Bacillus subtilis SXDYYB7 and application thereof in prevention and control of piglet coccidiosis

Abstract

The invention belongs to the technical field of prevention and control of piglet coccidiosis, relates to bacillus subtilis SXDYYB7 and application thereof in prevention and control of piglet coccidiosis, provides bacillus subtilis SXDYYB7 with the preservation number of CCTCC NO: M2025256, and further provides a composition for prevention and control of piglet coccidiosis, and the composition is prepared from the following dry medicinal materials in parts by weight: 20-30 parts of pomegranate, 2-3 parts of verbena, 1-3 parts of bacillus subtilis SXDYYB7, 1-3 parts of bacillus subtilis SXDYYB7 and 1-3 parts of bacillus subtilis SXDYYB7. The feed additive is prepared from the following raw materials in parts by weight: 10-20 parts of radix glycyrrhizae, 20-30 parts of radix sophorae flavescentis, 10-30 parts of rhizoma coptidis and 1-3 parts of an SXDYYB7 microbial preparation. The traditional Chinese medicine composition is prepared by boiling pomegranate, verbena, liquorice, radix sophorae flavescentis and coptis chinensis in water at normal temperature to obtain liquid medicine and then adding an SXDYYB7 microbial preparation. The composition for preventing and treating the piglet coccidiosis can play a good role in preventing and treating the piglet coccidiosis.

Description

Technical Field

[0001] The invention belongs to the technical field of piglet coccidiosis prevention and control, and relates to Bacillus subtilis SXDYYB7 and application thereof in preventing and controlling piglet coccidiosis. Background Art

[0002] Pork is a key indicator of improving national living standards and a significant factor influencing price fluctuations. Piglets are most susceptible to coccidiosis after birth. This protozoan disease, caused by Eimeria and Isospora parasites that infect the intestinal epithelial cells of lactating and recently weaned pigs, primarily occurs in lactating piglets between 7 and 15 days of age. Initially, diarrhea is observed in a small number of piglets, with yellow, yellowish-white, or brownish-brown stools being the predominant color. Some affected pigs may also have fecal matter mixed with mucus and blood, with a creamy, porridge-like, or watery consistency, and their bodies may be covered in liquid feces. As the disease progresses, some affected pigs may develop fecal incontinence, frequently pass loose, bloody feces with a foul odor that stains the hindquarters, and experience redness, swelling, and straining around the anus. Affected pigs grow slowly, with uneven growth and development among littermates. Diarrhea typically persists for 3 to 8 days, with a morbidity rate of 50% to 70%. Some piglets recover on their own, becoming carriers of the parasite and continuously excreting coccidia oocysts, becoming a source of infection. Severe diarrhea can lead to piglet death if not treated promptly. Prevention is paramount for piglet coccidiosis, but effective preventive and therapeutic medications are currently unavailable. Chemical antibiotics have a killing and inhibitory effect on piglet coccidia and are generally the preferred treatment for coccidiosis. However, piglet coccidia are highly susceptible to developing resistance to chemical antibiotics such as coccidiostat, mefenamic acid, albendazole, and chlorpheniramine hydrochloride. Even with the planned alternating or combined use of multiple anticoccidial drugs for treatment or prevention, resistance cannot be completely avoided.

[0003] Within the trove of traditional Chinese medicine, there are numerous Chinese herbs with excellent disease-fighting properties. The mechanisms by which these herbs work against coccidia are primarily described as follows: 1. Antiparasitic Effects - Many Chinese herbal medicines contain antiparasitic active ingredients that can directly kill or inhibit the growth and reproduction of coccidia. For example, Chinese herbs such as Sophora flavescens, Coptis chinensis, and Phellodendron amurense contain alkaloids that can destroy the cell membranes of coccidia, leading to their death. 2. Improving immunity - Chinese herbs can enhance piglets' resistance to coccidia by regulating their immune systems. For example, Chinese herbs such as ginseng, astragalus, and Codonopsis pilosula can promote the proliferation and activation of immune cells, improving the body's immune function, thereby reducing coccidia infection and disease. 3. Improving the intestinal microenvironment - Chinese herbs can also inhibit the growth of coccidia by improving the intestinal microenvironment. For example, Chinese herbs such as hawthorn, tangerine peel, and Poria cocos can promote the secretion of digestive juices, improve intestinal peristalsis, and reduce the breeding environment for coccidia. 4. Anti-inflammatory effects - Coccidia infection can cause intestinal inflammation. Certain components of Chinese herbs have anti-inflammatory effects, which can reduce inflammatory responses and alleviate symptoms. For example, Chinese herbs such as white peony root and licorice contain anti-inflammatory components that can inhibit the release of inflammatory mediators and reduce intestinal inflammation. 5. Comprehensive conditioning - Chinese herbal medicines usually have multiple functions and can comprehensively regulate the physical condition of piglets and improve their overall health level; for example, Chinese herbal medicines such as angelica and Chuanxiong can replenish blood, improve blood circulation, and enhance the body's disease resistance.

[0004] Probiotics are live microorganisms that have a beneficial effect on the human body by improving the balance of intestinal flora. They are also known as microecological preparations. They have developed rapidly in the past 10 years and are a research hotspot in nutrition. Their main function is to help establish a gastrointestinal microbial flora that is beneficial to the host through the competitive exclusion of digestive tract organisms, prevent bacterial, viral and coccidia diarrhea, and promote growth. Their main mechanisms of action are: 1. Competitive inhibition: probiotics can compete with coccidia for attachment sites and nutrients in the intestine, thereby inhibiting the growth and reproduction of coccidia; 2. Enhance immunity : Probiotics can stimulate the immune system of piglets and improve their resistance to coccidia; by regulating the balance of intestinal microecology, probiotics help enhance the overall immunity of piglets; 3. Improve the intestinal environment: Probiotics can produce organic acids (such as lactic acid and acetic acid), lower the pH value of the intestine, and create an environment that is not conducive to the survival of coccidia; in addition, probiotics can promote the growth of beneficial bacteria and inhibit the reproduction of harmful bacteria; 4. Inhibit pathogenic microorganisms: Probiotics can produce antibacterial substances, such as bacteriocins and hydrogen peroxide, which can inhibit the growth of coccidia and other pathogenic microorganisms.

[0005] Although Chinese herbal medicine or probiotics can improve the intestinal microecological environment of piglets and enhance their immunity, for serious infections that have already occurred, especially piglet diarrhea caused by coccidia, the use of Chinese herbal medicine or probiotics alone may not achieve ideal prevention and control effects. Summary of the Invention

[0006] In order to solve the above technical problems, the present invention provides a Bacillus subtilis SXDYYB7 and its application in preventing and controlling piglet coccidiosis. The composition of the microbial preparation including Bacillus subtilis SXDYYB7 can play a good preventive and therapeutic role in piglet coccidiosis.

[0007] The technical solution of the present invention to solve the above technical problems is as follows: The present invention provides a Bacillus subtilis ( Bacillus subtilis )SXDYYB7, the deposit number is CCTCCNO:M2025256.

[0008] The present invention also provides the above-mentioned Bacillus subtilis ( Bacillus subtilis ) Application of SXDYYB7 in the prevention and treatment of piglet coccidiosis.

[0009] Furthermore, the application includes using the Bacillus subtilis ( Bacillus subtilis )SXDYYB7 and / or its metabolites.

[0010] The present invention also provides a composition for preventing and treating piglet coccidiosis, wherein the composition comprises the Bacillus subtilis ( Bacillus subtilis )SXDYYB7 microbial preparation made from SXDYYB7.

[0011] Furthermore, the composition is composed of the following dry medicinal raw materials: pomegranate, verbena, licorice, sophora flavescens, coptis chinensis, and SXDYYB7 microbial preparation.

[0012] Pomegranate: Latin name Pomegranate L. . ， It is sweet, sour, astringent and warm in nature, and has insecticidal, astringent, intestinal astringent and dysentery-relieving effects; Verbena: Latin name Verbena officinalis L. , cool in nature, with a slightly pungent taste, it has the effects of cooling blood, dispersing blood stasis, promoting menstruation, clearing away heat, detoxifying, relieving itching, expelling parasites, and relieving swelling; Licorice: Latin name Licorice Root It is flat in nature, sweet in taste, with a slight smell and a special sweet taste. It is mainly used to clear away heat and detoxify, eliminate phlegm and relieve cough, and relieve abdominal distension. Sophora flavescens: Latin name Sophora flavense Bitter, cold, enters the heart, liver, stomach, large intestine, and bladder meridians; clears heat and dampness, kills parasites, and is a diuretic; used for heat dysentery, bloody stools, jaundice, urinary retention, leucorrhea, vaginal swelling and itching, eczema, wet sores, itchy skin, scabies, leprosy; Coptis chinensis: Latin name Coptis Rhizome ; It tastes extremely bitter, clears away heat and dampness, and relieves fire and detoxifies.

[0013] SXDYYB7 has strong protease, lipase, amylase and other activities, can produce antibacterial substances, has strong biological oxygen-snatching ability in the animal intestine, and can produce L-lactic acid under anaerobic conditions. These characteristics play an important role in promoting the digestion and absorption of animal nutrients, improving the feed conversion rate of animals, preventing diseases and promoting growth.

[0014] Furthermore, the composition is composed of the following dry medicinal materials in parts by weight: 20-30 parts of pomegranate, 2-3 parts of verbena, 10-20 parts of licorice, 20-30 parts of sophora flavescens, 10-30 parts of coptis chinensis, and 1-3 parts of SXDYYB7 microbial preparation.

[0015] Furthermore, the preparation method of the SXDYYB7 microbial preparation is: the Bacillus subtilis ( Bacillus subtilis ) SXDYYB7 seed liquid is inoculated into a fermentation medium, and after continuous culture at 31-35°C for 24-72 hours, it is statically cultured for 96-120 hours to obtain a fermentation liquid; the fermentation liquid is centrifuged to collect the bacteria, and after freeze-drying, the SXDYYB7 microbial preparation is obtained.

[0016] Furthermore, the fermentation medium is composed of the following components in parts by weight: 1-5 parts of fish meal, 1-3 parts of corn flour, 1-10 parts of yeast extract, 5-15 parts of sodium chloride, 10-100 parts of soybean meal powder, and 1000 parts of water.

[0017] Further preferably, the fermentation medium consists of the following components in parts by weight: 3 parts of fish meal, 2 parts of corn flour, 5 parts of yeast extract, 10 parts of sodium chloride, 50 parts of soybean meal powder, and 1000 parts of water.

[0018] The present invention also provides a method for preparing the above-mentioned composition for preventing and treating piglet coccidiosis, comprising the following preparation steps: 1) Weigh the dried medicinal materials pomegranate, verbena, liquorice, sophora flavescens, and coptis chinensis by weight, grind them, and pass them through an 80-mesh sieve. Add 10-30 times the total weight of the medicinal materials in room temperature water, boil for 30 minutes, and filter to obtain a medicinal solution. 2) After cooling the medicinal solution obtained in step 1), the SXDYYB7 microbial preparation is added to obtain the traditional Chinese medicine composition.

[0019] Furthermore, the bacterial count of the SXDYYB7 microbial preparation is greater than or equal to 1.80×10 9 CFU / mL.

[0020] Further preferably, the bacterial count of the SXDYYB7 microbial preparation is 1.80×10 9-10 CFU / mL.

[0021] The beneficial effects of the present invention are as follows: the composition for treating piglet coccidiosis is prepared by boiling pomegranate, verbena, liquorice, sophora flavescens and coptis root in water at normal pressure to obtain a medicinal liquid, and then adding the SXDYYB7 microbial preparation; the probiotics inhibit the reproduction of coccidia by biological oxygen deprivation; the composition can treat and prevent piglet coccidiosis; two weeks before farrowing, the composition is mixed with feed and fed to sows to play a preventive role; after birth, before coccidia appear, the composition is mixed with feed and fed to piglets to prevent piglet coccidiosis, and the cure rate after onset is high.

[0022] Biological Deposit Description Biological material: SXDYYB7, classification name: Bacillus subtilis ( Bacillus subtilis ), deposited in the China Center for Type Culture Collection on February 19, 2025. The address of the collection is: Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province; the collection number is CCTCC No: M 2025256. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a micrograph of Bacillus subtilis SXDYYB7 of the present invention. DETAILED DESCRIPTION

[0024] The principles and features of the present invention are described below (in conjunction with the accompanying drawings). The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0025] The present invention discloses a Bacillus subtilis SXDYYB7 and its application in preventing and controlling piglet coccidiosis. Those skilled in the art can refer to the contents of this article and appropriately improve the process parameters for implementation. It should be noted in particular that all similar replacements and modifications are obvious to those skilled in the art and are considered to be included in the present invention. The methods and applications of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0026] It should be understood that the expression "one or more of" includes individually each of the items recited after the expression and various combinations of two or more of the recited items, unless otherwise apparent from the context and usage. The expression "and / or" in conjunction with three or more recited items should be understood to have the same meaning, unless otherwise apparent from the context.

[0027] The terms "comprising", "having" or "containing", including their grammatical synonyms, should generally be understood as open and non-restrictive, e.g., not excluding other unrecited elements or steps, unless otherwise specifically stated or understood from the context.

[0028] It should be understood that the order of steps or the order in which certain actions are performed is not important as long as the application remains operable. Additionally, two or more steps or actions may be performed simultaneously.

[0029] The use of any and all examples or exemplary language such as "for example" or "including" herein is intended only to better illustrate the present application and does not limit the scope of the present application. No language in this specification should be construed as indicating any non-claimed element is essential to the practice of the present application.

[0030] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0031] The sources of some raw materials of the present invention are as follows: The present invention provides a Bacillus subtilis ( Bacillus subtilis )SXDYYB7, the deposit number is CCTCCNO:M2025256.

[0032] The present invention also provides the above-mentioned Bacillus subtilis ( Bacillus subtilis ) Application of SXDYYB7 in the prevention and treatment of piglet coccidiosis.

[0033] As an optional embodiment, the application includes using the Bacillus subtilis ( Bacillus subtle )SXDYYB7 and / or its metabolites.

[0034] The present invention also provides a composition for preventing and treating piglet coccidiosis, wherein the composition comprises the Bacillus subtilis ( Bacillus subtilis )SXDYYB7 microbial preparation made from SXDYYB7.

[0035] As an optional embodiment, the composition is composed of the following dry medicinal raw materials or dry medicinal raw materials: pomegranate, verbena, liquorice, sophora flavescens, coptis chinensis and SXDYYB7 microbial preparation.

[0036] The SXDYYB7 microbial preparation directly and competitively inhibits the colonization of pathogenic bacteria and secretes antimicrobial peptides. At the same time, it works together with the Chinese medicinal ingredients of pomegranate, verbena, licorice, sophora flavescens, and coptis chinensis to regulate the balance of intestinal microecology.

[0037] As an optional embodiment, the composition is composed of the following dry medicinal materials in parts by weight: 20-30 parts of pomegranate, 2-3 parts of verbena, 10-20 parts of licorice, 20-30 parts of sophora flavescens, 10-30 parts of coptis chinensis, and 1-3 parts of SXDYYB7 microbial preparation.

[0038] As an optional embodiment, the preparation method of the SXDYYB7 microbial preparation is: the Bacillus subtilis ( Bacillus subtilis ) SXDYYB7 seed liquid is inoculated into a fermentation medium, and after continuous culture at 31-35°C for 24-72 hours, it is statically cultured for 96-120 hours to obtain a fermentation liquid; the fermentation liquid is centrifuged to collect the bacteria, and after freeze-drying, the SXDYYB7 microbial preparation is obtained.

[0039] Specifically, the fermentation broth was centrifuged at 4°C and 8000 rpm (r / min) for 15 minutes, the bacterial solution was collected, sterilized skimmed milk powder was used as a freeze-drying protective agent, the bacterial solution was pre-cooled at -20°C for 24 hours, and then placed in a vacuum freeze dryer at -50°C to a moisture content of 3-5%, thereby obtaining the SXDYYB7 microbial preparation.

[0040] As an optional embodiment, the fermentation medium is composed of the following components in parts by weight: 1-5 parts of fish meal, 1-3 parts of corn flour, 1-10 parts of yeast extract, 5-15 parts of sodium chloride, 10-100 parts of soybean meal powder, and 1000 parts of water.

[0041] Further preferably, the fermentation medium consists of the following components in parts by weight: 3 parts of fish meal, 2 parts of corn flour, 5 parts of yeast extract, 10 parts of sodium chloride, 50 parts of soybean meal powder, and 1000 parts of water.

[0042] The present invention also provides a method for preparing the above-mentioned composition for preventing and treating piglet coccidiosis, comprising the following preparation steps: 1) Weigh the dried medicinal materials pomegranate, verbena, liquorice, sophora flavescens, and coptis chinensis by weight, grind them, and pass them through an 80-mesh sieve. Add 10-30 times the total weight of the medicinal materials in room temperature water and boil for 30 minutes. Filter to obtain a medicinal solution. 2) After cooling the medicinal solution obtained in step 1), the SXDYYB7 microbial preparation is added to obtain the traditional Chinese medicine composition.

[0043] As an optional embodiment, the bacterial count of the SXDYYB7 microbial preparation is greater than or equal to 1.80×109 CFU / mL.

[0044] Further preferably, the bacterial count of the SXDYYB7 microbial preparation is 1.80×10 9-10 CFU / mL.

[0045] The present invention is described in detail below in conjunction with embodiments and experimental data.

[0046] Culture medium formula: Fermentation medium: fish meal 3 g / L, corn flour 2 g / L, yeast extract 5 g / L, sodium chloride 10 g / L, soybean meal 50 g / L.

[0047] Example 1 (1) Isolation and screening of strains: The probiotic strain SXDYYB7 of the present invention was isolated from the intestine of healthy piglets and was identified as Bacillus subtilis ( Bacillus subtilis ), specifically, the SXDYYB7 strain was isolated from a naturally recovered sick piglet, heated at 95°C for 10 minutes, and then diluted in multiple proportions and plated to pick out a single colony.

[0048] (2) Strain preservation: The SXDYYB7 strain was deposited in the China Center for Type Culture Collection (CCTCC, address: Wuhan University, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province, Postal Code: 430072) on February 19, 2025, and was classified and named Bacillus subtilis ( Bacillus subtilis ), the deposit number is CCTCC No: M 2025256.

[0049] Example 2 The composition for preventing and treating piglet coccidiosis in this embodiment is prepared from the following dry medicinal materials in parts by weight: 20 parts of pomegranate, 2 parts of verbena, 10 parts of liquorice, 20 parts of sophora flavescens, 10 parts of coptis chinensis, and 1 part of SXDYYB7 microbial preparation.

[0050] The preparation method of the probiotic fermentation Chinese medicine composition for preventing and treating piglet coccidiosis of this embodiment comprises the following steps: 1) Weigh the dried herbs pomegranate, verbena, liquorice, sophora flavescens, and coptis chinensis by weight, crush (pass through an 80-mesh sieve), add 30 times the total weight of the herbs in room temperature water, and boil for 30 minutes; 2) After cooling the medicinal solution obtained in step 1), the SXDYYB7 microbial preparation is added to obtain the traditional Chinese medicine composition.

[0051] The SXDYYB7 microbial preparation is prepared by inoculating SXDYYB7 seed liquid into a culture medium for fermentation; the fermentation conditions are: continuous culture at 32°C for 48 hours, followed by static culture for 96 hours to obtain a fermentation liquid; the fermentation liquid is centrifuged at 4°C and 8000 rpm (r / min) for 15 minutes, the bacterial liquid is collected, sterilized skim milk powder is used as a freeze-drying protective agent, the bacterial liquid is pre-cooled at -20°C for 24 hours, and then placed in a vacuum freeze dryer at -50°C to a moisture content of 3-5%, thereby obtaining a SXDYYB7 microbial preparation containing more than 2×10 9CFU / mL; the culture medium consists of the following components by weight: 3 parts of fish meal, 2 parts of corn flour, 7 parts of yeast extract, 11 parts of sodium chloride, 75 parts of soybean meal powder, and 1000 parts of water.

[0052] Example 3 The composition for preventing and treating piglet coccidiosis in this embodiment is prepared from the following dry medicinal materials in parts by weight: 25 parts of pomegranate, 3 parts of verbena, 15 parts of liquorice, 26 parts of sophora flavescens, 18 parts of coptis chinensis, and 2 parts of SXDYYB7 microbial preparation.

[0053] The preparation method of the composition for preventing and treating piglet coccidiosis of this embodiment comprises the following steps: 1) Weigh the dried herbs pomegranate, verbena, liquorice, sophora flavescens, and coptis chinensis by weight, crush (pass through an 80-mesh sieve), add 30 times the total weight of the herbs in room temperature water, and boil for 30 minutes; 2) After cooling the medicinal solution obtained in step 1), the SXDYYB7 microbial preparation is added to obtain the traditional Chinese medicine composition.

[0054] The SXDYYB7 microbial preparation is prepared by inoculating SXDYYB7 seed liquid into a culture medium for fermentation; the fermentation conditions are: continuous culture at 32°C for 48 hours, followed by static culture for 96 hours to obtain a fermentation liquid; the fermentation liquid is centrifuged at 4°C and 8000 rpm (r / min) for 15 minutes, the bacterial liquid is collected, sterilized skim milk powder is used as a freeze-drying protective agent, the bacterial liquid is pre-cooled at -20°C for 24 hours, and then placed in a vacuum freeze dryer at -50°C to a moisture content of 3-5%, thereby obtaining a SXDYYB7 microbial preparation containing more than 2×10 9 CFU / mL; the culture medium consists of the following components by weight: 3 parts of fish meal, 2 parts of corn flour, 7 parts of yeast extract, 11 parts of sodium chloride, 75 parts of soybean meal powder, and 1000 parts of water.

[0055] Example 4 The composition for preventing and treating piglet coccidiosis in this embodiment is prepared from the following dry medicinal materials in parts by weight: 30 parts of pomegranate, 3 parts of verbena, 20 parts of liquorice, 30 parts of sophora flavescens, 30 parts of coptis chinensis, and 3 parts of SXDYYB7 microbial preparation.

[0056] The preparation method of the composition for preventing and treating piglet coccidiosis of this embodiment comprises the following steps: 1) Weigh the dried herbs pomegranate, verbena, liquorice, sophora flavescens, and coptis chinensis by weight, crush (pass through an 80-mesh sieve), add 30 times the total weight of the herbs in room temperature water, and boil for 30 minutes; 2) After cooling the medicinal solution obtained in step 1), the SXDYYB7 microbial preparation is added to obtain the traditional Chinese medicine composition.

[0057] The SXDYYB7 microbial preparation is prepared by inoculating SXDYYB7 seed liquid into a culture medium for fermentation; the fermentation conditions are: continuous culture at 32°C for 48 hours, followed by static culture for 96 hours to obtain a fermentation liquid; the fermentation liquid is centrifuged at 4°C and 8000 rpm (r / min) for 15 minutes, the bacterial liquid is collected, sterilized skim milk powder is used as a freeze-drying protective agent, the bacterial liquid is pre-cooled at -20°C for 24 hours, and then placed in a vacuum freeze dryer at -50°C to a moisture content of 3-5%, thereby obtaining a SXDYYB7 microbial preparation containing more than 2×10 9 CFU / mL; the culture medium consists of the following components by weight: 3 parts of fish meal, 2 parts of corn flour, 7 parts of yeast extract, 11 parts of sodium chloride, 75 parts of soybean meal powder, and 1000 parts of water.

[0058] Comparative Example 1 The composition for preventing and treating piglet coccidiosis in this comparative example is prepared from the following dry medicinal materials in parts by weight: 3 parts of verbena, 15 parts of liquorice, 26 parts of sophora flavescens, 18 parts of coptis chinensis, and 2 parts of SXDYYB7 microbial preparation.

[0059] The preparation method of the composition for preventing and treating piglet coccidiosis in this comparative example comprises the following steps: 1) Weigh the dried herbs pomegranate, liquorice, sophora flavescens, and coptis chinensis by weight, crush (pass through an 80-mesh sieve), add 30 times the total weight of the herbs in room temperature water, and boil for 30 minutes; 2) After cooling the medicinal solution obtained in step 1), the SXDYYB7 microbial preparation is added to obtain the traditional Chinese medicine composition.

[0060] The SXDYYB7 microbial preparation is prepared by inoculating SXDYYB7 seed liquid into a culture medium for fermentation; the fermentation conditions are: continuous culture at 32°C for 48 hours, followed by static culture for 96 hours to obtain a fermentation liquid; the fermentation liquid is centrifuged at 4°C and 8000 rpm (r / min) for 15 minutes, the bacterial liquid is collected, sterilized skim milk powder is used as a freeze-drying protective agent, the bacterial liquid is pre-cooled at -20°C for 24 hours, and then placed in a vacuum freeze dryer at -50°C to a moisture content of 3-5%, thereby obtaining a SXDYYB7 microbial preparation containing more than 2×10 9 CFU / mL; the culture medium consists of the following components by weight: 3 parts of fish meal, 2 parts of corn flour, 7 parts of yeast extract, 11 parts of sodium chloride, 75 parts of soybean meal powder, and 1000 parts of water.

[0061] Comparative Example 2 Compared with Example 3, the only difference is that the fermentation conditions are: continuous culture at 30° C. for 48 hours and then static culture for 96 hours.

[0062] Comparative Example 3 Compared with Example 3, the only difference is that the fermentation medium is: 5 parts of fish meal, 4 parts of yeast extract, 10 parts of sodium chloride, 50 parts of soybean meal powder, and 1000 parts of water.

[0063] Comparative Example 4 Compared with Example 3, the only difference is that no SXDYYB7 microbial preparation is added.

[0064] Comparative Example 5 Compared with Example 3, the difference is that only the SXDYYB7 microbial preparation is contained.

[0065] Comparative Example 6 Compared with Example 3, the only difference is that commercially available probiotics (Bacillus subtilis) are used instead of the SXDYYB7 microbial preparation.

[0066] The following experimental examples illustrate the beneficial effects of the present invention. Experimental methods in the following experimental examples where specific conditions are not specified are generally measured in accordance with national standards. If no corresponding national standards exist, the methods are conducted in accordance with commonly used international standards, conventional conditions, or conditions recommended by the manufacturer.

[0067] Experimental Example 1: Treatment Effect Experiment Experimental animals: Piglets diagnosed with coccidiosis.

[0068] The piglets were divided into an infection group (piglets suffering from coccidiosis, not treated) and an experimental group (piglets suffering from coccidiosis, treated). The experimental groups were treated with the compositions of Examples 2-4 and Comparative Examples 1-6, respectively. Each piglet was fed 100 g of the composition mixed with 1000 g of feed per meal for 3 consecutive days, with an interval of 7 days before starting another course of treatment for 3 consecutive courses.

[0069] Table 1 Treatment effect results

[0070] As can be seen from Table 1, the compositions of Examples 2-4 have a high cure rate for sick piglets, proving that the SXDYYB7 microbial system of the present invention works synergistically with pomegranate, verbena, licorice, sophora flavescens, and coptis chinensis to achieve a better therapeutic effect. It can be seen that the fermentation conditions of Comparative Example 2 are different, and the culture medium components of Comparative Example 3 are different, and the cure rates of both are not as high as those of Examples 2-4.

[0071] Experimental Example 2: Preventive Effect Experiment Prevention of sows about to parturite: The sows about to parturite were divided into a blank group (no prevention) and a prevention group (prevention). The prevention groups were respectively treated with the compositions of Examples 2-4 and Comparative Examples 1-6. The sows about to parturite were fed with 100 g of the composition mixed with 3000 g of feed per meal for 3 days.

[0072] Prevention of unmarketed piglets: Unmarketed piglets were divided into a blank group (no prevention) and a prevention group (prevention). The prevention groups were treated with the compositions of Examples 2-4 and Comparative Examples 1-6, respectively, at a rate of 20 grams per piglet mixed into feed for two consecutive days. The prevention effect results are shown in Table 2 below: Table 2 Prevention effect results

[0073] As can be seen from Table 2, the SXDYYB7 microbial system of the present invention cooperates with pomegranate, verbena, liquorice, sophora flavescens, and coptis chinensis to achieve a better preventive effect.

[0074] In summary, the composition for treating piglet coccidiosis of the present invention is prepared by boiling pomegranate, verbena, licorice, sophora flavescens, and coptis chinensis in water at normal pressure to obtain a medicinal liquid, and then adding the SXDYYB7 microbial preparation. The probiotics inhibit the reproduction of coccidia by biological oxygen deprivation. The composition of the present invention can treat and prevent piglet coccidiosis. Two weeks before the sow gives birth, the composition is mixed with feed and fed to the sow to play a preventive role. After the piglet is born, before the coccidia appear, the composition is mixed with feed and fed to the piglet to prevent piglet coccidiosis, and the cure rate after the onset of the disease is high.

[0075] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A Bacillus subtilis ( Bacillus subtilis )SXDYYB7, characterized in that, The accession number is CCTCCNO:M 2025256.

2. A Bacillus subtilis according to claim 1 ( Bacillus subtilis ) Application of SXDYYB7 in the prevention and treatment of piglet coccidiosis.

3. The use according to claim 2, characterized in that The application includes using the SXDYYB7 and / or its metabolites.

4. A composition for preventing and treating coccidiosis in piglets, characterized in that: The composition comprises the Bacillus subtilis according to claim 1 ( Bacillus subtilis )SXDYYB7 microbial preparation made from SXDYYB7.

5. A composition for preventing and treating piglet coccidiosis according to claim 4, characterized in that, The composition is composed of the following dry medicinal raw materials: pomegranate, verbena, liquorice, sophora flavescens, coptis chinensis, and SXDYYB7 microbial preparation.

6. A composition for preventing and treating piglet coccidiosis according to claim 4, characterized in that: The composition is composed of the following dry medicinal materials in parts by weight: 20-30 parts of pomegranate, 2-3 parts of verbena, 10-20 parts of licorice, 20-30 parts of sophora flavescens, 10-30 parts of coptis chinensis, and 1-3 parts of SXDYYB7 microbial preparation.

7. A composition for preventing and treating piglet coccidiosis according to claim 5, characterized in that: The preparation method of the SXDYYB7 microbial preparation is as follows: Bacillus subtilis ) SXDYYB7 seed liquid is inoculated into a fermentation medium, and after continuous culture at 31-35°C for 24-48 hours, it is statically cultured for 96-120 hours to obtain a fermentation liquid; the fermentation liquid is centrifuged to collect the bacteria, and after freeze-drying, the SXDYYB7 microbial preparation is obtained.

8. A composition for preventing and treating piglet coccidiosis according to claim 7, characterized in that: The fermentation medium consists of the following components in parts by weight: 1-5 parts of fish meal, 1-3 parts of corn flour, 1-10 parts of yeast extract, 5-15 parts of sodium chloride, 10-100 parts of soybean meal powder, and 1000 parts of water.

9. The method for preparing the composition for preventing and treating piglet coccidia according to any one of claims 5 to 8, wherein: The method comprises the following preparation steps: 1) Weigh the dried medicinal materials pomegranate, verbena, liquorice, sophora flavescens, and coptis chinensis by weight, grind them, and pass through an 80-mesh sieve. Add 10-30 times the total weight of the medicinal materials in room temperature water, boil for 30 minutes, and filter to obtain a medicinal solution. 2) After cooling the medicinal solution obtained in step 1), the SXDYYB7 microbial preparation is added to obtain the traditional Chinese medicine composition.

10. The method for preparing the composition for preventing and treating coccidia in piglets according to claim 9, wherein: The bacterial count of the SXDYYB7 microbial preparation is greater than or equal to 1.80×10 9 CFU / mL.

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