Ganoderma leukotrichum strain as well as related products and cultivation method thereof

Abstract

The invention discloses a Ganoderma leucoconitum strain and a related product and a cultivation method thereof, and relates to the field of microorganisms, and the Ganoderma leucoconitum strain is Ganoderma leucoconitum strain TGL10 which is preserved in the culture collection center of Guangdong Institute of Microbiology, is named as Ganoderma leucconitum in Latin taxonomy, and has a preservation number of GDMCC No: 66044 or a progeny of the Ganoderma leucoconitum strain TGL10. Ganoderma leukotrichum cultivated based on the strain has good plateau adaptability, excellent appearance and form and high yield, the average yield of each bag is 139.93 g (wet weight), the conversion rate is close to 27.99% and is higher than that of other strains at the present stage, and the high-quality (kidney type and nearly circular) accounts for 90% or above.

Description

Technical Field

[0001] The present invention relates to the field of microorganisms, in particular to a white-fleshed Ganoderma lucidum strain and related products and a cultivation method thereof. Background Art

[0002] White Ganoderma lucidum ( Ganoderma leucocontextum ) is a rare medicinal fungus. Its rich content of polysaccharides, triterpenoids, and other active ingredients have significant benefits in immune regulation, anti-tumor, liver protection, and detoxification, leading to growing market demand. However, existing cultivation techniques for white-fleshed Ganoderma lucidum still face the following challenges, hindering its large-scale promotion and high-quality production: (1) Low and unstable yield: Traditional cultivation methods rely heavily on the domestication of wild strains, resulting in slow mycelial growth and a biological efficiency (fresh Ganoderma yield / dry material weight) of only 40% to 60%, far lower than some high-yielding Ganoderma varieties (such as Ganoderma lucidum). Existing strains are susceptible to contamination by foreign bacteria in artificial environments, especially under the low temperature and strong ultraviolet conditions of plateau regions. Mycelial resistance is insufficient, resulting in a drop of more than 30% in Ganoderma yield. (2) Uneven quality: The triterpenoid content of commonly cultivated strains fluctuates greatly (0.5% to 1.2%), and the polysaccharide yield is only 1.5% to 2.0%, making it difficult to meet the raw material standards for high-end pharmaceuticals and health products. Some strains are prone to producing deformed mushrooms (such as thin caps and low spore production) in low-oxygen and high-altitude environments, which affects their commercial value; (3) Poor adaptability to plateaus: Plateau areas (2500-4000 meters above sea level) have large temperature differences between day and night (up to 20°C) and thin oxygen (oxygen partial pressure drops by 30%-40%). Conventional strains in this environment will have delayed budding, poor fruiting bodies, or may even be completely unable to produce mushrooms.

[0003] Existing technologies mostly use greenhouse heating or artificial oxygenation, which are costly and energy-intensive, making them difficult to promote in remote plateau areas.

[0004] In view of this, the present invention is proposed. Summary of the Invention

[0005] The purpose of the present invention is to provide a white-fleshed Ganoderma lucidum strain and related products and a cultivation method.

[0006] The present invention is achieved in that: In the first aspect, the present invention provides a white flesh Ganoderma lucidum strain, which is preserved in the strain collection center of Guangdong Institute of Microbiology and named Ganoderma leucocontextum , the white-fleshed Ganoderma lucidum strain TGL10 with the deposit number GDMCCNo: 66044 or its progeny.

[0007] In a second aspect, an embodiment of the present invention provides a bacterial agent containing the white-fleshed Ganoderma lucidum strain described in the aforementioned embodiment.

[0008] In a third aspect, an embodiment of the present invention provides a fermentation product containing the white-fleshed Ganoderma lucidum strain described in the aforementioned embodiment.

[0009] In a fourth aspect, an embodiment of the present invention provides a method for cultivating white-fleshed Ganoderma lucidum, comprising: cultivating the white-fleshed Ganoderma lucidum strain described in the above embodiment.

[0010] The present invention has the following beneficial effects: The present invention screened and obtained a new white flesh Ganoderma lucidum strain, which is preserved in the strain collection center of Guangdong Institute of Microbiology and named in Latin taxonomy as Ganoderma leucocontextum The white-fleshed Ganoderma lucidum strain TGL10, deposited with GDMCC No. 66044, or its progeny, has been selected. The white-fleshed Ganoderma lucidum cultivated from this strain exhibits good adaptability to plateau conditions, excellent appearance, and high yield, with an average yield of 139.93 grams per bag (wet weight) and a conversion rate of nearly 27.99%, exceeding that of other strains currently available. High-quality (kidney-shaped and nearly round) Ganoderma lucidum accounts for over 90%. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0012] Figure 1 This is a schematic diagram of the fruiting body of cultivated white-fleshed Ganoderma lucidum; Figure 2 This is a morphological diagram of the fruiting body of white-fleshed Ganoderma lucidum. DETAILED DESCRIPTION

[0013] To make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are described clearly and completely below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer are used. Where the manufacturer of the reagents or instruments is not specified, all are conventional products that can be purchased commercially.

[0014] Definition of noun In the context of two or more nucleic acid or polypeptide sequences, the terms "identical" or "percent identity" refer to two or more sequences or subsequences that are identical or have a specified percentage of identical amino acid residues or nucleotides when compared and aligned for maximum correspondence. Methods of sequence alignment for comparison are well known in the art. Once aligned, the number of matches is determined by counting the number of positions at which the identical nucleotide or amino acid residue occurs in the two sequences. The percent sequence identity is determined by dividing the number of matches by the length of the sequence as described in the identified sequence, or by the length of a join (such as 100 consecutive nucleotides or amino acid residues from the sequence as described in the identified sequence), and then multiplying the resulting value by 100.

[0015] Optimal alignment of sequences for comparison can be achieved, for example, by the local homology algorithm of Smith and Waterman, Adv. Appl. Math. 2:482, 1981; the homology alignment algorithm of Needleman and Wunsch, Mol. Biol. 48:443, 1970; the search for similarity method of Pearson and Lipman, Proc. Nat'l. Acad. Sci. USA 85:2444, 1988; computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics software package, Genetics Computer Group, 575 Science Dr., Madison, WI); or by manual alignment and visual inspection (see, for example, Sambrook et al. (Molecular Cloning: A Laboratory Manual, 4th Edition, Cold Spring Harbor, New York, NY). , 2012) and Ausubel et al. (In Current Protocols in Molecular Biology, John Wiley and Sons, New York, Suppl 104, 2013)).

[0016] The term "mushroom stick" is a common form of culture medium packaging in the cultivation of white-fleshed Ganoderma lucidum. It refers to the sterilized culture medium placed in a plastic bag, inoculated with fungi, and then cultured and germinated.

[0017] The term "primordium" refers to the initial stage of fruiting body development, which usually appears as a tiny tumor or dot-like structure formed by the differentiation of mycelium, which will develop into a complete fruiting body in the future.

[0018] The terms "first", "second", etc., if used, are merely used to distinguish and describe, and should not be understood as indicating or implying relative importance.

[0019] Technical Solution On the one hand, the embodiment of the present invention provides a white flesh Ganoderma lucidum strain, which is preserved in the strain collection center of Guangdong Institute of Microbiology and is named Ganoderma leucocontextum , or its progeny, the white-fleshed Ganoderma lucidum strain TGL10 with a deposit number of GDMCC No. 66044. The deposit date of the white-fleshed Ganoderma lucidum strain TGL10 is March 21, 2025, and the deposit address is Guangdong Microbiological Culture Collection Center, 5th Floor, Building 59, Compound 100, Xianlie Middle Road, Guangzhou, Institute of Microbiology, Guangdong Academy of Sciences.

[0020] In some embodiments, the nucleotide sequence identity between the genome sequence of the offspring and the genome sequence of the white-fleshed Ganoderma lucidum strain TGL10 is ≥80%.

[0021] In some embodiments, the at least 80% or more can be any one of 80%, 82%, 84%, 86%, 88%, 90%, 92%, 94%, 96%, 98% and 100%, or a range between any two of them.

[0022] The white-fleshed Ganoderma lucidum strain provided in the embodiment of the present invention is easy to activate and the culture method is simple to operate. There is no special limitation on the culture conditions, and the existing culture medium and culture conditions applied to the white-fleshed Ganoderma lucidum strain can be used.

[0023] On the other hand, an embodiment of the present invention provides a bacterial agent containing the white-fleshed Ganoderma lucidum strain described in any of the aforementioned embodiments.

[0024] On the other hand, an embodiment of the present invention provides a fermentation product containing the white-fleshed Ganoderma lucidum strain described in any of the aforementioned embodiments.

[0025] On the other hand, an embodiment of the present invention provides a method for cultivating white-fleshed Ganoderma lucidum, comprising: cultivating the white-fleshed Ganoderma lucidum strain described in any of the aforementioned embodiments.

[0026] In some embodiments, the cultivation method comprises the following steps: Inoculating the mother strain or original strain of the white-fleshed Ganoderma lucidum strain described in any of the preceding embodiments into a first culture medium for cultivation to obtain seeds for cultivation; inoculating the cultivation seeds into a cultivation bag containing a second culture matrix, and culturing until the mycelium is fully grown to obtain a mature cultivation bag; The mature cultivation bags are cultivated and managed to obtain fruiting bodies.

[0027] In some embodiments, the method for preparing the mother strain of the white-fleshed Ganoderma lucidum strain comprises: inoculating the white-fleshed Ganoderma lucidum strain into a PDA culture medium and culturing the strain.

[0028] In some embodiments, the first culture matrix includes the following components and the weight percentages of the components: 85% to 95% wheat, 1% to 10% bran, 1% to 5% gypsum powder and 1% to 10% quicklime powder, a water content of 50% to 70%, and a pH of 9 to 10.

[0029] In some embodiments, in the first culture matrix, the weight percentage of wheat can be any one of 85%, 86%, 88%, 90%, 92%, 94% and 95%, or a range between any two of them. The weight percentage of wheat bran can be any one of 1%, 2%, 4%, 6%, 8%, 10%, or a range between any two of them. The weight percentage of gypsum powder can be any one of 1%, 2%, 4%, 5%, or a range between any two of them. The weight percentage of quicklime powder can be any one of 1%, 2%, 4%, 6%, 8%, 10%, or a range between any two of them. The water content can be any one of 50%, 52%, 54%, 56%, 58%, 60%, 62%, 64%, 66%, 68%, 70%, or a range between any two of them, and the pH can be any one of 9, 9.2, 9.4, 9.6, 9.8, 10, or a range between any two of them.

[0030] In some embodiments, the second culture matrix includes the following components and the weight percentages of the components: 75% to 85% wood chips, 5% to 15% bran, 1% to 10% corn flour, 1% to 5% gypsum powder and 1% to 5% quicklime powder, with a moisture content of 50% to 70% and a pH of 8 to 9.

[0031] In some embodiments, in the second culture matrix, the weight percentage of wood chips can be any one of 75%, 76%, 78%, 80%, 82%, 84%, 85%, or a range between any two of them. The weight percentage of corn flour can be any one of 1%, 2%, 4%, 6%, 8%, 10%, or a range between any two of them. The weight percentage of gypsum powder can be any one of 1%, 2%, 4%, 5%, or a range between any two of them. The weight percentage of quicklime powder can be any one of 1%, 2%, 4%, 5%, or a range between any two of them. The water content can be any one of 50%, 52%, 54%, 56%, 58%, 60%, 62%, 64%, 66%, 68%, 70%, or a range between any two of them, and the pH can be any one of 8, 8.2, 8.4, 8.6, 8.8, or 9, or a range between any two of them.

[0032] In some embodiments, the wood cuttings include Cyclobalanopsis glauca wood cuttings.

[0033] In some embodiments, the cultivation bag cultivation conditions include: a temperature of 25-26°C, a humidity of 55%-60%, and a natural CO2 concentration. Specifically, the temperature can be any one of 25, 25.5, or 26°C, or a range between any two of them. The humidity can be any one of 55%, 56%, 57%, 58%, 59%, or 60%, or a range between any two of them.

[0034] In some embodiments, after the cultivation bag is cultured until it is covered with mycelia, the cultivation method further comprises: culturing the cultivation bag covered with mycelia for another 1 to 6 days to obtain the mature cultivation bag.

[0035] In some embodiments, the soil moisture for the cultivation is 65% to 70%, specifically any one of 65%, 66%, 67%, 68%, 69%, 70%, or a range between any two of them, and the moist layer is ≥ 40 cm, specifically any one of 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60 cm, or a range between any two of them.

[0036] In some embodiments, the cultivation beds are 4-6 cm deep and 1.3-1.7 m wide, and the width of the corridors between the two beds is 0.3-0.7 m. Specifically, the depth can be any one of 4, 5, or 6 cm, or a range between any two of them. The width can be any one of 1.3, 1.4, 1.5, 1.6, or 1.7 m, or a range between any two of them. The width of the corridors can be any one of 0.3, 0.4, 0.5, 0.6, or 0.7 m, or a range between any two of them.

[0037] In some embodiments, the cultivation steps include: removing the mature cultivation bags, placing the mushroom sticks vertically in the bed, covering with soil until the top of the mature cultivation bags is exposed; and watering 4 to 6 days after covering with soil.

[0038] In some embodiments, the exposed height of the top is 4 to 6 cm, specifically any one of 4, 5, and 6 cm, or a range between any two of them.

[0039] In some embodiments, the bag removal process includes: opening the cultivation bag or removing part of the plastic bag.

[0040] In some embodiments, the partial plastic bag includes 1 / 4 to 1 / 2 plastic bag, specifically 1 / 4, 1 / 3 or 1 / 2.

[0041] In some embodiments, the step of placing the mushroom sticks vertically in the bed includes: placing the mushroom sticks vertically in the bed with the opening or the removed part facing downward.

[0042] In some embodiments, the output management includes the following steps: After covering the soil and watering, open the exposed top of the mature cultivation bag. Do not water it for 6 to 8 days after the top is opened. Control the temperature to 20 to 25°C (for example, 20, 22, 24 or 25°C), the humidity to 45% to 55% (for example, 45%, 46%, 48%, 50%, 52%, 54% or 55%), and the light intensity to 2,000 to 5,000 lx (for example, 2000, 2500, 300, 3500, 4000, 4500 or 5000lx), with natural CO2 concentration, wait until the mycelium at the top opening recovers to the point where primordia appear, control the humidity at 70%-75% (for example, 70%, 71%, 72%, 73%, 74% or 75%), wait until the primordia grow to the target size and seal the top opening, control the temperature at 25-30°C (for example, 25, 26, 27, 28, 29 or 30°C), and the humidity at 80%-90% (for example, 80%, 82%, 84%, 86%, 88% or 90%), until Ganoderma lucidum buds appear and grows to the target size.

[0043] When Ganoderma lucidum buds and grows to the target size, control the temperature to 22-30℃ (for example, 20, 22, 24, 26, 28 or 30℃), water once every 2 to 3 days, mist spray for 10 to 15 minutes (for example, 10, 12, 14 or 15 minutes), maintain the humidity at 65% to 85% (for example, 65%, 66%, 68%, 70%, 72%, 74%, 76%, 78%, 80%, 82%, 84% or 85%), light exposure to natural light, ventilation for 0.5 to 2 hours (0.5, 1, 1.5 or 2 hours) every day, CO2 concentration at natural level, until the stipe grows to a length of 10 to 15 cm (for example, 10, 12, 14 or 15 cm).

[0044] When the stipe grows to 10-15 cm in length, control the temperature at night to 17-20°C (for example, 17, 18, 19 or 20°C) and at daytime to 25-30°C (for example, 25, 26, 27, 28, 29 or 30°C), increase ventilation and humidity until the cap grows to 8-12 cm (for example, 8, 9, 10, 11 or 12 cm).

[0045] In some embodiments, the increased ventilation includes: a ventilation time of 8 to 12 hours, specifically any one of 8, 9, 10, 11, and 12 hours, or a range between any two of them.

[0046] In some embodiments, the increased ventilation includes: daytime ventilation.

[0047] In some embodiments, increasing the humidity includes controlling the humidity to 60%-85% and spraying water 1-3 times per day, each time for 10-15 minutes. Specifically, the humidity is controlled to be within the range of 60%, 65%, 70%, 75%, 80%, and 85%, or any two thereof; the number of sprayings can be 1, 2, or 3 times, and the duration of each spray can be within the range of 10, 12, 14, or 15 minutes, or any two thereof.

[0048] In some embodiments, after the cap grows to 8-12 cm (e.g., 8, 9, 10, 11, or 12 cm), the mushroom management further includes: stopping watering, ventilating the mushrooms for 20-24 hours, and harvesting after 2-3 days. Specifically, the ventilation time can be any one of 20, 21, 22, 23, or 24 hours, or a range between any two of these.

[0049] The features and performance of the present invention are further described in detail below with reference to the embodiments.

[0050] Example 1. Collection and preservation of wild strains The strain was collected and isolated from the original forests in many places in Nyingchi area, especially in Milin County, Nyingchi City. The strain was isolated from the fruiting body and cultured in PDA medium at 25 degrees Celsius. The strain was molecularly identified and Ganodermaleucocontextum strain CGMCC 5.2226 (its partial DNA sequence is identified as OM721790 in NCBI Genbank) was identified as Ganoderma lucidum after the similarity reached 100%. Ganoderma leucocontextum The strain was deposited in the Culture Collection Center of Guangdong Institute of Microbiology.

[0051] Example 2: Cultivation and domestication process 1. Preparation of seeds for cultivation The first culture medium is as follows: 88% wheat (w / w), 5% bran (w / w), 2% gypsum powder (w / w), 5% quicklime powder (w / w), 60% moisture (w / w), and a pH of 9. All ingredients are thoroughly mixed in a blender for at least 30 minutes. Water should be added while mixing, and the moisture content should be tested. After the ingredients are mixed, the fungus bags are prepared. The specifications for the bags are 15 cm x 30 cm in diameter and length.

[0052] After the bags or bottles are filled, they are placed in an autoclave for sterilization. High-pressure sterilization is performed at a temperature of 125°C and a pressure of 0.15 MPa for 240 minutes. When the temperature in the autoclave drops to 65°C, the sterilized door is opened to cool down. When the temperature drops to 45°C, the bags or bottles are transported to a cooling room. When the temperature drops to 25-28°C, they are transported back to the laboratory for inoculation of the mother culture. The mother culture is then placed in a culture room at an ambient temperature of 22°C and an air humidity of 40%-60%. The bags or bottles are then filled with the culture, and the seeds for cultivation are obtained.

[0053] 2. Cultivation bags and inoculation The second culture medium is formulated as follows: 80% (w / w) Cyclobalanopsis glauca wood shavings, 10% (w / w) bran, 5% (w / w) cornstarch, 2% (w / w) gypsum powder, 3% (w / w) quicklime powder, 62% (w / w) moisture, and a pH of 8-9. After mixing, the mixture is bagged in 17 cm x 33 cm bags and sterilized at 0.15 MPa for 4 hours. The bags are then removed when the temperature in the cabinet drops below 65°C. The solid culture seed (cultivation seed) is transferred in the inoculation room to obtain the bags, which are then inoculated and cultured.

[0054] 3. Culture of bacterial bags Disinfect the culture room with a fog disinfectant such as Guguwang or Gubao. The culture room must be tightly sealed to prevent the fog from escaping. After a day and night of sealing, disinfect again with ozone for 10 hours. Maintain the temperature at 25-26°C and the humidity at 55%-60%. After 7 days, ventilate the room daily for 1 hour. Check the mycelial growth in the room and the bags for trachoma and contamination. If trachoma or individual bags are contaminated, remove them from the room and dispose of them immediately. After 10 days, ventilate the room every 2 hours daily. Check the room daily for growth and address any problems immediately. Once all bags have fully grown mycelium, continue culturing for another 5 days (to obtain mature cultivation bags) to allow them to grow and firm. Only then can they be removed from the room and transported to the greenhouse for harvesting. Qualified bags have neat, white, and strong mycelial growth, are firm, and are free of obvious or hidden contamination or antagonistic lines. Only such bags can be used for cultivation.

[0055] 4. Cultivation and management of chrysanthemums 4.1 Cultivation greenhouse treatment: Before planting white-fleshed Ganoderma lucidum, the greenhouse should be covered with two layers of 95% sunshade net on the outside of the greenhouse film to reduce the temperature and light in the greenhouse. A micro-spraying facility should be installed on the top of the greenhouse to ensure that water is available at any time. The debris in the greenhouse should be cleaned up, the ground should be leveled, and then water should be used to wet the ground. The soil moisture should be between 65% and 70%, and the depth of wetness should not be less than 40 cm. When the surface becomes slightly dry and the soil does not stick to the feet when stepped on, 200 kilograms of quicklime powder should be sprinkled on each acre of new land. For greenhouses that have been used to grow vegetables or fungi, 300 kilograms of quicklime powder should be sprinkled on each acre of land. After the lime is evenly spread, use a micro-tillage machine to turn it over twice. The soil should be fine and there should be no large lumps. Then it can be used for planting.

[0056] 4.2 Planting pattern First, make the bed. The bed is 5 cm deep and 1.5 meters wide, with no length limit. It is set according to the length of the greenhouse. The aisle between the two beds is 0.5 meters wide. In this way, every bed plus the aisle is 2 meters wide. The soil dug out from the bed is placed on the aisle for later use. The bottom of the bed is cleaned and leveled. It must not be uneven or of different widths. The length of the bed is also set according to the length of the greenhouse.

[0057] After the mushroom bags are transported to the greenhouse, they are cultured for another 3-5 days to facilitate the recovery of the mycelium after being injured during transportation. After 3-5 days, peel off one-third of the plastic bag at the bag mouth, and leave the rest intact. When placing the mushroom sticks, cut the end with the plastic facing down, and the other end with the plastic bag facing up. Place them upright in the bed with a spacing of 10-12 cm. When covering with soil, cover the soil to 5 cm from the top of the mushroom stick. The top 5 cm of the mushroom stick is exposed. After 5 days, water it to compact it, add soil to the low parts, and clean the mushroom sticks without mud, sand or debris.

[0058] 4.3 Output Management: On the second day after watering, use scissors to make a hole 1-1.5 cm long, wide and 0.5-1 cm deep in the middle of the top of the mushroom stick. Do not water again within 7 days after opening. Control the temperature and humidity. The temperature is 20-25℃, the humidity is about 50%, and the light intensity is 2,000~5,000 lx. The humidity should not be too high. After the mycelium at the opening recovers, water can be added to increase the humidity only when primordia appear. Remember not to make the humidity too high, 70%~75% is enough. After the primordium grows to the size of a peanut and the opening is completely sealed, the temperature is raised to 25-30℃, the humidity is raised to 80%~90%, and the light intensity is 2,000~5,000 lx.

[0059] After the Ganoderma lucidum buds and grows to the size of peanuts, water it once every three days, with a mist spray for 10-15 minutes, and the temperature should be controlled below 22-30℃. Small ventilation should be provided for 1 hour every day, and the humidity should be maintained between 65% and 85%. The light intensity should be 2,000-5,000 lx. During this period, the main focus is on promoting the growth of the stipe. When the stipe grows to a length of 10-15 cm, increase ventilation and humidity. The temperature should be between 17-20℃ at night and 25-30℃ during the day. The vents should be opened all day for ventilation. In order to speed up the growth of Ganoderma lucidum, the vents can be closed at night to keep warm, or a small gap should be left in the vents to achieve the purpose of insulation. During this period, Ganoderma lucidum is managed with large ventilation and high humidity to inhibit the growth of the stipe and promote the differentiation and growth of the cap. Water should be sprayed once in the morning and evening for 10-15 minutes each time. When the cap grows to about 10 cm, has no yellow edges, basically turns red or dark red with a little white edge around it, and no spores are ejected or only a small amount of spores are ejected, stop spraying water, open all the vents during the day and night to reduce the humidity of the Ganoderma lucidum fruiting body, and it can be harvested after three days.

[0060] 4.4 Harvesting The first batch of Ganoderma lucidum needs to be managed for about 60 days from budding to harvesting. Do not spray water within 5-7 days after harvesting the first batch of Ganoderma lucidum to allow the wounds from which the Ganoderma lucidum has been harvested to heal. After 5-7 days, when the wound surface is covered with hyphae or primordia appear, normal management during the budding period can be resumed. The second batch of Ganoderma lucidum can be harvested after about 30 days, and the third batch can be managed in the same way as the second batch, and the third batch can also be harvested after 30 days.

[0061] Example 3, Cultivation Evaluation Results The cultivation method provided in Example 2 was used to cultivate the plants in the vicinity of Lhasa (3900 meters above sea level). Figure 1 and Figure 2 .

[0062] 1. Appearance and shape The cap is semicircular, kidney-shaped or nearly circular, with regular shape, as shown in the picture. The cap diameter is 15-25 cm and the average thickness is 0.95 cm.

[0063] The stipe is lateral or occasionally eccentric, 5-12 cm long, 0.5-3.2 cm thick, purple-brown and shiny.

[0064] 2. Production statistics The wild strain TGL10 demonstrates good adaptability to the plateau, boasts excellent appearance and high yield, with an average yield of 139.93 grams per bag (wet weight) and a conversion rate of nearly 27.99%, exceeding that of other strains currently available. High-quality (kidney-shaped and nearly round) varieties account for over 90%, demonstrating advantages in both yield and quality. The strain LE1, commonly used in production, has an average yield of 95.34 grams (wet weight) and a conversion rate of 19.07%.

[0065] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A white-fleshed Ganoderma lucidum strain, characterized in that: It is deposited in the Culture Collection Center of Guangdong Institute of Microbiology and its Latin taxonomic name is Ganoderma leucocontextum , the white-fleshed Ganoderma lucidum strain TGL10 with a deposit number of GDMCC No: 66044 or its progeny.

2. The white-fleshed Ganoderma lucidum strain according to claim 1, characterized in that The nucleotide sequence identity between the genome sequence of the offspring and the genome sequence of the white-fleshed Ganoderma lucidum strain TGL10 is ≥80%.

3. A bacterial agent, characterized in that It contains the white-fleshed Ganoderma lucidum strain according to claim 1 or 2.

4. A fermentation product, characterized in that It contains the white-fleshed Ganoderma lucidum strain according to claim 1 or 2.

5. A method for cultivating white-fleshed Ganoderma lucidum, characterized in that: It includes: The white-fleshed Ganoderma lucidum strain according to claim 1 or 2 is cultivated.

6. The cultivation method according to claim 5, characterized in that The cultivation method comprises the following steps: inoculating the mother strain of the white-fleshed Ganoderma lucidum strain according to claim 1 or 2 into a first culture medium for expansion culture to obtain seeds for cultivation; inoculating the cultivation seeds into a cultivation bag containing a second culture matrix, and culturing until the mycelium is fully grown to obtain a mature cultivation bag; The mature cultivation bags are cultivated and managed to obtain fruiting bodies.

7. The cultivation method according to claim 6, characterized in that The first culture matrix includes the following components and their weight percentages: 85% to 95% wheat, 1% to 10% bran, 1% to 5% gypsum powder and 1% to 10% quicklime powder, with a water content of 50% to 70% and a pH of 9 to 10.

8. The cultivation method according to claim 6, wherein The second culture matrix includes the following components and the weight percentages of the components: 75% to 85% wood chips, 5% to 15% bran, 1% to 10% corn flour, 1% to 5% gypsum powder and 1% to 5% quicklime powder, with a water content of 50% to 70% and a pH of 8 to 9.

9. The cultivation method according to claim 7 or 8, characterized in that The wood cuttings include Cyclobalanopsis glauca wood cuttings.

10. The cultivation method according to claim 6, wherein The cultivation conditions of the cultivation bag include: a temperature of 25-26° C. and a humidity of 55%-60%.

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