Santalum album saja2l protein and / or saja2l gene and its use in promoting santalum album essential oil synthesis

Abstract

The application discloses sandalwood SaJA2L protein and / or SaJA2L gene and application thereof in promoting synthesis of sandalwood essential oil, wherein the amino acid sequence of the sandalwood SaJA2L protein is shown as SEQ ID NO. 2. The application firstly obtains the SaJA2L gene in sandalwood, and discloses the regulation effect of the gene in natural sandalwood essential oil synthesis, and overexpression of the SaJA2L gene in sandalwood can significantly promote synthesis of the sandalwood essential oil. The application provides a new technical selection for improving yield of natural sandalwood essential oil, and has important popularization and application value in cultivating new sandalwood varieties with high essential oil synthesis efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of plant breeding, and in particular to a sandalwood SaJA2L protein and / or a SaJA2L gene and an application thereof in promoting the synthesis of sandalwood essential oil. Background Art

[0002] sandalwood( Santalum album Sandalwood (Sandalwood L.) is a plant of immense economic value and cultural significance. Sandalwood essential oil, with its unique aroma and diverse bioactivities, including antibacterial, anti-inflammatory, and antidepressant properties, is widely used in high-end perfumes, cosmetics, pharmaceuticals, and traditional fragrances. However, due to its slow growth and demanding ecological requirements, natural sandalwood resources are extremely limited. This has resulted in an inability to meet market demand, keeping its price high and restricting its wider application.

[0003] The main components of sandalwood essential oil are santalene (α-santalene and β-santalene), and santalol (α-santalol and β-santalol). These compounds give sandalwood its signature aroma and play a key role in various biological activities. The synthesis of natural sandalwood essential oil is complex, beginning with the conversion of farnesyl diphosphate (FPP) to santalene by santalene synthase SaSSY (a member of the terpene synthase TPS family), followed by catalysis by the P450 oxidase SaCYP736A167 to santalol. To alleviate the shortage of natural sandalwood essential oil, various chemical synthesis techniques have emerged in recent years, allowing for the production of sandalwood essential oil through artificial synthesis. However, current synthetic techniques struggle to replicate the entire natural essential oil synthesis process, resulting in significant differences in aroma and biological activity. This leads to low market acceptance, high production costs, and environmental pollution, making it a poor substitute for natural sandalwood essential oil. How to increase the yield of natural sandalwood essential oil remains a key problem that technicians in this field urgently need to overcome. Summary of the Invention

[0004] In order to solve the above problems existing in the prior art, the present invention provides a sandalwood SaJA2L protein and / or a SaJA2L gene and their use in promoting the synthesis of sandalwood essential oil.

[0005] The first object of the present invention is to provide sandalwood SaJA2L protein.

[0006] The second object of the present invention is to provide the use of sandalwood SaJA2L protein and / or SaJA2L gene in promoting the synthesis of sandalwood essential oil.

[0007] The third object of the present invention is to provide the use of sandalwood SaJA2L protein and / or SaJA2L gene in promoting the synthesis of santalene and / or santalol.

[0008] A fourth object of the present application is to provide a biological material for promoting the synthesis of sandalwood essential oil.

[0009] A fifth object of the present application is to provide the use of the biological material in promoting the synthesis of sandalwood essential oil.

[0010] A sixth object of the present application is to provide a product for promoting the synthesis of sandalwood essential oil.

[0011] A seventh object of the present application is to provide a method for promoting the synthesis of sandalwood essential oil.

[0012] In order to achieve the above objects, the present application is implemented by the following solutions:

[0013] The present application finds a SaJA2L gene in sandalwood, which encodes a SaJA2L protein, and successfully overexpresses the SaJA2L gene in sandalwood through tissue culture and Agrobacterium vacuum infiltration transformation method, and determines the regulation function of the SaJA2L gene in the synthesis of sandalwood essential oil.

[0014] The present application claims the following contents:

[0015] Sandalwood SaJA2L protein, the amino acid sequence of which is shown as SEQ ID NO. 2.

[0016] The use of sandalwood SaJA2L protein and / or SaJA2L gene in promoting the synthesis of sandalwood essential oil, the amino acid sequence of the sandalwood SaJA2L protein being shown as SEQ ID NO. 2.

[0017] Preferably, the sandalwood essential oil is sandalwood essential oil of Indian old mountain sandalwood.

[0018] The use of sandalwood SaJA2L protein and / or SaJA2L gene in promoting the synthesis of sandalene and / or sandalol, the amino acid sequence of the sandalwood SaJA2L protein being shown as SEQ ID NO. 2.

[0019] Preferably, the sandalene and / or sandalol is sandalene and / or sandalol of Indian old mountain sandalwood.

[0020] More preferably, the sandalene includes alpha-sandalene and beta-sandalene.

[0021] More preferably, the sandalol includes alpha-sandalol and beta-sandalol.

[0022] The use of an agent for promoting the expression of sandalwood SaJA2L protein and / or SaJA2L gene in promoting the synthesis of sandalwood essential oil, the amino acid sequence of the sandalwood SaJA2L protein being shown as SEQ ID NO. 2.

[0023] Preferably, the sandalwood essential oil is sandalwood essential oil from Indian sandalwood.

[0024] Use of an agent for promoting the expression of sandalwood SaJA2L protein and / or SaJA2L gene in promoting the synthesis of santalene and / or santalol, wherein the amino acid sequence of the sandalwood SaJA2L protein is shown in SEQ ID NO.2.

[0025] Preferably, the santalene and / or santalol are santalene and / or santalol from Indian sandalwood.

[0026] More preferably, the santalene includes α-santalene and β-santalene.

[0027] More preferably, the santalol includes α-santalol and β-santalol.

[0028] A biomaterial for promoting the synthesis of sandalwood essential oil, which is any one of the following (1) to (4):

[0029] (1) a nucleic acid molecule encoding a sandalwood SaJA2L protein, the amino acid sequence of which is shown in SEQ ID NO. 2;

[0030] (2) an expression cassette containing the nucleic acid molecule described in (1);

[0031] (3) a recombinant expression vector containing the nucleic acid molecule described in (1);

[0032] (4) A microorganism containing the recombinant expression vector described in (3).

[0033] Preferably, the nucleotide sequence of the nucleic acid molecule in (1) is as shown in SEQ ID NO.1.

[0034] Preferably, the recombinant expression vector described in (3) uses the PBWA(V)HS-GFP vector as its backbone.

[0035] More preferably, the recombinant expression vector in (3) uses the PBWA(V)HS-GFP vector as a backbone, and the nucleic acid molecule in (1) is located between the SmaI site and the XbaI site.

[0036] The present invention has no special limitation on the source and type of the microorganisms described in (4), including but not limited to Escherichia coli, Agrobacterium and other conventional microorganisms that can carry the recombinant expression vector can achieve the purpose of the present invention.

[0037] Preferably, the microorganism in (4) is Agrobacterium.

[0038] More preferably, the Agrobacterium is Agrobacterium tumefaciens.

[0039] Further preferably, the Agrobacterium tumefaciens is LBA4404 strain.

[0040] Use of any of the biological materials in promoting synthesis of sandalwood oil.

[0041] Preferably, the sandalwood oil is sandalwood oil of Santalum album L.

[0042] Use of any of the biological materials in promoting synthesis of santalene and / or santalol.

[0043] Preferably, the sandalwood oil is santalene and / or santalol of Santalum album L.

[0044] More preferably, the santalene comprises α-santalene and β-santalene.

[0045] More preferably, the santalol comprises α-santalol and β-santalol.

[0046] A product for promoting synthesis of sandalwood oil, comprising a biological material for promoting synthesis of sandalwood oil, the biological material being any of the following (1) to (4):

[0047] (1) a nucleic acid molecule encoding sandalwood SaJA2L protein, the amino acid sequence of the sandalwood SaJA2L protein being shown as SEQ ID NO. 2;

[0048] (2) an expression cassette containing the nucleic acid molecule in (1);

[0049] (3) a recombinant expression vector containing the nucleic acid molecule in (1);

[0050] (4) a microorganism containing the recombinant expression vector in (3).

[0051] Preferably, the nucleotide sequence of the nucleic acid molecule in (1) is shown as SEQ ID NO. 1.

[0052] Preferably, the recombinant expression vector in (3) takes PBWA(V)HS-GFP vector as a skeleton.

[0053] More preferably, the recombinant expression vector in (3) takes PBWA(V)HS-GFP vector as a skeleton, and the nucleic acid molecule in (1) is arranged between SmaI site and XbaI site.

[0054] The present application does not have special limitations on the source and type of the microorganism in (4), including but not limited to Escherichia coli, Agrobacterium and other conventional microorganisms that can carry the recombinant expression vector to achieve the purpose of the present application.

[0055] Preferably, the microorganism in (4) is Agrobacterium.

[0056] More preferably, the Agrobacterium is Agrobacterium tumefaciens.

[0057] Further preferably, the Agrobacterium tumefaciens is LBA4404 strain.

[0058] Application of any of the products in promoting the synthesis of sandalwood essential oil.

[0059] Preferably, the sandalwood essential oil is sandalwood essential oil of Santalum album L.

[0060] Application of any of the products in promoting the synthesis of santalene and / or santalol.

[0061] Preferably, the sandalwood essential oil is sandalene and / or santalol of Santalum album L.

[0062] More preferably, the santalene comprises alpha-santalene and beta-santalene.

[0063] More preferably, the santalol comprises alpha-santalol and beta-santalol.

[0064] A method for promoting the synthesis of sandalwood essential oil, increasing the expression amount of sandalwood SaJA2L protein and / or SaJA2L gene in sandalwood.

[0065] Preferably, the sandalwood is treated with any of the biological materials or any of the products for increasing the expression amount of sandalwood SaJA2L protein and / or SaJA2L gene in sandalwood.

[0066] Preferably, the promotion of the synthesis of sandalwood essential oil comprises promotion of the synthesis of santalene and / or santalol.

[0067] More preferably, the santalene comprises alpha-santalene and beta-santalene.

[0068] More preferably, the santalol comprises alpha-santalol and beta-santalol.

[0069] Preferably, the sandalwood is Santalum album L.

[0070] Compared with the prior art, the present application has the following beneficial effects:

[0071] The present application first obtains the SaJA2L gene in sandalwood, and discloses the regulation effect of the gene in the synthesis of natural sandalwood essential oil. Overexpression of the SaJA2L gene in sandalwood can significantly promote the synthesis of sandalwood essential oil. The present application provides a new technical choice for increasing the yield of natural sandalwood essential oil, and has important popularization and application value in cultivating new varieties of sandalwood with high essential oil synthesis efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0072] Figure 1This is the result of PCR amplification and electrophoresis detection of the CDS region of the SaJA2L gene (SEQ ID NO. 1) in Example 1. M represents DNA Marker, and 1 to 3 represent PCR products, respectively.

[0073] Figure 2 The electrophoresis detection results of the monoclonal bacterial solution PCR in Example 1 are shown. M represents DNA Marker, and 1 to 3 represent three monoclonal bacterial solutions, respectively.

[0074] Figure 3 This is a map of the sandalwood SaJA2L overexpression vector in Example 1.

[0075] Figure 4 These are microscopic photographs of root tissues of the WT plant and three JA2L plants in Example 2. WT represents the WT plant, and #1, #2, and #3 represent JA2L plant #1, JA2L plant #2, and JA2L plant #3, respectively.

[0076] Figure 5 The statistical results of the relative mRNA expression levels of the SaJA2L gene in the WT plant and three JA2L plants in Example 2 are shown. WT represents the WT plant, #1, #2, and #3 represent JA2L plant #1, JA2L plant #2, and JA2L plant #3, respectively. Different lowercase letters indicate significant differences among the groups (p<0.05), and the same lowercase letters indicate no significant differences among the groups (p≥0.05).

[0077] Figure 6 The statistical results of the relative mRNA expression levels of SaSSY gene and SaCYP736A167 gene in WT plants and JA2L plants in Example 2, WT represents WT plants, #2 represents JA2L plant #2, different lowercase letters indicate significant differences between groups (p < 0.05), and the same lowercase letters indicate no significant differences between groups (p ≥ 0.05).

[0078] Figure 7 The statistical results of the α-santalene content in the WT plant and three JA2L plants in Example 3 are shown. WT represents the WT plant, #1, #2, and #3 represent JA2L plant #1, JA2L plant #2, and JA2L plant #3, respectively. Different lowercase letters indicate significant differences among the groups (p<0.05), and the same lowercase letters indicate no significant differences among the groups (p≥0.05).

[0079] Figure 8The content statistics of β-amyrin in WT plants and 3 strains of JA2L plants in Example 3 are shown in Table 1, WT represents WT plants, #1, #2 and #3 represent JA2L plant #1, JA2L plant #2 and JA2L plant #3 in turn, different lowercase letters represent significant differences (p<0.05) between groups, and the same lowercase letters represent no significant difference (p≥0.05) between groups.

[0080] Figure 9 The content statistics of β-amyrin in WT plants and 3 strains of JA2L plants in Example 3 are shown in Table 1, WT represents WT plants, #1, #2 and #3 represent JA2L plant #1, JA2L plant #2 and JA2L plant #3 in turn, different lowercase letters represent significant differences (p<0.05) between groups, and the same lowercase letters represent no significant difference (p≥0.05) between groups.

[0081] Figure 10 The content statistics of β-amyrin in WT plants and 3 strains of JA2L plants in Example 3 are shown in Table 1, WT represents WT plants, #1, #2 and #3 represent JA2L plant #1, JA2L plant #2 and JA2L plant #3 in turn, different lowercase letters represent significant differences (p<0.05) between groups, and the same lowercase letters represent no significant difference (p≥0.05) between groups. DETAILED DESCRIPTION

[0082] The present application will be further described in conjunction with the accompanying drawings and specific embodiments, which are used to explain the present application and are not intended to limit the scope of the present application. In the following examples, the test methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are commercially available reagents and materials unless otherwise specified.

[0083] Example 1 Construction of Sandalwood SaJA2L Overexpression Vector

[0084] 1. Amplification of target fragment

[0085]

[0086] Genomic cDNA from root tissue of Santalum album (cultivar: Indian old mountain Santalum) was used as a template. PCR amplification was performed using the upstream cloning primer SaJA2L-F (5'-ATGGGTGTTCCGAGATCCGA-3' (SEQ ID NO. 3)) and the downstream cloning primer SaJA2L-R (5'-TCACTGTCGGGTGAACCCGA-3' (SEQ ID NO. 4)) using a gene cloning kit (Novozymes). The PCR reaction system was as follows: 2×Phanta Max Master Mix, 10 μL; ddH2O, 8 μL; SaJA2L-F (SEQ ID NO. 3), 0.5 μL; SaJA2L-R (SEQ ID NO. 4), 0.5 μL; and cDNA, 1 μL.

[0087] The PCR amplification products were collected and tested by agarose gel electrophoresis. Figure 1 As shown, the PCR amplification product has a single band and its size is consistent with the length of the CDS region of the SaJA2L gene (SEQ ID NO. 1).

[0088] 2. Carrier connection

[0089] Using double enzyme digestion and homologous recombination, the CDS region of the SaJA2L gene (SEQ ID NO. 1) amplified in the previous step was connected to the pBWA(V)HS-GFP vector (expressing green fluorescence) using restriction endonucleases SmaI and XbaI. Then, the gene was transformed into Escherichia coli and plated on LB solid medium. A single clone was picked for expansion culture, and the bacterial liquid was collected for PCR identification. Figure 2 As shown in the figure, each monoclonal clone has a single band that meets the expected size, which is a positive monoclonal clone.

[0090] The plasmid of the positive single clone was extracted and sequenced. The sequencing results showed that the sequence was correct, which means that the construct was successfully obtained. Figure 3 The sandalwood SaJA2L overexpression vector shown is denoted as pBWA(V)HS-SaJA2L vector.

[0091] Example 2 Construction of a Santalum album SaJA2L overexpression strain

[0092] 1. Construction of Agrobacterium

[0093] The pBWA(V)HS-SaJA2L vector obtained in Example 1 was introduced into Agrobacterium tumefaciens (LBA4404 strain) by freeze-thaw method to obtain Agrobacterium containing the sandalwood SaJA2L overexpression vector, which was designated as LBA4404-SaJA2L Agrobacterium.

[0094] Following the same method, the pBWA(V)HS-GFP vector was introduced into Agrobacterium tumefaciens (strain LBA4404) to obtain Agrobacterium containing an empty vector, which was designated as LBA4404-control Agrobacterium.

[0095] 2. Agrobacterium Vacuum Infiltration Transformation

[0096] (1) Preparation of reagents

[0097] Dissolve 10 mM MgCl2, 200 μM AS (acetosyringone) and 10 mM MES (2-(N-morpholino)ethanesulfonic acid) in water, mix well, adjust the pH to 5.7, and prepare the infection solution. Sterilize it by filtering with a 0.02 μm filter head and set aside.

[0098] (2) Preparation of infection solution

[0099] Agrobacterium LBA4404-SaJA2L was cultured in LB liquid medium in a constant temperature shaker at 200 rpm at 30°C until the bacterial liquid OD 600 The LBA4404-SaJA2L bacterial solution was 0.6. Take 10 mL of the LBA4404-SaJA2L bacterial solution and centrifuge at 6000 rpm for 5 minutes. Remove the supernatant and resuspend the pellet in 10 mL of infection solution. Centrifuge again at 6000 rpm for 5 minutes. Remove the supernatant and resuspend the pellet in 10 mL of infection solution to obtain the infection solution containing LBA4404-SaJA2L Agrobacterium, recorded as SaJA2L infection solution.

[0100] According to the same method, an infection solution containing LBA4404-empty Agrobacterium was prepared and recorded as the control infection solution.

[0101] (3) Conversion

[0102] Root tissue from one-year-old wild-type sandalwood plants (cultivar Santalum altissima, deposited at the South China Botanical Garden, Chinese Academy of Sciences; this biological material is publicly available from the applicant) was completely immersed in the SaJA2L infection solution and then placed in a vacuum desiccator (Tianjin Jinteng Experimental Equipment Co., Ltd., model GM-0.33A) under evacuation at 0.08 MPa for 5 minutes. Following transformation, the root tissue was cleaned with the infection solution and then cultured. The resulting sandalwood plants were designated JA2L plant #1, JA2L plant #2, and JA2L plant #3.

[0103] The root tissues of one-year-old wild-type sandalwood plants were completely immersed in the control infection solution and then transformed according to the same method. The resulting sandalwood plants were recorded as WT plants.

[0104] Laser microscope observation showed that Figure 4As shown in the figure, the root tissues of WT plant and JA2L plant #1, JA2L plant #2 and JA2L plant #3 all express green fluorescence under the irradiation of excitation light, indicating that each plant has been successfully transformed.

[0105] 3. Identification of transgenic plants

[0106] The root tissues of three JA2L plants (JA2L plant #1, JA2L plant #2 and JA2L plant #3) and WT plant were taken respectively, and RNA was extracted to obtain cDNA by reverse transcription. The cDNA was used as a template to detect the mRNA expression of SaJA2L gene, SaSSY gene and SaCYP736A167 gene by using the primers shown in Table 1 and fluorescence quantitative PCR technology.

[0107] The fluorescence quantitative PCR reaction system was as follows: 2x SYBR Green qPCR Mix, 10 μL; RNase Free ddH2O, 8 μL; cDNA, 1 μL; upstream quantitative primer (F), 0.5 μL; downstream quantitative primer (F), 0.5 μL.

[0108] Table 1. Fluorescence quantitative PCR primers

[0109]

[0110] The mRNA expression of SaJA2L gene is shown in Table 2. Figure 5 Compared with the WT plant, the mRNA expression of SaJA2L in the three JA2L plants was significantly increased, and the SaJA2L mRNA expression level of JA2L plant #2 was the highest. It is indicated that the SaJA2L overexpression strain of Santalum album is successfully constructed.

[0111] The mRNA expression of SaSSY gene and SaCYP736A167 gene is shown in Table 3. Figure 6 Compared with the WT plant, the mRNA expression of SaSSY gene and SaCYP736A167 gene in JA2L plant #2 was significantly increased. JA2L plant #1 and JA2L plant #3 also had similar detection results. It is indicated that overexpression of SaJA2L gene in Santalum album can up-regulate the terpene synthesis pathway related to the essential oil of Santalum album.

[0112] Example 3. Essential oil synthesis of Santalum album SaJA2L overexpression strain

[0113] 1. Determination of essential oil of Santalum album

[0114] Root tissues of the one-month-old WT plant and three JA2L plants (JA2L plant #1, JA2L plant #2, and JA2L plant #3) obtained in Example 2 were collected, dried, and then ground to obtain sandalwood root powder of each plant.

[0115] 0.1 g of sandalwood root powder from each plant was taken and used with 1 mL of n-hexane to extract metabolites by repeatedly shaking the extract for 3 days. The extract was collected and centrifuged at 2000 r / min for 5 min. The supernatant was taken and evaporated to dryness with nitrogen.

[0116] The dried residues of each plant were collected and dissolved in 50 μL of n-hexane. Dodecane (0.1 mg / mL) was added as an internal standard and detected according to the following method: a GC-2010 gas chromatograph (Shimadzu, Suzhou, China) was operated in electron ionization selected ion monitoring mode. The sandalwood essential oil samples were analyzed on a DB Wax fused silica column (Agilent Technologies) (30 m long, 250 μm inner diameter, 0.25 μm film thickness). The injector was operated in pulsed splitless mode, and the injector temperature was maintained at 250°C. Helium was used as the carrier gas with a flow rate of 0.8 mL / min and a pulse pressure of 25 psi for 0.5 min. The scan range was m / z 40-500. The SIM analysis was performed at m / z 93, 94, 105, 107, 119, 122, and 202 with a dwell time of 50 ms. The oven program consisted of: 50°C for 3 minutes; ramping to 250°C at 5°C / min, holding for 2 minutes; then ramping to 300°C at 5°C / min, holding for 15 minutes. Data acquisition and processing were performed using ChemStation software (Agilent Technologies). Compounds were identified by comparing mass spectra with the NIST / EPA / NIH Mass Spectral Library, version 2.0 (http: / / chemdata.nist.gov / ). The relative abundance of essential oil components was calculated by manual integration of peak areas and normalization using internal standards and the amount of tissue used (dry weight). Analysis was performed using three biological replicates, each containing two technical replicates.

[0117] 2. Measurement results

[0118] like Figures 7-10 As shown in the figure, compared with the WT plants, the contents of α-santalene, β-santalene, β-santalol, and α-santalol in the three JA2L plants were significantly increased, indicating that overexpression of the SaJA2L gene in sandalwood can promote the synthesis of sandalwood essential oil.

[0119] Finally, it should be noted that the above embodiments are intended only to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Those skilled in the art will readily appreciate that other variations or modifications may be made based on the above descriptions and concepts. It is not necessary and impossible to provide an exhaustive list of all possible implementations. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the claims of the present invention.

Claims

1. Sandalwood SaJA2L protein, characterized in that Its amino acid sequence is shown in SEQ ID NO.

2.

2. The use of sandalwood SaJA2L protein and / or SaJA2L gene in promoting the synthesis of sandalwood essential oil, characterized in that: The amino acid sequence of the sandalwood SaJA2L protein is shown in SEQ ID NO.

2.

3. Use of sandalwood SaJA2L protein and / or SaJA2L gene in promoting the synthesis of santalene and / or santalol, characterized in that: The amino acid sequence of the sandalwood SaJA2L protein is shown in SEQ ID NO.

2.

4. A biomaterial for promoting the synthesis of sandalwood essential oil, characterized in that: It is any one of the following (1) to (4): (1) A nucleic acid molecule encoding the sandalwood SaJA2L protein according to claim 1; (2) an expression cassette containing the nucleic acid molecule described in (1); (3) a recombinant expression vector containing the nucleic acid molecule described in (1); (4) A microorganism containing the recombinant expression vector described in (3).

5. The biomaterial according to claim 4, characterized in that The nucleotide sequence of the nucleic acid molecule described in (1) is shown in SEQ ID NO.

1.

6. The biomaterial according to claim 4, characterized in that The microorganism described in (4) is Agrobacterium.

7. Use of the biomaterial according to any one of claims 4 to 6 in promoting the synthesis of sandalwood essential oil.

8. A product for promoting the synthesis of sandalwood essential oil, characterized in that: The biomaterial according to any one of claims 4 to 6.

9. A method for promoting the synthesis of sandalwood essential oil, characterized in that: The expression level of the sandalwood SaJA2L protein and / or SaJA2L gene in sandalwood is increased, wherein the amino acid sequence of the sandalwood SaJA2L protein is shown in SEQ ID NO.

2.

10. The method according to claim 9, characterized in that Sandalwood is treated with the biomaterial according to any one of claims 4 to 6.

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