Physical detection and analysis equipment based on stem cell characteristics

Abstract

The invention relates to the technical field of cell detection, and particularly discloses a physical detection and analysis device based on stem cell characteristics, the physical detection and analysis device comprises a detection mechanism arranged in an analyzer, the detection mechanism comprises mounting plates, the mounting plates are arranged in an annular array, and the ends, close to each other, of the mounting plates are fixedly connected; according to the stem cell detection liquid preparation device, a test tube is installed in an analyzer through the detection mechanism, so that preparation of the stem cell detection liquid can be completed, and compared with a traditional stem cell detection liquid preparation process, the stem cell detection liquid preparation device has the advantages that the detection efficiency is greatly improved; the test tube needs to be opened for multiple times to extract supernate or add a buffer solution and the like, the operation is simple, the detection and analysis efficiency of the stem cells is improved, stem cell suspension and the like cannot be exposed in an external environment, the damage of the stem cells is reduced, the supernate is conveniently and accurately extracted, and the influence on subsequent detection and analysis use is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of cell detection, and in particular to a physical detection and analysis device based on stem cell characteristics. Background Art

[0002] Testing the physical properties of stem cells is typically performed to ensure the quality, stability, and safety of stem cell preparations in clinical applications. This testing can help prevent adverse reactions caused by excessively high or low osmotic pressure. During stem cell testing and analysis, the stem cell suspension undergoes multiple centrifugation and washing steps to create a stem cell testing solution for stem cell testing.

[0003] Publication number CN120275236B discloses a device and method for testing finished stem cell drugs. The background technology raises the following issues: existing stem cell analysis equipment is difficult to achieve a fully automated closed-loop process; during the centrifugation and washing processes, the lid needs to be constantly opened and closed to remove and add liquids, reducing detection efficiency.

[0004] Based on the existing technology, the following problems exist: During the detection and analysis of stem cells, the test tube needs to be constantly opened and closed to extract the supernatant, etc., which reduces work efficiency. The above application documents only make the test tube vertical or inclined during the opening and closing process to improve the centrifugal effect and facilitate the extraction of the supernatant, etc. However, it still requires opening and closing the test tube plug multiple times, which reduces the detection efficiency and exposes the stem cell suspension to the external environment, causing damage to the stem cells, which is not conducive to actual detection and analysis. There are certain shortcomings. In order to solve the above problems, a physical detection and analysis device based on stem cell characteristics is proposed. Summary of the Invention

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: a device for physical detection and analysis of stem cell characteristics, comprising an analyzer and a sampling mechanism, and also comprising a detection mechanism disposed within the analyzer, the detection mechanism comprising: The mounting slot is located inside the analyzer. A mounting plate is rotatably mounted inside the mounting slot. The mounting plates are arranged in a circular array and are fixedly connected at their respective ends. A detection component for preparing the stem cell detection solution is located on the top of each mounting plate. A positioning component is located outside the mounting slot. The detection component includes: A spline shaft is slidably arranged at one end of the mounting plate away from each other, and the spline shaft is capable of self-rotation, a connecting piece is fixedly provided on the top of the spline shaft, a mounting shell for mounting a test tube is fixedly provided on the top of the connecting piece, and a distance measuring sensor is fixedly provided on the side wall of the connecting piece; The first toothed disc is fixedly sleeved on the side wall of the spline shaft and located at the bottom of the mounting plate. The second toothed disc is fixedly sleeved on the side wall of the spline shaft and located at the bottom of the first toothed disc.

[0006] Furthermore, the detection component further includes: The bearing seat and the fixed sleeve are arranged at one end of the mounting plate away from each other. The inner wall of the bearing seat is provided with a bearing. The outer ring of the bearing is fixedly connected to the inner wall of the bearing seat. The inner ring fixed sleeve of the bearing is provided with a spline sleeve. The inner wall of the spline sleeve is engaged with the side wall of the spline shaft, so that the spline shaft can move along the axial direction of the spline sleeve and the spline shaft can rotate.

[0007] Furthermore, the detection component further includes: A rotating member is provided on the side wall of the spline shaft and is located at the bottom of the bearing seat; The adjustment plate is sleeved on the top of the mounting plate and is L-shaped. The horizontal end of the adjustment plate is rotatably connected to the side wall of the spline shaft through a rotating member, and the vertical end of the adjustment plate is provided with spaced jacks.

[0008] Furthermore, the detection component further includes: The adjusting seat is fixed on the top of the mounting plate, and an adjusting groove is provided on the side wall of the adjusting seat. The inner wall of the adjusting groove is specially designed, and a plug rod is sleeved on the inner wall of the adjusting groove. The side wall of the plug rod is fixedly sleeved with a plug ring, and the side walls of the plug ring and the plug rod are respectively fitted with the special-shaped inner wall of the adjusting groove. The two ends of the plug rod extend to both sides of the adjusting seat respectively, and the side walls of the plug rod are adapted to the inner wall of the socket. A spring is sleeved on the side wall of one end of the plug rod located in the adjusting groove and on the side of the plug ring away from the socket.

[0009] Furthermore, the detection component further includes: A rubber tube is fixedly sleeved on the inner wall of the mounting shell, and the rubber tube and the side wall of the mounting shell are both provided with openings. The outer wall of the test tube is provided with a first scale line and a second scale line arranged at intervals, and the first scale line and the second scale line are staggered, and the first scale line and the second scale line are oriented toward the opening of the mounting shell; The card block is fixed on the bottom inner wall of the installation shell and extends into the rubber tube. The bottom of the test tube is provided with a card slot adapted to the card block.

[0010] Furthermore, the detection component further includes: An inner bevel is provided on the inner wall of the test tube so as to make the inner wall of the test tube inclined; A first retaining ring is fixedly sleeved on the inner wall of the test tube, a shielding plane is provided on the bottom of the first retaining ring, and an inclined surface is provided on the inner wall of the first retaining ring; The second retaining ring is fixedly sleeved on the inner wall of the test tube and is located on the top of the first retaining ring.

[0011] Furthermore, the detection mechanism further includes: A first servo motor is fixedly mounted inside the analyzer, wherein the output shaft of the first servo motor is fixedly mounted with a first rotating shaft via a coupling, and the first rotating shaft is fixedly connected to the bottom of one end of the mounting plate close to each other to drive the mounting plate to rotate; An inner gear ring is fixedly mounted on the inner side wall of the mounting groove and is adapted to the first gear plate and the second gear plate, so as to adjust the speed of the spline shaft rotation by meshing with the first gear plate or the second gear plate; The eccentric ring is fixedly arranged on the outer side of the mounting groove to determine the position of the detection component.

[0012] Furthermore, the positioning component includes: The outer gear ring is rotatably arranged on the outside of the eccentric ring, the top of the outer gear ring is fixedly provided with a first rod body, and the upper end of the side wall of the first rod body is fixedly provided with a camera; The second rod body is fixedly arranged at the lower end of the side wall of the first rod body and is designed in a bent shape. An identification block is fixedly arranged on the outer side of the lower end of the second rod body.

[0013] Furthermore, the positioning component further includes: A second servo motor is fixedly arranged inside the analyzer, wherein the output shaft of the second servo motor is fixedly provided with a second rotating shaft via a coupling, a gear is fixedly provided on a side wall of the second rotating shaft, and the gear is meshed with the outer gear ring; The annular slider is fixed on the bottom of the outer gear ring. An annular groove is provided inside the analyzer and outside the mounting groove. The annular slider is placed in the annular groove to limit the rotation of the outer gear ring.

[0014] Furthermore, the sampling mechanism includes: The first electric slide is fixed inside the analyzer, the sliding end of the first electric slide is fixed with the second electric slide, the sliding end of the second electric slide is fixed with the third electric slide, and the sliding end of the third electric slide is fixed with a sampling tube.

[0015] The present invention provides a device for physical detection and analysis of stem cell characteristics. Compared with existing technologies, it has the following advantages: 1. The present invention uses a detection mechanism to install the test tube in the back of the analyzer to complete the preparation of the stem cell detection solution. Compared with the traditional process of preparing the stem cell detection solution, which requires opening the test tube multiple times to extract the supernatant or add buffer, the operation is simple, the efficiency of stem cell detection and analysis is improved, and the stem cell suspension is not exposed to the external environment, thereby reducing damage to the stem cells. It also facilitates the accurate extraction of the supernatant and avoids affecting the subsequent detection and analysis.

[0016] 2. The present invention utilizes the characteristic that liquid adheres closely to the inner wall of the container under the action of centrifugal force to restrict the stem cell suspension, etc. in the test tube by providing a first baffle ring and a shielding plane, thereby reducing the probability of the stem cell suspension, etc. splashing out of the test tube during centrifugation. The second baffle ring and the inclined surface further block and drain the stem cell suspension, thereby preventing leakage of the stem cell suspension during centrifugation. There is no need to press the plug at the opening of the test tube to restrict leakage of the stem cell suspension, etc. in the test tube, which facilitates operation and facilitates sampling by the sampling mechanism, thereby improving detection efficiency.

[0017] 3. The present invention utilizes the characteristic that the distance from the center point of the eccentric ring to the inner wall is different. By monitoring the distance from the center point to the inner wall of the eccentric ring through a distance measuring sensor, the position and orientation of the test tube can be determined, thereby facilitating the identification of the boundary between the supernatant and the sediment, and facilitating the sampling mechanism to extract a specified amount of supernatant. By staggering the first scale line and the second scale line, the accuracy of camera acquisition is avoided from being affected by the scale blocking the boundary between the supernatant and the sediment, and it is convenient to mark the scale at multiple positions of the test tube, avoiding the accuracy of the scale marking being affected by the tilt of the inner wall of the test tube.

[0018] 4. The present invention uses the detection assembly to drive the test tube to revolve along the output shaft of the first servo motor while causing the first gear disc or the second gear disc to rotate, thereby causing the test tube to rotate. Compared with the traditional centrifugation operation that only rotates the test tube around the first servo motor, i.e., revolves, the centrifugation effect is better. By adjusting the height of the adjustment plate, the meshing of the first or second toothed disc with the inner gear ring is adjusted. By making the transmission ratio between the first or second toothed disc and the inner gear ring different, the rotation speed of the test tube during the revolution is adjusted, thereby facilitating adjustment of the centrifugal effect according to the specifications of the stem cell suspension, etc., facilitating the configuration of stem cell detection solutions of different specifications in multiple test tubes, thereby facilitating subsequent detection and analysis of different stem cell detection solutions, and improving the efficiency of detection and analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of a longitudinal cross-sectional structure of the analyzer of the present invention; Figure 3 It is a structural schematic diagram of the detection mechanism of the present invention; Figure 4 This is a schematic diagram of the detection assembly and inner gear ring structure of the present invention; Figure 5 It is a schematic diagram of the positioning assembly and eccentric ring structure of the present invention; Figure 6 It is a schematic diagram of the longitudinal cross-sectional structure of the eccentric ring, annular slider and outer gear ring of the present invention; Figure 7 This is a schematic structural diagram of the identification block, the second rod body and the camera of the present invention; Figure 8 Schematic diagram of the detection assembly, camera and eccentric ring structure of the present invention; Figure 9 This is a schematic structural diagram of the inner gear ring, mounting plate and detection assembly of the present invention; Figure 10 It is a longitudinal cross-sectional structural diagram of the bearing seat, mounting plate, bearing and spline sleeve of the present invention; Figure 11 It is a schematic diagram of the exploded structure of the mounting plate, spline shaft, spline sleeve, bearing, bearing seat, rotating member, adjustment plate, first gear disc and second gear disc of the present invention; Figure 12 It is a schematic diagram of the longitudinal cross-sectional structure of the mounting plate, the adjustment seat and the adjustment plate of the present invention; Figure 13 This is a schematic diagram of the longitudinal cross-sectional structure of the mounting housing, the connecting piece and the rubber tube of the present invention; Figure 14 This is a schematic diagram of the longitudinal cross-sectional structure of a test tube of the present invention; Figure 15 It is a schematic structural diagram of the sampling component of the present invention.

[0020] Reference numerals in the above drawings: 1. analyzer; 2. sampling mechanism; 3. detection mechanism; 21. Third electric slide rail; 22. Sampling tube; 23. Second electric slide rail; 24. First electric slide rail; 31. Mounting slot; 32. Eccentric ring; 33. Positioning assembly; 34. First servo motor; 35. Mounting plate; 36. Internal gear ring; 37. Detection assembly; 331, second servo motor; 332, first rod; 333, outer ring gear; 334, second rod; 335, camera; 336, gear; 337, annular slider; 338, identification block; 370. First gear disc; 371. First scale mark; 372. Test tube; 3721. Block; 3722. Second retaining ring; 3723. First retaining ring; 3724. Inner bevel; 3725. Blocking plane; 3726. Inclined surface; 373. Mounting shell; 374. Rubber tube; 375. Second scale mark; 376. Connector; 377. Distance measuring sensor; 378. Spline shaft; 379. Second gear disc; 3790. Socket; 3791. Spline sleeve; 3792. Bearing; 3793. Bearing seat; 3794. Rotating part; 3795. Adjustment plate; 3796. Insert rod; 3797. Adjustment seat; 3798. Insert ring; 3799. Adjustment slot. DETAILED DESCRIPTION

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] Example 1: Please refer to Figure 1 、 Figure 2 and Figure 15 A device for physical detection and analysis of stem cell characteristics includes an analyzer 1 and a sampling mechanism 2, wherein the sampling mechanism 2 includes: The first electric slide 24 is fixed inside the analyzer 1 , the sliding end of the first electric slide 24 is fixed with the second electric slide 23 , the sliding end of the second electric slide 23 is fixed with the third electric slide 21 , and the sliding end of the third electric slide 21 is fixed with the sampling tube 22 .

[0023] In specific implementation, the position of the sampling tube 22 is adjusted by the first electric slide 24, the second electric slide 23 and the third electric slide 21, so that the sampling tube 22 can be inserted into any test tube 372, thereby facilitating the extraction of the supernatant and the like.

[0024] The sampling tube 22 is connected to an external pipeline and is extracted and discharged through a pump body. By making the external pipeline a multi-pipe design, the sampling tube 22 can transport the supernatant or stem cell detection liquid to different locations under the action of the pump body, and can add buffer solution to the test tube 372. The connection of the external pipeline is an existing technology and will not be described in detail here.

[0025] See also Figure 2 、 Figure 3 、 Figure 4 、 Figure 9 、 Figure 10 、 Figure 11 、 Figure 12 and Figure 13 , further comprising a detection mechanism 3 disposed inside the analyzer 1, the detection mechanism 3 comprising: The mounting slot 31 is provided inside the analyzer 1. A mounting plate 35 is rotatably mounted inside the mounting slot 31. The mounting plates 35 are arranged in a circular array and fixedly connected at their respective ends. A detection assembly 37 for preparing a stem cell detection solution is disposed on the top of each mounting plate 35. A positioning assembly 33 is disposed outside the mounting slot 31. The detection assembly 37 includes: A spline shaft 378 is slidably mounted on one end of the mounting plate 35 away from each other and is capable of self-rotation. A connector 376 is fixedly mounted on the top of the spline shaft 378. A mounting housing 373 for mounting a test tube 372 is fixedly mounted on the top of the connector 376. A distance sensor 377 is fixedly mounted on the side wall of the connector 376. The first gear disc 370 is fixedly mounted on the side wall of the spline shaft 378 and located at the bottom of the mounting plate 35 . The second gear disc 379 is fixedly mounted on the side wall of the spline shaft 378 and located at the bottom of the first gear disc 370 .

[0026] The detection component 37 also includes: The bearing seat 3793 is fixedly sleeved on one end of the mounting plate 35 away from each other. The inner wall of the bearing seat 3793 is provided with a bearing 3792. The outer ring of the bearing 3792 is fixedly connected to the inner wall of the bearing seat 3793. The inner ring of the bearing 3792 is fixedly sleeved with a spline sleeve 3791. The inner wall of the spline sleeve 3791 is engaged with the side wall of the spline shaft 378, so that the spline shaft 378 can move along the axial direction of the spline sleeve 3791 and the spline shaft 378 can rotate.

[0027] The detection component 37 also includes: The rotating member 3794 is provided on the side wall of the spline shaft 378 and is located at the bottom of the bearing seat 3793; The adjustment plate 3795 is mounted on the top of the mounting plate 35 and is L-shaped. The horizontal end of the adjustment plate 3795 is rotatably connected to the side wall of the spline shaft 378 through a rotating member 3794. The vertical end of the adjustment plate 3795 is provided with spaced-apart sockets 3790.

[0028] The detection component 37 also includes: The adjusting seat 3797 is fixed on the top of the mounting plate 35. The side wall of the adjusting seat 3797 is provided with an adjusting groove 3799. The inner wall of the adjusting groove 3799 is of a special-shaped design. The inner wall of the adjusting groove 3799 is sleeved with an insert rod 3796. The side wall of the insert rod 3796 is fixedly sleeved with an insert ring 3798. The insert ring 3798 and the side walls of the insert rod 3796 are respectively fitted with the special-shaped inner walls of the adjusting groove 3799. The two ends of the insert rod 3796 extend to both sides of the adjusting seat 3797 respectively. The side walls of the insert rod 3796 are adapted to the inner wall of the socket 3790. A spring is sleeved on the side wall of one end of the insert rod 3796 located in the adjusting groove 3799 and on the side of the insert ring 3798 away from the socket 3790.

[0029] The detection component 37 also includes: A rubber tube 374 is fixedly mounted on the inner wall of the mounting shell 373. Both the rubber tube 374 and the sidewall of the mounting shell 373 have openings. The outer wall of the test tube 372 is provided with first and second scale lines 371 and 375 arranged at intervals. The first and second scale lines 371 and 375 are staggered and face the opening of the mounting shell 373. The clamping block 3721 is fixedly mounted on the bottom inner wall of the mounting shell 373 and extends into the rubber tube 374 . The bottom of the test tube 372 is provided with a clamping groove adapted to the clamping block 3721 .

[0030] In a specific implementation, a test tube 372 containing a stem cell suspension and a serum culture medium is placed in a rubber tube 374, and the test tube 372 is tightened by the rubber tube 374 so that the test tube 372 is stably installed in the mounting shell 373. After the test tube 372 is installed, the first servo motor 34 is started. The first servo motor 34 drives the mounting plate 35 to rotate through the first rotating shaft, thereby driving the spline shaft 378, the connecting piece 376 and the mounting shell 373 to rotate, thereby driving the test tube 372 to rotate along the output shaft of the first servo motor 34. In this process, since the first gear disc 370 or the second gear disc 379 is engaged with the inner gear ring 36, when the first gear disc 370 or the second gear disc 379 rotates along the output shaft of the first servo motor 34, the first gear disc 370 or the second gear disc 379 rotates, thereby causing the test tube 372 to rotate. Compared with the traditional centrifugal operation of only rotating the test tube 372 around the first servo motor 34, that is, revolving, the centrifugal effect is better.

[0031] By inserting the rod 3796 into the socket 3790 at different positions, the height of the adjustment plate 3795 is adjusted, and then the height of the spline shaft 378 is adjusted to adjust the engagement of the first gear disc 370 or the second gear disc 379 with the inner gear ring 36. By making the transmission ratio of the first gear disc 370 or the second gear disc 379 to the inner gear ring 36 different, the rotation speed of the test tube 372 during the revolution is adjusted, so as to facilitate the adjustment of the centrifugal effect according to the specifications of the stem cell suspension, and facilitate the configuration of different specifications of stem cell detection liquid in multiple test tubes 372, so as to facilitate the subsequent detection and analysis of different stem cell detection liquids and improve the efficiency of the detection and analysis. In the specific operation, the rod 3796 is pulled to make the rod 3796 and the socket engage. 3790 is disengaged, and then the height of the adjustment plate 3795 is adjusted. The adjustment plate 3795 drives the spline shaft 378, the first gear plate 370 and the second gear plate 379 to move vertically through the rotating member 3794, thereby adjusting the first gear plate 370 or the second gear plate 379 to engage with the inner gear ring 36. After the adjustment is completed, the insertion rod 3796 is inserted into the socket 3790 at the corresponding position, and the insertion rod 3796 is released. Under the action of the spring, the insertion rod 3796 is stably inserted into the socket 3790, thereby stably adjusting the first gear plate 370 or the second gear plate 379 to engage with the inner gear ring 36. By rotatably connecting the adjustment plate 3795 to the spline shaft 378, the height of the spline shaft 378 is easily adjusted without affecting the rotation of the spline shaft 378.

[0032] A spacing is left between the first toothed disc 370 and the second toothed disc 379 to ensure that the first toothed disc 370 or the second toothed disc 379 can engage with the inner gear ring 36 individually to avoid affecting actual transmission use. The number of the first toothed disc 370 and the second toothed disc 379, as well as the transmission ratio of the first toothed disc 370, the second toothed disc 379 and the inner gear ring 36 can be adjusted according to actual needs and will not be elaborated here.

[0033] The special-shaped inner wall of the adjustment groove 3799 is specifically as follows: the inner wall diameters of the adjustment groove 3799 are different, so as to respectively fit with the side walls of the insert ring 3798 and the insert rod 3796, so that the insert ring 3798 cooperates with the stopper of the adjustment groove 3799 to limit the insertion rod 3796 from being separated from the slot, and facilitates the spring to elastically support the insertion rod 3796, thereby improving the stability of the insertion rod 3796 in limiting the adjustment plate 3795.

[0034] The spline shaft 378 can move vertically in the spline sleeve 3791, and the mounting plate 35 is rotationally connected to the spline sleeve 3791 through the bearing seat 3793 and the bearing 3792, so that the spline shaft 378 can move vertically and rotate.

[0035] By staggering the first scale line 371 and the second scale line 375, that is, there is a difference between the numerical values ​​corresponding to the scales of the first scale line 371 and the second scale line 375, for example, if the first scale line 371 shows 7mm, the second scale line 375 is limited to 5mm, that is, there is a difference of 2mm between the first scale line 371 and the second scale line 375. Taking this as an example, the scales of the first scale line 371 and the second scale line 375 are staggered, thereby avoiding affecting the accuracy of the camera 335's acquisition due to the scale blocking the boundary between the supernatant and the sediment, and the first scale line 371 and the second scale line 375 facilitate the scale marking of multiple positions of the test tube 372, avoiding affecting the accuracy of the scale marking due to the tilt of the inner wall of the test tube 372.

[0036] The first rotating shaft is rotationally connected to the analyzer 1 to ensure the stability of the rotation of the first rotating shaft.

[0037] The analyzer 1 is internally equipped with temperature control devices such as a heater and a temperature sensor to control the temperature of the stem cell suspension, etc. This is a prior art and is not shown in the figure and will not be described in detail here.

[0038] See also Figure 14 , the detection component 37 also includes: An inner slope 3724 is formed on the inner wall of the test tube 372 so that the inner wall of the test tube 372 is inclined; A first retaining ring 3723 is fixedly mounted on the inner wall of the test tube 372. A shielding plane 3725 is provided at the bottom of the first retaining ring 3723, and an inclined surface 3726 is provided on the inner wall of the first retaining ring 3723. The second retaining ring 3722 is fixedly mounted on the inner wall of the test tube 372 and is located on the top of the first retaining ring 3723 .

[0039] During centrifugation, the stem cell suspension within the test tube 372 clings to the inner wall of the test tube 372 due to centrifugal force. The first retaining ring 3723 and the shielding plane 3725 follow the movement trajectory of the stem cell suspension, thereby blocking it. This restricts the stem cell suspension within the test tube 372 and reduces the probability of the stem cell suspension splashing out of the test tube 372 during centrifugation. Some of the stem cell suspension that splashes along the inner wall of the first retaining ring 3723 enters between the first retaining ring 3723 and the second retaining ring 3722, where it is further blocked by the second retaining ring 3722. Furthermore, the inclined surface 3726 guides the stem cell suspension to the bottom of the first retaining ring 3723, thereby preventing leakage of the stem cell suspension during centrifugation. This eliminates the need for conventional pressure on the plunger at the opening of the test tube 372 to restrict leakage of the stem cell suspension within the test tube 372. This facilitates operation and facilitates sampling by the sampling mechanism 2, thereby improving detection efficiency.

[0040] During the centrifugation process, the centrifugal movement needs to be started and stopped slowly to avoid causing the stem cell suspension in the test tube 372 to be agitated and splash out of the test tube 372 .

[0041] By setting the inner bevel 3724, the inner wall of the test tube 372 is designed to be inclined. Compared with the traditional method of tilting the test tube 372 during the centrifugation process, the test tube 372 is reset to a vertical shape after the centrifugation is completed, thereby improving the centrifugal effect. At the same time, the test tube 372 is always in a vertical shape, which is convenient for actual sampling. By setting the inclination angle of the inner bevel 3724, it does not exceed the center line of the test tube 372, thereby not affecting the actual sampling use of the sampling tube 22.

[0042] During the installation of the test tube 372, the test tube 372 is squeezed by the rubber tube 374 to improve the installation stability of the test tube 372, and the position of the test tube 372 is limited by embedding the card block 3721 into the card slot, so that the first scale line 371 and the second scale line 375 are oriented toward the opening of the installation shell 373 and the rubber tube 374, so that the boundary between the supernatant and the sediment of the stem cell suspension after centrifugation can be observed through the camera 335, thereby facilitating the sampling mechanism 2 to take out the supernatant. In addition, due to the provision of the inner bevel 3724, the bottom of the test tube 372 has space for opening the card slot.

[0043] See also Figure 2 、 Figure 3 、 Figure 9 and Figure 10 , the detection mechanism 3 also includes: A first servo motor 34 is fixedly mounted inside the analyzer 1. The output shaft of the first servo motor 34 is fixedly mounted with a first rotating shaft via a coupling. The first rotating shaft is fixedly connected to the bottom of one end of the mounting plate 35 that is close to each other, so as to drive the mounting plate 35 to rotate. The first servo motor 34 drives the mounting plate 35 to rotate, thereby driving the test tube 372 to rotate. The inner gear ring 36 is fixedly mounted on the inner side wall of the mounting groove 31 and is adapted to the first gear plate 370 and the second gear plate 379 so as to adjust the rotation speed of the spline shaft 378 by meshing with the first gear plate 370 or the second gear plate 379; The eccentric ring 32 is fixedly arranged on the outside of the mounting groove 31 to determine the position of the detection component 37. Due to the different distances from the center point of the eccentric ring 32 to the inner wall, when the centrifugation is completed, the distance from the center point of the eccentric ring 32 to the inner wall is monitored by the distance measuring sensor 377 to determine the position of the test tube 372, and the first scale line 371 and the second scale line 375 are directed to the inner wall of the eccentric ring 32 to cooperate with the identification block 338 of the positioning component 33, so as to facilitate the grasp of the position of the test tube 372, and the boundary between the supernatant and the sediment is facilitated by the camera 335, so that the sampling mechanism 2 can extract a specified amount of supernatant.

[0044] Example 2: Please refer to Figure 5 、 Figure 6 、 Figure 7 and Figure 8 The technical solution of this embodiment is different from that of the first embodiment in that the positioning component 33 includes: The outer gear ring 333 is rotatably mounted on the outside of the eccentric ring 32. A first rod 332 is fixedly mounted on the top of the outer gear ring 333. A camera 335 is fixedly mounted on the upper end of the side wall of the first rod 332. The second rod 334 is fixed to the lower end of the side wall of the first rod 332 and is designed to be bent. An identification block 338 is fixed to the outer side of the lower end of the second rod 334 .

[0045] The positioning component 33 also includes: A second servo motor 331 is fixedly mounted inside the analyzer 1. The output shaft of the second servo motor 331 is fixedly mounted with a second rotating shaft via a coupling. A gear 336 is fixedly mounted on the side wall of the second rotating shaft. The gear 336 is meshed with the outer gear ring 333. The annular slider 337 is fixed to the bottom of the outer gear ring 333. An annular groove is provided inside the analyzer 1 and outside the mounting groove 31. The annular slider 337 is placed in the annular groove to limit the rotation of the outer gear ring 333.

[0046] During specific implementation, after the position of the test tube 372 is grasped by the eccentric ring 32 and the distance sensor 377, the second servo motor 331 drives the gear 336 to rotate, and the gear 336 drives the outer ring gear 333 to rotate under the limit of the annular slider 337 and the annular slide groove, thereby adjusting the position of the camera 335 so that the camera 335 can capture the boundary between the supernatant and the sediment, which is convenient for the sampling mechanism 2 to take samples.

[0047] When the camera 335 approaches the target test tube 372 , the distance to the identification block 338 is measured by the distance measuring sensor 377 , thereby further confirming the position of the test tube 372 and improving accuracy.

[0048] The first servo motor 34 and the second servo motor 331 of the present invention are connected to the controller and the external power supply through wires to facilitate actual control and use. This is existing technology and will not be described in detail here.

[0049] During the implementation of the present invention, a stem cell suspension is placed in a test tube 372 and thawed. After thawing, a serum culture medium is added. Then, the door of the analyzer 1 is opened, and the thawed stem cell suspension is placed in the mounting shell 373. The first scale line 371 and the second scale line 375 are directed toward the opening of the mounting shell 373 by limiting the block 3721 and the slot. Then, the door is closed, and the first servo motor 34 is started to drive the detection components 37 arranged in a circular array to perform a circular motion along the output shaft of the first servo motor 34, thereby causing the test tube 372 to perform a circular motion along the output shaft of the first servo motor 34. At the same time, the test tube 372 is caused to rotate by itself through the cooperation of the inner gear ring 36, the first gear disc 370 and the second gear disc 379. Compared with the transmission that can only be achieved through The test tube 372 is caused to revolve along the output shaft of the first servo motor to improve the centrifugal effect of the stem cell suspension in the test tube 372. After centrifugation is completed, the distance sensor 377 cooperates with the eccentric ring 32 to determine the position of the test tube 372 and the orientation of the first test tube 372 and the second test tube 372. By cooperating with the positioning component 33, the boundary position between the supernatant and the sediment is easily determined, thereby cooperating with the sampling mechanism 2 to facilitate the accurate removal of the supernatant. After the supernatant is removed, a buffer solution is added to the test tube 372 through the sampling mechanism 2, and the supernatant and sediment are obtained by the above-mentioned centrifugation method to wash the stem cells. After multiple washes, a stem cell detector is obtained, and the stem cell detection liquid is transported to the analysis position through the sampling mechanism 2 for detection and analysis of the stem cells.

[0050] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0051] It should be noted that, in this document, relational terms such as first and second, etc. are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations.

[0052] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and alterations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A physical detection and analysis device based on stem cell characteristics, comprising an analyzer and a sampling mechanism, characterized in that: The analyzer further includes a detection mechanism disposed inside the analyzer, the detection mechanism including: The mounting slot is located inside the analyzer. A mounting plate is rotatably mounted inside the mounting slot. The mounting plates are arranged in a circular array and are fixedly connected at their respective ends. A detection component for preparing the stem cell detection solution is located on the top of each mounting plate. A positioning component is located outside the mounting slot. The detection component includes: A spline shaft is slidably arranged at one end of the mounting plate away from each other, and the spline shaft is capable of self-rotation, a connecting piece is fixedly provided on the top of the spline shaft, a mounting shell for mounting a test tube is fixedly provided on the top of the connecting piece, and a distance measuring sensor is fixedly provided on the side wall of the connecting piece; The first toothed disc is fixedly sleeved on the side wall of the spline shaft and located at the bottom of the mounting plate. The second toothed disc is fixedly sleeved on the side wall of the spline shaft and located at the bottom of the first toothed disc.

2. The physical detection and analysis device based on stem cell characteristics according to claim 1, characterized in that: The detection component also includes: The bearing seat and the fixed sleeve are arranged at one end of the mounting plate away from each other. The inner wall of the bearing seat is provided with a bearing. The outer ring of the bearing is fixedly connected to the inner wall of the bearing seat. The inner ring fixed sleeve of the bearing is provided with a spline sleeve. The inner wall of the spline sleeve is engaged with the side wall of the spline shaft, so that the spline shaft can move along the axial direction of the spline sleeve and the spline shaft can rotate.

3. The physical detection and analysis device based on stem cell characteristics according to claim 1, characterized in that: The detection component also includes: A rotating member is provided on the side wall of the spline shaft and is located at the bottom of the bearing seat; The adjustment plate is sleeved on the top of the mounting plate and is L-shaped. The horizontal end of the adjustment plate is rotatably connected to the side wall of the spline shaft through a rotating member, and the vertical end of the adjustment plate is provided with spaced jacks.

4. The physical detection and analysis device based on stem cell characteristics according to claim 3, characterized in that: The detection component also includes: The adjusting seat is fixed on the top of the mounting plate, and an adjusting groove is provided on the side wall of the adjusting seat. The inner wall of the adjusting groove is specially designed, and a plug rod is sleeved on the inner wall of the adjusting groove. The side wall of the plug rod is fixedly sleeved with a plug ring, and the side walls of the plug ring and the plug rod are respectively fitted with the special-shaped inner wall of the adjusting groove. The two ends of the plug rod extend to both sides of the adjusting seat respectively, and the side walls of the plug rod are adapted to the inner wall of the socket. A spring is sleeved on the side wall of one end of the plug rod located in the adjusting groove and on the side of the plug ring away from the socket.

5. The physical detection and analysis device based on stem cell characteristics according to claim 1, characterized in that: The detection component also includes: A rubber tube is fixedly sleeved on the inner wall of the mounting shell, and the rubber tube and the side wall of the mounting shell are both provided with openings. The outer wall of the test tube is provided with a first scale line and a second scale line arranged at intervals, and the first scale line and the second scale line are staggered, and the first scale line and the second scale line are oriented toward the opening of the mounting shell; The card block is fixed on the bottom inner wall of the installation shell and extends into the rubber tube. The bottom of the test tube is provided with a card slot adapted to the card block.

6. The physical detection and analysis device based on stem cell characteristics according to claim 1, characterized in that: The detection component also includes: An inner bevel is provided on the inner wall of the test tube so as to make the inner wall of the test tube inclined; A first retaining ring is fixedly sleeved on the inner wall of the test tube, a shielding plane is provided on the bottom of the first retaining ring, and an inclined surface is provided on the inner wall of the first retaining ring; The second retaining ring is fixedly sleeved on the inner wall of the test tube and is located on the top of the first retaining ring.

7. The physical detection and analysis device based on stem cell characteristics according to claim 1, characterized in that: The detection mechanism also includes: A first servo motor is fixedly mounted inside the analyzer, wherein the output shaft of the first servo motor is fixedly mounted with a first rotating shaft via a coupling, and the first rotating shaft is fixedly connected to the bottom of one end of the mounting plate close to each other to drive the mounting plate to rotate; An inner gear ring is fixedly mounted on the inner side wall of the mounting groove and is adapted to the first gear plate and the second gear plate, so as to adjust the speed of the spline shaft rotation by meshing with the first gear plate or the second gear plate; The eccentric ring is fixedly arranged on the outer side of the mounting groove to determine the position of the detection component.

8. The physical detection and analysis device based on stem cell characteristics according to claim 7, characterized in that: The positioning component includes: The outer gear ring is rotatably arranged on the outside of the eccentric ring, the top of the outer gear ring is fixedly provided with a first rod body, and the upper end of the side wall of the first rod body is fixedly provided with a camera; The second rod body is fixedly arranged at the lower end of the side wall of the first rod body and is designed in a bent shape. An identification block is fixedly arranged on the outer side of the lower end of the second rod body.

9. The physical detection and analysis device based on stem cell characteristics according to claim 7, characterized in that: The positioning component also includes: A second servo motor is fixedly arranged inside the analyzer, wherein the output shaft of the second servo motor is fixedly provided with a second rotating shaft via a coupling, a gear is fixedly provided on a side wall of the second rotating shaft, and the gear is meshed with the outer gear ring; The annular slider is fixed on the bottom of the outer gear ring. An annular groove is provided inside the analyzer and outside the mounting groove. The annular slider is placed in the annular groove to limit the rotation of the outer gear ring.

10. The physical detection and analysis device based on stem cell characteristics according to claim 1, characterized in that: The sampling mechanism comprises: The first electric slide is fixed inside the analyzer, the sliding end of the first electric slide is fixed with the second electric slide, the sliding end of the second electric slide is fixed with the third electric slide, and the sliding end of the third electric slide is fixed with a sampling tube.

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