Liquid path system and method, sequencing system and storage medium

Abstract

The invention discloses a liquid path system and method, a sequencing system and a storage medium. The liquid path system comprises a fluid device, the fluid device comprises a manifold assembly, a fluid flowing unit and a power assembly, the fluid flowing unit comprises at least one fluid channel, the fluid flowing unit is communicated with the manifold assembly through the fluid channel, the power assembly is connected with the fluid flowing unit, and the power assembly is used for providing power. Liquid is caused to enter the fluid device and flow back and forth between the manifold assembly and the fluid channel. According to the liquid path system, the power assembly drives the liquid to flow back and forth between the manifold assembly and the fluid channel, residues on the manifold assembly can be fully mixed and / or replaced, crystals formed by the residues on the manifold assembly can be fully redissolved, and therefore the manifold assembly can be conveniently cleaned, and the sequencing effect is improved.

Description

Technical Field

[0001] The present invention relates to the field of gene sequencing, and in particular to a liquid circuit system, method, sequencing system and storage medium. Background Art

[0002] Gene sequencing technology refers to the technical means of obtaining DNA or RNA base sequences through detection. The currently dominant sequencing technology is high-throughput sequencing technology. In sequencing platforms based on sequencing by synthesis to achieve high-throughput sequencing, such as ILLUMINA's Miseq, Nextseq, and Novasek sequencing platforms, the general process of gene sequencing includes fixing the nucleic acid sample to be tested on a flow cell, such as by hybridization; then using PCR amplification to form nucleic acid molecule clusters from the nucleic acid sample to be tested; then adding sequencing reagents, such as bases with fluorescent groups, polymerases, and primers, to the flow cell, and through the principle of base complementary pairing, the bases with fluorescent groups are combined with bases on the nucleic acid sample to be tested; finally, using an optical imaging system to excite the fluorescent groups to generate fluorescence and collect the fluorescence to form an image, and then performing base recognition based on the image to achieve base sequence determination of the nucleic acid sample to be tested.

[0003] After sequencing is completed, when the flow cell is removed or replaced or other operations that affect the flow cell are performed, liquids such as sequencing reagents after the reaction may flow out of the flow cell and remain on the sequencing platform. If these liquids and / or the crystals formed are not removed in time, the progress and effect of sequencing will be affected. Summary of the Invention

[0004] The present invention provides a liquid circuit system, method, sequencing system and storage medium.

[0005] An embodiment of the present application provides a fluid circuit system, which includes a fluid device, which includes a manifold assembly, a fluid flow unit and a power assembly. The fluid flow unit includes at least one fluid channel, the fluid flow unit is connected to the manifold assembly through the fluid channel, and the power assembly is connected to the fluid flow unit. The power assembly is used to provide power to allow liquid to enter the fluid device and flow back and forth between the manifold assembly and the fluid channel.

[0006] In the liquid path system of the embodiment of the present application, the power component drives the liquid to flow back and forth between the manifold assembly and the fluid channel, which can fully mix and / or replace the residue on the manifold assembly, and can also fully dissolve the crystals formed by the residue on the manifold assembly, thereby facilitating the cleaning of the manifold assembly and improving the sequencing effect.

[0007] In some embodiments, the manifold assembly includes a first manifold member and a second manifold member, at least one liquid inlet is provided on the first manifold member, and at least one liquid outlet is provided on the second manifold member, the liquid inlet and the liquid outlet are provided in a one-to-one correspondence, the fluid flow unit is located between the first manifold member and the second manifold member, each fluid channel includes a channel inlet and a channel outlet, the first manifold member is connected to the fluid channel through the liquid inlet and the channel inlet, and the second manifold member is connected to the fluid channel through the liquid outlet and the channel outlet.

[0008] In this way, liquid can enter the fluid channel from the liquid inlet and flow channel inlet of the first manifold component, and can also flow out of the fluid channel from the flow channel outlet and the liquid outlet of the second manifold component, so that the liquid can flow back and forth between the manifold assembly and the fluid channel.

[0009] In some embodiments, the flow channel inlet and the flow channel outlet each have a first central axis, the liquid inlet and the liquid outlet each have a second central axis, the first central axis of the flow channel inlet is set offset from the second central axis of the liquid inlet, and the first central axis of the flow channel outlet is set offset from the second central axis of the liquid outlet.

[0010] In this way, the flow channel inlet and the liquid inlet are eccentric, and the flow channel outlet and the liquid outlet are eccentric, so that turbulence is formed when the liquid flows from the liquid inlet to the flow channel inlet and from the flow channel outlet to the liquid outlet, which can fully mix and / or replace the residues remaining at the liquid inlet and the liquid outlet, and can also fully dissolve the crystals formed by the residues remaining at the liquid inlet and the liquid outlet, thereby facilitating the cleaning of the manifold assembly.

[0011] In some embodiments, a first sealing gasket is provided between the first manifold and the fluid flow unit, and a second sealing gasket is provided between the second manifold and the fluid flow unit. At least one first liquid hole is provided on the first sealing gasket, and at least one second liquid hole is provided on the second sealing gasket. The first liquid hole and the liquid inlet are provided in a one-to-one correspondence, and the second liquid hole and the liquid outlet are provided in a one-to-one correspondence. The liquid inlet is connected to the flow channel inlet through the first liquid hole, and the liquid outlet is connected to the flow channel outlet through the second liquid hole.

[0012] Thus, disposing a first sealing gasket between the first manifold and the fluid flow unit can improve the sealing performance between the first manifold and the fluid flow unit, prevent liquid from flowing out of the gap between the first manifold and the fluid flow unit, and reduce liquid waste. Disposing a second sealing gasket between the second manifold and the fluid flow unit can improve the sealing performance between the second manifold and the fluid flow unit, prevent liquid from flowing out of the gap between the second manifold and the fluid flow unit, and reduce liquid waste.

[0013] In some embodiments, the first liquid through hole and the second liquid through hole each have a third central axis, and the third central axis is coaxially arranged with the second central axis.

[0014] In this way, the third central axis is coaxially arranged with the second central axis, so that the first sealing gasket is conveniently connected to the first manifold member, and the second sealing gasket is conveniently connected to the second manifold member, thereby improving the convenience of operation.

[0015] In some embodiments, the first sealing gasket includes a first gasket body and a first flange, the first liquid hole is provided on the first gasket body, and the first flange is provided around the first liquid hole; the second sealing gasket includes a second gasket body and a second flange, the second liquid hole is provided on the second gasket body, and the second flange is provided around the second liquid hole.

[0016] In this way, the first flange can improve the sealing performance between the first manifold member and the first sealing gasket, preventing liquid from flowing out of the gap between the first gasket body and the first manifold member, thereby reducing liquid waste. The second flange can improve the sealing performance between the second manifold member and the second sealing gasket, preventing liquid from flowing out of the gap between the second gasket body and the second manifold member, thereby reducing liquid waste.

[0017] In certain embodiments, the fluid system includes a stage device, the manifold assembly and the fluid flow unit are disposed on the stage device, and the fluid flow unit is detachably connected to the stage device.

[0018] In this way, the manifold assembly and the fluid flow unit are arranged on the stage device, which can improve the stability of the fluid flow unit and thus enhance the sequencing accuracy.

[0019] In some embodiments, the stage device includes a stage having a carrying surface, and the fluid flow unit is located on the carrying surface.

[0020] In this way, the fluid flow unit is located on the carrying surface and can be communicated with the manifold assembly on the stage device.

[0021] In some embodiments, a positioning hole is provided on the bearing surface, and a positioning pin is provided on the fluid flow unit. The positioning hole and the positioning pin are plugged into each other to enable the fluid flow unit to be detachably mounted on the bearing surface.

[0022] In this way, the fluid flow unit and the carrier platform can be accurately positioned by plugging and matching the positioning holes and the positioning pins, thereby improving the assembly accuracy of the fluid flow unit and the carrier platform, and making the fluid flow unit easy to disassemble and assemble, and convenient to operate.

[0023] In some embodiments, a groove is formed inwardly on the bearing surface.

[0024] In this way, negative pressure can be formed at the opening of the channel on the carrying surface, thereby fixing the fluid flow unit on the carrying surface.

[0025] In certain embodiments, the channel includes at least one first channel and at least one second channel, and the first channel and the second channel are connected to each other.

[0026] In this way, by providing at least one first channel and at least one second channel in communication, a plurality of openings can be formed on the table surface for adsorbing the fluid flow unit, thereby fixing the fluid flow unit on the table surface.

[0027] In some embodiments, the first channel and the second channel are arranged to cross each other.

[0028] In this way, by arranging the first groove and the second groove in a cross manner, the coverage of the first groove and the second groove on the carrying surface can be improved, which can be applicable to fluid flow units of different sizes and improve the adsorption effect of the carrier device.

[0029] In some embodiments, there are multiple first grooves, and the multiple first grooves are spaced apart and arranged in parallel. There are multiple second grooves, and the multiple second grooves are spaced apart and arranged in parallel. The first grooves and the second grooves are perpendicular to each other.

[0030] In this way, multiple first grooves and multiple second grooves are arranged vertically across each other, so that the first grooves and the second grooves can form openings in a grid layout on the supporting surface. When the fluid flow unit is placed on the supporting surface, the fluid flow unit can be adsorbed and fixed by generating negative pressure at the openings of the first grooves and the second grooves.

[0031] In certain embodiments, the fluid circuit system includes a vacuum device, an output port communicating with the fluid channel is provided on the fluid flow unit, and the vacuum device is communicated with the fluid channel through the output port.

[0032] In this way, the vacuum device can generate negative pressure on the fluid channel through the output port, and further generate negative pressure on the manifold assembly, thereby sucking away the residual liquid attached to the manifold assembly.

[0033] In certain embodiments, there are multiple fluid channels, and the output ports on the multiple fluid channels are connected to the vacuum device.

[0034] In this way, the vacuum device can generate negative pressure on the multiple fluid channels through the output port to absorb residual liquid adhering to the surfaces of the multiple liquid inlets and liquid outlets of the manifold assembly that contact the first and second sealing gaskets.

[0035] In some embodiments, the vacuum device includes a vacuum pump and a first switching valve, the first switching valve having a first port and two second ports, the first switching valve being connected to the vacuum pump through the first port, the first switching valve being connected to the channel through one of the second ports, and the first switching valve being connected to the output port through the other second port.

[0036] In this way, the channel can be connected to the vacuum pump through the first switching valve to adsorb the fluid flow unit on the carrier; the output port can also be connected to the vacuum pump to absorb the residual liquid attached to the manifold assembly.

[0037] In some embodiments, the vacuum device also includes a second switching valve, which is connected to the first switching valve and is configured to switch between a first working state and a second working state; when the second switching valve is in the first working state, the second switching valve is connected to the vacuum pump so that the vacuum pump can be connected to the channel or output port through the second switching valve and the first switching valve in sequence; when the second switching valve is in the second working state, the connection between the second switching valve and the vacuum pump is disconnected, and the second switching valve is connected to the external atmosphere so that the channel or output port can be connected to the external atmosphere through the first switching valve and the second switching valve in sequence.

[0038] In this way, the second switching valve can be used to connect the channel or output port to the vacuum pump to adsorb the fluid flow unit onto the carrier or absorb the residual liquid attached to the manifold assembly; the second switching valve can also be used to connect the channel or output port to the external atmosphere to facilitate the removal and placement of the fluid flow unit.

[0039] In some embodiments, the second switching valve includes a third port, a fourth port, and a fifth port, the vacuum device includes a first pipeline, a second pipeline, and a third pipeline, one end of the first pipeline is connected to the third port of the second switching valve, and the other end is connected to the first port of the first switching valve, the second pipeline and the third pipeline are respectively connected to the fourth port and the fifth port of the second switching valve, and the vacuum pump is arranged on the second pipeline.

[0040] In this way, by selecting the fourth port and the fifth port, the second switching valve can be switched between the first working state and the second working state.

[0041] In certain embodiments, a gas-water separation component is provided on the first pipeline, and the gas-water separation component is used to separate the gas and liquid entering the first pipeline.

[0042] In this way, the gas and liquid entering the first pipeline can be separated by the gas-water separation component, preventing the liquid from entering the vacuum pump, thereby affecting the vacuum adsorption effect and service life of the vacuum pump.

[0043] In certain embodiments, a muffler is provided on the second pipeline.

[0044] In this way, the muffler can reduce the exhaust noise of the vacuum pump, protect the quality of the working environment of the liquid system, and improve work efficiency.

[0045] In certain embodiments, an air filter is provided on the third line.

[0046] In this way, the air filter can filter impurities in the gas entering the third pipeline, preventing the impurities from contaminating the liquid system through the third pipeline, thereby affecting the adsorption performance of the vacuum pump and the sequencing accuracy.

[0047] In certain embodiments, the power assembly includes a syringe pump configured to provide power to allow liquid to enter the fluidic device and flow back and forth between the manifold assembly and the fluidic channel.

[0048] In this way, the syringe pump can provide power to drive the liquid back and forth between the manifold assembly and the fluid channels.

[0049] An embodiment of the present application provides a method for controlling the flow of liquid in a fluid circuit system, wherein the fluid circuit system includes:

[0050] A fluid device, the fluid device includes a manifold assembly, a fluid flow unit and a power assembly, the fluid flow unit includes at least one fluid channel, and the power assembly is connected to the fluid flow unit;

[0051] The method comprises:

[0052] a), connecting the manifold assembly with the fluid channel;

[0053] b) driving the liquid into the fluid channel through the manifold assembly using a power assembly;

[0054] c) driving the liquid from the fluid channel back to the manifold assembly using a power assembly;

[0055] Repeat steps b) and c).

[0056] In this way, through the above steps, the liquid can flow back and forth between the manifold assembly and the fluid channel, which can fully mix and / or replace the residues on the manifold assembly, and can also fully dissolve the crystals formed by the residues remaining on the manifold assembly, thereby facilitating the cleaning of the manifold assembly and improving the sequencing effect.

[0057] In certain embodiments, the method comprises:

[0058] d) moving the fluid flow unit to disconnect the fluid channel from the manifold assembly and connect the fluid channel to the vacuum device;

[0059] e) using a vacuum device to create a negative pressure between the manifold assembly and the fluid channel to remove residual liquid attached to the manifold assembly through the fluid channel;

[0060] d) and e) are performed after a), b) and c) are completed.

[0061] In this way, the residual liquid adhering to the manifold assembly can be sucked away through the above steps. d) Performing step d) after steps a), b), and c) is helpful in preventing residue and / or crystals formed from the residue from clogging the pipeline and affecting the adsorption effect and service life of the vacuum device.

[0062] An embodiment of the present application provides a sequencing system, which includes a liquid circuit system.

[0063] In the sequencing system of the embodiment of the present application, the liquid path system drives the liquid to flow back and forth between the manifold assembly and the fluid channel through the power assembly, which can fully mix or replace the residue on the manifold assembly, and can also fully dissolve the crystals formed by the residue remaining on the manifold assembly, thereby cleaning the manifold assembly and improving the sequencing effect.

[0064] An embodiment of the present application provides a non-volatile computer-readable storage medium storing a computer program. When the computer program is executed by one or more processors, the method of any of the above embodiments is implemented.

[0065] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0066] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments with reference to the following drawings, in which:

[0067] Figure 1 Schematic diagram of the structure of the liquid circuit system according to an embodiment of the present invention;

[0068] Figure 2 Schematic diagram of the structure of the fluid flow unit and the stage device according to an embodiment of the present invention;

[0069] Figure 3 is an exploded view of a fluid flow unit and a stage device according to an embodiment of the present invention;

[0070] Figure 4 Schematic diagram of the structure of the fluid flow unit and the stage device according to an embodiment of the present invention;

[0071] Figure 5 yes Figure 4 Schematic cross-sectional view along the AA direction;

[0072] Figure 6 is a schematic structural diagram of a first manifold member according to an embodiment of the present invention;

[0073] Figure 7 is a schematic structural diagram of a second manifold member according to an embodiment of the present invention;

[0074] Figure 8 yes Figure 5 An enlarged schematic diagram of part I;

[0075] Figure 9 yes Figure 5 An enlarged schematic diagram of part II;

[0076] Figure 10 is a schematic structural diagram of a fluid flow unit according to an embodiment of the present invention;

[0077] Figure 11 is a schematic structural diagram of a fluid flow unit according to an embodiment of the present invention;

[0078] Figure 12 1 is a schematic structural diagram of a stage device according to an embodiment of the present invention;

[0079] Figure 13 Schematic diagram of the structure of the fluid flow unit and the stage device according to an embodiment of the present invention;

[0080] Figure 14 yes Figure 13 Schematic cross-sectional view along direction BB;

[0081] Figure 15 is a schematic flow chart of a method according to an embodiment of the present invention;

[0082] Figure 16 It is a flow chart of a method according to an embodiment of the present invention.

[0083] Explanation of reference numerals: 1000, fluid circuit system; 100, fluid device; 10, manifold assembly; 11, first manifold member; 12, liquid inlet; 13, second manifold member; 14, liquid outlet; 15, second central axis; 20, fluid flow unit; 21, fluid channel; 22, flow channel inlet; 23, flow channel outlet; 24, first central axis; 25, positioning pin; 26, output port; 30, power assembly; 31, injection pump; 32, syringe; 33, four-port valve head; 34, waste liquid tank; 40, first sealing gasket; 41, first liquid hole; 42, first gasket body; 43, first flange; 44, second sealing gasket; 45, second liquid hole; 46, second gasket body; 47, second flange; 48, The third central axis; 200, the carrier device; 210, the carrier platform; 201, the carrier surface; 202, the positioning hole; 203, the groove; 204, the first groove; 205, the second groove; 206, the adsorption hole; 207, the sealing ring; 300, the vacuum device; 301, the air path connector; 310, the vacuum pump; 320, the first switching valve; 321, the first port; 322, the second port; 330, the second switching valve; 331, the third port; 332, the fourth port; 333, the fifth port; 340, the first pipeline; 341, the air-water separation component; 342, the pressure detection component; 343, the vacuum air pressure valve; 350, the second pipeline; 351, the muffler; 360, the third pipeline; 361, the air filter. DETAILED DESCRIPTION

[0084] The embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and are not to be construed as limiting the present invention.

[0085] The disclosure below provides many different embodiments or examples for realizing different structures of the present invention. In order to simplify the disclosure of the present invention, the components and settings of specific examples are described below. Of course, they are merely examples and are not intended to limit the present invention. In addition, the present invention may repeat reference numbers and / or reference letters in different examples. Such repetition is for the purpose of simplicity and clarity and does not in itself indicate the relationship between the various embodiments and / or settings discussed. In addition, the present invention provides examples of various specific processes and materials, but those skilled in the art will recognize the application of other processes and / or the use of other materials.

[0086] See also Figure 1-Figure 5An embodiment of the present application provides a fluid circuit system 1000, which includes a fluid device 100. The fluid device 100 includes a manifold assembly 10, a fluid flow unit 20, and a power assembly 30. The fluid flow unit 20 includes at least one fluid channel 21. The fluid flow unit 20 is connected to the manifold assembly 10 through the fluid channel 21. The power assembly 30 is connected to the fluid flow unit 20. The power assembly 30 is used to provide power to enable liquid to enter the fluid device 100 and flow back and forth between the manifold assembly 10 and the fluid channel 21.

[0087] In the liquid path system 1000 of the embodiment of the present application, the power component 30 drives the liquid to flow back and forth between the manifold component 10 and the fluid channel 21, which can fully mix and / or replace the residue on the manifold component 10, and can also fully dissolve the crystals formed by the residue on the manifold component 10, thereby facilitating the cleaning of the manifold component 10 and improving the sequencing effect.

[0088] Specifically, the manifold assembly 10 may be composed of a plurality of manifold members. In some embodiments, the fluid device 100 is provided with a support member and an elastic member, which are respectively connected to the manifold assembly 10 and the support member to drive the manifold assembly 10 to abut against the fluid flow unit 20.

[0089] The fluid flow unit 20 may include a flow cell or chip, which communicates with the manifold assembly 10 to provide a path for liquid flow. The fluid flow unit 20 may also include other components capable of communicating with the manifold assembly 10 to provide a path for liquid flow, such as a pipe having a fluid channel 21 to facilitate cleaning of the manifold assembly 10. The cross-sectional shape of the fluid channel 21 may be circular or square, and multiple fluid channels 21 may be arranged at intervals along the width of the flow cell. The number of fluid channels 21 may be multiple, such as two, three, four, or five.

[0090] The power assembly 30 includes but is not limited to equipment capable of providing power and pipelines for transmitting power. The power assembly 30 can be connected to the manifold assembly 10 through a pipeline to drive the liquid to flow back and forth between the manifold assembly 10 and the fluid channel 21.

[0091] The liquid may be, for example, a cleaning fluid, including but not limited to one or more of water, an organic solvent, and an inorganic solvent. The residue may be, for example, a sequencing reagent having a certain concentration. When sequencing is completed and the sequencing chip is removed, the residue may remain on the manifold assembly 10, and the residue is susceptible to crystallization when exposed to an open environment. The liquid flowing back and forth between the manifold assembly 10 and the fluid channel 21 can dilute, replace, and / or dissolve any crystals formed by the residue.

[0092] See also Figure 5-Figure 7In some embodiments, the manifold assembly 10 includes a first manifold member 11 and a second manifold member 13. At least one liquid inlet 12 is provided on the first manifold member 11, and at least one liquid outlet 14 is provided on the second manifold member 13. The liquid inlet 12 and the liquid outlet 14 are provided in a one-to-one correspondence. The fluid flow unit 20 is located between the first manifold member 11 and the second manifold member 13. Each fluid channel 21 includes a channel inlet 22 and a channel outlet 23. The first manifold member 11 is connected to the fluid channel 21 through the liquid inlet 12 and the channel inlet 22, and the second manifold member 13 is connected to the fluid channel 21 through the liquid outlet 14 and the channel outlet 23.

[0093] In this way, the liquid can enter the fluid channel 21 from the liquid inlet 12 and the flow channel inlet 22 of the first manifold component 11, and can also flow out of the fluid channel 21 from the flow channel outlet 23 and the liquid outlet 14 of the second manifold component 13, thereby realizing the back and forth flow of liquid between the manifold assembly 10 and the fluid channel 21.

[0094] Specifically, the first manifold member 11 and the second manifold member 13 can be made of metal, plastic, or other materials. The first manifold member 11 and the second manifold member 13 can be integrally injection-molded from two rectangular blocks. The first manifold member 11 can have multiple liquid inlets 12, such as two, three, four, or five, and the multiple liquid inlets 12 are spaced apart along the length of the first manifold member 11. The shape of the liquid inlets 12 can be circular or square. The second manifold member 13 can have multiple liquid outlets 14, such as two, three, four, or five, and the multiple liquid outlets 14 are spaced apart along the length of the second manifold member 13. The shape of the liquid outlets 14 can be circular or square.

[0095] The channel inlet 22 and the channel outlet 23 are respectively located at the two ends of the fluid channel 21. The size of the channel inlet 22 can be larger than the size of the liquid inlet 12, and the size of the channel outlet 23 can also be larger than the size of the liquid outlet 14. That is to say, when the channel inlet 22, the channel outlet 23, the liquid inlet 12 and the liquid outlet 14 are circular, the radius of the channel inlet 22 can be larger than the radius of the liquid inlet 12, and the radius of the channel outlet 23 can also be larger than the radius of the liquid outlet 14.

[0096] See also Figure 5 、 Figure 8 and Figure 9 In some embodiments, the flow channel inlet 22 and the flow channel outlet 23 each have a first central axis 24, the liquid inlet 12 and the liquid outlet 14 each have a second central axis 15, the first central axis 24 of the flow channel inlet 22 is set to deviate from the second central axis 15 of the liquid inlet 12, and the first central axis 24 of the flow channel outlet 23 is set to deviate from the second central axis 15 of the liquid outlet 14.

[0097] In this way, the flow channel inlet 22 is eccentric to the liquid inlet 12, and the flow channel outlet 23 is eccentric to the liquid outlet 14, so that turbulence is formed when the liquid flows from the liquid inlet 12 to the flow channel inlet 22 and from the flow channel outlet 23 to the liquid outlet 14, which can fully mix and / or replace the residues remaining at the liquid inlet 12 and the liquid outlet 14, and can also fully dissolve the crystals formed by the residues remaining at the liquid inlet 12 and the liquid outlet 14, thereby facilitating the cleaning of the manifold assembly 10.

[0098] Specifically, when the flow channel inlet 22 and the flow channel outlet 23 are circular in shape, the first central axis 24 can be located at the center of the flow channel inlet 22 and the flow channel outlet 23; when the liquid inlet 12 and the liquid outlet 14 are circular in shape, the second central axis 15 can be located at the center of the liquid inlet 12 and the liquid outlet 14. The first central axis 24 can be arranged parallel to the second central axis 15.

[0099] See also Figure 5 and Figure 10 In some embodiments, a first sealing gasket 40 is provided between the first manifold 11 and the fluid flow unit 20, and a second sealing gasket 44 is provided between the second manifold 13 and the fluid flow unit 20. At least one first liquid hole 41 is provided on the first sealing gasket 40, and at least one second liquid hole 45 is provided on the second sealing gasket 44. The first liquid holes 41 are provided in a one-to-one correspondence with the liquid inlet 12, and the second liquid holes 45 are provided in a one-to-one correspondence with the liquid outlet 14. The liquid inlet 12 is connected to the channel inlet 22 through the first liquid hole 41, and the liquid outlet 14 is connected to the channel outlet 23 through the second liquid hole 45.

[0100] Thus, the first sealing gasket 40 is provided between the first manifold 11 and the fluid flow unit 20 to improve the sealing performance between the first manifold 11 and the fluid flow unit 20, prevent liquid from flowing out of the gap between the first manifold 11 and the fluid flow unit 20, and reduce liquid waste. The second sealing gasket 44 is provided between the second manifold 13 and the fluid flow unit 20 to improve the sealing performance between the second manifold 13 and the fluid flow unit 20, prevent liquid from flowing out of the gap between the second manifold 13 and the fluid flow unit 20, and reduce liquid waste.

[0101] Specifically, the first sealing gasket 40 and the second sealing gasket 44 can be made of a rubber material and can be in the shape of a rectangular parallelepiped. The first liquid hole 41 and the second liquid hole 45 can be through holes. The first liquid hole 41 penetrates the first sealing gasket 40 along the thickness direction of the first sealing gasket 40, and the second liquid hole 45 penetrates the second sealing gasket 44 along the thickness direction of the second sealing gasket 44.

[0102] The first liquid passage hole 41 and the second liquid passage hole 45 can be circular in shape. The radius of the first liquid passage hole 41 can be larger than the radius of the liquid inlet 12 or the radius of the flow channel inlet 22. The radius of the second liquid passage hole 45 can be larger than the radius of the liquid outlet 14 or the radius of the flow channel outlet 23. The number of first liquid passage holes 41 and second liquid passage holes 45 can be multiple, for example, two, three, four, etc. The multiple first liquid passage holes 41 can be arranged at intervals along the length of the first sealing gasket 40, and the multiple second liquid passage holes 45 can be arranged at intervals along the length of the second sealing gasket 44.

[0103] See also Figure 5 、 Figure 8 and Figure 9 In some embodiments, the first liquid through hole 41 and the second liquid through hole 45 each have a third central axis 48 , and the third central axis 48 is coaxially arranged with the second central axis 15 .

[0104] In this way, the third central axis 48 is coaxially arranged with the second central axis 15, so that the first sealing gasket 40 is conveniently connected to the first manifold 11, and the second sealing gasket 44 is conveniently connected to the second manifold 13, thereby improving the convenience of operation.

[0105] Specifically, the third central axis 48 can be coaxial with the second central axis 15, with the second central axis 15 offset from the first central axis 24. Alternatively, the third central axis 48 can be coaxial with the first central axis 24, with the second central axis 15 offset from the first central axis 24. The third central axis 48 can be parallel to the second central axis 15.

[0106] See also Figure 10 and Figure 11 In some embodiments, the first sealing gasket 40 includes a first gasket body 42 and a first flange 43, the first liquid hole 41 is provided on the first gasket body 42, and the first flange 43 is provided around the first liquid hole 41; the second sealing gasket 44 includes a second gasket body 46 and a second flange 47, the second liquid hole 45 is provided on the second gasket body 46, and the second flange 47 is provided around the second liquid hole 45.

[0107] In this way, the first flange 43 can improve the sealing performance between the first manifold member 11 and the first sealing gasket 40, preventing liquid from flowing out of the gap between the first gasket body 42 and the first manifold member 11, thereby reducing liquid waste. The second flange 47 can improve the sealing performance between the second manifold member 13 and the second sealing gasket 44, preventing liquid from flowing out of the gap between the second gasket body 46 and the second manifold member 13, thereby reducing liquid waste.

[0108] Specifically, the first pad body 42 and the second pad body 46 may be in the shape of a rectangular parallelepiped. The first flange 43 may extend along the thickness direction of the first pad body 42 and be disposed proximate to the first manifold 11. The first flange 43 may be integrally formed with the first pad body 42 or machined from the first pad body 42. The second flange 47 may extend along the thickness direction of the second pad body 46 and be disposed proximate to the second manifold 13. The second flange 47 may be integrally formed with the second pad body 46 or machined from the second pad body 46. The thickness of the first pad body 42 may be greater than the distance between the fluid flow unit 20 and the first manifold 11, and the thickness of the second pad body 46 may be greater than the distance between the fluid flow unit 20 and the second manifold 13.

[0109] See also Figure 4 and Figure 12 In some embodiments, the fluid system 1000 includes a stage device 200, the manifold assembly 10 and the fluid flow unit 20 are disposed on the stage device 200, and the fluid flow unit 20 and the stage device 200 are detachably connected.

[0110] In this way, the manifold assembly 10 and the fluid flow unit 20 are arranged on the stage device 200, which can improve the stability of the fluid flow unit 20 and thus enhance the sequencing accuracy.

[0111] Specifically, the carrier device 200 can carry the manifold assembly 10 and the fluid flow unit 20, the manifold assembly 10 can be snapped onto the carrier device 200, the manifold assembly 10 can move relative to the carrier device along the height direction of the carrier device, and the fluid flow unit 20 can be fixed on the carrier device 200 by using detachable connection methods such as vacuum adsorption, magnetic attraction, bonding, and snap connection.

[0112] See also Figure 12 and Figure 13 In some embodiments, the stage device 200 includes a carrying platform 210 , the carrying platform 210 has a carrying surface 201 , and the fluid flow unit 20 is located on the carrying surface 201 .

[0113] In this way, the fluid flow unit 20 is located on the carrying surface 201 and can be communicated with the manifold assembly 10 on the stage device 200 .

[0114] Specifically, the shape of the support platform 210 can be a cuboid, the shape of the support surface 201 can be a rectangle, and the size of the support surface 201 can be smaller than the size of the fluid flow unit 20. The fluid flow unit 20 can be fixed to the support surface 201 by a detachable connection method such as vacuum adsorption, magnetic attraction, bonding, or snap connection.

[0115] See also Figure 10 、 Figure 12 and Figure 14In some embodiments, a positioning hole 202 is provided on the bearing surface 201 , and a positioning pin 25 is provided on the fluid flow unit 20 . The positioning hole 202 is plugged into and matched with the positioning pin 25 , so that the fluid flow unit 20 can be detachably mounted on the bearing surface 201 .

[0116] In this way, by plugging and matching the positioning hole 202 with the positioning pin 25, the fluid flow unit 20 and the carrier 210 can be accurately positioned, thereby improving the assembly accuracy of the fluid flow unit 20 and the carrier 210, and making the fluid flow unit 20 easy to disassemble and assemble, and convenient to operate.

[0117] Specifically, the positioning hole 202 can penetrate the supporting platform 210 along its height, and the positioning pin 25 can be disposed near the supporting surface 201 along its thickness. The positioning hole 202 and the positioning pin 25 can have circular cross-sections, and the radius of the positioning hole 202 can be greater than the radius of the positioning pin 25. There can be multiple positioning holes 202 and positioning pins 25, for example, two, three, or four, with the multiple positioning holes 202 and positioning pins 25 corresponding to each other.

[0118] See also Figure 12 In some embodiments, a groove 203 is formed inwardly on the bearing surface 201 .

[0119] In this way, negative pressure can be formed at the opening of the groove 203 on the carrying surface 201 to fix the fluid flow unit 20 on the carrying surface 201 .

[0120] Specifically, the grooves 203 can be arranged in a regular shape such as a square or a circle on the bearing surface 201, or can be arranged in an irregular shape. The shape and depth of the grooves 203 can be set according to actual needs. The positioning holes 202 can be set in the grooves 203.

[0121] See also Figure 12 In some embodiments, the channel 203 includes at least one first channel 204 and at least one second channel 205 , and the first channel 204 and the second channel 205 are connected to each other.

[0122] In this way, by providing at least one first channel 204 and at least one second channel 205 in communication, a plurality of openings can be formed on the table surface for adsorbing the fluid flow unit 20 and fixing the fluid flow unit 20 on the table surface.

[0123] Specifically, the number of first grooves 204 and second grooves 205 can be one, two, three, four, etc. When there are multiple first grooves 204 and second grooves 205, the adsorption range of the support surface 201 can be further increased, thereby improving the stability of the stage assembly 200 in adsorbing the fluid flow unit 20. The cross-sectional shape of the first groove 204 and the second groove 205 can be rectangular or arc-shaped. The positioning hole 202 can be provided in the first groove 204, the second groove 205, or the connection between the first groove 204 and the second groove 205.

[0124] See also Figure 12 In some embodiments, the first channel 204 and the second channel 205 are arranged to cross each other.

[0125] In this way, by arranging the first groove 204 and the second groove 205 in a cross manner, the coverage of the first groove 204 and the second groove 205 on the carrying surface 201 can be improved, which can be applicable to fluid flow units 20 of different sizes and improve the adsorption effect of the carrier device 200.

[0126] Specifically, the angle between the first groove 204 and the second groove 205 can be an acute angle, a right angle, or an obtuse angle. The positioning hole 202 can be provided at the intersection of the first groove 204 and the second groove 205 .

[0127] See also Figure 12 In some embodiments, there are multiple first grooves 204, and the multiple first grooves 204 are spaced apart and arranged in parallel. There are multiple second grooves 205, and the multiple second grooves 205 are spaced apart and arranged in parallel. The first grooves 204 and the second grooves 205 are perpendicular to each other.

[0128] In this way, multiple first grooves 204 and multiple second grooves 205 are arranged vertically across each other, so that the first grooves 204 and the second grooves 205 can form openings in a grid layout on the supporting surface 201. When the fluid flow unit 20 is placed on the supporting surface 201, the fluid flow unit 20 can be adsorbed and fixed by generating negative pressure at the openings of the first grooves 204 and the second grooves 205.

[0129] Specifically, the number of first grooves 204 can be three, four, or five, and the number of second grooves 205 can be five, six, or seven, etc. The first grooves 204 can be parallel to the length direction of the bearing surface 201, and the second grooves 205 can be parallel to the width direction of the bearing surface 201; alternatively, the first grooves 204 can be parallel to the width direction of the bearing surface 201, and the second grooves 205 can be parallel to the length direction of the bearing surface 201.

[0130] See also Figure 1 and Figure 14 In some embodiments, the fluid system 1000 includes a vacuum device 300 , and an output port 26 connected to the fluid channel 21 is provided on the fluid flow unit 20 , and the vacuum device 300 is connected to the fluid channel 21 through the output port 26 .

[0131] In this way, the vacuum device 300 can generate negative pressure on the fluid channel 21 through the output port 26 , and further generate negative pressure on the manifold assembly 10 , thereby sucking away the residual liquid attached to the manifold assembly 10 .

[0132] Specifically, the vacuum device 300 includes but is not limited to a vacuum pump 310, a vacuum suction cup, etc. The vacuum device 300 can be connected to the output port 26 of the fluid channel 21 through an air connector 301. The shape of the output port 26 can be regular shapes such as circular, polygonal, or irregular. In the embodiment of the present application, in order to facilitate the formation, manufacture and / or connection of the output port 26 with a common air connector 301, the output port 26 is circular. Figure 12 In some embodiments, an adsorption hole 206 is provided on the groove 203, and the vacuum device 300 can be connected to the adsorption hole 206 through the air path connector 301 to generate negative pressure to extract the air in the groove 203, so as to adsorb and fix the fluid flow unit 20 on the supporting surface 201.

[0133] Residual liquid, for example, can be liquid mixed with residues remaining around the liquid inlet 12 and / or liquid outlet 14 of the manifold assembly 10. Residual liquid can be left after the fluid flow unit 20 is removed when the diameter of the first liquid passage 41 is larger than the diameter of the liquid inlet 12, and the diameter of the second liquid passage 45 is larger than the diameter of the liquid outlet 14. The residual liquid is removed by vacuum device 300 to further clean the manifold assembly 10 and improve sequencing performance.

[0134] See also Figure 1 and Figure 14 In some embodiments, there are multiple fluid channels 21 , and the output ports 26 on the multiple fluid channels 21 are connected to the vacuum device 300 .

[0135] In this way, the vacuum device 300 can generate negative pressure on the multiple fluid channels 21 through the output port 26 to absorb residual liquid attached to the surfaces of the multiple liquid inlets 12 and liquid outlets 14 of the manifold assembly 10 that contact the first and second sealing gaskets 40 and 44.

[0136] Specifically, the number of fluid channels 21 can be two, three, four, etc., and the number of output ports 26 can be multiple, for example, two, three, four, etc. When the number of fluid channels 21 is four, the number of output ports 26 can be two or four. In other words, one fluid channel 21 can correspond to one output port 26, or two adjacent fluid channels 21 can be interconnected, and the two interconnected fluid channels 21 correspond to one output port 26.

[0137] See also Figure 1 、 Figure 11 and Figure 12 In some embodiments, the vacuum device 300 includes a vacuum pump 310 and a first switching valve 320, the first switching valve 320 having a first port 321 and two second ports 322, the first switching valve 320 is connected to the vacuum pump 310 through the first port 321, the first switching valve 320 is connected to the channel 203 through one of the second ports 322, and the first switching valve 320 is connected to the output port 26 through the other second port 322.

[0138] In this way, the channel 203 can be connected to the vacuum pump 310 through the first switching valve 320 to adsorb the fluid flow unit 20 on the carrier 210; the output port 26 can also be connected to the vacuum pump 310 to absorb the residual liquid attached to the manifold assembly 10.

[0139] Specifically, the vacuum pump 310 refers to a device or apparatus that uses mechanical, physical, chemical, or physicochemical methods to evacuate the container to create a vacuum. The first switching valve 320 can be a three-way solenoid valve or a valve with multiple channels. The shapes of the first port 321 and the second port 322 can be regular shapes such as circular or polygonal, or irregular. In the embodiments of the present application, to facilitate the formation, manufacture, and / or connection of the first port 321 and the second port 322 to common pipes, the first port 321 and the second port 322 are circular.

[0140] See also Figure 1 、 Figure 11 and Figure 12In some embodiments, the vacuum device 300 further includes a second switching valve 330, which is connected to the first switching valve 320, and the second switching valve 330 is configured to switch between a first working state and a second working state; when the second switching valve 330 is in the first working state, the second switching valve 330 is connected to the vacuum pump 310, so that the vacuum pump 310 can be connected to the channel 203 or the output port 26 through the second switching valve 330 and the first switching valve 320 in sequence; when the second switching valve 330 is in the second working state, the connection between the second switching valve 330 and the vacuum pump 310 is disconnected, and the second switching valve 330 is connected to the external atmosphere, so that the channel 203 or the output port 26 can be connected to the external atmosphere through the first switching valve 320 and the second switching valve 330 in sequence.

[0141] In this way, the second switching valve 330 can connect the channel 203 or the output port 26 to the vacuum pump 310 to adsorb the fluid flow unit 20 on the carrier 210 or absorb the residual liquid attached to the manifold assembly 10; the second switching valve 330 can also connect the channel 203 or the output port 26 to the external atmosphere to facilitate the removal and placement of the fluid flow unit 20.

[0142] Specifically, the second switching valve 330 can be a three-way solenoid valve or a valve with multiple channels. When the second switching valve 330 is in the first operating state and the first switching valve 320 is connected to the channel 203, the channel 203 can be connected to the vacuum pump 310 to adsorb the fluid flow unit 20 on the carrier 210. When the second switching valve 330 is in the first operating state and the first switching valve 320 is connected to the output port 26, the output port 26 can be connected to the vacuum pump 310 to absorb residual liquid attached to the manifold assembly 10. When the second switching valve 330 is in the second operating state, the first switching valve 320 can be connected to the channel 203 or the output port 26, allowing the channel 203 or the output port 26 to be connected to the external atmosphere, thereby removing the fluid flow unit 20 from the carrier 210.

[0143] See also Figure 1 In some embodiments, the second switching valve 330 includes a third port 331, a fourth port 332, and a fifth port 333, and the vacuum device 300 includes a first pipeline 340, a second pipeline 350, and a third pipeline 360. One end of the first pipeline 340 is connected to the third port 331 of the second switching valve 330, and the other end is connected to the first port 321 of the first switching valve 320. The second pipeline 350 and the third pipeline 360 ​​are respectively connected to the fourth port 332 and the fifth port 333 of the second switching valve 330, and the vacuum pump 310 is arranged on the second pipeline 350.

[0144] In this way, by selecting the fourth port 332 and the fifth port 333 , the second switching valve 330 can be switched between the first working state and the second working state.

[0145] Specifically, the shapes of the third port 331, the fourth port 332, and the fifth port 333 can be regular shapes such as circles and polygons, or can be irregular shapes. In the embodiment of the present application, in order to facilitate the formation, manufacture, and / or connection of the third port 331, the fourth port 332, and the fifth port 333 with common pipes, the third port 331, the fourth port 332, and the fifth port 333 are circular.

[0146] The first pipeline 340, the second pipeline 350 and the third pipeline 360 ​​can be pipelines for transmitting fluids, including but not limited to gas, liquid, etc. The cross-sectional shapes of the first pipeline 340, the second pipeline 350 and the third pipeline 360 ​​can be circular, and the length and diameter of the first pipeline 340, the second pipeline 350 and the third pipeline 360 ​​can be set according to actual needs.

[0147] See also Figure 1 In some embodiments, a gas-water separation component 341 is provided on the first pipeline 340 , and the gas-water separation component 341 is used to separate the gas and liquid entering the first pipeline 340 .

[0148] In this way, the gas and liquid entering the first pipeline 340 can be separated by the gas-water separation component 341 to prevent the liquid from entering the vacuum pump 310 , thereby affecting the vacuum adsorption effect and service life of the vacuum pump 310 .

[0149] Specifically, the gas-water separation component 341 may be a vacuum filter with a water separation function. The gas-water separation component 341 may separate the gas and liquid in the first pipeline 340 and store the separated liquid.

[0150] See also Figure 1 In some embodiments, a pressure detection component 342 is provided on the first pipeline 340 .

[0151] In this way, the pressure detecting member 342 can detect the pressure on the first pipeline 340 .

[0152] Specifically, the pressure detection element 342 may be a pressure sensor that can detect the pressure on the first pipeline 340. In some embodiments, a vacuum pressure relief valve 343 may be provided between the pressure detection element 342 and the second switching valve 330. The vacuum pressure relief valve 343 can adjust the opening of the second switching valve 330 based on the detection result of the pressure detection element 342, thereby adjusting the pressure on the first pipeline 340.

[0153] See also Figure 1In some embodiments, a muffler 351 is provided on the second pipeline 350 .

[0154] In this way, the muffler 351 can reduce the exhaust noise of the vacuum pump 310, protect the quality of the working environment of the fluid system 1000, and improve work efficiency.

[0155] Specifically, the silencer 351 can be a device that prevents sound from propagating while allowing air to pass through. The silencer 351 can be made of stainless steel and polyester fiber. The shape of the silencer 351 can be cylindrical. The air inlet of the silencer 351 can be connected to the exhaust port of the vacuum pump 310 so that the noise generated by the vacuum pump 310 can enter the silencer 351 and be weakened.

[0156] See also Figure 1 In some embodiments, an air filter 361 is provided on the third pipeline 360 ​​.

[0157] In this way, the air filter 361 can filter impurities in the gas entering the third pipeline 360 ​​to prevent the impurities from passing through the third pipeline 360 ​​to contaminate the liquid system 1000 , thereby affecting the adsorption performance of the vacuum pump 310 and the sequencing accuracy.

[0158] Specifically, the air filter 361 can filter out dust and solid impurities in the air, and different filter elements can be selected according to actual needs to achieve corresponding filtering accuracy.

[0159] See also Figure 1 In some embodiments, the power assembly 30 includes a syringe pump 31 , which is used to provide power to allow liquid to enter the fluid device 100 and flow back and forth between the manifold assembly 10 and the fluid channel 21 .

[0160] In this way, the syringe pump 31 can provide power to drive the liquid to flow back and forth between the manifold assembly 10 and the fluid channel 21 .

[0161] Specifically, there can be multiple syringe pumps 31, and the multiple syringe pumps 31 are provided in a one-to-one correspondence with the multiple liquid outlets 14. The syringe pump 31 can include a syringe 32 and a single pump. The single pump can include a four-port valve head 33. The single pump is connected to the syringe 32, the second manifold 13, and the waste liquid tank 34 through the four-port valve head 33. The waste liquid tank 34 can store waste liquid flowing through the first manifold 11 and the second manifold 13.

[0162] The syringe pump 31 can operate in both directions, that is, it can drive liquid in both directions as needed. The syringe pump 31 can drive the liquid through the liquid outlet 14, the second liquid hole 45, the flow channel outlet 23, the flow channel inlet 22, and the first liquid hole 41 in sequence to flow into the liquid inlet 12. The syringe pump 31 can also drive the liquid through the liquid inlet 12, the first liquid hole 41, the flow channel inlet 22, the flow channel outlet 23, the second liquid hole 45, and the liquid outlet 14 in sequence to flow into the waste liquid tank 34. Because the fluid flow unit 20 can withstand a large positive pressure, the liquid can clean the relevant pipelines while flowing back and forth between the manifold assembly 10 and the fluid channel 21.

[0163] In one embodiment, the application process of the fluid circuit system 1000 of the embodiment of the present application can be as follows: the positioning pin 25 on the fluid flow unit 20 is plugged into the positioning hole 202 on the carrier 210, so that the manifold assembly 10 is connected to the fluid channel 21, the adsorption hole 206 on the carrier 210 is connected to the first switching valve 320 through the air circuit connector 301, the first pipeline 340 is connected to the first switching valve 320 and the second switching valve 330, and the second switching valve 330 is connected to the vacuum pump 310 on the second pipeline 350, so that the vacuum pump 310 can adsorb and fix the fluid flow unit 20 on the carrier 210. The manifold assembly 10 is connected to the syringe pump 31, and the syringe pump 31 drives the liquid to flow back and forth between the manifold assembly 10 and the fluid channel 21. The liquid fully dissolves the crystals formed by the residue on the manifold assembly 10 and fully mixes and / or replaces the residue on the manifold assembly 10 before flowing into the waste liquid pool 34. The first switching valve 320 is connected to the output port 26 of the fluid flow unit 20 , and the fluid flow unit 20 is moved to disconnect the fluid channel 21 from the manifold assembly 10 . The residual liquid attached to the manifold assembly 10 is sucked away by the vacuum pump 310 .

[0164] See also Figure 15 The present application provides a method for controlling the flow of liquid in a fluid circuit system 1000, the method comprising:

[0165] a), connecting the manifold assembly 10 with the fluid channel 21;

[0166] b) using the power assembly 30 to drive the liquid into the fluid channel 21 through the manifold assembly 10;

[0167] c) using the power assembly 30 to drive the liquid from the fluid channel 21 back to the manifold assembly 10;

[0168] Repeat steps b) and c).

[0169] In this way, through the above steps, the liquid can flow back and forth between the manifold assembly 10 and the fluid channel 21, which can fully mix and / or replace the residues on the manifold assembly 10, and can also fully dissolve the crystals formed by the residues remaining on the manifold assembly 10, thereby facilitating the cleaning of the manifold assembly 10 and improving the sequencing effect.

[0170] Specifically, in step a), the fluid flow unit 20 may be adsorbed onto the carrying surface 201 by the vacuum pump 310 , thereby connecting the manifold assembly 10 with the fluid channel 21 .

[0171] In step b), the power assembly 30 may include a syringe pump 31 , which may drive the liquid to flow into the fluid channel 21 through the manifold assembly 10 .

[0172] In step c), the power assembly 30 may include a syringe pump 31 , which may drive the liquid from the fluid channel 21 back to the manifold assembly 10 .

[0173] See also Figure 16 In certain embodiments, the method comprises:

[0174] d) moving the fluid flow unit 20 to disconnect the fluid channel 21 from the manifold assembly 10 , and connecting the fluid channel 21 to the vacuum device 300 ;

[0175] e) using the vacuum device 300 to create a negative pressure between the manifold assembly 10 and the fluid channel 21 to suck away the residual liquid attached to the manifold assembly 10 through the fluid channel 21;

[0176] d) and e) are performed after a), b) and c) are completed.

[0177] Thus, through the above steps, the residual liquid adhering to the manifold assembly 10 can be sucked away. d) After the completion of a), b), and c), it is beneficial to prevent the residue and / or crystals formed by the residue from clogging the pipeline and affecting the adsorption effect and service life of the vacuum device 300.

[0178] Specifically, the fluid flow unit 20 can be moved to disconnect the fluid channel 21 from the manifold assembly 10, for example, by driving the fluid flow unit 20 to move away from the carrier 210 along the thickness direction of the fluid flow unit 20. The manifold assembly 10 can also be moved to disconnect the fluid channel 21 from the manifold assembly 10, for example, by driving the manifold assembly 10 to move away from the fluid flow unit 20 along the thickness direction of the fluid flow unit 20.

[0179] Combine Figure 14In some embodiments, a sealing ring 207 is provided in the positioning hole 202 of the carrier 210, and the positioning pin 25 of the fluid flow unit 20 is inserted into the sealing ring 207. The fluid flow unit 20 can move 1mm-3mm relative to the carrier 210 to prevent the positioning pin 25 from being separated from the sealing ring 207.

[0180] An embodiment of the present application provides a sequencing system, which includes a liquid circuit system 1000.

[0181] In the sequencing system of the present embodiment, the fluidic system 1000, via the power assembly 30, drives the liquid back and forth between the manifold assembly 10 and the fluid channel 21. This allows for thorough mixing and / or replacement of residues on the manifold assembly 10, as well as for thorough redissolution of crystals formed by residues on the manifold assembly 10, thereby facilitating cleaning of the manifold assembly 10 and improving sequencing performance. Sequencing systems include, but are not limited to, nucleic acid sequence determination systems.

[0182] An embodiment of the present application provides a non-volatile computer-readable storage medium storing a computer program. When the computer program is executed by one or more processors, the method of any of the above embodiments is implemented.

[0183] Specifically, in one embodiment, the processor may be a central processing unit (CPU). The processor may also be other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIS30), field-programmable gate arrays (FPGS10), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, or a combination of the above chips.

[0184] Computer programs can be stored in a memory. Memory, as a non-transitory computer-readable storage medium, can be used to store non-transitory software programs, non-transitory computer executable programs, and modules, such as the program instructions / modules corresponding to the methods in the above-described method embodiments. The processor executes the non-transitory software programs, instructions, and modules stored in the memory to perform various functional applications and data processing of the processor, thereby implementing the methods in the above-described method embodiments.

[0185] The storage medium may include, but is not limited to, various media that can store computer programs, such as a USB flash drive, a read-only memory (ROM), a random access memory (RAM), a mobile hard disk, a magnetic disk, or an optical disk.

[0186] Throughout this specification, references to terms such as "one embodiment," "certain embodiments," and the like indicate that a particular feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0187] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the said features. In the description of the present invention, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.

[0188] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections, or mutual communication; direct connections or indirect connections through an intermediate medium; and internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0189] In the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Furthermore, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0190] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to the embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the claims and their equivalents.

Claims

1. A fluid system, characterized in that: include: A fluid device, comprising a manifold assembly, a fluid flow unit, and a power assembly, wherein the fluid flow unit comprises at least one fluid channel, the fluid flow unit is connected to the manifold assembly through the fluid channel, the power assembly is connected to the fluid flow unit, and the power assembly is used to provide power to enable liquid to enter the fluid device and flow back and forth between the manifold assembly and the fluid channel.

2. The fluid circuit system according to claim 1, characterized in that: The manifold assembly includes a first manifold member and a second manifold member, the first manifold member is provided with at least one liquid inlet, the second manifold member is provided with at least one liquid outlet, and the liquid inlet and the liquid outlet are provided in a one-to-one correspondence; The fluid flow unit is located between the first manifold member and the second manifold member; Each of the fluid channels includes a channel inlet and a channel outlet; The first manifold is in communication with the fluid channel via the liquid inlet and the flow channel inlet; The second manifold is in communication with the fluid channel via the liquid outlet and the flow channel outlet; Optionally, the flow channel inlet and the flow channel outlet each have a first central axis, the liquid inlet and the liquid outlet each have a second central axis, the first central axis of the flow channel inlet is offset from the second central axis of the liquid inlet, and the first central axis of the flow channel outlet is offset from the second central axis of the liquid outlet; Optionally, a first sealing gasket is provided between the first manifold and the fluid flow unit, and a second sealing gasket is provided between the second manifold and the fluid flow unit, the first sealing gasket is provided with at least one first liquid hole, the second sealing gasket is provided with at least one second liquid hole, the first liquid holes are provided in a one-to-one correspondence with the liquid inlets, the second liquid holes are provided in a one-to-one correspondence with the liquid outlets, the liquid inlet is connected to the flow channel inlet through the first liquid hole, and the liquid outlet is connected to the flow channel outlet through the second liquid hole; Optionally, the first liquid hole and the second liquid hole each have a third central axis, and the third central axis is coaxially arranged with the second central axis; Optionally, the first sealing gasket includes a first gasket body and a first flange, the first liquid hole is provided on the first gasket body, and the first flange is provided around the first liquid hole; The second sealing gasket includes a second gasket body and a second flange. The second liquid hole is provided on the second gasket body, and the second flange is provided around the second liquid hole.

3. The fluid circuit system according to claim 1 or 2, characterized in that: The fluid system comprises: stage device; The manifold assembly and the fluid flow unit are arranged on the stage device, and the fluid flow unit is detachably connected to the stage device; Optionally, the stage device includes a carrying platform, the carrying platform has a carrying surface, and the fluid flow unit is located on the carrying surface; Optionally, a positioning hole is provided on the bearing surface, and a positioning pin is provided on the fluid flow unit, and the positioning hole and the positioning pin are plugged and matched, so that the fluid flow unit can be detachably mounted on the bearing surface; Optionally, a groove is provided inwardly on the bearing surface; Optionally, the channel includes at least one first channel and at least one second channel, and the first channel and the second channel are connected to each other; Optionally, the first channel and the second channel are arranged to cross each other; Optionally, there are multiple first grooves, and the multiple first grooves are spaced apart and arranged in parallel; there are multiple second grooves, and the multiple second grooves are spaced apart and arranged in parallel; the first grooves and the second grooves are perpendicular to each other.

4. The fluid circuit system according to any one of claims 1 to 3, characterized in that: include: Vacuum device; The fluid flow unit is provided with an output port connected to the fluid channel; The vacuum device is in communication with the fluid channel via the output port; Optionally, there are multiple fluid channels, and the output ports on the multiple fluid channels are connected to the vacuum device; Optionally, the vacuum device includes a vacuum pump and a first switching valve; The first switching valve has a first port and two second ports; The first switching valve is connected to the vacuum pump through the first port; The first switching valve is connected to the channel through one of the second ports; The first switching valve is connected to the output port through another second port; Optionally, the vacuum device further comprises a second switching valve, the second switching valve being in communication with the first switching valve, the second switching valve being configured to switch between a first working state and a second working state; When the second switching valve is in the first working state, the second switching valve is connected to the vacuum pump, so that the vacuum pump can be connected to the channel or the output port through the second switching valve and the first switching valve in sequence; When the second switching valve is in a second working state, the second switching valve is disconnected from the vacuum pump and is connected to the outside atmosphere, so that the channel or the output port can be connected to the outside atmosphere through the first switching valve and the second switching valve in sequence; Optionally, the second switching valve includes a third port, a fourth port, and a fifth port; The vacuum device includes a first pipeline, a second pipeline and a third pipeline; One end of the first pipeline is connected to the third port of the second switching valve, and the other end is connected to the first port of the first switching valve; The second pipeline and the third pipeline are connected to the fourth port and the fifth port of the second switching valve respectively; The vacuum pump is arranged on the second pipeline; Optionally, a gas-water separation component is provided on the first pipeline, and the gas-water separation component is used to separate the gas and liquid entering the first pipeline; Optionally, a pressure detection component is provided on the first pipeline; Optionally, a muffler is provided on the second pipeline; Optionally, an air filter is provided on the third pipeline; Optionally, the power assembly comprises a syringe pump, and the syringe pump is used to provide power to allow liquid to enter the fluid device and flow back and forth between the manifold assembly and the fluid channel.

5. A method for controlling the flow of liquid in a liquid circuit system, characterized in that: The fluid system comprises: A fluid device, the fluid device comprising a manifold assembly, a fluid flow unit, and a power assembly, the fluid flow unit comprising at least one fluid channel, the power assembly being connected to the fluid flow unit; The method comprises: a) connecting the manifold assembly with the fluid channel; b) using the power assembly to drive the liquid into the fluid channel through the manifold assembly; c) driving the liquid from the fluid channel back to the manifold assembly using the power assembly; Repeat steps b) and c).

6. The method according to claim 5, characterized in that The method comprises: d) moving the fluid flow unit to disconnect the fluid channel from the manifold assembly and connect the fluid channel to a vacuum device; e) using the vacuum device to generate negative pressure between the manifold assembly and the fluid channel to suck away residual liquid attached to the manifold assembly through the fluid channel; The above d) and e) are carried out after the above a), b) and c) are completed.

7. The method according to claim 5 or 6, characterized in that include: The manifold assembly includes a first manifold member and a second manifold member, the first manifold member is provided with at least one liquid inlet, the second manifold member is provided with at least one liquid outlet, and the liquid inlet and the liquid outlet are provided in a one-to-one correspondence; The fluid flow unit is located between the first manifold member and the second manifold member; Each of the fluid channels includes a channel inlet and a channel outlet; The first manifold is in communication with the fluid channel via the liquid inlet and the flow channel inlet; The second manifold is in communication with the fluid channel via the liquid outlet and the flow channel outlet; Optionally, the flow channel inlet and the flow channel outlet each have a first central axis, the liquid inlet and the liquid outlet each have a second central axis, the first central axis of the flow channel inlet is offset from the second central axis of the liquid inlet, and the first central axis of the flow channel outlet is offset from the second central axis of the liquid outlet; Optionally, a first sealing gasket is provided between the first manifold and the fluid flow unit, and a second sealing gasket is provided between the second manifold and the fluid flow unit, the first sealing gasket is provided with at least one first liquid hole, the second sealing gasket is provided with at least one second liquid hole, the first liquid holes are provided in a one-to-one correspondence with the liquid inlets, the second liquid holes are provided in a one-to-one correspondence with the liquid outlets, the liquid inlet is connected to the flow channel inlet through the first liquid hole, and the liquid outlet is connected to the flow channel outlet through the second liquid hole; Optionally, the first liquid hole and the second liquid hole each have a third central axis, and the third central axis is coaxially arranged with the second central axis; Optionally, the first sealing gasket includes a first gasket body and a first flange, the first liquid hole is provided on the first gasket body, and the first flange is provided around the first liquid hole; The second sealing gasket includes a second gasket body and a second flange, the second liquid hole is provided on the second gasket body, and the second flange is provided around the second liquid hole; Optionally, the fluid system includes a stage device; The manifold assembly and the fluid flow unit are arranged on the stage device, and the fluid flow unit is detachably connected to the stage device; Optionally, the stage device includes a carrying platform, the carrying platform has a carrying surface, and the fluid flow unit is located on the carrying surface; Optionally, a positioning hole is provided on the bearing surface, and a positioning pin is provided on the fluid flow unit, and the positioning hole and the positioning pin are plugged and matched, so that the fluid flow unit can be detachably mounted on the bearing surface; Optionally, a groove is provided inwardly on the bearing surface; Optionally, the channel includes at least one first channel and at least one second channel, and the first channel is connected to the second channel; Optionally, the first channel and the second channel are arranged to cross each other; Optionally, there are multiple first grooves, and the multiple first grooves are spaced apart and arranged in parallel; there are multiple second grooves, and the multiple second grooves are spaced apart and arranged in parallel; the first grooves and the second grooves are perpendicular to each other.

8. The method according to any one of claims 5 to 7, characterized in that: include: Vacuum device; The fluid flow unit is provided with an output port connected to the fluid channel; The vacuum device is in communication with the fluid channel via the output port; Optionally, the fluid channels include a plurality of channels, and the output ports on the plurality of fluid channels are connected to the vacuum device; Optionally, the vacuum device includes a vacuum pump and a first switching valve; The first switching valve has a first port and two second ports; The first switching valve is connected to the vacuum pump through the first port; The first switching valve is connected to the channel through one of the second ports; The first switching valve is connected to the output port through another second port; Optionally, the vacuum device further comprises a second switching valve, the second switching valve being in communication with the first switching valve, the second switching valve being configured to switch between a first working state and a second working state; When the second switching valve is in the first working state, the second switching valve is connected to the vacuum pump, so that the vacuum pump can be connected to the channel or the output port through the second switching valve and the first switching valve in sequence; When the second switching valve is in a second working state, the second switching valve is disconnected from the vacuum pump and is connected to the outside atmosphere, so that the channel or the output port can be connected to the outside atmosphere through the first switching valve and the second switching valve in sequence; Optionally, the second switching valve includes a third port, a fourth port, and a fifth port; The vacuum device includes a first pipeline, a second pipeline and a third pipeline; One end of the first pipeline is connected to the third port of the second switching valve, and the other end is connected to the first port of the first switching valve; The second pipeline and the third pipeline are connected to the fourth port and the fifth port of the second switching valve respectively; The vacuum pump is arranged on the second pipeline; Optionally, a gas-water separation component is provided on the first pipeline, and the gas-water separation component is used to separate the gas and liquid entering the first pipeline; Optionally, a pressure detection component is provided on the first pipeline; Optionally, a muffler is provided on the second pipeline; Optionally, an air filter is provided on the third pipeline; Optionally, the power assembly comprises a syringe pump, and the syringe pump is used to provide power to allow liquid to enter the fluid device and flow back and forth between the manifold assembly and the fluid channel.

9. A sequencing system, characterized in that The invention comprises the fluid circuit system according to any one of claims 1 to 4.

10. A non-volatile computer-readable storage medium storing a computer program, characterized in that: When the computer program is executed by one or more processors, the method according to any one of claims 5 to 8 is implemented.

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