Herbaceous plant composition beverage capable of dredging collaterals and relieving blockage and preparation method of herbaceous plant composition beverage

Abstract

The invention provides a herbaceous plant composition beverage capable of dredging collaterals and relieving blockage and a preparation method thereof. Belongs to health-care food. The tea comprises the following components: rhizoma polygonati, cistanche tubulosa, saffron crocus, rhizoma phragmitis, radix puerariae, semen sterculiae lychnophorae On the basis of the traditional Chinese medicine Qi-blood-body fluid theory and the meridian theory, the aim of dredging vessels and relieving blockage is achieved through multiple mechanisms of benefiting qi and nourishing blood, promoting blood circulation to remove blood stasis, conditioning qi activity and body fluid and the like. The compatibility of the traditional Chinese medicines embodies the characteristics of combining tonifying and dredging, treating internal organs simultaneously and being mild in medicine property, and the traditional Chinese medicine composition has an excellent conditioning value on fatigue and oxygen resistance reduction caused by deficiency of qi and blood and stasis. Meanwhile, a modern extraction method is matched to enrich the effective components; the herbaceous plant composition beverage with excellent effects is prepared.

Description

Technical Field

[0001] The invention belongs to the field of health-care foods, and in particular relates to a herbal plant composition drink capable of unblocking blood vessels and removing blockages, and a preparation method thereof. Background Art

[0002] Against the backdrop of an increasingly fast pace of modern life and increasing pressure, people are becoming more and more prone to poor blood circulation and blood vessel blockages due to long-term lack of exercise, irregular diet, disordered work and rest, and other bad living habits.

[0003] In Traditional Chinese Medicine (TCM), meridians are channels through which qi and blood flow, like crisscrossing rivers that nourish the organs and tissues throughout the body. Qi, the leader of blood, propels blood through the vessels; blood, the mother of qi, carries qi to nourish the body. When qi and blood are in harmony and the meridians are unobstructed, the body is full of energy, organs function normally, and the body exhibits excellent health. However, factors such as external pathogenic qi, emotional imbalance, improper diet, and inappropriate work and rest can hinder the smooth flow of qi and blood, leading to blood stasis.

[0004] This "blockage" can lead to a series of health problems. From the perspective of fatigue, stagnant Qi and blood prevent the internal organs and tissues from receiving adequate nutrition, hindering energy metabolism and making the body prone to fatigue, manifesting as mental fatigue and weakness in the limbs. In terms of sleep, poor Qi and blood circulation can affect peace of mind, leading to insomnia, dreaminess, shallow sleep, and easy awakening. Traditional Chinese Medicine believes that "when Yang enters Yin, sleep occurs." Qi and blood stasis interfere with the normal exchange of Yin and Yang Qi, thereby disrupting sleep rhythms. In terms of tolerance to hypoxia, Qi and blood cannot smoothly transport oxygen to all parts of the body. Cell function declines due to hypoxia, and the body's tolerance to hypoxic environments also decreases. Therefore, "unblocking the meridians and unblocking blockages" is an important foundation for maintaining human health and improving fatigue, sleep, and other conditions.

[0005] Modern medicine closely links the artery's ability to clear blockages with the circulatory system. Blood vessels are key pathways for transporting oxygen and nutrients and removing metabolic waste. When vascular problems such as atherosclerosis, stenosis, and thrombosis develop, blood circulation is impaired, impacting the normal function of tissues and organs.

[0006] Poor blood circulation prevents muscle tissue from receiving sufficient oxygen and nutrients. Meanwhile, metabolic waste products like lactic acid cannot be removed promptly, accumulating in the body, leading to increased muscle soreness and fatigue. This in turn reduces fatigue resistance. Insufficient blood flow to the brain can affect the normal secretion and regulation of neurotransmitters, disrupting the function of the sleep center and causing sleep disorders. Blood vessel blockage or increased blood viscosity reduces the blood's ability to carry and deliver oxygen, making organs more vulnerable to damage in hypoxic environments and reducing their ability to withstand hypoxia.

[0007] Herbal plants contain rich medicinal ingredients, and many types have the effect of clearing meridians and relieving blockages, such as salvia miltiorrhiza, saffron, Chuanxiong and other blood-activating and blood-stasis-removing herbs; astragalus, angelica, Codonopsis and other qi-invigorating and blood-nourishing herbs; how to extract or enrich the effective ingredients among them through modern scientific methods is an important topic in the current modernization of Chinese medicinal materials. Summary of the Invention

[0008] The purpose of the present invention is to provide a herbal plant composition drink capable of unblocking meridians and removing blockages and a preparation method thereof, so as to develop and utilize traditional Chinese medicinal materials with modern means.

[0009] A herbal plant composition drink capable of clearing meridians and relieving blockages, comprising the following components in parts by mass:

[0010]

[0011] The preparation method of the reed rhizome, kudzu root and pterostilbene is as follows: the corresponding plant raw materials are fermented with Lactobacillus reuteri, extracted with water, and then freeze-dried;

[0012] The preparation method of saffron and polygonatum is as follows: firstly extracting with water, freeze-drying the water extract; and extracting the solid matter after water extraction with supercritical carbon dioxide;

[0013] The Cistanche tubulosa is an extract that has been subjected to supercritical carbon dioxide treatment;

[0014] The above extracts are mixed with black tea to obtain a herbal plant composition drink capable of clearing meridians and relieving blockages.

[0015] Preferably, the preparation method of the reed rhizome, kudzu root and sterculia lychnophora is as follows: taking fresh reed rhizome, fresh kudzu root and fresh sterculia lychnophora; crushing to at least 80 mesh; then steaming and sterilizing to obtain the substrate;

[0016] The fermentation parameters of the activated Lactobacillus reuteri after inoculation of the substrate are as follows: temperature 37-40°C; initial pH 6.4±0.2; anaerobic fermentation; fermentation for at least 48 hours; then the fermentation is considered complete if the pH change is ≤0.15 / h;

[0017] After the fermentation is completed, the product is sterilized and centrifuged to obtain a supernatant and a solid portion. The solid portion is subjected to cell disruption treatment and then water-extracted at a pH of 6-7, at least 50° C., and a solid-liquid ratio of 1: at least 10; the water extract is taken; the water extract and the supernatant are combined and then freeze-dried.

[0018] Preferably, the preparation method of saffron is:

[0019] The upper part and stigma of saffron are taken and crushed to at least 40 mesh; the mixture is extracted with water at a temperature of 70-80°C and a solid-liquid ratio of 1:15 or more for at least 1 hour; the mixture is filtered while hot to separate the solid and liquid, and the filtrate is freeze-dried; the solid is set aside;

[0020] The saffron solids are dried to a moisture content of ≤8%; then supercritical carbon dioxide extraction is performed; and after the extraction is complete, the entrainer is removed;

[0021] The conditions for supercritical extraction are:

[0022] ≥35MPa, 45-55°C, carbon dioxide flow rate ≥6L / min; and at least 10% ethanol is added as an entrainer.

[0023] Preferably, the preparation method of the polygonatum is:

[0024] Select fresh tubers of Polygonatum sibiricum, steam and dry at low temperature, then crush to at least 40 mesh; extract with water at a temperature of 90-95°C and a solid-liquid ratio of 1:15 or more for at least 1-2 hours; filter while hot to separate the solid and liquid, and freeze-dry the filtrate; and set aside the solid;

[0025] The solid matter of Polygonatum sibiricum is dried to a water content of ≤8%; then subjected to supercritical carbon dioxide extraction; after the extraction is complete, the entrainer is removed;

[0026] The conditions for supercritical extraction are:

[0027] ≥35MPa, 45-55°C, carbon dioxide flow rate ≥6L / min; and at least 10% ethanol is added as an entrainer.

[0028] Preferably, the preparation method of the Cistanche tubulosa is:

[0029] Select fresh fleshy stems of Cistanche tubulosa; crush them and pass them through at least 8 mesh; then dry them to a moisture content of ≤8%; then perform supercritical carbon dioxide extraction; and remove the entrainer after the extraction is complete.

[0030] The conditions for supercritical extraction are:

[0031] ≥35MPa, 45-55°C, carbon dioxide flow rate ≥6L / min; and at least 10% ethanol is added as an entrainer.

[0032] Preferably, the Cistanche tubulosa is enzymatically hydrolyzed with cellulase before drying.

[0033] The present invention also provides the use of a herbal plant composition drink capable of clearing blood vessels and removing blockages in the preparation of a health product; the health product is a substitute tea.

[0034] Furthermore, in order to improve the taste and enhance the related efficacy, the present invention can also add at least one of the following ingredients: five-fingered peach, ophiopogon japonicus, red lotus seeds, gorgon fruit, lily, wax gourd peel, corn silk, ganoderma lucidum, dandelion, small green tangerine peel, tangerine peel, barley tea, and dendrobium flower.

[0035] In the present invention:

[0036] The polysaccharides in Polygonatum can promote energy metabolism, enhance the body's resistance to fatigue, regulate the immune system, and reduce fatigue.

[0037] The phenylethanoid glycoside compounds contained in Cistanche tubulosa can improve physical endurance and athletic ability. At the same time, it can regulate the function of the hypothalamic-pituitary-adrenal axis and relieve hormonal disorders in fatigue states.

[0038] Saffron contains flavonoids and carotenoids (primarily crocin); crocin has therapeutic effects on a variety of central nervous system and cardiovascular system diseases, and also has anti-cancer, anti-inflammatory, antioxidant, hepatoprotective, and anti-diabetic properties. Flavonoids, such as safranin and kaempferol, have antioxidant, anti-inflammatory, and anti-allergic effects, helping the body defend against free radicals and reduce inflammatory responses. They can also improve the body's tolerance to hypoxia.

[0039] Modern research shows that the polysaccharides and amino acids contained in reed rhizome can regulate the body's metabolic function and to a certain extent help improve cell function under hypoxic conditions.

[0040] The puerarin in Pueraria root can improve blood circulation, increase oxygen and nutrient supply to muscle tissue, and accelerate lactic acid metabolism, thereby reducing muscle fatigue after exercise. In addition, the flavonoids it contains have antioxidant effects and can reduce the damage of free radicals to cells.

[0041] The amino acids and trace elements contained in Plantago ovata regulate the body's overall metabolism and support the improvement of oxygen tolerance.

[0042] Black tea contains caffeine and tea polyphenols. Caffeine can stimulate the central nervous system, enhance alertness and energy, and temporarily relieve fatigue; tea polyphenols have antioxidant effects, helping to eliminate free radicals in the body and reduce fatigue-related oxidative stress damage.

[0043] From a Traditional Chinese Medicine (TCM) perspective, "the meridians function by being unobstructed." If Qi and blood are deficient, their flow is weak, easily leading to blood stasis. If pathological factors such as Qi stagnation, cold stagnation, and phlegm-dampness block the meridians, this can also cause a blockage. As stated in the "Lingshu: Meridians," "The meridians determine life and death, treat all ailments, and regulate deficiency and excess. They must be unobstructed." Fatigue and decreased oxygen tolerance are often associated with Qi and blood deficiency and blocked meridians. Because "Qi is the commander of blood, and blood is the mother of Qi," Qi and blood deficiency leads to malnutrition of the body, and hypoxia makes symptoms like fatigue and shortness of breath more likely to occur.

[0044] In the present invention, Polygonatum sibiricum and Cistanche tubulosa are used to tonify deficiency and treat the root cause, while saffron and Pueraria root are used to promote blood circulation and treat the symptoms. The combination of the two can not only tonify the deficiency of qi and blood, but also remove the stagnation of blood vessels, which is in line with the treatment principle of traditional Chinese medicine of "tonifying the deficiency and clearing the stasis", avoiding the loss of vital energy by simply clearing the stasis, or the retention of evil and causing blockage by simply nourishing.

[0045] From the perspective of internal organs, tonifying the kidney (Cistanche deserticola) warms the yang and unclogs the meridians, tonifying the spleen (Polygonatum sibiricum, black tea) replenishes qi and produces blood, soothing the liver (saffron invigorates blood circulation and regulates qi) relieves and promotes smooth flow, and clearing the lungs (reed root, Sterculia lychnophora) regulates the water channels. Through the coordinated conditioning of multiple internal organs, the normal distribution of qi, blood, and body fluids is restored, fundamentally improving the congestion in the meridians.

[0046] The formula combines warm herbs (Cistanche deserticola, saffron, black tea) with cool herbs (Phragmites australis, Sterculia lychnophora, Pueraria lobata) to counteract the cold and heat, avoiding damage to yin by warm and dryness or damage to yang by cold and coolness; Qi-invigorating herbs (Potamogeton sibiricum) are combined with blood-activating herbs (saffron), so that when Qi flows, blood flows, and when blood activates, Qi thrives, maintaining a balance between Yin and Yang, making it suitable for conditioning people of most physical conditions.

[0047] The present invention uses different extraction methods according to the solubility differences of the active ingredients in different herbal medicinal materials. Among them, the main active ingredients of Phragmites australis, Pueraria root and Sterculia lychnophora are water-soluble substances such as polysaccharides and flavonoid glycosides, so they are extracted using water extraction.

[0048] In addition, the present invention uses Lactobacillus reuteri fermentation to decompose plant cell walls, increase the dissolution rate of flavonoids (such as puerarin) and polysaccharides, and generate prebiotic metabolites (such as short-chain fatty acids) to enhance anti-inflammatory activity.

[0049] Saffron and Polygonatum sibiricum contain water-soluble crocin, some flavonoids, and fat-soluble crocetin, β-carotene, zeaxanthin, lycopene and other ingredients; therefore, water extraction is performed first and then supercritical carbon dioxide extraction; however, the present invention has found that if Lactobacillus reuteri fermentation is also used for pretreatment, the extraction rate of fat-soluble active ingredients will be greatly reduced; therefore, fermentation is not used.

[0050] The main active ingredients of Cistanche tubulosa are phenylethanoid glycosides (echinacoside) and sterols; therefore, the carbon dioxide supercritical extraction method is used.

[0051] The beneficial technical effects of the present invention are at least as follows:

[0052] This invention, based on the Traditional Chinese Medicine theory of Qi, Blood, and Body Fluids and the Meridian Theory, achieves the goal of "unblocking meridians and unblocking blockages" through multiple mechanisms, including replenishing Qi and nourishing blood, promoting blood circulation and removing blood stasis, and regulating Qi and body fluids. Its combination embodies the characteristics of "combining tonification and unblocking, treating both internal organs, and possessing a balanced medicinal property," offering excellent therapeutic value for fatigue and decreased oxygen tolerance caused by Qi and blood deficiency and stasis. Furthermore, modern extraction methods are employed to enrich the active ingredients, resulting in a highly effective herbal beverage combination. DETAILED DESCRIPTION

[0053] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making any creative efforts shall fall within the scope of protection of the present invention.

[0054] In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of the technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include one or more of the specified features. In the description of the present invention, "plurality" means two or more, unless otherwise specifically defined.

[0055] Unless otherwise specified, the experimental methods used in the specific embodiments are all conventional methods, and the materials, reagents, etc. used are all commercially available unless otherwise specified.

[0056] Unless otherwise specified, %, and percentages are all mass percentages.

[0057] Some of the raw materials used in the present invention come from the following sources:

[0058] Lactobacillus reuteri: commercially available Lactobacillus reuteri CCFM8631, with a strain concentration ≥1×107 CFU / g.

[0059] Polygonatum: The rhizome of Polygonatum sibiricum Red., a plant of the Liliaceae family.

[0060] Cistanche tubulosa: The fleshy stem with scale leaves of Cistanche tubulosa (Schenk) Wight.

[0061] Saffron: The dried stigma of Crocus sativus L., a plant of the Iridaceae family.

[0062] Reed rhizome; the fresh rhizome of the grass plant Phragmites communis Trin.

[0063] Pueraria root: the root of Pueraria lobata (Willd.) Ohwi, a plant of the Leguminosae family.

[0064] Sterculia lychnophora: the mature seeds of Sterculia lychnophora Hance, a plant of the Sterculiaceae family.

[0065] Black tea: Commercially available fully fermented tea.

[0066] Five-fingered ginseng, Ophiopogon japonicus, red lotus seeds, Euryale ferox, lily, wax gourd peel, corn silk, Ganoderma lucidum, dandelion, small green tangerine peel, tangerine peel, barley tea, dendrobium flower, etc. are all standard Chinese medicinal materials recorded in the "Pharmacopoeia 2020 Edition".

[0067] Example 1

[0068] The fermentation experiment of Phragmites australis, Pueraria root and Sterculia lychnophora includes the following steps:

[0069] S1: Take fresh reed rhizome, fresh kudzu root and fresh sterculia lychnophora respectively; then steam at 100°C for 20 minutes to sterilize, and crush to at least 80 mesh; obtain the respective substrates;

[0070] S2: The substrate is inoculated with activated Lactobacillus reuteri for fermentation, with an inoculation amount of 5%.

[0071] Fermentation parameters were: temperature 37-40°C; initial pH 6.4±0.2; anaerobic fermentation; fermentation for at least 48 hours; and fermentation was considered complete when the pH change was ≤0.15 / h.

[0072] The substrate further includes 20% yeast extract FM 528, 4% anhydrous sodium acetate, 1.2% magnesium sulfate heptahydrate, 0.5% hydrated magnesium sulfate, and 0.5% Tween 80.

[0073] S3: After fermentation, the mixture was sterilized by heating at 90°C for 30 minutes; centrifuged at 8000 rpm and 4°C for 15 minutes; the supernatant and solid fractions were separated, the solid fraction was subjected to ultrasonic cell disruption, and then extracted with water at a pH of 6-7, at least 50°C, and a solid-to-liquid ratio of 1:10; the water extract was filtered through a 0.45 μm microfiltration membrane;

[0074] The S4 aqueous extract and the supernatant were combined, then freeze-dried and stored in a sealed container.

[0075] Example 2

[0076] Extraction experiment of Phragmites australis, Pueraria root and Sterculia lychnophora; including the following steps:

[0077] S1: Take fresh reed rhizome, fresh kudzu root, and fresh sterculia lychnophora, steam them at 100°C for 20 minutes to sterilize, crush them to a minimum of 80 mesh, and then add 4 times the amount of deionized water to each to make a solution;

[0078] S3: The solution was centrifuged at 8000 rpm and 4°C for 15 minutes to separate the supernatant and solid fractions. The solid fraction was subjected to ultrasonic cell disruption and then subjected to water extraction at a pH of 6-7, at least 50°C, and a solid-to-liquid ratio of 1:10. The water extract was filtered through a 0.45 μm microfiltration membrane.

[0079] The S4 aqueous extract and the supernatant were combined, then freeze-dried and stored in a sealed container.

[0080] Example 3

[0081] The water extraction experiment of saffron and polygonatum includes the following steps:

[0082] The upper part of the style and stigma of saffron are taken and crushed to at least 40 mesh; the saffron is extracted with water at a temperature of 70-80°C and a solid-liquid ratio of 1:15 for 1 hour; the solid-liquid separation is carried out by hot filtration, and the water extraction filtrate is freeze-dried to obtain the saffron water extract; the saffron solid is sealed separately for later use;

[0083] Fresh tubers of Polygonatum sibiricum are selected, steamed at 100°C for 10 minutes, and then dried at 60°C to a moisture content of ≤15%; then crushed to at least 40 mesh; water extracted for 12 hours at a temperature of 90-95°C and a solid-liquid ratio of 1:15; filtered while hot for solid-liquid separation, and freeze-dried the water extraction filtrate to obtain a water extract of Polygonatum sibiricum; the Polygonatum sibiricum solid is sealed separately for later use.

[0084] Example 4

[0085] The supercritical extraction experiment of saffron, polygonatum and cistanche tubulosa includes the following steps:

[0086] The saffron solids are dried to a moisture content of ≤8%; then supercritical carbon dioxide extraction is performed; after the extraction is complete, the pressure is reduced and the ethanol is separated, and then the extract is purged with nitrogen to remove the entrainer;

[0087] The conditions for supercritical extraction are:

[0088] ≥35MPa, 45-55°C, carbon dioxide flow rate ≥6L / min; and 10% pure ethanol is added as an entrainer.

[0089] The solid matter of Polygonatum sibiricum is dried to a water content of ≤8%; then supercritical carbon dioxide extraction is performed; after the extraction is completed, the pressure is reduced and the ethanol is separated, and then the extract is purged with nitrogen to remove the entrainer;

[0090] The conditions for supercritical extraction are:

[0091] ≥35MPa, 45-55°C, carbon dioxide flow rate ≥6L / min; and at least 10% ethanol is added as an entrainer.

[0092] Select fresh fleshy stems of Cistanche tubulosa; crush and pass through at least 8 mesh; then dry to a moisture content of ≤8%; then perform supercritical carbon dioxide extraction; after extraction, depressurize and separate ethanol, and then purge the extract with nitrogen to remove entrainers.

[0093] The conditions for supercritical extraction are:

[0094] ≥35MPa, 45-55°C, carbon dioxide flow rate ≥6L / min; and at least 10% ethanol is added as an entrainer.

[0095] Example 5 Comparative experiment on the content of active ingredients

[0096] 1. According to the Chinese Pharmacopoeia 2020 Edition, the active ingredients of Phragmites australis, Pueraria lobata, and Sterculia lychnophora were tested;

[0097] (1) Ultra-performance liquid chromatography (UPLC) was used to measure the contents of chlorogenic acid and luteolin in Phragmites australis.

[0098] Chromatographic conditions: ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm), mobile phase: acetonitrile-0.1% formic acid water (gradient elution), flow rate: 0.3 mL / min, detection wavelength: 280 nm (chlorogenic acid), 350 nm (luteolin).

[0099] (2) Puerarin (C) was measured according to the method described under the entry of “Puerariae”. 21 H 20 O9) content.

[0100] (3) The polysaccharide content of Sterculia lychnophora was measured by the phenol-sulfuric acid method, in which the monosaccharide generated by polysaccharide hydrolysis reacted with a color developer and the absorbance was measured at 490 nm. The flavonoid content was measured by high performance liquid chromatography (HPLC).

[0101] High performance liquid chromatography (HPLC) conditions: C18 column (250 mm×4.6 mm, 5 μm), mobile phase: methanol-0.1% aqueous phosphoric acid solution (gradient elution), flow rate: 1.0 mL / min, detection wavelength: 254 nm.

[0102] Since the aforementioned reed rhizome, kudzu root and sterculia lychnophora are in the form of freeze-dried powder, they need to be re-dissolved into aqueous solution and then the supernatant is extracted for sampling; reed rhizome is re-dissolved with 50% methanol aqueous solution; sterculia lychnophora is re-dissolved with 80% ethanol; kudzu root is re-dissolved according to the method of "Chinese Pharmacopoeia 2020 Edition".

[0103] The results are shown below.

[0104] Example 1 Phragmites australis freeze-dried powder - chlorogenic acid content: 5.87%

[0105] Example 2 Phragmites australis freeze-dried powder - chlorogenic acid content: 4.34%

[0106] Example 1 Phragmites australis freeze-dried powder - luteolin content: 2.82%

[0107] Example 2 Phragmites australis freeze-dried powder - luteolin content: 2.64%

[0108] Example 1 Pueraria lobata freeze-dried powder - puerarin content: 22.4%

[0109] Example 2 Pueraria lobata freeze-dried powder - puerarin content: 19.7%

[0110] Example 1: Freeze-dried Sterculia lycopersicum powder - polysaccharide content: 78.6%

[0111] Example 2: Freeze-dried Sterculia lycopersicum powder - polysaccharide content: 64.3%

[0112] Example 1: Freeze-dried Sterculia lycopersicum powder - flavonoid content: 8.8%

[0113] Example 2: Freeze-dried Sterculia lycopersicum powder - flavonoid content: 5.6%

[0114] 2. Detection of water-soluble components of saffron and polygonatum

[0115] (1) Crocin-I, crocin-II and crocin-picrocephalin were detected in saffron according to the entry of saffron in the Chinese Pharmacopoeia 2020.

[0116] (2) The content of Polygonatum sibiricum polysaccharide was detected in Polygonatum sibiricum, and the detection method was in accordance with the "Chinese Pharmacopoeia 2020 Edition".

[0117] The test samples are from the saffron water extract and the polygonatum water extract of Example 3.

[0118] The results are shown below.

[0119] Example 3 Saffron water extract - crocin-I, crocin-II total content: 46.7%

[0120] Example 3 Saffron water extract - crocin content: 22.4%

[0121] Example 3 Polygonatum sibiricum water extract - Polygonatum sibiricum polysaccharide content: 24.5%

[0122] 3. Detection of fat-soluble components in saffron, polygonatum, and cistanche tubulosa

[0123] (1) Detection of crocetin by high performance liquid chromatography (HPLC)

[0124] Chromatographic column: C18 reverse phase column (such as 250mm×4.6mm, 5μm), suitable for separating fat-soluble components.

[0125] Mobile phase: methanol-0.1% phosphoric acid aqueous solution (85:15, v / v).

[0126] Detection wavelength: 440 nm was used as the detection wavelength.

[0127] Column temperature: 30°C, flow rate: 1.0 mL / min

[0128] Injection volume: 10 μL.

[0129] (2) Polygonatum sibiricum was measured using the HPLC-UV method; the chromatographic conditions were:

[0130] Chromatographic column: Waters XBridge C18 (250 mm × 4.6 mm, 5 μm);

[0131] Mobile phase: methanol-water (88:12, v / v), isocratic elution;

[0132] Flow rate: 1.0 mL / min, column temperature: 35°C, detection wavelength: 203 nm;

[0133] Injection volume: 10 μL.

[0134] (3) Cistanche tubulosa: According to the Chinese Pharmacopoeia 2020 edition, echinacoside (C 35 H 46 O 20 ) and verbascoside (C 29 H 36 O 15 )’s total amount.

[0135] Since the aforementioned saffron, polygonatum, and cistanche tubulosa are freeze-dried powders, they need to be reconstituted into aqueous solutions, and the supernatant is extracted for sampling. Polygonatum is reconstituted in 50% methanol-water solution. Saffron and cistanche tubulosa are reconstituted according to the method in the "Chinese Pharmacopoeia 2020 Edition."

[0136] The test samples are from the supercritical extract of saffron, the supercritical extract of polygonatum and the supercritical extract of cistanche tubulosa in Example 4.

[0137] The results are shown below.

[0138] Example 4 Saffron supercritical extract - crocetin content: 6.8%.

[0139] Example 4 Polygonatum sibiricum supercritical extract - sapogenin content: 24.4%.

[0140] Example 4 Supercritical extract of Cistanche tubulosa - total amount of chrysanthemum glycoside and verbascoside: 37.7%

[0141] The contents of the samples tested above all exceed the contents required in the "Chinese Pharmacopoeia 2020 Edition", which shows that the extraction method of the present invention has a good enrichment effect.

[0142] Example 6

[0143] The preparation of the herbal composition beverage comprises the following steps:

[0144] S1: Weigh each component according to Table 1 below.

[0145] S2: Take the black tea leaves, spread them out and add other ingredients; shake well.

[0146] S3: Vacuum seal and package at 4℃.

[0147] Table 1

[0148] Serial number / mass 1 2 3 4 5 6 7 Polygonatum sibiricum 3 4 5 3 5 6 0 Cistanche tubulosa 3 4 5 3 5 6 0 saffron 2 4 5 2 5 7 0 Phragmites australis (Example 1) 3 4 5 0 0 0 5 Phragmites australis (Example 2) 0 0 0 3 5 0 0 Pueraria root (Example 1) 5 6 8 0 0 0 8 Pueraria root (Example 2) 0 0 0 5 8 0 0 Sterculia lychnophora (Example 1) 3 4 5 0 0 0 6 Plantago ovata (Example 2) 0 0 0 3 5 0 0 black tea 8 9 10 8 10 8 8

[0149] Except for black tea, which is commercially available, all the other ingredients in Table 1 are from the examples; specifically, Phragmites australis, Pueraria lobata, and Sterculia lychnophora are from Examples 1 and 2; the aqueous extracts of Saffron and Polygonatum sibiricum are from Example 3; the supercritical extractions of Saffron, Polygonatum sibiricum, and Cistanche tubulosa are from Example 4; and the mass fractions of Saffron and Polygonatum sibiricum are the sum of the aqueous extract and the supercritical extraction.

[0150] The samples of Example 6 were subjected to the mouse hypoxia fatigue test.

[0151] SPF-grade Kunming mice (4-week-old, male) were selected and divided into 8 groups: 1 group was the control group; the remaining 7 groups were experimental groups; each group had 10 mice, with a body weight of 21.0±1.8 g.

[0152] The animals were acclimated to feeding at an ambient temperature of (25±1)°C and a relative humidity of (70±2)% for 5 days. The experimental group was then gavaged with 4 ml of the test substance solution at a fixed time each day; the control group was gavaged with an equal volume of normal saline.

[0153] The test substance solution is prepared by mixing each sample with 80°C water and then leaving it for 20 minutes; the sample concentration is 50%.

[0154] One hour after the last dose, an hypoxic fatigue test was performed in a 30×50 cm, 60 cm high, 30 cm deep glass water tank. Mice were weighed, and a lead sheet weighing 6% of their body weight was attached to their tails. The time the mice spent resting and swimming, as well as the time they sank and stopped moving, was recorded. The endpoint was when the head was submerged in water for 6 seconds.

[0155] The results are shown in Table 2.

[0156] Table 2

[0157] Serial number Swimming time (min) Elongation (%) 1 36.5±4.3 28.52 2 38.7±3.9 36.27 3 41.6±4.0 46.48 4 34.1±3.3 20.07 5 36.8±3.6 29.58 6 32.9±3.4 15.85 7 33.7±3.8 18.66 control group 28.4±3.6 /

[0158] The experimental results indicate that the herbal beverage composition prepared by the present invention significantly enhances hypoxia tolerance. The effect increases with increasing addition levels (mass ratio of other substances to black tea). Sequence numbers 4 and 5 are comparative experiments comparing partial fermentation with no fermentation at the same dosage as in sequence numbers 1 and 3, respectively. Fermentation results in a significantly improved effect, demonstrating that fermentation of some ingredients is necessary in this invention. Sequence numbers 6 and 7 are experiments comparing sequence number 1 with the default formulation, after equal weight substitution of all ingredients except black tea. The effect decreases with the omission of some ingredients, indicating a synergistic effect between the ingredients in this invention.

[0159] Example 7

[0160] In order to further increase the compliance and palatability of drinkers, the present invention recruited 10 volunteers to conduct a single-blind experiment to compare the taste of two different herbal composition drinks and make scores.

[0161] Wherein sample 1 is the sample No. 1 in Table 1 of Example 6;

[0162] Sample 2 is based on Sample 1, with the following components added in parts by mass:

[0163] 1 portion of five-fingered peach;

[0164] 1 portion of Ophiopogon japonicus;

[0165] 0.2 parts of red lotus seeds;

[0166] 0.5 parts of gorgon fruit;

[0167] 0.2 parts of lily;

[0168] 2 parts winter melon peel;

[0169] 3 parts corn silk;

[0170] 0.5 parts of Ganoderma lucidum;

[0171] 0.3 parts of dandelion;

[0172] 1 small green mandarin orange;

[0173] 0.5 parts of dried tangerine peel;

[0174] 2 servings of barley tea;

[0175] 0.5 parts of Dendrobium flower;

[0176] Eight out of ten volunteers said that Sample 2 tasted mellower and had a more pronounced sweet aftertaste, but they also said that Sample 2 tasted more bitter and had a stronger medicinal flavor than Sample 1.

[0177] The above detailed description is a specific description of one feasible embodiment of the present invention. This embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification that does not depart from the present invention should be included in the scope of the technical solution of the present invention.

Claims

1. A herbal beverage composition capable of clearing meridians and relieving blockage, characterized in that: The following components are included in parts by mass: The preparation method of the reed rhizome, kudzu root and pterostilbene is as follows: the corresponding plant raw materials are fermented with Lactobacillus reuteri, extracted with water, and then freeze-dried; The preparation method of saffron and polygonatum is as follows: firstly extracting with water, freeze-drying the water extract; and extracting the solid matter after water extraction with supercritical carbon dioxide; The Cistanche tubulosa is an extract that has been subjected to supercritical carbon dioxide treatment; The above extracts are mixed with black tea to obtain a herbal plant composition drink capable of clearing meridians and relieving blockages.

2. The herbal composition beverage capable of unblocking meridians and relieving blockage according to claim 1, characterized in that: The preparation method of the reed rhizome, kudzu root and sterculia lychnophora is as follows: fresh reed rhizome, fresh kudzu root and fresh sterculia lychnophora are taken; crushed to at least 80 mesh; and then steamed and sterilized to obtain a substrate; The fermentation parameters of the activated Lactobacillus reuteri after inoculation of the substrate are as follows: temperature 37-40°C; initial pH 6.4±0.2; anaerobic fermentation; fermentation for at least 48 hours; then the fermentation is considered complete if the pH change is ≤0.15 / h; After the fermentation is completed, the product is sterilized and centrifuged to obtain a supernatant and a solid portion. The solid portion is subjected to cell disruption treatment and then water-extracted at a pH of 6-7, at least 50° C., and a solid-liquid ratio of 1: at least 10; the water extract is taken; the water extract and the supernatant are combined and then freeze-dried.

3. The herbal composition beverage capable of unblocking meridians and relieving blockage according to claim 1, characterized in that: The preparation method of the saffron is: The upper part and stigma of saffron are taken and crushed to at least 40 mesh; the mixture is extracted with water at a temperature of 70-80°C and a solid-liquid ratio of 1:15 or more for at least 1 hour; the mixture is filtered while hot to separate the solid and liquid, and the filtrate is freeze-dried; the solid is set aside; The saffron solids are dried to a moisture content of ≤8%; then supercritical carbon dioxide extraction is performed; and after the extraction is complete, the entrainer is removed; The conditions for supercritical extraction are: ≥35MPa, 45-55°C, carbon dioxide flow rate ≥6L / min; and at least 10% ethanol is added as an entrainer.

4. The herbal composition beverage capable of unblocking meridians and relieving blockage according to claim 1, characterized in that: The preparation method of the polygonatum is: Select fresh tubers of Polygonatum sibiricum, steam and dry at low temperature, then crush to at least 40 mesh; extract with water at a temperature of 90-95°C and a solid-liquid ratio of 1:15 or more for at least 1-2 hours; filter while hot to separate the solid and liquid, freeze-dry the filtrate, and set aside the solid; The solid matter of Polygonatum sibiricum is dried to a water content of ≤8%; then subjected to supercritical carbon dioxide extraction; after the extraction is complete, the entrainer is removed; The conditions for supercritical extraction are: ≥35MPa, 45-55°C, carbon dioxide flow rate ≥6L / min; and at least 10% ethanol is added as an entrainer.

5. The herbal composition beverage capable of unblocking meridians and relieving blockage according to claim 1, characterized in that: The preparation method of the Cistanche tubulosa is as follows: Select fresh fleshy stems of Cistanche tubulosa; crush them and pass them through at least 8 mesh; then dry them to a moisture content of ≤8%; then perform supercritical carbon dioxide extraction; and remove the entrainer after the extraction is complete. The conditions for supercritical extraction are: ≥35MPa, 45-55°C, carbon dioxide flow rate ≥6L / min; and at least 10% ethanol is added as an entrainer.

6. The herbal composition beverage capable of unblocking meridians and relieving blockage according to claim 5, characterized in that: The Cistanche tubulosa is enzymatically hydrolyzed with cellulase before drying.

7. The herbal beverage composition capable of unblocking meridians and relieving blockage according to claim 1, characterized in that: Also includes at least one of the following components: Five-fingered ginseng, ophiopogon japonicus, red lotus seeds, gorgon fruit, lily, wax gourd peel, corn silk, ganoderma lucidum, dandelion, small green tangerine peel, tangerine peel, barley tea, dendrobium flower.

8. Use of the herbal plant composition beverage capable of unblocking meridians and relieving blockage according to any one of claims 1 to 7 in the preparation of health products.

9. Use of the herbal plant composition drink capable of unblocking meridians and removing blockages according to claim 8 in the preparation of health products, characterized in that: The health product is a substitute tea.

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