Rat fixing device

Abstract

The invention relates to the technical field of animal experiments, and discloses a rat fixing device which comprises a plurality of arc-shaped fixing plates and a plurality of limiting assemblies, the limiting assemblies are connected with the arc-shaped fixing plates in a one-to-one correspondence mode, the arc-shaped fixing plates are arranged in a mirror image mode, and the adjacent arc-shaped fixing plates located on the same side are connected through length adjusting assemblies. The length adjusting assembly adjusts the distance between the arc-shaped fixing plates located on the same semicircular side in the horizontal direction, the limiting assembly comprises an adjusting screw rod and a base plate, the adjusting screw rod penetrates through the arc-shaped fixing plates and is in threaded connection with the arc-shaped fixing plates, and the base plate is fixedly connected with the end, located on the inwards-concave sides of the arc-shaped fixing plates, of the adjusting screw rod. According to the rat fixing device, rats of different growth stages and strains can be fixed through a single device.

Description

Technical Field

[0001] The invention relates to the technical field of animal experiments, in particular to a rat fixing device. Background Art

[0002] In basic medical fields like neuroscience, pharmacology, and physiology, rats are commonly used as experimental animals. Their immobilization is crucial for much more than simply ensuring smooth procedures. A scientifically sound immobilization method minimizes stress responses caused by the rat's struggles. Excessive stress can lead to abnormal fluctuations in hormone levels, which can interfere with various physiological parameters, such as neurotransmitter release and immune function, potentially compromising the accuracy of experimental data. The choice of immobilization method must be precisely tailored to each experimental scenario. For example, in brain surgery, in addition to conventional body immobilization, procedures like cranial drilling and microinjections must be precisely targeted to the brain region to avoid experimental errors caused by minor head movements. In long-term electrophysiological recording experiments, the immobilization device must also take into account the rat's freedom of movement. Lightweight designs or flexible wire connections can be used to minimize interference with the rat's natural behavior, thereby obtaining more physiologically accurate neural discharge signals. In pharmacological research, the stability of the immobilization method is directly related to the accuracy of drug delivery. Taking intravenous injection as an example, if the fixation is unstable and causes the needle to shift, it may not only cause drug extravasation and cause local tissue damage, affecting the drug absorption rate, but also cause the actual dosage to deviate from the preset value, thereby affecting the accuracy of the dose-response curve; during gavage operation, stable fixation can help experimenters more smoothly insert the gavage needle along the esophagus into the stomach, reducing the risk of suffocation caused by accidental insertion into the trachea, while ensuring that all the drug solution enters the stomach, avoiding drug loss due to animal vomiting. In the rat toe inflammation and swelling method experiment, stable fixation is the prerequisite for ensuring that the drug solution is accurately injected into the target area. If the fixation is not firm, the rat will struggle due to pain or discomfort, which may cause the injection needle to deviate from the preset subcutaneous position of the paw, resulting in uneven distribution of the inflammatory agent and varying intensity of the inflammatory response. The subsequent measurement of the paw swelling degree will show large individual differences, affecting the consistency of the experiment.

[0003] Existing fixation devices use curved fixing plates that open and close left and right to fix rats. The size is often fixed and can only be used for rats of a specific size. It cannot meet the fixation needs of mice at different growth stages or different breeds. It is difficult for experimenters to easily place rats, and it cannot be adjusted according to individual differences of rats. The fixation often results in problems such as being too loose, causing the rat to struggle and affect the experiment, or being too tight, causing oppression on the rat. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention provides a rat fixation device, which can fix rats of different growth stages and strains with a single device.

[0005] An embodiment of the present invention provides a rat fixation device, comprising: a plurality of arc-shaped fixing plates and a plurality of limiting components, wherein the plurality of limiting components are connected to one arc-shaped fixing plate in a one-to-one correspondence; Multiple arc-shaped fixing plates are arranged in a mirror image, and multiple adjacent arc-shaped fixing plates on the same side are connected by a length adjustment component, and the length adjustment component adjusts the horizontal spacing of the multiple arc-shaped fixing plates on the same semicircle side; The limiting assembly includes an adjusting screw and a pad. The adjusting screw passes through the arc-shaped fixing plate and is threadedly connected to the arc-shaped fixing plate. The pad is fixedly connected to one end of the adjusting screw located on the concave side of the arc-shaped fixing plate.

[0006] Optionally, the first sides of the two left and right mirror-image arc-shaped fixing plates are hinged, and the second sides are detachably connected.

[0007] Optionally, the corresponding multiple arc-shaped fixing plates on the left and right are provided with mutually meshing tooth structures, and the tooth structures are provided with recessed holes. After the tooth structures are meshed, fixing rods are inserted into the recessed holes, and the fixing rods limit the circumferential movement of the arc-shaped fixing plates.

[0008] Optionally, it also includes a glass cover and a limiting strip. The top of the arc-shaped fixing plate at the top is provided with a groove, and the bottom of the glass cover has a convex strip matching the groove. The convex strip and the groove are embedded in each other. The first end of multiple limiting strips is connected to the arc-shaped fixing plate, and the second end is detachably connected to the glass cover.

[0009] Optionally, it also includes multiple box bodies, which are fixed to the lower ends of two mirror-arc fixed plates. A drawer is slidably connected inside the box body, and a Y-shaped fixing frame and an ultraviolet lamp are fixed in the drawer. The vertical part of the Y-shaped fixing frame is a telescopic rod. The Y-shaped fixing frame is connected to the heat source, and the ultraviolet lamp is fixed to one side of the Y-shaped fixing frame.

[0010] Optionally, the two length adjustment components include a slide rail and a slider, the slide rail is connected to the upper half of the box body, the slide rail is slidably connected to the slider, and one end of the slider along the sliding direction is connected to the lower half of the box body.

[0011] Optionally, it further includes Velcro, and both ends of the Velcro are detachably connected to two adjacent arc-shaped fixing plates located on the same side.

[0012] Optionally, a foot fixing groove is provided at the bottom of the arc-shaped fixing plate away from the glass cover.

[0013] Optionally, a head limiting assembly is also included, which includes two limiting half rings and an adjusting rod. The adjusting rod includes two connecting rods hinged to each other. The first end of the adjusting rod is fixedly connected to the box body, and the second end is hinged to the first ends of the two limiting half rings. The first ends of the two limiting half rings are rotatably connected, and the second ends are detachably connected through a connecting piece.

[0014] Optionally, a pad and a second anti-slip pad are connected inside the two limiting half rings.

[0015] The technical solution provided by the embodiments of the present invention has the following advantages over the prior art: The embodiments of the present invention provide a rat fixation device, comprising an arc-shaped fixing plate and a plurality of limiting components. The arc-shaped fixing plate is adjusted in length by a length adjustment component between two adjacent arc-shaped fixing plates. The adjustment screw can radially adjust the movement of the pad to accommodate differences in the rat's chest and abdomen, such as shorter lengths for young rats and longer lengths for adult rats. The device can be adjusted in two dimensions: length and radial tightness, enabling a single device to cover rats of different growth stages and strains, combining the advantages of versatility, ease of operation, and improved experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A schematic perspective view of a rat fixation device provided by an embodiment of the present invention; Figure 2 A side view of a rat fixation device provided by an embodiment of the present invention; Figure 3 A front view of a rat fixation device provided by an embodiment of the present invention; Figure 4 A schematic structural diagram of a fixed half ring provided in an embodiment of the present invention; Figure 5 A schematic structural diagram of a glass sleeve provided in an embodiment of the present invention; Figure 6 A schematic structural diagram of a head limiting assembly provided in an embodiment of the present invention.

[0017] Description of reference numerals: 1. Box body; 2. Arc-shaped fixing plate; 3. Length adjustment assembly; 31. Velcro; 4. Limit assembly; 5. Limit strip; 11. Y-shaped fixing frame; 12. UV lamp; 23. Glass cover; 41. Adjusting screw; 42. Pad; 24. Tooth structure; 241. Fixing rod; 242. Recessed hole; 25. Groove; 231. Raised strip; 10. Drawer; 13. Buckle; 101. Buttonhole; 14. Foot fixing groove; 43. First anti-slip pad; 6. Head limit assembly; 61. Limiting half ring; 62. Adjusting rod; 611. Pad; 612. Second anti-slip pad; 32. Slide rail; 33. Slider. DETAILED DESCRIPTION

[0018] A specific embodiment of the present invention is described in detail below with reference to the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific embodiment.

[0019] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "axial", "radial", "circumferential" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the technical solutions of the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0020] The present invention is described below by means of several specific embodiments. To keep the following description of the embodiments of the present invention clear and concise, detailed descriptions of known functions and components may be omitted. When any component of an embodiment of the present invention appears in more than one drawing, the component may be represented by the same reference numeral in each drawing.

[0021] Figure 1 This is a three-dimensional schematic diagram of a rat fixation device provided by an embodiment of the present invention. Figure 2 A side view of a rat fixation device provided by an embodiment of the present invention, Figure 3 This is a front view of a rat fixation device provided by an embodiment of the present invention. Figure 4 A schematic diagram of the structure of a fixed half ring provided in an embodiment of the present invention is shown. Figure 5 A schematic diagram of the structure of the glass sleeve provided in an embodiment of the present invention. Figure 6 A schematic structural diagram of a head limiting assembly provided in an embodiment of the present invention.

[0022] like Figure 1 and Figure 2 As shown, an embodiment of the present invention provides a rat fixation device, comprising: a plurality of arc-shaped fixing plates 2 and a plurality of limiting assemblies 4, wherein the plurality of limiting assemblies 4 are connected to a single arc-shaped fixing plate 2 in a one-to-one correspondence, and the plurality of arc-shaped fixing plates 2 are arranged in a mirror image, and a plurality of adjacent arc-shaped fixing plates 2 located on the same side are connected via a length adjustment assembly 3, and the length adjustment assembly 3 adjusts the horizontal spacing of the plurality of arc-shaped fixing plates 2 located on the same semicircular side; the limiting assembly 4 comprises an adjusting screw 41 and a pad 42, the adjusting screw 41 passes through the arc-shaped fixing plate 2 and is threadedly connected to the arc-shaped fixing plate 2, and the pad 42 is fixedly connected to one end of the adjusting screw 41 located on the concave side of the arc-shaped fixing plate 2.

[0023] Specifically, the curved fixing plate 2 has a screw hole that engages with the adjusting screw 41. The backing plate 42 is a curved plate with a first anti-slip pad 43 internally connected to it. The elastic properties of the first anti-slip pad 43 adapt to the thoracic curves of rats of different sizes, avoiding the respiratory restriction caused by traditional rigid fixation. The surface micro-texture increases the friction coefficient without damaging the skin, making it particularly suitable for chronic experiments requiring long-term fixation.

[0024] An embodiment of the present invention provides a rat fixation device comprising a split-design curved fixing plate 2, a length adjustment assembly 3, and a limiting assembly 4. The curved fixing plate 2 is a split structure with mirror symmetry between the left and right sides. Multiple adjacent curved fixing plates 2 are connected by the length adjustment assembly 3, allowing for horizontal adjustment of spacing to accommodate rats of varying lengths, such as shortening for young rats and lengthening for adult rats. The limiting assembly 4 comprises an adjusting screw 41 and a curved pad 42. The adjusting screw 41 is threadedly engaged with the curved fixing plate 2. The curved pad 42 is disposed at the end of the adjusting screw 41 and is lined with an elastic non-slip pad. This pad 42 is radially adjustable to accommodate differences in chest and abdominal thickness in rats.

[0025] Optional, reference Figure 1 The first sides of the two left and right mirror-image arc-shaped fixing plates 2 are hinged, and the second sides are detachably connected.

[0026] The first side hinge of the arc-shaped fixed plate 2, which is mirrored on the left and right, is connected by a hinge hinge. The hinge axis coincides with the center of curvature of the arc-shaped plate. During opening and closing, the hinge side serves as a rotation fulcrum.

[0027] Optional, reference Figure 1 The corresponding multiple arc-shaped fixing plates 2 on the left and right are provided with mutually meshing tooth structures 24, and the tooth structures 24 are provided with recessed holes 242. After the tooth structures 24 are meshed, the fixing rods 241 are inserted into the recessed holes 242, and the fixing rods 241 limit the circumferential movement of the arc-shaped fixing plates 2.

[0028] After the tooth-like structures 24 are engaged, recessed holes 242 are selected based on the size of the rat, and fixed with a fixing rod 241 to prevent the left and right fixing half-rings 21 and 22 from separating and potentially escaping. Furthermore, the different recessed holes 242 accommodate different rats. This device, with its three-dimensional adjustment of length, radial direction, and tightening tension, allows a single device to accommodate rats of different growth stages and strains, offering advantages such as versatility, ease of operation, and improved experimental efficiency.

[0029] During experimental activities on rats, the head often needs to be processed and observed, and the glass cover 23 on the head of the arc-shaped fixing plate 2 needs to be frequently removed. Therefore, the embodiment of the present invention provides a simple disassembly structure of the glass cover 23 and the arc-shaped fixing plate 2.

[0030] Optional, reference Figure 3 and Figure 5 , also includes a glass sleeve 23 and a limiting strip 5. A groove 25 is provided at the top of the arc-shaped fixing plate 2 at the top. A convex strip 231 matching the groove 25 is provided at the bottom of the glass sleeve 23. The convex strip 231 and the groove 25 are embedded in each other. The first end of multiple limiting strips 5 is detachably connected to the arc-shaped fixing plate 2, and the second end is detachably connected to the glass sleeve 23.

[0031] In an embodiment of the present invention, a groove 25 is provided at the top of the arc-shaped fixing plate 2, which forms a precise interlocking structure with the convex strip 231 at the bottom of the glass sleeve 23, thereby achieving rapid positioning and a stable connection. This design has the advantages of precise anti-drifting, enhanced sealing, and easy and quick disassembly and assembly. It can not only ensure the stability of the glass sleeve 23 during the experiment, but also prevent the experimenter from being bitten, ensuring the safety and reliability of the experiment, and effectively isolate external interference. It is suitable for scenarios requiring environmental control such as neurobehavioral and pharmacological experiments. At the same time, the modular interlocking design supports the rapid replacement of different functional covers, such as transparent observation covers, opaque experimental covers, or special covers with sensor interfaces, to meet the diverse needs of injection operations, image monitoring, long-term experiments, etc., significantly improving the experimental efficiency and device reliability. The two ends of the limiting strip 5 are fixed with glue to connect to the arc-shaped fixing plate 2 and the glass sleeve 23, ensuring that the glass sleeve 23 is firmly covered and easy to observe.

[0032] In experiments on rats, intravenous injection needs to be performed on the rat's tail. After the rat is fixed, its tail also needs to be fixed to facilitate the injection. Therefore, an embodiment of the present invention provides an improved method.

[0033] Optional, reference Figure 1 , and also includes multiple box bodies 1, the box bodies 1 are fixed to the lower ends of two mirror-arc fixed plates 2, and are located in the box body 1 and are slidably connected to a drawer 10. A Y-shaped fixing frame 11 and an ultraviolet lamp 12 are fixed in the drawer 10. The vertical part of the Y-shaped fixing frame 11 is a telescopic rod. The Y-shaped fixing frame 11 is connected to a heat source, and the ultraviolet lamp 12 is fixed to one side of the Y-shaped fixing frame 11.

[0034] Specifically, the hinge is fixedly connected to the box body 1 to provide reliable support for the arc-shaped fixing plate 2.

[0035] The embodiment of the present invention further optimizes the rat tail fixation solution. By arranging a sliding drawer 10 in the box body 1 and integrating a Y-shaped fixing frame 11 and an ultraviolet lamp 12, the operational convenience of tail vein injection is significantly improved. The telescopic rod design of the Y-shaped fixing frame 11 can adapt to rat tails of different lengths and promote blood vessel dilation in conjunction with the heat source; the front ultraviolet lamp 12 can assist in locating the vein and quickly disinfect the injection site to avoid cross infection. The drawer 10 structure not only maintains the integrity of the device, but also can quickly unfold the tail operation module, allowing the experimenter to complete the entire process of tail fixation-disinfection-injection with one hand, greatly improving the efficiency of the experiment. This design is particularly suitable for long-term pharmacological experiments that require frequent tail vein injections. While ensuring the stability of the rat body, it realizes the specialization and standardization of tail operations.

[0036] When the present invention is used in a specific experiment, it may be necessary to hold the entire device vertically for observation. In this case, the drawer 10 may slide out, causing unnecessary trouble. Therefore, an embodiment of the present invention provides an improved method.

[0037] Specifically, a buttonhole 101 is fixed on the outside of the drawer 10, and a buckle head 13 matching the buttonhole 101 is fixed on the box body 1, and the buckle head 13 is snap-fitted with the buttonhole 101. A buttonhole 101 is added to the outside of the drawer 10, and a matching buckle head 13 is set at the corresponding position of the box body 1. When the device needs to be upright or tilted, it is only necessary to snap-fit ​​and fix the buckle head 13 with the buttonhole 101 to effectively prevent the drawer 10 from sliding out accidentally, ensuring the continuity and safety of the experimental operation. This improved design not only retains the convenience of the sliding structure of the drawer 10, but also increases the stability during use. It is particularly suitable for complex experimental scenarios that require changing the angle of the device, allowing researchers to focus more on the experimental operation itself.

[0038] Optional, reference Figure 3 The two length adjustment components 3 include a slide rail 32 and a slider 33. The slide rail 32 is connected to the upper half of the box body 1. The slide rail 32 is slidably connected to the slider 33. One end of the slider 33 along the sliding direction is connected to the lower half of the box body 1.

[0039] Through the cooperation of the slide rail 32 and the slider 33, the length of the arc-shaped fixed plate 2 can be adjusted to adapt to rats of different lengths. For example, a short length is required for young rats, and a long length is required for adult rats. After adjustment, it is fixed by the Velcro 31 to ensure that the rats do not curl up or overstretch, which significantly improves the versatility of the device. A single device can cover rats of different growth stages and different strains.

[0040] Optional, reference Figure 3 , and also includes a Velcro 31, the two ends of the Velcro 31 are detachably connected to two adjacent arc-shaped fixing plates 2 located on the same side.

[0041] The distance between two adjacent arc-shaped fixing plates 2 is adjusted and fixed by Velcro 31. The two ends of Velcro 31 are fixed with adhesive to the arc-shaped fixing plates 2 to ensure the natural posture of the rat and prevent part of the rat's body from extending outside the two arc-shaped fixing plates 2 when the distance between two adjacent arc-shaped fixing plates 2 is too large.

[0042] In the above-mentioned embodiment of the invention, the feet of the rats are not fixed. When the rats are subjected to experimental activities, they may be stimulated. After being stimulated, the rats' feet may move around, which may affect the experiment. Therefore, the embodiment of the present invention provides an improved method.

[0043] Optional, reference Figure 3 A foot fixing groove 14 is provided at the bottom of the arc-shaped fixing plate 2 away from the glass sleeve 23.

[0044] The foot fixing groove 14 is an inverted U-shaped structure.

[0045] The present embodiment addresses the issue of rat foot fixation by providing a dedicated foot fixation groove 14 at the bottom of the curved fixation plate 2. This design utilizes a curved groove 25 structure that matches the naturally extended position of the rat's limbs, achieving gentle and effective foot restraint without affecting blood circulation. Foot fixation groove 14 is lined with a soft silicone material, providing a comfortable contact surface to prevent skin damage while also generating moderate friction to prevent slippage. This improvement significantly enhances experimental stability and effectively eliminates experimental interference caused by the rat's limb movement.

[0046] After removing the glass cover 23 on the head, when conducting experimental activities on the mouse head, the mouse head needs to be fixed again to prevent it from biting the experimenter. Therefore, the embodiment of the present invention provides an improved method.

[0047] Optional, reference Figure 3 and Figure 6 , and also includes a head limiting component 6, the head limiting component 6 includes two limiting semi-rings 61 and an adjusting rod 62, the adjusting rod 62 includes two connecting rods hinged to each other, the first end of the adjusting rod 62 is fixedly connected to the box body 1, and the second end is hinged to the first ends of the two limiting semi-rings 61, the first ends of the two limiting semi-rings 61 are rotatably connected, and the second ends are detachably connected through a connecting piece.

[0048] The ends of the two connecting rods are connected by a pin, wherein the end of the first connecting rod is provided with a U-shaped fork, and the corresponding end of the second connecting rod is embedded in the fork and aligned through a through hole. After the stainless steel pin is inserted, the two ends are fixed with cotter pins. This design allows the connecting rod to swing ±30° around the pin axis while bearing tensile and compressive loads in the axial direction. Nylon bushings are also installed at the hinge to reduce friction, allowing the adjustment rod 62 to adapt to angle changes during the extension and retraction process. The first end of the two limiting half rings 61 is provided with a hinge ear with an axis hole at the end of one of the limiting half rings 61, and a matching hinge axis is provided at the corresponding position of the other limiting half ring 61. The two are fixed by a stainless steel pin or rivet, so that the limiting half ring 61 can rotate relative to each other around the pin. The second end is detachably connected by a magnet.

[0049] The present embodiment innovatively features an adjustable head restraint assembly 6 to address safety concerns during head experiments. This assembly utilizes an opening and closing structure consisting of two restraint half-rings 61. This assembly allows for rapid opening and closing, achieved through a pivoting connection at the first end and a detachable connection at the second end. The restraint angle can be flexibly adjusted using an articulated adjustment lever 62. One end of the adjustment lever 62 is fixed to the housing 1, while the other end is hinged to the restraint half-ring 61. This ensures a secure head fixation and allows for multiple angle adjustments to accommodate diverse experimental requirements. This prevents the rat from struggling violently during subsequent blood collection and prevents respiratory distress, ensuring safe operation and smooth blood collection. The combined structure facilitates one-handed operation by the experimenter, allowing for quick head fixation after removing the glass cover 23. The articulated adjustment lever 62 allows for precise control of the rat's head elevation angle, meeting the delicate requirements of procedures such as brain injections and electrode implantation. This improvement is particularly suitable for neuroscience experiments requiring head exposure, significantly enhancing experimental controllability and reproducibility.

[0050] Optional, reference Figure 6 The two limiting half rings 61 are internally connected with a pad 611 and a second anti-slip pad 612 .

[0051] The embodiment of the present invention has made further optimizations in the humanized design of the head limiting assembly 6, and innovatively set a composite protection system on the inner side of the two limiting half rings 61. The system adopts a double-layer structural design: the inner layer is a medical silicone liner 611, which has moderate elasticity and biocompatibility to ensure comfort when in contact with the rat's head; the outer layer is a special second anti-slip pad 612, which adopts a micro-convex texture surface treatment to significantly increase the friction coefficient. The double-layer combination can not only effectively prevent the rat's head from sliding and shifting, but also avoid the skin compression damage that may be caused by traditional fixation methods. The anti-slip design is particularly suitable for experiments that require precise positioning of the head, and can ensure the stability of the head position during the experiment. The liner 611 adopts a detachable design, which is easy to disinfect and replace, and meets the hygiene requirements of different experimental rooms. This optimized design enables the device to ensure experimental accuracy while being particularly suitable for experimental scenarios that require long-term head fixation.

[0052] The present invention provides two specific applications of the present invention in rat experiments: Experiment 1, tail vein injection experiment: After the length adjustment component 3 adjusts the two adjacent arc-shaped fixing plates 2 to the appropriate length, the tooth-shaped structures 24 engage with each other for preliminary fixation, and then the limiting component 4 is adjusted to fix the rat for the second time; Then pull out the drawer 10, adjust the Y-shaped fixing frame 11 to a suitable height, clamp the rat's tail on the Y-shaped fixing frame 11, turn on the ultraviolet lamp 12 to irradiate the tail, and at the same time, the heat source heats the Y-shaped fixing frame 11 to assist the injection.

[0053] After the injection is completed, the heat source is turned off, the mouse's tail is removed, the ultraviolet lamp 12 is turned off, and the Y-shaped fixing frame 11 and the drawer 10 are restored to their original positions.

[0054] Experiment 2, eye blood sampling experiment: the fixation method is the same as Experiment 1; After the fixation is completed, the button head 13 buttonhole 101 is snapped in place to prevent the drawer 10 from accidentally sliding out. The entire device is set vertically, the head glass cover 23 is removed, and the head limit assembly 6 is adjusted to a suitable position to fix the mouse's head. Ensure that during the subsequent blood collection operation, the rat neither struggles violently nor compresses the airway. Confirm that the respiratory rate is normal to ensure the safety of the operation and the smooth progress of blood collection. Gently lift the rat's head to fully expose the eyeball. Use ophthalmic tweezers or a capillary to gently touch the posterior fornix of the eyeball to induce bleeding; collect blood into a centrifuge tube. After completing the blood collection, press the eye with a sterile cotton ball to stop the bleeding;

[0055] After the test is completed, the head limiting assembly 6 is disassembled and the glass cover 23 is reinstalled.

[0056] Experiment 3, rat paw swelling measurement experiment: The fixation method is the same as Experiment 1, and then the rat's foot is fixed in the foot fixing groove 14; After the fixation is completed, the button head 13 buttonhole 101 is snapped in place to prevent the drawer 10 from accidentally sliding out, and the entire device is set vertically; Use a vernier caliper or a special swelling meter to measure the thickness or circumference along the widest part of the plantar fossa and record the experimental data; After the experiment is completed, the entire device is restored to a horizontal position, and the button head 13 and the buttonhole 101 are untied.

[0057] The above inventions are only several specific embodiments of the present invention. However, the embodiments of the present invention are not limited thereto. Any changes that can be conceived by those skilled in the art should fall within the scope of protection of the present invention.

Claims

1. A rat fixing device, characterized in that: It comprises: a plurality of arc-shaped fixing plates (2) and a plurality of limiting components (4), wherein the plurality of limiting components (4) are connected to one arc-shaped fixing plate (2) in a one-to-one correspondence; The plurality of arc-shaped fixing plates (2) are arranged in a mirror image, and the plurality of adjacent arc-shaped fixing plates (2) located on the same side are connected via a length adjustment component (3), and the length adjustment component (3) adjusts the horizontal spacing of the plurality of arc-shaped fixing plates (2) located on the same semicircular side; The limiting assembly (4) comprises an adjusting screw (41) and a backing plate (42); the adjusting screw (41) passes through the arc-shaped fixing plate (2) and is threadedly connected to the arc-shaped fixing plate (2); the backing plate (42) is fixedly connected to one end of the adjusting screw (41) located on the concave side of the arc-shaped fixing plate (2).

2. The rat fixing device according to claim 1, wherein The first sides of the two left and right mirror-image arc-shaped fixing plates (2) are hinged, and the second sides are detachably connected.

3. The rat fixing device according to claim 2, wherein: The plurality of arc-shaped fixing plates (2) corresponding to the left and right sides are provided with mutually meshing tooth structures (24), and the tooth structures (24) are provided with recessed holes (242). After the tooth structures (24) are meshed, a fixing rod (241) is inserted into the recessed hole (242), and the fixing rod (241) limits the circumferential movement of the arc-shaped fixing plates (2).

4. The rat fixing device according to claim 1, wherein The invention also includes a glass sleeve (23) and a limiting strip (5), wherein a groove (25) is provided at the top end of the arc-shaped fixing plate (2), and a convex strip (231) matching the groove (25) is provided at the bottom of the glass sleeve (23), wherein the convex strip (231) and the groove (25) are interlocked, and a plurality of limiting strips (5) are connected to the arc-shaped fixing plate (2) at the first end and detachably connected to the glass sleeve (23) at the second end.

5. The rat fixing device according to claim 1, wherein: The invention also includes a plurality of box bodies (1), wherein the box bodies (1) are fixed to the lower ends of two mirror-image arc-shaped fixing plates (2), and a drawer (10) is slidably connected to the box bodies (1). A Y-shaped fixing frame (11) and an ultraviolet lamp (12) are fixed in the drawer (10), and the vertical part of the Y-shaped fixing frame (11) is a telescopic rod. The Y-shaped fixing frame (11) is connected to a heat source, and the ultraviolet lamp (12) is fixed to one side of the Y-shaped fixing frame (11).

6. The rat fixing device according to claim 5, wherein: The two length adjustment components (3) include a slide rail (32) and a slider (33), wherein the slide rail (32) is connected to the upper half of the box body (1), the slide rail (32) is slidably connected to the slider (33), and one end of the slider (33) along the sliding direction is connected to the lower half of the box body (1).

7. The rat fixing device according to claim 1, wherein: It also includes a Velcro (31), with two ends of the Velcro (31) being detachably connected to two adjacent arc-shaped fixing plates (2) located on the same side.

8. The rat fixing device according to claim 4, wherein: A foot fixing groove (14) is provided at the bottom of the arc-shaped fixing plate (2) away from the glass sleeve (23).

9. The rat fixing device according to claim 5, wherein: The head limiting assembly (6) is further included. The head limiting assembly (6) includes two limiting half rings (61) and an adjusting rod (62). The adjusting rod (62) includes two connecting rods hinged to each other. The first end of the adjusting rod (62) is fixedly connected to the box body (1), and the second end is hinged to the first ends of the two limiting half rings (61). The first ends of the two limiting half rings (61) are rotatably connected, and the second ends are detachably connected through a connecting piece.

10. The rat fixing device according to claim 9, wherein The two limiting half rings (61) are internally connected with a liner (611) and a second anti-slip pad (612).

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