Pretreatment device for culturing umbilical cord mesenchymal stem cells

Abstract

The invention relates to the technical field of umbilical cord pretreatment devices, in particular to a pretreatment device for culturing umbilical cord mesenchymal stem cells. According to the technical scheme, the digestion treatment device comprises a supporting cover and a digestion treatment barrel connected to the inner wall of the supporting cover, and further comprises a stirring piece and an opening and closing piece which are arranged in the digestion treatment barrel and used for discharging tissue fluid after digestion and stirring; the side wall of the bottom end of the digestion treatment barrel is in threaded connection with a liquid collecting barrel through a connecting ring, the lower end of the liquid collecting barrel is fixedly connected with a screen, and a transfer centrifugal mechanism is arranged below the screen and used for collecting filtered tissue liquid and conducting centrifugal treatment. According to the design, multiple steps of treatment of the umbilical cord can be continuously completed, the risk of transfer pollution is reduced, after centrifugal treatment, high-purity tissue at the bottom can be taken, next-step culture is facilitated, and after use, the internal structure of the device is convenient to detach, clean and disinfect, and subsequent use is facilitated.

Description

Technical Field

[0001] The present application relates to the technical field of umbilical cord pretreatment devices, and in particular to a pretreatment device for culturing umbilical cord mesenchymal stem cells. Background Art

[0002] Culturing human umbilical cord mesenchymal stem cells requires pretreatment of the umbilical cord. Under sterile conditions, the umbilical cord is separated from the placenta after delivery and quickly transferred to a clean bench. The cord is then cleaned and disinfected, and the outer membrane and blood vessels are removed (carefully using forceps and scissors to separate the cord and blood vessels, leaving the Wharton's jelly intact). The Wharton's jelly is then pulverized. The pulverized tissue is then separated and placed in a solution containing collagenase IV. The digestion is then incubated with agitation for a period of time. The digestion solution is then filtered, the supernatant discarded, and the underlying tissue is seeded into a culture flask. Complete culture medium is added and the tissue is allowed to adhere to the flask.

[0003] When the existing pretreatment device processes the umbilical cord, the umbilical cord digestion, filtration, and separation are completed in different equipment. The umbilical cord tissue needs to be constantly transferred, and great caution is required during operation, otherwise it is easy to cause contamination, which brings great operational pressure to the experimenter; and when the existing centrifugal supernatant discarding equipment is used for processing, the centrifuge tube is first removed, and then the supernatant solution is pumped away, leaving the lower layer tissue. However, when removing the tube and pumping out the supernatant solution, the lower layer tissue is easily disturbed, making it difficult to obtain pure lower layer tissue, which will affect the culture effect.

[0004] To this end, the present application proposes a pretreatment device for culturing umbilical cord mesenchymal stem cells. Summary of the Invention

[0005] The purpose of this application is to address the technical problems pointed out in the background art and to propose a pretreatment device for culturing umbilical cord mesenchymal stem cells.

[0006] The technical solution of the present application is a pretreatment device for culturing umbilical cord mesenchymal stem cells, comprising a support cover and a digestion treatment cylinder connected to the inner wall thereof, and further comprising:

[0007] The stirring member and the opening and closing member are arranged in the digestion treatment cylinder, and are used to discharge the tissue fluid after digestion and stirring;

[0008] The bottom side wall of the digestion treatment cylinder is threadedly connected to the liquid collecting cylinder through a connecting ring, the lower end of the liquid collecting cylinder is fixedly connected to a screen, and a transfer centrifuge mechanism is provided below the screen to collect the filtered tissue fluid and centrifuge it. The inner wall of the support cover is fixedly connected to a positioning ring, and the inner ring of the positioning ring is rotatably connected to a convex ring. The inner wall of the upper end of the convex ring is provided with a sealing mechanism for sealing the tissue fluid during centrifugal treatment of the transfer centrifuge mechanism.

[0009] Preferably, a protective cover is fixedly connected to the middle of the bottom end of the digestion treatment cylinder, and the stirring member includes a stirring motor installed on the inner side of the protective cover. The output shaft of the stirring motor passes through the bottom end of the digestion treatment cylinder and is fixedly connected to a rotating shaft. The upper end of the rotating shaft is fixedly connected to a transmission disk, and a plurality of stirring rods are fixedly connected to the outer lower surface of the transmission disk.

[0010] Preferably, the opening and closing member includes a horizontal plate connected to the inner wall of the upper end of the digestion treatment cylinder, an electric cylinder is installed on the lower surface of the horizontal plate, the telescopic end of the electric cylinder is fixedly connected to the arc-shaped plug plate, the inverted U-shaped rod cover is arranged on the outside of the stirring member, the inner wall of the lower end of the digestion treatment cylinder is provided with two arc-shaped grooves, the two arc-shaped grooves are plugged with arc-shaped plug plates, and the lower ends of both sides of the inverted U-shaped rod are fixedly connected to the two arc-shaped plug plates respectively;

[0011] The threaded cover on the upper end of the digestion treatment cylinder is provided with an upper cover, and the upper end of the upper cover is fixedly connected with an operating block.

[0012] Preferably, a pair of windows are provided on the side wall of the support cover, and the positioning ring is located at the upper end of the opening and closing member;

[0013] The sealing mechanism includes several bumps fixedly connected to the inner wall of the upper end of the convex ring, and the bottom ends of the several bumps are installed with small cylinders. The lower ends of the small cylinders are fixedly connected to threaded connecting rods, and the lower ends of the threaded connecting rods are threadedly connected to sealing plugs.

[0014] Preferably, a servo motor is installed on the inner wall of the lower end of the support cover, the output shaft of the servo motor is fixedly connected to a cylinder, the telescopic end of the cylinder is fixedly connected to a rectangular block, and the transfer centrifugal mechanism is detachably installed between the rectangular block and the convex ring.

[0015] Preferably, the transfer centrifuge mechanism includes a support rod, a rectangular groove is opened at the bottom end of the support rod, the rectangular groove is clamped on the outside of the rectangular block, the upper end of the support rod is fixedly connected to a liquid transfer cylinder, and the upper end of the liquid transfer cylinder is in contact with the lower end surface of the convex ring.

[0016] Preferably, the transfer centrifugal mechanism further comprises a plurality of leakage holes provided at the lower end of the liquid transfer cylinder, the bottom ends of the plurality of leakage holes are fixedly connected to centrifuge tubes, and a plurality of sealing plugs are respectively located directly above the plurality of leakage holes;

[0017] A conical cover is fixedly connected to the middle part of the inner wall of the lower end of the liquid transfer cylinder to guide the tissue fluid in the liquid transfer cylinder so that the tissue fluid can pass through the plurality of leakage holes smoothly.

[0018] Preferably, the bottom ends of the plurality of centrifuge tubes are each provided with a through opening, the lower end of the through opening is threadedly connected to a bottom cover, and the middle portion of the lower end of the bottom cover is a soft plastic film.

[0019] Compared with the prior art, this application has the following beneficial technical effects:

[0020] The present application provides a digestion treatment cylinder inside the support cover, an opening and closing member inside the digestion treatment cylinder, and a liquid collection cylinder detachably connected to the bottom end of the digestion treatment cylinder. After the tissue is digested and stirred in the digestion treatment cylinder, the tissue fluid flows into the liquid collection cylinder through two arc-shaped grooves and is then filtered through a screen. In the process from digestion treatment to filtration, there is no need to transfer tissue fluid, which reduces the probability of tissue fluid contamination and reduces the difficulty of operation.

[0021] By detachably setting a transfer centrifugal mechanism between the convex ring and the rectangular block, the transfer cylinder can transfer the filtered tissue fluid into a plurality of centrifuge tubes. After the tissue fluid is filtered and transferred, the plurality of leakage holes are sealed by the sealing mechanism, and then the centrifugal treatment is carried out, thereby achieving a pollution-free treatment effect with high efficiency.

[0022] By opening a through hole at the bottom of the centrifuge tube and connecting the outer side of the through hole to the bottom cover, after the centrifugation process is completed, the bottom cells can be directly extracted with a syringe through the soft plastic film at the bottom of the bottom cover, which can ensure the purity of the cells and thus the culture effect. After the process is completed, the transfer centrifuge mechanism, sealing plug, and liquid collection cylinder can be disassembled in sequence for cleaning and disinfection, which can be thoroughly disinfected and convenient for subsequent use.

[0023] In summary, the design of the present application can complete multiple steps of umbilical cord processing in a coherent manner, reducing the risk of transfer contamination. After centrifugation, high-purity tissue can be obtained from the bottom, which is beneficial for the next step of culture. After use, the internal structure of the device is easy to disassemble, clean and disinfect, making it convenient for subsequent use. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is a perspective view of a pretreatment device for culturing umbilical cord mesenchymal stem cells;

[0025] Figure 2 This application Figure 1 A front sectional view of

[0026] Figure 3 It is a schematic diagram of the internal structure of the transfer liquid cylinder in this application;

[0027] Figure 4 yes Figure 3 Schematic diagram of the bottom structure;

[0028] Figure 5 yes Figure 2 A in the middle is an enlarged structural diagram;

[0029] Figure 6 It is a schematic diagram of the structure of the opening and closing member and the stirring member in this application;

[0030] Figure 7 It is a schematic diagram of the bottom structure of the digestion treatment cylinder in this application.

[0031] Reference numerals: 1, support cover; 2, upper cover; 3, operating block; 4, digestion treatment cylinder; 5, positioning ring;

[0032] 6. Opening and closing parts; 61. Horizontal plate; 62. Electric cylinder; 63. Inverted U-shaped rod; 64. Arc plug plate; 65. Arc groove;

[0033] 7. stirring member; 71. rotating shaft; 72. transmission plate; 73. stirring rod; 74. stirring motor;

[0034] 8. Protective cover; 9. Connecting ring; 10. Liquid collecting cylinder; 11. Screen; 12. Raised ring;

[0035] 13. Sealing mechanism; 131. Bump; 132. Small cylinder; 133. Threaded connecting rod; 134. Sealing plug;

[0036] 14. Servo motor; 15. Cylinder; 16. Rectangular block;

[0037] 17. Transfer centrifuge mechanism; 171. Liquid transfer cylinder; 172. Conical cover; 173. Leakage hole; 174. Centrifuge tube; 175. Bottom cover; 176. Support rod; 177. Rectangular groove; 18. Window. DETAILED DESCRIPTION

[0038] The technical solution of the present application is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0039] The components of the embodiments of the present application generally described and shown in the drawings herein may be arranged and designed in a variety of different configurations. Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed application, but merely represents selected embodiments of the present application.

[0040] Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making any creative work shall fall within the scope of protection of this application.

[0041] In the description of this application, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," "the other end," and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate description and simplify the description of the present invention. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0042] In the description of this application, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal connections between two components. Those skilled in the art will understand the specific meanings of the above terms in this application based on the specific circumstances.

[0043] Example

[0044] like Figure 1-Figure 7 As shown, the present application proposes a pretreatment device for culturing umbilical cord mesenchymal stem cells, comprising a support cover 1 and a digestion treatment cylinder 4 connected to the inner wall thereof, wherein the upper end of the digestion treatment cylinder 4 is fixedly connected to a threaded sleeve protruding from the upper end surface of the support cover 1, and the upper end threaded cover of the digestion treatment cylinder 4 is provided with an upper cover 2, and the upper end of the upper cover 2 is fixedly connected to an operating block 3.

[0045] It also includes a stirring member 7 and an opening and closing member 6 arranged in the digestion treatment cylinder 4, which are used to discharge the tissue fluid after digestion and stirring. The bottom side wall of the digestion treatment cylinder 4 is threadedly connected to a liquid collecting cylinder 10 through a connecting ring 9, and the liquid collecting cylinder 10 can be unscrewed downward. The lower end of the liquid collecting cylinder 10 is fixedly connected to a screen 11, and a transfer centrifugal mechanism 17 is provided below the screen 11 for collecting the filtered tissue fluid and centrifuging it. The inner wall of the support cover 1 is fixedly connected to a positioning ring 5, and a pair of windows 18 are provided on the side wall of the support cover 1. The positioning ring 5 is located at the upper end of the opening and closing member 6. The inner ring of the positioning ring 5 is rotatably connected to a convex ring 12, and the inner wall of the upper end of the convex ring 12 is provided with a sealing mechanism 13, which is used to seal the tissue fluid during centrifugal treatment of the transfer centrifugal mechanism 17 to ensure the effect and safety of the centrifugation.

[0046] Specifically, a protective cover 8 is fixedly connected to the middle portion of the bottom end of the digestion treatment drum 4. The stirring member 7 includes a stirring motor 74 mounted inside the protective cover 8. The output shaft of the stirring motor 74 passes through the bottom end of the digestion treatment drum 4 and is fixedly connected to a rotating shaft 71. The upper end of the rotating shaft 71 is fixedly connected to a transmission disk 72, and the outer lower surface of the transmission disk 72 is fixedly connected to a plurality of stirring rods 73. The stirring motor 74 is electrically connected to an external controller. By starting the stirring motor 74, the rotating shaft 71 rotates, which in turn drives the plurality of stirring rods 73 to stir the tissue fluid in the digestion treatment drum 4, thereby fully digesting the tissue fluid.

[0047] Among them, the opening and closing part 6 includes a horizontal plate 61 connected to the inner wall of the upper end of the digestion treatment cylinder 4, and an electric cylinder 62 is installed on the lower surface of the horizontal plate 61. The telescopic end of the electric cylinder 62 is fixedly connected to the arc plug plate 64, and the inverted U-shaped rod 63 is covered on the outside of the stirring member 7. Two arc grooves 65 are provided on the inner wall of the lower end of the digestion treatment cylinder 4, and arc plug plates 64 are inserted into the two arc grooves 65. The lower ends of both sides of the inverted U-shaped rod 63 are fixedly connected to the two arc plug plates 64 respectively; wherein, the two arc plug plates 64 are rubber plugs with a good sealing effect.

[0048] The sealing mechanism 13 includes a plurality of protrusions 131 fixedly connected to the inner wall of the upper end of the convex ring 12, and a small cylinder 132 is installed at the bottom end of each of the protrusions 131. The lower end of the small cylinder 132 is fixedly connected to a threaded connecting rod 133, and the lower end of the threaded connecting rod 133 is threadedly connected to a sealing plug 134. After the umbilical cord processing is completed, the sealing plug 134 is conveniently removed for cleaning and disinfection.

[0049] Furthermore, a servo motor 14 is mounted on the inner wall of the lower end of the support cover 1. The output shaft of the servo motor 14 is fixedly connected to a cylinder 15. The telescopic end of the cylinder 15 is fixedly connected to a rectangular block 16. The transfer centrifugal mechanism 17 is detachably mounted between the rectangular block 16 and the convex ring 12. The transfer centrifugal mechanism 17 includes a support rod 176. The bottom end of the support rod 176 is provided with a rectangular groove 177. The rectangular groove 177 is snap-fitted to the outer side of the rectangular block 16. The upper end of the support rod 176 is fixedly connected to a liquid transfer cylinder 171. The upper end of the liquid transfer cylinder 171 contacts the lower end surface of the convex ring 12. When the liquid transfer cylinder 171 rotates, the convex ring 12 rotates synchronously therewith. The transfer centrifuge mechanism 17 also includes a plurality of leakage holes 173 formed at the lower end of a transfer cylinder 171. Centrifuge tubes 174 are fixedly connected to the bottom ends of the plurality of leakage holes 173. A plurality of sealing plugs 134 are located directly above the plurality of leakage holes 173. A conical cover 172 is fixedly connected to the middle portion of the inner wall of the lower end of the transfer cylinder 171 to guide the tissue fluid within the transfer cylinder 171 so that the tissue fluid passes smoothly through the plurality of leakage holes 173. The bottom ends of the plurality of centrifuge tubes 174 are each provided with a through opening, the lower ends of which are threadedly connected to a bottom cover 175. The middle portion of the lower end of the bottom cover 175 is a soft plastic sheet. The transfer cylinder 171 can transfer the filtered tissue fluid into several centrifuge tubes 174. After the tissue fluid is filtered and transferred, the several leakage holes 173 are sealed by the sealing mechanism 13, and then centrifugation is performed to achieve a pollution-free treatment effect; by opening a through hole at the bottom end of the centrifuge tube 174, the outer side of the through hole is connected to the bottom cover 175. After the centrifugation is completed, a syringe can be used to directly penetrate the soft plastic film at the bottom end of the bottom cover 175 to extract the bottom cells, which can ensure the purity of the cells and thus ensure the effect of cell culture.

[0050] The operating principle of this embodiment is as follows: first, the upper cover 2 is opened, and then the pulverized umbilical cord tissue is added to the digestion treatment cylinder 4 of the support cover 1. A solution, which may be a collagenase IV solution, is then added to the digestion treatment cylinder 4. The upper cover 2 is then closed, and the stirring motor 74 is activated. The output shaft of the stirring motor 74 rotates, driving the rotating shaft 71. The rotation of the rotating shaft 71 drives several stirring rods 73 to stir the digestion treatment cylinder 4, ensuring that the umbilical cord tissue and the solution are fully mixed and contacted, completing the enzymatic digestion treatment. After the enzymatic digestion treatment, the stirring motor 74 is turned off, and the electric cylinder 62 is activated. The telescopic end of the electric cylinder 62 is retracted, driving the inverted U-shaped rod 63 to move upward. The inverted U-shaped rod 63 then drives the two arcuate plug plates 64 to leave the two arcuate grooves 65, allowing the tissue solution in the digestion treatment cylinder 4 to flow through the two arcuate grooves 65 into the liquid collection cylinder 10, where the tissue solution is filtered through the screen 11.

[0051] The tissue solution filtered by the screen 11 falls directly into the transfer cylinder 171. The tissue solution in the transfer cylinder 171 flows into the centrifuge tubes 174 through the leakage holes 173. When all the tissue solution has flowed into the centrifuge tubes 174, the small cylinders 132 are started. The telescopic ends of the small cylinders 132 extend and drive the sealing plugs 134 to move downward a certain distance. The plugs are respectively inserted into the leakage holes 173 to seal the centrifuge tubes 174.

[0052] Next, the servo motor 14 is started. The output shaft of the servo motor 14 rotates through the cylinder 15, driving the rectangular block 16 to rotate. This in turn drives the liquid transfer cylinder 171 to rotate through the support rod 176, causing the multiple centrifuge tubes 174 to rotate, completing the centrifugal treatment of the tissue solution. Due to the different densities of different substances in the tissue solution, under the continuous action of centrifugal force, they will gradually separate into different layers. The density of cells is greater than that of the solution, and they will settle to the bottom of the centrifuge tube 174 to form a precipitate, while the solution remains in the upper layer.

[0053] After the centrifugation is completed, the telescopic ends of the several small cylinders 132 are retracted, driving the several sealing plugs 134 to leave the several leakage holes 173, and then a sterile syringe is used to directly penetrate the soft plastic film at the bottom end of the bottom cover 175 to extract the bottom layer cells, which can ensure the purity of the cells and thus ensure the culture effect. During the process of extracting cells, there is no need to move the centrifuge tube 174 or to extract the supernatant, thereby effectively avoiding the disturbance of the solution after separation.

[0054] After the treatment is completed, the transfer centrifugal mechanism 17, the sealing plug 134, and the liquid collecting cylinder 10 can be disassembled in sequence for cleaning and disinfection. They can be thoroughly disinfected to facilitate subsequent use. The specific disassembly steps are: first, retract the telescopic end of the cylinder 15, and the entire transfer centrifugal mechanism 17 falls downward, so that the upper end of the liquid transfer cylinder 171 is separated from the convex ring 12 by a certain distance, and then the transfer centrifugal mechanism 17 is manipulated through the window 18, and the transfer centrifugal mechanism 17 is moved upward by a certain distance to disengage the rectangular block 16 from the rectangular groove 177, and then the transfer centrifugal mechanism 17 is taken out to one side. After the transfer centrifugal mechanism 17 is removed, the sealing plug 134 is unscrewed in turn, and the liquid collecting cylinder 10 is unscrewed in the next step. Each structure can be fully cleaned and disinfected.

[0055] The above specific embodiments are merely preferred embodiments of the present application. Based on the technical solutions of the present application and the relevant inspirations of the above embodiments, those skilled in the art may make various alternative improvements and combinations of the above specific embodiments. The above specific embodiments are merely explanations of the present application and are not limitations of the present application.

Claims

1. A pretreatment device for culturing umbilical cord mesenchymal stem cells, comprising a support cover (1) and a digestion treatment cylinder (4) connected to the inner wall thereof, characterized in that: Also includes: The stirring member (7) and the opening and closing member (6) are arranged in the digestion treatment cylinder (4) and are used to discharge tissue fluid after digestion and stirring; The bottom side wall of the digestion treatment cylinder (4) is threadedly connected to a liquid collecting cylinder (10) through a connecting ring (9), and the lower end of the liquid collecting cylinder (10) is fixedly connected to a screen (11). A transfer centrifuge mechanism (17) is provided below the screen (11) for collecting filtered tissue fluid and centrifuging it. The inner wall of the support cover (1) is fixedly connected to a positioning ring (5), and the inner ring of the positioning ring (5) is rotatably connected to a convex ring (12). The inner wall of the upper end of the convex ring (12) is provided with a sealing mechanism (13) for sealing the tissue fluid during centrifugal treatment of the transfer centrifuge mechanism (17).

2. A pretreatment device for culturing umbilical cord mesenchymal stem cells according to claim 1, characterized in that: A protective cover (8) is fixedly connected to the middle of the bottom end of the digestion treatment cylinder (4), and the stirring member (7) includes a stirring motor (74) installed on the inner side of the protective cover (8). The output shaft of the stirring motor (74) passes through the bottom end of the digestion treatment cylinder (4) and is fixedly connected to a rotating shaft (71). The upper end of the rotating shaft (71) is fixedly connected to a transmission disk (72), and the outer lower surface of the transmission disk (72) is fixedly connected to a plurality of stirring rods (73).

3. A pretreatment device for culturing umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The opening and closing member (6) includes a horizontal plate (61) connected to the inner wall of the upper end of the digestion treatment cylinder (4), an electric cylinder (62) is installed on the lower surface of the horizontal plate (61), and the telescopic end of the electric cylinder (62) is fixedly connected to an arc plug plate (64), and the inverted U-shaped rod (63) is covered on the outside of the stirring member (7). The inner wall of the lower end of the digestion treatment cylinder (4) is provided with two arc grooves (65), and the two arc plug plates (64) are inserted into the two arc grooves (65). The lower ends of both sides of the inverted U-shaped rod (63) are fixedly connected to the two arc plug plates (64). The upper threaded cover of the digestion treatment cylinder (4) is provided with an upper cover (2), and the upper end of the upper cover (2) is fixedly connected to an operating block (3).

4. A pretreatment device for culturing umbilical cord mesenchymal stem cells according to claim 3, characterized in that: A pair of windows (18) are provided on the side wall of the support cover (1), and the positioning ring (5) is located at the upper end of the opening and closing member (6); The sealing mechanism (13) comprises a plurality of protrusions (131) fixedly connected to the inner wall of the upper end of the convex ring (12); a small cylinder (132) is installed at the bottom end of each of the protrusions (131); a threaded connecting rod (133) is fixedly connected to the lower end of the small cylinder (132); and a sealing plug (134) is threadedly connected to the lower end of the threaded connecting rod (133).

5. A pretreatment device for culturing umbilical cord mesenchymal stem cells according to claim 4, characterized in that: A servo motor (14) is installed on the inner wall of the lower end of the support cover (1), the output shaft of the servo motor (14) is fixedly connected to a cylinder (15), the telescopic end of the cylinder (15) is fixedly connected to a rectangular block (16), and the transfer centrifugal mechanism (17) is detachably installed between the rectangular block (16) and the convex ring (12).

6. The pretreatment device for culturing umbilical cord mesenchymal stem cells according to claim 5, characterized in that: The transfer centrifugal mechanism (17) includes a support rod (176), the bottom end of the support rod (176) is provided with a rectangular groove (177), the rectangular groove (177) is clamped on the outside of the rectangular block (16), the upper end of the support rod (176) is fixedly connected to the liquid transfer cylinder (171), and the upper end of the liquid transfer cylinder (171) is in contact with the lower end surface of the convex ring (12).

7. A pretreatment device for culturing umbilical cord mesenchymal stem cells according to claim 6, characterized in that: The transfer centrifugal mechanism (17) further comprises a plurality of leakage holes (173) provided at the lower end of the liquid transfer cylinder (171), the bottom ends of the plurality of leakage holes (173) are fixedly connected to centrifuge tubes (174), and a plurality of sealing plugs (134) are respectively located directly above the plurality of leakage holes (173); A conical cover (172) is fixedly connected to the middle of the inner wall of the lower end of the liquid transfer cylinder (171) to guide the tissue fluid in the liquid transfer cylinder (171) so that the tissue fluid can pass through the plurality of leakage holes (173) smoothly.

8. The pretreatment device for culturing umbilical cord mesenchymal stem cells according to claim 7, characterized in that: The bottom ends of the plurality of centrifuge tubes (174) are all provided with through openings, the lower ends of the through openings are threadedly connected with bottom covers (175), and the middle portion of the lower end of the bottom cover (175) is a soft plastic sheet.

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