Pleurotus citrinopileatus strain as well as cultivation method and application thereof

Abstract

The invention discloses a pleurotus citrinopileatus strain as well as a cultivation method and application thereof, and belongs to the technical field of microorganisms. According to the present invention, the Pleurotus dammanus strain is a Pleurotus dammanus ZGCL24-8-16-7 strain, and the Pleurotus dammanus ZGCL24-8-16-7 strain is a Pleurotus dammanus strain; the preservation number of the pleurotus citrinopileatus ZGCL24-8-16-7 strain is CGMCC (China General Microbiological Culture Collection Center) No.41900. The pleurotus citrinopileatus The pleurotus citrinopileatus ZGCL24-8-16-7 strain disclosed by the invention has the advantages that the hypha growth speed is high; the bag filling time of hyphae is 15-20 days, the fungus age is short, the fungus culture time is shortened, and the fungus culture cost is reduced. The sporocarp cultivated by the method is clustered and light beige, the yield of a single bag is 135.27-156.5 g (200g dry material), and the biotransformation rate is 67.6%-78.3%. The pleurotus eryngii strain sporocarp obtained through cultivation has the high nutritional value, the crude fiber content is high, the hardness is large, and storage and transportation are convenient.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Pleurotus ostreatus strain, a cultivation method and an application thereof. Background Art

[0002] Pleurotus ostreatus is a rare edible fungus. It is not only rich in nutrients such as protein and amino acids, but also rich in various active ingredients such as polysaccharides. It has multiple functions such as anti-tumor and immune regulation. At the same time, it participates in material circulation in the ecosystem and has important ecological functions.

[0003] Currently, there are relatively few resources of Pleurotus eryngii strains, and the market lacks high-quality, high-yield strains. Furthermore, numerous issues exist with regard to the germplasm resources of Pleurotus eryngii. On the one hand, wild germplasm resources are being impacted by human activities and ecological changes, resulting in a shrinking distribution range and a continuous decline in population size, posing severe challenges to their native genetic diversity. On the other hand, artificially cultivated strains suffer from immature breeding techniques and a lack of systematic, scientific germplasm resource banks, resulting in existing strains suffering from a single genetic background and varying quality.

[0004] Therefore, it is urgent to discover and screen new strains of Pleurotus ostreatus with excellent characteristics, provide new materials for the selection and breeding of excellent Pleurotus ostreatus strains and the protection of germplasm resources, assist in the scientific protection and efficient utilization of Pleurotus ostreatus strain resources, and assist its industrialization development process. Summary of the Invention

[0005] In view of this, the object of the present invention is to provide a Pleurotus erythrorhizome strain with fast mycelial growth rate, short bacterial age, high bioconversion rate, light beige fruiting body, high nutritional value, and easy storage and transportation.

[0006] Another object of the present invention is to provide a cultivation method of the Pleurotus ostreatus strain.

[0007] Another object of the present invention is to provide an application of the Pleurotus ostreatus strain or the fruiting body of the Pleurotus ostreatus ZGCL24-8-16-7 strain obtained by the cultivation method in food.

[0008] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0009] The invention provides a Pleurotus djamor strain, which is a Pleurotus djamor ZGCL24-8-16-7 strain; the preservation number of the Pleurotus djamor ZGCL24-8-16-7 strain is CGMCC No.41900.

[0010] The present invention also provides a cultivation method of the Pleurotus ostreatus strain, which comprises: inoculating the mother strain of the Pleurotus ostreatus ZGCL24-8-16-7 strain into a stock culture medium for cultivation to obtain a solid stock; inoculating the solid stock into a cultivation seed bag, culturing to obtain a cultivated seed; opening the lid of the cultivation seed bag, inducing mushroom production, and obtaining a fruiting body of the Pleurotus ostreatus ZGCL24-8-16-7 strain.

[0011] Preferably, the stock culture medium comprises the following raw materials: potato, sucrose and ammonium sulfate.

[0012] Preferably, based on 1 L, the stock culture medium includes the following raw materials: 100-300 g of potato, 20-30 g of sucrose and 1-3 g of ammonium sulfate.

[0013] Preferably, the culture conditions of the mother strain of Pleurotus ostreatus ZGCL24-8-16-7 include: culturing in the dark at 25-27° C. for 6-9 days.

[0014] Preferably, the cultivation spawn bag comprises the following raw materials: miscellaneous sawdust, wheat bran, quicklime and gypsum.

[0015] Preferably, the cultivation spawn bag comprises the following raw materials in parts by weight: 70-90 parts of sawdust, 10-20 parts of wheat bran, 0.5-3 parts of quicklime and 0.5-3 parts of gypsum.

[0016] Preferably, the culture conditions of the solid stock include: temperature of 23-32° C., humidity of 40%-50%, and culture in the dark for 15-20 days.

[0017] Preferably, the fruiting conditions include: temperature of 20-25° C., humidity of 85%-95%, light intensity of 100-600 Lux, and fruiting time of 25-30 days.

[0018] The present invention also provides an application of the Pleurotus ostreatus strain or the fruiting body of the Pleurotus ostreatus ZGCL24-8-16-7 strain obtained by the cultivation method in food.

[0019] Beneficial effects of the present invention:

[0020] The mycelium of the Pleurotus ostreatus ZGCL24-8-16-7 strain of the present invention is white and dense, and the mycelium growth rate can reach 11.40±0.70 mm / d, with a fast mycelium growth rate; the mycelium takes 15 to 20 days to fill the bag, and the mycelium age is short, which shortens the culture time and reduces the culture cost. The fruiting bodies produced by the cultivation of the present invention are clustered, light beige, and have a trumpet-shaped and funnel-shaped shape. The yield per bag is 135.27 to 156.5 g (200 g dry material), and the bioconversion rate is 67.6% to 78.3%. The fruiting bodies of the Pleurotus ostreatus ZGCL24-8-16-7 strain obtained by the cultivation of the present invention have high contents of polysaccharides, crude protein and crude fiber, and have high nutritional value. At the same time, due to the high crude fiber content, the fruiting bodies are hard, which is convenient for storage and transportation. They are also beneficial to the intestines and have a digestion-promoting effect.

[0021] Biological deposit information

[0022] The Pleurotus djamor ZGCL24-8-16-7 strain of the present invention is classified and named Pleurotus djamor, and is deposited in the General Microbiology Center of the China Culture Collection Administration (CGMCC for short). The deposit time is April 24, 2025, and the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the deposit number is CGMCC No. 41900. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The wild Pleurotus rosa-sinensis fruiting body in Example 1;

[0024] Figure 2 The mycelial morphological characteristics of the Pleurotus ostreatus ZGCL24-8-16-7 strain in Example 1;

[0025] Figure 3 The basidiospore morphological characteristics of the Pleurotus ostreatus ZGCL24-8-16-7 strain in Example 1;

[0026] Figure 4 This is the ITS sequence phylogenetic tree of the Pleurotus ostreatus ZGCL24-8-16-7 strain constructed based on the neighbor-joining method in Example 1;

[0027] Figure 5 This is the LSU sequence phylogenetic tree of the Pleurotus ostreatus ZGCL24-8-16-7 strain constructed based on the neighbor-joining method in Example 1;

[0028] Figure 6 This is a photo of the cultivation and fruiting of the Pleurotus ostreatus ZGCL24-8-16-7 strain in Example 3;

[0029] Figure 7These are the nutrient components of the fruiting body of the Pleurotus ostreatus ZGCL24-8-16-7 strain in Example 3. DETAILED DESCRIPTION

[0030] The invention provides a Pleurotus djamor strain, which is a Pleurotus djamor ZGCL24-8-16-7 strain; the preservation number of the Pleurotus djamor ZGCL24-8-16-7 strain is CGMCC No.41900.

[0031] The Pleurotus djamori ZGCL24-8-16-7 strain of the present invention was collected from Zhangguangcai Ridge, Shangzhi City, Heilongjiang Province in September 2024. Pure strains were obtained through tissue separation and purification. It was identified as Pleurotus djamor and was deposited in the General Microbiology Center of the China Culture Collection Administration on April 24, 2025, with the deposit number CGMCC No. 41900.

[0032] The mycelia of the Pleurotus ostreatus ZGCL24-8-16-7 strain of the present invention are white and dense, the basidiospores are rod-shaped, and the size of the basidiospores is 5.2-6.8 μm×2.6-3.2 μm.

[0033] In the present invention, the culture method of the Pleurotus ostreatus ZGCL24-8-16-7 strain preferably includes: inoculating the Pleurotus ostreatus ZGCL24-8-16-7 strain into a culture medium for culture. The carbon source of the culture medium preferably includes sucrose, the nitrogen source preferably includes ammonium sulfate, the pH is preferably 7.5 to 8.5, for example, 7.5, 8 or 8.5, and the culture temperature is preferably 25 to 30°C, for example, 25, 26, 27, 28, 29 or 30°C; in some embodiments, the culture medium preferably includes 100 to 300 g / L of potato, 20 to 30 g / L of sucrose, 1 to 3 g / L of ammonium sulfate and 0.1 to 1 g / L of potassium dihydrogen phosphate; the content of the potato is preferably 100 to 300 g / L, for example, 100 , 150, 200, 250 or 300 g / L; the sucrose content is preferably 20-30 g / L, such as 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 g / L; the ammonium sulfate content is preferably 1-3 g / L, such as 1, 1.5, 2, 2.5 or 3 g / L; the potassium dihydrogen phosphate content is preferably 0.1-1 g / L, such as 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 or 1 g / L. The mycelium growth rate of the Pleurotus ostreatus ZGCL24-8-16-7 strain can reach 11.40±0.70 mm / d, which is faster than the mycelium growth rate of other strains of the same genus (average 8 mm / d).

[0034] The present invention also provides a cultivation method of the Pleurotus ostreatus strain, which comprises: inoculating the mother strain of the Pleurotus ostreatus ZGCL24-8-16-7 strain into a stock culture medium for cultivation to obtain a solid stock; inoculating the solid stock into a cultivation seed bag, culturing to obtain a cultivated seed; opening the lid of the cultivation seed bag, inducing mushroom production, and obtaining a fruiting body of the Pleurotus ostreatus ZGCL24-8-16-7 strain.

[0035] In the present invention, the method for preparing the strain mother culture can be conventionally selected according to actual needs. In some embodiments, the method for preparing the strain mother culture preferably includes: inoculating the Pleurotus eryngii ZGCL24-8-16-7 strain into a cultivation medium, culturing, and obtaining the strain mother culture. In the present invention, the culturing temperature is preferably 23 to 27°C, for example, 23, 24, 25, 26, or 27°C; the culturing time can be conventionally selected according to actual needs, preferably culturing until the mycelium is fully grown, preferably 8 to 12 days, for example, 8, 9, 10, 11, or 12 days. The cultivation material preferably includes the following raw materials: miscellaneous wood chips, wheat bran, quicklime and gypsum; as an implementable embodiment, the cultivation material preferably includes the following raw materials in parts by weight: 85-100 parts of miscellaneous wood chips, 2-8 parts of wheat bran, 0.5-2 parts of quicklime and 0.5-2 parts of gypsum; further preferably includes the following raw materials in parts by weight: 90-95 parts of miscellaneous wood chips, 4-6 parts of wheat bran, 0.8-1.5 parts of quicklime and 0.8-1.5 parts of gypsum; in some embodiments, the cultivation material preferably includes the following raw materials in parts by weight: 93 parts of miscellaneous wood chips, 5 parts of wheat bran, 1 part of quicklime and 1 part of gypsum.

[0036] In the present invention, the stock culture medium preferably comprises the following raw materials: potato, sucrose, and ammonium sulfate. As an embodiment, based on 1 L, the stock culture medium preferably comprises the following raw materials: 100-300 g potato, 20-30 g sucrose, and 1-3 g ammonium sulfate; further preferably comprises the following raw materials: 150-250 g potato, 22-28 g sucrose, and 1.5-2.5 g ammonium sulfate. In some embodiments, based on 1 L, the stock culture medium preferably comprises: 200 g potato, 25 g sucrose, and 2.0 g ammonium sulfate. The pH of the stock culture medium is preferably 7.5-8.5, for example, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, or 8.5.

[0037] In the present invention, the culture temperature of the mother strain of Pleurotus ostreatus ZGCL24-8-16-7 is preferably 25-27°C, for example, 25, 26 or 27°C; the culture time is preferably 6-9 days, for example, 6, 7, 8 or 9 days; and the culture is preferably carried out in the dark.

[0038] In the present invention, preferably, solid stock can be obtained when the mycelium has grown all over the culture bottle.

[0039] In the present invention, the cultivation seed bag preferably includes the following raw materials: miscellaneous wood chips, wheat bran, quicklime and gypsum; as an implementable embodiment, the cultivation seed bag preferably includes the following raw materials in parts by weight: 70-90 parts of miscellaneous wood chips, 10-20 parts of wheat bran, 0.5-3 parts of quicklime and 0.5-3 parts of gypsum; further preferably includes the following raw materials in parts by weight: 75-85 parts of miscellaneous wood chips, 12-18 parts of wheat bran, 0.8-2 parts of quicklime and 0.8-2 parts of gypsum; in some embodiments, the cultivation seed bag preferably includes the following raw materials in parts by weight: 83 parts of miscellaneous wood chips, 15 parts of wheat bran, 1 part of quicklime and 1 part of gypsum.

[0040] In the present invention, the culture temperature of the solid stock is preferably 23-32°C, for example, 23, 24, 25, 26, 27, 28, 29, 30, 31 or 32°C; the humidity is preferably 40%-50%, for example, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49% or 50%; the culture time is preferably 15-20d, for example, 15, 16, 17, 18, 19 or 20d; and the culture is preferably carried out in the dark.

[0041] In the present invention, the fruiting temperature is preferably 20-25°C, for example, 20, 21, 22, 23, 24 or 25°C; the humidity is preferably 85%-95%, for example, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94% or 95%; the light intensity is preferably 100-600 Lux, for example, 100, 200, 300, 400, 500 or 600 Lux; and the fruiting time is preferably 25-30 days, for example, 25, 26, 27, 28, 29 or 30 days.

[0042] The mycelium of the strain cultured by the cultivation method of the present invention grows fast, the time for the mycelium to fill a bag is 15 to 20 days, the mycelium age is short, the culture time is shortened, and the culture cost is reduced; the obtained fruiting body of the Pleurotus ostreatus ZGCL24-8-16-7 strain is clustered and light beige, forming a clear contrast with the traditional Pleurotus ostreatus fungus with a pink color, and is trumpet-shaped and funnel-shaped, with a single bag yield reaching 135.27 to 156.5 g (200 g dry material) and a bioconversion rate of 67.6% to 78.3%; the fruiting body has a total sugar content of 71.7%, a protein content of 21.6%, a crude fiber content of 3.3%, and a crude fat content of 1.1%, and compared with the fruiting body of Pleurotus ostreatus No. 1 of the same genus, the crude protein and crude fiber contents are high, not only has high nutritional value, but also has high crude fiber content, is hard, is convenient for storage and transportation, is beneficial to the intestines, and has a digestion-promoting effect.

[0043] The present invention also provides an application of the Pleurotus ostreatus strain or the fruiting body of the Pleurotus ostreatus ZGCL24-8-16-7 strain obtained by the cultivation method in food.

[0044] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0045] In the following examples, unless otherwise specified, all methods are conventional.

[0046] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0047] Example 1

[0048] In September 2024, a wild fruiting body of Pleurotus rosaceus was collected from Zhangguangcailing, Shangzhi City, Heilongjiang Province ( Figure 1 ), the strain was obtained as a pure strain through tissue separation and purification.

[0049] The mycelial morphological characteristics of the pure strain obtained in this example are as follows Figure 2 As shown in the figure, it can be seen that the mycelium of this strain is white and dense. Figure 3 As shown, it can be seen that the basidiospores are rod-shaped and the size of the basidiospores is 5.2-6.8 μm × 2.6-3.2 μm.

[0050] Phylogenetic tree of ITS and LSU sequences of Pleurotus rosaceae constructed based on the neighbor-joining method is shown in the figure. Figure 4 、 Figure 5 As shown, the strain was identified as Pleurotus djamor, named Pleurotus djamor ZGCL24-8-16-7, and deposited in the General Microbiology Center of China Culture Collection Administration on April 24, 2025, with the deposit number CGMCC No.41900.

[0051] Example 2

[0052] The Pleurotus rosaceae strain ZGCL24-8-16-7 was inoculated into culture media containing different carbon sources (no carbon source, starch, mannitol, fructose, dextrin, lactose, and sucrose) at 25°C. The culture medium formulation consisted of 200 g / L potato, 20 g / L carbon source, 3 g / L peptone, and 0.5 g / L potassium dihydrogen phosphate, with a natural pH. The results showed that mycelial growth was fastest when sucrose was used as the carbon source, at 10.95 ± 1.32 mm / day.

[0053] The Pleurotus rosaceae strain ZGCL24-8-16-7 was inoculated into culture media containing different nitrogen sources (no nitrogen source, ammonium sulfate, L-arginine, yeast enzymes, urea, and potassium nitrate). Cultures were performed at 25°C using the following medium: 200g / L potato, 20g / L glucose, 3g / L nitrogen source, 0.5g / L potassium dihydrogen phosphate, and a natural pH. Results showed that mycelial growth was fastest when ammonium sulfate was used as the nitrogen source, at 6.80±0.96mm / day.

[0054] The Pleurotus ostreatus strain ZGCL24-8-16-7 was inoculated and cultured at different temperatures (15°C, 20°C, 25°C, 30°C, and 35°C) in a medium containing 200g / L potato, 20g / L glucose, 3g / L peptone, and 0.5g / L potassium dihydrogen phosphate, with a natural pH. Results showed that mycelial growth was fastest at 30°C, reaching 7.88±1.31mm / day.

[0055] The Pleurotus ostreatus strain ZGCL24-8-16-7 was inoculated into culture media at different pH values ​​(natural, 4, 5, 6, 7, and 8) at 25°C. The culture medium consisted of 200 g / L potato, 20 g / L glucose, 3 g / L peptone, and 0.5 g / L potassium dihydrogen phosphate. Results showed that mycelial growth was fastest at pH 8, reaching 10.05 ± 1.14 mm / day.

[0056] Based on the experimental results of the strain's optimal carbon source, nitrogen source, temperature, and pH for mycelial growth rate and vigor, an orthogonal experiment was conducted using sucrose as the carbon source and ammonium sulfate as the nitrogen source. The results of the orthogonal experiment revealed that the key factors affecting mycelial growth of Pleurotus truncatula were ranked in the following order: temperature (D) > nitrogen source (B) > carbon source (A) > pH (C). The optimal culture medium formulation, determined by range analysis, was: 200 g / L potato, 25 g / L sucrose, 2.0 g / L ammonium sulfate, 0.5 g / L potassium dihydrogen phosphate, a medium pH of 8, and a culture temperature of 30°C. Under these conditions, the mycelial growth rate was 11.40 ± 0.70 mm / day.

[0057] Example 3 Acclimation and cultivation of Pleurotus ostreatus ZGCL24-8-16-7 strain

[0058] Preparation of the mother strain: The Pleurotus ostreatus ZGCL24-8-16-7 strain was inoculated into the cultivation material and cultured at 25°C for 10 days. After the mycelium was fully grown, the mother strain was obtained and stored in a 4°C refrigerator for later use; the formula of the cultivation material was (mass percentage): 93% sawdust, 5% wheat bran, 1% quicklime and 1% gypsum.

[0059] Preparation of solid stock culture: Inoculate the strain mother culture into the stock culture medium and culture in the dark at 25°C for 9 days. When the mycelium quickly fills the culture bottle, the solid stock culture can be obtained. The stock culture medium formula is: 200g / L potato, 25g / L sucrose, 2.0g / L ammonium sulfate, pH 8.0.

[0060] Preparation of cultivated seeds: The solid original seeds were inoculated into a cultivated seed bag that had been autoclaved at 121°C for 2 hours. The culture temperature was 25°C, the culture humidity was 45%, and the culture was kept away from light for 17 days to obtain the cultivated seeds. The formula of the cultivated seed bag was (mass percentage): 83% sawdust, 15% wheat bran, 1% quicklime, and 1% gypsum.

[0061] Cultivation and fruiting: Open the lid of the cultivation spawn bag and place it in a fruiting room at 25°C, with a fruiting humidity of 90% and a light intensity of 400 Lux. Fruiting was carried out for 27 days to obtain the fruiting bodies of the Pleurotus ostreatus ZGCL24-8-16-7 strain.

[0062] Fruiting bodies obtained from cultivation Figure 6 As shown, it can be seen that the fruiting bodies cultivated from the ZGCL24-8-16-7 strain of Pleurotus ostreatus are clustered, light beige, trumpet-shaped and funnel-shaped, which are obviously different from the fruiting bodies of Pleurotus ostreatus No. 1 of the same genus.

[0063] According to the test, the yield of a single bag is 135.27-156.5 g (200 g dry material), and the bioconversion rate is 67.6%-78.3%.

[0064] The nutrient content in the fruiting bodies of the Pleurotus ostreatus ZGCL24-8-16-7 strain and the Pleurotus ostreatus No. 1 strain was detected respectively. The results are as follows Figure 7 The results showed that the fruiting bodies cultivated with the Pleurotus ostreatus strain ZGCL24-8-16-7 contained 71.7% total sugar, 21.6% protein, 3.3% crude fiber, and 1.1% crude fat. The fruiting bodies differed in nutrient content from those of the same genus, Pleurotus ostreatus No. 1, with higher crude protein and crude fiber contents.

[0065] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A Pleurotus erythrorhizome strain, characterized in that: The Pleurotus djamor strain is the Pleurotus djamor ZGCL24-8-16-7 strain; the preservation number of the Pleurotus djamor ZGCL24-8-16-7 strain is CGMCC No.41900.

2. The cultivation method of the Pleurotus ostreatus strain according to claim 1, characterized in that: The cultivation method comprises: inoculating the mother strain of the Pleurotus ostreatus ZGCL24-8-16-7 strain into a stock culture medium for cultivation to obtain a solid stock; inoculating the solid stock into a cultivation seed bag, culturing to obtain a cultivated seed; and opening the lid of the cultivation seed bag to induce mushroom production to obtain a fruiting body of the Pleurotus ostreatus ZGCL24-8-16-7 strain.

3. The cultivation method according to claim 2, wherein The stock culture medium comprises the following raw materials: potato, sucrose and ammonium sulfate.

4. The cultivation method according to claim 3, wherein Based on 1 L, the stock culture medium includes the following raw materials: 100-300 g of potato, 20-30 g of sucrose and 1-3 g of ammonium sulfate.

5. The cultivation method according to claim 2, wherein The culture conditions of the mother strain of Pleurotus ostreatus ZGCL24-8-16-7 include: culturing in the dark at 25-27° C. for 6-9 days.

6. The cultivation method according to claim 2, wherein The cultivation spawn bag comprises the following raw materials: miscellaneous sawdust, wheat bran, quicklime and gypsum.

7. The cultivation method according to claim 6, characterized in that The cultivation spawn bag comprises the following raw materials in parts by weight: 70-90 parts of miscellaneous sawdust, 10-20 parts of wheat bran, 0.5-3 parts of quicklime and 0.5-3 parts of gypsum.

8. The cultivation method according to claim 2, wherein The culture conditions of the solid stock include: temperature of 23-32° C., humidity of 40%-50%, and culture in the dark for 15-20 days.

9. The cultivation method according to claim 2, wherein The mushroom fruiting conditions include: temperature of 20-25° C., humidity of 85%-95%, light intensity of 100-600 Lux, and mushroom fruiting time of 25-30 days.

10. Use of the Pleurotus ostreatus strain according to claim 1 or the fruiting body of the Pleurotus ostreatus ZGCL24-8-16-7 strain obtained by the cultivation method according to any one of claims 2 to 9 in food.

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