Method and equipment for purifying glassine from enzymatic hydrolysate

Abstract

The invention belongs to the technical field of solid-liquid separation, and particularly relates to a method and equipment for purifying glassine from enzymatic hydrolysate, and the method comprises the following steps: S1, crushing and degreasing raw materials, washing the degreased raw materials with clear water, removing residual solvents and water-soluble impurities, and drying the degreased raw materials with drying equipment until the water content is lower than 10%; the method comprises the following steps: quickly releasing high-pressure steam to destroy the cell structure of a raw material, adding deionized water into the raw material and water according to a preset ratio of (1: 10)-(1: 20), and uniformly stirring to form a substrate suspension; according to the purification process, through a combined strategy of physical separation, adsorption purification and precise separation, the balance of process efficiency, cost control and environmental friendliness is realized while high purity and biological activity of the glass color are ensured, and the purification process is particularly suitable for large-scale preparation of functional micromolecular active components from plant enzymatic hydrolysate.

Description

Technical Field

[0001] The invention belongs to the technical field of solid-liquid separation, and in particular relates to a method and equipment for purifying boson from an enzymatic hydrolyzate. Background Art

[0002] Bosphorin, a common cosmetic ingredient, is chemically known as hydroxypropyl tetrahydropyranol. It promotes collagen production and improves skin elasticity. Originally extracted from beech trees or cassava starch, it is a naturally derived ingredient. Compared to some chemically synthesized skincare ingredients, naturally derived bosphorin is more easily accepted by the skin, is safer, and better meets consumers' demand for natural, gentle skincare products. Extracting active ingredients from natural plants is also a manifestation of sustainable development, contributing to the protection of the ecological environment and biodiversity.

[0003] Extracting bosphorin from plants requires dissolution, filtration, and concentration. Filters are often used in the filtration process. The filter forms a filtration channel between the plates and frames, using pressure to force the liquid in the extract through the filter cloth while retaining solid impurities on the cloth, thereby achieving solid-liquid separation and obtaining a clear bosphorin extract. During the filtration process, solid particles gradually accumulate on the surface and in the pores of the filter cloth, causing the cloth to clog and the filtration rate to gradually decrease. Increasing the pressure to maintain a certain filtration rate may damage the filter cloth, causing solid impurities to enter the extract and reducing the purity of the bosphorin.

[0004] In addition, in addition to polysaccharides, beech wood chips also contain lignin, hemicellulose, resin and other components; during the polysaccharide extraction process, some of these components may remain in the residue; among them, resinous substances and some polysaccharides that have not been completely extracted are sticky, which will make the residue have a certain stickiness; when trying to tear off large pieces of residue stuck to the filter cloth, due to the strong adhesion between the residue and the filter cloth, a large external force is required to separate them; if the external force is too large and exceeds the strength that the filter cloth can withstand, it may cause the filter cloth to tear or crack; especially when the filter cloth has been used for a long time and its strength has decreased, it is more likely to tear. Summary of the Invention

[0005] In order to make up for the deficiencies of the prior art and solve the above-mentioned technical problems, the present invention proposes a method and equipment for purifying boson from an enzymatic hydrolyzate.

[0006] The technical solution adopted by the present invention to solve the technical problem is: the present invention proposes a method for purifying bosine from an enzymatic hydrolyzate, the purification method comprising:

[0007] S1: The raw material is crushed and defatted, and after degreasing, the raw material is washed with clean water to remove residual solvent and water-soluble impurities, and then dried by drying equipment until the water content is less than 10%; the cell structure of the raw material is destroyed by rapid release of high-pressure steam, and deionized water is added according to a preset ratio of raw material to water, including 1:10-1:20, and stirred to form a substrate suspension;

[0008] S2: adding specific enzymes, including cellulase, to the suspension to produce an enzymatic hydrolyzate through enzymatic hydrolysis; the initially obtained enzymatic hydrolyzate is heated to inactivate the enzyme, filtered through a filter and adsorbed on activated carbon to remove impurities, and then concentrated under reduced pressure and adjusted in pH to obtain an enzymatic hydrolyzate for extraction;

[0009] S3: The enzymatic hydrolysate is subjected to resin adsorption, using a macroporous adsorption resin, which is pretreated and then loaded onto a chromatographic column. The concentrated enzymatic hydrolysate is loaded onto a sample at a predetermined flow rate, impurities are eluted with deionized water, and then phosphene is eluted with a 30%-50% ethanol solution, and the eluate is collected.

[0010] S4: Add activated carbon to the eluate, stir at room temperature, and filter to remove trace pigments; remove residual inorganic salts through a cation exchange resin and anion exchange resin in series to obtain a refined liquid; concentrate the refined liquid under reduced pressure below 40°C to a solid content of 10%-20%; dry the concentrated liquid into a white powder in a spray drying tower to obtain bosphorin powder.

[0011] A device for purifying boson from an enzymatic hydrolyzate, comprising a filter, which comprises a filter body, a pressing device, a filter frame, a filter cloth, a feeding device, and a discharging device; the filter also comprises:

[0012] Electric guide rails are installed on both sides of the filter body, and a slider is slidably connected to the electric guide rails. A paddle is hinged to the top of the slider through a torsion spring. A sensor is provided on one side of the paddle that contacts the surface of the filter frame. A bracket is installed on the slider, and shovel blocks are slidably connected to both sides of the bracket. The bottom of the shovel blocks is tilted toward the filter cloth. One side of the two shovel blocks contacts the filter cloth surface on both sides of the filter frame respectively. The shovel blocks are connected to the electric push rods on the bracket.

[0013] The water supply unit is installed at one end of the filter body. A water injection pipe is connected to the bracket through a spring sliding connection. The water injection pipe is connected to the water nozzle on the filter frame. Both sides of the water injection pipe are in contact with the shovel block. A water extraction pipe is provided in the shovel block. The water extraction pipe contacts the filter cloth. The water supply unit supplies water to the water injection pipe and extracts water to the water extraction pipe.

[0014] Preferably, the water injection pipe is a four-way tube, the top of the water injection pipe is connected to the water supply unit through a water pipe, the bottom of the water injection pipe is provided with a valve and the bottom is connected to the inside of the filter frame, the bracket is located on both sides of the water injection pipe and is connected to the sealing block through a spring sliding, and the bottom of the sealing block is in contact with the shovel block; when the shovel block rises to lift the sealing block, the water injection pipe is connected to the water pumping pipe, and when the shovel block descends and drives the sealing block to descend and recover, the water injection pipe is away from the water pumping pipe, and the openings on both sides of the water injection pipe are blocked by the sealing blocks.

[0015] Preferably, a cleaning groove is provided on the side of the shovel block close to the filter cloth, and a water suction pipe is located above the cleaning groove, and the water suction pipe contacts the filter cloth through a rubber ring provided at the opening; a cleaning rod is rotatably connected in the cleaning groove, and the cleaning rod is connected to the motor provided in the shovel block, and a cleaning brush is provided on the cleaning rod.

[0016] Preferably, a cleaning plate is distributed in an annular manner on the cleaning rod, and the cleaning plate is hinged to the surface of the cleaning rod through a torsion spring. When the cleaning plate rotates to the top of the cleaning rod, it is squeezed and tilted by the inner wall of the cleaning groove.

[0017] Preferably, the bottom of the shovel block is connected to a swing rod through a spring sliding connection, one end of the swing rod is rotatably connected to a roller, the outer ring of the roller contacts the filter cloth, and when the cleaning plate rotates to the bottom of the cleaning rod away from the filter cloth, it contacts and moves the swing rod.

[0018] Preferably, protrusions are evenly provided on the outer ring of the roller, and rubber strips are evenly provided on the surface of the protrusion away from the roller, and the rubber strips are arranged horizontally.

[0019] Preferably, a storage rod is installed on the filter body, and the water pipes on the water injection pipe and the water extraction pipe connected to the water supply unit are wrapped around the storage rod; when the water pipes are working, they are retracted to the position of the storage rod close to the water supply unit, and when the water pipes are cleaned or left stationary, the water pipes are inclined downward toward the water supply unit.

[0020] Preferably, a coil spring is wound around the water pipes used for connecting the water injection pipe and the water extraction pipe.

[0021] Preferably, a marking device is provided at one end of the shovel block, and the marking device contacts the side edge of the filter frame, and the marking device is used to mark the edge of the filter frame where the filter cloth is located.

[0022] The beneficial effects of the present invention are as follows:

[0023] 1. The method and equipment for purifying bosera from an enzymatic hydrolyzate described in the present invention, wherein a shovel is lowered and inserted between the filter cake and the filter cloth to peel the two off. Compared with the existing method of directly scraping or simply beating the filter cake, the method of removing the filter cake by peeling off can effectively protect the filter cloth, avoid damage to the filter cloth when cleaning the filter cake, and avoid the appearance of cracks or hidden cracks on the filter cloth, thereby improving the filtration effect and further improving the extraction and purification effect of bosera.

[0024] 2. The method and equipment for purifying boson from an enzymatic hydrolyzate described in the present invention, the water injection pipe slides down on the bracket through the elasticity of the spring and is connected to the water nozzle on the filter frame, the water supply unit injects cleaning liquid into the filter frame through the water injection pipe, the shovel drives the water pumping pipe to drop to contact the filter cloth, the water supply unit sucks the cleaning liquid into the filter cloth through the water pumping pipe, the cleaning liquid penetrates the filter cloth in the reverse direction, and washes the impurities on the filter cloth out of the filter frame along the water pumping pipe, completing the cleaning of the filter frame and the filter cloth. Through the combined effect of the above-mentioned pressure-applied flushing of the filter cloth and the suction of the filter cloth, the efficiency of the cleaning liquid penetrating the filter cloth is improved, the cleaning time is shortened, the filtration efficiency is improved, and the extraction and purification efficiency of boson is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The present invention will be further described below with reference to the accompanying drawings.

[0026] Figure 1 is a flow chart of the method steps of the present invention;

[0027] Figure 2 is a perspective view of the present invention;

[0028] Figure 3 It is a partial cross-sectional view of the present invention in the side view direction;

[0029] Figure 4 It is a cross-sectional view from the side when the shovel block is lowered;

[0030] Figure 5 It is a cross-sectional view from the side when the shovel block is rising;

[0031] Figure 6 This is a schematic diagram of the slider driving the filter frame to separate through the dial;

[0032] Figure 7 It is a cross-sectional view of the bracket in a three-dimensional state;

[0033] Figure 8 This is the state diagram when the shovel block detects the filter cloth;

[0034] Figure 9 This is the state diagram when the water injection pipe and the water extraction pipe are connected;

[0035] Figure 10 This is a state diagram when the water injection pipe and the cleaning tank are connected;

[0036] Figure 11 It is a three-dimensional diagram of the water injection pipe.

[0037] In the figure: filter body 1, pressing device 11, filter frame 12, filter cloth 13, feeding device 14, discharging device 15, electric guide rail 16, slider 17, dial plate 18, sensor 19, bracket 2, shovel block 21, electric push rod 22, water supply unit 23, water injection pipe 24, water extraction pipe 25, sealing block 26, cleaning groove 27, rubber ring 28, cleaning rod 3, motor 31, cleaning brush 32, cleaning plate 33, swing rod 34, roller 35, protrusion 36, rubber strip 37, storage rod 38, coil spring 39, marking device 4. DETAILED DESCRIPTION

[0038] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings shown in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0039] Example 1:

[0040] In order to effectively solve the above problems, as shown in the accompanying drawings of the specification Figure 2-Figure 11 As shown, a boson extraction and purification device includes a filter, which consists of a filter body 1, a pressing device 11, a filter frame 12, a filter cloth 13, a feeding device 14 and a discharging device 15;

[0041] The filter also includes:

[0042] An electric guide rail 16 is mounted on both sides of the filter body 1. A slider 17 is slidably connected to the electric guide rail 16. A paddle 18 is hinged to the top of the slider 17 via a torsion spring. A sensor 19 is provided on one side of the paddle 18 that contacts the surface of the filter frame 12. A bracket 2 is mounted on the slider 17. Shovel blocks 21 are slidably connected to both sides of the bracket 2. The bottom of the shovel blocks 21 is tilted toward the filter cloth 13. One side of the two shovel blocks 21 contacts the surface of the filter cloth 13 on both sides of the filter frame 12. The shovel blocks 21 are connected to the electric push rod 22 on the bracket 2.

[0043] A water supply unit 23 is installed at one end of the filter body 1. A water injection pipe 24 is slidably connected to the bracket 2 through a spring. The water injection pipe 24 is connected to the water nozzle on the filter frame 12. Both sides of the water injection pipe 24 are in contact with the shovel block 21. A water extraction pipe 25 is provided in the shovel block 21. The water extraction pipe 25 contacts the filter cloth 13. The water supply unit 23 supplies water to the water injection pipe 24 and extracts water to the water extraction pipe 25.

[0044] The pressing device 11, filter frame 12, filter cloth 13, feeding device 14 and discharging device 15 are all conventional components of a filter. The pressing device 11 is a conventional component of a filter and is powered by a hydraulic system or a mechanical transmission device. For example, in a hydraulic system, an oil pump delivers hydraulic oil to an oil cylinder, pushing a piston, thereby driving the pressing plate to move. The feeding device 14 and the discharging device 15 are conventional combined devices of pipes and pumps.

[0045] The electric guide rail 16 is a conventional electric moving device, which drives the slider 17 to move back and forth on the guide rail; the sensor 19 is a conventional pressure sensor 19; the electric push rod 22 is a conventional electric telescopic device, and the water supply unit 23 is a conventional device with water supply and pumping functions, and the water supply unit 23 can act on the water pumping pipe 25 with a stable suction pressure;

[0046] Specific work flow: The working process of filter frame 12 filter press:

[0047] Feeding: The compression plate pushes each filter frame 12 together in sequence until the sealing surfaces between adjacent filter frames 12 are tightly fitted to form a closed filtering space; during this process, the filter cloth 13 between the filter frames 12 will be evenly compressed between the filter frames 12 to ensure that no leakage occurs during the filtration process; when the filter frames 12 are closed in place, the feeding device 14 starts to work. The feeding device 14, such as a centrifugal pump or a screw pump, conveys the beech wood chip raw material through the feeding pipe to each filter frame 12 of the filter press. The raw material enters the filtering space between the filter frames 12 under the action of pressure;

[0048] Press filtration: The raw materials entering the filter frame 12 begin to separate solids and liquids under the action of feed pressure. The filtrate can pass through the filter cloth 13, while solid particles such as beech wood chips are retained on the surface of the filter cloth 13. As the filtration progresses, the solid particles gradually accumulate on the surface of the filter cloth 13 to form a filter cake. The filtrate that passes through the filter cloth 13 passes through the discharge device 15, flows along the filtrate channel on the filter frame 12 to the filtrate outlet, and is then collected into the filtrate storage tank through a pipeline.

[0049] Unloading: After the filtration is completed, it is necessary to release the clamping device 11 first, and the oil cylinder in the hydraulic system retracts the piston by releasing pressure, driving the clamping plate to move in the direction away from the thrust plate, and gradually loosening the filter frame 12; when the clamping plate is loosened to a certain extent, the electric guide rail 16 drives the slider 17 to move. When the slider 17 passes the filter frame 12, the paddle plate 18 is squeezed and swings over, and the electric guide rail 16 drives the slider 17 to return; when the slider 17 drives the paddle plate 18 to return, the paddle plate 18 drives the sensor 19 to contact the edge of the filter frame 12. Since the paddle plate 18 is hinged, the paddle plate 18 can drive the filter frame 12 to return during the return stroke until the filter frame 12 is pulled open in turn, forming a unloading channel; after the filter frame 12 is pulled open, the filter cake formed by the beech wood chip raw material has a large viscosity and will stick to the filter cloth. With the help of the shovel block 21, the filter cake is dropped from the filter cloth 13 to achieve complete unloading;

[0050] Cleaning: After unloading is completed, in order to prepare for the next filtering operation, the filter frame 12 and the filter cloth 13 need to be cleaned. Conventional cleaning liquid is injected into the filter frame 12 through the cleaning pipe equipped in the conventional filter body 1. The cleaning liquid can be clean water, hot water or a solution containing chemical agents. It is selected according to the properties of the filtered material and the cleaning requirements. The filter frame 12 and the filter cloth 13 are cleaned with the cleaning liquid.

[0051] When the dial plate 18 drives the sensor 19 to contact the filter frame 12, the sensor 19 is triggered, and the filter body 1 controls the electric push rod 22 to extend and drive the shovel block 21 to descend. At this time, the slider 17 drives the shovel block 21 to move to both sides of the filter frame 12 through the bracket 2. The shovel block 21 descends and is inserted between the filter cake and the filter cloth 13 to peel the two apart. Compared with the existing method of direct scraping or simple knocking, the method of removing the filter cake by peeling can effectively protect the filter cloth 13, avoid the filter cloth 13 from being damaged when cleaning the filter cake, and avoid cracks or hidden cracks on the filter cloth 13, thereby improving the filtration effect and then improving the extraction and purification effect of boson;

[0052] When the shovel block 21 is stationary, the shovel block 21 is located above the filter frame 12, and the shovel block 21 drives the water injection pipe 24 to rise away from the water nozzle on the filter frame 12; when the shovel block 21 falls, the shovel block 21 no longer presses the water injection pipe 24, and the water injection pipe 24 slides down on the bracket 2 due to the elasticity of the spring and is connected to the water nozzle on the filter frame 12, and the water supply unit 23 injects cleaning liquid into the filter frame 12 through the water injection pipe 24, and the shovel block 21 drives the water extraction pipe 25 to fall until it contacts the filter cloth 13, and the water supply unit 23 sucks the cleaning liquid to the filter cloth 13 through the water extraction pipe 25, and the cleaning liquid penetrates the filter cloth 13 in the reverse direction, flushing the impurities on the filter cloth 13 and leaving the filter frame 12 along the water extraction pipe 25, completing the cleaning of the filter frame 12 and the filter cloth 13. Through the combined effect of the above-mentioned pressure washing of the filter cloth and the suction of the filter cloth, the efficiency of the cleaning liquid penetrating the filter cloth 13 is improved, the cleaning time is shortened, the filtration efficiency is improved, and the extraction and purification efficiency of boson is improved;

[0053] If the water suction pipe 25 is not used, the entire filter cloth 13 is subjected to the pressure of the water in the filter frame 12. Part of the filter cloth 13 is not blocked by impurities, while part is blocked by impurities. This makes the pressure distribution of the water on the filter cloth 13 uneven, resulting in excessive pressure on a part of the filter cloth 13, causing damage or cracks. Therefore, by providing the water suction pipe 25, the opening width of the water suction pipe 25 covers the two side edges of the filter cloth 13, and the opening length of the water suction pipe 25 is smaller than the length of the filter cloth 13, so that during the downward suction process of the water suction pipe 25, the entire filter cloth 13 can be gradually sucked with a fixed area, thereby distributing the pressure of flushing the filter cloth 13, improving the cleaning effect of the filter cloth 13, and improving the protection of the filter cloth 13.

[0054] Moreover, when the water pipe 25 is sucking, the shovel block 21 can also support the suction part of the filter cloth 13, thereby improving the support of the filter cloth 13 and preventing deformation due to suction.

[0055] Moreover, workers can choose to clean the filter cake first, and then clean the filter cloth 13 after the filter frame 12 is away from the filter cake collection position, so as to avoid the cleaning liquid from being contaminated with the filter cake and affecting the filter cake recovery;

[0056] In addition, during the suction process, the suction pipe can also assist workers in judging the blockage and damage of the filter cloth 13, for example:

[0057] Determine whether filter cloth 13 is clogged

[0058] Principle: Under normal circumstances, when reverse suction cleaning is performed, the flow of the cleaning liquid in the filter cloth 13 will be relatively smooth, the suction equipment can easily extract the cleaning liquid, the pressure change in the pipeline is small, and the flow rate can reach a certain value; if the filter cloth 13 is blocked, the pores of the filter cloth 13 will be filled with a large amount of solid particles, which will increase the resistance of the cleaning liquid to pass through the filter cloth 13;

[0059] Symptoms: At this time, the suction equipment requires greater suction force to extract the cleaning liquid, which will cause the negative pressure value in the suction pipe to increase and the flow rate to decrease significantly. By observing the operating parameters of the suction part of the water supply unit 23, such as the reading of the negative pressure gauge and the display of the flow meter, it can be determined whether the filter cloth 13 is clogged. For example, if the negative pressure value is much higher than that during normal cleaning, but the flow rate is far lower than the normal level, it can be basically determined that the filter cloth 13 is seriously clogged.

[0060] Determine if filter cloth 13 is damaged

[0061] Principle: When the filter cloth 13 is damaged, the flow path of the cleaning liquid will change during reverse suction cleaning. Since a larger channel is formed at the damaged part, the cleaning liquid will preferentially pass through the damaged part instead of evenly passing through the filter cloth 13.

[0062] Symptoms: This will lead to uneven flow distribution during the suction process, and there may be a sudden increase in local flow. At the same time, due to the smaller resistance at the damaged part, the negative pressure value required in the area corresponding to the damaged part will be relatively smaller than that at the undamaged part when sucking the same amount of cleaning liquid. This can be observed and determined from the operating parameters. In addition, if the filter cloth 13 is severely damaged, abnormal airflow or liquid flow sounds may be heard during the suction process. This is because the air or cleaning liquid generates irregular flow when passing through the damaged part.

[0063] Workers can also install a conventional flow meter in the water pumping pipe 25 to detect the water flow at a close distance near the water pumping port on the water pumping pipe 25, thereby improving detection accuracy and reducing interference with the pipeline.

[0064] Example 2:

[0065] On the basis of Example 1, the water injection pipe 24 is a four-way tube. The top of the water injection pipe 24 is connected to the water supply unit 23 through a water pipe. The bottom of the water injection pipe 24 is provided with a conventional valve and the bottom is connected to the inside of the filter frame 12. The bracket 2 is located on both sides of the water injection pipe 24 and is slidably connected to a sealing block 26 through a spring, and the bottom of the sealing block 26 is in contact with the shovel block 21; when the shovel block 21 rises to lift the sealing block 26, the water injection pipe 24 is connected to the water extraction pipe 25. When the shovel block 21 descends and drives the sealing block 26 to descend and recover, the water injection pipe 24 is away from the water extraction pipe 25, and the openings on both sides of the water injection pipe 24 are blocked by the sealing blocks 26;

[0066] A cleaning groove 27 is formed on one side of the shovel block 21 near the filter cloth 13. A water extraction pipe 25 is located above the cleaning groove 27. The water extraction pipe 25 contacts the filter cloth 13 through a rubber ring 28 provided at the opening. A cleaning rod 3 is rotatably connected to the cleaning groove 27. The cleaning rod 3 is connected to a motor 31 provided in the shovel block 21. A cleaning brush 32 is provided on the cleaning rod 3.

[0067] The cleaning rod 3 is provided with a cleaning plate 33 in an annular arrangement. The cleaning plate 33 is hinged to the surface of the cleaning rod 3 by a torsion spring. When the cleaning plate 33 rotates to the top of the cleaning rod 3, it is squeezed and tilted by the inner wall of the cleaning groove 27.

[0068] The rubber ring 28 is a conventional type with a low friction coefficient and a soft texture to prevent excessive friction between the rubber ring 28 and the filter cloth 13 from causing wear on the filter cloth 13. The motor 31 is a conventional type with a waterproof function. The cleaning brush is a conventional tool for cleaning the filter cloth 13. A cleaning plate 33 is provided on the surface of the cleaning rod 3 between each two adjacent cleaning brushes 32.

[0069] Specific working process: When the water injection pipe 24 is stationary, the shovel block 21 pushes open the sealing block 26, and the sealing block 26 slides upward, the water injection pipe 24 and the water extraction pipe 25 are connected, and the valve at the bottom of the water injection pipe 24 is closed. After the water supply unit 23 injects water into the water injection pipe 24, the water flows back directly through the water extraction pipe 25 to clean the inside of the water extraction pipe 25 and the water pipe, so as to prevent the water extraction pipe 25 from being clogged by impurities or affecting the flow rate, thereby affecting the workers' auxiliary judgment of the condition of the filter cloth 13, thereby improving practicality;

[0070] When the water injection pipe 24 is working, the shovel block 21 is away from the water injection pipe 24, and the sealing block 26 is lowered and reset by the spring, and the two sides of the water injection pipe 24 are re-blocked. The valve at the bottom of the water injection pipe 24 is opened, and the water injection pipe 24 injects water into the filter frame 12 to complete the cleaning work; through the cooperation of the sealing block 26 and the valve, the water injection pipe 24 and the water extraction pipe 25 can be blocked when they are stationary, preventing dust from entering the interior of the filter when filtering or shutting down, and being filled into the filter frame 12 during cleaning, affecting the filtering work, while increasing the self-cleaning ability and maintaining good cleanliness;

[0071] The shovel block 21 descends, driving the cleaning rod 3 and the motor 31 to descend. The motor 31 drives the cleaning rod 3 to rotate. The cleaning rod 3 rotates to clean the surface of the filter cloth 13. The bottom of the shovel block 21 approaches the filter cloth 13 and peels off the filter cake. The cleaning rod 3 contacts the filter cloth 13 and sweeps the residual part to the bottom of the filter cloth 13 through the cleaning brush 32. The rubber ring 28 contacts the filter cloth 13 again to further scrape off the residue on the filter cloth 13. Through the continuous action of the above three effects, the filter cake is cleaned step by step according to the size of the volume, which distributes the cleaning pressure, improves the cleaning efficiency, reduces the cleaning time, and improves the filtration efficiency, thereby improving the extraction and purification efficiency of boson.

[0072] After each filter cloth 13 is cleaned, the shovel block 21 rises to lift the sealing block 26 until the cleaning groove 27 is aligned with the water injection pipe 24. The water injection pipe 24 flushes the cleaning rod and cleaning brush 32 in the cleaning groove 27 to improve the cleanliness of the cleaning rod 3 and the cleaning brush 32 and avoid contamination of the subsequent filter cloth 13;

[0073] The rotation of the cleaning rod 3 drives the cleaning plate 33 to rotate. When the cleaning plate 33 rotates to the top of the cleaning rod 3, it is squeezed and tilted by the inner wall of the cleaning groove 27. Then, the cleaning plate 33 rotates over the inner wall of the top of the cleaning groove 27. The cleaning plate 33 swings back from the tilted state due to the torsion spring. During the swinging and resetting process, the cleaning plate 33 touches the filter cloth 13, and the filter cloth 13 is vibrated by knocking, which loosens the impurities blocked in the filter cloth 13 and cooperates with the sweeping action of the cleaning brush 32 to improve the cleaning performance of the filter cloth 13. The cleaning plate 33 does not contact the cleaning brush 32 during the swinging process, so as to avoid the cleaning brush 32 blocking the swinging effect of the cleaning plate 33.

[0074] Example 3:

[0075] On the basis of the second embodiment, the bottom of the shovel block 21 is slidably connected to a swing rod 34 through a spring, and one end of the swing rod 34 is rotatably connected to a roller 35. The outer ring of the roller 35 contacts the filter cloth 13. When the cleaning plate 33 rotates until the bottom of the cleaning rod 3 is away from the filter cloth 13, it contacts and moves the swing rod 34.

[0076] The outer ring of the roller 35 is evenly provided with protrusions 36, and the surface of the protrusions 36 away from the roller 35 is evenly provided with rubber strips 37, which are arranged horizontally;

[0077] Specific working process: When the shovel block 21 peels off the filter cake, due to the strong adhesion between the filter cake and the filter cloth 13, if the agglomeration area of ​​the filter cake is small, when the small piece of filter cake is peeled off, the filter cloth 13 withstands a small force generated by the peeling. If the agglomeration area of ​​the filter cake is large, when the large piece of filter cake is peeled off, it exceeds the strength that the filter cloth 13 can withstand, which may cause the filter cloth 13 to tear; especially when the filter cloth 13 has been used for a long time and its strength has decreased, it is more likely to tear; therefore, when the shovel block 21 descends and contacts Before filtering the cake, the roller 35 first contacts the surface of the filter cake. As the shovel block 21 descends, the roller 35 rolls on the surface of the filter cake. Since protrusions 36 are evenly arranged on the outer ring of the roller 35, the roller 35 drives the protrusions 36 to rotate when rolling. For example, when a pentagon rolls on a straight line, the protrusions 36 collide and hit the filter cake multiple times during the rotation of the roller 35, breaking up large pieces of filter cake. The shovel block 21 then peels the broken filter cake off the filter cloth 13, thereby improving the cleanliness of the filter cloth 13 and improving the protection of the filter cloth 13.

[0078] By providing the rubber strip 37, the rubber strip 37 can increase the friction between the protrusion 36 and the filter cake surface based on the anti-slip properties of rubber, thereby promoting the rolling of the roller 35 and the protrusion 36 on the filter cake surface;

[0079] The cam 35 is pressed against the filter cloth 13 and the filter cloth 13 is then pulled back to the bottom of the filter cloth 13 so that the filter cloth 13 can be easily removed.

[0080] Example 4:

[0081] On the basis of the third embodiment, a storage rod 38 is installed on the filter body 1, and the water pipes connected to the water supply unit 23 on the water injection pipe 24 and the water extraction pipe 25 are wound around the storage rod 38; when the water pipes are in operation, they are retracted in the storage rod 38 near the water supply unit 23. When the water pipes are cleaned or stationary, the water pipes are inclined downward toward the water supply unit 23;

[0082] The water pipes for connecting the water injection pipe 24 and the water extraction pipe 25 are wound with a coil spring 39;

[0083] One end of the shovel block 21 is provided with a marking device 4, and the marking device 4 contacts the side edge of the filter frame 12, and the marking device 4 is used to mark the edge of the filter frame 12 where the filter cloth 13 is located;

[0084] Specific working process: the receiving rod 38 is provided on the filter body 1, and the bracket 2 does not contact it. When the slider 17 is stationary at one end of the guide rail, the water pipe is neatly wound around the receiving rod 38 by the contraction of the coil spring 39, so as to prevent the water pipe from being scattered everywhere and affecting the movement of the slider 17 or the bracket 2, or being pinched and damaged. Moreover, since the receiving rod 38 is arranged at an angle, the water injection pipe 24 is connected to the water extraction pipe 25 for water injection and cleaning. At this time, the horizontal position of the water extraction pipe 25 is high, so that during cleaning or stationary period, the water pipe is inclined downward toward the water supply unit 23, which helps to flush away impurities in the water extraction pipe 25 and the water pipe along the inclined posture with the water flow, thereby improving the self-cleaning effect, avoiding the influence of impurity accumulation, and facilitating the workers to clean the plate and frame filter, thereby improving the convenience of use.

[0085] Furthermore, a coil spring 39 is provided on the water pipe. The coil spring 39 is a conventional elastic component wound around the water pipe. On the one hand, it plays a role of stretching and contracting. On the other hand, it plays a supporting role at the curved part of the water pipe, thereby preventing the water pipe from being excessively bent and causing blockage, ensuring the smooth flow of the water pipe and maintaining normal operation.

[0086] The marking device 4 is in direct contact with the side edge of the filter frame 12 and adopts a coloring method, such as a roller brush or sponge with washable ink, or a labeling method such as an automatic labeling device, to ensure that the marking is clear and corresponds precisely to the position of the filter cloth; when the shovel block 21 moves, if an abnormal situation such as blockage or damage is detected by the above method, the marking device 4 is started, such as the coloring method: a roller brush or sponge is dipped in a washable paint, such as food-grade ink, to avoid contaminating the filter cloth, and short lines and other marks are painted on the edge of the filter frame 12 to identify the filter cloth status and distinguish blockage or damage; the mark generated by the marking device 4 directly corresponds to the abnormal part of the filter cloth 13 in the filter frame 12, which is convenient for workers to quickly identify the specific part of the filter cloth 13. Combined with subsequent careful inspection, the marking device 4 can make special symbols on the edge of the filter frame 12 corresponding to the problem filter cloth 13, providing intuitive guidance for workers to repair or replace the filter cloth 13, improving convenience and reducing the impact of the Bose-induced extraction process.

[0087] Embodiment 5:

[0088] A method for extracting and purifying bosine, as shown in the accompanying drawings of the specification Figure 1 As shown, the extraction and purification method comprises:

[0089] S1: The raw material is crushed and defatted, and after degreasing, the raw material is washed with clean water to remove residual solvent and water-soluble impurities, and then dried by drying equipment until the water content is less than 10%; the cell structure of the raw material is destroyed by rapid release of high-pressure steam, and deionized water is added according to a preset ratio of raw material to water, including 1:10-1:20, and stirred to form a substrate suspension;

[0090] S2: adding specific enzymes, including cellulase, to the suspension to produce an enzymatic hydrolyzate through enzymatic hydrolysis; the initially obtained enzymatic hydrolyzate is heated to inactivate the enzyme, filtered through a filter and adsorbed on activated carbon to remove impurities, and then concentrated under reduced pressure and adjusted in pH to obtain an enzymatic hydrolyzate for extraction;

[0091] S3: The enzymatic hydrolysate is subjected to resin adsorption, using a macroporous adsorption resin, which is pretreated and then loaded onto a chromatographic column. The concentrated enzymatic hydrolysate is loaded onto a sample at a predetermined flow rate, impurities are eluted with deionized water, and then phosphene is eluted with a 30%-50% ethanol solution, and the eluate is collected.

[0092] S4: Add activated carbon to the eluate, stir at room temperature, and filter to remove trace pigments; remove residual inorganic salts through a cation exchange resin and anion exchange resin in series to obtain a refined solution; concentrate the refined solution under reduced pressure at below 40°C to a solid content of 10%-20%; dry the concentrate in a spray dryer to a white powder to obtain bosin powder;

[0093] Specifically, this purification process uses a combined strategy of physical separation-adsorption purification-precision separation to ensure the high purity and biological activity of bosera while achieving a balance between process efficiency, cost control and environmental protection. It is particularly suitable for the large-scale preparation of functional small molecule active ingredients from plant enzymatic hydrolysates.

[0094] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the foregoing embodiments. The foregoing embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for purifying bosine from an enzymatic hydrolyzate, characterized in that: The purification method comprises: S1: The raw material is crushed and defatted, and after degreasing, the raw material is washed with clean water to remove residual solvent and water-soluble impurities, and then dried by drying equipment until the water content is less than 10%; the cell structure of the raw material is destroyed by rapid release of high-pressure steam, and deionized water is added according to a preset ratio of raw material to water, including 1:10-1:20, and stirred to form a substrate suspension; S2: adding specific enzymes, including cellulase, to the suspension to produce an enzymatic hydrolyzate through enzymatic hydrolysis; the initially obtained enzymatic hydrolyzate is heated to inactivate the enzyme, filtered through a filter and adsorbed on activated carbon to remove impurities, and then concentrated under reduced pressure and adjusted in pH to obtain an enzymatic hydrolyzate for extraction; S3: The enzymatic hydrolysate is subjected to resin adsorption, using a macroporous adsorption resin, which is pretreated and then loaded onto a chromatographic column. The concentrated enzymatic hydrolysate is loaded onto a sample at a predetermined flow rate, impurities are eluted with deionized water, and then phosphene is eluted with a 30%-50% ethanol solution, and the eluate is collected. S4: Add activated carbon to the eluate, stir at room temperature, and filter to remove trace pigments; remove residual inorganic salts through a cation exchange resin and anion exchange resin in series to obtain a refined liquid; concentrate the refined liquid under reduced pressure below 40°C to a solid content of 10%-20%; dry the concentrated liquid into a white powder in a spray drying tower to obtain bosphorin powder.

2. A device for purifying boson from an enzymatic hydrolyzate, comprising a filter, which comprises a filter body (1), a pressing device (11), a filter frame (12), a filter cloth (13), a feeding device (14) and a discharging device (15); characterized in that: The filter also includes: An electric guide rail (16) is installed on both sides of the filter body (1), and a slider (17) is slidably connected to the electric guide rail (16). A dial plate (18) is hinged on the top of the slider (17) through a torsion spring, and a sensor (19) is provided on one side of the dial plate (18) that contacts the surface of the filter frame (12); a bracket (2) is installed on the slider (17), and shovel blocks (21) are slidably connected on both sides of the bracket (2), and the bottom of the shovel blocks (21) is tilted toward the filter cloth (13), and one side of the two shovel blocks (21) contacts the surface of the filter cloth (13) on both sides of the filter frame (12), and the shovel blocks (21) are connected to the electric push rod (22) on the bracket (2); A water supply unit (23) is installed at one end of the filter body (1). A water injection pipe (24) is connected to the bracket (2) through a spring sliding connection. The water injection pipe (24) is connected to the water nozzle on the filter frame (12). Both sides of the water injection pipe (24) are in contact with the shovel block (21). A water extraction pipe (25) is provided in the shovel block (21). The water extraction pipe (25) contacts the filter cloth (13). The water supply unit (23) supplies water to the water injection pipe (24) and extracts water to the water extraction pipe (25).

3. The device for purifying boson from an enzymatic solution according to claim 2, characterized in that: The water injection pipe (24) is a four-way tube. The top of the water injection pipe (24) is connected to the water supply unit (23) through a water pipe. The bottom of the water injection pipe (24) is provided with a valve and is connected to the inside of the filter frame (12). The bracket (2) is located on both sides of the water injection pipe (24) and is connected to sealing blocks (26) through spring sliding. The bottom of the sealing block (26) contacts the shovel block (21); when the shovel block (21) rises to lift the sealing block (26), the water injection pipe (24) is connected to the water extraction pipe (25). When the shovel block (21) descends and drives the sealing block (26) to descend and recover, the water injection pipe (24) is away from the water extraction pipe (25), and the openings on both sides of the water injection pipe (24) are blocked by the sealing blocks (26).

4. The device for purifying boson from an enzymatic hydrolyzate according to claim 3, characterized in that: A cleaning groove (27) is provided on one side of the shovel block (21) close to the filter cloth (13), and a water pumping pipe (25) is located above the cleaning groove (27). The water pumping pipe (25) contacts the filter cloth (13) through a rubber ring (28) provided at the opening. A cleaning rod (3) is rotatably connected in the cleaning groove (27), and the cleaning rod (3) is connected to a motor (31) provided in the shovel block (21). A cleaning brush (32) is provided on the cleaning rod (3).

5. The device for purifying boson from an enzymatic hydrolyzate according to claim 4, characterized in that: A cleaning plate (33) is distributed in an annular manner on the cleaning rod (3). The cleaning plate (33) is hinged to the surface of the cleaning rod (3) through a torsion spring. When the cleaning plate (33) rotates to the top of the cleaning rod (3), it is squeezed and moved by the inner wall of the cleaning groove (27) to tilt.

6. The device for purifying boson from an enzymatic hydrolyzate according to claim 5, characterized in that: The bottom of the shovel block (21) is slidably connected to a swing rod (34) via a spring, one end of the swing rod (34) is rotatably connected to a roller (35), the outer ring of the roller (35) contacts the filter cloth (13), and when the cleaning plate (33) rotates until the bottom of the cleaning rod (3) is away from the filter cloth (13), it contacts and moves the swing rod (34).

7. The device for purifying boson from an enzymatic hydrolyzate according to claim 6, characterized in that: The outer ring of the roller (35) is evenly provided with protrusions (36), and the surface of the protrusions (36) away from the roller (35) is evenly provided with rubber strips (37), and the rubber strips (37) are arranged horizontally.

8. The device for purifying boson from an enzymatic hydrolyzate according to claim 3, characterized in that: A storage rod (38) is installed on the filter body (1), and the water pipes on the water injection pipe (24) and the water extraction pipe (25) connected to the water supply unit (23) are wound around the storage rod (38); when the water pipes are working, they are retracted to a position close to the water supply unit (23) on the storage rod (38); when the water pipes are cleaned or left stationary, the water pipes are inclined downward toward the water supply unit (23).

9. The device for purifying boson from an enzymatic hydrolyzate according to claim 8, characterized in that: A coil spring (39) is wound around the water pipes for connecting the water injection pipe (24) and the water extraction pipe (25).

10. The device for purifying boson from an enzymatic hydrolyzate according to claim 2, characterized in that: One end of the shovel block (21) is provided with a marking device (4), and the marking device (4) contacts the side edge of the filter frame (12). The marking device (4) is used to mark the edge of the filter frame (12) where the filter cloth (13) is located.

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