Application of hydroxyl hydrophobic deep-eutectic solvent in extraction of quinine

Abstract

The invention relates to the technical field of eutecticevaporate solvents, in particular to application of a hydroxyl hydrophobic eutecticevaporate solvent in quinine extraction. The method comprises the following steps: placing a hydroxyl hydrophobic deep-eutectic solvent in a quinine-containing aqueous solution, and extracting quinine under the conditions of constant temperature and stirring; the structural formula of the hydroxyl hydrophobic deep-eutectic solvent is shown in the specification. The hydroxyl hydrophobic eutecticevaporate solvent prepared by the preparation method disclosed by the invention has relatively strong trapping capability on quinine; meanwhile, the eutectic solvent has good phase forming ability, and phase separation can be achieved with a small dosage. Under the conditions that the volume ratio of the hydroxyl hydrophobic deep-eutectic solvent to the quinine aqueous solution is 1: 80 and the pH value is 10, the recovery efficiency of quinine is as high as 97.73%. The hydroxyl hydrophobic deep-eutectic solvent extraction agent is high in separation efficiency, environmentally friendly and low in price. # imgabs0 #

Description

Technical Field

[0001] The present invention relates to the technical field of deep eutectic solvents, and in particular to application of a hydroxyl hydrophobic deep eutectic solvent in extracting quinine. Background Art

[0002] Quinine has high biological activity and is widely used in food, medicine, and other fields. Traditionally, quinine is recovered from aqueous phases using organic solvents as extractants. However, these organic solvents are volatile, flammable, and non-degradable, and the extraction process can cause environmental pollution and potential risks to human health. Therefore, the development of environmentally friendly and efficient solvents for the recovery of quinine from aqueous phases is of great significance.

[0003] Deep eutectic solvents are a new type of "green solvent" composed of a mixture of two or more low-molecular-weight compounds. They possess similar physicochemical properties to ionic liquids, including low vapor pressure, low volatility, and customizable structural properties. Furthermore, they offer low raw material costs, simple preparation, and excellent biocompatibility, providing a new platform for the development of separation technologies.

[0004] Hydrophobic deep eutectic solvents have functional groups that interact with quinine, resulting in a strong capture capacity for quinine. Furthermore, these solvents possess excellent phase-forming properties, enabling phase separation at relatively low dosages. Hydroxyl-containing hydrophobic deep eutectic solvents can form hydrogen bonds with quinine, providing an effective method for extracting quinine from water. Summary of the Invention

[0005] The hydroxy hydrophobic deep eutectic solvent provided by the present invention can effectively extract quinine in water, and the method is simple to operate, highly efficient, and has a high extraction rate.

[0006] To achieve the above object, the technical solution adopted by the present invention is:

[0007] A hydroxyl hydrophobic deep eutectic solvent is used in the extraction of quinine. The method is as follows: placing the hydroxyl hydrophobic deep eutectic solvent in an aqueous solution containing quinine, and extracting quinine under constant temperature and stirring conditions; the structural formula of the hydroxyl hydrophobic deep eutectic solvent is shown in (I):

[0008]

[0009] Furthermore, in the above application method, the volume ratio of the hydroxyl hydrophobic deep eutectic solvent to the aqueous solution containing quinine is 1:80, and the concentration of quinine in the aqueous solution containing quinine is 200 mg / L.

[0010] Furthermore, in the above application method, the constant temperature is 25°C.

[0011] Furthermore, in the above application method, the stirring time is 20 minutes.

[0012] Furthermore, in the above application method, the pH value of the aqueous solution containing quinine is 10.

[0013] Furthermore, in the above application method, the preparation method of the hydroxyl hydrophobic deep eutectic solvent is as follows: menthol and thymol are mixed and stirred to react until a uniform transparent liquid is formed, and then vacuum drying is performed to remove moisture to obtain the target product.

[0014] Furthermore, in the preparation method of the above-mentioned hydroxyl hydrophobic deep eutectic solvent, the molar ratio of menthol to thymol is 1:1.

[0015] Furthermore, in the above-mentioned method for preparing the hydroxyl hydrophobic deep eutectic solvent, the temperature of the stirring reaction is 40°C.

[0016] Furthermore, in the above-mentioned method for preparing the hydroxyl hydrophobic deep eutectic solvent, the stirring reaction time is 40 minutes.

[0017] Furthermore, in the preparation method of the above-mentioned hydroxyl hydrophobic deep eutectic solvent, the vacuum drying is carried out at a vacuum degree of 0.09 MPa and a temperature of 60° C. for 48 hours.

[0018] Compared with the prior art, the present invention has the following significant advantages:

[0019] 1. The hydroxyl hydrophobic deep eutectic solvent prepared by the present invention has a wide liquid range and is non-volatile. It is an excellent green solvent and reduces energy consumption and environmental pollution.

[0020] 2. The hydroxyl hydrophobic deep eutectic solvent prepared by the present invention has a strong capture ability for quinine; at the same time, the hydrophobic deep eutectic solvent has a good phase-forming ability and can achieve phase separation at a relatively small dosage.

[0021] 3. The hydroxyl hydrophobic deep eutectic solvent prepared by the present invention can form hydrogen bonds with quinine, thereby achieving the purpose of efficiently extracting and separating quinine.

[0022] 4. The hydroxyl hydrophobic deep eutectic solvent prepared by the present invention has low price, good chemical stability, wide liquid range and high extraction rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is the hydrogen nuclear magnetic resonance spectrum of the hydroxyl hydrophobic deep eutectic solvent prepared in Example 1.

[0024] Figure 2 This is the infrared spectrum of the hydroxyl hydrophobic deep eutectic solvent prepared in Example 1.

[0025] Specific implementation methods

[0026] The present invention is further described below by way of specific examples. The present invention is not limited to the embodiments, and slight variations are possible without departing from the scope of the embodiments.

[0027] Example 1 A hydroxyl hydrophobic deep eutectic solvent for extracting quinine (I) is prepared as follows:

[0028] Menthol and thymol were weighed in a 1:1 molar ratio and placed in a sample bottle. Stirred at 40°C for 40 minutes to obtain a homogeneous, transparent liquid. Vacuum drying was then performed at 60°C for 48 hours under 0.09 MPa vacuum to obtain the final product, a menthol-thymol (1:1 molar ratio) hydrophobic deep eutectic solvent.

[0029] (2) Characterization of Hydroxy Hydrophobic Deep Eutectic Solvents:

[0030] (1) H Spectrum: H NMR Spectrum of Menthol-Thymol (Molar Ratio 1:1) Hydrophobic Deep Eutectic Solvent 1 H NMR characterization (300 MHz, solvent: DMSO-d6) Figure 1 . For H NMR spectrum analysis, 1 The chemical shift and number of resonance peaks of H NMR were consistent with those of the target hydrophobic deep eutectic solvent, and no impurity peaks were found.

[0031] (2) IR spectrum: IR characterization of menthol-thymol (molar ratio 1:1) hydrophobic deep eutectic solvent is shown in Figure 2 The infrared spectrum analysis showed that only one hydroxyl characteristic absorption peak appeared in the infrared spectrum of the deep eutectic solvent, and no new characteristic peaks were generated, indicating that there was no chemical reaction between menthol and thymol to produce new substances, but they were only combined through hydrogen bond interactions.

[0032] Example 2 Application of Hydroxyl Hydrophobic Deep Eutectic Solvent to Extract Quinine

[0033] (1) Extraction and separation experiment

[0034] Weigh an appropriate amount of quinine and dissolve it in sodium hydroxide solution at pH 10 to prepare a 200 mg / L quinine aqueous solution for later use. Pipette 0.075 mL of the hydroxyl hydrophobic deep eutectic solvent prepared in Example 1 into a 10 mL graduated test tube, followed by 6 mL of the quinine aqueous solution. The volume ratio of the hydroxyl hydrophobic deep eutectic solvent to the quinine aqueous solution is 1:80. Extraction and separation of quinine was performed at 25°C with magnetic stirring.

[0035] After 20 minutes of extraction and 2 hours of quiescence, the quinine concentration in the aqueous phase was measured at a wavelength of 330 nm using UV-visible spectrophotometry. At least three samples were taken from the aqueous phase for quantitative determination, and the relative error at equilibrium was controlled within 3% to ensure data accuracy. The quinine extraction rate E (%) was calculated according to formula (1-1):

[0036]

[0037] Where C0 is the initial concentration of quinine in water, C f is the concentration of residual quinine in the aqueous phase.

[0038] (II) Extraction rate of quinine by hydroxyl hydrophobic deep eutectic solvent

[0039] After extraction with the hydroxyl hydrophobic deep eutectic solvent, the extraction rate of quinine by the hydroxyl hydrophobic deep eutectic solvent was calculated to be 97.73%.

Claims

1. Use of a hydroxyl hydrophobic deep eutectic solvent in extracting quinine, characterized in that: The method is as follows: a hydroxyl hydrophobic deep eutectic solvent is placed in an aqueous solution containing quinine, and quinine is extracted under constant temperature and stirring conditions; the structural formula of the hydroxyl hydrophobic deep eutectic solvent is shown in (I):

2. The use of a hydroxyl hydrophobic deep eutectic solvent in extracting quinine according to claim 1, characterized in that: The volume ratio of the hydroxy hydrophobic deep eutectic solvent to the aqueous solution containing quinine is 1:80; the concentration of quinine in the aqueous solution containing quinine is 200 mg / L.

3. The use of a hydroxyl hydrophobic deep eutectic solvent in extracting quinine according to claim 1, characterized in that: The constant temperature is 25°C.

4. The use of a hydroxyl hydrophobic deep eutectic solvent in extracting quinine according to claim 1, characterized in that: The stirring time is 20 min.

5. Use of a hydroxyl hydrophobic deep eutectic solvent in extracting quinine according to claim 1, characterized in that: The pH value of the quinine-containing aqueous solution is 10.

6. Use of a hydroxyl hydrophobic deep eutectic solvent in extracting quinine according to claim 1, characterized in that: The preparation method of the hydroxyl hydrophobic deep eutectic solvent is as follows: menthol and thymol are mixed and stirred to react until a uniform transparent liquid is formed, and then vacuum drying is performed to remove moisture to obtain a target product.

7. Use of a hydroxyl hydrophobic deep eutectic solvent in extracting quinine according to claim 6, characterized in that: The molar ratio of menthol:thymol is 1:

1.

8. Use of a hydroxyl hydrophobic deep eutectic solvent in extracting quinine according to claim 6, characterized in that: The temperature of the stirring reaction was 40°C.

9. Use of a hydroxyl hydrophobic deep eutectic solvent in extracting quinine according to claim 6, characterized in that: The stirring reaction time is 40 minutes.

10. Use of a hydroxyl hydrophobic deep eutectic solvent in extracting quinine according to claim 6, characterized in that: The vacuum drying is performed at a vacuum degree of 0.09 MPa and a temperature of 60° C. for 48 hours.

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