Method for shortening artificial breeding cycle of mandarin fish

Abstract

The invention discloses a method for shortening the artificial breeding period of mandarin fish, and belongs to the technical field of fish fry breeding. Comprising the following steps that a siniperca chuatsi parent fish rearing and breeding system which is free of natural light irradiation and provided with a full-spectrum irradiation lamp, a water temperature control system and a circulating water treatment system is constructed; selecting sexually mature siniperca chuatsi parent fish; in a parent fish rearing system without natural light irradiation, the environment condition of low-temperature natural overwintering of siniperca chuatsi is simulated, and the water temperature and the irradiation period of an irradiation lamp are set; selecting mature parent fish individuals according to the behavior characteristics and the morphological characteristics; and artificial spawning induction and insemination. According to the method, the siniperca chuatsi parent fish is bred through the circulating water culture system, temperature and illumination are regulated and controlled through matched automatic temperature control equipment and intelligent light environment regulation and control equipment, gonad development of the parent fish can be effectively promoted, the spawning time of the parent fish can be precisely regulated and controlled, and technical support is provided for stable production of siniperca chuatsi fry.

Description

Technical Field

[0001] The present invention relates to a method for shortening the artificial reproduction cycle of mandarin fish, in particular to a method for promoting gonadal development of parent fish by artificially simulating the natural overwintering conditions of mandarin fish at low temperatures and feeding with hormones under factory-scale recirculating aquaculture conditions. The present invention belongs to the technical field of fish seed breeding, and specifically relates to a method for shortening the artificial reproduction cycle of mandarin fish. Background Art

[0002] Seedlings are the material foundation for the sustainable development of aquaculture, and broodstock cultivation is a key step in improving seed quality. Broodstock cultivation and maturation technologies are crucial for ensuring a stable supply of seedlings. Fish have an inherent reproductive rhythm, and spawning timing is influenced by factors such as temperature, light, and hormones. The initiation of the broodstock gonadal maturation cycle is related to seasonal fluctuations in temperature, light, and hormones.

[0003] The mandarin fish (Siniperca c huatsi), also known as the mandarin fish and sweet-scented fish, is the fastest-growing and largest of the nine known species of fish in the subfamily Sinipercainae of the order Perciformes, and is therefore of great economic value. Under natural conditions, the mandarin fish's breeding season runs from April to September, with a 150-180-day culture cycle. The fish are primarily available from October to February. However, a shortage of late-season mandarin fish seedlings leads to a surge in prices between April and August of the following year. To ensure a stable supply of high-quality late-season mandarin fish seedlings, effective methods are needed to cultivate and mature the broodstock, thereby promoting the healthy, stable, and sustainable development of the mandarin fish aquaculture industry. Currently, the artificial breeding cycle for mandarin fish ends in September. To shorten the gonadal development period, methods are primarily used to simulate the low-temperature conditions naturally encountered by mandarin fish during their wintering, and to promote gonadal development in broodstock using hormones.

[0004] Therefore, the present invention needs to design a method for shortening the artificial propagation cycle of mandarin fish to solve the above problems. Summary of the Invention

[0005] The purpose of the present invention is to provide a method for shortening the artificial propagation period of mandarin fish in order to solve the above problems, thereby solving the problems mentioned in the background technology.

[0006] In order to solve the above problems, the present invention provides a technical solution:

[0007] A method for shortening the artificial propagation cycle of mandarin fish comprises the following steps:

[0008] S1. Construction of broodstock breeding system: Construct a broodstock breeding and aquaculture system for Siniperca chuatsi without natural light, equipped with full-spectrum lighting, water temperature control system and circulating water treatment system;

[0009] S2. Selecting broodstock for breeding: Selecting broodstock of Mandarin fish after breeding, marking them individually with RFID chips, measuring their body length and weight, and then separating the males and females and placing them in a broodstock breeding system for breeding;

[0010] S3. Temperature and light regulation to promote maturation: In a broodstock cultivation system without natural light, the low-temperature natural wintering conditions of Siniperca chuatsi were simulated, and the water temperature and light exposure cycle were set: the low-temperature natural wintering water temperature and light exposure cycle were set to 13°C and 10 hours, respectively. Female fish were kept at 13°C for 2 weeks, with the water temperature and light exposure cycle adjusted once a day. The water temperature was then raised to 23°C within 1 week, and the light exposure cycle was extended from 10 hours to 14 hours, and this was maintained for 5 weeks. Male fish were kept at 13°C for 4 weeks, with the water temperature and light exposure cycle adjusted once a day. The water temperature was then raised to 23°C within 1 week, and the light exposure cycle was extended from 10 hours to 14 hours, and this was maintained for 5 weeks.

[0011] S4. Screening of mature broodstock: Selecting mature broodstock individuals based on behavioral and morphological characteristics;

[0012] S5. Artificial insemination: Artificial insemination of mature broodstock to cultivate fry.

[0013] Preferably, in step S1, the broodstock cultivation system and the tools used in mandarin fish breeding are soaked and disinfected in a 3% potassium permanganate solution for 24 hours in advance.

[0014] Preferably, in step S1, the broodstock cultivation tank is 2m long, 2m wide, 1.6m deep, and has a water level of 1.2m. The broodstock cultivation tank is equipped with a circulating water aquaculture system. The cultivation water body is circulating water, which is supplied by the indoor circulating water aquaculture system.

[0015] Preferably, the indoor circulating aquaculture system includes eight parts: breeding equipment, filtering equipment, a water reservoir, a plant purification pool, a microbial treatment pool, a sterilization device, a water temperature control system and an LED light source control device. The water in the breeding equipment is filtered by the filtering equipment and enters the water reservoir. Then, the water enters the plant purification pool and the microbial treatment pool in turn for treatment. Finally, the water is sterilized by the sterilization device and then enters the broodstock breeding barrel. The water temperature control system controls the indoor circulating water temperature, and the LED light source control device controls the indoor light color and light intensity.

[0016] Preferably, the water temperature control system includes a heating pump and a thermostat, the indoor circulating water temperature is adjusted by the heating pump, and the thermostat is used for constant temperature control; an LED full-spectrum lamp is provided above the breeding pond, the LED full-spectrum lamp is 1m-1.5m away from the water surface of the breeding pond, and the LED full-spectrum lamp is electrically connected to the light environment intelligent controller; when the LED full-spectrum lamp is turned on, the water body below the circulating water treatment system is not illuminated.

[0017] Preferably, the sexually mature Siniperca chuatsi broodstock selected in step S2 are of normal body shape, without deformity, healthy and uninjured, the female broodstock are 2 years old or older and weigh 0.6 kg or more, and the male broodstock are 2 years old or older and weigh 0.6 kg or more.

[0018] Preferably, the density of the broodstock in the broodstock cultivation system in step S2 is 10 kg / m3, and the broodstock are fed twice a day. According to the feeding habits of the mandarin fish, the feeding time is set to 8:00 and 17:00, and the feed is fed at 5% of the broodstock body weight.

[0019] Preferably, in step S4, the abdomen of the mature female fish is relatively swollen, and when pressed lightly with the hand, the abdomen is soft and elastic, and the genital pore is slightly red; the genital pore of the mature male fish is loose, and milky white semen flows out when the abdomen is pressed lightly, and the semen can disperse naturally immediately after entering the water.

[0020] The beneficial effects of the present invention are as follows:

[0021] 1. Due to the use of factory-scale recirculating aquaculture system, the broodstock cultivation environment is controllable compared to outdoor cultivation ponds;

[0022] 2. The use of LED lights can simulate the light environment of natural reproduction of broodstock, and promote gonadal development by simulating the low-temperature natural wintering environment of mandarin fish and irradiating with full-spectrum LED lights;

[0023] 3. The use of LED light intelligent controller and temperature automatic controller reduces the interference of breeding environment and human activities on broodstock and reduces the stress response of broodstock. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] For ease of explanation, the present invention is described in detail with reference to the following specific implementations and accompanying drawings.

[0025] Figure 1 This is a structural diagram of a temperature-light-induced ripening system for mandarin fish broodstock, which is a method for shortening the artificial propagation period of mandarin fish according to the present invention;

[0026] Figure 2 This is a schematic diagram of a temperature and light ripening regulation program for mandarin fish of the present invention, in a method for shortening the artificial propagation period of mandarin fish;

[0027] Figure 3 This is a schematic diagram of the changes in GSI and serum estradiol levels of female Siniperca chuatsi in a method for shortening the artificial reproduction period of Siniperca chuatsi of the present invention;

[0028] Figure 4 Schematic diagram of changes in GSI and serum testosterone levels of male Siniperca chuatsi according to a method for shortening the artificial breeding cycle of Siniperca chuatsi of the present invention;

[0029] Figure 5 The present invention is a schematic diagram of gonadal tissue sections of Siniperca chuatsi broodstock in a method for shortening the artificial reproduction cycle of Siniperca chuatsi.

[0030] Reference numerals:

[0031] In the figure, 1. LED full-spectrum lamp; 2. Light environment intelligent controller; 3. Circulating water treatment system;

[0032] 4. Breeding pond; 5. Water temperature control system. DETAILED DESCRIPTION

[0033] like Figure 1-Figure 5 As shown, this specific embodiment adopts the following technical solutions:

[0034] A method for shortening the artificial propagation cycle of mandarin fish comprises the following steps:

[0035] S1. Constructing a broodstock cultivation system: A broodstock cultivation and breeding system for mandarin fish (Siniperca chuatsi) without natural light exposure was constructed, equipped with a full-spectrum illumination lamp 1, a water temperature control system 5, and a circulating water treatment system 3. The broodstock cultivation system and tools used in mandarin fish breeding were pre-soaked and disinfected in a 3% potassium permanganate solution for 24 hours. The broodstock cultivation tank was 2 meters long, 2 meters wide, and 1.6 meters deep, with a water level of 1.2 meters. The broodstock cultivation tank was equipped with a recirculating aquaculture system, and the cultivation water was recycled water supplied by the indoor recirculating aquaculture system.

[0036] S2. Selecting broodstock for breeding: Selecting broodstock of mandarin fish after breeding, using RFID chips for individual marking, measuring body length and body weight, separating males and females into broodstock breeding systems for breeding, selecting sexually mature mandarin fish with normal body shape, no deformities, and good health without injuries, female broodstock are 2 years old or older and weigh 0.6 kg or more, and male broodstock are 2 years old or older and weigh 0.6 kg or more, and the density of broodstock breeding system is 10 kg / m3, and the broodstock are fed twice a day. According to the feeding habits of mandarin fish, the feeding time is set at 8:00 and 17:00, and the feed is fed according to 5% of the body weight of the broodstock;

[0037] S3. Temperature and light regulation to promote maturation: In a broodstock cultivation system without natural light, the low-temperature natural wintering conditions of Siniperca chuatsi were simulated, and the water temperature and light exposure cycle were set: the low-temperature natural wintering water temperature and light exposure cycle were set to 13°C and 10 hours, respectively. Female fish were kept at 13°C for 2 weeks, with the water temperature and light exposure cycle adjusted once a day. The water temperature was then raised to 23°C within 1 week, and the light exposure cycle was extended from 10 hours to 14 hours, and this was maintained for 5 weeks. Male fish were kept at 13°C for 4 weeks, with the water temperature and light exposure cycle adjusted once a day. The water temperature was then raised to 23°C within 1 week, and the light exposure cycle was extended from 10 hours to 14 hours, and this was maintained for 5 weeks.

[0038] S4. Screening mature broodstock: Select mature broodstock based on their behavioral and morphological characteristics. Mature females have a relatively swollen abdomen that is soft and elastic when gently pressed with the hand, and a slightly reddish genital pore. Mature males have a relaxed genital pore and milky white semen flows out when the abdomen is gently pressed. The semen disperses naturally upon entering the water.

[0039] S5. Artificial insemination: Artificial insemination of mature broodstock to cultivate fry.

[0040] In this scheme, the indoor circulating aquaculture system includes 8 parts: breeding equipment, filtering equipment, a water reservoir, a plant purification pool, a microbial treatment pool, a sterilization device, a water temperature control system 5, a breeding pool 4, a light environment intelligent controller 2 and an LED light source control device. The water in the breeding equipment is filtered by the filtering equipment and enters the water reservoir. Then, the water enters the plant purification pool and the microbial treatment pool in turn for treatment. Finally, the water is sterilized by the sterilization device and then enters the broodstock breeding barrel. The water temperature control system 5 controls the indoor circulating water temperature, and the LED light source control device controls the indoor light color and light intensity.

[0041] In this solution, the water temperature control system 5 includes a heating pump and a thermostat. The indoor circulating water temperature is adjusted by the heating pump, and the thermostat is used for constant temperature control. An LED full-spectrum lamp 1 is provided above the aquaculture pond 4. The LED full-spectrum lamp 1 is 1m-1.5m away from the water surface of the aquaculture pond 4. The LED full-spectrum lamp 1 is electrically connected to the light environment intelligent controller 2. When the LED full-spectrum lamp 1 is turned on, the water body below the circulating water treatment system 3 is not illuminated.

[0042] Example 1

[0043] The process of the present invention is as follows:

[0044] 1. Materials and Methods

[0045] S1. Construction of a temperature-light-induced ripening system for Siniperca chuatsi broodstock:

[0046] like Figure 1As shown, a temperature-light-assisted maturation system for broodstock of mandarin fish of Culex chuatsi includes a breeding pond without natural light, a circulating water treatment system is provided above the water surface of the breeding pond, a water temperature control device is provided inside the breeding pond, and the water temperature control device is electrically connected to the water temperature intelligent controller outside the breeding pond; an LED full-spectrum lamp is provided above the breeding pond, the LED full-spectrum lamp is 1m away from the water surface of the breeding pond, and the LED full-spectrum lamp is electrically connected to the light environment intelligent controller; when the LED full-spectrum lamp is turned on, the water body below the circulating water treatment system is not illuminated. The water temperature control system includes a heating pump and a thermostat. The circulating water treatment system includes a water inlet and drainage system, a water treatment system, an oxygenation system and a water temperature online monitoring system. The broodstock breeding tank is 2m long, 2m wide, 1.6m deep, and the water level is 1.2m deep; the dissolved oxygen is above 6mg·L-1;

[0047] S2. Selecting broodstock of Siniperca chuatsi for breeding:

[0048] On October 11, 2023 (one week after breeding), a total of 40 pairs of male and female fish were selected after breeding. After individual marking and measurement, each group of 8 pairs was placed in 5 broodstock breeding ponds for breeding. The selected broodstock were normal in shape, healthy and without injuries. Females were 2 years old or older and weighed more than 0.6 kg, and males were 2 years old or older and weighed more than 0.6 kg. The marking method was to inject an electronic chip into the back muscle of the broodstock for individual marking. At the same time, a barcode scanner was used to read the chip number and measure and record the body length and weight of each individual. The broodstock breeding density was 8-10 kg / m3;

[0049] S3. Temperature and light regulation to promote ripening:

[0050] Simulate the low-temperature natural wintering conditions of Siniperca chuatsi, set water temperature and light cycle control programs to promote the maturation of broodstock gonads, use cooling and heating machines to raise or lower the water temperature to reach the set control temperature, use automatic temperature controllers to adjust to maintain a constant water temperature, or use air conditioning to lower the temperature and heating rods to raise the temperature;

[0051] The broodstock cultivation pools No. 1, No. 2, No. 3 and No. 4 for Culex chuatsi are identical. The Culex chuatsi broodstock cultivation system for temperature and light-induced ripening is constructed in step S1. The Culex chuatsi broodstock cultivation pools No. 1, No. 2 and No. 3 simulate the low-temperature natural wintering environment of Culex chuatsi, and set the water temperature and light cycle control program ( Figure 3The temperature environment used aerated tap water at 13°C as the water source. A heating rod was used to increase the temperature, and an automatic temperature controller was used to set and maintain the specific water temperature with a control accuracy of 0.5°C. The illumination area was provided by a full-spectrum LED light, each located 1m from the water surface, with a maximum light intensity of 10lx at the water surface. The LED light was connected to an intelligent light environment controller to automatically control on / off times and light intensity. The low-temperature, natural wintering water temperature and light duration were set at 13°C and 10 hours, respectively. Pool 1 maintained a low temperature of 13°C for two weeks, with the water temperature and light cycle adjusted daily. The water temperature was then raised to 23°C within one week, and the light duration was extended from 10 hours to 14 hours. Pool 2 maintained a low temperature of 13°C for four weeks, with the water temperature and light cycle adjusted daily. The water temperature was then raised to 23°C within one week, and the light duration was extended from 10 hours to 14 hours. Pond No. 3 was kept at a low temperature of 13℃ for 6 weeks, and the water temperature and light cycle were adjusted once a day. Then the water temperature was controlled to rise to 23℃ within 1 week, and the light duration was extended from 10h to 14h. The temperature of Pond No. 4 was controlled at 23±1℃, and the light duration was 14h per day. Pond No. 5 was an adult fish breeding pond in the recirculating aquaculture workshop, with a temperature controlled at 23±1℃ and a light cycle of natural light cycle ( Figure 2 The broodstock were fed artificially twice daily at 8:00 and 17:00 according to the feeding habits of Siniperca chuatsi. The broodstock were fed feed at a rate of 5% of their body weight. The female broodstock in pool 4 were fed a feed containing 10 mg / kg estradiol, while the male broodstock were fed a feed containing 10 mg / kg testosterone.

[0052] S4. Screening mature broodstock:

[0053] Based on the behavioral and morphological characteristics of the broodstock, the maturity of the broodstock was determined. On December 6, 2023, broodstock with slow swimming and swollen abdomens were selected from Pond No. 1 and placed in cages. Their body length and weight were measured. Based on their behavioral characteristics and genital pore color, six female broodstock were selected for spawning. They were temporarily housed in different cages and placed in Pond No. 1. On December 20, 2023, broodstock with slow swimming and swollen abdomens were selected from Pond No. 2 and placed in cages. Their body length and weight were measured. Based on their behavioral characteristics and genital pore color, five male broodstock were selected for spawning.

[0054] S5. Artificial insemination:

[0055] Artificial insemination was performed with reference to the technical specifications for artificial propagation of Siniperca chuatsi. A single injection into the pectoral fin axilla was used to induce labor. The type and dosage of the oxytocin are detailed in Table 1. The interval between injections was 12 days. The water temperature was controlled at 24-27°C, and the effect lasted for 23-28 hours. Dry fertilization was used. First, the egg container and the surface of the female broodstock were wiped dry. The fish was tilted, and the genital pore was aligned with the container. The eggs were gently pressed on the fish belly with the hand, and the container flowed into the container. At the same time, the male semen was squeezed in using the same method, or semen was sucked up with a straw and dripped onto the eggs. A feather was used to stir the semen evenly so that all the eggs were in contact with it. After standing for 1-2 minutes, clean water was added, stirred slightly, and the mixture was allowed to stand for another 1-2 minutes. Then, the mixture was rinsed with water several times, and the fertilized eggs were placed in an incubator for incubation.

[0056] (6) Sampling:

[0057] The experiment started on October 11, 2023 and lasted for 13 weeks. On October 11, 2023, 2-3 mL of blood was collected from the tail vein for the first time using a syringe. Thereafter, blood was collected every two weeks using the same method and placed in a refrigerated centrifuge tube. The tube was centrifuged at 4000 rpm for 20 minutes, and the supernatant was collected and stored at -80°C. The estradiol concentration in the serum of female fish was determined using an ELISA kit, and the testosterone concentration in the serum of male fish was determined. The gonads of mandarin fish were sampled at the same time points to measure the GSI index.

[0058] 2. Experimental Results

[0059] Gonad index = (gonad weight / fish body weight) * 100%. The concentrations of estradiol and testosterone in serum were determined using ELISA kits.

[0060] S1. Under the conditions of Example 1, from the start of the experiment on October 11, 2023 to the end of the experiment on December 5, 2023, the gonad index of the female broodstock in Pool 1 gradually increased from 0.97% to 5.60%, and the gonad index of the female broodstock in Pool 2 gradually increased from 0.96% to 3.32%. The gonad index of the female broodstock in Pool 3 gradually increased from 0.95% to 2.32%, and the gonad index of the female broodstock in Pool 4 gradually increased from 0.96% to 5.93%. The gonad index of the female broodstock in Pool 5 did not change significantly throughout the experimental period and was 0.97% ( Figure 3 );

[0061] Under the conditions of Example 1, from the start of the experiment on October 11, 2023 to the end of the experiment on December 5, 2023, the estradiol content in the serum of the female broodstock in Pool 1 gradually increased from 275.41 pg / mL to 892.55 pg / mL, and according to Figure 5The eggs were mature as observed in section B. The estradiol content in the serum of the female broodstock in pool 2 gradually increased from 272.78 pg / mL to 618.70 pg / mL, the estradiol content in the serum of the female broodstock in pool 3 gradually increased from 271.36 pg / mL to 435.11 pg / mL, and the estradiol content in the serum of the female broodstock in pool 4 gradually increased from 269.38 pg / mL to 940.07 pg / mL. The estradiol content in the serum of the female broodstock in pool 5 did not change significantly throughout the experimental period and was 273.78 pg / mL at the end of the experiment ( Figure 3 );

[0062] Under the conditions of Example 1, the gonad index of the male broodstock in pool 1 gradually increased from 0.71% to 1.88% from the start of the experiment on October 11, 2023 to the end of the experiment on December 5, 2023; the gonad index of the male broodstock in pool 2 gradually increased from 0.71% to 4.62% from the start of the experiment on October 11, 2023 to the end of the experiment on December 19, 2023; the gonad index of the male broodstock in pool 3 gradually increased from 0.71% to 4.62% from the start of the experiment on October 11, 2023 to the end of the experiment on December 19, 2023; From the beginning of the experiment to the end of January 2, 2024, it gradually increased from 0.72% to 4.74%. The gonadal index of male broodstock in pool 4 increased from 0.96% to 5.93% from the beginning of the experiment on October 11, 2023 to the end of December 5, 2023. The gonadal index of male broodstock in pool 5 increased from 0.71% to 2.38% from the beginning of the experiment on October 11, 2023 to the end of the experiment on December 5, 2023. Figure 4 );

[0063] Under the conditions of Example 1, the testosterone content in the serum of male broodstock in pool 1 gradually increased from 193.12 pg / mL to 387.24 pg / mL from the start of the experiment on October 11, 2023 to the end of the experiment on December 5, 2023. The testosterone content in the serum of male broodstock in pool 2 gradually increased from 193.79 pg / mL to 635.13 pg / mL from the start of the experiment on October 11, 2023 to the end of the experiment on December 19, 2023. Figure 5D section observation showed that the sperm had matured. The testosterone content in the serum of male broodstock in pool 3 gradually increased from 192.54 pg / mL to 643.27 pg / mL from the start of the experiment on October 11, 2023 to the end of the experiment on January 2, 2024. The testosterone content in the serum of male broodstock in pool 4 gradually increased from 191.54 pg / mL to 665.34 pg / mL from the start of the experiment on October 11, 2023 to the end of the experiment on December 5, 2023. The testosterone content in the serum of male broodstock in pool 5 gradually increased from 193.11 pg / mL to 469.64 pg / mL from the start of the experiment on October 11, 2023 to the end of the experiment on December 5, 2023. Figure 4 ).

[0064] Compared with the existing technology, the present invention has the following beneficial effects: it shortens the breeding cycle of broodstock, simulates the low-temperature natural wintering environment of mandarin fish, sets the water temperature and the illumination cycle of the illumination lamp, stimulates the gonads of broodstock to develop again, and can advance the spawning time of mandarin fish (mid-April) by 3 months.

[0065] Figure 5 A: Immature ovary tissue section; B: mature ovary tissue section; C: immature testis tissue section; D: mature testis tissue section.

[0066] Table 1 Injection method for induced spawning of Siniperca chuatsi broodstock

[0067] drug LHR-A2 (ug / kg) DOM (mg / kg) HCG (IU / kg) Dosage 6.25 2.5 500

[0068] Those skilled in the art will appreciate that the modules and method steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professionals and technicians can use different methods to implement the described functions for each specific application, but such implementation should not be considered beyond the scope of the present invention.

[0069] Those skilled in the art will clearly understand that, for the convenience and brevity of description, the specific working processes of the above-described devices, equipment and modules can refer to the corresponding processes in the aforementioned method embodiments and will not be repeated here.

[0070] In addition, each functional module in each embodiment of the present invention may be integrated into one processing module, or each module may exist physically separately, or two or more modules may be integrated into one module.

[0071] In addition, it should be noted that the combination of the various technical features in this case is not limited to the combination described in the claims of this case or the combination described in the specific embodiments. All technical features recorded in this case can be freely combined or combined in any way unless there is a contradiction between them.

[0072] It should be noted that the above examples are merely specific embodiments of the present invention. Obviously, the present invention is not limited to the above examples, and many similar variations are possible. All variations directly derived from or associating with the present invention by those skilled in the art are intended to fall within the scope of protection of the present invention.

[0073] The above are only preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for shortening the artificial propagation period of mandarin fish, characterized in that: The steps include: S1. Constructing a broodstock breeding system: constructing a broodstock breeding and aquaculture system for Siniperca chuatsi without natural light exposure, equipped with a full-spectrum illumination lamp (1), a water temperature control system (5), and a circulating water treatment system (3); S2. Selecting broodstock for breeding: Selecting broodstock of Mandarin fish after breeding, marking them individually with RFID chips, measuring their body length and weight, and then separating the males and females and placing them in a broodstock breeding system for breeding; S3. Temperature and light regulation to promote maturation: In a broodstock cultivation system without natural light, the low-temperature natural wintering conditions of Siniperca chuatsi were simulated, and the water temperature and light exposure cycle were set: the low-temperature natural wintering water temperature and light exposure cycle were set to 13°C and 10 hours, respectively. Female fish were kept at 13°C for 2 weeks, with the water temperature and light exposure cycle adjusted once a day. The water temperature was then raised to 23°C within 1 week, and the light exposure cycle was extended from 10 hours to 14 hours, and this was maintained for 5 weeks. Male fish were kept at 13°C for 4 weeks, with the water temperature and light exposure cycle adjusted once a day. The water temperature was then raised to 23°C within 1 week, and the light exposure cycle was extended from 10 hours to 14 hours, and this was maintained for 5 weeks. S4. Screening of mature broodstock: Selecting mature broodstock individuals based on behavioral and morphological characteristics; S5. Artificial insemination: Artificial insemination of mature broodstock to cultivate fry.

2. The method for shortening the artificial propagation cycle of mandarin fish according to claim 1, wherein: In step S1, the broodstock breeding system and the tools used in mandarin fish breeding are immersed and disinfected in a 3% potassium permanganate solution for 24 hours in advance.

3. The method for shortening the artificial propagation cycle of mandarin fish according to claim 1, wherein: In step S1, the broodstock cultivation tank is 2m long, 2m wide, 1.6m deep, and has a water level of 1.2m. The broodstock cultivation tank is equipped with a circulating water aquaculture system. The cultivation water is circulating water supplied by the indoor circulating water aquaculture system.

4. A method for shortening the artificial propagation period of mandarin fish according to claim 3, characterized in that: The indoor circulating water aquaculture system comprises eight parts, namely, aquaculture equipment, filtering equipment, a water reservoir, a plant purification pool, a microbial treatment pool, a sterilizing device, a water temperature control system (5), aquaculture pool (4), a light environment intelligent controller (2), and an LED light source control device. The water in the aquaculture equipment is filtered by the filtering device and then enters the water reservoir. The water then enters the plant purification pool and the microbial treatment pool in sequence for treatment. Finally, the water is sterilized by the sterilizing device and then enters the parent fish culture barrel. The water temperature control system (5) controls the indoor circulating water temperature, and the LED light source control device controls the indoor light color and light intensity.

5. The method for shortening the artificial propagation cycle of mandarin fish according to claim 4, wherein: The water temperature control system (5) includes a heating pump and a thermostat, and the indoor circulating water temperature is adjusted by the heating pump, and the thermostat is used for constant temperature control; an LED full-spectrum lamp (1) is provided above the aquaculture pond (4), and the LED full-spectrum lamp (1) is 1m-1.5m away from the water surface of the aquaculture pond (4), and the LED full-spectrum lamp (1) is electrically connected to the light environment intelligent controller (2); when the LED full-spectrum lamp (1) is turned on, the water body below the circulating water treatment system (3) is not illuminated.

6. The method for shortening the artificial propagation cycle of mandarin fish according to claim 1, wherein: The sexually mature Siniperca chuatsi broodstock selected in step S2 are of normal body shape, without deformity, and healthy without injury. The female broodstock are 2 years old or older and weigh 0.6 kg or more, and the male broodstock are 2 years old or older and weigh 0.6 kg or more.

7. The method for shortening the artificial propagation period of mandarin fish according to claim 6, wherein: The density of the broodstock in the broodstock cultivation system in step S2 is 10 kg / m3, and the broodstock are fed twice a day. According to the feeding habits of the mandarin fish, the feeding time is set to 8:00 and 17:00, and the feed is fed at 5% of the broodstock body weight.

8. The method for shortening the artificial propagation period of mandarin fish according to claim 1, wherein: In step S4, the abdomen of the mature female fish is relatively swollen. When the abdomen is gently pressed with the hand, it is soft and elastic, and the genital pore is slightly red. The genital pore of the mature male fish is loose, and milky white semen flows out when the abdomen is gently pressed. After the semen enters the water, it can immediately disperse naturally.

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