Method for extracting flavonoid compounds from lonicera caerulea leaves

Abstract

The method comprises the following steps: (1) taking a certain amount of lonicera caerulea leaves, drying the lonicera caerulea leaves at 60 DEG C, grinding the lonicera caerulea leaves, and sieving the lonicera caerulea leaves; (2) adding an ethanol aqueous solution with the concentration of 50% into the lonicera caerulea leaf powder obtained in the step (1) according to the material-liquid ratio of 1: 50 (g / ml), soaking for 60 minutes, extracting for 4 times under the condition of 300W power in combination with an ultrasonic method, combining extracting solutions, and performing suction filtration; (3) performing reduced pressure distillation on the ethanol extracting solution obtained in the step (2), and fixing the volume to 100ml; (4) centrifuging the solution obtained in the step (3), carrying out secondary suction filtration, and fixing the volume to 100 ml; and (5) performing reduced pressure distillation on the solution obtained in the step (4) to obtain paste, thereby obtaining the lonicera caerulea leaf crude extract. The method disclosed by the invention is simple in steps, convenient to operate, short in extraction time, high in extraction rate and few in impurities.

Description

Technical Field

[0001] The invention relates to the technical field of preparation of flavonoid compounds, and in particular to a method for extracting flavonoid compounds from Lonicera caerulea leaves. Background Art

[0002] Blue indigo fruit (Lonicera caerulea), a deciduous shrub of the genus Lonicera in the family Caprifoliaceae, is distributed in Northeast China, Inner Mongolia, Hebei, Shanxi, Ningxia, Gansu, Qinghai, Sichuan and Yunnan. It has rich edible and medicinal value and is one of the important wild plant resources in Northeast my country.

[0003] Flavonoids are secondary metabolites of plants, found widely in green plants, particularly in higher plants such as the Asteraceae, Leguminosae, Rutaceae, Scrophulariaceae, and Lamiaceae families. Flavonoids serve as indicators of rhizobium infection and promote UV protection and anthocyanin formation in plants.

[0004] Currently, the main extraction methods for flavonoids include supercritical fluid extraction, pressurized solvent extraction, pulsed electric field-assisted extraction, and enzyme-assisted extraction. However, these methods have disadvantages such as complex processes, high costs, and high levels of residual organic solvents. Therefore, the search for a more convenient, rapid, safe, and environmentally friendly extraction method has become a research hotspot. Summary of the Invention

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a method for extracting flavonoids from Lonicera caerulea leaves.

[0006] The technical solution of the present invention is summarized as follows:

[0007] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0008] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0009] (2) adding 50% ethanol aqueous solution to the blue loquat leaf powder prepared in step (1) at a solid-liquid ratio of 1:50 (g / ml), soaking for 60 minutes, and extracting four times with ultrasonic method at a power of 300 W. The extracts were combined and filtered;

[0010] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0011] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0012] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0013] Furthermore, after the grinding treatment in step (1), the product is sieved using a 60-mesh sieve.

[0014] Furthermore, the volume concentration of the ethanol aqueous solution in step (2) is 40-70%.

[0015] Furthermore, the material-liquid ratio in step (2) is 1:30 to 1:60 (g / ml).

[0016] Furthermore, in step (2), the power of ultrasonic extraction is 200W to 500W, and the extraction time is 30 to 50 minutes.

[0017] Furthermore, the number of ultrasonic extractions in step (2) is 1 to 4 times.

[0018] The advantages of the present invention are that, compared with the traditional extraction method, the ultrasonic extraction method has simple steps, convenient operation, short extraction time of only 30 minutes, high extraction rate, few impurities, and good practicality. DETAILED DESCRIPTION

[0019] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific embodiments.

[0020] Example 1

[0021] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0022] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0023] (2) adding a 60% ethanol aqueous solution to the Lonicera edulis leaf powder prepared in step (1) at a solid-liquid ratio of 1:30 (g / ml), soaking for 60 minutes, and extracting three times with an ultrasonic method at a power of 400 W. The extracts were combined and filtered;

[0024] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0025] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0026] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0027] Example 2

[0028] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0029] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0030] (2) adding 40% ethanol aqueous solution to the blue loquat leaf powder prepared in step (1) at a solid-liquid ratio of 1:40 (g / ml), soaking for 60 minutes, and extracting four times with ultrasonic method at a power of 300 W. The extracts were combined and filtered;

[0031] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0032] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0033] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0034] Example 3

[0035] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0036] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0037] (2) adding 50% ethanol aqueous solution to the blue loquat leaf powder prepared in step (1) at a solid-liquid ratio of 1:50 (g / ml), soaking for 60 minutes, and extracting once with ultrasonic method at a power of 500 W. The extracts were combined and filtered;

[0038] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0039] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0040] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0041] Example 4

[0042] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0043] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0044] (2) adding 70% ethanol aqueous solution to the blue loquat leaf powder prepared in step (1) at a solid-liquid ratio of 1:60 (g / ml), soaking for 60 minutes, and extracting twice with ultrasonic method at a power of 200 W. The extracts were combined and filtered;

[0045] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0046] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0047] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0048] Example 5

[0049] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0050] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0051] (2) adding 70% ethanol aqueous solution to the blue loquat leaf powder prepared in step (1) at a solid-liquid ratio of 1:30 (g / ml), soaking for 60 minutes, and extracting four times with ultrasonic method at a power of 500 W. The extracts were combined and filtered;

[0052] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0053] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0054] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0055] Example 6

[0056] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0057] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0058] (2) adding 50% ethanol aqueous solution to the blue loquat leaf powder prepared in step (1) at a solid-liquid ratio of 1:40 (g / ml), soaking for 60 minutes, and extracting three times with ultrasonic method at a power of 200 W. The extracts were combined and filtered;

[0059] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0060] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0061] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0062] Example 7

[0063] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0064] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0065] (2) adding 40% ethanol aqueous solution to the blue loquat leaf powder prepared in step (1) at a solid-liquid ratio of 1:40 (g / ml), soaking for 60 minutes, and extracting twice with ultrasonic method at a power of 400 W. The extracts were combined and filtered;

[0066] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0067] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0068] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0069] Example 8

[0070] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0071] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0072] (2) adding 50% ethanol aqueous solution to the blue loquat leaf powder prepared in step (1) at a solid-liquid ratio of 1:60 (g / ml), soaking for 60 minutes, and extracting four times with ultrasonic method at a power of 500 W. The extracts were combined and filtered;

[0073] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0074] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0075] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0076] Comparative Example 1

[0077] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0078] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0079] (2) adding a 40% ethanol aqueous solution to the Lonicera edulis leaf powder prepared in step (1) at a material-liquid ratio of 1:30 (g / ml), soaking for 60 minutes, and extracting once with ultrasonic method at a power of 200 W. The extracts were combined and filtered;

[0080] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0081] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0082] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0083] Comparative Example 2

[0084] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0085] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0086] (2) adding 70% ethanol aqueous solution to the blue loquat leaf powder prepared in step (1) at a solid-liquid ratio of 1:50 (g / ml), soaking for 60 minutes, and extracting three times with ultrasonic method at a power of 300 W. The extracts were combined and filtered;

[0087] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0088] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0089] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0090] Comparative Example 3

[0091] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0092] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0093] (2) adding 50% ethanol aqueous solution to the blue loquat leaf powder prepared in step (1) at a solid-liquid ratio of 1:30 (g / ml), soaking for 60 minutes, and extracting twice with ultrasonic method at a power of 300 W. The extracts were combined and filtered;

[0094] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0095] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0096] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0097] Comparative Example 4

[0098] A method for extracting flavonoids from Lonicera caerulea leaves comprises the following steps:

[0099] (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them;

[0100] (2) adding a 60% ethanol aqueous solution to the Lonicera edulis leaf powder prepared in step (1) at a solid-liquid ratio of 1:60 (g / ml), soaking for 60 minutes, and extracting once with ultrasonication at a power of 300 W. The extracts were combined and filtered;

[0101] (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml;

[0102] (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml;

[0103] (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

[0104] Table 1 Contents of flavonoids in the leaves of Lonicera edulis

[0105]

[0106] The above illustrates and describes the basic principles, main features, and advantages of the present invention. The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for extracting flavonoids from Lonicera caerulea leaves, characterized by: The preparation method comprises the following steps: (1) Taking a certain amount of blue indigo carica leaves, drying them at 60°C, grinding them, and sieving them; (2) adding 50% ethanol aqueous solution to the blue loquat leaf powder prepared in step (1) at a solid-liquid ratio of 1:50 (g / ml), soaking for 60 minutes, and extracting four times with ultrasonic method at a power of 300 W. The extracts were combined and filtered; (3) distilling the ethanol extract obtained in step (2) under reduced pressure to a volume of 100 ml; (4) The solution obtained in step (3) was centrifuged and filtered twice and the volume was adjusted to 100 ml; (5) The solution obtained in step (4) is distilled under reduced pressure into a paste to obtain a crude extract of Lonicera caerulea leaves.

2. The method for extracting flavonoids from Lonicera caerulea leaves according to claim 1, wherein: After the grinding process, the mixture was sieved using a 60-mesh sieve.

3. The method for extracting flavonoids from Lonicera caerulea leaves according to claim 1, wherein: The volume concentration of the ethanol aqueous solution is 40-70%.

4. The method for extracting flavonoids from Lonicera caerulea leaves according to claim 1, wherein: The material-liquid ratio is 1:30 to 1:60 (g / ml).

5. The method for extracting flavonoids from Lonicera caerulea leaves according to claim 1, wherein: The power of the ultrasonic extraction is 200W to 500W, and the extraction time is 30 to 50 minutes.

6. The method for extracting flavonoids from Lonicera caerulea leaves according to claim 1, wherein: The number of ultrasonic extractions is 1 to 4 times.

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