Novel hydrogel for promoting cartilage growth and hydrogel 3D printing stent

Abstract

The invention provides a novel hydrogel for promoting cartilage growth, which is prepared by the following steps: S1, stirring and mixing a hyaluronic acid solution, a glucosamine solution and an acrylamide solution in different proportions, and fully reacting; s2, adding a proper amount of cross-linking agent N, N '-methylene bisacrylamide, and stirring to fully react; and S3, adding an initiator into the mixed solution, stirring the mixed solution, and starting a polymerization reaction to form the hydrogel with a cross-linked network structure. The hydrogel prepared by the invention is directly printed into a composite hydrogel artificial bone composite scaffold by adopting a photocuring 3D printing technology, when the composite hydrogel artificial bone composite scaffold is applied to a cartilage injury part, the cell proliferation of the cartilage injury part is obvious, and the connectivity between the hydrogel scaffold and cartilage is stronger, so that the hydrogel scaffold has a good promotion effect on cartilage injury repair, and the cartilage injury repair effect is better. And no toxic substance deposition is found in cartilage injury.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a novel hydrogel that promotes cartilage growth and a hydrogel 3D printing scaffold. Background Art

[0002] Currently, cartilage repair remains a significant challenge for orthopedic surgeons. Cartilage tissue engineering scaffolds fabricated using 3D printing technology offer the advantages of personalization and diversification, and hold great promise for development in the field of orthopedic medical devices.

[0003] Although hydrogels are new materials with great advantages and application potential in cartilage tissue engineering, the existing hydrogel preparation methods and processes are different. Due to the complexity of cartilage damage repair, the various existing medical hydrogels cannot meet the requirements of use. Therefore, we propose a new hydrogel that promotes cartilage growth and a hydrogel 3D printed scaffold to solve the above problems. Summary of the Invention

[0004] (1) Technical problems solved

[0005] In response to the shortcomings of the existing technology, the present invention provides a new hydrogel that promotes cartilage growth and a hydrogel 3D printing scaffold, which solves the problem that existing medical hydrogels cannot be put into practical application.

[0006] (2) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: A new hydrogel that promotes cartilage growth includes the following preparation steps:

[0008] S1. Stirring and mixing hyaluronic acid solution, glucosamine solution and acrylamide solution in different proportions to allow them to fully react;

[0009] S2, then add an appropriate amount of cross-linking agent N, N'-methylenebisacrylamide and stir to allow it to react fully;

[0010] S3, adding an initiator to the mixed solution, stirring the mixed solution, starting a polymerization reaction, and forming a hydrogel with a cross-linked network structure;

[0011] S4, pouring the reaction solution into a mold and allowing it to stand to complete gelation;

[0012] S5, removing the hydrogel from the mold and washing it to remove unreacted monomers and impurities;

[0013] S6. The washed hydrogel is vacuum dried to obtain a dry hydrogel material.

[0014] Preferably, the hyaluronic acid solution in S1 is prepared by dissolving 1 g of hyaluronic acid in 100 ml of deionized water.

[0015] Preferably, the glucosamine solution in S1 is prepared by dissolving 4 g of glucosamine in 100 ml of deionized water.

[0016] Preferably, the concentration of the acrylamide solution in S1 is 10%.

[0017] Preferably, the ratios of the hyaluronic acid solution, glucosamine solution and acrylamide solution in S1 are 10:40:50, 25:25:50 and 40:10:50, respectively.

[0018] Preferably, the mass of the cross-linking agent N,N'-methylenebisacrylamide in S2 is 0.3% of the total mass of acrylamide.

[0019] Preferably, the initiators in S3 are ammonium persulfate and tetramethylethylenediamine, and the added mass of the ammonium persulfate is 0.1 g.

[0020] A new hydrogel 3D printed scaffold that promotes cartilage growth is prepared using a new hydrogel that promotes cartilage growth.

[0021] (3) Beneficial effects

[0022] The present invention provides a novel hydrogel and hydrogel 3D printing scaffold for promoting cartilage growth. It has the following beneficial effects:

[0023] The hydrogel prepared by the present invention is directly printed using light-curing 3D printing technology to form a composite hydrogel artificial bone composite scaffold. When used at the site of cartilage damage, cell proliferation at the site of cartilage damage is obvious, and the connectivity between the hydrogel scaffold and the cartilage is stronger, indicating that the hydrogel scaffold has a good promoting effect on cartilage damage repair, and no toxic substance deposition is found in the cartilage damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a physical picture of a new hydrogel for promoting cartilage growth proposed by the present invention;

[0025] Figure 2 This is a physical picture of Type I of a new hydrogel 3D printed scaffold for promoting cartilage growth proposed by the present invention;

[0026] Figure 3 This is a physical picture of Type II of a new hydrogel 3D printed scaffold for promoting cartilage growth proposed in the present invention. DETAILED DESCRIPTION

[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0028] Example 1:

[0029] like Figure 1-3 As shown, the embodiment of the present invention provides a novel hydrogel for promoting cartilage growth, comprising the following preparation steps:

[0030] S1. Take 10 ml of hyaluronic acid solution, 40 ml of glucosamine solution, and 50 ml of acrylamide solution, stir and mix them to fully react, wherein the hyaluronic acid solution is prepared by dissolving 1 g of hyaluronic acid in 100 ml of deionized water, the glucosamine solution is prepared by dissolving 4 g of glucosamine in 100 ml of deionized water, and the concentration of the acrylamide solution is 10%;

[0031] S2, adding 0.3% of the total mass of acrylamide as a cross-linking agent N,N'-methylenebisacrylamide, stirring to allow for full reaction;

[0032] S3, adding 0.1g of ammonium persulfate to the mixed solution, stirring the mixed solution, and adding tetramethylethylenediamine while stirring to start a polymerization reaction to form a hydrogel with a cross-linked network structure, and stopping the addition of tetramethylethylenediamine until no more hydrogel can be formed;

[0033] S4, pouring the reaction solution into a mold and allowing it to stand to complete gelation;

[0034] S5. removing the hydrogel from the mold and washing it to remove unreacted monomers and impurities, washing it with deionized water for at least three times;

[0035] S6. The washed hydrogel is vacuum dried to obtain a dry hydrogel material.

[0036] The dried hydrogel material was made into a scaffold that can be used for cartilage damage repair using 3D printing technology. A cartilage damage repair experiment was carried out on experimental rabbits, and the cartilage damage repair of the rabbits was observed throughout the process.

[0037] Example 2:

[0038] like Figure 1-3 As shown, the embodiment of the present invention provides a novel hydrogel for promoting cartilage growth, comprising the following preparation steps:

[0039] S1. Take 25 ml of hyaluronic acid solution, 25 ml of glucosamine solution, and 50 ml of acrylamide solution, stir and mix them to fully react, wherein the hyaluronic acid solution is prepared by dissolving 1 g of hyaluronic acid in 100 ml of deionized water, the glucosamine solution is prepared by dissolving 4 g of glucosamine in 100 ml of deionized water, and the concentration of the acrylamide solution is 10%;

[0040] S2, adding 0.3% of the total mass of acrylamide as a cross-linking agent N,N'-methylenebisacrylamide, stirring to allow for full reaction;

[0041] S3, adding 0.1g of ammonium persulfate to the mixed solution, stirring the mixed solution, and adding tetramethylethylenediamine while stirring to start a polymerization reaction to form a hydrogel with a cross-linked network structure, and stopping the addition of tetramethylethylenediamine until no more hydrogel can be formed;

[0042] S4, pouring the reaction solution into a mold and allowing it to stand to complete gelation;

[0043] S5. removing the hydrogel from the mold and washing it to remove unreacted monomers and impurities, washing it with deionized water for at least three times;

[0044] S6. The washed hydrogel is vacuum dried to obtain a dry hydrogel material.

[0045] The dried hydrogel material was made into a scaffold that can be used for cartilage damage repair using 3D printing technology. A cartilage damage repair experiment was carried out on experimental rabbits, and the cartilage damage repair of the rabbits was observed throughout the process.

[0046] Example 3:

[0047] like Figure 1-3 As shown, the embodiment of the present invention provides a novel hydrogel for promoting cartilage growth, comprising the following preparation steps:

[0048] S1. Take 40 ml of hyaluronic acid solution, 10 ml of glucosamine solution, and 50 ml of acrylamide solution, stir and mix them to fully react, wherein the hyaluronic acid solution is prepared by dissolving 1 g of hyaluronic acid in 100 ml of deionized water, the glucosamine solution is prepared by dissolving 4 g of glucosamine in 100 ml of deionized water, and the concentration of the acrylamide solution is 10%;

[0049] S2, adding 0.3% of the total mass of acrylamide as a cross-linking agent N,N'-methylenebisacrylamide, stirring to allow for full reaction;

[0050] S3, adding 0.1g of ammonium persulfate to the mixed solution, stirring the mixed solution, and adding tetramethylethylenediamine while stirring to start a polymerization reaction to form a hydrogel with a cross-linked network structure, and stopping the addition of tetramethylethylenediamine until no more hydrogel can be formed;

[0051] S4, pouring the reaction solution into a mold and allowing it to stand to complete gelation;

[0052] S5. removing the hydrogel from the mold and washing it to remove unreacted monomers and impurities, washing it with deionized water for at least three times;

[0053] S6. The washed hydrogel is vacuum dried to obtain a dry hydrogel material.

[0054] The dried hydrogel material was made into a scaffold that can be used for cartilage damage repair using 3D printing technology. A cartilage damage repair experiment was carried out on experimental rabbits, and the cartilage damage repair of the rabbits was observed throughout the process.

[0055] Table 1 below is a comparison of the hydrogel scaffolds prepared using different proportions of components according to the present invention during use:

[0056] Table 1

[0057] Tensile properties Cell proliferation Connectivity Toxic deposition Example 1 Strong Faster powerful none Example 1 powerful quick powerful none Example 1 powerful Faster Strong none

[0058] As can be seen from Table 1, the hydrogel scaffold prepared by the present invention using hyaluronic acid solution, glucosamine solution and acrylamide solution, in combination with the cross-linking agent N,N'-methylenebisacrylamide, and the initiators ammonium persulfate and tetramethylethylenediamine can be actually used in cartilage damage sites. Cell proliferation at the cartilage damage site is obvious, and the connectivity between the hydrogel scaffold and the cartilage is stronger, indicating that the hydrogel scaffold has a good promoting effect on cartilage damage repair, and no toxic substance deposition was found in the cartilage damage.

[0059] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A novel hydrogel for promoting cartilage growth, characterized by: The method comprises the following preparation steps: S1. Stirring and mixing hyaluronic acid solution, glucosamine solution and acrylamide solution in different proportions to allow them to fully react; S2, then add an appropriate amount of cross-linking agent N, N'-methylenebisacrylamide and stir to allow it to react fully; S3, adding an initiator to the mixed solution, stirring the mixed solution, starting a polymerization reaction, and forming a hydrogel with a cross-linked network structure; S4, pouring the reaction solution into a mold and allowing it to stand to complete gelation; S5, removing the hydrogel from the mold and washing it to remove unreacted monomers and impurities; S6. The washed hydrogel is vacuum dried to obtain a dry hydrogel material.

2. The novel hydrogel for promoting cartilage growth according to claim 1, characterized in that: The hyaluronic acid solution in S1 was prepared by dissolving 1 g of hyaluronic acid in 100 ml of deionized water.

3. The novel hydrogel for promoting cartilage growth according to claim 1, characterized in that: The glucosamine solution in S1 was prepared by dissolving 4 g of hyaluronic acid in 100 ml of deionized water.

4. The novel hydrogel for promoting cartilage growth according to claim 1, characterized in that: The concentration of the acrylamide solution in S1 is 10%.

5. The novel hydrogel for promoting cartilage growth according to claim 1, characterized in that: The ratios of the hyaluronic acid solution, glucosamine solution and acrylamide solution in S1 are 10: 40:50, 25:25:50 and 40:10:

50.

6. The novel hydrogel for promoting cartilage growth according to claim 1, characterized in that: The mass of the cross-linking agent N,N'-methylenebisacrylamide in S2 is 0.3% of the total mass of acrylamide.

7. The novel hydrogel for promoting cartilage growth according to claim 1, characterized in that: The initiators in S3 are ammonium persulfate and tetramethylethylenediamine, and the added mass of the ammonium persulfate is 0.1 g.

8. A novel hydrogel 3D printing scaffold for promoting cartilage growth, prepared using the novel hydrogel for promoting cartilage growth according to any one of claims 1 to 7.

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