Chinese herbal medicine formula and application thereof in laying fowl feed

Abstract

The invention provides a Chinese herbal medicine formula and application thereof in laying poultry feed. The Chinese herbal medicine formula comprises the following components in parts by weight: 15-40 parts of radix acanthopanacis semticosi, 15-30 parts of radix puerariae, 10-30 parts of radix paeoniae alba, 3-10 parts of schisandra chinensis, 5-12 parts of pericarpium citri reticulatae, 3-10 parts of rhizoma atractylodis macrocephalae, 3-15 parts of leonurus, 1-8 parts of polygala tenuifolia and 1-7 parts of ginseng leaves. The Chinese herbal medicine formula can significantly improve the laying rate and the yolk specific gravity of laying hens, reduce the feed-egg ratio, improve the reproductive hormone level of the laying hens to promote egg laying, also significantly improve the immune function of the laying hens, has no side effect on the liver and kidney functions of the laying hens, and can significantly improve the production performance of the laying hens, reduce the feed-egg ratio and increase the breeding benefits of the laying hens after being applied.

Description

Technical Field

[0001] The invention relates to the technical field of laying fowl breeding, and in particular to a Chinese herbal medicine formula and application thereof in laying fowl feed. Background Art

[0002] my country's Ministry of Agriculture and Rural Affairs has issued a notice strictly restricting the use of antibiotics in egg-laying poultry farming starting in 2020. As antibiotics gradually phase out, the feed industry officially enters an "antibiotic-free era." This urges us to find alternatives to antibiotic feed additives to meet the public's demand for high-quality, healthy animal-based food.

[0003] Chinese herbal medicine is derived from nature and has the advantages of wide sources, low toxicity, no drug resistance, etc. Compared with other antibiotic substitutes such as single plant extracts, the compound Chinese herbal medicine additives created under the guidance of Chinese veterinary medicine theory have multiple targets, multiple effects, and obvious advantages such as high efficiency, safety, and no residue. Chinese herbal medicine feed additives have become a research hotspot in today's egg-laying poultry farming industry. The main ingredients of the Chinese herbal medicine additives currently used for egg-laying poultry farming are common epimedium, eucommia bark, angelica sinensis, astragalus root, etc. The price of medicinal raw materials is relatively high, and the existing commodity ingredients are similar, with low innovation, few product types, and high cost of use. The present invention is based on the reproductive physiological characteristics of laying hens, combined with the guidance of Chinese veterinary medicine theory, with tonifying the spleen and replenishing qi, protecting the liver and kidney, replenishing blood and tranquilizing the mind, and nourishing the uterus as the prescription principle, selecting the Chinese herbal medicine ingredients with definite efficacy, low price and good quality, and the same origin of medicine and food to be compounded, and finally determining that 9 herbs such as ginseng leaf, acanthopanax senticosus root, dried tangerine peel, white peony root form the compound Chinese herbal medicine formula, i.e. "good eggs more". Summary of the Invention

[0004] The purpose of the present invention is to provide a Chinese herbal medicine formula and its application in laying fowl feed to significantly improve the production performance of laying hens. In order to achieve this technical purpose, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a Chinese herbal medicine formula, which comprises, by weight, 15 to 40 parts of Acanthopanax senticosus root, 15 to 30 parts of Pueraria root, 10 to 30 parts of White Peony Root, 3 to 10 parts of Schisandra chinensis, 5 to 12 parts of Tangerine Peel, 3 to 10 parts of Atractylodes macrocephala, 3 to 15 parts of Leonurus japonicus, 1 to 8 parts of Polygala tenuifolia, and 1 to 7 parts of Panax ginseng leaf.

[0005] Preferably, the Chinese herbal medicine formula comprises, by weight: 25-35 parts of Acanthopanax senticosus root, 20-30 parts of Pueraria root, 13-20 parts of White Peony Root, 5-10 parts of Schisandra chinensis, 5-10 parts of Tangerine Peel, 5-10 parts of Atractylodes macrocephala, 3-10 parts of Leonurus japonicus, 1-5 parts of Polygala tenuifolia, and 1-5 parts of Ginseng Leaf.

[0006] Preferably, the Chinese herbal medicine formula comprises, by weight: 28-30 parts of Acanthopanax senticosus root, 20-22 parts of Pueraria root, 15-16 parts of White Peony Root, 6-7 parts of Schisandra chinensis, 6-7 parts of Tangerine Peel, 6-7 parts of Atractylodes macrocephala, 5-6 parts of Leonurus japonicus, 2-3 parts of Polygala tenuifolia, and 2-3 parts of Ginseng Leaf.

[0007] In a second aspect, the present invention provides use of the Chinese herbal medicine formula described in the first aspect in preparing an additive for laying fowl feed.

[0008] In a third aspect, the present invention provides a laying fowl feed additive comprising the Chinese herbal medicine formula described in the first aspect.

[0009] In a fourth aspect, the present invention provides a laying fowl feed, comprising: a basic diet and the Chinese herbal medicine formula described in the first aspect.

[0010] Preferably, the added amount of the Chinese herbal medicine formula is 0.5-1% of the mass of the basic diet.

[0011] Preferably, the laying birds include chickens, ducks, geese, and quails.

[0012] In a fifth aspect, the present invention provides a method for improving the production performance of laying fowls, comprising: feeding the laying fowls the Chinese herbal medicine formula described in the first aspect twice a day.

[0013] Preferably, the Chinese herbal formula is mixed with a basal diet and fed to the pigs, and the added amount of the Chinese herbal formula is 0.5-1% of the mass of the basal diet, more preferably 0.7-1%.

[0014] The present invention's herbal feed additive is formulated under the guidance of traditional Chinese veterinary medicine theory, with the principles of tonifying the spleen and replenishing qi, protecting the liver and benefiting the kidneys, nourishing the blood and calming the mind, and nourishing the uterus. Nine herbs, including ginseng leaf, Acanthopanax senticosus root, dried tangerine peel, and white peony root, are used to form a herbal compound. Acanthopanax senticosus has a sweet, bitter, pungent, and warm nature, and has the effects of tonifying qi and strengthening the spleen, nourishing the kidneys, and calming the mind. Pueraria root has a sweet, pungent, and cooling nature, and enters the spleen, stomach, and lung meridians. It exerts the effects of clearing heat and promoting fluid production, strengthening the spleen and benefiting the stomach, and promoting yang and stopping diarrhea. White peony root is sweet, pungent, and warm in nature; it warms yang and dispels dampness, replenishes body deficiency, nourishes blood and regulates menstruation, and strengthens the spleen and stomach. It complements Acanthopanax senticosus root and white peony root to tonify both qi and blood. Schisandra chinensis has astringent and firming properties, replenishes qi and promotes fluid production, and nourishes the kidneys and calms the mind. Tangerine peel has a pungent, dispersing, bitter, and drying nature, and has the effects of regulating qi and strengthening the spleen, drying dampness and resolving phlegm. Atractylodes macrocephala is bitter, sweet, and warm in nature, and has the effects of tonifying the spleen and replenishing qi, drying dampness and promoting diuresis, stopping sweating, and stabilizing the fetus. Motherwort promotes blood circulation, removes blood stasis, regulates menstruation, and eliminates water retention. Its active ingredient, leonurin, is used internally to dilate blood vessels and lower blood pressure. It also acts as an antagonist to adrenaline, increasing the frequency of uterine movements and promoting contractions. Polygala tenuifolia (Polygala tenuifolia) is bitter, pungent, and warm in nature, and enters the heart, kidney, and lung meridians, offering calming, intellectual, and depression-relieving benefits. Ginseng leaf is bitter, sweet, and cold in nature, and enters the lung and stomach meridians, tonifying qi, benefiting the lungs, dispelling heat, and promoting fluid production.

[0015] Experiments have shown that the formula of the present invention, which uses Acanthopanax senticosus root as the monarch herb, Pueraria root and White Peony Root as assistant herbs, Schisandra chinensis, Tangerine peel, Atractylodes macrocephala, and Leonurus japonicus as adjuvant herbs, and Polygala tenuifolia and Panax ginseng leaves as guiding herbs, can significantly improve the production performance of laying hens. The adjuvant herbs synergize with the monarch herb by strengthening the spleen and kidneys, while the assistant herbs have the effects of invigorating Qi, calming the mind, nourishing blood, and regulating menstruation. Polygala tenuifolia and Panax ginseng leaves as guiding herbs can also have a calming effect, helping laying hens resist stress and reduce morbidity and mortality. Furthermore, a formula composed of these nine Chinese herbs (abbreviated as "Haodanduo"), when added to feed at a rate of 0.5% to 1%, can increase the egg production rate of 52-55 week-old Hy-Line Grey laying hens by approximately 7-14%, while reducing the feed-to-egg ratio by approximately 4%. In addition, "Haodanduo" can increase the proportion of egg yolk to varying degrees and improve the flavor of eggs; it has no significant effect on other egg qualities and serum biochemical indicators of laying hens; "Haodanduo" has a significant effect on the levels of reproductive hormones and immune factors of laying hens, and promotes the reproductive performance and body health of laying hens, and can be used to improve the production performance of laying hens. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings: Figure 1 Figure 1 shows the production performance of laying hens in Examples 1-3 and Comparative Examples 1-3 of the present invention. Figure A shows the change in egg production over time, Figure B shows the change in average egg weight over time, Figure C shows the change in average feed intake over time, and Figure D shows the change in feed-to-egg ratio over time. Note: * indicates a significant difference between the two groups, P < 0.05. Same as the following figures.

[0017] Figure 2 Figure 2 shows the effects of different dosages of Haodanduo on the production performance of laying hens in Application Example 2 of the present invention, wherein Figure A shows the changes in egg production rate over time, Figure B shows the changes in average egg weight over time, Figure C shows the changes in average feed intake over time, and Figure D shows the changes in feed-to-egg ratio over time.

[0018] Figure 3 The present invention shows the effect of Haodanduo on egg quality in Application Example 2, wherein Figure A shows the change of egg shape index at 28 days and 56 days, Figure B shows the change of yolk specific gravity at 28 days and 56 days, Figure C shows the change of eggshell thickness at 28 days and 56 days, Figure D shows the change of albumen height at 28 days and 56 days, Figure E shows the change of Haugh unit at 28 days and 56 days, and Figure F shows the change of yolk color at 28 days and 56 days.

[0019] Figure 4This figure shows the effects of Haodanduo on serum biochemical and fat metabolism indicators in Application Example 2 of the present invention, wherein Figures A to P respectively represent the levels of TBA, TC, UA, BUN, GLU, TG, FFA, ALB, TP, CK, ALT, AST, ALP, ADP, VTG, and LEP in each experimental group.

[0020] Figure 5 This is the effect of Haodanduo on reproductive hormones in laying hens in Application Example 2 of the present invention, wherein Figures A to E respectively represent the levels of estradiol, progesterone, prolactin, follicle-stimulating hormone, and luteinizing hormone in each experimental group.

[0021] Figure 6 This is the effect of Haodanduo on the serum immune factors of laying hens in Application Example 2 of the present invention, wherein Figures A to F respectively represent the levels of IL-6, IFN-γ, IL-2, TNF-α, IL-4, and IL-10 in each experimental group. DETAILED DESCRIPTION

[0022] In the examples of the present invention, the Chinese herbal medicine ingredients used were all purchased from a Chinese herbal medicine decoction piece company in Liangshan.

[0023] In the embodiment of the present invention, the instruments and equipment required for the experiment are shown in Table 1 and are provided by Panxi Key Laboratory of Animal Disease Detection and Prevention.

[0024] Table 1 Main instruments and equipment

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] Example 1

[0027] This embodiment provides a Chinese herbal medicine formula, comprising, by weight, 30 parts of white peony root, 22 parts of kudzu root, 15 parts of acanthopanax senticosus, 7 parts of schisandra chinensis, 7 parts of dried tangerine peel, 7 parts of atractylodes macrocephala, 6 parts of motherwort, 3 parts of polygala tenuifolia, and 3 parts of ginseng leaf. Each of the medicinal materials is dried to a constant weight, ultrafinely ground through a 200-mesh sieve, and mixed to obtain the Chinese herbal medicine formula, which is then sealed and stored in a dark, dry place until ready for use.

[0028] Example 2

[0029] This embodiment provides a Chinese herbal medicine formula, which comprises the following ingredients in parts by weight: 30 parts of Pueraria root, 22 parts of Acanthopanax root, 15 parts of White Peony Root, 7 parts of Schisandra chinensis, 7 parts of Tangerine Peel, 7 parts of Atractylodes macrocephala, 6 parts of Leonurus japonicus, 3 parts of Polygala tenuifolia, and 3 parts of Panax ginseng leaf.

[0030] Example 3

[0031] This embodiment provides a Chinese herbal medicine formula, which comprises the following ingredients in parts by weight: 30 parts of Acanthopanax senticosus root, 22 parts of Pueraria root, 15 parts of White Peony Root, 7 parts of Schisandra chinensis, 7 parts of Tangerine Peel, 7 parts of Atractylodes macrocephala, 6 parts of Leonurus japonicus, 3 parts of Polygala tenuifolia, and 3 parts of Panax ginseng leaf.

[0032] Comparative Example 1 This comparative example provides a Chinese herbal medicine formula, which comprises the following ingredients in parts by weight: 33 parts of Codonopsis pilosula leaves, 22 parts of Pueraria root, 15 parts of white peony root, 7 parts of Schisandra chinensis, 7 parts of dried tangerine peel, 7 parts of Atractylodes macrocephala, 6 parts of Leonurus japonicus, and 3 parts of Polygala tenuifolia.

[0033] Comparative Example 2 This comparative example provides a Chinese herbal medicine formula, which comprises the following ingredients in parts by weight: 35 parts of Acanthopanax senticosus roots, 25 parts of White Peony Roots, 7 parts of Schisandrae Chinensis Fruits, 7 parts of Tangerine Peel, 10 parts of Leonurus japonicus, 10 parts of Atractylodes macrocephala, 3 parts of Polygala tenuifolia, and 3 parts of Panax ginseng leaves.

[0034] Comparative Example 3 This comparative example provides a Chinese herbal medicine formula, which comprises the following ingredients in parts by weight: 30 parts of Acanthopanax senticosus roots, 25 parts of Pueraria root, 20 parts of dried tangerine peel, 15 parts of Leonurus japonicus, and 10 parts of ginseng leaves.

[0035] Application Example 1 The Chinese herbal medicine formulas of Examples 1 to 3 and Comparative Examples 1 to 3 were used to test the production performance of Hailan gray laying hens, as follows: 1 Animal grouping and treatment The experiment was conducted at Runsheng Family Farm in Mianning County, Xichang City. 504 healthy, 52-week-old Hy-Line Gray laying hens of similar weight were randomly divided into seven groups: a blank control group, Example 1 (Group A), Example 2 (Group B), Example 3 (Group C), Comparative Example 1 (Group D), Comparative Example 2 (Group E), and Comparative Example 3 (Group F). Each group had six replicates, with 12 hens per replicate. Except for the blank control group, all other groups received a 0.7% supplemental dose of the corresponding traditional Chinese medicine compound. The diet used was Hualing 155, a high-yield laying hen feed from Xichang Rongbo Trading Co., Ltd. (see Table 2 for dietary composition). For a 7-day pre-test period and a 56-day full-scale test period, each group was fed a specific diet.

[0036] The chicken houses were naturally ventilated, maintained at a temperature of 15-28°C, a relative humidity of 50-60%, and a 16-hour light day. During the experiment, the chickens were fed their daily diet at 5:30 AM and 3:00 PM. Eggs were collected and weighed at 6:30 PM. Residual feed was weighed every Sunday at 1:00 PM.

[0037] Table 2 Diet composition and nutritional levels of laying hens

[0038] Table 3 Guaranteed values ​​of laying hen diet composition analysis

[0039] 2 Methods for measuring laying hen production performance During the experimental period, the total number of eggs laid and the total weight of eggs laid for each replicate were recorded daily, and the egg production rate and average egg weight were calculated. The average daily feed intake and feed-to-egg ratio of each group were calculated based on the weekly feed consumption. Egg production rate (%) = (total number of eggs produced per day / total number of laying hens) × 100% Formula (1); Average egg weight (g) = total daily egg weight / total daily egg number formula (2); Daily feed intake (g) = (weekly feed amount - weekly remaining feed amount) / (7 × total number of laying hens) Formula (3); Feed-to-egg ratio = total feed intake during laying period (kg) / total egg weight (kg) Formula (4); 3 Effects of different Chinese herbal formulas on laying hen production performance See the results Figure 1 The results showed that compared with the blank control group, the Chinese herbal formulas of Examples 1-3 all improved the egg production rate of laying hens 1-8 weeks after administration, among which the Chinese herbal formula of Example 3 had the best effect in promoting egg production rate, with the egg production rate increased by 13.81% ( P <0.05); the egg production rates of the other groups showed no significant difference compared with the blank control group ( P >0.05).

[0040] Application Example 2 The Chinese medicine formula of Example 3 (abbreviated as "Haodanduo") was further investigated as follows: 1 Animal grouping and treatment The experiment was conducted at Runsheng Family Farm in Mianning County, Xichang City. 216 healthy, 55-week-old Hy-Line Gray laying hens of similar weight were randomly divided into three groups: a blank control group (CK group, fed only the diet), a high-dose Haodanduo group (H group, fed the diet with 1% Haodanduo), and a low-dose Haodanduo group (L group, fed the diet with 0.5% Haodanduo). Each group had six replicates, with 12 hens per replicate. During the 7-day pre-trial period, all groups were fed the diet. The final trial lasted 56 days, with each group receiving their own diet.

[0041] The chicken coop was a semi-open, naturally ventilated house. The temperature was maintained at 15-28°C, the relative humidity at 50-60%, and the daylight hours were 16 hours per day. During the trial, the chickens were fed their daily diet at 5:30 AM and 3:00 PM. Eggs were collected and weighed at 6:30 PM. Residual feed was weighed every Sunday at 1:00 PM.

[0042] The diet used was Hualing 155, a high-yield laying hen feed produced by Xichang Rongbo Trading Co., Ltd. Its composition, nutritional ingredients, and guaranteed values ​​(%) of feed ingredient analysis were the same as those in Tables 2 and 3 of Application Example 1. The nutritional ingredients and content of Haodanduo are shown in Table 4.

[0043] Table 4 Nutritional composition and content of “Haodanduo”

[0044] 2 Methods 2.1 Production performance measurement (same method as in Application Example 1) During the experimental period, the total number of eggs and the total weight of eggs produced were recorded every day, and the egg production rate and average egg weight were calculated. [4] ; Every Sunday at 18:00, calculate the feed consumption of the week and the average daily feed intake and feed-to-egg ratio of laying hens; Egg production rate (%) = (total number of eggs produced per day / total number of laying hens) × 100% Formula (1); Average egg weight (g) = total daily egg weight / total daily egg number formula (2); Daily feed intake (g) = (weekly feed amount - weekly feed surplus) / (7 × total number of laying hens) Formula (3); Feed-to-egg ratio = total feed intake during laying period (kg) / total egg weight (kg) Formula (5); 2.2 Egg quality determination (same method as in Application Example 1) At the middle (28 days) and end (56 days) of the formal experiment, 24 qualified eggs were randomly selected from each group to test indicators such as egg shape index, eggshell thickness, yolk specific gravity, and Haugh unit.

[0045] Egg-shaped index = major diameter / minor diameter formula (6); Yolk specific gravity = (yolk weight / egg weight) × 100% Formula (7); Haugh unit = 100 × 1g (H-1.7W 0.37 +7.6) where: H is the albumen height (mm); W is the egg weight (g) Formula (8); 2.3 Biochemical index detection After the experiment, two chickens were randomly selected from each replicate. After fasting for 12 hours, sub-wing venous blood was collected into 5 ml coagulant tubes and centrifuged at 3000 rpm for 12 minutes. Serum was collected and stored at 4°C. Total cholesterol (TCH), triglycerides (TG), uric acid (UA), glucose (GLU), total protein (TP), albumin (ALB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were measured using a Mindray biochemical analyzer.

[0046] 2.4 Fat metabolism index detection Serum collected at 1.6 was assayed for adiponectin (ADP), vitellogenin (VTG), and leptin (LEP) levels in each group using ELISA kits purchased from Shanghai ELISA Biotechnology Co., Ltd., and procedures were performed according to the manufacturer's instructions.

[0047] 2.5 Reproductive hormone measurement Elisa was used to detect the levels of reproductive hormones such as progesterone (PROG), estradiol (E2), prolactin (PRL), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in the serum of each group collected on day 1.6. The kits were purchased from Shanghai ELISA Biotechnology Co., Ltd. and the operation was carried out according to the instructions.

[0048] 2.6 Immune factor detection The serum collected in 1.6 was used to detect the levels of interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 10 (IL-10), interferon γ (IFN-γ), and tumor necrosis factor α (TNF-α) in each group using Elisa. The kits were purchased from Shanghai Enzyme-Linked Biotechnology Co., Ltd., and the operation was carried out according to the instructions.

[0049] 2.7 Data Processing and Statistics The experimental data were preliminarily processed using EXCEL2010 software, and SPSS 24.0 statistical software was used for one-way analysis of variance and LSD method for multiple comparisons. The experimental results are expressed as "mean ± standard error". P＜0.05 Indicates significant difference.

[0050] 3 Results 3.1 Effects of “High-quality Eggs” on the Production Performance of Layers See the results Figure 2 (AD). On the 28th day of the experiment, there were no significant differences in egg production rate, average egg weight, average daily feed intake, feed-to-egg ratio, etc. among the groups ( P>0.05 ), but the egg production rates of the L and H groups were 6.16% higher than those of the control group ( P>0.05 ) and 8.68% ( P>0.05 ); On day 56 of the experiment, there were no significant differences in average egg weight, average daily feed intake, feed-to-egg ratio, etc. among the groups ( P>0.05 ), but compared with the control group, the egg production rates of group L and group H increased by 7.39% ( P>0.05 ) and 12.42% ( P＜0.05 ), while the feed-to-egg ratio decreased by 3.96% ( P>0.05 ).

[0051] 3.2 The impact of “more good eggs” on egg quality See the results Figure 3 On the 28th day of the experiment, the yolk specific gravity of the L and H groups increased by 17.97% ( P< 0.05 )、16.59%( P>0.05 There were no significant differences in other indicators among the groups ( P>0.05 On the 56th day of the experiment, compared with the CK group, the protein height and Haugh unit of the H group decreased by 11.65% ( P<0.05 )、5.62%( P<0.05 ), there were no significant differences in other indicators.

[0052] 3.3 Effects of “Good Eggs” on serum biochemical and fat metabolism indices in laying hens Depend on Figure 4 It can be seen that on the 56th day of the experiment, compared with the CK group, adding different doses of "Haodanduo" to the diet had no significant effect on the serum biochemical indicators of laying hens, including total bile acid (TBA), total cholesterol (TC), uric acid (UA, urea nitrogen (BUN, glucose (GLU, triglyceride (TG), free fatty acid (FFA), albumin (ALB), total protein (TP), creatine kinase (CK), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). P>0.05 ).

[0053] Both 0.5% and 1% of "Haodanduo" significantly increased the serum levels of adiponectin (ADP) and vitellogenin (VTG) (P<0.05), and decreased the level of leptin (LEP) (P<0.05); the effect of group H was the most obvious. Compared with group CK, ADP and VTG increased by 30.08% (P<0.05) and 47.37% (P<0.05), respectively, and LEP decreased by 25.29% (P<0.05).

[0054] 3.4 Effects of Haodanduo on Reproductive Hormones in Laying Hens Depend on Figure 5 It can be seen that compared with the CK group, adding 0.5% and 1% of "Haodanduo" to the diet for 56 days significantly increased the E2, PROG, PRL, and LH levels in the serum of laying hens (P<0.05), but significantly inhibited the FSH level (P<0.05); among them, the addition of 1% Haodanduo had the best effect, increasing serum E2, PROG, PRL, and LH by 42.99% (P<0.05), 51.00% (P<0.05), 32.25% (P<0.05), and 35.73% (P<0.05), respectively, and reducing FSH by 21.72% (P<0.05). 3.5 Effect of "Haodanduo" on serum immune indices of laying hens Depend on Figure 6 Compared with the blank group, the addition of 0.5% and 1% of "Haodanduo" to the diet significantly increased serum IL-6, IL-2, IFN-γ, and TNF-α levels (P<0.05), and decreased IL-4 and IL-10 levels (P<0.05). The 1% addition had the best effect, increasing IL-6, IL-2, IFN-γ, and TNF-α by 40.58% (P<0.05), 40.03% (P<0.05), 30.30% (P<0.05), and 45.02% (P<0.05), respectively, while decreasing IL-4 and IL-10 by 27.77% (P<0.05) and 22.97% (P<0.05), respectively.

[0055] 3.6 Economic Benefit Analysis after Application of “Haodanduo” From November 2024 to January 2025, local eggs sold for approximately 5.25 yuan / kg on the farm, and the market price of feed was 3.85 yuan / kg. The cost of Haodanduo was 23.75 yuan / kg. The results of Application Example 2 show that when 1% Haodanduo was added to the diets of 72 laying hens, the number of eggs produced and the weight of the eggs, as well as the feed intake and the amount of Chinese medicine added, were increased to 56 days old. (See Table 5 for details.)

[0056] Table 5 Comparison of the effects of using “Haodanduo” on promoting egg production in laying hens

[0057] From the table above, we can see that 1% of "Haodanduo" was added to the diet of 72 laying hens for 56 consecutive days. Compared with the blank control group, (1) the total number of eggs increased by 340, the egg weight increased by 23.47 kg, and the egg sales increased by 123.22 yuan; (2) 4.49 kg of feed was saved, totaling 17.2865 yuan; (3) the cost of adding "Haodanduo" was 121.13 yuan.

[0058] Profit is calculated according to the following formula: Profit = Increased egg sales after using Haodanduo (RMB) + Saved feed costs (RMB) - Haodanduo cost (RMB) = 123.22 + 17.29 - 121.13 = 19.38 (RMB) That is, if 55-week-old laying hens use 1% of "Haodanduo" for 56 days, the average profit of each laying hen can be increased by 0.27 yuan.

[0059] 4 Conclusion The addition of "Haodanduo" in feed at 0.5% and 1% can increase the egg production rate of 54-week-old Hy-Line grey laying hens by 7.39% and 1.0% respectively after 56 days of use. P>0.05 ) and 12.42% ( P＜0.05 ), while the feed-to-egg ratio decreased by 3.96% ( P>0.05 Compared with the CK group, the addition of 0.5% and 1% of "Haodanduo" to the diet increased the yolk proportion by 17.97% on the 28th day ( P< 0.05 )、16.59%( P>0.05 ); added to the 56th day, the yolk proportion increased by 1.64% ( P>0.05 )、5.5%( P> 0.05 Combined with the experimental results of Application Example 1, the addition of "Haodanduo" in the feed was 0.7%, and the egg production rate increased by 13.81% compared with the blank control group ( P <0.05), it can be seen that the addition amount of "Haodanduo" in feed should be controlled at 1% or below, preferably 0.7-1%. Continuing to increase the dosage may cause the application cost of "Haodanduo" to be higher than the benefits it brings.

[0060] "Haodanduo" has no significant effect on the serum biochemical parameters of laying hens, indicating that it has no side effects on the liver and kidney function and nutrient metabolism of laying hens. Studies have shown that egg yolk is mainly derived from yolk precursors synthesized by the liver under the action of estrogen and transported to the developing follicles through the blood circulation.

[21] The results of Application Example 2 above show that adding "Haodanduo" to the diet can significantly increase the serum levels of adiponectin and vitellogenin, promote the formation of egg yolk, and increase the specific gravity of egg yolk.

[0061] "Haodanduo" has the effects of replenishing Qi and blood, and benefiting the liver and kidneys. It can significantly increase the secretion of estradiol, progesterone, prolactin, and luteinizing hormone in the serum of laying hens, and has the effect of promoting egg maturation and ovulation. Therefore, adding 0.5% and 1% of Haodanduo to the laying hen diet can increase egg production to varying degrees.

[0062] "Haodanduo" can significantly improve the immune function of laying hens by increasing the pro-inflammatory factors IL-6, IL-2, IFN-γ and TNF-α in the serum of laying hens.

[0063] In addition, Application Examples 1 and 2 of the present invention were both tested using laying hens aged 50+ weeks. Laying hens of this age have reached the late stage of their laying cycle, and their egg production rate has decreased significantly. Through this investigation, it can be seen that the traditional Chinese medicine formula of the present invention can further improve the egg production rate of laying hens in this cycle, and has obvious economic benefits.

[0064] To sum up, "Haodanduo" can not only significantly improve the egg production rate and egg yolk proportion of laying hens, increase the reproductive hormone level of laying hens and promote egg laying, but also significantly improve the immune function of laying hens, and has no side effects on the liver and kidney function of laying hens. After application, it can significantly improve the production performance of laying hens, reduce the feed-to-egg ratio, and increase the efficiency of laying hen farming.

[0065] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents. The embodiments described above merely illustrate several implementation methods of the present invention, and their descriptions are relatively specific and detailed, but they cannot be construed as limiting the scope of the patent of the present invention. It should be pointed out that, without departing from the concept of the present invention, a number of variations and improvements may be made by those skilled in the art, and these all fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent of the present invention shall be based on the appended claims.

Claims

1. A Chinese herbal medicine formula, characterized in that: The Chinese herbal medicine formula comprises, by weight, 15-40 parts of Acanthopanax senticosus roots, 15-30 parts of Pueraria root, 10-30 parts of White Peony Root, 3-10 parts of Schisandra chinensis, 5-12 parts of Tangerine Peel, 3-10 parts of Atractylodes macrocephala, 3-15 parts of Leonurus japonicus, 1-8 parts of Polygala tenuifolia, and 1-7 parts of Panax ginseng leaves.

2. The Chinese herbal medicine formula according to claim 1, characterized in that: The Chinese herbal medicine formula comprises, by weight, 25-35 parts of Acanthopanax senticosus roots, 20-30 parts of Pueraria root, 13-20 parts of White Peony Root, 5-10 parts of Schisandra chinensis, 5-10 parts of Tangerine Peel, 5-10 parts of Atractylodes macrocephala, 3-10 parts of Leonurus japonicus, 1-5 parts of Polygala tenuifolia, and 1-5 parts of Panax ginseng leaf.

3. The Chinese herbal medicine formula according to claim 2, characterized in that: The Chinese herbal medicine formula comprises, by weight, 28-30 parts of Acanthopanax senticosus roots, 20-22 parts of Pueraria root, 15-16 parts of White Peony Root, 6-7 parts of Schisandra chinensis, 6-7 parts of Tangerine Peel, 6-7 parts of Atractylodes macrocephala, 5-6 parts of Leonurus japonicus, 2-3 parts of Polygala tenuifolia, and 2-3 parts of Panax ginseng leaves.

4. Use of the Chinese herbal medicine formula according to any one of claims 1 to 3 in the preparation of a feed additive for laying fowl.

5. A feed additive for laying fowl, characterized in that: The invention comprises the Chinese herbal medicine formula according to any one of claims 1 to 3.

6. A laying fowl feed, characterized in that: The feed comprises: a basic diet and the Chinese herbal medicine formula according to any one of claims 1 to 3.

7. The laying fowl feed according to claim 6, characterized in that The added amount of the Chinese herbal medicine formula is 0.5-1% of the weight of the basic diet.

8. The layer poultry feed according to claim 6 or 7, characterized in that The egg-laying poultry includes chickens, ducks, geese and quails.

9. A method for improving the production performance of laying fowl, characterized in that: include: The laying fowl is fed the Chinese herbal medicine formula according to any one of claims 1 to 3 twice a day.

10. The method according to claim 9, characterized in that The Chinese herbal formula is mixed with the basic diet and fed to the pigs. The addition amount of the Chinese herbal formula is 0.5-1% of the weight of the basic diet, preferably 0.7-1%.

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