Antibacterial oil-control composition and application thereof

Abstract

The invention belongs to the field of daily chemicals, and discloses an antibacterial oil-control composition which is composed of lauryl imino dipropylene glycol and terpineol. The weight ratio of the lauryl imino dipropylene glycol to the terpineol is (0.01 to 0.36) to (0.09 to 0.04). The composition has double effects of resisting bacteria and controlling oil. Meanwhile, the invention also provides application of the composition.

Description

Technical Field

[0001] The present invention relates to the field of daily chemicals, and in particular to an antibacterial oil-control composition and applications thereof. Background Art

[0002] The patent application with publication number KR102689237B1, which is a patent application for a preservative composition with improved preservative power containing lauryl aminodipropylene glycol, discloses that lauryl aminodipropylene glycol has an antibacterial effect.

[0003] The disclosed antibacterial targets are Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, Enterobacter cloacae, Enterobacter gergovina, Burkholderia cepacia, Pseudomonas mendocina, Pseudomonas stutzeri, Pseudomonas fluorescens, Pseudomonas putida), Alcaligenes faecalis, Ochrobacterium tritici, Rhizobium, Rhizobium radiorhizobium, common aquatic monosaccharides, containing at least one kuyae and Thaueramecernichensis selected from Spingobium yanoi, fungi including Candida albicans, Candida tropicalis, Candida asteroides, Candida glabrata, Candida krusei, Candida guillimondi, Candida viswanathi, Candida lusitaniae, Yarrowia lipolytica, Cryptococcus neoformans, Cryptococcus gattii, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, Aspergillus brasiliensis;

[0004] This proposal does not disclose the antibacterial effect of lauryl aminodipropylene glycol on Malassezia. Summary of the Invention

[0005] The object of the present invention is to provide an antibacterial and oil-controlling composition, which has both antibacterial and oil-controlling effects.

[0006] At the same time, the invention also provides application of the composition.

[0007] To achieve the above objectives, the present application discloses an antibacterial oil-control composition, which is composed of lauryl iminodipropylene glycol and terpineol;

[0008] The weight ratio of the lauryl iminodipropylene glycol and terpineol is 0.01:0.36 to 0.09:0.04.

[0009] The invention also discloses the use of the combination in preparing an antibacterial agent.

[0010] In the above-mentioned use, the antibacterial agent is used to inhibit the proliferation of Malassezia.

[0011] In addition, the present invention also discloses the use of the combination for preparing cosmetics.

[0012] In the above-mentioned use, the function of the cosmetic in skin care is to control skin oil and / or inhibit the proliferation of Malassezia on the skin.

[0013] Finally, the present invention also discloses a cosmetic containing 0.01 to 1 wt % of the above composition.

[0014] The present invention has the following advantages and effects compared to the prior art:

[0015] The present invention uses different proportions of lauryl iminodipropylene glycol and terpineol, as well as the Malassezia inhibition test when the two are used alone, which clearly shows that the composition of the present invention has good antibacterial synergy; at the same time, it can be seen from the oil control test that the application formula of the present invention has a good oil control effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is the antibacterial graph of Malassezia of sample 1 of the present invention;

[0017] Figure 2 This is the antibacterial graph of Malassezia of sample 2 of the present invention;

[0018] Figure 3 This is the antibacterial graph of Malassezia of sample 3 of the present invention;

[0019] Figure 4 This is the antibacterial graph of Malassezia of sample 4 of the present invention;

[0020] Figure 5 This is the antibacterial graph of Malassezia of sample 5 of the present invention;

[0021] Figure 6 This is the antibacterial graph of Malassezia of sample 6 of the present invention;

[0022] Figure 7 This is a bar graph showing the oil control effect of Sample 7 of the present invention. DETAILED DESCRIPTION

[0023] The present invention will be described clearly and completely below in conjunction with the examples of the present invention. In the description of the present invention, it should be noted that, where specific conditions are not specified in the examples, conventional conditions or conditions recommended by the manufacturer were used. Reagents or instruments used where the manufacturer is not specified are conventional products that can be purchased commercially.

[0024] Sample preparation

[0025] The lauryl iminodipropylene glycol used in the sample of the present invention is a lauryl iminodipropylene glycol aqueous solution with a concentration of 25 wt %; and the terpineol is terpineol with a net content of 100%.

[0026] The formula of the sample of the present invention is shown in Table 1;

[0027] Table 1 Recipe Unit: g

[0028]

[0029]

[0030] Performance testing

[0031] 1. Malassezia antibacterial test

[0032] The above 6 samples were sent to Guangzhou Huamiao Testing Service Co., Ltd. for testing, and the test report numbers were 2505WH028-01A sample 1, 2505WH028-02A sample 2, 2505WH028-03A sample 3, 2505WH028-04A sample 4, 2505WH028-05A sample 5, and 2506WH015-01A sample 6.

[0033] The detection method is:

[0034] Experimental materials and methods

[0035] 1.1 Instruments and Equipment

[0036] Biochemical incubator

[0037] Vertical high pressure steam sterilizer

[0038] 1.2 Reagents

[0039] Phosphate buffer

[0040] Pityrosporum culture medium

[0041] Malassezia

[0042] 1.3 Test methods

[0043] (1) Treatment of control and test samples

[0044] Sample: stock solution

[0045] Blank control: phosphate buffer

[0046] Control sample group: Malassezia suspension + phosphate buffer

[0047] (2) Test operation steps

[0048] Take a Malassezia suspension and add it to each test sample and control sample, mixing thoroughly. Start timing. When the designated exposure time has elapsed, make an appropriate dilution. Take two to three dilutions and place the test sample and control sample onto two separate plates. Pityrosporum culture medium is then added and mixed thoroughly. After solidification, flip the plates over and incubate in an incubator for 5 to 10 days. Count the viable bacteria.

[0049] (3) Calculation formula

[0050] Inhibition rate = (AB) / A*100%;

[0051] Wherein, A-average colony count of control samples;

[0052] B-average colony count of the tested samples;

[0053] (4) Data Analysis

[0054] Evaluation criteria: When the inhibition rate is not less than 90%, it indicates that the product has a strong antibacterial effect under this condition; when the inhibition rate is not less than 50% and less than 90%, it indicates that the product has an antibacterial effect under this condition; and when it is less than 50%, it indicates that the product has no antibacterial effect under this condition.

[0055] The test results are shown in Table 2 and Figures 1 to 6 :

[0056] Table 2 Antibacterial test results

[0057]

[0058]

[0059] Result analysis:

[0060] The above test results show that: the 0.4% terpineol solution (sample 5) has no antibacterial ability; the solution with a net content of 0.01% lauryl iminodipropylene glycol (sample 6) has slight antibacterial ability; the solution with a net content of 0.1% lauryl iminodipropylene glycol (sample 6) has very good antibacterial ability;

[0061] From the test results of samples 1 to 3, as long as the net content of lauryl iminodipropylene glycol is not less than 0.01% and terpineol is present, a strong antibacterial effect can be achieved.

[0062] Based on the test data of sample 2 and the test results of samples 5 and 6, it can be considered that lauryl iminodipropylene glycol and terpineol have strong antibacterial synergy.

[0063] 2. Oil control test

[0064] Sample 7 was sent to Guangzhou Huamiao Testing Services Co., Ltd. for testing, and the test report number was 2506WH015-02A Sample 7;

[0065] The detection method is:

[0066] 1. Purpose and Principle of the Test

[0067] Oily skin has a highly active sebaceous gland secretion function, resulting in a greasy feeling on the face, difficulty in cleaning, and an unsightly sheen. It is also prone to skin diseases such as acne vulgaris and seborrheic dermatitis. Given the relationship between 5α-reductase and sebaceous glands, the inhibitory effect of test samples on 5α-reductase is often used to reflect the sebum regulation effect and thus characterize the oil control efficacy of the test sample.

[0068] This test refers to the laboratory method (HMC-WI-031 5α-reductase inhibition rate) and compares the 5α-reductase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and the difference is significant, the test sample can be considered to have oil control effect.

[0069] This test method is an in vitro method suitable for evaluating cosmetic products that claim to achieve oil control by inhibiting 5α-reductase.

[0070] 2. Test indicators

[0071] If the 5α-reductase inhibition rate of the sample is greater than 0 and the change in testosterone concentration is significantly different from that of the negative control, the test sample can be considered to have oil-control efficacy.

[0072] 3. Experimental Materials and Methods

[0073] 3.1 Instruments and Equipment

[0074] BSA224S Analytical Balance

[0075] Agilent 1220 HPLC

[0076] 3.2 Reagents

[0077] testosterone, 98%;

[0078] NADPH, 99%;

[0079] Methanol, HPLC grade;

[0080] Buffer: PBS (pH=5.5);

[0081] 3.3 Test methods

[0082] (1) Treatment of control and test samples

[0083] Sample group: diluted with buffer to a sample test concentration of 1%;

[0084] Positive control (finasteride, purity ≥98%): Dissolve in methanol first, then dilute with buffer to a test concentration of 400 nM;

[0085] Negative control: PBS buffer

[0086] (2) Test operation steps

[0087] A sample group, a positive control group, and a negative control group were set up respectively, and different reagent solutions were added to each group and shaken well. Each group was subjected to HPLC analysis to determine the change in testosterone concentration in each tube.

[0088] (3) Calculation formula

[0089] 5α-reductase inhibition rate (%) = (change in testosterone concentration in the negative control group - change in testosterone concentration in the sample group) * 100 / change in testosterone concentration in the negative control group.

[0090] Experimental results: Laboratory method (HMC-WI-031 5α-reductase inhibition rate) was used to test the oil control effect of the sample. The test results showed that the positive control 5α-reductase inhibition rate was >50%, indicating that the reaction system was effective. When the sample test concentration was 1%, the 5α-reductase inhibition rate was 70.091%, and the change in testosterone concentration was significantly different from that of the negative control (P<0.01), indicating that the sample has oil control effect. For details, please refer to Figure 7 .

[0091] Application formula

[0092] The present invention can be applied to wash-off products such as shower gel and shampoo; the specific formula is as shown in Table 3 below:

[0093] Table 3 Body wash product formula

[0094]

[0095]

[0096] The preparation method is:

[0097] 1. Add the raw materials of phase A into the water phase pot in sequence, stir and heat to 80-85℃, keep warm and stir until completely dissolved.

[0098] 2. When cooled to 43-45℃, add the ingredients of phase B in sequence and stir evenly.

[0099] 3. Take samples to test various indicators, and filter out the materials after they are qualified.

[0100] The formula of the shampoo can be seen in Table 4;

[0101] Table 4 Shampoo formula

[0102]

[0103]

[0104] The preparation method is:

[0105] 1. Weigh phase A and stir evenly, then add pre-dispersed phases B and C respectively. Place the beaker in a water bath at 85-90°C, start stirring (speed 120-150 rpm), and stir for 15-20 minutes to ensure that the raw materials are completely dissolved;

[0106] 2. Add phase D and stir for 15 minutes (120-150 rpm);

[0107] 3. Add phase E and stir for 15 minutes until completely dissolved;

[0108] 4. Add phase F, stir evenly, add water, and stir at room temperature (speed 120-150 rpm);

[0109] 5. Cool down to 45℃, add phase G, stir evenly, and then discharge.

[0110] The above-mentioned shower gel was used as a sample, and a blank sample was prepared at the same time; the blank sample formula was substantially the same as that in Table 2, and the blank sample did not contain lauryl iminodipropylene glycol and 4-terpineol;

[0111] 20 volunteers were recruited, with ages ranging from 23 to 45 years old, half male and half female; the test method was: repeated open smear test.

[0112] Test site: 3cm×3cm area on the flexor side of the forearm; Operation method: Apply twice a day (0.050g±0.005 / time) for 7 consecutive days; Assess the skin condition before each application. If the skin shows irritation before the test, stop the experiment.

[0113] Skin reactions were observed on the 8th, 9th and 10th days respectively. No adverse skin reactions were observed in the test samples and the blank samples, indicating that the composition of the present invention was non-irritating.

Claims

1. An antibacterial oil-control composition, characterized in that: Composed of lauryl iminodipropylene glycol and terpineol; The weight ratio of the lauryl iminodipropylene glycol and terpineol is 0.01:0.36 to 0.09:0.

04.

2. Use of the combination according to claim 1 in preparing an antibacterial agent.

3. The use according to claim 2, characterized in that The antibacterial agent is used to inhibit the proliferation of Malassezia.

4. Use of the combination according to claim 1 in preparing cosmetics.

5. The use according to claim 4, characterized in that The cosmetic has the skin care function of controlling oiliness of the skin and / or inhibiting the proliferation of Malassezia on the skin.

6. A cosmetic, characterized in that: Contains 0.01 to 1 wt% of the composition according to claim 1.

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