Method and device for measuring three-dimensional distribution of medicinal and edible composition

Abstract

The invention discloses a method and a device for measuring three-dimensional distribution of a medicinal and edible composition, and belongs to the technical field of plant detection. The composition slices are gasified through laser ablation to generate gas molecules, the molecular structure of the target component is reserved, the gasification completion moment is judged according to the first spectral data, impurity gas in the gas molecules is reduced, then the gas molecules are ionized through the ionization chamber to generate sample ions, the ionization efficiency is improved, and the target component is obtained. The ion signal intensity is increased, interference is little, target ions and other ions in sample ions are separated according to the mobility chamber and the flight channel, and the detection precision is improved. Furthermore, according to the two-dimensional data and the thickness of the composition slice, a plurality of three-dimensional slice models are generated according to the content of the target component, a three-dimensional content model of the to-be-detected object is established, and the content and the uniform distribution degree of the target component in the to-be-detected object are clearly displayed.

Description

Technical Field

[0001] The present invention relates to the technical field of plant detection, and in particular to a method and device for measuring the three-dimensional distribution of a medicinal and edible composition. Background Art

[0002] Hawthorn, as a Chinese medicinal material and food that is both a medicine and a food, has multiple effects and functions and can be used to prepare a medicine-food composition. Quercetin is one of the key active ingredients in hawthorn, and has antioxidant, anti-inflammatory, cardiovascular protection and other effects. In hawthorn, substances with a mass-to-charge ratio similar to that of quercetin, such as kaempferol and isorhamnetin, need to be distinguished from substances with similar mass-to-charge ratios in order to obtain the content of quercetin. The Chinese patent application with publication number CN120314418A discloses a method for determining mangiferin in a mango extract, wherein the method first ionizes sample ions from a target material, and at least part of the sample ions enters a mobility cell, and mass spectrometry data is generated after capturing the sample ions, and the first sampling data and the second sampling data are extracted from the mass spectrometry data. The invention directly ionizes sample ions from the target material, has too many impurities, and has a large degree of fragmentation ions, which is not conducive to the later separation of target detection objects, substances with similar mass-to-charge ratios, and isomers according to the mobility cell, thereby reducing the detection accuracy. Medicinal and edible compositions are typically available in solid forms such as extracts and pills. Due to the uneven mixing of raw materials, multiple slices are required to obtain a three-dimensional distribution of the active ingredients in these compositions in order to more accurately determine the content of each active ingredient. Therefore, further improvements are needed in the existing technology. Summary of the Invention

[0003] In order to solve the defects of the above-mentioned prior art, the present invention proposes a method and device for measuring the three-dimensional distribution of a medicinal and edible composition. The present invention first slices the object to be detected to obtain multiple slices to be detected, vaporizes the slices to be detected by laser ablation to generate gas molecules, judges the completion time of vaporization according to the first spectral data, reduces the impurity gas in the gas molecules, and then ionizes the gas molecules through the ionization chamber to generate sample ions, separates the target ions and impurity ions in the sample ions according to the mobility chamber and the flight channel, obtains the content of the target component, and improves the detection accuracy. Further, based on the two-dimensional data, thickness and content of the target component of the slice to be detected, multiple three-dimensional slice models are generated, and then a three-dimensional content model of the object to be detected is established, which clearly displays the content and uniform distribution of the target component in the object to be detected, expands the scope of application, and facilitates data analysis.

[0004] The technical solution of the present invention is achieved as follows: A method for measuring the three-dimensional distribution of a medicinal and edible composition comprises the following steps: Step 1: Place the sample to be detected on the experimental table, and vertically align the first light emitter with the sample to be detected on the experimental table to emit a first laser; Step 2: The sample to be detected generates gas molecules, and the optical transceiver emits a second laser to the gas molecules to generate first spectrum data. The first light emitter and the optical transceiver are turned off according to the first spectrum data, and the first moment is recorded. Step 3: Open the first and second valves to introduce carrier gas, allowing gas molecules to enter the ionization chamber. The ionization chamber generates an electrospray to ionize the gas molecules to produce sample ions. Open the third valve to introduce the sample ions into the mobility chamber, and record the second moment. Step 4: The sample ions pass through the mobility chamber and enter the flight channel. The mass analyzer captures the sample ions and generates the first ion mobility spectrum, intercepting the sampling data. Step 5: Repeat steps 1 to 4 to obtain a sample data set, optimize the erosion parameters, ionization parameters, and mobility parameters, obtain a standard curve for the target component, prepare multiple composite slices of the object to be detected, and place the composite slices on the laboratory bench in the order of slicing; Step 6: Obtain the two-dimensional data and thickness of the composite slice and divide the target grid; Step 7: Move the first optical transmitter and optical transceiver according to the target grid, repeat steps 1 to 4, obtain a second ion mobility spectrum, and calculate the content of the target component in the target grid; Step 8: If all target grids are traversed, determine whether all composite slices are traversed. If so, proceed to step 9. Otherwise, place the next composite slice on the test bench and return to step 6. Otherwise, proceed to the next target grid and return to step 7. Step 9: Generate multiple three-dimensional slice models based on the two-dimensional data and thickness of the composite slices and the content of the target components in multiple target grids to establish a three-dimensional content model of the object to be detected.

[0005] In the present invention, in step 1, the experimental table includes a glass cover and a sample table, the glass cover maintains a vacuum environment, the sample to be detected is a thin slice, the sample to be detected is placed on the sample table, and the laser energy is initially set to the reference energy.

[0006] In the present invention, in step 2, the optical transceiver includes a second optical transmitter and a signal acquisition device, the signal acquisition device is installed with a bandpass filter, the signal acquisition device receives the fluorescence signal to generate first spectral data, the first spectral data is fluorescence spectral data, when the fluorescence signal drops to the baseline, the first optical transmitter and the optical transceiver are turned off, and the first moment is the moment when the first optical transmitter and the optical transceiver are turned off.

[0007] In the present invention, in step 3, the first valve controls the carrier gas to enter the experimental table, the second valve controls the gas molecules to enter the ionization chamber, and the third valve controls the sample ions to enter the mobility chamber. The carrier gas brings the gas molecules into the ionization chamber, and the sample ions are introduced into the mobility chamber through the ion funnel. The carrier gas is helium, the ionization chamber is in negative ion mode, the electrospray contains methanol, water and formic acid, the mass fraction of formic acid is 0.1%, and the volume ratio of methanol and water is 70%:30%. The second moment is the moment when the third valve is opened.

[0008] In the present invention, in step 4, the first migration interval is calculated based on the first properties of the target component and the buffer gas, the first flight interval is calculated based on the second properties of the target component and the buffer gas, the sampling interval of the target component is calculated according to the first migration interval and the first flight interval, and then the minimum sampling moment and the maximum sampling moment of the target component are obtained in combination with the second moment and the sampling interval, and sampling data is intercepted from the first ion mobility spectrum according to the minimum sampling moment and the maximum sampling moment.

[0009] In the present invention, in step 4, the mobility chamber contains a buffer gas, the buffer gas is helium, the flight channel is a high vacuum environment, the sampling data is the target ion peak, the target component is quercetin, the first attribute is the ion collision cross section, and the second attribute is the mass-to-charge ratio.

[0010] In the present invention, in step 5, the sampling data set includes sampling data under multiple different parameters, and an algorithm parameter set is obtained according to the sampling data set, wherein the algorithm parameter set includes a symmetry factor and a peak width. An objective function is established according to the algorithm parameter set, and the erosion parameters, ionization parameters, and mobility parameters are optimized according to the objective function and the optimization algorithm to obtain target erosion parameters, target ionization parameters, and target mobility parameters. The object to be detected is a medicinal and edible composition.

[0011] In the present invention, in step 7, the center of the light spot of the first light emitter is aligned with the center of the target grid, the first laser is perpendicular to the laboratory table, the optical transceiver is parallel to the laboratory table, the second laser is not in the same plane as the first laser, the minimum sampling time and the maximum sampling time are recalculated, and the target ion peak of the second ion mobility spectrum is intercepted, and the content of the target component in the target grid is calculated based on the standard curve and the second ion mobility spectrum.

[0012] In the present invention, in step 9, the target grid is aligned with the two-dimensional data of the composition slice, the two-dimensional data is filled with the content of the target component in the corresponding target grid, and a three-dimensional slice model is generated according to the thickness widening of the two-dimensional data. Multiple three-dimensional slice models are stacked in sequence to establish a three-dimensional content model of the object to be detected.

[0013] A measuring device for realizing the method for measuring the three-dimensional distribution of the edible and medicinal composition, comprising: A laboratory table comprising a glass cover and a sample table, wherein the glass cover maintains a vacuum environment and the sample table holds samples of objects to be tested and slices of the composition; a first light emitter, configured to emit a first laser; An optical transceiver, comprising a second light emitter and a signal acquisition device, wherein the second light emitter is used to emit a second laser, and the signal acquisition device is used to collect a fluorescence signal to generate first spectral data; an ionization chamber, which is used to generate electrospray to ionize gas molecules to produce sample ions; The mobility cell is used to distinguish the target ion from other ions with similar mass-to-charge ratios; The flight channel is used to separate target ions and other ions with different mass-to-charge ratios in sample ions through free flight in a high vacuum environment; The mass analyzer is used to capture sample ions and generate a first ion mobility spectrum and a second ion mobility spectrum; a data processing device configured to calculate a minimum sampling time and a maximum sampling time, intercept a target ion peak from the second ion mobility spectrum, obtain the content of the target component in the target grid according to a standard curve, and establish a three-dimensional content model of the object to be detected; a data analysis device configured to analyze the first moment based on the first spectral data, obtain an algorithm parameter set based on the sampled data set, establish an objective function based on the algorithm parameter set, and optimize the ablation parameter, the ionization parameter, and the mobility parameter based on the objective function and the optimization algorithm; A three-dimensional positioning system is used to obtain two-dimensional data and thickness of a composite slice, divide the target grid, move the first light emitter and the light transceiver according to the target grid, and replace the composite slice to complete the measurement.

[0014] The method and device for measuring the three-dimensional distribution of a medicinal and edible composition according to the present invention have the following beneficial effects: laser ablation is used to vaporize the composition slices to generate gas molecules, preserving the molecular structure of the target component. The completion time of vaporization is determined based on the first spectral data. The gas molecules are then ionized by an ionization chamber to generate sample ions, reducing impurities in the gas molecules, improving ionization efficiency, increasing ion signal intensity with minimal interference, and separating the target ions from other ions in the sample ions using the mobility chamber and flight channel, thereby improving detection accuracy. Furthermore, based on the two-dimensional data, thickness, and content of the target component of the composition slices, multiple three-dimensional slice models are generated to establish a three-dimensional content model of the object to be detected, clearly displaying the content and uniformity of the target component in the object to be detected. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Flow chart of the method for measuring the three-dimensional distribution of the edible and medicinal composition of the present invention; Figure 2 Schematic diagram of the experimental platform of the present invention; Figure 3 Schematic diagram of the target ion peak in the ion mobility spectrum of the present invention; Figure 4 A schematic diagram of a target grid division diagram of the present invention; Figure 5 Schematic diagram of the first laser and the second laser of the present invention; Figure 6 A schematic diagram of establishing a three-dimensional content model for the present invention; Figure 7 Schematic diagram of the method for measuring the three-dimensional distribution of the edible and medicinal composition of the present invention; Figure 8 The present invention is a block diagram of an apparatus for measuring the three-dimensional distribution of a medicinal and edible composition.

[0016] Figure marks in the accompanying drawings: ionization channel 100, first pipeline 101, second pipeline 102, first collimation channel 200, mobility cell 300, entrance gate 301, exit gate 302, third pipeline 303, second collimation channel 400, flight channel 500, repulsion zone 501, acceleration zone 502, field-free zone 503, first reflection zone 504, second reflection zone 505, exhaust pipeline 506, mass analyzer 600. DETAILED DESCRIPTION

[0017] In order to more clearly understand the purpose, technical solutions and advantages of the present application, the present application is described and illustrated below in conjunction with the accompanying drawings and embodiments.

[0018] Because direct ionization results in a high number of fragmented ions and impurity ions of the target component in the analyte, the subsequent mass analyzer detects numerous ion peaks and weak ion signal intensity, reducing detection accuracy and prolonging detection time. Laser ablation of the analyte slices produces gas molecules that preserve the molecular structure of the target component (avoiding thermal decomposition or fragmentation). The intact molecular structure of the target component (quercetin) combined with the electrospray droplets results in higher ionization efficiency and a greater tendency to form quasi-molecular ions (such as the target ion with a mass-to-charge ratio of 301.03). This results in high ion signal intensity and minimal interference, resulting in a cleaner ion mobility spectrum, avoiding overlapping peaks, and facilitating detection.

[0019] Among the analytes, substances with similar mass-to-charge ratios to quercetin primarily include flavonoid isomers (such as kaempferol and isorhamnetin) and flavonoid glycoside dissociation products. Because the target ion of quercetin (with a mass-to-charge ratio of 301.03) differs in structure and collision area from the characteristic ions of flavonoid isomers, the mobility cell applies an electric field to cause different migration velocities of different sample ions upon collision with the buffer gas, thereby separating the target ion. This ensures that the target ion and the characteristic ions of the flavonoid isomers arrive at the mass analyzer at different times, eliminating overlap between the target ion peak and the peaks corresponding to other ions in the first ion mobility spectrum, thereby improving detection accuracy. The flight channel operates in a high vacuum environment. Due to the different mass-to-charge ratios of the target ion and other ions, the target ion and other ions in the sample are separated by free flight in this high vacuum environment. Example 1

[0020] like Figures 1 to 6 As shown, the method for measuring the three-dimensional distribution of the edible and medicinal composition of the present invention includes the following steps.

[0021] Step 1: Place the sample to be detected on the experimental table, and the first light emitter is vertically aligned with the sample to be detected on the experimental table to emit the first laser. Figure 2 As shown, the experimental bench includes a glass cover and a sample table. A vacuum environment is maintained inside the glass cover, and the sample to be detected is placed on the sample table. The sample to be detected is a thin slice, and the first light emitter can be an ultraviolet nanosecond laser. The wavelength of the first laser is 266nm, and the laser energy is initially set to a reference energy, which can be set to 0.1mJ / pulse. The reference energy cannot be too large. If the reference energy is too large, it will easily lead to rapid heating, thermal decomposition of the target component, and premature disappearance of the fluorescence signal. At the same time, all impurities such as sugars, pigments, and pectin in the sample to be detected are thermally decomposed to produce volatile organic compounds. In this embodiment, the target component is quercetin.

[0022] Step 2: The sample to be detected generates gas molecules. The optical transceiver emits a second laser beam toward the gas molecules, generating first spectral data. Based on the first spectral data, the first optical emitter and optical transceiver are shut down, and the first moment is recorded. The optical transceiver includes a second optical emitter and a signal acquisition device. The wavelength of the second laser beam is 370 nm, and the signal acquisition device is equipped with a bandpass filter ranging from 500 nm to 550 nm. The target component (quercetin) generates a fluorescence signal (fluorescence peak at 520 nm) induced by the second laser beam. The bandpass filter blocks fluorescence from pyrolysis products (such as pectin and other impurities) and laser scattering. The signal acquisition device receives the fluorescence signal and generates the first spectral data. The first spectral data is the fluorescence spectrum data. When the fluorescence signal drops to baseline, the first optical emitter and optical transceiver are shut down. The first moment is the moment the first optical emitter and optical transceiver are shut down. Since the target component has a vaporization temperature of approximately 200°C-280°C and a decomposition temperature of 250°C-350°C, the target component is preferentially vaporized by adjusting the laser energy of the first light emitter to control the temperature at 200°C-250°C, while impurities such as sugars remain due to carbonization. The time required for complete vaporization of the target component in the composite slice is analyzed based on the first spectral data, thereby reducing the amount of impurity gas entering the ionization chamber.

[0023] Step 3: Open the first and second valves, introduce carrier gas, and the gas molecules enter the ionization chamber. The ionization chamber generates electrospray to ionize the gas molecules to generate sample ions. Open the third valve to introduce the sample ions into the mobility chamber and record the second moment. Figure 2 As shown, the glass cover contains a first valve and a second valve. The first valve controls the entry of carrier gas into the laboratory bench, while the second valve controls the entry of gas molecules into the ionization chamber. The carrier gas carries gas molecules into the ionization chamber, where sample ions are introduced into the mobility chamber via an ion funnel. The carrier gas is helium, and the ionization chamber is in negative ion mode. The electrospray contains methanol, water, and formic acid, with a mass fraction of 0.1% formic acid and a volume ratio of 70%:30% methanol:water. The third valve controls the entry of sample ions into the mobility chamber, and the second moment is when the third valve opens.

[0024] Step 4: Sample ions pass through the mobility chamber and enter the flight channel. The mass analyzer captures the sample ions, generates a first ion mobility spectrum, and extracts sampling data. The mobility chamber contains a buffer gas, helium. Sample ions are concentrated by an ion funnel and filtered through the carrier gas before being introduced into the mobility chamber to avoid affecting the mobility pressure of the mobility chamber. A first migration interval is calculated based on the first property of the target component and the buffer gas. A first flight interval is calculated based on the second property of the target component and the buffer gas. The sampling interval of the target component is calculated based on the first migration interval and the first flight interval. The minimum and maximum sampling times of the target component are then calculated based on the second time and the sampling interval. Sampling data is extracted from the first ion mobility spectrum based on these minimum and maximum sampling times. The flight channel is maintained in a high vacuum environment, and the sampling data is the target ion peak. The first property is the ion collision cross section, and the second property is the mass-to-charge ratio.

[0025] Step 5: Repeat steps 1 to 4 to obtain a sampling data set, optimize the erosion parameters, ionization parameters, and mobility parameters, obtain a standard curve for the target component, prepare multiple composition slices of the object to be detected, and place the composition slices on the laboratory table in the order of slicing. The erosion parameters include the energy and spot size of the first laser. The ionization parameters include the ionization voltage. The mobility parameters include the electric field gradient and the electric field intensity. The flight parameters include the acceleration voltage, the flight path length, and the pulse frequency. The sampling data set includes sampling data under multiple different parameters, and an algorithm parameter set is obtained based on the sampling data set, and the algorithm parameter set includes the symmetry factor and the peak width. An objective function is established based on the algorithm parameter set, and the erosion parameters, ionization parameters, and mobility parameters are optimized based on the objective function and the optimization algorithm to obtain target erosion parameters, target ionization parameters, and target mobility parameters. The optimization algorithm can be a particle swarm algorithm, an ant colony algorithm, etc.

[0026] like Figure 3 As shown, the abscissa is time and the ordinate is ion signal intensity. The symmetry factor is the peak symmetry of the target ion peak in the ion mobility spectrum, which reflects the transmission efficiency of the sample ions. Its value is equal to the ratio of the peak front width of the target ion peak (the horizontal distance from 5% of the peak front to 50% of the peak height) to the peak trailing edge width (the horizontal distance from 50% of the peak height to 95% of the peak trailing edge). The peak width is the width of the target ion peak in the ion mobility spectrum, usually expressed as the full width at half maximum, that is, the width of the target ion peak at half its height. The narrower the peak width, the better the focus of the target ion (quercetin produces ions with a mass-to-charge ratio of 301.03 in negative ion mode), the less collision loss, and the higher the resolution.

[0027] Step 6: Obtain the 2D data and thickness of the composite slice and divide the target grid. Figure 4As shown, two-dimensional data of the composite slice is obtained by scanning with a three-dimensional positioning system, a slice center is generated according to the two-dimensional data of the composite slice, a grid size is adjusted according to the spot size of the first laser, a target grid is divided by the slice center, and the target grid is displayed by a low-power LED grid, thereby reducing the impact on gasification and detection.

[0028] Step 7: Move the first optical transmitter and optical transceiver according to the target grid, repeat steps 1 to 4, obtain the second ion mobility spectrum, and calculate the content of the target component in the target grid. Figure 5 As shown, the first light emitter and the optical transceiver are both located outside the glass cover. The center of the first light emitter's spot is aligned with the center of the target grid. The first laser is perpendicular to the laboratory table, and the optical transceiver is parallel to the laboratory table. The second laser is not in the same plane as the first laser to prevent the second laser from affecting the vaporization process of the target component. The minimum and maximum sampling times are recalculated, and the target ion peak of the second ion mobility spectrum is intercepted. The standard curve is a curve showing the relationship between the target component content and the intensity of the target ion peak under the target ablation parameters, target ionization parameters, and target mobility parameters.

[0029] Step 8: If all target grids are traversed, determine whether all composite slices are traversed. If so, proceed to step 9. Otherwise, place the next composite slice on the lab bench and return to step 6. Otherwise, proceed to the next target grid and return to step 7.

[0030] Step 9: Generate multiple three-dimensional slice models based on the two-dimensional data and thickness of the composite slice and the content of the target component in multiple target grids, and establish a three-dimensional content model of the object to be detected. Figure 6 As shown, according to the filled arrow, the target grid is aligned with the two-dimensional data of the composite slice, the content of the target component in the corresponding target grid is filled with the two-dimensional data (x, y, S), and the two-dimensional data is widened according to the thickness to generate a three-dimensional slice model (x, y, D i , S), align the slice center of each composite slice with the three-dimensional slice model, stack multiple three-dimensional slice models in sequence to establish a three-dimensional content model (x, y, D, S) of the object to be detected, where x is the horizontal coordinate of the target grid, y is the vertical coordinate of the target grid, S is the content of the target component, and D i is the thickness of the composition slice i, and D is the thickness of the object to be detected. The content and uniform distribution of the target component are clearly displayed based on the three-dimensional content model of the object to be detected. Example 2

[0031] like Figure 7 As shown, this embodiment further discloses the principle of the method for measuring the three-dimensional distribution of the medicine-food composition.

[0032] Carrier gas flows from the second valve port at a predetermined flow rate (1 L / min) and enters the ionization chamber 100 through the first conduit 101. The first conduit 101 is heated to a predetermined temperature to prevent condensation of gas molecules. Electrospray enters the ionization chamber 100 through the second conduit 102. The electrospray generated in the ionization chamber 100 ionizes the gas molecules to produce sample ions. The ionized sample ions are collected by an ion funnel and filtered by the carrier gas before entering the first collimator channel 200. Buffer gas enters the mobility cell 300 through the third conduit 103. The mobility cell 300 has an entrance gate 301 and an exit gate 302. When these gates are open, sample ions can freely enter and exit. The first collimator channel 200 modulates the sample ions into a first ion beam. When the exit gate 302 is open, at least a portion of the sample ions enter the second collimator channel 400, which modulates the sample ions into a second ion beam, which is then directed into the flight channel 500. In order to reduce other interferences, an exhaust duct 506 may be provided on the side wall of the flight channel 500 to reduce the internal air pressure.

[0033] When the second ion beam enters the flight channel 500, its vertical speed is first accelerated by the repulsion zone 501 and the acceleration zone 502. When the second ion beam enters the field-free zone 503, it maintains a uniform speed. Then, under the action of the first reflection zone 504 and the second reflection zone 505, it is first decelerated to 0 and then accelerated in the opposite direction. When it enters the field-free zone 503 again, it maintains a uniform speed until the second ion beam falls on the mass analyzer 600. However, its horizontal speed remains unchanged during the flight. Example 3

[0034] like Figure 8As shown, a measuring device for implementing the method for measuring the three-dimensional distribution of the medicinal and edible composition comprises: a laboratory bench, a first light emitter, an optical transceiver, an ionization chamber, a mobility chamber, an ion funnel, a flight path, a mass analyzer, a three-dimensional positioning system, a data processing device, and a data analysis device. The laboratory bench includes a glass cover that maintains a vacuum environment and a sample stage for holding a sample to be detected and a slice of the composition. The first light emitter is used to emit a first laser beam to ablate the slice of the composition and the sample to be detected, vaporizing the slice of the composition and the sample to be detected and generating gas molecules. The optical transceiver includes a second light emitter that emits a second laser beam to the gas molecules and a signal acquisition device that collects fluorescence signals to generate first spectral data. The ionization chamber is used to generate an electrospray to ionize the gas molecules to generate sample ions. The mobility chamber is used to distinguish target ions from other ions with similar mass-to-charge ratios. The ion funnel is connected to the ionization chamber and the mobility chamber to filter impurity gases and direct sample ions from the ionization chamber into the mobility chamber. The flight channel separates target ions from other ions with different mass-to-charge ratios within the sample ion system through free flight in a high vacuum environment. The mass analyzer captures the sample ions and generates a primary ion mobility spectrum and a secondary ion mobility spectrum. The 3D positioning system acquires 2D data and thickness of the composite slice, creates a target grid, and moves the primary optical emitter and optical transceiver according to the target grid, replacing the composite slice to complete the measurement.

[0035] The data processing device is configured to calculate the minimum sampling time and the maximum sampling time, extract sampling data from the second ion mobility spectrum based on the minimum sampling time and the maximum sampling time, obtain the content of the target component in the target grid based on the standard curve, and establish a three-dimensional content model of the object to be detected. The data analysis device is configured to analyze the first time based on the first spectral data, obtain an algorithm parameter set based on the sampling data set, establish an objective function based on the algorithm parameter set, and optimize the erosion parameters, ionization parameters, and mobility parameters based on the objective function and the optimization algorithm to obtain target erosion parameters, target ionization parameters, and target mobility parameters.

[0036] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for measuring the three-dimensional distribution of a medicinal and edible composition, characterized in that: The following steps are involved: Step 1: Place the sample to be detected on the experimental table, and vertically align the first light emitter with the sample to be detected on the experimental table to emit a first laser; Step 2: The sample to be detected generates gas molecules, and the optical transceiver emits a second laser to the gas molecules to generate first spectrum data. The first light emitter and the optical transceiver are turned off according to the first spectrum data, and the first moment is recorded. Step 3: Open the first and second valves to introduce carrier gas, allowing gas molecules to enter the ionization chamber. The ionization chamber generates an electrospray to ionize the gas molecules to produce sample ions. Open the third valve to introduce the sample ions into the mobility chamber, and record the second moment. Step 4: The sample ions pass through the mobility chamber and enter the flight channel. The mass analyzer captures the sample ions and generates the first ion mobility spectrum, intercepting the sampling data. Step 5: Repeat steps 1 to 4 to obtain a sample data set, optimize the erosion parameters, ionization parameters, and mobility parameters, obtain a standard curve for the target component, prepare multiple composite slices of the object to be detected, and place the composite slices on the laboratory bench in the order of slicing; Step 6: Obtain the two-dimensional data and thickness of the composite slice and divide the target grid; Step 7: Move the first optical transmitter and optical transceiver according to the target grid, repeat steps 1 to 4, obtain a second ion mobility spectrum, and calculate the content of the target component in the target grid; Step 8: If all target grids are traversed, determine whether all composite slices are traversed. If so, proceed to step 9. Otherwise, place the next composite slice on the test bench and return to step 6. Otherwise, proceed to the next target grid and return to step 7. Step 9: Generate multiple three-dimensional slice models based on the two-dimensional data and thickness of the composite slices and the content of the target components in multiple target grids to establish a three-dimensional content model of the object to be detected.

2. The method for measuring the three-dimensional distribution of the edible and medicinal composition according to claim 1, characterized in that: In step 1, the experimental table includes a glass cover and a sample table. The glass cover maintains a vacuum environment. The sample to be detected is a thin slice. The sample to be detected is placed on the sample table. The laser energy is initially set to a reference energy.

3. The method for measuring the three-dimensional distribution of a medicine-food composition according to claim 1, characterized in that: In step 2, the optical transceiver includes a second optical transmitter and a signal acquisition device. The signal acquisition device is installed with a bandpass filter. The signal acquisition device receives the fluorescence signal to generate first spectral data. The first spectral data is fluorescence spectral data. When the fluorescence signal drops to the baseline, the first optical transmitter and the optical transceiver are turned off. The first moment is the moment when the first optical transmitter and the optical transceiver are turned off.

4. The method for measuring the three-dimensional distribution of a medicine-food composition according to claim 1, characterized in that: In step 3, the first valve controls the carrier gas to enter the experimental table, the second valve controls the gas molecules to enter the ionization chamber, and the third valve controls the sample ions to enter the mobility chamber. The carrier gas brings the gas molecules into the ionization chamber, and the sample ions are introduced into the mobility chamber through the ion funnel. The carrier gas is helium, the ionization chamber is in negative ion mode, the electrospray contains methanol, water and formic acid, the mass fraction of formic acid is 0.1%, and the volume ratio of methanol and water is 70%:30%. The second moment is the moment when the third valve is opened.

5. The method for measuring the three-dimensional distribution of the edible and medicinal composition according to claim 1, characterized in that: In step 4, the first migration interval is calculated based on the first properties of the target component and the buffer gas, the first flight interval is calculated based on the second properties of the target component and the buffer gas, the sampling interval of the target component is calculated based on the first migration interval and the first flight interval, and then the minimum sampling moment and the maximum sampling moment of the target component are obtained in combination with the second moment and the sampling interval, and sampling data is intercepted from the first ion mobility spectrum based on the minimum sampling moment and the maximum sampling moment.

6. The method for measuring the three-dimensional distribution of the edible and medicinal composition according to claim 5, characterized in that: In step 4, the mobility chamber contains a buffer gas, the buffer gas is helium, the flight channel is a high vacuum environment, the sampling data is the target ion peak, the target component is quercetin, the first attribute is the ion collision cross section, and the second attribute is the mass-to-charge ratio.

7. The method for measuring the three-dimensional distribution of a medicine-food composition according to claim 1, characterized in that: In step 5, the sampling data set includes sampling data under multiple different parameters, and an algorithm parameter set is obtained based on the sampling data set, wherein the algorithm parameter set includes a symmetry factor and a peak width. An objective function is established based on the algorithm parameter set, and the erosion parameters, ionization parameters, and mobility parameters are optimized based on the objective function and the optimization algorithm to obtain target erosion parameters, target ionization parameters, and target mobility parameters. The object to be detected is a medicinal and edible composition.

8. The method for measuring the three-dimensional distribution of a medicine-food composition according to claim 6, characterized in that: In step 7, the center of the light spot of the first light emitter is aligned with the center of the target grid, the first laser is perpendicular to the laboratory table, the optical transceiver is parallel to the laboratory table, the second laser is not in the same plane as the first laser, the minimum sampling time and the maximum sampling time are recalculated, and the target ion peak of the second ion mobility spectrum is intercepted. The content of the target component in the target grid is calculated based on the standard curve and the second ion mobility spectrum.

9. The method for measuring the three-dimensional distribution of a medicine-food composition according to claim 1, characterized in that: In step 9, the target grid is aligned with the two-dimensional data of the composition slice, the two-dimensional data is filled with the content of the target component in the corresponding target grid, and a three-dimensional slice model is generated based on the thickness of the two-dimensional data. Multiple three-dimensional slice models are stacked in sequence to establish a three-dimensional content model of the object to be detected.

10. A measuring device for realizing the method for measuring the three-dimensional distribution of the edible and medicinal composition according to claim 1, characterized in that: include: A laboratory table comprising a glass cover and a sample table, wherein the glass cover maintains a vacuum environment and the sample table holds samples of objects to be tested and slices of the composition; a first light emitter, configured to emit a first laser; An optical transceiver, comprising a second light emitter and a signal acquisition device, wherein the second light emitter is used to emit a second laser, and the signal acquisition device is used to collect a fluorescence signal to generate first spectral data; an ionization chamber, which is used to generate electrospray to ionize gas molecules to produce sample ions; The mobility cell is used to distinguish the target ion from other ions with similar mass-to-charge ratios; The flight channel is used to separate target ions and other ions with different mass-to-charge ratios in sample ions through free flight in a high vacuum environment; The mass analyzer is used to capture sample ions and generate a first ion mobility spectrum and a second ion mobility spectrum; a data processing device configured to calculate a minimum sampling time and a maximum sampling time, intercept a target ion peak from the second ion mobility spectrum, obtain the content of the target component in the target grid according to a standard curve, and establish a three-dimensional content model of the object to be detected; a data analysis device configured to analyze the first moment based on the first spectral data, obtain an algorithm parameter set based on the sampled data set, establish an objective function based on the algorithm parameter set, and optimize the ablation parameter, the ionization parameter, and the mobility parameter based on the objective function and the optimization algorithm; A three-dimensional positioning system is used to obtain two-dimensional data and thickness of a composite slice, divide the target grid, move the first light emitter and the light transceiver according to the target grid, and replace the composite slice to complete the measurement.

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