Artificial breeding method of hybrid salamander

Abstract

The application provides an artificial breeding method of Pseudobagrus vachelli hybrid, and relates to the field of aquaculture.The artificial breeding method of Pseudobagrus vachelli hybrid comprises the following steps: (1) parent selection; (2) parent cultivation: feeding female parent fish feed I and male parent fish feed II three months before breeding; feed I raw materials: fish meal, soybean meal, corn meal, astaxanthin, beta-glucan, compound microbial agent, compound A; feed II raw materials: fish meal, squid meal, saltwort powder, astaxanthin, rosemary acid, compound microbial agent, compound B; compound microbial agent raw materials: Bacillus ligniniphilus, Bacillus pumilus and Bacillus natto; compound A raw materials: Dendrobium officinale, Gynostemma pentaphyllum, ramie root, Aralia decaisneana leaf and perilla seed; compound B raw materials: sea horse, Cistanche, Acanthopanax, water caltrop shell and moringa seed; (3) fertilization and hatching to obtain fry.The application has the advantages of high egg-laying amount of Pseudobagrus vachelli hybrid single-tail female fish, high fertilization rate and high hatching rate, and reduces fry deformity.

Description

Technical Field

[0001] This invention relates to the field of aquaculture, and in particular to a method for the artificial breeding of hybrid spotted stag beetle. Background Technology

[0002] Clearwater grouper (Shu-zukuri) Epinephelus Polyphekadion It has delicious flesh and is highly resilient. Brown-spotted grouper (tiger grouper) Epinephelus Fuscoguttatus It has a wide temperature tolerance, fast growth rate, delicious meat, and high nutritional value. The Ficus Tiger Hybrid Grouper is a hybrid variety bred by using the freshwater grouper as the female parent and the brown-spotted grouper as the male parent.

[0003] Traditional methods of raising hybrid grouper (Gymnocypris chinensis) involve long rearing cycles, susceptibility to disease, and reduced survival rates in adult fish. Artificial breeding of superior strains is an effective way to address these problems at their source. Traditional methods for raising hybrid grouper fry result in limited egg production, low fertilization and hatching rates, and some fry are deformed; outdoor rearing is even more challenging. Therefore, there is an urgent need to develop an artificial breeding method for hybrid grouper. Summary of the Invention

[0004] In view of this, the present invention proposes an artificial breeding method for hybrid spotted sedge to solve the above problems.

[0005] The technical solution of this invention is achieved as follows: an artificial breeding method for hybrid spotted spruce tiger, comprising the following steps:

[0006] (1) Selection of parent fish: Select healthy freshwater grouper aged 3-4 years as female parent fish; select healthy brown-spotted grouper aged 1-2 years as male parent fish;

[0007] (2) Parent rearing: Three months before breeding, female parent fish are fed with fortified feed I and male parent fish are fed with fortified feed II. Feed I includes the following ingredients: fish meal, soybean meal, corn meal, astaxanthin, β-glucan, compound microbial agent, and compound A. Feed II includes the following ingredients: fish meal, squid meal, Dunaliella salina powder, astaxanthin, rosmarinic acid, compound microbial agent, and compound B. Compound microbial agent includes the following ingredients: Acetobacter xylinum, Bacillus pumilus, and Bacillus natto. Compound A includes the following ingredients: Dendrobium officinale, Gynostemma pentaphyllum, ramie root, Aralia elata leaves, and Perilla frutescens seeds. Compound B includes the following ingredients: seahorse, Cistanche deserticola, Acanthopanax senticosus, water chestnut shell, and Moringa seeds.

[0008] (3) Fertilization and hatching: When the parent fish reach sexual maturity, they will naturally lay eggs and fertilize them. The fertilized eggs will be transferred to an incubator for hatching to obtain fry.

[0009] Furthermore, the parent stock cultivation environment is as follows: open-air elevated ponds with a water depth of 2-4 meters are selected for cultivation, with a cultivation density of 3-5 fish / m³. 3 .

[0010] Furthermore, the feed I comprises the following raw materials in parts by weight: 25-35 parts fish meal, 15-25 parts soybean meal, 10-20 parts corn meal, 3-8 parts astaxanthin, 8-12 parts β-glucan, 2-4 parts compound microbial agent, and 15-20 parts compound A; the daily feeding amount of the feed I is 2%-3% of the parent's weight, and it is fed 2-3 times a day.

[0011] Furthermore, the feed II comprises the following raw materials in parts by weight: 20-30 parts fish meal, 20-30 parts squid meal, 10-20 parts Dunaliella salina powder, 8-12 parts astaxanthin, 3-8 parts rosmarinic acid, 3-8 parts compound microbial agent, and 13-18 parts compound B; the daily feeding amount of the feed II is 1%-2% of the parent's weight, and it is fed 2-3 times a day.

[0012] Furthermore, the complex A comprises the following raw materials in parts by weight: 25-35 parts of Dendrobium officinale, 20-30 parts of Gynostemma pentaphyllum, 15-25 parts of Ramie root, 10-20 parts of Aralia elata leaves, and 8-12 parts of Perilla frutescens seeds.

[0013] Furthermore, the complex B comprises the following raw materials in parts by weight: 20-30 parts seahorse, 20-30 parts Cistanche deserticola, 15-25 parts Acanthopanax senticosus, 10-20 parts water chestnut shell, and 10-20 parts Moringa seeds.

[0014] Furthermore, the mass ratio of Acetobacter xylinum, Bacillus pumilus, and Bacillus natto in the compound microbial agent is 4-6:3-4:1-2.

[0015] Furthermore, during the parent breeding process, 24 hours before a heavy rain, an additional fortifying agent is applied at a rate of 0.4-0.6 g / m². 2 The reinforcing agent comprises the following raw materials in parts by weight: 25-35 parts of Aralia elata extract, 4-6 parts of L-valine, 8-12 parts of thiamine, 10-20 parts of disodium ethylenediaminetetraacetate, 15-25 parts of magnesium sulfate, and 15-25 parts of calcium dihydrogen phosphate.

[0016] Furthermore, before adding fertilized eggs, the incubator contains seawater, pullulan is added, and the mixture is plasma-treated at 60-70W and 40-50kHz for 2-4 minutes. The amount of pullulan added is 0.05%-0.1% of the seawater.

[0017] Furthermore, after adding the fertilized egg, it is magnetized at 50-80 Gs for 20-30 min.

[0018] Furthermore, during the incubation process, the water temperature is 22-28℃, the salinity is 28‰-30‰, the pH value is 7.5-8.5, the oxygen concentration is 4-6mg / L, and the fertilized egg density is 30,000-40,000 eggs / m³. 3 .

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] (1) The artificial breeding method of the hybrid grouper of the Chinese jujube and tiger jujube of the present invention results in a high egg production rate for single female fish, as well as a high fertilization and hatching rate, minimizing malformation in fry. Specifically, the present invention uses a complex A prepared from ramie root, Aralia elata leaves, Dendrobium officinale, Gynostemma pentaphyllum, and Perilla frutescens seeds, which promotes the maturation of the female fish's gonads, increases the number and quality of eggs produced, improves fertilization and hatching rates, and reduces the malformation rate. The present invention uses a complex B prepared from Acanthopanax senticosus, water chestnut shell, seahorse, Cistanche deserticola, and Moringa seeds, which promotes the maturation of the male fish's gonads, improves sperm quality, significantly increases fertilization and hatching rates, and significantly reduces the malformation rate. The present invention uses Acetobacter xylinum, Bacillus pumilus, and Bacillus natto to prepare feed I and feed II, which further promotes the gonadal development of both male and female fish and improves the quality of fertilized eggs. Simultaneously, feed I and feed II prepared by the present invention also effectively improve the immunity and stress resistance of the parent fish, enhancing their reproductive capacity.

[0021] (2) The present invention introduces a fortifier prepared by combining gentian root extract with L-valine, thiamine, disodium ethylenediaminetetraacetate, magnesium sulfate and calcium dihydrogen phosphate before heavy rain during the parent breeding process, which helps to reduce the adverse effects of heavy rain on the gonadal development of the parent.

[0022] (3) The present invention selects an open-air high-level pond for parent stock cultivation. Combining the method of the present invention can not only overcome the adverse effects of weather, but also improve the stress resistance of the parent stock and enhance its breeding quality. Detailed Implementation

[0023] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0024] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0025] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0026] The Acetobacter xylinum, Bacillus pumilus, and Bacillus natto used in the embodiments of this invention are all commercially available strains.

[0027] Example 1

[0028] A method for artificially breeding hybrid spotted sable stags includes the following steps:

[0029] (1) Parent selection: Select healthy freshwater grouper aged 3-4 years as female parents; select healthy brown-spotted grouper aged 1-2 years as male parents.

[0030] (2) Parent breeding:

[0031] Rearing environment: Select an open-air elevated pond with a water depth of 2-4m for rearing, with a rearing density of 3-5 fish / m³. 3 ;

[0032] Three months before breeding, female broodstock should be fed fortified feed I, and male broodstock should be fed fortified feed II. Feed I consists of the following ingredients: 30 parts fish meal, 20 parts soybean meal, 15 parts corn meal, 5 parts astaxanthin, 10 parts β-glucan, 3 parts compound microbial agent, and 17 parts compound A. The daily feeding amount of feed I is 2%-3% of the weight of the female broodstock, and it should be fed 2-3 times a day.

[0033] Feed II comprises the following ingredients by weight: 25 parts fish meal, 25 parts squid meal, 15 parts Dunaliella salina powder, 10 parts astaxanthin, 5 parts rosmarinic acid, 5 parts compound microbial agent, and 15 parts compound B. The daily feeding amount of Feed II is 1%-2% of the weight of the male parent fish, and it is fed 2-3 times a day.

[0034] The compound microbial agent consists of Acetobacter xylinum, Bacillus pumilus, and Bacillus natto in a mass ratio of 5:3.5:1.5.

[0035] Complex A comprises the following raw materials in parts by weight: 30 parts Dendrobium officinale, 25 parts Gynostemma pentaphyllum, 20 parts Ramie root, 15 parts Aralia elata leaves, and 10 parts Perilla frutescens seeds;

[0036] Complex B comprises the following raw materials in parts by weight: 25 parts seahorse, 25 parts Cistanche deserticola, 20 parts Acanthopanax senticosus, 15 parts water chestnut shell, and 15 parts Moringa seeds;

[0037] During the parent breeding process, 24 hours before the heavy rain, an additional fortifying agent was applied at a rate of 0.5 g / m². 2 The reinforcing agent comprises the following raw materials in parts by weight: 30 parts of Aralia elata extract, 5 parts of L-valine, 10 parts of thiamine, 15 parts of disodium EDTA, 20 parts of magnesium sulfate, and 20 parts of calcium dihydrogen phosphate.

[0038] (3) Fertilization and Hatching: Once the parent fish reach sexual maturity, they will naturally spawn and fertilize. The fertilized eggs will be transferred to an incubation tank (containing seawater) for hatching. During the hatching process, the water temperature will be controlled at 22-28℃, salinity at 28‰-30‰, pH at 7.5-8.5, and oxygen concentration at 4-6 mg / L. Before adding the fertilized eggs, add 0.08% pullulan polysaccharide by weight of seawater and treat with plasma at 65W and 45kHz for 3 minutes. Add the fertilized eggs (fertilized egg density 30,000-40,000 eggs / m³). 3 After that, the fish were magnetized at 65 Gs for 25 min, and then hatched to obtain fry.

[0039] Example 2

[0040] A method for artificially breeding hybrid spotted sable stags includes the following steps:

[0041] (1) Parent selection: Select healthy freshwater grouper aged 3-4 years as female parents; select healthy brown-spotted grouper aged 1-2 years as male parents.

[0042] (2) Parent breeding:

[0043] Rearing environment: Select an open-air elevated pond with a water depth of 2-4m for rearing, with a rearing density of 3-5 fish / m³. 3 ;

[0044] Three months before breeding, female broodstock should be fed fortified feed I, and male broodstock should be fed fortified feed II. Feed I consists of the following ingredients: 25 parts fish meal, 22 parts soybean meal, 20 parts corn meal, 3 parts astaxanthin, 8 parts β-glucan, 2 parts compound microbial agent, and 20 parts compound A. The daily feeding amount of feed I is 2%-3% of the broodstock weight, and it should be fed 2-3 times a day.

[0045] Feed II comprises the following ingredients by weight: 20 parts fish meal, 30 parts squid meal, 10 parts Dunaliella salina powder, 12 parts astaxanthin, 5 parts rosmarinic acid, 5 parts compound microbial agent, and 15 parts compound B. The daily feeding amount of Feed II is 1%-2% of the parent's weight, and it is fed 2-3 times a day.

[0046] The compound microbial agent consists of Acetobacter xylinum, Bacillus pumilus, and Bacillus natto in a mass ratio of 6:3:1.

[0047] Complex A comprises the following raw materials in parts by weight: 27 parts Dendrobium officinale, 30 parts Gynostemma pentaphyllum, 15 parts Ramie root, 20 parts Aralia elata leaves, and 8 parts Perilla frutescens seeds;

[0048] Complex B comprises the following raw materials in parts by weight: 23 parts seahorse, 30 parts Cistanche deserticola, 17 parts Acanthopanax senticosus, 10 parts water chestnut shell, and 20 parts Moringa seeds;

[0049] During the parent breeding process, 24 hours before the heavy rain, an additional fortifying agent was applied at a rate of 0.5 g / m². 2 The reinforcing agent comprises the following raw materials in parts by weight: 28 parts of Aralia elata extract, 4 parts of L-valine, 8 parts of thiamine, 20 parts of disodium EDTA, 15 parts of magnesium sulfate, and 25 parts of calcium dihydrogen phosphate.

[0050] (3) Fertilization and Hatching: Once the parent fish reach sexual maturity, they will naturally spawn and fertilize. The fertilized eggs will be transferred to an incubation tank (containing seawater) for hatching. During the hatching process, the water temperature will be controlled at 22-28℃, salinity at 28‰-30‰, pH at 7.5-8.5, and oxygen concentration at 4-6 mg / L. Before adding the fertilized eggs, add 0.05% pullulan polysaccharide by weight of seawater and treat with plasma at 60W and 40kHz for 4 minutes. Add the fertilized eggs (fertilized egg density 30,000-40,000 eggs / m³). 3 After that, the fish were magnetized at 50 Gs for 30 min, and then hatched to obtain fry.

[0051] Example 3

[0052] A method for artificially breeding hybrid spotted sable stags includes the following steps:

[0053] (1) Parent selection: Select healthy freshwater grouper aged 3-4 years as female parents; select healthy brown-spotted grouper aged 1-2 years as male parents.

[0054] (2) Parent breeding:

[0055] Rearing environment: Select an open-air elevated pond with a water depth of 2-4m for rearing, with a rearing density of 3-5 fish / m³. 3 ;

[0056] Three months before breeding, female broodstock should be fed with fortified feed I, and male broodstock should be fed with fortified feed II. Feed I consists of the following ingredients: 35 parts fish meal, 15 parts soybean meal, 10 parts corn meal, 8 parts astaxanthin, 12 parts β-glucan, 4 parts compound microbial agent, and 16 parts compound A. The daily feeding amount of feed I is 2%-3% of the weight of the broodstock, and it should be fed 2-3 times a day.

[0057] Feed II comprises the following ingredients by weight: 30 parts fish meal, 20 parts squid meal, 18 parts Dunaliella salina powder, 8 parts astaxanthin, 3 parts rosmarinic acid, 8 parts compound microbial agent, and 13 parts compound B. The daily feeding amount of Feed II is 1%-2% of the parent's weight, and it is fed 2-3 times a day.

[0058] The compound microbial agent consists of Acetobacter xylinum, Bacillus pumilus, and Bacillus natto in a mass ratio of 4:4:2.

[0059] Complex A comprises the following raw materials in parts by weight: 33 parts Dendrobium officinale, 20 parts Gynostemma pentaphyllum, 25 parts Ramie root, 10 parts Aralia elata leaves, and 12 parts Perilla frutescens seeds;

[0060] Complex B comprises the following raw materials in parts by weight: 28 parts seahorse, 20 parts Cistanche deserticola, 22 parts Acanthopanax senticosus, 20 parts water chestnut shell, and 10 parts Moringa seeds;

[0061] During the parent breeding process, 24 hours before the heavy rain, an additional fortifying agent was applied at a rate of 0.5 g / m². 2 The reinforcing agent comprises the following raw materials in parts by weight: 32 parts of Aralia elata extract, 6 parts of L-valine, 12 parts of thiamine, 10 parts of disodium EDTA, 25 parts of magnesium sulfate, and 15 parts of calcium dihydrogen phosphate.

[0062] (3) Fertilization and Hatching: Once the parent fish reach sexual maturity, they will naturally spawn and fertilize. The fertilized eggs will be transferred to an incubation tank (containing seawater) for hatching. During the hatching process, the water temperature will be controlled at 22-28℃, the salinity at 28‰-30‰, the pH at 7.5-8.5, and the oxygen concentration at 4-6 mg / L. Before adding the fertilized eggs, add 0.1% pullulan polysaccharide by weight of the seawater and treat with plasma at 70W and 50kHz for 2 minutes. Add the fertilized eggs (fertilized egg density 30,000-40,000 eggs / m³). 3 After that, the fish were magnetized at 80 Gs for 20 minutes, and then hatched to obtain fry.

[0063] Comparative Example 1

[0064] The main difference from Example 1 is that Complex A does not contain ramie root or Aralia elata leaves, but increases the amount of Dendrobium officinale and Gynostemma pentaphyllum by the same amount. Complex A comprises the following raw materials in parts by weight: 50 parts Dendrobium officinale, 40 parts Gynostemma pentaphyllum, and 10 parts Perilla frutescens seeds.

[0065] Comparative Example 2

[0066] The main difference from Example 1 is that Complex B does not contain Acanthopanax senticosus or water chestnut shells, but increases the amount of seahorse and Cistanche deserticola by the same amount. Complex B comprises the following raw materials in parts by weight: 45 parts seahorse, 40 parts Cistanche deserticola, and 15 parts Moringa seeds;

[0067] Comparative Example 3

[0068] The main difference from Example 1 is that Acetobacter xylinum in the compound microbial agent is replaced with Lactobacillus rhamnosus, wherein the compound microbial agent is composed of Lactobacillus rhamnosus, Bacillus pumilus and Bacillus natto in a mass ratio of 5:3.5:1.5;

[0069] Comparative Example 4

[0070] The main difference from Example 1 is that Aralia elata extract is replaced with Gentiana scabra root extract.

[0071] During the parent breeding process, 24 hours before the heavy rain, an additional fortifying agent was applied at a rate of 0.5 g / m². 2 The reinforcing agent comprises the following raw materials in parts by weight: 30 parts gentian root extract, 5 parts L-valine, 10 parts thiamine, 15 parts disodium ethylenediaminetetraacetate, 20 parts magnesium sulfate, and 20 parts calcium dihydrogen phosphate.

[0072] Test case

[0073] The breeding methods of Examples 1-3 and Comparative Examples 1-4 were used for the experiment. 200 female parent fish were used in each group. The number of eggs laid, fertilization rate, hatching rate, and larval deformity rate were recorded and the average values ​​were calculated, as shown in Table 1 below.

[0074] Table 1 - Artificial Breeding of the Chinese Fir-tailed Tiger Hybrid

[0075]

[0076] As shown in the table above, the single female fish in Example 1 had the highest egg production, fertilization rate, and hatching rate, and no larval deformities were observed. Examples 2-3 also had high egg production per single female fish, along with high fertilization and hatching rates, and low larval deformity rates.

[0077] Compared to Example 1, Comparative Example 1's Compound A did not contain ramie root or Aralia elata leaves, but the amounts of Dendrobium officinale and Gynostemma pentaphyllum were increased by the same amount. Comparative Example 1 showed a significant decrease in egg production, fertilization rate, and hatching rate, and an increase in the rate of deformed larvae. The present invention uses Compound A prepared from ramie root, Aralia elata leaves, Dendrobium officinale, Gynostemma pentaphyllum, and Perilla frutescens seeds, which is beneficial for the gonadal development and maturation of female fish, increasing both the quantity and quality of eggs laid, improving fertilization and hatching rates, and reducing the rate of deformities.

[0078] In Comparative Example 2, Complex B did not contain Acanthopanax senticosus or water chestnut shells, but the amounts of seahorse and Cistanche deserticola were increased by the same amount. Comparative Example 2 showed a significant decrease in spawning, fertilization rate, and hatching rate, and a significant increase in the larval deformity rate. This invention uses Acanthopanax senticosus and water chestnut shells, combined with seahorse, Cistanche deserticola, and Moringa seeds to prepare Complex B, which is beneficial for the gonadal development and maturation of male fish, improves sperm quality, significantly increases fertilization and hatching rates, and significantly reduces the deformity rate.

[0079] In Comparative Example 3, replacing *Acetobacter xylinum* with *Lactobacillus rhamnosus* in the compound microbial agent resulted in feeds I and II that negatively impacted gonadal development in both male and female Amur bream, affecting fertilization efficiency, leading to decreased egg quality, reduced hatching rate, and increased larval deformity rate. This invention, using *Acetobacter xylinum*, *Bacillus pumilus*, and *Bacillus natto* to prepare feeds I and II, is more beneficial for improving gonadal development in both male and female fish and enhancing egg quality.

[0080] In Comparative Example 4, replacing Aralia elata extract with Gentiana scabra root extract significantly reduced the breeding effect. The enhancer in this invention uses Gentiana scabra root extract combined with L-valine, thiamine, disodium EDTA, magnesium sulfate, and calcium dihydrogen phosphate, which helps reduce the adverse effects of heavy rain on the parent plants.

[0081] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the scope of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for artificially breeding hybrid spotted sedge, characterized in that, Includes the following steps: (1) Selection of parent fish: Select healthy freshwater grouper aged 3-4 years as female parent fish; select healthy brown-spotted grouper aged 1-2 years as male parent fish; (2) Parent rearing: Three months before breeding, female parent fish are fed with fortified feed I and male parent fish are fed with fortified feed II. Feed I includes the following ingredients: fish meal, soybean meal, corn meal, astaxanthin, β-glucan, compound microbial agent, and compound A. Feed II includes the following ingredients: fish meal, squid meal, Dunaliella salina powder, astaxanthin, rosmarinic acid, compound microbial agent, and compound B. Compound microbial agent includes the following ingredients: Acetobacter xylinum, Bacillus pumilus, and Bacillus natto. Compound A includes the following ingredients: Dendrobium officinale, Gynostemma pentaphyllum, ramie root, Aralia elata leaves, and Perilla frutescens seeds. Compound B includes the following ingredients: seahorse, Cistanche deserticola, Acanthopanax senticosus, water chestnut shell, and Moringa seeds. (3) Fertilization and hatching: When the parent fish reach sexual maturity, they will naturally lay eggs and fertilize them. The fertilized eggs will be transferred to an incubator for hatching to obtain fry.

2. The method for artificially breeding the hybrid spotted spruce-tiger according to claim 1, characterized in that, The parent stock were raised in an open-air, elevated pond with a water depth of 2-4 meters, at a stocking density of 3-5 fish / m³. 3 .

3. The method for artificially breeding the hybrid spotted spruce-tiger according to claim 1, characterized in that, The feed I comprises the following ingredients in parts by weight: 25-35 parts fish meal, 15-25 parts soybean meal, 10-20 parts corn meal, 3-8 parts astaxanthin, 8-12 parts β-glucan, 2-4 parts compound microbial agent, and 15-20 parts compound A. The daily feeding amount of the feed I is 2%-3% of the parent's weight, and it is fed 2-3 times a day.

4. The method for artificially breeding the hybrid spotted spruce-tiger according to claim 1, characterized in that, The feed II comprises the following ingredients in parts by weight: 20-30 parts fish meal, 20-30 parts squid meal, 10-20 parts Dunaliella salina powder, 8-12 parts astaxanthin, 3-8 parts rosmarinic acid, 3-8 parts compound microbial agent, and 13-18 parts compound B. The daily feeding amount of the feed II is 1%-2% of the parent's weight, and it is fed 2-3 times a day.

5. The method for artificially breeding the hybrid spotted spruce tiger according to claim 1 or 3, characterized in that, The complex A comprises the following raw materials in parts by weight: 25-35 parts of Dendrobium officinale, 20-30 parts of Gynostemma pentaphyllum, 15-25 parts of ramie root, 10-20 parts of Aralia elata leaf, and 8-12 parts of Perilla frutescens seed.

6. The method for artificially breeding the hybrid spotted spruce-tiger according to claim 1 or 4, characterized in that, The complex B comprises the following raw materials in parts by weight: 20-30 parts seahorse, 20-30 parts Cistanche deserticola, 15-25 parts Acanthopanax senticosus, 10-20 parts water chestnut shell, and 10-20 parts Moringa seeds.

7. The method for artificially breeding the hybrid spotted spruce-tiger according to claim 1, characterized in that, The mass ratio of Acetobacter xylinum, Bacillus pumilus, and Bacillus natto in the compound microbial agent is 4-6:3-4:1-2.

8. The method for artificially breeding the jujube-tiger hybrid spotted jujube according to claim 1, characterized in that, During the parent breeding process, 24 hours before a heavy rain, an additional fortifying agent was applied at a rate of 0.4-0.6 g / m³. 2 The reinforcing agent comprises the following raw materials in parts by weight: 25-35 parts of Aralia elata extract, 4-6 parts of L-valine, 8-12 parts of thiamine, 10-20 parts of disodium ethylenediaminetetraacetate, 15-25 parts of magnesium sulfate, and 15-25 parts of calcium dihydrogen phosphate.

9. The method for artificially breeding the hybrid spotted spruce-tiger according to claim 1, characterized in that, Before adding fertilized eggs, the incubator contains seawater and pullulan. The mixture is then subjected to plasma treatment at 60-70W and 40-50kHz for 2-4 minutes. The amount of pullulan added is 0.05%-0.1% of the seawater.

10. The method for artificially breeding the hybrid spotted spruce tiger according to claim 1 or 9, characterized in that, After adding the fertilized egg, magnetize it at 50-80 Gs for 20-30 min.

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