Human adipose tissue source matrix membrane preparation device

Abstract

The invention provides a preparation device for a human adipose tissue-derived matrix diaphragm. The preparation device comprises a lifting bracket, according to the grinding and rubbing driving unit, a driving circular plate is eccentrically connected with the output end of a rotary driving part; a plurality of guide circular holes are formed in the plate surface of the movable plate in the circumferential direction, and a notch embedded with the outer edge of the driving circular plate is formed in the middle of the movable plate; the rotating plate is arranged at the bottom of the movable plate, and a plurality of guide rods penetrating through the guide circular holes are fixed to the top of the rotating plate; the guide rods are connected with the bottom of the annular driving part and drive the annular driving part to rotate along a circle; the grinding and rubbing execution unit comprises a crushing rod and a plurality of grinding and rubbing rods; the plurality of grinding and rubbing rods are fixed at the bottom of the movable plate and are uniformly arranged along the circumferential direction; the liquid inlet unit is communicated with the cover cylinder and is used for supplying liquid; the operation cylinder is arranged below the cover cylinder; a metal net cylinder is arranged in the operation cylinder; and the liquid discharging unit is communicated with the operation cylinder and pumps liquid. The device can replace manual work to quickly remove cell components and oil drops of abdominal wall repair and excision adipose tissues so as to facilitate formation of membranes with uniform thickness.

Description

Technical Field

[0001] The present invention relates to the technical field of membrane preparation instruments, and in particular to a device for preparing a human adipose tissue-derived matrix membrane. Background Art

[0002] The extracellular matrix (ECM) is an important structural component of connective tissue. The ECM secreted by different cells constitutes the microenvironment of the cells, and the ECM provides a substrate for cell tissue to attach, and is also the key to tissue regeneration and tissue repair. Tissue-based ECM biomaterials and devices have various medical applications, such as heart valves, porcine SIS, human skin and bovine pericardium. Therefore, biomembrane materials based on tissue matrix have great potential for future medical applications. However, the raw materials currently used to prepare biomembranes are mostly from heterologous sources such as pigs and cattle, which pose a certain risk of immune rejection. In addition, they are different from tissue components of human origin and are easily degraded, resulting in poor performance. The lack of human raw materials has led to problems with preparation costs and large-scale production. How to solve the above problems has become the key.

[0003] Human adipose decellularized extracellular matrix is ​​rich in various collagen components of the natural adipose tissue extracellular matrix, growth factors and other tissue regeneration-related bioactive factors, as well as active ingredients such as fibronectin and elastin. It can provide a good microenvironment for cells, regulate cell behavior, and induce adipogenesis for a long time. It is a material with a wide range of human sources and good biological activity, and can be used to make biological membrane materials based on tissue matrix.

[0004] When preparing human adipose tissue-derived matrix membrane sheets, it is necessary to mechanically crush fat fibers, repeatedly clean them to remove cell debris and oil droplets, and then press them into a membrane state. However, conventional cleaning of fat fibers after crushing is done by manually pressing and rubbing the fat fibers with a metal mesh to further break up the mature fat, and timely flushing is required to achieve optimal removal. However, this manual operation is relatively cumbersome and inefficient, and is easily mixed with foreign matter. The purity of the fat fibers is not high, and there is an urgent need for an automated device to replace manual crushing and cleaning. Summary of the Invention

[0005] To achieve the technical goal of replacing manual rapid removal of cellular components and oil droplets from adipose tissue removed during abdominal wall repair and thereby assisting in forming a membrane sheet of uniform thickness, the present invention provides a device for preparing a human adipose tissue-derived matrix membrane sheet. The present invention provides the following technical solutions.

[0006] A device for preparing a human adipose tissue-derived matrix membrane sheet, comprising:

[0007] Lifting bracket;

[0008] The grinding drive unit includes a driving circular plate, a movable plate, a rotating plate, and an annular driving member mounted on the lifting bracket, a rotating driving member, and a cover cylinder; the driving circular plate is eccentrically connected to the output end of the rotating driving member; the movable plate surface is provided with a plurality of guide circular holes along the circumference, and a notch is provided in the middle thereof to be embedded in the outer edge of the driving circular plate; the rotating plate is arranged at the bottom of the movable plate, and a plurality of guide rods are fixed to the top thereof, which respectively pass through the plurality of guide circular holes; the guide rods are all connected to the bottom of the annular driving member to drive the circular rotation;

[0009] The grinding execution unit includes a breaker rod and multiple grinding rods; one end of the breaker rod is arranged at the bottom of the rotating plate; the multiple grinding rods are fixed to the bottom of the movable plate and are evenly arranged along the circumference; wherein the rotating drive member drives the driving circular plate and then drives the movable plate to eccentrically rotate; the annular drive member drives the rotating plate to rotate in the opposite direction, and the guide rod drives the movable plate and the driving circular plate to rotate in the opposite direction relative to each other, forming an eccentric rotation motion;

[0010] a liquid inlet unit, arranged on the lifting bracket, connected to the cover cylinder and supplying liquid;

[0011] The working cylinder is arranged below the cover cylinder; a metal mesh cylinder is built into the working cylinder; wherein the plurality of grinding rods are driven by the movable plate to circulate and rotate on the inner wall of the metal mesh cylinder;

[0012] The liquid discharge unit is arranged at the bottom of the lifting bracket, is connected with the working cylinder and draws liquid.

[0013] Preferably, the lifting bracket includes:

[0014] Base; the operating cylinder is installed on the top of the base;

[0015] A rotating frame is rotatably arranged on the top of the base; the rotating frame has a sliding groove;

[0016] An operating panel, the center of which is slidably disposed in the sliding groove of the rotating frame;

[0017] A lead screw passes through the operating plate and is threadedly engaged with the operating plate; two ends of the lead screw are rotatably connected to two ends of the sliding groove respectively;

[0018] The first motor is fixedly arranged on the top of the rotating frame, and the output shaft of the first motor is transmission-connected with the lead screw.

[0019] Preferably, the annular drive member comprises:

[0020] An L-shaped rack rod is mounted on the top of one end of the operating panel;

[0021] The second motor is fixedly arranged on the top of the L-shaped frame rod, and the output shaft of the second motor is fixedly connected to the driving disc.

[0022] Preferably, the rotary drive member comprises:

[0023] An arc-shaped guide plate is fixedly arranged at the bottom of the L-shaped frame rod; the lower end of the arc-shaped guide plate is an arc-shaped T-shaped guide strip;

[0024] A gear ring, the top of which is provided with an annular groove that cooperates with the T-shaped guide strip; a plurality of guide rods are fixedly connected to the bottom of the gear ring;

[0025] The third motor is fixedly arranged on the top of the L-shaped frame rod, and its output shaft passes through the gear ring and is fixedly connected to the driving gear; the driving gear is meshed with the gear ring.

[0026] Preferably, the base has a built-in fourth motor, and the output shaft of the fourth motor is fixedly connected to the lower end of the rotating frame.

[0027] Preferably, it further comprises a film forming component; the film forming component comprises:

[0028] The first screw slide; the operating plate has a first extension plate facing the other direction, which is inverted and fixedly arranged at the bottom of the first extension plate;

[0029] An L-shaped arm, one end of which is mounted on the bottom of the first lead screw slide and is driven to move by the first lead screw slide;

[0030] The sampling device is mounted on the L-shaped arm and includes a plurality of transfer tubes arranged side by side, and an air extraction micro pump and an air charging micro pump respectively connected to the transfer tubes; the plurality of transfer tubes are driven by a fourth motor and a lifting bracket to move into the working cylinder for sampling;

[0031] A plurality of pressing platforms are evenly and linearly arranged along the moving direction of the first lead screw slide; each of the pressing platforms is provided with a plurality of drainage holes;

[0032] The second screw slide and the third screw slide are respectively mounted on both sides of the pressing platform;

[0033] A movable platform is mounted on the third screw slide; a support plate is mounted on the top of the movable platform;

[0034] A pressing groove, the back of which is connected to the support plate via a pressing hydraulic cylinder; the groove body of the pressing groove matches the outer contour of the pressing platform;

[0035] Two placement grooves are respectively provided on the outer sides of the pressing platform at both ends; filter paper and mesh are respectively placed in the two placement grooves;

[0036] The paper picking assembly is mounted on the second lead screw slide, is used for picking up and placing filter paper and mesh onto the pressing platform, and is driven to move by the second lead screw slide.

[0037] Preferably, the paper picking assembly includes:

[0038] A mounting block, the bottom of which is connected to the slide of the second screw slide via a longitudinal telescopic rod;

[0039] A limiting plate, one end of which is fixedly connected to the mounting block; two movable grooves are formed on the top of the limiting plate;

[0040] A transverse telescopic rod fixedly mounted on the top of the mounting block;

[0041] The tops of the two movable hooks are fixedly connected to the transverse telescopic rod and pass through the two movable slots respectively; two long slots that cooperate with the movable hooks are provided on both sides of the filter paper and the mesh.

[0042] Preferably, the working cylinder and the metal mesh cylinder are provided in two groups, one of which cooperates with the rubbing drive unit and the rubbing execution unit;

[0043] Also included is a hydraulic fracturing assembly; the hydraulic fracturing assembly includes:

[0044] Crushing hydraulic cylinder; the operating plate further has a second extension plate in another direction, and the end of the crushing hydraulic cylinder is fixedly arranged at the bottom of the second extension plate;

[0045] The crushing hydraulic plate is fixedly connected to the telescopic end of the crushing hydraulic cylinder and cooperates with another group of the working cylinder and the metal mesh cylinder.

[0046] Preferably, the grinding rod and the crushing rod are both equipped with a built-in drive motor; the drive motor drives the grinding rod or the crushing rod to rotate.

[0047] Beneficial effects of the present invention:

[0048] This invention proposes a device for preparing human adipose tissue-derived matrix membrane sheets. The device features two workstation modes, enabling switching between multiple steps and further enabling semi-automated membrane preparation, including adipose tissue fragmentation, oscillation cleaning, pressing and rubbing cleaning, and wrapping and pressing membrane formation, ultimately resulting in a superior membrane sheet. The device mimics the manual pressing and rubbing action, employing a centrifugal rotation drive mode to rub against the wall of a metal mesh cylinder. Combined with a liquid inlet unit, it automatically cleans adipose fibers, achieving optimal fat fragmentation and removal of cell debris and oil droplets, thereby improving membrane quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1This is a diagram of the overall assembly structure of a device for preparing a human adipose tissue-derived matrix membrane sheet according to an embodiment of the present invention;

[0050] Figure 2 This is a partial structural diagram of the friction drive unit and the friction execution unit of the apparatus for preparing a human adipose tissue-derived matrix membrane according to an embodiment of the present invention;

[0051] Figure 3 This is a front view of the friction drive unit and the friction execution unit of the device for preparing a human adipose tissue-derived matrix membrane according to an embodiment of the present invention;

[0052] Figure 4 2. It is a structural diagram of a membrane-forming component of a device for preparing a human adipose tissue-derived matrix membrane sheet according to an embodiment of the present invention;

[0053] Figure 5 This is a partial structural diagram of a paper-taking assembly of a device for preparing a human adipose tissue-derived stromal membrane sheet according to an embodiment of the present invention;

[0054] Figure 6 This is an exploded view of the filter paper and mesh of the human adipose tissue-derived matrix membrane preparation device according to an embodiment of the present invention during membrane formation;

[0055] Figure 7 This is a schematic diagram of the filter paper and mesh after forming a membrane in the apparatus for preparing a human adipose tissue-derived matrix membrane according to an embodiment of the present invention;

[0056] Figure 8 This is the process for preparing the human adipose tissue-derived matrix membrane sheet according to an embodiment of the present invention.

[0057] Among them, 1. Base; 2. Grinding rod; 3. Crushing rod; 4. Cover cylinder; 5. Movable plate; 6. Driving circular plate; 7. Second motor; 8. L-shaped frame; 9. Operation panel; 10. Lead screw; 11. Rotating frame; 12. First motor; 13. Crushing hydraulic cylinder; 14. Crushing hydraulic plate; 15. Limiting plate; 16. Transfer pipe; 17. Moving platform; 18. Liquid inlet unit; 19. Rotating plate; 20. Metal mesh cylinder; 21. Working cylinder; 22. Liquid discharge unit; 23. The third motor; 24. Drive gear; 25. Arc guide plate; 26. Gear ring; 27. Guide rod; 28. Pressing groove; 29. ​​Placement groove; 30. Second lead screw slide; 31. First lead screw slide; 32. Third lead screw slide; 33. L-shaped arm; 34. Support plate; 35. Pressing hydraulic cylinder; 36. Pressing platform; 37. Horizontal telescopic rod; 38. Movable hook; 39. Mounting block; 40. Longitudinal telescopic rod; 41. Filter paper; 42. Mesh; 43. Diaphragm. DETAILED DESCRIPTION

[0058] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0059] When preparing a human adipose tissue-derived matrix membrane sheet, it is necessary to obtain fat fibers by mechanical crushing, and repeatedly wash them to remove cell debris and oil droplets, and then press them into a membrane state. However, the conventional cleaning of fat fibers after crushing is to manually use a metal mesh to further press and rub the fat fibers to achieve the purpose of further crushing mature fat, and it is necessary to rinse them in time to achieve a better removal state. However, this manual operation is relatively cumbersome and inefficient, and it is very easy to mix with foreign matter. The purity of the fat fibers is not high, and an automated equipment is urgently needed to replace manual crushing and cleaning. To this end, this embodiment proposes a human adipose tissue-derived matrix membrane preparation device, such as Figure 1-5 As shown, it includes a lifting bracket, a grinding drive unit, a grinding execution unit, a liquid inlet unit 18, a liquid discharge unit 22 and a working cylinder 21.

[0060] like Figure 2-3 As shown, the friction drive unit includes a driving circular plate 6, a movable plate 5, a rotating plate 19, and an annular driving member, a rotating driving member and a cover cylinder 4 mounted on a lifting bracket; the driving circular plate 6 is eccentrically connected to the output end of the rotating driving member; the movable plate 5 is provided with a plurality of guide circular holes along the circumferential direction, and a notch is provided in the middle thereof to be embedded in the outer edge of the driving circular plate 6; the rotating plate 19 is arranged at the bottom of the movable plate 5, and a plurality of guide rods 27 are fixed on the top thereof, which pass through the plurality of guide circular holes respectively; the guide rods 27 are all connected to the bottom of the annular driving member and drive it to rotate in a circular shape; the friction execution unit includes a crushing rod 3 and a plurality of friction rods 2; one end of the crushing rod 3 is arranged at the bottom of the rotating plate 19; a plurality of The grinding rods 2 fix the bottom of the movable plate 5 and are evenly arranged along the circumferential direction; wherein, the rotating driving member drives the driving circular plate 6 and then drives the movable plate 5 to rotate eccentrically; the annular driving member drives the rotating plate 19 to rotate in the opposite direction, and through the guide rod 27, the movable plate 5 and the driving circular plate 6 are driven to rotate in the opposite direction, forming an eccentric rotational motion; the liquid inlet unit 18 is arranged on the lifting bracket, connected with the cover cylinder 4 and supplies liquid; the working cylinder 21 is arranged below the cover cylinder 4; the working cylinder 21 has a metal mesh cylinder 20 built in; wherein, multiple grinding rods 2 are driven by the movable plate 5 to circulate and rotate on the inner wall of the metal mesh cylinder 20; the liquid discharge unit 22 is arranged at the bottom of the lifting bracket, connected with the working cylinder 21 and draws liquid.

[0061] like Figure 1As shown, the lifting bracket includes a base 1, a rotating frame 11, an operating panel 9, a screw 10, and a first motor 12. The operating cylinder 21 is mounted on the top of the base 1; the rotating frame 11 is rotatably mounted on the top of the base 1; the rotating frame 11 has a sliding groove; the middle portion of the operating panel 9 is slidably mounted within the sliding groove of the rotating frame 11; the screw 10 passes through the operating panel 9 and is threadedly engaged with the operating panel 9; the two ends of the screw 10 are respectively rotatably connected to the two ends of the sliding groove; the first motor 12 is fixedly mounted on the top of the rotating frame 11, and its output shaft is in transmission connection with the screw 10. To achieve the replacement of workstations, the base 1 is equipped with a fourth motor, the output shaft of which is fixedly connected to the lower end of the rotating frame 11, adding a workstation conversion function.

[0062] like Figure 2 and Figure 3 As shown, the annular drive member includes an L-shaped frame rod 8 and a second motor 7. The L-shaped frame rod 8 is mounted on the top of one end of the operating panel 9; the second motor 7 is fixedly mounted on the top of the L-shaped frame rod 8, and its output shaft is fixedly connected to the driving disc 6. The rotary drive member includes an arc-shaped guide plate 25, a gear ring 26 and a third motor 23. The arc-shaped guide plate 25 is fixedly mounted on the bottom of the L-shaped frame rod 8; the lower end of the arc-shaped guide plate 25 is an arc-shaped T-shaped guide bar; the top of the gear ring 26 is provided with an annular groove that cooperates with the T-shaped guide bar; multiple guide rods 27 are fixedly connected to the bottom of the gear ring 26; the third motor 23 is fixedly mounted on the top of the L-shaped frame rod 8, and its output shaft passes through the gear ring 26 and is fixedly connected to the drive gear 24; the drive gear 24 is meshed with the gear ring 26.

[0063] like Figure 4-5 As shown, it also includes a film forming component; the film forming component includes a first screw slide 31, an L-shaped arm 33, a sampling device, a second screw slide 30, a third screw slide 32, a moving platform 17, a pressing groove 28, two placement grooves 29, a paper picking component and multiple pressing platforms 36.

[0064] The operating panel 9 has a first extension plate facing the other direction, inverted and fixed to its bottom. One end of an L-shaped arm 33 is mounted on the bottom of the first lead screw slide 31 and is driven by the first lead screw slide 31. The sampling device is mounted on the L-shaped arm 33 and includes multiple transfer tubes 16 arranged side by side, and micro-pumps for suction and inflation, respectively, connected to the transfer tubes 16. The multiple transfer tubes 16 are driven by a fourth motor and a lifting bracket to move into the working cylinder 21 for sampling. Multiple pressing platforms 36 are evenly and linearly arranged along the moving direction of the first screw slide 31; each pressing platform 36 is provided with multiple drainage holes; the second screw slide 30 and the third screw slide 32 are respectively mounted on both sides of the pressing platform 36, wherein the movable platform 17 is mounted on the third screw slide 32; a support plate 34 is mounted on the top of the movable platform 17; the back of the pressing groove 28 is connected to the support plate 34 through a pressing hydraulic cylinder 35; the groove body of the pressing groove 28 is matched with the outer contour of the pressing platform 36; two placement grooves 29 are respectively arranged on the outside of the pressing platform 36 at both ends; filter paper 41 and mesh 42 are respectively placed in the two placement grooves 29; the paper picking assembly is mounted on the second screw slide 30, for taking and placing filter paper 41 and mesh 42 onto the pressing platform 36, and is driven to move by the second screw slide 30.

[0065] like Figure 5 As shown, the paper picking assembly includes a mounting block 39, a limiting plate 15, a transverse telescopic rod 37, and two movable hooks 38. The bottom of the mounting block 39 is connected to the slide of the second screw slide 30 via a longitudinal telescopic rod 40; one end of the limiting plate 15 is fixedly connected to the mounting block 39; the top of the limiting plate 15 defines two movable slots; the transverse telescopic rod 37 is fixedly mounted on the top of the mounting block 39; the tops of the two movable hooks 38 are both fixedly connected to the transverse telescopic rod 37 and extend through the two movable slots respectively; and two long slots are provided on both sides of the filter paper 41 and the mesh 42, which cooperate with the movable hooks 38.

[0066] Furthermore, in order to increase the crushing effect, e.g. Figure 1 As shown, there are two sets of working cylinders 21 and metal mesh cylinders 20, one of which cooperates with the friction drive unit and the friction execution unit. The hydraulic crushing assembly is also included, which includes a crushing hydraulic cylinder 13 and a crushing hydraulic plate 14. The operating panel 9 also has a second extension plate in the other direction, with the end of the crushing hydraulic cylinder 13 fixedly mounted on the bottom of the second extension plate. The crushing hydraulic plate 14 is fixedly connected to the telescopic end of the crushing hydraulic cylinder 13 and cooperates with the other set of working cylinders 21 and metal mesh cylinders 20.

[0067] In addition, both the grinding rod 2 and the crushing rod 3 are equipped with a drive motor; the drive motor drives the grinding rod 2 or the crushing rod 3 to rotate.

[0068] In this embodiment, Figure 8As shown in FIG, the preparation process of the human adipose tissue-derived matrix membrane preparation device specifically includes:

[0069] S1: Obtaining adipose tissue fibers for abdominal wall repair and resection. Place the adipose tissue fibers into the metal mesh cylinder 20 of a set of working cylinders 21. Drive the fourth motor to rotate, driving the lifting bracket to rotate the hydraulic crushing assembly above the metal mesh cylinder 20 of the set of working cylinders 21. Drive the lifting bracket to move the crushing hydraulic cylinder 13 and crushing hydraulic plate 14 into the metal mesh cylinder 20. Drive the crushing hydraulic cylinder 13 to extend, cooperating with the crushing hydraulic plate 14 to crush the adipose tissue fibers.

[0070] S2: Manually transfer the crushed adipose tissue fibers to the metal mesh cylinder 20 of another set of working cylinders 21. The fourth motor is driven to rotate, driving the lifting bracket to rotate, and the grinding drive unit and the grinding execution unit are rotated above the working cylinder 21. The lifting bracket is driven, and the cover cylinder 4 enters between the working cylinder 21 and the metal mesh cylinder 20. The crushing rod 3 enters the metal mesh cylinder 20 and is driven to rotate. In conjunction with the rotation of the multiple grinding rods 2, the adipose tissue is crushed to form a fat tissue paste.

[0071] S3: Drive the lifting bracket to move the rubbing execution unit out of the working cylinder 21, select and retain the larger fat fibers rich in fat matrix, and place the rubbing execution unit into the working cylinder 21 again. The liquid inlet unit 22 inputs physiological saline or deionized water, and the liquid discharge unit 22 performs the liquid extraction operation. The multiple rubbing rods 2 rotate to invert and shake the larger fat fibers to clean the residual blood and swelling fluid, and obtain the cleaned fat tissue.

[0072] S4: The rotary drive member is driven, driving the driving circular plate 6 and, in turn, the movable plate 5 in eccentric rotation. The annular drive member drives the rotating plate 19 in the opposite direction, and, through the guide rod 27, the movable plate 5 and the driving circular plate 6 in opposite directions, creating an eccentric rotational motion. Driven by the movable plate 5, the multiple grinding rods 2 circulate and roll on the inner wall of the metal mesh cylinder 20. The mesh cylinder 20 has a pore pitch of 1-2.0 mm. This process takes approximately 1-2 minutes, at which point the fat fibers appear white.

[0073] S5: The liquid inlet unit 22 inputs physiological saline or deionized water to clean the fat fibers obtained in S4, rinsing 1-3 times, each time for 2-5 minutes, and the liquid discharge unit 22 performs a liquid extraction operation.

[0074] S6: The lifting bracket is driven to move the grinding execution unit out of the working cylinder 21. The fourth motor is driven to rotate, driving the lifting bracket to rotate the first screw slide 31 above the working cylinder 21. The lifting bracket is driven to move the multiple transfer tubes 16 into the working cylinder 21. The vacuum micropump is driven to evacuate the multiple transfer tubes 16 to create negative pressure, thereby sucking the paste-like fat fibers into the multiple transfer tubes 16.

[0075] S7: Lay the filter paper 41 on the pressing table 36, wherein two long grooves cooperating with the movable hook 38 are provided on both sides of the filter paper 41 and the mesh 42; drive the second screw slide 30 to drive the paper picking assembly to move, and drive the longitudinal telescopic rod and the transverse telescopic rod (an electric telescopic rod assembly can be used) respectively to drive the movable hook 38 to hook the filter paper 41 through the two long grooves of the filter paper 41, and drive the second screw slide 30 and the transverse telescopic rod again to release the filter paper.

[0076] S8: Laying the mesh 42, the laying method is the same as S7.

[0077] S9: By driving the fourth motor, multiple transfer tubes 16 are rotated to above the pressing platform 36; by driving the first screw slide 31, the transfer tubes 16 are driven to move along the laying direction of the multiple pressing platforms 36, and at the same time, the inflation micro pump is driven to inflate the inside of the transfer tubes 16, and the paste-like fat fibers flow out from the tube mouths of the transfer tubes 16 and are evenly laid on the mesh 42 of the multiple pressing platforms 36.

[0078] S10: Lay the second mesh 42 and filter paper 41 to form an upper and lower clamping structure, such as Figure 6 The laying method is the same as S7.

[0079] S11: Drive the third screw slide 32 to drive the pressing hydraulic cylinder 35 to move to the top of the pressing platform 36, and drive the pressing hydraulic cylinder 35 to drive the pressing groove 28 to press the filter paper 41, the mesh 42 and the fat fiber; when pressing, the filter paper 41 and the mesh 42 are wrapped at the four corners, and the cross section of the diaphragm is the same as that of the pressing platform 36. The specific state is as follows Figure 7 As shown. The pressing time of the hydraulic cylinder 35 is 5-10 minutes. Multiple diaphragm pressings are completed in sequence.

[0080] S12: Remove the upper and lower filter papers 41 and the mesh 42 to obtain the prepared membrane.

[0081] This embodiment proposes a device for preparing human adipose tissue-derived matrix membrane sheets. This device features two workstation modes, enabling switching between multiple steps and further enabling semi-automated membrane preparation, including adipose tissue fragmentation, oscillation cleaning, pressing and rubbing cleaning, and wrapping and pressing membrane formation, ultimately resulting in a superior membrane sheet. This device mimics the manual pressing and rubbing motions, employing a centrifugal rotation drive mode to rub against the wall of a metal mesh cylinder. Combined with a liquid inlet unit, it automatically cleans adipose fibers, achieving optimal fat fragmentation and removal of cell debris and oil droplets, thereby improving membrane quality.

[0082] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A device for preparing a human adipose tissue-derived matrix membrane sheet, characterized in that: include: Lifting bracket; A grinding drive unit comprises a driving circular plate (6), a movable plate (5), a rotating plate (19), and an annular driving member, a rotating driving member and a cover cylinder (4) mounted on the lifting bracket; the driving circular plate (6) is eccentrically connected to the output end of the rotating driving member; the movable plate (5) is provided with a plurality of guide circular holes along the circumferential direction, and a notch is provided in the middle thereof to be embedded in the outer edge of the driving circular plate (6); the rotating plate (19) is arranged at the bottom of the movable plate (5), and a plurality of guide rods (27) respectively passing through the plurality of guide circular holes are fixed to the top of the rotating plate (19); the guide rods (27) are all connected to the bottom of the annular driving member to drive the rotating member to rotate in a circular direction; A grinding execution unit comprises a crushing rod (3) and a plurality of grinding rods (2); one end of the crushing rod (3) is arranged at the bottom of the rotating plate (19); the plurality of grinding rods (2) are fixed to the bottom of the movable plate (5) and are evenly arranged along the circumference; wherein the rotating drive member drives the driving circular plate (6) and then drives the movable plate (5) to rotate eccentrically; the annular drive member drives the rotating plate (19) to rotate in the opposite direction, and through the guide rod (27), the movable plate (5) and the driving circular plate (6) are moved to rotate in the opposite direction relative to each other, thereby forming an eccentric rotation motion; A liquid inlet unit (18) is provided on the lifting bracket and is in communication with the cover cylinder (4) to supply liquid; The working cylinder (21) is arranged below the cover cylinder (4); the working cylinder (21) has a metal mesh cylinder (20) built therein; wherein the plurality of grinding rods (2) are driven by the movable plate (5) to circulate and rotate on the inner wall of the metal mesh cylinder (20); The liquid discharge unit (22) is arranged at the bottom of the lifting bracket, is connected with the working cylinder (21) and is used to discharge liquid.

2. The human adipose tissue-derived matrix membrane preparation device according to claim 1, characterized in that: The lifting bracket includes: Base (1); the operating cylinder (21) is installed on the top of the base (1); A rotating frame (11) is rotatably arranged on the top of the base (1); the rotating frame (11) has a sliding groove; An operating panel (9), the center portion of which is slidably disposed in a sliding groove of the rotating frame (11); A lead screw (10) passes through the operating plate (9) and is threadably engaged with the operating plate (9); two ends of the lead screw (10) are rotatably connected to two ends of the sliding groove respectively; The first motor (12) is fixedly arranged on the top of the rotating frame (11), and its output shaft is transmission-connected to the lead screw (10).

3. The human adipose tissue-derived matrix membrane preparation device according to claim 2, characterized in that: The annular drive member comprises: An L-shaped rod (8) is mounted on the top of one end of the operating panel (9); The second motor (7) is fixedly arranged on the top of the L-shaped frame rod (8), and its output shaft is fixedly connected to the driving disc (6).

4. The human adipose tissue-derived matrix membrane preparation device according to claim 2, characterized in that: The rotary drive member comprises: An arc-shaped guide plate (25) is fixedly arranged at the bottom of the L-shaped frame rod (8); the lower end of the arc-shaped guide plate (25) is an arc-shaped T-shaped guide strip; The gear ring (26) has an annular groove on its top for cooperating with the T-shaped guide strip; the plurality of guide rods (27) are fixedly connected to the bottom of the gear ring (26); The third motor (23) is fixedly arranged on the top of the L-shaped frame rod (8), and its output shaft passes through the gear ring (26) and is fixedly connected to the driving gear (24); the driving gear (24) is meshed with the gear ring (26).

5. The human adipose tissue-derived matrix membrane preparation device according to claim 2, characterized in that: The base (1) has a built-in fourth motor, and the output shaft of the fourth motor is fixedly connected to the lower end of the rotating frame (11).

6. The human adipose tissue-derived matrix membrane preparation device according to claim 5, characterized in that: Also includes a film forming component; the film forming component includes: A first screw slide (31); the operating plate (9) has a first extension plate facing the other direction, which is inverted and fixedly arranged at the bottom of the first extension plate; An L-shaped arm (33), one end of which is mounted on the bottom of the first screw slide (31) and is driven to move by the first screw slide (31); The sampling device is mounted on the L-shaped arm (33), and includes a plurality of transfer tubes (16) arranged side by side, and an air extraction micro pump and an air charging micro pump respectively connected to the transfer tubes (16); the plurality of transfer tubes (16) are driven by a fourth motor and a lifting bracket to move into the working cylinder (21) for sampling; A plurality of pressing platforms (36) are evenly and linearly arranged along the moving direction of the first screw slide (31); each of the pressing platforms (36) is provided with a plurality of drainage holes; The second screw slide (30) and the third screw slide (32) are respectively mounted on both sides of the pressing platform (36); A movable platform (17) is mounted on the third screw slide (32); a support plate (34) is mounted on the top of the movable platform (17); A pressing groove (28), the back of which is connected to the support plate (34) via a pressing hydraulic cylinder (35); the groove body of the pressing groove (28) matches the outer contour of the pressing platform (36); Two placement grooves (29) are respectively arranged on the outside of the pressing platform (36) at both ends; filter paper (41) and mesh (42) are respectively placed in the two placement grooves (29); The paper picking assembly is mounted on the second screw slide (30) and is used to pick up and place the filter paper (41) and the mesh (42) on the pressing platform (36), and is driven to move by the second screw slide (30).

7. The human adipose tissue-derived matrix membrane preparation device according to claim 6, characterized in that: The paper picking assembly includes: A mounting block (39), the bottom of which is connected to the slide of the second screw slide (30) via a longitudinal telescopic rod (40); A limiting plate (15) is fixedly connected to the mounting block (39) at one end; two movable grooves are formed on the top of the limiting plate (15); A transverse telescopic rod (37) is fixedly mounted on the top of the mounting block (39); The tops of the two movable hooks (38) are fixedly connected to the transverse telescopic rod (37) and pass through the two movable slots respectively; two long slots cooperating with the movable hooks (38) are provided on both sides of the filter paper (41) and the mesh (42).

8. The human adipose tissue-derived matrix membrane preparation device according to claim 1, characterized in that: There are two groups of the working cylinder (21) and the metal mesh cylinder (20), one of which cooperates with the rubbing drive unit and the rubbing execution unit; Also included is a hydraulic fracturing assembly; the hydraulic fracturing assembly includes: Crushing hydraulic cylinder (13); the operating plate (9) further has a second extension plate in another direction, and the end of the crushing hydraulic cylinder (13) is fixedly arranged at the bottom of the second extension plate; The crushing hydraulic plate (14) is fixedly connected to the telescopic end of the crushing hydraulic cylinder (13), and cooperates with another set of the working cylinder (21) and the metal mesh cylinder (20).

9. The human adipose tissue-derived matrix membrane preparation device according to claim 1, characterized in that: The grinding rod (2) and the crushing rod (3) are both equipped with a drive motor; the drive motor drives the grinding rod (2) or the crushing rod (3) to rotate.

Images