder particles deposited at the bottom of the solvent, further increasing the diffusion movement of the dry powder particles in the solvent, thereby improving the ultrasonic effect to enhance the extraction effect. At the same time, the design of the bottom surface of the paddle 37 fitting the inner wall of the bottom end of the mixing cup 11 can effectively scrape the agglomerated dry powder adhering to the inner wall of the mixing cup 11, further ensuring the mixing quality of the dry powder.

[0036] On the other hand, the ultrasonic probe 35 performs ultrasonic extraction on the mixed liquid, and the specific ultrasonic parameters are: frequency 40kHz, power 250W, temperature range of 25±2℃, and ultrasonic time of 30min; at the same time, the ultrasonic probe 35 performs slow reciprocating lifting and lowering motion synchronously with the screw sleeve 34, that is, the ultrasonic action point of the ultrasonic probe 35 on the mixed liquid is in a real-time changing process, thereby expanding the ultrasonic effect of the ultrasonic probe 35 on the mixed liquid through the continuous movement of the ultrasonic point, and further improving the extraction quality.

[0037] It should be noted that since the reciprocating screw rod 32 is completely immersed in the mixed liquid, that is, the lifting movement of the ultrasonic probe 35 is always below the liquid surface, it is effectively prevented that the ultrasonic probe 35 exceeds the liquid surface during the lifting process, causing bubbles to be mixed into the solvent.

[0038] Example 4:

[0039] See also Figure 5 and Figure 7 , this embodiment is further explained on the basis of the third embodiment: the shift plate 2 4 is fixedly connected to the end of the connecting rod 28 away from the positioning ring 26, the bottom end of the shift plate 2 4 is fixedly connected to a fixed shaft 41, the outer contour of the fixed shaft 41 is penetrated by a fixed column 42, the interior of the lower half of the fixed column 42 is provided with a liquid storage groove 43, the interior of the bottom end of the fixed column 42 is provided with a cross groove 44 connected to the liquid storage groove 43, the bottom end of the fixed shaft 41 is fixedly connected to a positioning plate 45 slidably connected to the liquid storage groove 43, and a tension spring 46 sleeved on the outer contour of the fixed shaft 41 is fixedly connected between the upper surface of the positioning plate 45 and the inner wall of the liquid storage groove 43; The pipetting assembly also includes a liquid storage cylinder 5 fixedly connected to the top of the fixed shaft 41 and penetrated by the shift plate 2 4. The size of the liquid storage cylinder 5 is consistent with the inner diameter of the mixing cup 11. The bottom end of the liquid storage cylinder 5 is fixedly connected to a bottom plate 51, and the bottom plate 51 is penetrated and fixedly connected by the fixed column 42. A plurality of filter holes 52 are evenly spaced around the surface of the bottom plate 51, and a filter screen 53 is provided inside the bottom plate 51.

[0040] The cross slots 44 are provided with valves for controlling the flow direction of the extracting liquid at the intersections thereof. The cross slots 44 control the flow direction of the extracting liquid to be fixedly sucked in at the horizontal end and discharged at the vertical end.

[0041] As can be seen from Example 2, after the ultrasonic extraction is completed, the control device is reset and left to stand for 10 minutes. At this time, the coarse particles of the extract in the mixing cup 11 are completely precipitated. Then, according to the process described in Example 2, the pipetting assembly is controlled to rotate to the top of the mixing cup 11 and inserted into the mixing cup 11.

[0042] Since the size of the liquid storage cylinder 5 fits the inner wall of the mixing cup 11, the liquid storage cylinder 5 will press the extract in the mixing cup 11 after being inserted into the mixing cup 11. As the liquid storage cylinder 5 is pressed downward, the extract enters the interior of the liquid storage cylinder 5 through the filter hole 52. During this process, the filter screen 53 is used to complete the filtration operation of the extract until the bottom end of the bottom plate 51 contacts the bottom inner wall of the mixing cup 11, and the overall filtration operation is completed. It should be noted that the filter hole 52 is set to a unidirectional conduction mode, that is, the extract can only enter the liquid storage cylinder 5 through the filter hole 52, and cannot flow out of the liquid storage cylinder 5 through the filter hole 52.

[0043] When the bottom end of the bottom plate 51 contacts the bottom inner wall of the mixing cup 11, the fixed rod 25 has not yet moved down to the lowest end along the vertical groove B of the guide groove 21. That is, at this time, the shift plate 24 and the fixed shaft 41 still have a tendency to move down with the connecting rod 28 and the screw sleeve 1 24, but the fixed column 42 has been in a fixed state along with the liquid storage cylinder 5. That is, there is relative movement between the fixed shaft 41 and the fixed column 42. The fixed shaft 41 drives the positioning plate 45 to slide in the liquid storage groove 43, so that the air in the liquid storage groove 43 is discharged from the vertical end of the cross groove 44. During this process, the tension spring 46 is synchronously stretched.

[0044] Subsequently, with the completion of the filtration operation, the rotation of the reciprocating screw 23 causes the pipetting assembly to move upward as a whole, and the shift plate 2 4 drives the fixed shaft 41 to move upward. Before the tension spring 46 is stretched and reset, the fixed column 42 continues to remain fixed with the liquid storage cylinder 5, thereby increasing the volume of the liquid storage tank 43, thereby sucking the extracted liquid in the liquid storage cylinder 5 into the liquid storage tank 43 along the horizontal end of the cross groove 44 until the tension spring 46 is reset, completing the sampling operation. At this time, the positioning plate 45 drives the fixed column 42 and the liquid storage cylinder 5 to move upward synchronously, and then controls the pipetting assembly to rotate above the sampling cup 12.

[0045] The pipetting assembly is controlled to descend again, and as the bottom surface of the bottom plate 51 contacts the sampling cup 12, the liquid storage cylinder 5 and the fixed column 42 are restricted again. Then the shift plate 2 4 and the screw sleeve 1 24 drive the fixed shaft 41 and the positioning plate 45 to squeeze the liquid storage groove 43 again, thereby discharging the sample in the liquid storage groove 43 into the sampling cup 12 along the vertical end of the cross groove 44.

[0046] When multiple sampling is required, the pipetting assembly is controlled to rise and fall repeatedly above the sampling cup 12. When rising, the extraction liquid in the liquid storage cylinder 5 is sucked into the liquid storage tank 43. When descending, the sample in the liquid storage tank 43 is discharged into the sampling cup 12 until the sample amount in the sampling cup 12 meets the conditions for multiple sampling.

[0047] Embodiment 5:

[0048] See also Figure 7 and Figure 8 This embodiment further illustrates, based on the fourth embodiment, an analysis method comprising the following steps: S1. Preparation of solvent: Weigh 5.00 g of Phalaenopsis orchid powder sieved through a 60-mesh sieve and place it in a mixing cup 11. Then, add 100 mL of 70% ethanol solution to the mixing cup 11, ensuring that the ratio of solvent volume to sample mass is 20:1. S2, ultrasonic extraction: Start the switching component to drive the extraction component to insert into the solvent, and then the extraction component starts to work to ultrasonically extract the solvent. During the process, the extraction components work synchronously, dynamically adjust the ultrasonic point and stir the solvent at a low speed; S3. Liquid sampling: After the extraction is completed, the switching component drives the extraction component to be pulled out from the liquid surface, and the liquid transfer component is moved to the top of the mixing cup 11. The extracted liquid is allowed to stand for 10 minutes. Then the switching component controls the liquid transfer component to be pressed down. The liquid transfer component completes the pressure filtration process of the extracted liquid. During the process, the extracted liquid is sampled simultaneously. After the sampling is completed, the liquid transfer component is controlled to be reset. Then the extraction component drives the liquid transfer component to be pressed down again. The liquid transfer component discharges the sample into the sampling cup 12. S4. Detection and analysis: The sample in the sampling cup 12 is filtered to a fixed volume, and then sent to the HPLC system to complete chromatographic detection, and the content of flavonoid compounds in the Phalaenopsis dry powder is analyzed based on the detection results.

[0049] In this method, an HPLC system is used to perform chromatographic analysis on the samples. Phase A in the mobile phase is 0.1% aqueous phosphoric acid, and phase B is acetonitrile solution. The chromatographic column is a C18 column 250 mm × 4.6 mm, 5 μm. The sample in sampling cup 12 is transferred to a volumetric flask and the sample volume is adjusted to 10 mL with HPLC-grade methanol or the initial mobile phase. The adjusted sample is filtered through a 0.22 μm organic filter membrane to remove particulate impurities. The filtrate is collected and placed in an HPLC vial for detection.

[0050] The HPLC system was then started, and the parameters were set as follows: flow rate: 1.0 mL / min, detection wavelength: 360 nm, column temperature: 30°C, injection volume: 10 μL; the gradient elution program was set as follows: 0–10 min: phase B increased from 15% to 30%; 10–30 min: phase B increased from 30% to 60%.

[0051] Based on the above parameter conditions, we can get Figure 8 The chromatogram of Phalaenopsis orchid extract components shown in the figure shows three main peaks at retention times of approximately 10.5 min, 17.8 min, and 24.3 min, respectively. Peak A corresponds to apigenin, peak B corresponds to quercetin, and peak C corresponds to kaempferol.

[0052] Furthermore, through multiple sampling tests, we found that Figure 9The quercetin concentration curve shown in the figure is as follows: × is the coordinate point of the standard series, the straight line is the linear fitting curve drawn based on the coordinate point of the standard series, and the dotted line is the concentration corresponding to the peak area of quercetin in the sample.

[0053] by Figure 9 For example, the fitting equation is: peak area = 10.02 × concentration + 0.19, where the slope 10.02 and intercept 0.19 are calculated based on the standard point data according to linear regression. The device needs to be recalibrated each time it is turned on. Taking the peak area of quercetin peak B as an example, which is 252 mAU·s, then Figure 9 As shown by the middle dotted line, the concentration of quercetin in the sample at this time is about 25.15 μg / mL.

[0054] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A Phalaenopsis component extraction and detection device based on ultrasonic extraction, comprising a workbench (1), characterized in that: It also includes a switching component, an extraction component, and a pipetting component. A mixing cup (11) is provided on one side of the upper surface of the workbench (1), and a sampling cup (12) is provided on the other side of the upper surface of the workbench (1). The switching assembly comprises a fixed cylinder (2) fixedly connected to the center of the upper surface of the workbench (1), and is used to control the working state of the device; The extraction component includes a shift plate (3) fixedly connected to the switching component and arranged directly above the mixing cup (11), which realizes the ultrasonic extraction process of the Phalaenopsis components; The liquid transfer assembly comprises a second shift plate (4) fixedly connected to the switching assembly and arranged directly above the sampling cup (12), which realizes the filter pressing and sampling process of the extract.

2. The ultrasonic extraction and detection device for Phalaenopsis components according to claim 1, characterized in that: The top end of the fixed cylinder (2) is fixedly connected to a top plate (29), the inner contour of the bottom end of the fixed cylinder (2) is fixedly connected to a motor 1 (22), the top end of the motor 1 (22) is provided with a reciprocating screw 1 (23) as an output shaft of the motor 1 (22), the outer contour of the upper half of the reciprocating screw 1 (23) is screwed with a screw sleeve 1 (24), the side wall of the screw sleeve 1 (24) is fixedly connected to a fixed rod (25), the outer contour of the fixed cylinder (2) is sleeved with a positioning ring (26), the end of the fixed rod (25) away from the screw sleeve 1 (24) is fixedly connected to the inner contour of the positioning ring (26), and the two ends of the side wall of the positioning ring (26) are fixedly connected to a connecting rod 1 (27) and a connecting rod 2 (28), respectively.

3. The ultrasonic extraction and detection device for Phalaenopsis components according to claim 2, characterized in that: A guide groove (21) is provided on the outer contour of the upper half of the fixed cylinder (2). The guide groove (21) is composed of two vertical grooves and a horizontal groove along the surface of the fixed cylinder (2). The fixed rod (25) passes through and is connected to the guide groove (21) in a limited sliding manner.

4. The ultrasonic extraction and detection device for Phalaenopsis components according to claim 1, characterized in that: The shift plate 1 (3) is fixedly connected to the end of the connecting rod 1 (27) away from the positioning ring (26), the top of the shift plate 1 (3) is fixedly connected to the motor 2 (31), the bottom end of the motor 2 (31) is provided with a reciprocating screw rod 2 (32) as the output shaft of the motor 2 (31), the reciprocating screw rod 2 (32) is connected to the center of the shift plate 1 (3) through rotation, a plurality of positioning rods (33) are fixedly connected to the position near the center of the lower surface of the shift plate 1 (3), the plurality of positioning rods (33) are distributed at equal intervals around the reciprocating screw rod 2 (32), a screw rod sleeve 2 (34) is screwed on the outer contour of the reciprocating screw rod 2 (32), the screw rod sleeve 2 (34) is slidably connected by the positioning rod (33), and an ultrasonic probe (35) is provided on the outer contour of the bottom end of the screw rod sleeve 2 (34).

5. The ultrasonic extraction and detection device for Phalaenopsis components according to claim 4, characterized in that: The bottom end of the reciprocating screw rod (32) is fixedly connected to a base (36), and a plurality of paddle plates (37) distributed around the side wall of the base (36) are fixedly connected. The plurality of paddle plates (37) are all inclined and their effective radius is consistent with the inner diameter of the mixing cup (11). The bottom surfaces of the plurality of paddle plates (37) are all horizontally attached to the bottom inner wall of the mixing cup (11).

6. The ultrasonic extraction and detection device for Phalaenopsis components according to claim 1, characterized in that: The shift plate 2 (4) is fixedly connected to the end of the connecting rod 2 (28) away from the positioning ring (26), and the bottom end of the shift plate 2 (4) is fixedly connected to a fixed shaft (41), and a fixed column (42) is sleeved through the outer contour of the fixed shaft (41), and a liquid storage groove (43) is provided inside the lower half of the fixed column (42), and a cross groove (44) connected to the liquid storage groove (43) is provided inside the bottom end of the fixed column (42), and the bottom end of the fixed shaft (41) is fixedly connected to a positioning plate (45) slidably connected to the liquid storage groove (43), and a tension spring (46) sleeved on the outer contour of the fixed shaft (41) is fixedly connected between the upper surface of the positioning plate (45) and the inner wall of the liquid storage groove (43); The pipetting assembly further comprises a liquid storage cylinder (5) fixedly connected to the top of the fixed shaft (41) and penetrated by the second shift plate (4), the size of the liquid storage cylinder (5) being consistent with the inner diameter of the mixing cup (11), the bottom end of the liquid storage cylinder (5) being fixedly connected to a bottom plate (51), the bottom plate (51) being penetrated and fixedly connected by the fixed column (42), a plurality of filter holes (52) being uniformly spaced around the surface of the bottom plate (51), and a filter screen (53) being provided inside the bottom plate (51).

7. The ultrasonic extraction and detection device for Phalaenopsis components according to claim 6, characterized in that: A valve for controlling the flow direction of the extracting liquid is provided at the intersection of the cross groove (44), and the cross groove (44) controls the flow direction of the extracting liquid to be fixed as horizontal end suction-vertical end discharge.

8. An analysis method, applied to the ultrasonic extraction and detection device for Phalaenopsis components according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1. Preparation of solvent: Weigh 5.00 g of Phalaenopsis orchid powder after sieving through a 60-mesh sieve and place it in a mixing cup (11). Then, add 100 mL of 70% ethanol solution into the mixing cup (11), ensuring that the ratio of solvent volume to sample mass is 20:

1. S2, ultrasonic extraction: Start the switching component to drive the extraction component to insert into the solvent, and then the extraction component starts to work to ultrasonically extract the solvent. During the process, the extraction components work synchronously, dynamically adjust the ultrasonic point and stir the solvent at a low speed; S3, pipetting sampling: After the extraction is completed, the switching component drives the extraction component to be pulled out from the liquid surface, and the pipetting component is moved to the top of the mixing cup (11). The extraction liquid is left to stand for 10 minutes. Then the switching component controls the pipetting component to press down. The pipetting component completes the filtration process of the extraction liquid. During the process, the extraction liquid is sampled simultaneously. After the sampling is completed, the pipetting component is controlled to reset. Then the extraction component drives the pipetting component to press down again. The pipetting component discharges the sample into the sampling cup (12); S4. Detection and analysis: The sample in the sampling cup (12) is filtered to a fixed volume, and then sent to the HPLC system for chromatographic detection, and the content of flavonoid compounds in the Phalaenopsis dry powder is analyzed based on the detection results.

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