Mineral drinking water as well as preparation method and application thereof

Abstract

The invention discloses mineral drinking water as well as a preparation method and application thereof, and belongs to the technical field of drinking water, the mineral drinking water comprises the following raw materials in percentage by weight: 4-6% of fresh bamboo juice, 1.1-1.7% of dunaliella extract, 0.05-0.1% of coral bacteria extract and the balance of natural mineral water. A preparation method of the dunaliella salina extract comprises the following steps: washing dunaliella salina, and putting the dunaliella salina into a crusher for crushing treatment; mixing the dunaliella salina powder with 75-83% ethanol water solution, performing microwave-ultrasonic combined treatment, and centrifuging to obtain an extracting solution and filter residues; adding 0.6-1.2% of a sodium carbonate aqueous solution into the filter residue, extracting, centrifuging, mixing the supernate with the extracting solution, and carrying out reflux extraction; performing rotary evaporation concentration on the reflux extracting solution; purifying and eluting the concentrated solution; and carrying out flocculation treatment on the eluent by using a composite flocculant to obtain the dunaliella extract. The mineral drinking water prepared by the invention is easy to control the content of raw materials, has certain effects of reducing uric acid and improving renal function, and has a good long-term drinking effect.

Description

Technical Field

[0001] The present invention relates to the technical field of drinking water, and in particular to mineral drinking water and a preparation method and application thereof. Background Art

[0002] As people's health awareness continues to improve, the quality requirements for drinking water are also getting higher and higher. Traditional drinking water is mostly only simply purified and its ingredients are relatively simple, which cannot meet people's diverse needs for health and nutrition.

[0003] Chinese invention patent CN116350657A discloses a formula for a mineral crystal for regulating uric acid and a method for making the same. The formula has the effect of regulating uric acid and includes the following components: red cloud stone, longjiang stone and lapis lazuli. The uric acid balancing solution soaked in the mineral crystals can be taken by patients in the form of drinking water.

[0004] However, due to the poor solubility of red cloud stone, Longjiang stone and celestite, it is difficult to accurately control the mineral element content. At the same time, red cloud stone, Longjiang stone and celestite may also contain many other impurity elements. These impurity elements may also enter the drinking water and the content is difficult to control, thereby affecting the quality of drinking water. There are health risks in long-term drinking.

[0005] Based on this, the present invention designs a mineral drinking water and a preparation method and application thereof to solve the above problems. Summary of the Invention

[0006] In view of the above-mentioned shortcomings of the prior art, the present invention provides a mineral drinking water and a preparation method and application thereof.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: The invention discloses mineral drinking water, which comprises the following raw materials in percentage by weight: 4-6% fresh bamboo sap, 1.1-1.7% Dunaliella extract, 0.05-0.1% Xanthoceras extract, and the balance being natural mineral water.

[0008] Furthermore, the preparation method of the Dunaliella extract is: Step S1: washing the Dunaliella salina and then putting it into a grinder for grinding, and sieving it through 60-100 mesh to obtain Dunaliella salina powder; Step S2: mixing Dunaliella salina powder with a 75-83% ethanol aqueous solution, performing a microwave-ultrasound combined treatment at a temperature of 55-84° C., a microwave treatment power of 500-800 W, and a microwave treatment time of 4-10 minutes; and an ultrasonic treatment power of 400-500 W, and an ultrasonic treatment time of 2-5 minutes; and centrifuging to obtain an extract and a filter residue; Step S3: adding a 0.6-1.2% sodium carbonate aqueous solution to the filter residue, extracting at 55-68° C. for 1-3 hours, centrifuging, mixing the supernatant with the extract, and performing reflux extraction 1-2 times, each extraction for 1.2-2 hours, to obtain a reflux extract; Step S4: Concentrating the reflux extract by rotary evaporation; Step S5: purifying and eluting the concentrate using D101 resin or NKA-9 resin, and collecting the eluate; Step S6: flocculating the eluate with a composite flocculant, where the amount of the flocculant is 0.6-1% of the eluate, to obtain a Dunaliella extract.

[0009] Furthermore, in step S2, microwave treatment and ultrasonic treatment are performed simultaneously; and the mass ratio of Dunaliella salina powder to ethanol aqueous solution is 2-5:1.

[0010] Furthermore, in step S5, the purification and elution steps are as follows: after adsorption, standing for 2 to 8 hours, first eluting 3 to 5 BV with water, then eluting 5 to 8 BV with 5 to 10% ethanol aqueous solution, and then eluting 8 to 12 BV with 75 to 90% ethanol aqueous solution; the mass ratio of the resin to the Dunaliella salina powder is 4 to 9:1.

[0011] Furthermore, in step S6, the composite flocculant is composed of chitosan and 101 juice clarifier in a mass ratio of 1:2-4.

[0012] Furthermore, the preparation method of the wood coral fungus extract is as follows: the wood coral fungus is vacuum freeze-dried and then crushed, 3 to 5 portions of the wood coral fungus powder are added to 10 to 20 mL of Tris-EDTA (TE) buffer (pH 8.0), placed in a water bath at 40 to 45°C for 15 to 20 minutes, then placed in a cell disruptor for disruption, and supernatant A is collected by centrifugation; the above method is used for extraction 2 to 3 times, and all supernatants A are combined; a 5 to 10% trichloroacetic acid solution is added to the supernatant A, the supernatant is placed in a water bath at 40 to 45°C for 40 to 50 minutes, and supernatant B is obtained by centrifugation; supernatant B is added to 3 to 4 times the volume of anhydrous ethanol, and the supernatant is allowed to stand at 10 to 15°C for 5 to 10 hours. The precipitate is obtained after centrifugation, and the wood coral fungus extract is obtained.

[0013] Furthermore, the fresh bamboo sap is prepared by a distillation method: fresh bamboo is cut into small segments of 2 to 3 cm, washed and crushed into bamboo powder, the bamboo powder is placed in a distillation device, and water 1 to 2 times the mass of the bamboo powder is added, heated and distilled at 95 to 100° C., and the distillate is collected.

[0014] In order to better achieve the purpose of the present invention, the present invention also provides a method for preparing the mineral drinking water, comprising the following steps: taking fresh bamboo sap, Dunaliella extract, Xanthoceras extract and natural mineral water, mixing them evenly and then sterilizing them.

[0015] In order to better achieve the purpose of the present invention, the present invention also provides a mineral drinking water prepared according to the preparation method.

[0016] In order to better achieve the purpose of the present invention, the present invention also provides a use of the mineral drinking water in preparing a product for reducing uric acid or improving renal function.

[0017] Compared with the prior art, the present invention has the following beneficial effects: the raw material content of the mineral drinking water prepared by the present invention is easy to control, and it has a certain effect on reducing uric acid and improving kidney function, and the long-term drinking effect is good. DETAILED DESCRIPTION

[0018] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0019] Example 1: A method for preparing mineral drinking water, comprising the following steps: Step (1), preparing Dunaliella extract, specifically comprising the following steps: Step S1: washing the Dunaliella salina and then putting it into a grinder for grinding, and sieving it through a 60-mesh sieve to obtain Dunaliella salina powder; Step S2: mixing Dunaliella salina powder with a 75% ethanol aqueous solution at a mass ratio of Dunaliella salina powder to ethanol aqueous solution of 5:1, and subjecting the mixture to a combined microwave-ultrasonic treatment at a temperature of 55° C., a microwave treatment power of 800 W, and a microwave treatment time of 4 minutes; and an ultrasonic treatment power of 500 W and an ultrasonic treatment time of 2 minutes; centrifuging to obtain an extract and a filter residue; the microwave treatment and ultrasonic treatment being performed simultaneously; Step S3: adding 0.6% sodium carbonate aqueous solution to the filter residue, wherein the mass ratio of the filter residue to the sodium carbonate aqueous solution is 1:20; extracting at 55° C. for 3 h, centrifuging, mixing the supernatant with the extract, and performing reflux extraction once, each extraction for 2 h, to obtain a reflux extract; Step S4: Concentrating the reflux extract by rotary evaporation; Step S5: The concentrate was purified and eluted using D101 resin, with the mass ratio of resin to Dunaliella salina powder being 4:1; the purification and elution steps were as follows: after adsorption, the concentrate was allowed to stand for 8 hours, eluted with water for 3 BV, then eluted with 10% ethanol aqueous solution for 5 BV, and then eluted with 90% ethanol aqueous solution for 8 BV, and the eluate was collected; Step S6: flocculating the eluate with a composite flocculant, wherein the composite flocculant is composed of chitosan and 101% juice clarifier in a mass ratio of 1:2. The amount of the flocculant is 1% of the eluate, thereby obtaining a Dunaliella extract.

[0020] Step (2), preparing the extract of the wood coral fungus: the wood coral fungus is vacuum freeze-dried and then crushed, 3 g of the wood coral fungus powder is added to 20 mL of Tris-EDTA (TE) buffer (pH 8.0), placed in a water bath at 40°C for 20 minutes, and then placed in a cell disruptor for disruption, the cell disruptor parameters are: 80 Hz, disruption for 6 minutes; centrifugation to collect supernatant A; the above method is used for extraction twice, and all supernatants A are combined; a 10% trichloroacetic acid solution is added to the supernatant A, the supernatant is placed in a water bath at 40°C for 50 minutes, and the supernatant B is obtained by centrifugation; the supernatant B is added to 3 times the volume of anhydrous ethanol, and the supernatant is allowed to stand at 15°C for 5 hours. The precipitate is obtained after centrifugation, and the wood coral fungus extract is obtained.

[0021] Step (3), preparing fresh bamboo sap: chop fresh bamboo into 2 cm small segments, wash and crush into bamboo powder, put the bamboo powder into a distillation device, add water twice the weight of the bamboo powder, heat and distill at 95 ° C, and collect the distillate.

[0022] Step (4): weigh the following raw materials: 4% fresh bamboo sap, 1.7% Dunaliella extract, 0.05% Xanthoceras extract, and the balance natural mineral water; mix well and sterilize.

[0023] Example 2: A method for preparing mineral drinking water, comprising the following steps: Step (1), preparing Dunaliella extract, specifically comprising the following steps: Step S1: washing the Dunaliella salina and then putting it into a grinder for grinding, and sieving it through 100 mesh to obtain Dunaliella salina powder; Step S2: mixing Dunaliella salina powder with 83% ethanol aqueous solution, wherein the mass ratio of Dunaliella salina powder to ethanol aqueous solution is 2:1, and performing microwave-ultrasonic combined treatment at a temperature of 84° C., a microwave treatment power of 500 W, and a microwave treatment time of 10 minutes; and an ultrasonic treatment power of 400 W and a ultrasonic treatment time of 5 minutes; centrifuging to obtain an extract and a filter residue; the microwave treatment and ultrasonic treatment are performed simultaneously; Step S3: adding a 1.2% sodium carbonate aqueous solution to the filter residue, wherein the mass ratio of the filter residue to the sodium carbonate aqueous solution is 1:10; extracting at 68° C. for 1 hour, centrifuging, mixing the supernatant with the extract, and performing reflux extraction once, each extraction for 1.2 hours, to obtain a reflux extract; Step S4: Concentrating the reflux extract by rotary evaporation; Step S5: The concentrate was purified and eluted using NKA-9 resin, with the mass ratio of resin to Dunaliella salina powder being 9:1. The purification and elution steps were as follows: after adsorption, the concentrate was allowed to stand for 2 hours, eluted with water for 5 BV, then with 5% aqueous ethanol for 8 BV, and then with 75% aqueous ethanol for 12 BV, and the eluate was collected; Step S6: flocculating the eluate with a composite flocculant comprising chitosan and 101% juice clarifier in a mass ratio of 1:4. The amount of the flocculant used is 0.6% of the eluate, thereby obtaining a Dunaliella extract.

[0024] Step (2), preparing the extract of the wood coral fungus: the wood coral fungus is vacuum freeze-dried and then crushed, 5 g of the wood coral fungus powder is added to 10 mL of Tris-EDTA (TE) buffer (pH 8.0), placed in a water bath at 45°C for 15 minutes, and then placed in a cell disruptor for disruption, the cell disruptor parameters are: 100 Hz, disruption for 4 minutes; centrifugation to collect supernatant A; the above method is used for extraction 3 times, and all supernatants A are combined; a 5% trichloroacetic acid solution is added to the supernatant A, the supernatant is placed in a water bath at 45°C for 40 minutes, and the supernatant B is obtained by centrifugation; the supernatant B is added to 4 times the volume of anhydrous ethanol, and the supernatant is allowed to stand at 10°C for 10 hours. The precipitate is obtained after centrifugation, and the wood coral fungus extract is obtained.

[0025] Step (3), preparing fresh bamboo sap: chop fresh bamboo into 3 cm small segments, wash and crush into bamboo powder, put the bamboo powder into a distillation device, add water 1 times the weight of the bamboo powder, heat and distill at 100 ° C, and collect the distillate.

[0026] Step (4): weigh the following raw materials: 6% fresh bamboo sap, 1.1% Dunaliella extract, 0.1% Xanthoceras extract, and the balance natural mineral water; mix well and sterilize.

[0027] Example 3: A method for preparing mineral drinking water, comprising the following steps: Step (1), preparing Dunaliella extract, specifically comprising the following steps: Step S1: washing the Dunaliella salina and then putting it into a grinder for grinding, and sieving it through 80 mesh to obtain Dunaliella salina powder; Step S2: mixing Dunaliella salina powder with a 78% ethanol aqueous solution at a mass ratio of Dunaliella salina powder to ethanol aqueous solution of 3.5:1, and subjecting the mixture to a microwave-ultrasonic combined treatment at a temperature of 70° C., a microwave treatment power of 700 W, and a microwave treatment time of 6 minutes; and an ultrasonic treatment power of 450 W and a ultrasonic treatment time of 3 minutes; centrifuging to obtain an extract and a filter residue; the microwave treatment and ultrasonic treatment being performed simultaneously; Step S3: adding a 1% sodium carbonate aqueous solution to the filter residue, wherein the mass ratio of the filter residue to the sodium carbonate aqueous solution is 1:13; extracting at 62° C. for 2.2 hours, centrifuging, mixing the supernatant with the extract, and performing reflux extraction twice, each extraction for 1.5 hours, to obtain a reflux extract; Step S4: Concentrating the reflux extract by rotary evaporation; Step S5: The concentrate was purified and eluted using D101 resin, with the mass ratio of resin to Dunaliella salina powder being 5:1. The purification and elution steps were as follows: after adsorption, the concentrate was allowed to stand for 4 hours, eluted with water for 4 BV, then eluted with 7% ethanol aqueous solution for 7 BV, and then eluted with 82% ethanol aqueous solution for 10 BV, and the eluate was collected; Step S6: flocculating the eluate with a composite flocculant, wherein the composite flocculant is composed of chitosan and 101 juice clarifier in a mass ratio of 1:3. The amount of the flocculant is 0.7% of the eluate, thereby obtaining a Dunaliella extract.

[0028] Step (2), preparing the extract of the wood coral fungus: the wood coral fungus is vacuum freeze-dried and then crushed, 4 g of the wood coral fungus powder is added to 15 mL of Tris-EDTA (TE) buffer (pH 8.0), placed in a water bath at 42°C for 17 minutes, and then placed in a cell disruptor for disruption, the cell disruptor parameters are: 90 Hz, disruption for 5 minutes; centrifugation to collect supernatant A; the above method is used for extraction twice, and all supernatants A are combined; a 6% trichloroacetic acid solution is added to the supernatant A, the supernatant is placed in a water bath at 43°C for 45 minutes, and the supernatant B is obtained by centrifugation; the supernatant B is added to 3.5 times the volume of anhydrous ethanol, and the supernatant is allowed to stand at 12°C for 7 hours. The precipitate is obtained after centrifugation, and the wood coral fungus extract is obtained.

[0029] Step (3), preparing fresh bamboo sap: chop fresh bamboo into 2.5 cm small segments, wash and crush into bamboo powder, put the bamboo powder into a distillation device, add water 1.5 times the weight of the bamboo powder, heat and distill at 98°C, and collect the distillate.

[0030] Step (4): weigh the following raw materials: 5% fresh bamboo sap, 1.4% Dunaliella extract, 0.08% Xanthoceras extract, and the balance natural mineral water; mix well and sterilize.

[0031] Comparative Example 1: The difference from Example 3 is that the Dunaliella extract is not contained.

[0032] Comparative Example 2: The difference from Example 3 is that the extract of the fungus Ficus microcarpa is not contained.

[0033] Comparative Example 3: The difference from Example 3 is that the Dunaliella extract and the Xanthoceras extract are not contained.

[0034] Experimental Example: To evaluate the efficacy of drinking water in lowering uric acid, urea nitrogen, and creatinine levels in mice, the method is as follows: Mouse husbandry: 100 4-week-old male CD-1 mice, weighing 24-32 g, were housed at 25°C, 55% relative humidity, and a 12-h light-dark cycle for one week with free access to food and water. Ten mice were randomly selected from the 100 mice as a blank control group, receiving 1.0 mL / kg of distilled water by gavage.

[0035] Construction of a hyperuricemia mouse model: Another 90 mice were gavaged with 1.0 mL / kg of 2.5% sodium carboxymethylcellulose suspension for one week. On the eighth day, 100 mg / kg of adenine combined with 300 mg / kg of potassium oxonate were gavaged to obtain a hyperuricemia mouse model.

[0036] Hyperuric acid mouse model grouping: 80 mice were randomly selected from 90 hyperuric acid mice and divided into 8 groups, with 10 mice in each group, namely, hyperuric acid model group, drug group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group.

[0037] One week after modeling, groups 1, 2, and 3, as well as groups 1, 2, and 3, were gavaged with the corresponding drinking water at 20 mL / kg / day; the blank control group and the hyperuricemia model group were given an equal volume of normal saline. The drug group was gavaged with allopurinol tablets at a dose of 33.3 mg / kg for 12 consecutive weeks (3 months). After the experiment, the mice's uric acid, urea nitrogen, and creatinine levels were measured (average values ​​were taken for each group). The results are shown in Table 1.

[0038] Table 1 Effects of drinking water on uric acid, urea nitrogen, and creatinine levels in hyperuricemia mice

[0039] As can be seen, compared to the hyperuricemia model group, creatinine, uric acid, and urea nitrogen levels in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, and Comparative Example 3 all showed some reduction in creatinine, uric acid, and urea nitrogen levels, with Example 3 showing the most significant effect. Example 1, Example 2, and Example 3 demonstrated a certain effect in lowering uric acid and improving renal function, with long-term drinking effects approaching those of the medication group. Comparative Example 1, Comparative Example 2, and Comparative Example 3 demonstrate that the Dunaliella extract and the Xanthoceras extract have a synergistic effect in lowering uric acid and improving renal function.

[0040] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A mineral drinking water, characterized in that: The invention comprises the following raw materials in percentage by weight: 4-6% fresh bamboo sap, 1.1-1.7% Dunaliella extract, 0.05-0.1% of Xanthoceras edulis extract, and the balance is natural mineral water.

2. The mineral drinking water according to claim 1, characterized in that The preparation method of the Dunaliella extract is as follows: Step S1: washing the Dunaliella salina and then putting it into a grinder for grinding, and sieving it through 60-100 mesh to obtain Dunaliella salina powder; Step S2: mixing Dunaliella salina powder with a 75-83% ethanol aqueous solution, performing a microwave-ultrasound combined treatment at a temperature of 55-84° C., a microwave treatment power of 500-800 W, and a microwave treatment time of 4-10 minutes; and an ultrasonic treatment power of 400-500 W, and an ultrasonic treatment time of 2-5 minutes; and centrifuging to obtain an extract and a filter residue; Step S3: adding a 0.6-1.2% sodium carbonate aqueous solution to the filter residue, extracting at 55-68° C. for 1-3 hours, centrifuging, mixing the supernatant with the extract, and performing reflux extraction 1-2 times, each extraction for 1.2-2 hours, to obtain a reflux extract; Step S4: Concentrating the reflux extract by rotary evaporation; Step S5: purifying and eluting the concentrate using D101 resin or NKA-9 resin, and collecting the eluate; Step S6: flocculating the eluate with a composite flocculant, where the amount of the flocculant is 0.6-1% of the eluate, to obtain a Dunaliella extract.

3. The mineral drinking water according to claim 2, characterized in that In step S2, microwave treatment and ultrasonic treatment are performed simultaneously; the mass ratio of Dunaliella salina powder to ethanol aqueous solution is 2-5:

1.

4. The mineral drinking water according to claim 3, characterized in that In step S5, the purification and elution steps are as follows: after adsorption, standing for 2 to 8 hours, first eluting 3 to 5 BV with water, then eluting 5 to 8 BV with 5 to 10% ethanol aqueous solution, and then eluting 8 to 12 BV with 75 to 90% ethanol aqueous solution; the mass ratio of the resin to the Dunaliella salina powder is 4 to 9:

1.

5. The mineral drinking water according to claim 4, characterized in that In step S6, the composite flocculant is composed of chitosan and 101 juice clarifier in a mass ratio of 1:2-4.

6. The mineral drinking water according to claim 5, characterized in that The preparation method of the wood coral fungus extract is as follows: the wood coral fungus is vacuum freeze-dried and then crushed, 3 to 5 portions of the wood coral fungus powder are added to 10 to 20 mL of Tris-EDTA buffer, placed in a water bath at 40 to 45° C. for 15 to 20 minutes, then placed in a cell disruptor for disruption, and supernatant A is collected by centrifugation; the above method is used for extraction 2 to 3 times, and all supernatants A are combined; a 5 to 10% trichloroacetic acid solution is added to the supernatant A, the supernatant is placed in a water bath at 40 to 45° C. for 40 to 50 minutes, and supernatant B is collected by centrifugation; supernatant B is added to 3 to 4 times the volume of anhydrous ethanol, and the supernatant is allowed to stand at 10 to 15° C. for 5 to 10 hours. The precipitate is obtained after centrifugation, and the wood coral fungus extract is obtained.

7. The mineral drinking water according to claim 6, characterized in that The fresh bamboo sap is prepared by a distillation method: fresh bamboo is cut into small segments of 2 to 3 cm, washed and crushed into bamboo powder, the bamboo powder is placed in a distillation device, and water 1 to 2 times the weight of the bamboo powder is added, heated and distilled at 95 to 100° C., and the distillate is collected.

8. A method for preparing mineral drinking water according to any one of claims 1 to 7, characterized in that: The following steps are involved: Take fresh bamboo sap, Dunaliella extract, Xanthoceras extract and natural mineral water, mix them evenly and then sterilize them.

9. Mineral drinking water prepared according to the preparation method of claim 8.

10. Use of the mineral drinking water according to any one of claims 1 to 7 in the preparation of a product for reducing uric acid or improving renal function.

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