Microorganism culture method for bioengineering

Abstract

The invention discloses a microbial culture method for bioengineering, and belongs to the technical field of bioengineering. A microorganism culture method for bioengineering comprises the following steps: S1, firstly, various culture media required for culturing microorganisms are introduced into an inner cavity of a culture vessel, the various culture media are flatly laid in the culture vessel, and then the microorganisms are introduced into the culture vessel; s2, sequentially placing a plurality of culture vessels filled with microorganisms on each multi-displacement mechanism in a culture box; s3, air in the box body of the cultivation box is heated, so that the temperature of the air in the cultivation box is gradually increased; by arranging the multi-displacement mechanism, microorganisms at different positions of each culture dish are uniformly heated while a plurality of culture dishes in the culture box are uniformly heated, so that the heating uniformity in the box body is improved, the heating time of the microorganisms at different positions is the same, and the accuracy of a microorganism culture test result is ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of bioengineering, and in particular to a method for culturing microorganisms for bioengineering. Background Art

[0002] Microbial culture refers to the rapid growth and reproduction of certain microorganisms with the help of artificially prepared culture media and artificially created culture conditions. It is called microbial culture. Microbial culture can be divided into pure culture and mixed culture. The former refers to the cultivation and utilization of a purified single strain of bacteria, and the latter refers to the cultivation of mixed strains or microorganisms in natural samples.

[0003] In existing microbial culture processes, microorganisms are typically placed in petri dishes, which are then placed in an incubator. The incubator must maintain appropriate temperature and humidity. When heating the incubator, the hot air inlet is typically located in one location, causing the petri dishes closest to the inlet to heat first. This reduces heating uniformity across multiple petri dishes within the incubator. Furthermore, varying heating times between petri dishes can affect microbial culture test results. Summary of the Invention

[0004] The purpose of the present invention is to solve the problems existing in the prior art and to propose a method for culturing microorganisms for bioengineering.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A method for culturing microorganisms for bioengineering, comprising the following steps:

[0007] S1: First, various culture media required for culturing microorganisms are introduced into the inner cavity of the culture dish, so that the various culture media are spread evenly in the culture dish, and then the microorganisms are introduced into the culture dish;

[0008] S2: Subsequently, multiple culture dishes containing microorganisms are sequentially placed on the multiple displacement mechanisms in the incubator;

[0009] S3: heating the air in the incubator to gradually increase the air temperature in the incubator;

[0010] S4: Controlling the operation of the multi-displacement mechanism so that the rotating component of the multi-displacement mechanism drives the culture dish to rotate in the incubator, so that the heating source in the incubator uniformly heats the multiple culture dishes in the incubator, and the swinging component of the multi-displacement mechanism drives the culture dish to move to multiple positions on the horizontal plane, thereby adjusting the position change of each position of the individual culture dish relative to the heating source in the incubator, so that the microorganisms at different positions inside the individual culture dish are uniformly heated;

[0011] S5: After the overall temperature of the air inside the incubator reaches the temperature required for microbial cultivation, further heating of the interior of the incubator is stopped.

[0012] Preferably, it includes an incubator, a driving motor is fixedly provided on the top of the incubator, a rotating rod connected to the output shaft of the driving motor is rotatably connected inside the incubator, a plurality of connecting plates are evenly arranged on the circumference of the rotating rod, a connecting ring is fixedly provided on one end of the connecting plate away from the rotating rod, each of the multi-displacement mechanisms is connected to the connecting ring in a one-to-one correspondence, the culture dish is arranged on the multi-displacement mechanism through a connecting piece, and the multi-displacement mechanism includes a rotating assembly for driving the culture dish to rotate and a swinging assembly arranged on the rotating assembly for driving the culture dish to move on the horizontal plane.

[0013] Preferably, the rotating assembly includes a movable gear rotatably connected to the bottom of the connecting ring and a gear ring meshing with the movable gear, and the gear ring is fixed to the inner wall of the incubator via a support rod.

[0014] Preferably, the swing assembly includes a first support plate fixed to the inner side wall of the movable gear, a transverse reciprocating screw rotatably connected to the first support plate, a first sleeve threadedly connected to the transverse reciprocating screw, and a first connecting plate fixed to the first sleeve, and the culture dish is connected to the first connecting plate through a connecting piece.

[0015] Preferably, the swing assembly also includes a second support plate fixed to the inner side wall of the movable gear, a longitudinal reciprocating screw rotatably connected to the second support plate, a second sleeve threadedly connected to the longitudinal reciprocating screw, and a second connecting plate fixed to the second sleeve, and the culture dish is connected to the second connecting plate via a connecting piece.

[0016] Preferably, the connecting member includes a moving block slidably connected to the first connecting plate and the second connecting plate, and a mounting plate fixed on the top of the moving block, and the culture dish is arranged on the mounting plate.

[0017] Preferably, a support plate is fixed on the lower side of the connecting plate, and the end of the support plate away from the connecting plate is connected to a fixed bevel gear coaxially arranged with the movable gear, the end of the transverse reciprocating screw is provided with a first bevel gear meshing with the fixed bevel gear, the transverse reciprocating screw is also provided with a second bevel gear, and the longitudinal reciprocating screw is provided with a third bevel gear meshing with the second bevel gear.

[0018] Preferably, a paddle for moving the air in the incubator is fixedly provided on the lower side of the connecting plate.

[0019] Preferably, a material hole is opened on one side of the incubator, a transparent cover is installed in the material hole, and a heat pipe for discharging hot air into the incubator is provided on the side of the incubator away from the material hole.

[0020] Preferably, a conical guide surface is provided on the top inner wall of the incubator, and a heat flow groove is further provided in the incubator, wherein the top opening and the bottom opening of the heat flow groove are both connected to the interior of the incubator body.

[0021] Compared with the prior art, the present invention provides a method for culturing microorganisms for bioengineering, which has the following beneficial effects:

[0022] 1. This bioengineering microbial cultivation method, by setting up a multi-displacement mechanism, can uniformly heat multiple culture dishes in the incubator while also uniformly heating the microorganisms at different positions in each culture dish, thereby improving the heating uniformity inside the box and making the heating time of microorganisms at various positions the same, thereby ensuring the accuracy of the microbial cultivation test results.

[0023] 2. This method for cultivating microorganisms for bioengineering uses controls the operation of a driving motor so that the output shaft of the driving motor drives a rotating rod to rotate. The rotating rod drives a movable gear to revolve orbitally through a connecting plate and a connecting ring. While the movable gear revolves orbitally, it engages with a gear ring on the inner wall of an incubator, causing the movable gear to revolve orbitally and rotate on its own simultaneously, thereby adjusting the positions of different culture dishes in the incubator while adjusting the positions of microorganisms in a single culture dish, thereby achieving uniform heating of the microorganisms in the incubator.

[0024] 3. The bioengineering microbial cultivation method drives the transverse reciprocating screw to rotate through the first support plate when the movable gear rotates. When the transverse reciprocating screw rotates, the first bevel gear and the fixed bevel gear are engaged and transmitted. The fixed bevel gear drives the transverse reciprocating screw and the second bevel gear to rotate. When the second bevel gear rotates, it is engaged and transmitted with the third bevel gear on the outside of the longitudinal reciprocating screw, thereby enabling the culture dish to move laterally and vertically on the horizontal plane while rotating itself. The position of the culture dish in the incubator is further adjusted so that each position of the culture dish can be moved to the air outlet of the heat pipe, thereby ensuring that microorganisms at different positions are evenly heated, thereby improving the accuracy of the microbial cultivation test results.

[0025] 4. This method for cultivating microorganisms for bioengineering uses a paddle plate provided on the lower side of a connecting plate. This allows the rotating rod to drive the culture dish to rotate while the paddle plate paddles the air in the incubator, thereby accelerating the diffusion of hot air entering the incubator, improving the uniformity of heating the air in the incubator, and accelerating the heating efficiency of the air inside the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0027] Figure 2 Schematic diagram of the internal structure of the incubator of the present invention;

[0028] Figure 3Schematic diagram of the cross-sectional structure of the incubator of the present invention;

[0029] Figure 4 It is a structural schematic diagram of the outer side of the rotating rod of the present invention;

[0030] Figure 5 The structure diagram of the multi-displacement mechanism of the present invention is shown in FIG. Figure 1 ;

[0031] Figure 6 The structure diagram of the multi-displacement mechanism of the present invention is shown in FIG. Figure 2 ;

[0032] Figure 7 For the present invention Figure 6 Schematic diagram of the partially enlarged structure of part A in the middle.

[0033] In the figure: 1. incubator; 101. material hole; 1011. transparent cover; 102. heat pipe; 103. conical guide surface; 104. heat flow groove; 2. drive motor; 201. rotating rod; 202. connecting plate; 203. connecting ring; 3. culture dish; 4. movable gear; 5. gear ring; 6. first support plate; 601. horizontal reciprocating screw; 6011. first bevel gear; 6012. second bevel gear; 602. first sleeve; 603. first connecting plate; 7. second support plate; 701. longitudinal reciprocating screw; 7011. third bevel gear; 702. second sleeve; 703. second connecting plate; 8. connector; 801. moving block; 802. mounting plate; 9. support plate; 901. fixed bevel gear; 10. dial plate. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0035] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the present invention.

[0036] Example 1: Reference Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 and Figure 7, a method for cultivating microorganisms for bioengineering, comprising the following steps:

[0037] S1: First, various culture media required for culturing microorganisms are introduced into the inner cavity of the culture dish 3, so that the various culture media are spread evenly in the culture dish 3, and then the microorganisms are introduced into the culture dish 3;

[0038] S2: Subsequently, multiple culture dishes 3 containing microorganisms are sequentially placed on the multiple displacement mechanisms in the incubator 1;

[0039] S3: heating the air in the incubator 1 so that the air temperature in the incubator 1 gradually increases;

[0040] S4: Controlling the operation of the multi-displacement mechanism so that the rotating component of the multi-displacement mechanism drives the culture dishes 3 to rotate in the incubator 1, thereby achieving uniform heating of the multiple culture dishes 3 in the incubator 1 by the heat source in the incubator 1, and causing the swinging component of the multi-displacement mechanism to drive the culture dishes 3 to move to multiple positions on the horizontal plane, thereby adjusting the position change of each position of the individual culture dishes 3 relative to the heat source in the incubator 1, thereby achieving uniform heating of microorganisms at different positions inside the individual culture dishes 3;

[0041] S5: After the overall temperature of the air inside the incubator 1 reaches the temperature required for microbial cultivation, further heating of the interior of the incubator 1 is stopped.

[0042] Reference Figure 1 、 Figure 2 、 Figure 3 and Figure 4 As a preferred technical solution of the present invention, further, a driving motor 2 is fixedly provided on the top of the incubator 1, and a rotating rod 201 connected to the output shaft of the driving motor 2 is rotatably connected inside the incubator 1. A plurality of connecting plates 202 are evenly arranged on the circumference of the rotating rod 201. A connecting ring 203 is fixedly provided on one end of the connecting plate 202 away from the rotating rod 201. Each multi-displacement mechanism is connected to the connecting ring 203 in a one-to-one correspondence. The culture dish 3 is arranged on the multi-displacement mechanism through a connecting piece 8. The multi-displacement mechanism includes a rotating assembly for driving the culture dish 3 to rotate and a rotating assembly for rotating the culture dish 3. A swinging assembly drives the culture dishes 3 to move on the horizontal plane; specifically, by controlling the operation of the driving motor 2, the output shaft of the driving motor 2 drives the rotating rod 201 to rotate in the incubator 1. When the rotating rod 201 rotates, it drives the connecting plate 202 and the connecting ring 203 to rotate, and the multi-displacement mechanism works automatically. While the rotating assembly uniformly heats the multiple culture dishes 3 in the incubator 1, the swinging assembly also uniformly heats the microorganisms at different positions of each culture dish 3, thereby improving the heating uniformity inside the box, making the heating time of microorganisms at various positions the same, and ensuring the accuracy of the microbial culture test results.

[0043] Reference Figure 1 、 Figure 2 、 Figure 3 and Figure 4 As a preferred technical solution of the present invention, further, the rotating assembly includes a movable gear 4 rotatably connected to the bottom of the connecting ring 203 and a gear ring 5 meshing with the movable gear 4, and the gear ring 5 is fixed to the inner wall of the incubator 1 through a support rod; specifically, when the rotating rod 201 rotates, the movable gear 4 is driven to revolve through the connecting plate 202 and the connecting ring 203, and the movable gear 4 is meshed with the gear ring 5 when revolving, so that the movable gear 4 drives the culture dish 3 to rotate, adjusts the position of the culture dish 3 in the box, and makes the microorganisms in each culture dish 3 move to the heating position of the box, thereby ensuring the uniformity of heating of the microorganisms inside the box.

[0044] Reference Figure 2 、 Figure 4 、 Figure 5 、 Figure 6 and Figure 7 As a preferred technical solution of the present invention, the swing assembly further includes a first support plate 6 fixed to the inner side wall of the movable gear 4, a transverse reciprocating screw 601 rotatably connected to the first support plate 6, a first sleeve 602 threadedly connected to the transverse reciprocating screw 601, and a first connecting plate 603 fixed to the first sleeve 602, and the culture dish 3 is connected to the first connecting plate 603 through a connecting member 8.

[0045] Furthermore, the swing assembly also includes a second support plate 7 fixed to the inner wall of the movable gear 4, a longitudinal reciprocating screw 701 rotatably connected to the second support plate 7, a second sleeve 702 threadedly connected to the longitudinal reciprocating screw 701, and a second connecting plate 703 fixed on the second sleeve 702. The culture dish 3 is connected to the second connecting plate 703 through a connecting member 8.

[0046] Furthermore, the connecting member 8 includes a moving block 801 slidably connected to the first connecting plate 603 and the second connecting plate 703 , and a mounting plate 802 fixed on the top of the moving block 801 , and the culture dish 3 is set on the mounting plate 802 .

[0047] Furthermore, a support plate 9 is fixed to the lower side of the connecting plate 202, and the end of the support plate 9 away from the connecting plate 202 is connected to a fixed bevel gear 901 coaxially arranged with the movable gear 4, and the end of the transverse reciprocating screw 601 is provided with a first bevel gear 6011 that meshes with the fixed bevel gear 901, and the transverse reciprocating screw 601 is also provided with a second bevel gear 6012, and the longitudinal reciprocating screw 701 is provided with a third bevel gear 7011 that meshes with the second bevel gear 6012.

[0048] Specifically, when the movable gear 4 rotates, it drives the transverse reciprocating screw 601 to rotate through the first support plate 6. When the transverse reciprocating screw 601 rotates, it drives the first bevel gear 6011 to engage and transmit with the fixed bevel gear 901. The fixed bevel gear 901 drives the transverse reciprocating screw 601 and the second bevel gear 6012 to rotate. When the second bevel gear 6012 rotates, it engages and transmits with the third bevel gear 7011 on the outside of the longitudinal reciprocating screw 701, thereby enabling the culture dish 3 to move laterally and vertically on the horizontal plane while rotating itself, and further adjusting the position of the culture dish 3 in the incubator 1, so that each position of the culture dish 3 can be moved to the heat source inside the incubator 1, ensuring that the microorganisms at different positions are evenly heated, thereby improving the accuracy of the microbial culture test results.

[0049] Reference Figure 2 and Figure 4 As a preferred technical solution of the present invention, a paddle plate 10 for stirring the air in the incubator 1 is further fixedly provided on the lower side of the connecting plate 202; specifically, by arranging the paddle plate 10 on the lower side of the connecting plate 202, the rotating rod 201 drives the culture dish 3 to rotate while the paddle plate 10 stirs the air in the incubator 1, thereby accelerating the diffusion of the hot air entering the incubator 1, improving the uniformity of the heating of the air in the incubator 1, and accelerating the heating efficiency of the air inside the incubator 1.

[0050] Reference Figure 1 、 Figure 2 and Figure 3 As a preferred technical solution of the present invention, further, a material hole 101 is opened on one side of the incubator 1, and a transparent cover 1011 is installed in the material hole 101. A heat pipe 102 for discharging hot air into the incubator 1 is provided on the side of the incubator 1 away from the material hole 101; specifically, by providing the material hole 101, it is convenient to place the culture dish 3 in the incubator 1, and the provision of the transparent cover 1011 makes it convenient for the staff to observe the cultivation status of the microorganisms in the culture dish 3. The heat pipe 102 can introduce hot air into the incubator 1, so that the temperature in the incubator 1 reaches the temperature required for microbial cultivation.

[0051] Reference Figure 2 and Figure 4As a preferred technical solution of the present invention, further, a conical guide surface 103 is provided on the top inner wall of the incubator 1, and a heat flow groove 104 is also provided in the incubator 1, and the top opening and the bottom opening of the heat flow groove 104 are both connected to the interior of the box body of the incubator 1; specifically, the hot air in the incubator 1 rises to the top inner wall of the incubator 1, and the top inner wall of the incubator 1 is provided with a conical guide surface 103, which is convenient for guiding the hot air rising to the top of the incubator 1, and the rising hot air enters from the upper end opening of the heat flow groove 104, and as the hot air continues to rise, the hot air flows in the heat flow groove 104 and re-enters the interior of the box body of the incubator 1 from the bottom of the heat flow groove 104, so that the hot air continuously flows up and down in the incubator 1, thereby accelerating the heating efficiency of the air in the incubator 1.

[0052] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A method for cultivating microorganisms for bioengineering, characterized in that: The following steps are involved: S1: First, various culture media required for culturing microorganisms are introduced into the inner cavity of the culture dish (3), so that the various culture media are spread evenly in the culture dish (3), and then the microorganisms are introduced into the culture dish (3); S2: Subsequently, a plurality of culture dishes (3) containing microorganisms are sequentially placed on the respective multi-displacement mechanisms in the incubator (1); S3: heating the air in the incubator (1) so that the temperature of the air in the incubator (1) gradually rises; S4: Control the operation of the multi-displacement mechanism so that the rotating component of the multi-displacement mechanism drives the culture dish (3) to rotate in the incubator (1), so that the heat source in the incubator (1) uniformly heats the multiple culture dishes (3) in the incubator (1), and the swing component of the multi-displacement mechanism drives the culture dish (3) to move to multiple positions on the horizontal plane, thereby adjusting the position change of each position of the single culture dish (3) relative to the heat source in the incubator (1), so that the microorganisms at different positions inside the single culture dish (3) are uniformly heated; S5: After the overall temperature of the air inside the incubator (1) reaches the temperature required for microbial cultivation, further heating of the inside of the incubator (1) is stopped.

2. A method for culturing microorganisms for bioengineering according to claim 1, characterized in that: The invention comprises an incubator (1), characterized in that a driving motor (2) is fixedly provided on the top of the incubator (1), a rotating rod (201) connected to the output shaft of the driving motor (2) is rotatably connected inside the incubator (1), a plurality of connecting plates (202) are evenly arranged on the circumference of the rotating rod (201), a connecting ring (203) is fixedly provided on one end of the connecting plate (202) away from the rotating rod (201), each of the multi-displacement mechanisms is connected to the connecting ring (203) in a one-to-one correspondence, the culture dish (3) is arranged on the multi-displacement mechanism through a connecting member (8), and the multi-displacement mechanism comprises a rotating assembly for driving the culture dish (3) to rotate and a swinging assembly arranged on the rotating assembly for driving the culture dish (3) to move on a horizontal plane.

3. A method for culturing microorganisms for bioengineering according to claim 2, characterized in that: The rotating assembly comprises a movable gear (4) rotatably connected to the bottom of the connecting ring (203) and a gear ring (5) meshingly connected to the movable gear (4); the gear ring (5) is fixed to the inner wall of the incubator (1) via a support rod.

4. A method for culturing microorganisms for bioengineering according to claim 3, characterized in that: The swing assembly comprises a first support plate (6) fixed to the inner side wall of the movable gear (4), a transverse reciprocating screw (601) rotatably connected to the first support plate (6), a first sleeve (602) threadedly connected to the transverse reciprocating screw (601), and a first connecting plate (603) fixed to the first sleeve (602), and the culture dish (3) is connected to the first connecting plate (603) via a connecting member (8).

5. A method for culturing microorganisms for bioengineering according to claim 4, characterized in that: The swing assembly further comprises a second support plate (7) fixed to the inner side wall of the movable gear (4), a longitudinal reciprocating screw (701) rotatably connected to the second support plate (7), a second sleeve (702) threadedly connected to the longitudinal reciprocating screw (701), and a second connecting plate (703) fixed to the second sleeve (702), and the culture dish (3) is connected to the second connecting plate (703) via a connecting member (8).

6. A method for culturing microorganisms for bioengineering according to claim 5, characterized in that: The connecting member (8) comprises a moving block (801) slidably connected to the first connecting plate (603) and the second connecting plate (703), and a mounting plate (802) fixed on the top of the moving block (801), and the culture dish (3) is arranged on the mounting plate (802).

7. A method for culturing microorganisms for bioengineering according to claim 6, characterized in that: A support plate (9) is fixedly provided on the lower side of the connecting plate (202); an end of the support plate (9) away from the connecting plate (202) is connected to a fixed bevel gear (901) coaxially arranged with the movable gear (4); a first bevel gear (6011) meshing with the fixed bevel gear (901) is provided at the end of the transverse reciprocating screw (601); a second bevel gear (6012) is further provided on the transverse reciprocating screw (601); and a third bevel gear (7011) meshing with the second bevel gear (6012) is provided on the longitudinal reciprocating screw (701).

8. A method for culturing microorganisms for bioengineering according to claim 7, characterized in that: A shifting plate (10) for shifting the air in the incubator (1) is fixedly provided on the lower side of the connecting plate (202).

9. A method for culturing microorganisms for bioengineering according to claim 8, characterized in that: A material hole (101) is provided on one side of the incubator (1), a transparent cover plate (1011) is installed in the material hole (101), and a heat pipe (102) for discharging hot air into the incubator (1) is provided on a side of the incubator (1) away from the material hole (101).

10. The method for culturing microorganisms for bioengineering according to claim 9, characterized in that: A conical flow guide surface (103) is provided on the top inner wall of the incubator (1), and a heat flow groove (104) is also provided inside the incubator (1), wherein the top opening and the bottom opening of the heat flow groove (104) are both connected to the interior of the incubator (1).

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