Tissue culture device for robinia pseudoacacia propagation

Abstract

The invention relates to the technical field of plant tissue culture, in particular to a tissue culture device for robinia pseudoacacia propagation, which comprises a box body and a box door rotatably connected to the box body, the top of the box body is rotatably connected with a box cover, and the inner side of the box body is slidably connected with a movable seat. According to the robinia pseudoacacia tissue culture device, robinia pseudoacacia tissues are placed in the placement seat, then the servo motor is started to drive the lead screw to rotate reversely, so that the sealing cover moves downwards to be clamped on the placement seat, the placement seat is closed, then the fluorescent lamp is started to provide a sunlight environment for the robinia pseudoacacia tissues, and the robinia pseudoacacia tissues are cultured; the rotary knob is twisted to rotate reversely, so that the T-shaped clamping rod is separated from the groove, then the push rod is pushed to move downwards, the placing base is loosened, the sealing cover can be pulled to drive the placing base to move upwards, the robinia pseudoacacia tissue is taken out independently, the culture environment of the remaining robinia pseudoacacia tissue is not affected, and the culture effect of the robinia pseudoacacia tissue is guaranteed.

Description

Technical Field

[0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture device for Robinia pseudoacacia propagation. Background Art

[0002] Robinia pseudoacacia, a large broad-leaved tree of the genus Robinia in the Leguminosae family, is an important afforestation tree species in my country. It plays a vital role in my country's arid areas, desertification control and mine restoration. In order to obtain high-quality Robinia pseudoacacia, tissue culture is often carried out on it.

[0003] The Chinese patent with publication number CN212520330U discloses a Robinia pseudoacacia tissue culture device, which relates to the field of plant tissue culture, including a bottom plate, the upper end of the bottom plate is fixedly connected to a box body, the upper end of the box body is fixedly connected to an electric telescopic rod, the telescopic end of the electric telescopic rod passes through the box body and is fixedly connected to a hanging plate, the bottom end of the hanging plate is fixedly connected to a fluorescent lamp for providing sunlight, the four corners of the bottom end of the hanging plate are fixedly connected to the hanging rod, the hanging rod is fixedly connected to an isolation cover, the side wall of the box cavity is fixedly connected to a temperature control device for adjusting the temperature, a placement plate is movably connected to the box body, the placement plate is slidably connected to the upper end of the bottom plate, the upper end of the bottom plate is also fixedly connected to a support frame, the support frame is slidably connected to the support plate, the support plate is fixedly connected to a spray device, the box body and the isolation cover are both transparent. Although the above patent can culture Robinia pseudoacacia tissue, when a Robinia pseudoacacia tissue needs to be taken out for inspection, the isolation cover needs to be opened completely, and the Robinia pseudoacacia tissue position to be taken out cannot be opened separately for removal, resulting in the culture environment of the remaining Robinia pseudoacacia tissue being affected, affecting the culture effect of the remaining Robinia pseudoacacia tissue.

[0004] The present invention aims to solve the problems existing in the above patents. To this end, a tissue culture device for Robinia pseudoacacia reproduction is proposed, which can remove Robinia pseudoacacia tissue separately without affecting the culture environment of the remaining Robinia pseudoacacia tissue, thereby ensuring the culture effect of Robinia pseudoacacia tissue. Summary of the Invention

[0005] In order to overcome the disadvantage that although the above patent can carry out tissue culture cultivation of Robinia pseudoacacia, when a Robinia pseudoacacia tissue needs to be taken out for inspection, the isolation cover needs to be opened entirely, and the Robinia pseudoacacia tissue to be taken out cannot be opened separately for removal, resulting in the environment of the remaining Robinia pseudoacacia tissue being affected, thereby affecting the culture effect of the remaining Robinia pseudoacacia tissue, the present invention provides a tissue culture device for Robinia pseudoacacia reproduction, which can take out the Robinia pseudoacacia tissue separately without affecting the culture environment of the remaining Robinia pseudoacacia tissue, thereby ensuring the culture effect of the Robinia pseudoacacia tissue.

[0006] The present invention is achieved through the following technical approaches: a tissue culture device for the propagation of Robinia pseudoacacia, comprising a box body and a box door rotatably connected to the box body, a box cover rotatably connected to the top of the box body, a movable seat slidably connected to the inside of the box body, grooves are evenly spaced on the top of the movable seat, a placement seat for placing Robinia pseudoacacia tissue is placed in the groove of the movable seat, a movable frame is slidably connected to the inside of the box body, cavities are evenly spaced on the movable frame, a screw rod is symmetrically rotatably connected to the rear side of the box body, the screw rods on the left and right sides are driven by a synchronous belt assembly, a servo motor is installed on the outer side of the box body, the output shaft of the servo motor is fixedly connected to one of the ends of the screw rods, and also includes a sealing cover, a fluorescent lamp, a T-shaped clamping rod, a disc, a knob and a limit assembly, the sealing cover is slidably connected to the cavity of the movable frame, the sealing cover is corresponding to the placement seat, and the limit assembly is installed in the sealing The cover is used to limit the placement seat, and a fluorescent lamp is fixedly connected to the top of the sealing cover along the circumference, and slots are evenly spaced in each cavity of the movable frame. The upper part of the sealing cover is slidably connected with T-shaped clamping rods at even intervals, and the end of the T-shaped clamping rod is located in the slot. The top of the sealing cover is rotatably connected, and arc holes are evenly spaced along the circumference on the disc, and the inner side of the arc hole contacts the end of the T-shaped clamping rod. The knob is fixed to the center position of the disc, and the knob rotates through the top of the sealing cover. The knob is used to drive the disc to reverse, and the disc reversal drives the hole T-shaped clamping rod to move inward through the arc hole, and the T-shaped clamping rod is disengaged from the slot, so that the T-shaped clamping rod stops limiting the sealing cover, and then the limit assembly is pressed to stop limiting the placement seat, and the sealing cover can be pulled to drive the placement seat to move upward and take out separately to check the culture status of the locust tissue.

[0007] Further explanation: the end of the T-shaped card rod is a V-shaped sharp corner, which is used for more precise insertion into the card slot.

[0008] Further explanation, the limiting assembly includes a fixed conical disk fixed to the outer side surface of the placement seat, the fixed conical disk is an inverted cone, the shaft of the fixed conical disk is slidingly fitted with a movable conical disk, a contact plate is fixed to the movable conical disk, the left side of the contact plate is located on the inner side of the placement seat, a reset spring I is connected between the movable conical disk and the fixed conical disk, a wedge block is symmetrically slidably connected in the groove of the movable seat, the bottom of the wedge block contacts the fixed conical disk to limit the fixed conical disk, a compression spring I is connected between the wedge block and the movable seat, and a driving member is provided on the left side of the sealing cover for driving the contact plate to move downward.

[0009] To further illustrate, the side of the wedge-shaped blocks that are close to each other is an inclined surface, and the inclined surface of the wedge-shaped block is an inclined structure with a high outer surface and a low inner surface.

[0010] Further explanation, the driving member includes a push rod symmetrically slidably connected to the sealing cover, the end of the push rod is located in the placement seat and contacts the contact plate to drive the contact plate to move downward, and a support spring is connected between the push rod and the sealing cover.

[0011] Further explanation, it also includes a spray assembly, which includes a water inlet pipe fixedly connected to the movable seat, a retractable bellows on the rear side of the water inlet pipe, and guide pipes evenly spaced connected to the water inlet pipe. The guide pipes slide through the corresponding placement seat, and a hose is connected to the guide pipe for spraying nutrient solution into the placement seat. The outer side of the hose is connected to a limit rope and a reset spring III, and the reset spring III is sleeved on the limit rope. A pulling assembly is provided on the movable seat to drive the hose to swing.

[0012] Further explanation, the pulling assembly includes a contact frame that is evenly spaced and slidingly connected to the inner side of the placement seat, the end of the contact frame is in contact with the placement seat, a pull wire is symmetrically connected to the contact frame, the tail end of the pull wire is fixedly connected to the outer side of the hose, and a reset spring II is symmetrically connected between the contact frame and the movable seat.

[0013] Further description, it also includes support rods that are rotatably connected to the outer bottom of the movable seat at even intervals to support the movable seat, and a compression spring II is connected between the support rods and the movable seat.

[0014] The significant improvements of the present invention are: 1. Place the locust tissue in the placement seat, then start the servo motor to drive the screw to reverse, so that the sealing cover moves downward and is clamped on the placement seat, thereby closing the placement seat, and then start the fluorescent lamp to provide a sunlight environment for the locust tissue, thereby cultivating the locust tissue. When the locust tissue needs to be taken out separately, twist the knob to reverse, so that the T-shaped clamping rod is out of contact with the groove, and then push the push rod to move downward, so that the placement seat is released, and then pull the sealing cover to drive the placement seat to move upward so that the locust tissue is taken out separately, so as not to affect the cultivation environment of the remaining locust tissue, thereby ensuring the cultivation effect of the locust tissue.

[0015] 2. Under the action of the hose and the water inlet pipe, whenever the Robinia pseudoacacia tissue is being cultured, the nutrient solution can be discharged through the water inlet pipe, and the nutrient solution is discharged into the placement seat through the hose, which is more convenient for regular addition of the nutrient solution.

[0016] 3. Under the action of the support rod, whenever the movable seat is pulled out, the support rod swings downward and contacts the ground to support the movable seat, which can prevent the movable seat from tilting and deforming due to insufficient supporting force when being pulled out, thereby ensuring the stability of the movable seat after being pulled out. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention.

[0018] Figure 2 It is a schematic diagram of the three-dimensional structure after the present invention is unfolded.

[0019] Figure 3 It is a schematic diagram of the three-dimensional structure of the movable frame and the screw rod of the present invention.

[0020] Figure 4 It is a schematic diagram of the three-dimensional structure of the locking assembly of the present invention.

[0021] Figure 5 It is a schematic diagram of the three-dimensional structure of the limiting component of the present invention.

[0022] Figure 6 It is a schematic diagram of the three-dimensional structure of the return spring I of the present invention.

[0023] Figure 7 It is a schematic diagram of the three-dimensional structure of the liquid spraying assembly of the present invention.

[0024] Figure 8 It is a schematic diagram of the three-dimensional structure of the hose and the movable frame of the present invention.

[0025] Figure 9 It is a schematic diagram of the three-dimensional structure of the support rod of the present invention.

[0026] The markings of the various components in the accompanying drawings are as follows: 1_box body, 2_box door, 3_box cover, 4_movable seat, 5_placing seat, 6_movable frame, 7_screw, 8_servo motor, 9_sealing cover, 91_fluorescent lamp, 10_T-shaped card rod, 11_card slot, 12_disc, 13_arc hole, 14_knob, 15_fixed conical disk, 151_movable conical disk, 152_wedge block, 153_compression spring I, 154_reset spring I, 1541_contact plate, 155_push rod, 156_support spring, 16_guide pipe, 161_hose, 162_water inlet pipe, 163_contact frame, 164_reset spring II, 165_pull wire, 166_limiting rope, 167_reset spring III, 17_support rod, 18_compression spring II. DETAILED DESCRIPTION

[0027] First of all, it should be noted that in the various described embodiments, identical components are provided with identical reference numerals or identical component names, wherein the disclosure contained throughout the entire description can be transferred to the same components having the same reference numerals or identical component names. Positional designations selected in the description, such as top, bottom, lateral, etc., also refer to the directly described and illustrated figures and are transferred to the new position in the event of a change in position.

[0028] Example: A tissue culture device for Robinia pseudoacacia propagation, see Figures 1-6As shown, it includes a box body 1 and a box door 2 that is rotatably connected to the front side of the box body 1, a box cover 3 is rotatably connected to the left side of the top of the box body 1, a movable seat 4 is slidably connected to the lower inner side of the box body 1, sixteen grooves are evenly spaced on the top of the movable seat 4, and a placement seat 5 is placed in the groove of the movable seat 4. The placement seat 5 can place the Robinia pseudoacacia tissue, a movable frame 6 is slidably connected to the upper inner side of the box body 1, and sixteen cavities are evenly spaced on the movable frame 6. The back side of the box body 1 is symmetrically rotatably connected to a screw rod 7, and the screw rods on the left and right sides are symmetrically rotatable. 7 is driven by a synchronous belt assembly, and a servo motor 8 is installed on the right rear side of the outer bottom of the box body 1. The output shaft of the servo motor 8 is fixedly connected to the bottom end of the right screw rod 7. It also includes a sealing cover 9, a fluorescent lamp 91, a T-shaped card rod 10, a disc 12, a knob 14 and a limit assembly. The sealing cover 9 is slidably connected to the cavity of the movable frame 6. The sealing cover 9 corresponds to the placement seat 5. The limit assembly is installed between the sealing cover 9 and the placement seat 5. The limit assembly can limit the placement seat 5. The top of the sealing cover 9 A fluorescent lamp 91 is fixedly connected along the circumference, and four slots 11 are evenly spaced in each cavity of the movable frame 6. Four T-shaped clamping rods 10 are evenly spaced and slidably connected on the upper part of the sealing cover 9. The ends of the T-shaped clamping rods 10 are located in the slots 11. The ends of the T-shaped clamping rods 10 are V-shaped sharp corners for more accurate insertion into the slots 11. A disc 12 is rotatably connected to the top of the sealing cover 9. Arc holes 13 are evenly spaced along the circumference on the disc 12. The inner side of the arc hole 13 contacts the inner end of the T-shaped clamping rod 10. The knob 14 is fixed to the center position of the top of the disc 12, and the knob 14 rotatably passes through the top of the sealing cover 9. The knob 14 is used to drive the disc 12 to reverse. The disc 12 reverses and drives the hole T-shaped clamping rod 10 to move inward through the arc hole 13. The T-shaped clamping rod 10 is disengaged from the slot 11, so that the T-shaped clamping rod 10 stops limiting the sealing cover 9. Then, the limit assembly is pressed to stop limiting the placement seat 5. The sealing cover 9 can be pulled to drive the placement seat 5 to move upward to take out the culture status of the locust tissue separately for inspection.

[0029] See also Figure 5 and Figure 6As shown, the limiting assembly includes a fixed conical disk 15, a movable conical disk 151, a wedge block 152, a compression spring I 153, a return spring I 154, a contact plate 1541 and a driving member. The fixed conical disk 15 is fixedly connected to the middle of the outer bottom of the placement seat 5. The fixed conical disk 15 is an inverted cone. The shaft of the fixed conical disk 15 is slidably fitted with a movable conical disk 151. The left side of the movable conical disk 151 is fixedly connected to the contact plate 1541. The left side of the contact plate 1541 is located on the inner side of the placement seat 5. A return spring I 154 is connected between the bottom of the movable conical disk 151 and the top of the fixed conical disk 15. The wedge block 152 is symmetrically slidably connected in the groove of the movable seat 4. The side of the wedge block 152 close to each other is an inclined surface, and the inclined surface of the wedge block 152 is an outer high inner surface. The bottom of the inclined structure, the bottom of the wedge block 152 contacts the fixed conical disk 15, the wedge block 152 can limit the fixed conical disk 15, and the left and right wedge blocks 152 are connected to the inner side of the movable seat 4 on one side away from each other with two compression springs Ⅰ153. A driving member is provided on the left side of the sealing cover 9, and the driving member can drive the contact plate 1541 to move downward; the driving member includes a push rod 155 and a support spring 156. The left side of the sealing cover 9 is symmetrically slidably connected with the push rod 155 front and back. The bottom end of the push rod 155 is located in the placement seat 5 and contacts with the contact plate 1541. When the push rod 155 moves downward, the push rod 155 can drive the contact plate 1541 to move downward. A support spring 156 is connected between the lower part of the push rod 155 and the inner side of the sealing cover 9.

[0030] First, pull the door 2 upward to swing it open, then pull the movable seat 4 forward to move outside the box body 1, then pour an appropriate amount of culture solution into the placement seat 5, and then put the sixteen Robinia pseudoacacia tissues into the sixteen placement seats 5 respectively, so that the Robinia pseudoacacia tissues are in contact with the culture solution, and then push the movable seat 4 to reset and drive the Robinia pseudoacacia tissues to move into the box body 1, close the door 2, start the servo motor 8 to drive the screw rod 7 to reverse, the screw rod 7 reverses and drives the movable frame 6 to move downward, the movable frame 6 drives the T-shaped card rod 10 to move downward through the card slot 11, the T-shaped card rod 10 drives the sealing cover 9 to move downward through the disc 12, the sealing cover 9 drives the push rod 155 to move downward through the support spring 156, the push rod 155 moves downward to the placement seat 5 and contacts the contact plate 1541, at this time the sealing cover 9 moves downward The movable card is locked on the placement seat 5, the screw rod 7 is closed, the movable frame 6 stops moving downward, the sealing cover 9 seals the placement seat 5, and the fluorescent lamp 91 is started. The fluorescent lamp 91 provides a sunlight environment for the Robinia pseudoacacia tissue, which also cultivates the Robinia pseudoacacia tissue. When it is necessary to check the cultivation status of one of the Robinia pseudoacacia tissues, the box cover 3 is pulled upward to swing open, and then the knob 14 corresponding to the Robinia pseudoacacia tissue to be taken out is pushed to reverse, and the knob 14 drives the disc 12 to reverse, and the disc 12 drives the T-shaped card rod 10 to move inward through the arc hole 13. The T-shaped card rod 10 is disengaged from the card slot 11 and stops limiting the sealing cover 9. Then, the push rod 155 corresponding to the Robinia pseudoacacia tissue to be taken out is pushed to move downward, the support spring 156 is compressed, and the push rod 155 moves downward to drive the receiving The contact plate 1541 moves downward, and the contact plate 1541 drives the movable conical disk 151 to move downward and contact the inclined surface of the wedge block 152. The return spring I 154 is compressed, and the movable conical disk 151 drives the left and right wedge blocks 152 to move away from each other. The compression spring I 153 is compressed, and the wedge block 152 moves and disengages from the fixed conical disk 15, which also stops limiting the fixed conical disk 15. When the movable conical disk 151 continues to move downward and passes over the wedge block 152, due to the action of the compression spring I 153, the left and right wedge blocks 152 move in a direction close to each other and contact the conical surface of the movable conical disk 151, stopping the push rod 155 from moving downward and pulling the sealing cover 9 to move upward. Due to the locking of the sealing cover 9 with the placement seat 5 Then, the sealing cover 9 drives the placement seat 5 to move upward and disengage from the movable seat 4, and the placement seat 5 drives the fixed conical disk 15 to move upward. The fixed conical disk 15 drives the movable conical disk 151 to move upward and disengage from the wedge block 152 through the reset spring Ⅰ154, and the placement seat 5 continues to move upward to the outside of the box body 1 and is taken out, which makes the acacia tissue be taken out separately, and the push rod 155 is released. Due to the action of the support spring 156, the push rod 155 moves upward and resets to stop limiting the contact plate 1541. Due to the action of the reset spring Ⅰ154, the movable conical disk 151 moves upward and resets, and the sealing cover 9 can be separated from the placement seat 5 to check the cultivation status of the acacia tissue. In this way, the acacia tissue can be taken out separately to check the cultivation status.In order not to affect the culture environment of the remaining Robinia pseudoacacia tissue, thereby ensuring the culture effect of the Robinia pseudoacacia tissue, if the Robinia pseudoacacia tissue is cultivated well, the Robinia pseudoacacia tissue can be taken out from the placement seat 5, otherwise the sealing cover 9 continues to be clamped to the placement seat 5, and then the placement seat 5 is put back into the groove of the movable seat 4, and the knob 14 is twisted to rotate forward to drive the disc 12 to rotate forward. The disc 12 drives the T-shaped clamping rod 10 to move outward and insert into the groove through the arc hole 13. Since the end of the T-shaped clamping rod 10 is a V-shaped sharp angle, it can be accurately inserted into the groove. The T-shaped clamping rod 10 limits the sealing cover 9 through the groove, and then pulls the box cover 3 to swing down and close. When all the Robinia pseudoacacia tissues are cultured, the servo motor 8 is started to drive the screw 7 to rotate forward, and the screw 7 drives the movable frame 6 to move upward and reset. The movable frame 6 resets and drives the sealing cover 9 upward through the T-shaped clamping rod 10. The sealing cover 9 is separated from the placement seat 5. Then the box door 2 is pulled open, and the movable seat 4 is pulled to move the cultured Robinia pseudoacacia tissues forward through the placement seat 5 to the outside of the box body 1. The Robinia pseudoacacia tissues are then taken out of the placement seat 5 and the movable seat 4 is pushed to reset.

[0031] See also Figure 7 and Figure 8 As shown, it also includes a spray assembly installed on the movable seat 4, which includes a guide pipe 16, a hose 161, a water inlet pipe 162, a pulling assembly, a limit rope 166 and a reset spring III 167. The movable seat 4 is fixed with a water inlet pipe 162, and the rear side of the water inlet pipe 162 is a retractable bellows. Sixteen groups of guide pipes 16 are evenly spaced and connected to the water inlet pipe 162. The number of each group of guide pipes 16 is two, and each group of guide pipes 16 slides through the corresponding placement seat 5. The top of each guide pipe 16 is connected to a hose 161, which can spray the nutrient solution into the placement seat 5. The outer rear side of the hose 161 is connected to a limit rope 166 and a reset spring III 167. The return spring III 167 is sleeved on the limit rope 166, and a pulling assembly is provided on the movable seat 4. When the pulling assembly is in operation, the pulling assembly can drive the hose 161 to swing; the pulling assembly includes a contact frame 163, a return spring II 164 and a pull wire 165. Sixteen contact frames 163 are evenly spaced and slidingly connected to the inner side of the placement seat 5. The tops of the sixteen contact frames 163 are in contact with the bottoms of the sixteen placement seats 5 respectively. The lower part of the contact frame 163 is symmetrically connected with the pull wire 165. The tail ends of the pull wires 165 on the left and right sides are fixedly connected to the outer front sides of the hoses 161 on the left and right sides respectively. The return spring II 164 is symmetrically connected between the outer bottom of the contact frame 163 and the inner side of the movable seat 4.

[0032] Initially, the return spring II 164 is in a compressed state, the return spring III 167 is in a stretched state, and the water inlet pipe 162 is connected to an external water source. When the movable seat 4 drives the acacia tissue to move into the box body 1, the nutrient solution is discharged into the water inlet pipe 162, and the nutrient solution in the water inlet pipe 162 is discharged into the hose 161 through the guide pipe 16, and the hose 161 discharges the nutrient solution into the placement seat 5. The nutrient solution contacts the acacia tissue. When the placement seat 5 is filled with an appropriate amount of nutrient solution, the nutrient solution is stopped from being discharged into the water inlet pipe 162, and the acacia tissue can be added regularly. When the placement seat 5 moves upward and disengages from the movable seat 4, the placement seat 5 disengages from the contact frame 163. Due to the action of the return spring II 164, the contact frame 163 moves upward and resets, driving the pull wire 165 to move upward. The pull wire 165 is relaxed, and due to the action of the return spring III 167, the return spring III 167 drives the hose 161 to swing backward to a vertical state. The hose 161 does not affect the upward movement of the placement seat 5. When the placement seat 5 moves downward and returns to the groove of the movable seat 4, the placement seat 5 contacts the contact frame 163, which drives the contact frame 163 downward. The return spring II 164 is compressed, and the contact frame 163 drives the pull wire 165 downward. The pull wire 165 drives the hose 161 to swing forward, so that the liquid outlet end of the hose 161 faces into the placement seat 5, and the return spring III 167 is stretched. This makes it more convenient to regularly add nutrient solution.

[0033] See also Figure 9 As shown, it also includes support rods 17 and compression springs II 18. Six support rods 17 are evenly spaced and rotatably connected to the front side of the outer bottom of the movable seat 4. When the support rods 17 swing downward, the support rods 17 can support the movable seat 4. A compression spring II 18 is connected between the top of the support rods 17 and the inner side of the movable seat 4.

[0034] Initially, compression spring II 18 is in a compressed state. When the movable seat 4 drives the placement seat 5 forward, the movable seat 4 drives the support rod 17 forward, and the support rod 17 loses contact with the box body 1. Due to the action of compression spring II 18, the support rod 17 swings downward and contacts the ground. The support rod 17 supports the movable seat 4, and the placement and removal of the Robinia pseudoacacia tissue can be completed. When the movable seat 4 moves backward and resets, the movable seat 4 drives the support rod 17 backward and resets. The support rod 17 moves backward and contacts the box body 1. The box body 1 drives the support rod 17 to swing upward and reset, and the compression spring II 18 is compressed. In this way, the movable seat 4 can be prevented from tilting and deforming due to insufficient support force when it is pulled out, thereby ensuring the stability of the movable seat 4 after it is pulled out.

[0035] Finally, it is necessary to point out that the above content is only used to help understand the technical solution of the present invention and cannot be understood as limiting the scope of protection of the present invention; non-essential improvements and adjustments made by technical personnel in this field based on the above content of the present invention are all within the scope of protection required by the present invention.

Claims

1. A tissue culture device for propagation of Robinia pseudoacacia, comprising a box body (1) and a box door (2) rotatably connected to the box body (1), a box cover (3) rotatably connected to the top of the box body (1), a movable seat (4) slidably connected to the inside of the box body (1), grooves are evenly spaced on the top of the movable seat (4), and a placement seat (5) for placing Robinia pseudoacacia tissue is placed in the groove of the movable seat (4), a movable frame (6) is slidably connected to the inside of the box body (1), cavities are evenly spaced on the movable frame (6), a screw rod (7) is symmetrically rotatably connected to the rear side of the box body (1), the screw rods (7) on the left and right sides are driven by a synchronous belt assembly, a servo motor (8) is installed on the outer side of the box body (1), and the output shaft of the servo motor (8) is fixedly connected to the end of one of the screw rods (7), wherein the servo motor (8) is fixedly connected to the end of one of the screw rods (7), and the servo motor (8) is characterized in that: The invention also includes a sealing cover (9), a fluorescent lamp (91), a T-shaped clamping rod (10), a disc (12), a knob (14) and a limit assembly, wherein the sealing cover (9) is slidably connected to the cavity of the movable frame (6), the sealing cover (9) corresponds to the placement seat (5), the limit assembly is installed between the sealing cover (9) and the placement seat (5), and is used to limit the placement seat (5), the top of the sealing cover (9) is fixedly connected with the fluorescent lamp (91) along the circumferential direction, each cavity of the movable frame (6) is provided with a clamping groove (11) at uniform intervals, the upper part of the sealing cover (9) is slidably connected with a T-shaped clamping rod (10), the end of the T-shaped clamping rod (10) is located in the clamping groove (11), the top of the sealing cover (9) is rotatably connected with the disc (12), the disc (1 2) The upper edge is provided with arc-shaped holes (13) spaced evenly in the circumferential direction, the inner side of the arc-shaped holes (13) contacts the end of the T-shaped clamping rod (10), the knob (14) is fixed to the center of the disc (12), the knob (14) rotatably penetrates the top of the sealing cover (9), the knob (14) is used to drive the disc (12) to reverse, the disc (12) reverses and drives the hole T-shaped clamping rod (10) to move inward through the arc-shaped hole (13), the T-shaped clamping rod (10) is disengaged from the slot (11), so that the T-shaped clamping rod (10) stops limiting the sealing cover (9), and then the limiting component is pressed to stop limiting the placement seat (5), and the sealing cover (9) can be pulled to drive the placement seat (5) to move upward to take out the culture status of the locust tissue separately for inspection.

2. A tissue culture device for Robinia pseudoacacia propagation according to claim 1, characterized in that: The end of the T-shaped card rod (10) is a V-shaped sharp corner, which is used for more accurate insertion into the card slot (11).

3. A tissue culture device for Robinia pseudoacacia propagation according to claim 2, characterized in that: The limiting assembly includes a fixed conical disk (15) fixed to the outer side of the placement seat (5), the fixed conical disk (15) is an inverted cone, the shaft of the fixed conical disk (15) is slidably covered with a movable conical disk (151), a contact plate (1541) is fixed to the movable conical disk (151), the left side of the contact plate (1541) is located on the inner side of the placement seat (5), a return spring I (154) is connected between the movable conical disk (151) and the fixed conical disk (15), a wedge block (152) is symmetrically slidably connected in the groove of the movable seat (4), the bottom of the wedge block (152) contacts the fixed conical disk (15) to limit the fixed conical disk (15), a compression spring I (153) is connected between the wedge block (152) and the movable seat (4), and a driving member is provided on the left side of the sealing cover (9) for driving the contact plate (1541) to move downward.

4. A tissue culture device for Robinia pseudoacacia propagation according to claim 3, characterized in that: The sides of the wedge-shaped blocks (152) that are close to each other are inclined surfaces, and the inclined surfaces of the wedge-shaped blocks (152) are inclined structures with a high outer surface and a low inner surface.

5. A tissue culture device for Robinia pseudoacacia propagation according to claim 4, characterized in that: The driving member includes a push rod (155) symmetrically slidably connected to the sealing cover (9), the end of the push rod (155) is located in the placement seat (5) and contacts the contact plate (1541) to drive the contact plate (1541) to move downward, and a support spring (156) is connected between the push rod (155) and the sealing cover (9).

6. The tissue culture device for Robinia pseudoacacia propagation according to claim 5, characterized in that: The invention also includes a liquid spraying assembly, which includes a water inlet pipe (162) fixedly connected to the movable seat (4), the rear side of the water inlet pipe (162) is a retractable bellows, and the water inlet pipe (162) is evenly spaced and connected with a guide pipe (16), which slides through the corresponding placement seat (5), and is connected with a hose (161) for spraying the nutrient solution into the placement seat (5). The outer side of the hose (161) is connected to a limit rope (166) and a return spring III (167), and the return spring III (167) is sleeved on the limit rope (166). A pulling assembly is provided on the movable seat (4) for driving the hose (161) to swing.

7. The tissue culture device for Robinia pseudoacacia propagation according to claim 6, characterized in that: The pulling assembly includes a contact frame (163) slidably connected to the inner side of the placement seat (5) at uniform intervals, the end of the contact frame (163) contacts the placement seat (5), a pull wire (165) is symmetrically connected to the contact frame (163), the tail end of the pull wire (165) is fixedly connected to the outer side of the hose (161), and a return spring II (164) is symmetrically connected between the contact frame (163) and the movable seat (4).

8. The tissue culture device for Robinia pseudoacacia propagation according to claim 7, characterized in that: It also includes support rods (17) that are rotatably connected to the outer bottom of the movable seat (4) at even intervals and are used to support the movable seat (4). A compression spring II (18) is connected between the support rods (17) and the movable seat (4).

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