EPS targeted cleaning type medical nano-enzyme cleaning agent and preparation method thereof

Abstract

The invention relates to an EPS targeted cleaning type medical nano-enzyme cleaning agent and a preparation method thereof. The cleaning agent comprises the following components in percentage by weight: 3%-5% of trehalose, 5%-10% of glycerol, 9%-11% of nano compound enzyme, 3%-5% of sodium xylene sulfonate, 0.5%-1.5% of a builder, 0.5%-1.5% of ethylenediamine tetraacetic acid, 0.3%-0.7% of benzotriazole, 0.1%-0.5% of a defoaming agent, 0.1%-0.5% of a preservative, 0.05%-0.15% of triethanolamine and the balance of pure water. Through strict verification, the EPS targeted removal type medical nano-enzyme cleaning agent can quickly remove EPS (extracellular polymer matrix) on medical instruments such as endoscopes and the like in an extremely short time, effectively prevents the formation of biological membranes, has the bacterium removal rate of 95% and the biological membrane removal rate of 90%, and has remarkable effects on reducing the infection rate of hospitals and improving the medical safety.

Description

Technical Field

[0001] The present invention relates to the technical field of medical cleaning agents, and in particular to an EPS targeted removal medical nanoenzyme cleaning agent and a preparation method thereof. Background Art

[0002] As an invasive and minimally invasive medical device, endoscopes play a vital role in clinical practice. Endoscope cleaning and disinfection have become a key link in hospital infection control. However, cross-infection caused by endoscopic examinations is becoming increasingly serious. Among them, infections such as Helicobacter pylori, human immunodeficiency virus, and hepatitis B virus are closely related to the presence of bacterial biofilms in the endoscope lumen. Biofilms are the habitat of bacteria and an important cause of the formation of drug-resistant bacteria. Extracellular polymeric matrix (EPS) is an important component of biofilms. Strengthening the cleaning and disinfection of endoscopes and removing biofilms are key links in preventing hospital infections. Hospital infections are a major hidden danger in the medical process. They not only increase the pain and treatment costs of patients, but may even be life-threatening in severe cases.

[0003] Traditional medical device cleaning agents often contain numerous chemical ingredients, such as phosphates and surfactants. Excessive use of these chemicals can pollute the environment. Furthermore, these traditional chemical cleaning agents are ineffective in removing biofilms and pathogens that adhere to device surfaces, leading to a high incidence of nosocomial infections (according to a survey of 10,000 endoscopy patients in 10 different hospitals, the incidence of nosocomial infections was 2% when using traditional chemical cleaning agents). Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide an EPS targeted removal medical nanoenzyme cleaning agent and a preparation method thereof, so as to solve the defects and shortcomings of the current use of traditional chemical cleaning agents for medical devices such as endoscopes, as described in the background technology.

[0005] The technical solution adopted by the present invention to solve its technical problems is: an EPS targeted removal type medical nanoenzyme cleaning agent, containing the following ingredients in percentage by weight: 3% to 5% trehalose, 5% to 10% glycerol, 9% to 11% nanocomposite enzyme, 3% to 5% sodium xylene sulfonate, 0.5% to 1.5% detergent, 0.5% to 1.5% ethylenediaminetetraacetic acid, 0.3% to 0.7% benzotriazole, 0.1% to 0.5% defoaming agent, 0.1% to 0.5% preservative, 0.05% to 0.15% triethanolamine, and the balance is pure water.

[0006] Furthermore, the nanocomposite enzyme is a mixture of fructase, protease and glucanase, and the weight ratio of the three enzymes is 1:(1.5-2):(1-1.5).

[0007] Preferably, the builder is a mixture of sodium silicate and sodium carboxymethyl cellulose, and the weight ratio of sodium silicate to sodium carboxymethyl cellulose is 1:1.

[0008] Preferably, the defoaming agent is a mixture of one or more of polydimethylsiloxane, polyoxypropylene glycerol ether, octanol, hexadecanol, acetylene alcohol, glycol, tributyl phosphate, sodium octyl phosphate, aluminum stearate, and calcium oleate.

[0009] Preferably, the preservative is benzoisothiazoline-3-one or chloromethylisothiazolinone.

[0010] A method for preparing the EPS targeted removal medical nanoenzyme cleaning agent as described above comprises the following steps:

[0011] a. Pour some pure water into the container, add trehalose, glycerol, sodium xylene sulfonate, and stir until completely dissolved;

[0012] b. Add defoamer and continue stirring to mix evenly;

[0013] c. Add nanocomposite enzyme, detergent, EDTA, benzotriazole, preservative and the remaining pure water, continue stirring to mix evenly, add triethanolamine during stirring to adjust the pH value to 7 to 9;

[0014] d. After aging, the mixture is filtered to obtain the finished product of the EPS targeted removal medical nanoenzyme cleaning agent.

[0015] Furthermore, in the preparation method of the above-mentioned EPS targeted removal medical nanoenzyme cleaning agent, the aging time in step d is 48 hours to 72 hours.

[0016] The beneficial effects of the present invention are: compared with the traditional medical device cleaning agents currently used, the EPS targeted removal medical nanoenzyme cleaning agent of the present invention has been strictly tested and verified to be able to quickly remove EPS (extracellular polymer matrix) on medical devices such as endoscopes in a very short time, effectively prevent the formation of biofilms, and has a bacterial removal rate of up to 95% and a biofilm removal rate of 90%, which has significant effects and significance on reducing hospital infection rates and improving medical safety.

[0017] Compared with traditional medical device cleaning agents, this EPS targeted removal medical nanoenzyme cleaning agent does not contain chemical components that are harmful to the environment, and can therefore greatly reduce the pollution pressure caused by medical waste liquid to water and the environment.

[0018] In terms of usage cost, the use of the EPS targeted removal medical nanoenzyme cleaning agent of the present invention to clean endoscopes makes the average annual maintenance cost of endoscopes 80% lower than that of cleaning with traditional cleaning agents, which undoubtedly provides strong support for hospitals in controlling operating costs; at the same time, the significant improvement in the cleaning efficiency of endoscopes greatly improves the turnover rate of endoscopes, thereby achieving greater economic benefits.

[0019] The EPS targeted removal medical nanoenzyme cleaning agent of the present invention can also be used in other industries with similar cleaning needs, such as food processing, animal husbandry, and water treatment, showing broad market prospects and having positive significance for public health and environmental protection. DETAILED DESCRIPTION

[0020] The present invention is further described in detail below with reference to the following examples and comparative examples. All raw materials used in the examples are commercially available.

[0021] Example 1

[0022] A method for preparing an EPS targeted removal medical nanoenzyme cleaning agent comprises the following steps:

[0023] a. Add 54kg of pure water to a stirred tank, followed by 3kg of trehalose, 5kg of glycerol, and 3kg of sodium xylene sulfonate, and stir for 10 minutes until completely dissolved;

[0024] b. Add 0.1kg polydimethylsiloxane and continue stirring for 10 minutes until mixed;

[0025] c. Add 2.5kg fructase, 4kg protease, 2.5kg glucanase, 0.5kg sodium silicate and sodium carboxymethyl cellulose mixture (containing 0.25kg sodium silicate and 0.25kg sodium carboxymethyl cellulose), 0.5kg ethylenediaminetetraacetic acid, 0.3kg benzotriazole, 0.1kg benzisothiazoline-3-one and 23.45kg pure water, stir for 15 minutes until the mixture is uniform. During the stirring process, add 0.05kg triethanolamine to adjust the pH value to 7-9;

[0026] d. After aging for 48 hours, the product was filtered through 3um and 0.1um secondary filtration to obtain the finished product of EPS targeted removal medical nanoenzyme cleaning agent. The filter residue was rinsed with pure water and collected for the next batch of production.

[0027] Example 2

[0028] A method for preparing an EPS targeted removal medical nanoenzyme cleaning agent comprises the following steps:

[0029] a. Add 47kg of pure water to a stirred tank, followed by 4kg of trehalose, 7.5kg of glycerol, and 4kg of sodium xylene sulfonate, and stir for 10 minutes until completely dissolved;

[0030] b. Add 0.3kg of a mixture of dimethylsiloxane and octanol (containing 0.15kg of dimethylsiloxane and 0.15kg of octanol) and continue stirring for 10 minutes until uniformly mixed;

[0031] c. Add 2.3kg fructase, 4.6kg protease, 3kg glucanase, 1kg sodium silicate and sodium carboxymethyl cellulose mixture (containing 0.25kg sodium silicate and 0.25kg sodium carboxymethyl cellulose), 1kg EDTA, 0.5kg benzotriazole, 0.3kg homobenzisothiazolin-3-one and 21.3kg pure water, and stir for 15 minutes until the mixture is uniform. During the stirring process, add 0.1kg triethanolamine to adjust the pH value to 7-9;

[0032] d. After aging for 36 hours, the product was filtered through 3 μm and 0.1 μm secondary filtration to obtain the finished product of EPS targeted removal medical nanoenzyme cleaning agent. The filter residue was rinsed with pure water and collected for the next batch of production.

[0033] Example 3

[0034] A method for preparing an EPS targeted removal medical nanoenzyme cleaning agent comprises the following steps:

[0035] a. Add 41kg of pure water to a stirred tank, followed by 5kg of trehalose, 10kg of glycerol, and 5kg of sodium xylene sulfonate, and stir for 10 minutes until completely dissolved;

[0036] b. Add 0.3kg polyoxypropylene glycerol ether and continue stirring for 10min until mixed;

[0037] c. Add 2.8 kg fructase, 4.2 kg protease, 4 kg glucanase, 1.5 kg sodium silicate and sodium carboxymethyl cellulose mixture (containing 0.75 kg sodium silicate and 0.75 kg sodium carboxymethyl cellulose), 1.5 kg ethylenediaminetetraacetic acid, 0.7 kg benzotriazole, 0.5 kg chloromethylisothiazolinone and 18.15 kg pure water, stir for 15 minutes until the mixture is uniform, and add 0.15 kg triethanolamine during the stirring process to adjust the pH value to 7-9;

[0038] d. After aging for 72 hours, the product was filtered through 3 μm and 0.1 μm secondary filtration to obtain the finished product of EPS targeted removal medical nanoenzyme cleaning agent. The filter residue was rinsed with pure water and collected for the next batch of production.

[0039] Comparative Example 1

[0040] The difference between Comparative Example 1 and Example 2 is that no trehalose and glycerol are added in step a.

[0041] Comparative Example 2

[0042] The difference between Comparative Example 2 and Example 2 is that no trehalose is added in step a.

[0043] Comparative Example 3

[0044] The difference between Comparative Example 3 and Example 2 is that the nanocomposite enzyme added in step c is a mixture of fructase and protease, wherein the content of fructase is 5.3 kg and the content of protease is 4.6 kg.

[0045] Comparative Example 4

[0046] The difference between Comparative Example 4 and Example 2 is that the nanocomposite enzyme added in step c is a mixture of glucanase and protease, wherein the content of glucanase is 5.3 kg and the content of protease is 4.6 kg.

[0047] Comparative Example 5

[0048] The difference between Comparative Example 5 and Example 2 is that the 1 kg of ethylenediaminetetraacetic acid added in step c of Example 2 is replaced by 1 kg of gluconic acid.

[0049] Testing the cleaning effect: According to Appendix F of the medical cleaning agent industry standard, the cleaning agents prepared in each embodiment and comparative example were subjected to artificial simulated pollutant tests to obtain the pollutant removal rate. The higher the pollutant removal rate, the better the cleaning effect of the cleaning agent.

[0050] Table 1 records the cleaning agent test results.

[0051] Table 1

[0052] Cleaning agent samples prepared in each case Pollutant (including bacteria and biofilm) removal rate Example 1 Bacteria removal rate: 94%; biofilm removal rate: 90% Example 2 Bacteria removal rate: 95%; biofilm removal rate: 92% Example 3 Bacteria removal rate: 93%; biofilm removal rate: 91% Comparative Example 1 Bacteria removal rate: 90%; biofilm removal rate: 80% Comparative Example 2 Bacteria removal rate: 92%; biofilm removal rate: 85% Comparative Example 3 Bacteria removal rate: 93%; biofilm removal rate: 86% Comparative Example 4 Bacteria removal rate: 93%; biofilm removal rate: 86% Comparative Example 5 Bacteria removal rate: 85%; biofilm removal rate: 70%

[0053] As can be seen from the table above, the cleaning agent prepared in Example 2 has the best cleaning effect. Comparative Examples 1 and 2 show that the addition of trehalose and glycerol has a significant impact on the performance of the cleaning agent. The reason is that trehalose and glycerol can maintain the stability of the cleaning agent at 60°C, ensuring the activity of the enzyme in a high temperature environment.

[0054] Comparative Examples 3 and 4 show that, among the nanocomposite enzymes, fructase, protease and glucanase play a very important role in the performance of the cleaning agent, especially in the removal of biofilms, and their combined use can achieve the best effect.

[0055] From Comparative Example 5, it can be seen that the role of EDTA in the cleaning agent is crucial because EDTA (also known as EDTA) is a chelating agent that can chelate calcium (Ca 2+ ), magnesium (Mg 2+ ) and other metal ions, reducing the concentration of free calcium and magnesium ions in the extracellular environment, thereby greatly improving the synergistic clearance rate of other ingredients in the detergent.

[0056] The above embodiments are only used to illustrate the present invention, and are not intended to limit the protection of the present invention. Any non-substantial changes based on the essential solution of the present invention should fall within the scope of protection of the present invention.

Claims

1. A medical nanoenzyme cleaning agent for targeted EPS removal, characterized by: The invention contains the following ingredients in percentage by weight: 3% to 5% of trehalose, 5% to 10% of glycerol, 9% to 11% of nanocomposite enzyme, 3% to 5% of sodium xylene sulfonate, 0.5% to 1.5% of detergent, 0.5% to 1.5% of ethylenediaminetetraacetic acid, 0.3% to 0.7% of benzotriazole, 0.1% to 0.5% of defoaming agent, 0.1% to 0.5% of preservative, 0.05% to 0.15% of triethanolamine, and the balance is pure water.

2. The EPS targeted removal medical nanoenzyme cleaning agent according to claim 1, characterized in that: The nano composite enzyme is prepared by mixing fructase, protease and glucanase, and the weight ratio of the three enzymes is 1:(1.5-2):(1-1.5).

3. The EPS targeted removal medical nanoenzyme cleaning agent according to claim 1, characterized in that: The builder is a mixture of sodium silicate and sodium carboxymethyl cellulose, and the weight ratio of sodium silicate to sodium carboxymethyl cellulose is 1:

1.

4. The EPS targeted removal medical nanoenzyme cleaning agent according to claim 1, characterized in that: The defoaming agent is a mixture of one or more of polydimethylsiloxane, polyoxypropylene glycerol ether, octanol, hexadecanol, acetylene alcohol, glycol, tributyl phosphate, sodium octyl phosphate, aluminum stearate, and calcium oleate.

5. The EPS targeted removal medical nanoenzyme cleaning agent according to claim 1, characterized in that: The preservative is benzisothiazoline-3-one or chloromethylisothiazolinone.

6. A method for preparing the EPS targeted removal medical nanoenzyme cleaning agent according to any one of claims 1 to 5, characterized in that: The following steps are involved: a. Pour some pure water into the container, add trehalose, glycerol, and sodium xylene sulfonate, and stir until completely dissolved; b. Add defoamer and continue stirring to mix evenly; c. Add nanocomposite enzyme, detergent, EDTA, benzotriazole, preservative and the remaining pure water, continue stirring to mix evenly, add triethanolamine during stirring to adjust the pH value to 7 to 9; d. After aging, the mixture is filtered to obtain the finished product of the EPS targeted removal medical nanoenzyme cleaning agent.

7. The method for preparing the EPS targeted removal medical nanoenzyme cleaning agent according to claim 6, characterized in that: The aging time in step d is 48h to 72h.