Pipe cap structure, reaction pipe and reagent card box

Abstract

The embodiment of the invention provides a tube cap structure, a reaction tube and a reagent card box, and relates to the technical field of nucleic acid detection. The pipe cap structure comprises a pipe cap body and a puncturable sealing piece, the pipe cap body is used for being installed at the opening, and the puncturable sealing piece is arranged on the pipe cap body and used for sealing the reaction cavity. After the tube cap body and the opening of the reaction tube body are installed, the puncturable sealing element can seal the reaction cavity of the reaction tube body, when nucleic acid detection is carried out, only the connecting tube of the reagent card box needs to be sleeved with the reaction tube body, then the puncturable sealing element is punctured through the fluid supply tip, an extracted nucleic acid sample and a reaction reagent are injected into the reaction cavity, and then the reaction tube body is sealed. Redissolving with a freeze-drying reagent in the reaction cavity and then reacting; the whole operation process is simple, and the pipe cap structure does not need to be removed. The pipe cap structure can avoid pollution of a reaction system, is convenient to operate, and improves the working efficiency.

Description

Technical Field

[0001] The present invention relates to the field of nucleic acid detection technology, and in particular to a tube cap structure, a reaction tube and a reagent cartridge. Background Art

[0002] Fluorescence quantitative PCR is a leading technology in clinical molecular testing. It is more sensitive, specific, quantitatively accurate, has a shorter window period, and is faster than traditional methods such as ELISA. Currently, this method typically uses liquid reagents that must be shipped and stored under a cold chain, resulting in high packaging, logistics, and storage costs. It can also be lost due to cold chain transport failures, pipetting errors, repeated freeze-thaw cycles, and contamination of aerosolized amplification products. Therefore, fluorescence quantitative PCR reagents are often manufactured as dry reagents that are stable at room temperature.

[0003] When conducting fully automatic nucleic acid testing, the extracted nucleic acid sample and the reconstitution reagent need to be transferred to a reaction tube containing a freeze-dried reagent in order to perform nucleic acid amplification testing. Since the freeze-dried reagent needs to be stored in a sealed state, the user generally needs to open the reaction tube, add the sample and reaction reagent, and then perform amplification testing. However, this method will bring the risk of contamination to the reaction system, and it is inconvenient for users to use and has low efficiency. Especially in molecular POC testing, in order to improve the convenience of testing, molecular POC detection cartridges generally have the characteristics of "sample in, result out", and are integrated with the structures required for sample lysis, nucleic acid release, purification, and amplification. Among them, amplification generally uses a reaction tube connected to the cartridge body as a reaction chamber. When the reaction tube is used as an amplification chamber and a donor of the reagents required for amplification, the above problems also restrict the convenience and accuracy of molecular POC testing. Summary of the Invention

[0004] The objects of the present invention include, for example, providing a tube cap structure, a reaction tube and a reagent cartridge, which can avoid contamination of the reaction system, are convenient to operate and improve work efficiency.

[0005] The embodiments of the present invention can be implemented as follows:

[0006] In a first aspect, the present invention provides a tube cap structure for adapting to a reaction tube body, wherein the reaction tube body has a reaction chamber and an opening, and the tube cap structure comprises:

[0007] a pipe cap body, the pipe cap body being adapted to be mounted on the opening; and

[0008] A puncturable seal is provided on the tube cap body for sealing the reaction chamber.

[0009] In an optional embodiment, the tube cap body has a channel for the fluid supply tip of the reagent cartridge to extend into, and the pierceable seal is provided on the channel.

[0010] In an optional embodiment, the pipe cap body is provided with a vent, which passes through the inner wall and the outer wall of the pipe cap body and is connected to the pipeline.

[0011] In an optional embodiment, the tube cap body further has a first port and a second port, and an outer wall of the tube cap body is provided with a first annular rib, the first annular rib is located between the first port and the second port and is arranged around the vent;

[0012] The first annular rib is used to separate the vent together with the inner wall of the reaction tube body when abutting against the opening, so that a part of the vent is communicated with the reaction chamber and the other part is communicated with the outside.

[0013] In an optional embodiment, the first port, the pipeline and the second port are connected in sequence, the first port is used to match the opening, the second port is used to extend into the reaction tube body, and the puncturable seal is provided at the second port to seal the reaction chamber.

[0014] In an optional embodiment, the outer side wall of the tube cap body is further provided with a second annular rib, and the second annular rib is located at the first port to abut against the opening.

[0015] In an optional embodiment, the first port, the pipe and the second port are connected in sequence, the first port is used to match the opening, the second port is used to extend into the reaction tube body, and the puncturable seal is provided at the first port to seal the reaction chamber.

[0016] In a second aspect, the present invention provides a reaction tube comprising a reaction tube body and a tube cap structure as described in any one of the aforementioned embodiments, wherein the reaction tube body has a reaction chamber and an opening, and the tube cap body is installed in the opening so that the puncturable seal seals the reaction chamber; the reaction tube body is used to load a connecting tube of a reagent cartridge so that the fluid supply tip of the reagent cartridge punctures the puncturable seal.

[0017] In an optional embodiment, the reaction tube body includes a first tube body and a second tube body that are connected to each other, the first tube body is used to install the connecting tube, the second tube body has the reaction chamber, and the first tube body is sleeved on the tube cap body so that the tube cap body abuts against the inner wall of the second tube body and the puncturable seal seals the reaction chamber.

[0018] In an optional embodiment, a third annular rib is provided on the outer side wall of the first tube body, and the third annular rib is located at an end of the first tube body away from the second tube body.

[0019] In an optional embodiment, the outer side wall of the first tube body is further provided with a fourth annular rib, and the fourth annular rib and the third annular rib are spaced apart from each other along the axial direction of the first tube body.

[0020] In an optional embodiment, the diameter of the second tube body gradually decreases in a direction away from the first tube body.

[0021] In an optional embodiment, a first annular groove is formed on the inner side wall of the first tube body, and the first annular groove is used to install the first annular rib of the tube cap structure.

[0022] In a third aspect, the present invention provides a reagent cartridge comprising a connecting tube, a fluid supply tip, and a reaction tube according to any one of the preceding embodiments, wherein a portion of the fluid supply tip is disposed within the connecting tube, another portion of the fluid supply tip extends out of the connecting tube, and the fluid supply tip has a flow channel for circulating a nucleic acid sample and a reaction reagent.

[0023] The connecting tube is sleeved on the reaction tube body, and the fluid supply tip is used to pierce the pierceable seal to allow the nucleic acid sample and the reaction reagent to flow into the reaction chamber.

[0024] In an optional embodiment, a second annular groove is formed on the inner side wall of the connecting tube, and the second annular groove is used to install the third annular rib or the fourth annular rib of the reaction tube.

[0025] The beneficial effects of the embodiments of the present invention include:

[0026] The tube cap structure includes a tube cap body and a punctureable seal. The tube cap body is used to be installed in the opening, and the punctureable seal is provided on the tube cap body to seal the reaction chamber. After the tube cap body is installed in the opening, the punctureable seal can seal the reaction chamber. When performing nucleic acid testing, it is only necessary to set the connecting tube of the reagent cartridge and the reaction tube body, and then puncture the punctureable seal with the fluid supply tip of the reagent cartridge, and inject the extracted nucleic acid sample and reaction reagent into the reaction chamber, and then reconstitute with the freeze-dried reagent in the reaction chamber to react; the entire operation process is simple, there is no need to remove the tube cap structure, and the sealing effect during the reaction process is good. Therefore, the tube cap structure can avoid contamination of the reaction system, is convenient to operate, and improves work efficiency.

[0027] The reaction tube comprises a reaction tube body and a tube cap structure. The reaction tube body has a reaction chamber and an opening. The tube cap body is mounted on the opening so that a punctureable seal seals the reaction chamber. The reaction tube body is used to receive a connecting tube of a reagent cartridge so that a fluid supply tip of the reagent cartridge punctures the punctureable seal. The reaction tube has all the benefits of the tube cap structure.

[0028] The reagent cartridge includes a connecting tube, a fluid supply tip, and a reaction tube. A portion of the fluid supply tip is disposed within the connecting tube, while another portion extends out of the connecting tube. The fluid supply tip has a flow channel for circulating nucleic acid samples and reaction reagents. The connecting tube is sleeved onto the reaction tube body, and the fluid supply tip is used to pierce a pierceable seal to allow the nucleic acid sample and reaction reagents to flow into the reaction chamber. This reagent cartridge has all the benefits of the reaction tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0030] Figure 1 A schematic structural diagram of a reagent cartridge provided in an embodiment of the present invention;

[0031] Figure 2 An exploded view of a reagent cartridge provided in an embodiment of the present invention;

[0032] Figure 3 A schematic structural diagram of a reaction tube provided in an embodiment of the present invention;

[0033] Figure 4 An exploded view of a reaction tube provided in an embodiment of the present invention;

[0034] Figure 5 A schematic structural diagram of a pipe cap structure provided by an embodiment of the present invention from a first perspective;

[0035] Figure 6 A schematic structural diagram of a pipe cap structure provided by an embodiment of the present invention from a second perspective;

[0036] Figure 7 A schematic diagram of the assembly process of a reaction tube provided in an embodiment of the present invention for freeze-drying reagents;

[0037] Figure 8 A schematic diagram of the assembly process of the reagent cartridge provided in an embodiment of the present invention when performing a freeze-dried reagent reaction;

[0038] Figure 9 A schematic structural diagram of a connecting tube and a fluid supply tip provided in an embodiment of the present invention.

[0039] Icons: 1000-reagent cartridge; 1100-reaction tube; 100-tube cap structure; 10-tube cap body; 11-pipeline; 12-first port; 13-second port; 14-vent; 15-first annular rib; 16-second annular rib; 20-puncturable seal; 200-reaction tube body; 210-reaction chamber; 220-opening; 230-first tube body; 231-third annular rib; 232-fourth annular rib; 233-first annular groove; 240-second tube body; 1200-connecting tube; 1210-second annular groove; 1300-fluid supply tip; 1310-flow channel; 1400-syringe. DETAILED DESCRIPTION

[0040] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings herein can be arranged and designed in various different configurations.

[0041] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention as claimed, but rather merely represents selected embodiments of the present invention. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort shall fall within the scope of protection of the present invention.

[0042] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0043] In the description of the present invention, it should be noted that if the terms "upper", "lower", "inside", "outside", etc. appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the accompanying drawings, or is the orientation or position relationship in which the product of the invention is usually placed when in use. It is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be understood as a limitation on the present invention.

[0044] In addition, the terms "first", "second", etc., if used, are merely used to distinguish and describe, and should not be understood as indicating or implying relative importance.

[0045] It should be noted that, in the absence of conflict, the features in the embodiments of the present invention may be combined with each other.

[0046] As described in the background technology, fluorescent quantitative PCR is the main technology of clinical molecular detection. At present, the method usually uses liquid reagents, which must be transported and stored in a cold chain. The packaging, logistics, and storage costs are high. It can also cause losses due to reasons such as cold chain transportation failure, pipetting errors, repeated freezing and thawing, and contamination of aerosol amplification products. Therefore, fluorescent quantitative PCR detection reagents are mostly made into dry reagents that are stable at room temperature. When performing fully automatic nucleic acid detection, the extracted nucleic acid sample and the re-dissolving reagent need to be transferred to a reaction tube equipped with a freeze-dried reagent in order to perform nucleic acid amplification detection. Since the freeze-dried reagent needs to be sealed and stored, the user generally needs to open the reaction tube and perform amplification detection after adding the sample and reaction reagent. However, adopting this method will bring the risk of contamination to the reaction system, and it is inconvenient for users to use and has low efficiency.

[0047] Based on this, please refer to Figures 1-9 The embodiments of the present invention provide a cap structure 100, a reaction tube 1100, and a reagent cartridge 1000 that effectively address the aforementioned technical issues. Specifically, they prevent contamination of the reaction system, facilitate operation, and enhance efficiency. The cap structure 100, reaction tube 1100, and reagent cartridge 1000 are described in detail below.

[0048] Please refer to Figure 1 and Figure 2 , Figure 1 This is a schematic structural diagram of the reagent cartridge 1000 provided in this embodiment. Figure 2 The exploded view of the reagent cartridge 1000 provided in this embodiment is shown in FIG. Figure 1 and Figure 2 The reagent cartridge 1000 includes a reaction tube 1100, a connecting tube 1200, and a fluid supply tip 1300. The reaction tube 1100 is used to store lyophilized reagents. The connecting tube 1200 is sleeved within the reaction tube 1100. A portion of the fluid supply tip 1300 is disposed within the connecting tube 1200, while another portion of the fluid supply tip 1300 extends out of the connecting tube 1200. During nucleic acid testing, the nucleic acid sample and reaction reagents flow into the reaction tube 1100 through the fluid supply tip 1300, and after being reconstituted with the lyophilized reagents in the reaction tube 1100, a reaction occurs, and subsequent nucleic acid amplification testing is then performed.

[0049] Specifically, please refer to Figure 3 and Figure 4 , Figure 3 This is a schematic structural diagram of the reaction tube 1100 provided in this embodiment. Figure 4 The exploded view of the reaction tube 1100 provided in this embodiment is combined with Figure 3 and Figure 4The reaction tube 1100 includes a tube cap structure 100 and a reaction tube body 200. The reaction tube body 200 is sleeved on the tube cap structure 100. The reaction tube body 200 has a reaction chamber 210 and an opening 220. The tube cap structure 100 is installed in the opening 220. The reaction chamber 210 is used to accommodate the freeze-dried reagent.

[0050] For further information, please refer to Figure 5 and Figure 6 , Figure 5 This is a structural diagram of the pipe cap structure 100 provided in this embodiment from a first perspective. Figure 6 A schematic structural diagram of the pipe cap structure 100 provided in this embodiment from a second perspective, combined with Figure 5 and Figure 6 The tube cap structure 100 includes a tube cap body 10 and a puncturable seal 20. The tube cap body 10 is used to be installed in the opening 220, wherein the puncturable seal 20 can be made of a plastic sealing film, a metal foil sealing film, etc., which is easy to puncture. The puncturable seal 20 is provided on the tube cap body 10 to seal the reaction chamber 210. After the tube cap body 10 and the opening 220 are installed, the puncturable seal 20 can seal the reaction chamber 210. When performing nucleic acid testing, it is only necessary to put the connecting tube 1200 of the reagent cartridge 1000 and the reaction tube body 200 together, and then puncture the puncturable seal 20 through the fluid supply tip 1300 of the reagent cartridge 1000, and inject the extracted nucleic acid sample and reaction reagent into the reaction chamber 210 to react with the freeze-dried reagent in the reaction chamber 210; the entire operation process is simple, there is no need to remove the tube cap structure 100, and the sealing effect during the reaction process is good. Therefore, the tube cap structure 100 can avoid contamination of the reaction system, is easy to operate, and improves work efficiency.

[0051] Specifically, in this embodiment, the cap body 10 has a channel 11 for the fluid supply tip 1300 of the reagent cartridge 1000 to extend into, and the pierceable seal 20 is provided on the channel 11. Figure 2 and Figure 5 That is to say, the pipe 11 is sealed by the punctureable seal 20, thereby sealing the reaction chamber 210; when a reaction is required, the fluid supply tip 1300 is extended into the pipe 11, and the extracted nucleic acid sample and reaction reagent are injected into the reaction chamber 210 by puncturing the punctureable seal 20; due to the isolation effect of the punctureable seal 20, the process can be carried out in a certain sealed environment, preventing the external environment from contaminating the reaction system and subsequent nucleic acid detection, and the entire process does not require the removal of the tube cap structure 100, which is easy to operate and efficient.

[0052] It should be noted that the puncturable seal 20 can be provided at any position of the tube body, as long as the reaction chamber 210 of the reaction tube body 200 can be sealed.

[0053] In order to facilitate the insertion and extension of the fluid supply tip 1300, the cap body 10 further has a first port 12 and a second port 13. The first port 12, the pipeline 11 and the second port 13 are connected in sequence. The first port 12 is used to match the opening 220, and the second port 13 is used to extend into the reaction tube body 200. A pierceable seal 20 is provided at the second port 13 to seal the reaction chamber 210. Figure 3 and Figure 5 When the cap body 10 is installed in the reaction tube body 200, the first port 12 is matched with the opening 220 to achieve the installation of the cap body 10 and the reaction tube body 200. The second port 13 extends into the reaction tube body 200. The outer wall of the second port 13 abuts against the inner wall of the reaction tube body 200, and the pierceable seal 20 seals the second port 13, so that the reaction chamber 210 is in a sealed state.

[0054] It should be noted that, in this embodiment, the puncturable seal 20 is arranged at the second port 13 and seals the second port 13, which can ensure the preload length of the fluid supply tip 1300, so that when the reaction tube body 200 and the connecting tube 1200 are assembled, the fluid supply tip 1300 is first extended into the pipeline 11, and then when the nucleic acid sample and reaction reagent need to be injected into the reaction chamber 210, the reaction tube body 200 and the connecting tube 1200 are further loaded and assembled, so that the fluid supply tip 1300 pierces the puncturable seal 20, thereby ensuring the sealing before the reaction, further avoiding the external environment from polluting the reaction system and causing aerosol contamination during amplification.

[0055] Of course, in some other embodiments, the pierceable seal 20 may be directly provided at the first port 12 , and the pierceable seal 20 may be directly pierced by the fluid or the supply tip when the reaction tube body 200 and the connecting tube 1200 are assembled.

[0056] In addition, it should be noted that in this embodiment, the first port 12, the conduit 11, and the second port 13 are coaxially arranged. Of course, in other embodiments, if the structure of the reaction tube body 200 is changed, the first port 12, the conduit 11, and the second port 13 may also be coaxially arranged. For example, if the reaction chamber 210 of the reaction tube body 200 is bent, the conduit 11 of the cap body 10 also needs to be bent. In this case, the pierceable seal 20 will be arranged on the side wall of the cap body 10. Of course, in this case, the fluid supply tip 1300 also needs to be bent to pierce the pierceable seal 20. In other words, the location and specific structure of the conduit 11, the fluid supply tip 1300, and the pierceable seal 20 need to be determined based on the actual reaction tube body 200.

[0057] During cold chain transportation and storage, the liquid reagent in the reaction tube 1100 needs to be frozen to form a lyophilized reagent for storage and transportation. In order to facilitate the escape of water vapor during lyophilization, please continue to combine Figure 5 and Figure 6 The cap body 10 is provided with a vent 14 which passes through the inner and outer walls of the cap body 10 and is connected to the pipe 11. It should be noted that freeze drying of the liquid reagent has an evaporation effect, which will generate water vapor, which needs to be discharged through the vent 14.

[0058] In order to better implement the freeze-drying of liquid reagents, please combine Figure 4-Figure 6 The outer wall of the tube cap body 10 is further provided with a first annular rib 15, which is located between the first port 12 and the second port 13 and is arranged around the vent 14; the first annular rib 15 is used to separate the vent 14 together with the inner wall of the reaction tube body 200 when abutting the opening 220, so that a part of the vent 14 is connected to the reaction chamber 210, and the other part is connected to the outside.

[0059] Specifically, please refer to Figure 7 , Figure 7 This is a schematic diagram of the assembly process of the reaction tube 1100 provided in this embodiment when performing reagent freeze-drying. It should be noted that: Figure 7 Position A shown in the figure is the first installation position of the cap structure 100 and the reaction tube body 200, and position B is the second installation position of the cap structure 100 and the reaction tube body 200. That is, when freeze-drying a liquid reagent, the first annular rib 15 abuts against the opening 220, placing the cap body 10 in the first installation position and performing freeze-drying of the liquid reagent. At this time, a certain gap exists between the outer wall of the cap body 10 and the inner wall of the reaction tube body 200, allowing moisture generated by the liquid reagent to pass through this gap and escape from the vent 14. After freeze-drying is completed, the cap body 10 is pressed downward to the second installation position. The second port 13 abuts against the inner wall of the reaction tube body 200, and together with the pierceable seal 20, the reaction chamber 210 of the reaction tube body 200 is sealed, thereby achieving sealed preservation of the freeze-dried reagent in the reaction chamber 210.

[0060] Combine Figure 4 In order to conveniently fix the first annular rib 15, a first annular groove 233 is opened on the inner wall of the reaction tube body 200. When the tube cap body 10 is pressed down, when the first annular rib 15 is just stuck in the first annular groove 233, the tube cap body 10 is in the second installation position.

[0061] Furthermore, in order to better fix the tube cap body 10 and the reaction tube body 200, the outer wall of the tube cap body 10 is further provided with a second annular rib 16, which is located at the first port 12 and is used to abut the opening 220. Figure 7 It is easy to understand that when the tube cap body 10 is in the second installation position, the second annular rib 16 just abuts against the opening 220 of the reaction tube body 200, thereby limiting the further downward movement of the tube cap body 10 and achieving a stable fit between the tube cap structure 100 and the reaction tube body 200.

[0062] Please continue to combine Figure 5 and Figure 6 In order to increase the rate of water vapor escape during freeze-drying of liquid reagents, the number of vents 14 is multiple, and the multiple vents 14 are arranged at intervals along the circumference of the tube cap body 10. In this embodiment, the number of vents 14 is four, and at this time, a first annular rib 15 is provided between two adjacent vents 14; of course, in other embodiments, the number of vents 14 can also be two, three, five, etc.

[0063] Please continue to combine Figure 4 In this embodiment, the reaction tube 1100 includes a first tube body 230 and a second tube body 240 that are interconnected. The first tube body 230 is used to receive the connecting tube 1200, and the second tube body 240 defines a reaction chamber 210. The first tube body 230 is sleeved onto the cap body 10 so that the cap body 10 abuts against the inner sidewall of the second tube body 240, and the pierceable seal 20 seals the reaction chamber 210. Specifically, in this embodiment, a first annular groove 233 is provided on the inner sidewall of the first tube body 230.

[0064] It should be noted that, in this embodiment, the diameter of the second tube body 240 gradually decreases in the direction away from the first tube body 230; Figure 7 That is, the second tube body 240 is specifically a structure similar to a cone, so that when the tube cap body 10 is in the first installation position, there is a gap between the tube cap body 10 and the inner wall of the first tube body 230, and the second tube body 240 is not sealed. In this way, when the liquid reagent in the reaction chamber 210 is freeze-dried, the water vapor generated can escape from the vent 14; when the tube cap body 10 is in the second installation position, the second port 13 can abut against the inner wall of the cone structure of the second tube body 240, thereby jointly sealing the second tube body 240 through the pierceable seal 20.

[0065] Furthermore, in order to facilitate the loading and assembly of the first tube body 230 and the connecting tube 1200, a third annular rib 231 is provided on the outer wall of the first tube body 230. The third annular rib 231 is located at one end of the first tube body 230 away from the second tube body 240. Figure 8 , Figure 8Schematic diagram of the assembly process of the reagent cartridge 1000 provided in this embodiment when performing a freeze-dried reagent reaction, combined with Figure 8 It should be noted that the position C shown in the figure is the first loading position of the first tube body 230 and the connecting tube 1200, and the position D is the second loading position of the first tube body 230 and the connecting tube 1200.

[0066] like Figure 8 As shown, when the first tube body 230 is in the first loading position, the third annular rib 231 abuts against the inner sidewall of the connecting tube 1200, securing the first tube body 230 to the connecting tube 1200. At this time, the fluid supply tip 1300 of the reagent cartridge 1000 extends into the channel 11 of the cap structure 100 and is spaced a certain distance from the pierceable seal 20, which can be understood as the pre-loading position of the reaction tube 1100. When the first tube body 230 is in the second loading position, the fluid supply tip 1300 pierces the pierceable seal 20, and the nucleic acid sample and reaction reagent are injected into the reaction chamber 210 through the flow channel 1310 of the fluid supply tip 1300 and react with the lyophilized reagent. This second loading position can be understood as the loading reaction position.

[0067] It should be noted that the movement of the first tube 230 from the first loading position to the second loading position can be moved into place by an external power mechanism to improve work efficiency. Of course, in some embodiments, loading can also be done manually.

[0068] In addition, combined Figure 1 and Figure 2 It should also be noted that, in this embodiment, in order to facilitate the injection of nucleic acid samples and reaction reagents into the reaction chamber 210, the reagent cartridge 1000 further includes a syringe 1400, which is connected to the flow channel 1310 for injecting nucleic acid samples and reaction reagents into the flow channel 1310.

[0069] In order to better fix the third annular rib 231, please refer to Figure 9 , Figure 9 This is a schematic diagram of the structure of the connecting tube 1200 and the fluid supply tip 1300 provided in this embodiment, combined with Figure 8 and Figure 9 A second annular groove 1210 is provided on the inner wall of the connecting tube 1200, and the second annular groove 1210 is used to install the third annular rib 231 of the reaction tube 1100; that is, when the first tube body 230 is in the first loading position, the third annular rib 231 is just stuck in the second annular groove 1210, further improving the stability of the connecting tube 1200 and the first tube body 230.

[0070] Since the third annular rib 231 will leave the second annular groove 1210 when the first tube body 230 moves from the first loading position to the second loading position, in order to ensure that the first tube body 230 can also maintain stability when it is in the second loading position, the third annular rib 231 will leave the second annular groove 1210. Figure 4 In this embodiment, the outer wall of the first tube body 230 is further provided with a fourth annular rib 232. The fourth annular rib 232 and the third annular rib 231 are spaced apart along the axial direction of the first tube body 230. The second annular groove 1210 is also used to receive the fourth annular rib 232 of the reaction tube 1100. That is, when the first tube body 230 is in the second loading position, the fourth annular rib 232 fits neatly into the second annular groove 1210, ensuring stability during the reaction system.

[0071] Based on the above description, the working process of nucleic acid detection using the reagent cartridge 1000 is described in detail below:

[0072] First, add the liquid reagent into the reaction chamber 210 of the reaction tube body 200, then put the reaction tube body 200 on the tube cap structure 100, and make the tube cap body 10 be in the first installation position, that is, the first annular rib 15 abuts against the opening 220; then, freeze-dry the liquid reagent. During the freeze-drying process, the water vapor generated by the liquid reagent escapes through the vent 14; after the freeze-drying is completed, press the tube cap body 10 to make it in the second installation position, that is, the first annular rib 15 is clamped in the first annular groove 233, and the second annular rib 16 abuts against the opening 220, so that the second port 13 and the pierceable seal 20 seal the reaction chamber 210 of the second tube body 240, completing the sealing of the freeze-dried reagent; then, put the assembled reaction tube 1100 and the connecting tube 1200 together, First, the first tube body 230 is set in the first loading position, that is, the third annular rib 231 is engaged with the second annular groove 1210. At this time, the fluid supply tip 1300 extends into the pipe 11 of the tube cap body 10 and has a distance from the pierceable seal 20, thereby realizing the preloading of the reaction tube 1100; then, the external power mechanism is operated to drive the reaction tube 1100, so that the first tube body 230 is in the second loading position, that is, the third annular rib 231 moves up and disengages from the second annular groove 1210, and the fourth annular rib 232 is engaged with the second annular groove 1210. At this time, the fluid supply tip 1300 pierces the pierceable seal 20, and the extracted nucleic acid sample and reaction reagent are injected into the reaction chamber 210 through the flow channel 1310, and react with the freeze-dried reagent, and finally the nucleic acid amplification detection is performed.

[0073] In summary, embodiments of the present invention provide a cap structure 100, a reaction tube 1100, and a reagent cartridge 1000. The cap structure 100 includes a cap body 10 and a punctureable seal 20. The cap body 10 is mounted on the opening 220. The punctureable seal 20 is disposed on the cap body 10 to seal the reaction chamber 210. After the cap body 10 and the opening 220 are mounted, the punctureable seal 20 can seal the reaction chamber 210. When performing nucleic acid testing, the connecting tube 1200 of the reagent cartridge 1000 is simply assembled with the reaction tube body 200. The punctureable seal 20 is then punctured by the fluid supply tip 1300 of the reagent cartridge 1000, and the extracted nucleic acid sample and reaction reagents are injected into the reaction chamber 210 to react with the lyophilized reagent in the reaction chamber 210. The entire operation is simple, there is no need to remove the cap structure 100, and the sealing effect is good during the reaction process. Therefore, the tube cap structure 100 can avoid contamination of the reaction system, is easy to operate, and improves work efficiency.

[0074] The reaction tube 1100 includes a reaction tube body 200 and a cap structure 100. The reaction tube body 200 has a reaction chamber 210 and an opening 220. The cap body 10 is mounted in the opening 220 so that the pierceable seal 20 seals the reaction chamber 210. The reaction tube body 200 is used to receive the connecting tube 1200 of the reagent cartridge 1000 so that the fluid supply tip 1300 of the reagent cartridge 1000 pierces the pierceable seal 20. The reaction tube 1100 has all the functions and benefits of the cap structure 100.

[0075] The reagent cartridge 1000 includes a connecting tube 1200, a fluid supply tip 1300, and a reaction tube 1100. A portion of the fluid supply tip 1300 is disposed within the connecting tube 1200, while another portion extends out of the connecting tube 1200. The fluid supply tip 1300 has a flow channel 1310 for circulating nucleic acid samples and reaction reagents. The connecting tube 1200 is sleeved within the reaction tube body 200, and the fluid supply tip 1300 is used to pierce the pierceable seal 20, allowing the nucleic acid sample and reaction reagents to flow into the reaction chamber 210. This reagent cartridge 1000 has all the functions and benefits of the reaction tube 1100.

[0076] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present invention should be included in the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the scope of protection of the claims.

Claims

1. A tube cap structure, adapted to fit a reaction tube body (200), wherein the reaction tube body (200) has a reaction chamber (210) and an opening (220), characterized in that: The pipe cap structure (100) comprises: a pipe cap body (10), the pipe cap body (10) being used to be mounted on the opening (220); and A puncturable seal (20) is provided on the tube cap body (10) for sealing the reaction chamber (210).

2. The pipe cap structure according to claim 1, characterized in that: The tube cap body (10) has a channel (11) for the fluid supply tip (1300) of the reagent cartridge (1000) to extend into, and the pierceable seal (20) is arranged on the channel (11).

3. The pipe cap structure according to claim 2, characterized in that: The pipe cap body (10) is provided with a vent (14), which passes through the inner wall and the outer wall of the pipe cap body (10) and is communicated with the pipe (11).

4. The pipe cap structure according to claim 3, characterized in that: The pipe cap body (10) further comprises a first port (12) and a second port (13); an outer side wall of the pipe cap body (10) is provided with a first annular rib (15); the first annular rib (15) is located between the first port (12) and the second port (13), and is arranged around the vent (14); The first annular rib (15) is used to separate the vent (14) together with the inner wall of the reaction tube body (200) when abutting against the opening (220), so that a part of the vent (14) is connected to the reaction chamber (210) and the other part is connected to the outside.

5. The pipe cap structure according to claim 4, characterized in that: The first port (12), the pipe (11) and the second port (13) are connected in sequence, the first port (12) is used to match the opening (220), the second port (13) is used to extend into the reaction tube body (200), and the puncturable seal (20) is provided at the second port (13) for sealing the reaction chamber (210).

6. The pipe cap structure according to claim 4, characterized in that: The outer side wall of the tube cap body (10) is further provided with a second annular rib (16), and the second annular rib (16) is located at the first port (12) for abutting against the opening (220).

7. The pipe cap structure according to claim 4, characterized in that: The first port (12), the pipe (11) and the second port (13) are connected in sequence, the first port (12) is used to match the opening (220), the second port (13) is used to extend into the reaction tube body (200), and the puncturable seal (20) is provided at the first port (12) for sealing the reaction chamber (210).

8. A reaction tube, characterized in that: The invention comprises a reaction tube body (200) and a tube cap structure (100) according to any one of claims 1 to 6, wherein the reaction tube body (200) has a reaction chamber (210) and an opening (220), and the tube cap body (10) is installed in the opening (220) so that the puncturable seal (20) seals the reaction chamber (210); the reaction tube body (200) is used to be loaded with a connecting tube (1200) of a reagent cartridge (1000) so that a fluid supply tip (1300) of the reagent cartridge (1000) punctures the puncturable seal (20).

9. The reaction tube according to claim 8, characterized in that The reaction tube body (200) comprises a first tube body (230) and a second tube body (240) which are connected to each other. The first tube body (230) is used to accommodate the connecting tube (1200). The second tube body (240) has the reaction chamber (210). The first tube body (230) is sleeved on the tube cap body (10) so that the tube cap body (10) abuts against the inner wall of the second tube body (240) and the puncturable seal (20) seals the reaction chamber (210).

10. The reaction tube according to claim 9, characterized in that The outer wall of the first tube body (230) is provided with a third annular rib (231), and the third annular rib (231) is located at one end of the first tube body (230) away from the second tube body (240). The outer wall of the first tube body (230) is also provided with a fourth annular rib (232), and the fourth annular rib (232) and the third annular rib (231) are spaced apart along the axial direction of the first tube body (230).

11. The reaction tube according to claim 9, characterized in that The diameter of the second tube body (240) gradually decreases in a direction away from the first tube body (230).

12. The reaction tube according to claim 9, characterized in that A first annular groove (233) is provided on the inner side wall of the first tube body (230), and the first annular groove (233) is used for installing the first annular rib (15) of the tube cap structure (100).

13. A reagent cartridge, characterized in that: The invention comprises a connecting tube (1200), a fluid supply tip (1300), and the reaction tube (1100) according to any one of claims 8 to 12, wherein a portion of the fluid supply tip (1300) is disposed in the connecting tube (1200), another portion of the fluid supply tip (1300) extends out of the connecting tube (1200), and the fluid supply tip (1300) has a flow channel (1310) for circulating nucleic acid samples and reaction reagents; The connecting tube (1200) is sleeved on the reaction tube body (200), and the fluid supply tip (1300) is used to pierce the pierceable seal (20) to allow the nucleic acid sample and the reaction reagent to flow into the reaction chamber (210).

14. The reagent cartridge according to claim 13, wherein: A second annular groove (1210) is provided on the inner side wall of the connecting tube (1200), and the second annular groove (1210) is used for mounting the third annular rib (231) or the fourth annular rib (232) of the reaction tube (1100).

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