Fermentation device for culturing reuteria reuteri

Abstract

The invention relates to the technical field of fermentation devices, in particular to a fermentation device for culturing reuteria reuteri. In order to solve the problem that foam is difficult to treat efficiently, the invention provides the following technical scheme: the device comprises a fermentation tank and a top cover covering the fermentation tank, and also comprises a water pump fixedly connected to the top of the top cover. By arranging the first rotating assembly, bubbles on the surface layer of a culture solution can be pushed to the periphery of a sixth special-shaped pipe by a bubble pushing plate while bubbles are extracted through an eighth hard pipe and the sixth special-shaped pipe, fermentation liquor and the bubbles on the surface layer are sucked in cooperation with suction force generated by a water pump, and the bubble extraction efficiency is improved; the end of a sixth special-shaped pipe faces upwards, bubbles and liquid below the bubbles can be conveniently extracted together, a bubble pushing plate and the sixth special-shaped pipe can ascend and descend along with fluctuation of the liquid level through a strip-shaped air bag, bubbles on the surface layer of a culture solution can be conveniently extracted in real time, and the bubble treatment efficiency is remarkably improved.

Description

Technical Field

[0001] The present invention relates to the technical field of fermentation devices, in particular to a fermentation device for culturing Bacillus reuteri. Background Art

[0002] Lactobacillus reuteri is a type of lactic acid bacteria found in the intestines of vertebrates and mammals. A Lactobacillus reuteri fermenter is a fermentation facility used to cultivate liquid cultures of Lactobacillus reuteri. Utilizing the principles of biological fermentation, it provides optimal nutrition, pH, temperature, oxygen supply, and pressure for rapid growth, completing a fermentation cycle. The typical process flow includes: cleaning and inspection, culture medium preparation, loading and canning, heating and sterilizing the culture medium, cooling, inoculation of the specialized culture medium, fermentation, sampling, and removal of the culture medium. Lactobacillus reuteri can be cultured in a flexible manner, generally divided into liquid and solid cultures.

[0003] During the fermentation and cultivation of Lactobacillus reuteri in liquid, carbon dioxide, proteins, and polysaccharides are released from the culture medium and the bacteria, accumulating to form a foam mass that hinders gas exchange and bacterial respiration. Prior art fermentation equipment removes foam by blowing bubbles and then sucking them out through a straw positioned above the fermentation liquid. However, this method is inefficient at cleaning bubbles, preventing them from being completely sucked out, resulting in waste of culture liquid. Residual bubbles can also affect bacterial fermentation, leading to reduced bacterial production. Therefore, we propose a fermentation device for Lactobacillus reuteri to address these issues. Summary of the Invention

[0004] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a fermentation device for culturing Bacillus reuteri, which solves the problem of poor bubble cleaning efficiency in the prior art, resulting in waste of culture fluid.

[0005] Therefore, the present invention adopts a fermentation device for culturing Bacillus reuteri, comprising: a fermentation tank and a top cover provided on the fermentation tank, a water pump fixedly connected to the top of the top cover, a filter box fixedly connected to the top of the top cover, bubble storage boxes fixedly connected to both sides of the filter box, a filter screen fixedly connected to the inner wall of the filter box, a hard tube 8 slidably inserted inside the top cover, a plurality of hard tubes 9 fixedly connected to the outer bottom of the hard tube 8, a special-shaped tube 6 fixedly connected to the end of the hard tube 9, a plurality of bubble pushing plates rotatably provided at the bottom of the hard tube 8, strip-shaped air bags fixedly connected to both sides of the bubble pushing plates, a limiting tube rotatably provided at the top of the hard tube 8, a pushing component 2 provided above the limiting tube for pushing the foam on the filter screen, and a rotating component 1 provided in the hard tube 8 for driving the bubble pushing plate; the rotating component 1 includes a transmission rod 5 rotatably provided in the hard tube 8, a plurality of cross bars 1 rotatably provided on the transmission rod 5, and an impeller fixedly provided on the transmission rod 5, the ends of the plurality of cross bars 1 being fixedly connected to the inner wall of the hard tube 8, and the bottom of the transmission rod 5 being fixedly connected to the plurality of bubble pushing plates.

[0006] Furthermore, a rotating joint is rotatably provided on the top of the hard tube eight, a rotating cover is fixedly provided on the top of the rotating joint, a limiting rod is fixedly provided on the top of the rotating cover, and the lower end of the rotating cover is fixedly connected to the top of the transmission rod five.

[0007] Furthermore, a limiting groove is provided at the lower end of the limiting tube, and the limiting rod is inserted into the limiting groove.

[0008] Furthermore, the outer surface of the limiting tube is sleeved with a bearing 1, and the outer surface of the bearing 1 is fixedly connected to the top of the top cover through a support frame.

[0009] Furthermore, the transmission rod five passes through the bottom of the hard tube eight, and the transmission rod five is rotatably connected to the hard tube eight.

[0010] Furthermore, two groups of symmetrically arranged side holes are opened between the filter box and the foam storage box, and the side holes are connected to the foam storage box, and the filter screen is located between the two side holes.

[0011] Furthermore, the pushing component 2 includes a push plate 7 movably fitted on the top of the filter screen and a push rod 6 movably passing through the filter box, the end of the push rod 6 is fixedly connected to the side of the push plate 7, one end of the push rod 6 is fixedly connected to the fixed frame, one end of the fixed frame is rotatably connected to the roller, the top of the limiting tube is fixedly connected to the special-shaped plate, the side surface of the roller is in contact with the side surface of the special-shaped plate, the surface of the push rod 6 is provided with a spring, and the two ends of the spring are respectively clamped on the fixed frame and the filter box.

[0012] Furthermore, the hard tube eight is connected to the input end one of the water pump through the hose one, the output end of the water pump is connected to the top of the filter box through the hose two, and the bottom of the filter box is connected to the fermentation tank through the hose three through the top cover.

[0013] Furthermore, one end of the special-shaped tube six is ​​connected to the hard tube nine, and the hard tube eight is connected to the soft tube one.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. By providing the rotating component 1, while extracting bubbles through the hard tube 8 and the special-shaped tube 6, the bubble pushing plate can push the bubbles on the surface of the culture solution to the vicinity of the special-shaped tube 6, and cooperate with the suction force generated by the water pump to absorb the fermentation liquid and bubbles on the surface, thereby improving the efficiency of bubble extraction. By pointing the end of the special-shaped tube 6 upward, it is convenient to extract the bubbles and the liquid below the bubbles together. The strip-shaped air bag can make the bubble pushing plate and the special-shaped tube 6 rise and fall with the fluctuation of the liquid level, facilitating the real-time extraction of bubbles on the surface of the culture solution, and significantly improving the efficiency of bubble processing.

[0016] Second, by setting up the pushing component 2, the bubbles remaining after the filter screen filters the bubble-liquid mixture can be scraped into the bubble storage boxes on both sides of the filter screen to prevent the bubbles from affecting the filtration efficiency of the filter screen. After the liquid passes through the filter screen, it flows back into the fermentation tank, realizing the precise extraction of bubbles, improving the utilization rate of the culture solution, being beneficial to the gas exchange and bacterial respiration on the surface of the culture solution, eliminating the interference of bubbles on bacterial sampling and detection, and being beneficial to increasing the yield of Lactobacillus reuteri. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort.

[0018] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0019] Figure 2 Schematic diagram of the three-dimensional structure of the hard tube eight and the hard tube nine of the present invention;

[0020] Figure 3 Schematic diagram of the three-dimensional cross-section of the hard tube 8 of the present invention;

[0021] Figure 4 Schematic diagram of the explosion structure of the limiting rod and the limiting tube of the present invention;

[0022] Figure 5 Schematic diagram of the three-dimensional structure of the transmission rod 5 and the bubble pushing plate of the present invention;

[0023] Figure 6 Schematic diagram of the cross-section three-dimensional structure of the filter box and the foam storage box of the present invention;

[0024] Figure 7 Schematic diagram of the three-dimensional structure of the transmission rod 5 and the impeller of the present invention;

[0025] Figure 8 It is an enlarged structural schematic diagram of part A of the present invention.

[0026] Figure numerals: 1, fermentation tank; 2, top cover; 3, water pump; 4, hard tube eight; 5, rotary joint; 6, limiting tube; 7, limiting rod; 8, rotating component one; 9, pushing component two; 10, limiting groove; 11, filter box; 12, bubble storage box; 13, filter screen; 14, side hole; 15, bubble push plate; 16, strip air bag; 17, hard tube nine; 18, special-shaped tube six; 19, support frame; 20, rotating cover; 81, transmission rod five; 82, impeller; 83, cross bar one; 91, special-shaped plate; 92, roller; 93, fixed frame; 94, push rod six; 95, push plate seven; 96, spring. DETAILED DESCRIPTION

[0027] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0028] The present invention will be further described below with reference to the embodiments.

[0029] Example 1, refer to the attached Figure 1-8In order to solve the problem of poor bubble cleaning efficiency and waste of culture fluid in the prior art, the present invention discloses a fermentation device for culturing Bacillus reuteri, comprising: a fermentation tank 1, the fermentation tank 1 is used to ferment Bacillus reuteri, and a top cover 2 provided on the fermentation tank 1, the top cover 2 is used for sealing, and further comprising a water pump 3 fixedly connected to the top of the top cover 2, the water pump 3 is used to extract liquid and bubbles from the surface of the culture fluid, a filter box 11 fixedly connected to the top of the top cover 2, the filter box 11 is used to filter bubbles and allow the liquid to flow back into the fermentation tank 1, and bubble storage boxes 12 fixedly connected to both sides of the filter box 11, the bubble storage boxes 12 are used to store filtered bubbles. The bubbles after the filter box 11 are fixedly connected to the filter screen 13 on the inner wall of the filter box 11, the filter screen 13 is used to filter the bubbles, the hard tube 84 is slidably inserted into the inside of the top cover 2, the hard tube 84 is used to transport the bubble-liquid mixture, a plurality of hard tubes 9 17 are fixedly connected to the bottom of the outer side of the hard tube 84, the hard tube 9 17 is used to transport the bubble-liquid mixture, a special-shaped tube 6 18 is fixedly connected to the end of the hard tube 9 17, the special-shaped tube 6 18 is used to absorb the bubble-liquid mixture, a plurality of bubble pushing plates 15 arranged at the bottom of the hard tube 84 are rotated, the bubble pushing plates 15 are used to push the bubbles on the surface of the culture solution to make it flow to the special-shaped tube 6 18, the strip air bags 16 are fixedly connected to both sides of the bubble pushing plates 15, and the strip air bags The capsule 16 is used to make the bubble pushing plate 15 rotate the limiting tube 6 set on the top of the hard tube 84, so that the hard tube 9 17 and the hard tube 84 float, and the top cover 2 limits the hard tube 84. A pushing component 29 is set above the limiting tube 6 to push the foam on the filter 13. The pushing component 29 is used to push the foam on the filter 13 to move to the bubble storage boxes 12 on both sides. A rotating component 18 is set in the hard tube 84 to drive the bubble pushing plate 15. The rotating component 18 is used to rotate the bubble pushing plate 15 and push the liquid bubble mixture to flow toward the special-shaped tube 6 18. The limiting tube 6 is used to limit the up and down movement of the hard tube 84 and can transmit the pushing component 29; the rotating component 18 includes There is a transmission rod 5 81 rotatably set in the hard tube 84, and the transmission rod 5 81 is used for transmission, multiple cross bars 83 rotatably set on the transmission rod 5 81 and an impeller 82 fixedly set on the transmission rod 5 81, multiple cross bars 83 are used to limit the rotation of the transmission rod 5 81 and the impeller 82 fixedly set on the transmission rod 5 81 through bearings, and the impeller 82 is used to rotate through the force of liquid flow, so that the bubble pushing plate 15 and the limiting rod 7 rotate, the ends of the multiple cross bars 83 are fixedly connected to the inner wall of the hard tube 84, and the bottom of the transmission rod 5 81 is fixedly connected to the multiple bubble pushing plates 15. The fixed connection method facilitates the transmission rod 5 81 and the multiple bubble pushing plates 15 to rotate together.

[0030] The top of the hard tube eight 4 is rotatably provided with a rotating joint 5, which facilitates the rotation of the rotating cover 20. The top of the rotating joint 5 is fixedly provided with a rotating cover 20. The top of the rotating joint 5 is fixedly provided with a rotating cover 20 to drive the limit rod 7 to rotate. The top of the rotating cover 20 is fixedly provided with a limit rod 7. The top of the rotating cover 20 is fixedly provided with a limit rod 7 to move up and down in the limit tube 6, driving the limit tube 6 to rotate. The lower end of the rotating cover 20 is fixedly connected to the top of the transmission rod five 81, so as to enable the transmission rod five 81 to drive the rotating cover 20 to rotate.

[0031] The lower end of the limiting tube 6 is provided with a limiting groove 10, which is used to limit the limiting tube 6 and the limiting rod 7. The limiting rod 7 is inserted into the limiting groove 10. An insertion method is adopted.

[0032] The outer surface of the limiting tube 6 is sleeved with a bearing 1, which facilitates the rotation of the limiting tube 6, and the outer surface of the bearing 1 is fixedly connected to the top of the top cover 2 through a support frame 19, which is used to support the bearing 1 on the limiting tube 6.

[0033] The transmission rod 5 81 passes through the bottom of the hard tube 84 to achieve a sealing effect and facilitate the rotation of the transmission rod 5 81. The transmission rod 5 81 is rotatably connected to the hard tube 84, and the rotation of the transmission rod 5 81 also plays a sealing role.

[0034] Two groups of symmetrically arranged side holes 14 are provided between the filter box 11 and the foam storage box 12, so that bubbles can flow into the foam storage box 12 through the side holes 14, and the foam can be pushed out through the side holes 14 by using a push plate 7 95. The side holes 14 are connected to the foam storage box 12, and the filter screen 13 is located between the two side holes 14, so that the foam can be pushed into the foam storage box 12 through the side holes 14 by using a push plate 7 95.

[0035] The pushing component 2 9 includes a push plate 7 95 that is movably attached to the top of the filter screen 13. The push plate 7 95 is used to push the foam. The filter screen 13 is used to filter out the foam, and a push rod 6 94 that is movable through the filter box 11. The push rod 6 94 is limited by the filter box 11. The end of the push rod 6 94 is fixedly connected to the side of the push plate 7 95 to facilitate pushing the push plate 7 95. One end of the push rod 6 94 is fixedly connected to a fixed frame 93 for fixing the roller 92 so that it rotates. One end of the fixed frame 93 is rotatably connected to the roller 92. The top of the limiting tube 6 is fixedly connected to a special-shaped plate 91. The special-shaped plate 91 can make the push rod 6 94 move back and forth, and the side surface of the roller 92 can be abutted against the side surface of the special-shaped plate 91, which facilitates the contact transmission between the special-shaped plate 91 and the roller 92. The side surface of the roller 92 is in contact with the side surface of the special-shaped plate 91, and the roller 92 is convenient for reducing the resistance between the push rod 6 94 and the special-shaped plate 91. The surface of the push rod 6 94 is provided with a spring 96, which gives the push rod 6 94 a pushing force, which cooperates with the force transmitted by the special-shaped plate 91 to facilitate the reciprocating movement of the push rod 6 94, and the two ends of the spring 96 are respectively clamped on the fixed frame 93 and the filter box 11, which is convenient for fixing the spring 96.

[0036] The hard tube 84 is connected to the input end 1 of the water pump 3 through the hose 1, the output end of the water pump 3 is connected to the top of the filter box 11 through the hose 2, and the bottom of the filter box 11 is connected to the fermentation tank 1 through the hose 3 through the top cover 2, forming a channel for transmitting liquid bubbles.

[0037] One end of the special-shaped tube six 18 is connected to the hard tube nine 17. The special-shaped tube six 18, because the fine mouth of the special-shaped tube six 18 is facing upward, is convenient for absorbing the surface liquid of the culture solution while absorbing bubbles. The hard tube nine 17 is used to absorb bubbles and liquid. The hard tube eight 4 and the soft tube one are connected to form a channel for transmitting liquid and bubbles.

[0038] Working principle: Start the water pump 3, so that the foam-liquid mixture on the surface of the culture liquid in the fermentation tank 1 is extracted through the special-shaped pipe 6 18. The foam-liquid mixture enters the filter box 11 from the special-shaped pipe 18 through the hard pipe 9 17, the hard pipe 8 4, the hose 1, the water pump 3 and the hose 2. After being filtered by the filter 13, the foam enters the foam storage box 12. The liquid flows back from the filter box 11 to the fermentation tank 1. The liquid flows back to the fermentation tank 1 through the bottom of the filter box 11 and the hose 3. In this process:

[0039] S1. When the foam-liquid mixture enters the hard tube 84, the pressure of the foam-liquid mixture drives the impeller 82 to rotate, thereby driving the transmission rod 5 81 to rotate, causing the bubble pushing plate 15 to rotate as well, pushing the foam-liquid mixture on the surface of the culture liquid toward the special-shaped tube 6 18. As the foam-liquid mixture is extracted, under the action of the strip-shaped airbag 16, the bubble pushing plate 15 and the special-shaped tube 6 18 also rise and fall with the rise and fall of the liquid level, driving the limit rod 7 to move up and down in the limit tube 6. As the transmission rod 5 81 rotates, it also drives the rotating cover 20 on the rotary joint 5 to rotate, thereby driving the limit rod 7, the limit tube 6 and the special-shaped plate 91 to rotate;

[0040] S2. The special-shaped plate 91 rotates with the center of the limiting tube 6 as the center, thereby driving the roller 92 to rotate. Under the action of the spring 96, the push rod 6 94 and the push plate 7 95 move back and forth, so that the push plate 7 95 pushes the foam in the filter screen 13 back and forth to both sides of the filter screen 13, so that the foam enters the foam storage box 12, and the liquid flows down along the filter screen 13 into the fermentation tank 1.

[0041] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A fermentation device for culturing Bacillus reuteri, comprising a fermentation tank (1) and a top cover (2) provided on the fermentation tank (1), characterized in that: It also includes a water pump (3) fixedly connected to the top of the top cover (2), a filter box (11) fixedly connected to the top of the top cover (2), a bubble storage box (12) fixedly connected to both sides of the filter box (11), a filter screen (13) fixedly connected to the inner wall of the filter box (11), a hard tube eight (4) slidably inserted into the interior of the top cover (2), a plurality of hard tube nines (17) fixedly connected to the outer bottom of the hard tube eight (4), a special-shaped tube six (18) fixedly connected to the end of the hard tube nine (17), a plurality of bubble pushing plates (15) rotatably arranged at the bottom of the hard tube eight (4), a strip-shaped air bag (16) fixedly connected to both sides of the bubble pushing plate (15), a limiting tube (6) rotatably arranged at the top of the hard tube eight (4), a pushing component two (9) arranged above the limiting tube (6) for pushing the foam on the filter screen (13), and a rotating component one (8) arranged in the hard tube eight (4) for driving the bubble pushing plate (15); The rotating assembly (8) includes a transmission rod (81) rotatably arranged in the hard tube (4), a plurality of cross rods (83) rotatably arranged on the transmission rod (81), and an impeller (82) fixedly arranged on the transmission rod (81). The ends of the plurality of cross rods (83) are fixedly connected to the inner wall of the hard tube (4), and the bottom of the transmission rod (81) is fixedly connected to the plurality of bubble pushing plates (15).

2. A fermentation device for culturing Bacillus reuteri according to claim 1, characterized in that: A rotary joint (5) is rotatably provided on the top of the hard tube eight (4), a rotary cover (20) is fixedly provided on the top of the rotary joint (5), a limiting rod (7) is fixedly provided on the top of the rotary cover (20), and the lower end of the rotary cover (20) is fixedly connected to the top of the transmission rod five (81).

3. A fermentation device for culturing Bacillus reuteri according to claim 2, characterized in that: A limiting groove (10) is provided at the lower end of the limiting tube (6), and the limiting rod (7) is inserted into the limiting groove (10).

4. The fermentation device for culturing Bacillus reuteri according to claim 3, characterized in that: The outer surface of the limiting tube (6) is sleeved with a bearing 1, and the outer surface of the bearing 1 is fixedly connected to the top of the top cover (2) via a support frame (19).

5. The fermentation device for culturing Bacillus reuteri according to claim 2, characterized in that: The transmission rod five (81) passes through the bottom of the hard tube eight (4), and the transmission rod five (81) is rotatably connected to the hard tube eight (4).

6. The fermentation device for culturing Bacillus reuteri according to claim 1, characterized in that: Two groups of symmetrically arranged side holes (14) are provided between the filter box (11) and the foam storage box (12), and the side holes (14) are connected to the foam storage box (12), and the filter screen (13) is located between the two side holes (14).

7. The fermentation device for culturing Bacillus reuteri according to claim 6, characterized in that: The pushing component 2 (9) includes a push plate 7 (95) movably attached to the top of the filter screen (13) and a push rod 6 (94) movably passing through the filter box (11), the end of the push rod 6 (94) is fixedly connected to the side of the push plate 7 (95), one end of the push rod 6 (94) is fixedly connected to the fixed frame (93), one end of the fixed frame (93) is rotatably connected to the roller (92), the top of the limiting tube (6) is fixedly connected to the special-shaped plate (91), the side surface of the roller (92) is in contact with the side surface of the special-shaped plate (91), the surface of the push rod 6 (94) is provided with a spring (96), and the two ends of the spring (96) are respectively clamped on the fixed frame (93) and the filter box (11).

8. The fermentation device for culturing Bacillus reuteri according to claim 6, characterized in that: The hard tube eight (4) is connected to the input end one of the water pump (3) through the hose one, the output end of the water pump (3) is connected to the top of the filter box (11) through the hose two, and the bottom of the filter box (11) is connected to the fermentation tank (1) through the hose three through the top cover (2).

9. The fermentation device for culturing Bacillus reuteri according to claim 8, characterized in that: One end of the special-shaped tube six (18) is connected to the hard tube nine (17), and the hard tube eight (4) is connected to the soft tube one.

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