Pet urinary health nutritional supplement and preparation method thereof

Abstract

The invention discloses a pet urinary health nutritional supplement and a preparation method thereof, belongs to the technical field of pet food additives, provides the preparation method of the pet urinary health nutritional supplement, and provides different dosage forms of the pet urinary health nutritional supplement. The raw materials rich in anthocyanin are embedded by adopting an embedding technology, so that the oxidation speed of the anthocyanin is delayed, the anthocyanin is not easy to inactivate, and effective components are better protected; through embedding treatment, anthocyanin is in full contact with cyclodextrin, the proportion of anthocyanin fully entering a hydrophobic cavity of cyclodextrin is increased, a stable inclusion compound is formed, and the content of anthocyanin is higher; by compounding various functional raw materials, urine can be acidified, crystal generation can be prevented, mucosa can be repaired, the immunity of pets can be adjusted, the supplement is diversified in function, a user does not need to add supplements with different functions for multiple times, and the supplement provided by the invention is free of bitter taste and good in palatability.

Description

Technical Field

[0001] The present application belongs to the technical field of pet food additives, and in particular relates to a pet urinary health nutritional supplement and a preparation method thereof. Background Art

[0002] The intestines are an important part of nutrient digestion and absorption for dogs and cats, and their health plays a decisive role in the overall health of the pet. Urinary system problems in dogs and cats include urinary tract infections, bladder stones, and urethral obstruction. These problems are often caused by a variety of factors. The main causes include: insufficient water intake leading to concentrated urine, an unbalanced diet due to high magnesium, high phosphorus, high protein, and low dietary fiber, bacterial infections such as E. coli and Staphylococcus, and decreased immunity in dogs and cats. Currently, the pet urinary health products on the market have single efficacy. Most of them only focus on antibacterial or acidifying urine, lacking the synergistic effects of crystallization prevention, mucosal repair, and immune regulation. In addition, the plant extracts have a distinct bitter taste and poor palatability. Summary of the Invention

[0003] The purpose of the present application is to provide a pet urinary health nutritional supplement and a preparation method thereof, so as to solve the technical problem that pet nutritional supplements in the existing market have a single function, only have therapeutic functions, and have no preventive and protective functions.

[0004] To achieve the above objectives, the technical solution adopted in this application is to provide a method for preparing a pet urinary health nutritional supplement, which specifically comprises the following steps: (1) adding β-cyclodextrin to cranberry powder, blueberry powder and strawberry powder for encapsulation to obtain encapsulated cranberry powder, encapsulated blueberry powder and encapsulated strawberry powder, respectively; (2) Mixing perilla seed powder, dandelion powder, corn silk powder, turmeric powder, sodium hyaluronate, vitamin A, and vitamin E at a low speed to obtain mixture A; mixing inulin, mannan oligosaccharide, embedded cranberry powder, embedded blueberry powder, and embedded strawberry powder at a low speed to obtain mixture B; adding lactose-free goat milk powder, bovine colostrum powder, purple sweet potato powder, psyllium powder, and hawthorn powder into a three-dimensional motion mixer and mixing at a low speed to obtain mixture C; (3) Mixing the mixture A and the mixture B, adding them to the mixture C and mixing them at a low speed to prepare a pet urinary health nutritional supplement.

[0005] In one embodiment, The specific steps of embedding treatment are: S1. Preparation of saturated aqueous solution: Add deionized water to a container, heat, add β-cyclodextrin, and stir until completely dissolved to form a saturated aqueous solution; S2, dissolving cranberry powder, blueberry powder, and strawberry powder in ethanol respectively to prepare anthocyanin solutions; S3. Slowly add the anthocyanin solution dropwise to the saturated aqueous solution while stirring continuously; S4. After the reaction is completed, the solution is cooled to room temperature to precipitate crystals of embedded cranberry powder, embedded blueberry powder, and embedded strawberry powder respectively; the crystals are filtered, washed, and dried to obtain embedded cranberry powder, embedded blueberry powder, and embedded strawberry powder.

[0006] In one embodiment, The heating temperature in step S1 is 50° C., which can promote the binding of β-cyclodextrin to the hydrophobic cavity of anthocyanin while avoiding the destruction of heat-sensitive components by high temperature.

[0007] In one embodiment, The stirring time in step S3 is 1-3 h.

[0008] In one embodiment, The washing solvent in step S4 is cold deionized water or ethanol.

[0009] In one embodiment, In step S4, an ice bath is used for precipitation of crystals.

[0010] In one embodiment, The mass ratio of cranberry powder, blueberry powder, strawberry powder and β-cyclodextrin is 6:1:1:16.

[0011] In one embodiment, The low speed mixing was 450 rpm.

[0012] The present application also provides a dosage form of a pet urinary health nutritional supplement, which is a pet urinary health nutritional supplement prepared according to the preparation method of any of the above embodiments, prepared in the form of a powder, capsule or tablet.

[0013] The present application also provides a pet urinary health nutritional supplement, which includes, by weight, 400-420 parts of zero-lactose goat milk powder, 300-320 parts of inulin, 45-50 parts of bovine colostrum powder, 55-60 parts of cranberry powder, 20-25 parts of mannan oligosaccharides, 15-20 parts of purple sweet potato powder, 15-20 parts of plantain seed powder, 15-20 parts of hawthorn powder, 10-15 parts of dandelion powder, 8-10 parts of blueberry powder, 8-10 parts of strawberry powder, 8-10 parts of perilla seed powder, 8-10 parts of corn silk powder, 8-10 parts of turmeric powder, 3-5 parts of sodium hyaluronate, 3-5 parts of vitamin A, and 3-5 parts of vitamin E.

[0014] The present application provides a pet urinary health nutritional supplement and a preparation method thereof. The lactose-free goat milk powder, as a high-quality protein source, provides whey protein and casein with high digestibility, avoiding diarrhea caused by lactose intolerance; the natural lactoferrin and lactoperoxidase in goat milk have antibacterial and anti-inflammatory properties, which can inhibit the colonization of urinary tract pathogens (such as Escherichia coli) and indirectly reduce the risk of urinary tract infections.

[0015] Inulin is a long-chain prebiotic that selectively proliferates beneficial bacteria such as Bifidobacterium and inhibits the intestinal source of urinary tract pathogens; the short-chain fatty acids (SCFAs) produced by its fermentation can lower the pH of urine and inhibit the formation of magnesium ammonium phosphate stones; inulin combines with calcium ions in the intestine to form a soluble complex that can reduce urinary calcium excretion.

[0016] Bovine colostrum powder provides high concentrations of immunoglobulins (especially IgG and IgA), which neutralize toxins from urinary pathogens, such as the P-type ciliary antigen of Escherichia coli, through passive immunity. Its lactoferrin chelates free iron, blocking the iron supply required for bacterial growth. Proline-rich polypeptides (PRPs) in bovine colostrum can downregulate the expression of proinflammatory cytokines and alleviate bladder inflammation.

[0017] Cranberry powder is rich in proanthocyanidins (PACs), which can change the charge distribution on the surface of E. coli, preventing it from adhering to the bladder wall and causing it to be flushed out with urine.

[0018] Mannan oligosaccharides (MOS) can prevent urinary pathogens from colonizing the urothelium by competitively binding to the mannose receptors of urinary pathogens (such as Escherichia coli Type-1 fimbriae). When used synergistically with cranberry PACs, it can improve bacterial clearance.

[0019] Purple sweet potato powder provides anthocyanins and dietary fiber. Its purple color comes from its high concentration of antioxidants, which can reduce oxidative DNA damage in bladder epithelial cells. Purple sweet potato mucin can coat urethral crystals, preventing them from forming stones. Blueberry powder provides anthocyanins such as delphinidin-3-glucoside, whose antioxidant capacity (ORAC value ≥ 5000) can neutralize free radicals in urine and protect the bladder mucosa from oxidative damage. Strawberry powder is rich in ellagic acid, which can block the conversion of nitrites into carcinogenic nitrosamines, reducing the risk of bladder cancer.

[0020] Plantain seed powder is rich in soluble fiber (glucomannan), which expands into a gel upon absorbing water and absorbs ions such as oxalate and phosphate in urine. Hawthorn powder contains hyperoside and protocatechuic acid, which enhance bladder detrusor contractility (by activating M3 cholinergic receptors) and improve symptoms of weak urination. Dandelion powder contains taraxasterols and chicoric acid, which promote toxin metabolism by activating P450 enzymes in hepatocytes, thereby reducing the renal filtration load. Perilla seed powder contains α-linolenic acid (ALA, ≥50% omega-3), which exerts anti-inflammatory effects through metabolism into EPA / DHA. Its luteolin can inhibit the NF-κB pathway and reduce inflammatory bladder infiltration. Corn silk powder contains corn silk polysaccharides and saponins, which reduce calcium reabsorption by inhibiting TRPV5 channels in renal tubular epithelial cells, thereby lowering urinary calcium concentrations. Turmeric powder downregulates the NLRP3 inflammasome, thereby blocking the oxidative stress chain reaction that contributes to cystine stone formation.

[0021] Sodium hyaluronate, as a glycosaminoglycan (GAG) analogue, repairs the negatively charged protective layer on the surface of the bladder mucosa and prevents crystal adhesion.

[0022] Vitamin A maintains the differentiation and integrity of urothelial cells, and its metabolite, retinoic acid, regulates Claudin-2 protein expression and reduces urinary electrolyte reverse leakage. Vitamin E, a fat-soluble antioxidant, protects polyunsaturated fatty acids (such as DHA) on urinary system cell membranes from free radical attack. DETAILED DESCRIPTION

[0023] In order to make the technical problems, technical solutions and beneficial effects to be solved by this application more clear and understandable, this application is further described in detail. It should be understood that the specific embodiments described herein are only used to explain this application and are not used to limit this application.

[0024] Example 1 (1) Weigh 42 g lactose-free goat milk powder, 32 g inulin, 5 g bovine colostrum powder, 6 g cranberry powder, 2.5 g mannan oligosaccharide, 2 g purple sweet potato powder, 2 g psyllium powder, 1.5 g hawthorn powder, 1.5 g dandelion powder, 1 g blueberry powder, 1 g strawberry powder, 1 g perilla seed powder, 1 g corn silk powder, 1 g turmeric powder, 0.5 g sodium hyaluronate, 0.5 g vitamin A, and 0.5 g vitamin E. (2) Add β-cyclodextrin to cranberry powder, blueberry powder, and strawberry powder for encapsulation to obtain encapsulated cranberry powder, encapsulated blueberry powder, and encapsulated strawberry powder, respectively. The specific steps are as follows: S1. Preparation of saturated aqueous solution: Add 400 ml of deionized water to a container, heat to 50°C, add 16 g of β-cyclodextrin, and stir until completely dissolved to form a saturated aqueous solution; S2, dissolving 6 g cranberry powder, 1 g blueberry powder, and 1 g strawberry powder in ethanol to prepare an anthocyanin solution; S3. Slowly add the anthocyanin solution dropwise to the saturated aqueous solution and continue stirring for 3 h; S4. After the reaction is completed, the solution is cooled to room temperature, and an ice bath is used to precipitate crystals of the embedded cranberry powder, embedded blueberry powder, and embedded strawberry powder; the crystals are filtered, washed with cold deionized water, and dried to obtain the embedded cranberry powder, embedded blueberry powder, and embedded strawberry powder; (3) Mix perilla seed powder, dandelion powder, corn silk powder, turmeric powder, sodium hyaluronate, vitamin A, and vitamin E at a low speed to obtain mixture A; mix inulin, mannan oligosaccharide, embedded cranberry powder, embedded blueberry powder, and embedded strawberry powder at a low speed to obtain mixture B; put lactose-free goat milk powder, bovine colostrum powder, purple sweet potato powder, psyllium powder, and hawthorn powder into a three-dimensional motion mixer and mix at a low speed to obtain mixture C; (4) Mixing the mixture A and the mixture B, adding them to the mixture C and mixing them at a low speed of 450 rpm to obtain a pet urinary health nutritional supplement, and preparing the pet urinary health nutritional supplement into a powder.

[0025] Example 2 This embodiment differs from Example 1 in that 40 g of lactose-free goat milk powder, 30 g of inulin, 4.5 g of bovine colostrum powder, 5.5 g of cranberry powder, 2.0 g of mannan oligosaccharide, 1.5 g of purple sweet potato powder, 1.5 g of plantain seed powder, 2.0 g of hawthorn powder, 1.0 g of dandelion powder, 0.8 g of blueberry powder, 0.8 g of strawberry powder, 0.8 g of perilla seed powder, 0.8 g of corn silk powder, 0.8 g of turmeric powder, 0.3 g of sodium hyaluronate, 0.3 g of vitamin A, and 0.3 g of vitamin E were weighed, and the remaining operations were the same to prepare a powder.

[0026] Example 3 This embodiment differs from embodiment 1 in that in step (2) S4, the washing solvent is ethanol, and the remaining operations are the same to obtain a powder.

[0027] Example 4 This embodiment differs from Example 1 in that the pet urinary health nutritional supplement is prepared into capsules, and the remaining operations are the same.

[0028] Example 5 This embodiment differs from Example 1 in that the pet urinary health nutritional supplement is prepared into tablets, and the remaining operations are the same.

[0029] Experimental example 24 healthy, similar-weight, and mentally healthy adult cats, including 12 males and 12 females, were selected, and the breeds were British shorthair, American shorthair, and Ragdoll cats. The cats were randomly divided into two groups, half male and half female. The blank group was fed only basal cat food, and the experimental group was fed 5 g of the powder prepared in Example 1 daily on the basis of the basal cat food, mixed with the basal food and fed. The experiment lasted for 42 days. The cat drinking fountain was filled with water at 8 o'clock every morning, and the remaining water volume was recorded before adding water the next day. The difference was the water intake. The water intake was recorded daily, and the average weekly water intake was calculated for subsequent comparison of changes in water intake. Urine of the experimental cats was collected for three consecutive days each week for measuring urine pH. The results showed that compared with the blank group, the average weekly water intake of the experimental cats in the experimental group was significantly increased, and the urine pH tended to decrease.

[0030]

[0031] The present application provides a preparation method of a urinary health nutritional supplement for pets, which specifically comprises the following steps: adding β-cyclodextrin to cranberry powder, blueberry powder and strawberry powder for embedding treatment to obtain an inclusion compound; mixing perilla seed powder, dandelion powder, corn silk powder, turmeric powder, sodium hyaluronate, vitamin A and vitamin E to obtain a mixture A; mixing inulin, mannan oligosaccharide and the inclusion compound to obtain a mixture B; mixing zero lactose goat milk powder, bovine colostrum powder, purple sweet potato powder, psyllium powder and hawthorn powder to obtain a mixture C; mixing the mixtures A and B and adding them to the mixture C for mixing to prepare a urinary health nutritional supplement for pets; and providing a method for preparing a urinary health nutritional supplement for pets. Different dosage forms of urinary health nutritional supplements for pets; the application adopts encapsulation technology to encapsulate raw materials rich in anthocyanins, which slows down the oxidation rate of anthocyanins, makes them less likely to be inactivated, and better protects the effective ingredients; the encapsulation treatment allows anthocyanins to fully contact with cyclodextrins, increases the proportion of anthocyanins fully entering the hydrophobic cavity of cyclodextrins, forms a stable inclusion complex, and has a higher anthocyanin content; through the compounding of multiple functional raw materials, it can not only acidify urine, but also prevent the formation of crystals, repair mucous membranes, and regulate the immunity of pets, making the supplements multifunctional, and users do not need to add supplements with different functions multiple times, and the supplements provided by the application have no bitterness and good palatability.

[0032] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. Throughout the description of this application, "plurality" means two or more, unless otherwise specifically defined.

[0033] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present application should be included in the scope of protection of the present application.

Claims

1. A method for preparing a pet urinary health nutritional supplement, characterized in that: The specific steps include: (1) adding β-cyclodextrin to cranberry powder, blueberry powder and strawberry powder for encapsulation to obtain encapsulated cranberry powder, encapsulated blueberry powder and encapsulated strawberry powder; (2) Mix perilla seed powder, dandelion powder, corn silk powder, turmeric powder, sodium hyaluronate, vitamin A, and vitamin E to obtain mixture A; mix inulin, mannan oligosaccharide, the embedded cranberry powder, the embedded blueberry powder, and the embedded strawberry powder to obtain mixture B; mix lactose-free goat milk powder, bovine colostrum powder, purple sweet potato powder, psyllium powder, and hawthorn powder to obtain mixture C; (3) Mixing the mixture A and the mixture B, and adding the mixture to the mixture C to prepare a pet urinary health nutritional supplement.

2. The method for preparing a pet urinary health nutritional supplement according to claim 1, characterized in that: The specific steps of the embedding process are: S1. Preparation of saturated aqueous solution: Add deionized water to a container, heat, add β-cyclodextrin, and stir until completely dissolved to form a saturated aqueous solution; S2, dissolving cranberry powder, blueberry powder, and strawberry powder in ethanol respectively to prepare anthocyanin solutions; S3, slowly adding the anthocyanin solution dropwise to the saturated aqueous solution while continuously stirring; S4. After the reaction is completed, the solution is cooled to room temperature to precipitate crystals of embedded cranberry powder, embedded blueberry powder, and embedded strawberry powder respectively; the crystals are filtered, washed, and dried to obtain embedded cranberry powder, embedded blueberry powder, and embedded strawberry powder.

3. The method for preparing a pet urinary health nutritional supplement according to claim 2, characterized in that: The heating temperature in step S1 is 50°C.

4. The method for preparing a pet urinary health nutritional supplement according to claim 2, characterized in that: The stirring time in step S3 is 1-3 h.

5. The method for preparing a pet urinary health nutritional supplement according to claim 2, characterized in that: The washing solvent in step S4 is cold deionized water or ethanol.

6. The method for preparing a pet urinary health nutritional supplement according to claim 2, characterized in that: In step S4, an ice bath is used for precipitation of crystals.

7. The method for preparing a pet urinary health nutritional supplement according to claim 1, characterized in that: The mass ratio of the cranberry powder, blueberry powder, strawberry powder and β-cyclodextrin is 6:1:1:

16.

8. The method for preparing a pet urinary health nutritional supplement according to claim 1, characterized in that: The mixing speed was 450 rpm.

9. A dosage form of a pet urinary health nutritional supplement, characterized in that: The pet urinary health nutritional supplement prepared by the preparation method according to any one of claims 1 to 8 is prepared into a powder, capsule or tablet.

10. A nutritional supplement for pet urinary health, characterized in that: Calculated by weight, it includes 400-420 parts of zero-lactose goat milk powder, 300-320 parts of inulin, 45-50 parts of bovine colostrum powder, 55-60 parts of embedded cranberry powder, 20-25 parts of mannan oligosaccharide, 15-20 parts of purple sweet potato powder, 15-20 parts of plantain seed powder, 15-20 parts of hawthorn powder, 10-15 parts of dandelion powder, 8-10 parts of embedded blueberry powder, 8-10 parts of embedded strawberry powder, 8-10 parts of perilla seed powder, 8-10 parts of corn silk powder, 8-10 parts of turmeric powder, 3-5 parts of sodium hyaluronate, 3-5 parts of vitamin A, and 3-5 parts of vitamin E.

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