Enzymolysis device and method for polyphyllin extraction process

Abstract

The invention is applicable to the technical field of enzymolysis devices, and provides an enzymolysis device and method for a polyphyllin extraction process, and the enzymolysis device comprises a shell and an inner container which is arranged in the shell and is used for enzymolysis of a polyphyllin raw material; the connecting rod is arranged in the inner container in a sliding mode, and a transverse plate used for stirring raw materials in an up-down reciprocating mode is fixedly installed at the bottom end of the connecting rod; the protective cover is fixedly mounted at the top of the shell; the threaded cylinder is rotationally mounted at the top of the protective cover; the first threaded rod is arranged on the threaded cylinder in a threaded and sleeving mode and detachably connected with the connecting rod, and the first threaded rod is used for driving the connecting rod to move up and down. According to the enzymolysis device and method for the polyphyllin extraction process, by adopting a multi-mode stirring mode combining vertical moving stirring of the transverse plate, peristaltic stirring and rotation of the inner container, damage of mechanical shear force to enzyme activity is avoided, and the enzymolysis reaction efficiency and the saponin extraction rate are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of enzymatic hydrolysis devices, and in particular relates to an enzymatic hydrolysis device and method for a Paris polyphylla saponin extraction process. Background Art

[0002] As a traditional Chinese medicinal material, Paris polyphylla contains a variety of biologically active ingredients. Among them, Paris polyphylla saponins have attracted much attention due to their significant anti-tumor, anti-inflammatory, and antibacterial pharmacological effects. In the extraction process of Paris polyphylla saponins, enzymatic hydrolysis technology has been widely used as an important auxiliary means. Enzymatic hydrolysis technology can decompose the Paris polyphylla cell walls or intracellular components through specific enzymes, thereby releasing more saponins and improving the extraction rate and purity of saponins.

[0003] At present, in the extraction process of Paris polyphylla saponins and enzymatic hydrolysis reactions in other biochemical fields, most of the enzymatic hydrolysis devices used are equipped with a stirring mechanism to promote sufficient contact and reaction between the enzyme and the substrate. Among them, mechanical stirring is the most common stirring method. During the stirring process, the high-speed rotation of the stirring paddle will generate a strong mechanical shear force. This shear force will have a direct physical effect on the enzyme molecules, causing the structure of the enzyme molecules to twist, deform or even break. Especially for some enzymes with relatively fragile structures, mechanical shear force is more likely to destroy the structure of their active sites, causing the enzyme to lose its catalytic activity. Summary of the Invention

[0004] The present invention provides an enzymatic hydrolysis device and method for a Paris polyphylla saponin extraction process, aiming to solve the problem in the above background technology that traditional mechanical stirring easily leads to enzyme activity loss.

[0005] To solve the above problems, the present invention is implemented as follows: an enzymatic hydrolysis device for a Paris polyphylla saponin extraction process, comprising: a shell and an inner liner arranged in the shell for enzymatic hydrolysis of Paris polyphylla saponin raw materials; a connecting rod slidably arranged in the inner liner, the bottom end of the connecting rod being fixedly provided with a horizontal plate for reciprocatingly stirring the raw materials up and down; a protective cover fixedly mounted on the top of the shell; a threaded barrel rotatably mounted on the top of the protective cover; a first threaded rod threadedly sleeved on the threaded barrel and detachably connected to the connecting rod, the first threaded rod being used to drive the connecting rod to move up and down; and a driving mechanism arranged on the protective cover for driving the threaded barrel to rotate.

[0006] Preferably, the driving mechanism includes: a motor fixedly mounted on one side of the protective cover; a differential wheel fixedly mounted on the motor output shaft and the threaded barrel respectively; and a belt mounted on the differential wheel.

[0007] Preferably, the inner liner consists of a silicone shell, a top plate, a bottom plate and an annular sleeve, the top plate is installed on the top of the silicone shell, the bottom plate is provided on the silicone shell, the annular sleeve is rotatably mounted on the bottom plate, and the annular sleeve is fixedly connected to the silicone shell.

[0008] Preferably, a discharge pipe is installed at the bottom of the base plate, and a conical plate is fixedly sleeved on the discharge pipe, and the conical plate is adapted to the conical notch at the bottom of the shell. A support ball is fixedly installed at the bottom of the inner wall of the shell, and the support ball is in contact with the base plate. A support block is fixedly installed on one side of the inner wall of the shell, and a support wheel is fixedly installed on the top of the support block, and the support wheel is in close contact with the bottom of the top plate.

[0009] Preferably, a jacket is installed in the shell, and the material of the jacket is high thermal conductivity stainless steel. Two temperature sensors for monitoring the temperature of the shell and the inner tank are fixedly installed in the shell and the inner tank.

[0010] Preferably, the shell is provided with a rotating mechanism for driving the inner liner to rotate, and the rotating mechanism includes: a ring gear fixedly mounted on the top of the inner liner; a driven gear rotatably mounted on the top of the inner wall of the shell, the driven gear meshing with the ring gear; a driving gear fixedly sleeved on the driven gear shaft; a hydraulic cylinder mounted on the top of the inner wall of the shell, a first rack fixedly mounted on the hydraulic cylinder, and the first rack meshing with the driving gear.

[0011] Preferably, a feed pipe is slidably installed on the top of the shell, a bellows is installed on the top of the feed pipe, annular pressure plates are fixedly installed on the top of the inner tank and the bottom end of the feed pipe, annular sealing gaskets are installed on both of the annular pressure plates, and the two annular sealing gaskets are in close contact.

[0012] Preferably, a height adjustment mechanism for adjusting the height of the feed pipe is provided in the shell, and the height adjustment mechanism includes: a second threaded rod rotatably installed on the top of the inner wall of the shell; a slider threadedly sleeved on the second threaded rod, and the slider is fixedly connected to the annular pressure plate located on the feed pipe; a first gear fixedly sleeved on the second threaded rod; a second rack installed on one side of the first rack, and the second rack is meshed with the first gear.

[0013] Preferably, a guide rod is fixedly installed on the top of the shell, a connecting plate is slidably sleeved on the guide rod, the connecting plate is fixedly connected to the first threaded rod, and a hanging ring is fixedly installed on the top cover of the shell, and the hanging ring is used to hoist the top cover.

[0014] The present invention also provides an enzymatic hydrolysis method for an enzymatic hydrolysis device used in a Paris polyphylla saponin extraction process, comprising the following steps: Step 1: Add the Paris polyphylla saponin raw material into the feed pipe through the bellows, and the raw material enters the inner container through the bottom of the feed pipe. After all the materials are poured in, start the motor, and the motor output shaft drives the differential wheel to rotate. Through the belt transmission, the differential wheel on the threaded barrel rotates, and then drives the threaded barrel to rotate. The first threaded rod moves up and down under the rotation of the threaded barrel, driving the connecting rod to move up and down in the inner container, and the horizontal plate moves up and down in a small range accordingly, moving the lower layer of raw materials upward to achieve preliminary stirring; Step 2: The hydraulic cylinder drives the first rack to move, driving the driving gear to rotate, and then drives the driven gear to rotate. The driven gear meshes with the ring gear, driving the inner pot to rotate. The rotation angle of the inner pot is controlled at 20 to 40 degrees, further changing the position and distribution of the material to make the mixing more uniform. Step 3: As the first rack moves, the second rack drives the first gear to rotate, which in turn drives the second threaded rod to rotate. The slider moves upward under the rotation of the second threaded rod, driving the feed pipe upward to maintain a safe distance between the feed pipe and the top of the inner container, preventing the inner container from colliding with the feed pipe or wearing the annular sealing gasket during subsequent rotation; Step 4: Hot water enters the jacket through the pipe. The high thermal conductivity stainless steel jacket quickly transfers heat to the surrounding environment of the inner tank, causing the material in the inner tank to heat up. Temperature sensors in the shell and inner tank monitor the temperature in real time. The operator adjusts the hot water flow rate, temperature and other parameters based on the temperature information to ensure that the enzymatic hydrolysis reaction proceeds within the appropriate temperature range. Step 5: After the enzymatic hydrolysis reaction is completed, the material is discharged from the discharge pipe. When the inner liner needs to be replaced, since the inner liner is firmly fixed in the shell by the support balls and support wheels and is not fixedly connected to the shell, the inner liner can be lifted out of the shell for replacement by lifting equipment. After replacing the new inner liner, follow the above steps to repeat the feeding, enzymatic hydrolysis and stirring operations.

[0015] Compared with the related art, the enzymatic hydrolysis device and method for extracting parsnip saponins provided by the present invention have the following beneficial effects: Compared with existing technologies, the enzymatic hydrolysis device and method for extracting Paris polyphylla saponins provided in this solution utilizes a peristaltic stirring mechanism with a horizontal plate that moves up and down within a small range. This prevents mechanical shear forces from damaging the enzyme structure, helps maintain enzyme activity, and improves the efficiency of the enzymatic hydrolysis reaction, thereby releasing more saponins and further increasing the extraction rate and purity of the saponins. Compared with mechanical stirring, this stirring method maintains enzyme activity while being more conducive to the extraction process.

[0016] In summary, the enzymatic hydrolysis device and method for the extraction of Paris polyphylla saponins of the present invention avoids the destruction of enzyme activity by mechanical shear force by adopting a multi-mode stirring method combining up and down movement of the horizontal plate, peristaltic stirring and rotation of the inner tank, thereby improving the enzymatic hydrolysis reaction efficiency and saponin extraction rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the main structure of an enzymatic hydrolysis device for extracting Paris polyphylla saponins provided by the present invention; Figure 2 This is a schematic diagram of the main cross-sectional structure of an enzymatic hydrolysis device for a Paris polyphylla saponin extraction process provided by the present invention; Figure 3 This is a schematic diagram of a top-down cross-sectional structure of an enzymatic hydrolysis device for a Paris polyphylla saponin extraction process provided by the present invention; Figure 4 It is a schematic top view of the ring gear and the driven gear provided by the present invention; Figure 5 1 is a schematic top view of the driving gear and the first rack provided by the present invention; Figure 6 This is an assembly diagram of the annular pressure plate and the annular sealing gasket provided by the present invention; Figure 7 It is a schematic top view of the structure of the first sprocket, the limiting wheel and the first chain provided by the present invention; Figure 8 This is an assembly diagram of the first threaded rod, the guide rod, and the connecting plate provided by the present invention; Figure 9 This is a schematic diagram of the main structure of the linkage mechanism provided by the present invention; Figure 10 for Figure 2 Schematic diagram of the enlarged structure of part A shown in FIG; Figure 11 for Figure 2 Schematic diagram of the enlarged structure of part B shown in FIG; Figure 12 for Figure 3 Schematic diagram of the enlarged structure of part C shown in ; Figure 13 for Figure 2 Schematic diagram of the enlarged structure of part D shown in FIG; Figure 14 for Figure 13 Schematic diagram of the enlarged structure of part E shown in FIG.

[0018] Figure numerals: 1, shell; 2, liner; 201, silicone shell; 202, top plate; 203, bottom plate; 204, annular sleeve; 3, connecting rod; 4, cross plate; 5, protective cover; 6, threaded barrel; 7, first threaded rod; 8, motor; 9, differential wheel; 10, belt; 11, tapered plate; 12, support ball; 13, jacket; 14, temperature sensor; 15, ring gear; 16, driven gear; 17, driving gear; 18, hydraulic cylinder; 19, first rack; 20, feed pipe; 21, bellows; 22, annular pressure plate; 23, annular sealing gasket; 24, second threaded rod; 25, slider; 26, first gear; 27, second rack; 28, sleeve; 29, annular scraper; 30, sealing ring; 31, guide rod; 32, connecting plate; 33, hanging Ring; 34. Connecting box; 35. One-way screw; 36. Connecting block; 37. Rectangular telescopic rod; 38. Connecting frame; 39. Roller; 40. Mounting plate; 41. Toothless gear; 42. Third rack; 43. Fourth rack; 44. Second gear; 45. Ring box; 46. First sprocket; 47. Limiting wheel; 48. First chain; 49. First rotating rod; 50. Second rotating rod; 51. Second sprocket; 52. Second chain; 53. Round rod; 54. Bevel gear; 55. Rectangular seat; 56. Rectangular rod; 57. Collecting box; 58. Bracket; 59. Connecting rod; 60. Sealing block; 61. Spring; 62. Support plate; 63. Switch device; 64. Elastic steel plate; 65. Third threaded rod; 66. Rectangular cylinder; 67. Support block; 68. Support wheel. DETAILED DESCRIPTION

[0019] References herein to "embodiments" mean that a particular feature, structure, or characteristic described in connection with the embodiments may be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor does it constitute an independent or alternative embodiment that is mutually exclusive of other embodiments. It is understood, both explicitly and implicitly, by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0020] The embodiment of the present invention provides an enzymatic hydrolysis device and method for extracting parsnip saponins. Figure 1-14 As shown, the enzymatic hydrolysis device for the extraction process of Paris polyphylla saponins includes: a shell 1 and an inner liner 2 arranged in the shell 1 for enzymatic hydrolysis of Paris polyphylla saponin raw materials; a connecting rod 3 slidably arranged in the inner liner 2, and the bottom end of the connecting rod 3 is fixedly installed with a horizontal plate 4 for reciprocating stirring of the raw materials up and down; a protective cover 5 fixedly installed on the top of the shell 1; a threaded barrel 6 rotatably installed on the top of the protective cover 5; a first threaded rod 7 threadedly sleeved on the threaded barrel 6 and detachably connected to the connecting rod 3, the first threaded rod 7 being used to drive the connecting rod 3 to move up and down; and a driving mechanism arranged on the protective cover 5 for driving the threaded barrel 6 to rotate.

[0021] In this embodiment, during operation, the driving mechanism drives the threaded barrel 6 to rotate. Since the first threaded rod 7 is threadedly sleeved on the threaded barrel 6 and detachably connected to the connecting rod 3, the rotation of the threaded barrel 6 will drive the first threaded rod 7 to move up and down, thereby driving the connecting rod 3 to move up and down in the inner tank 2. The horizontal plate 4 fixed at the bottom end of the connecting rod 3 moves up and down in a small range, moving the Paris polyphylla saponin raw material located in the lower layer upward. At the same time, the peristaltic stirring mechanism equipped with the device operates synchronously, imitating intestinal peristalsis to perform peristaltic operation on the inner tank 2. The strong mechanical shear force generated by the high-speed rotation of the stirring paddle in traditional mechanical stirring will destroy the enzyme molecular structure, causing the enzyme to lose its catalytic activity. However, the present device avoids the damage of the enzyme structure to the mechanical shear force by combining the peristaltic stirring mechanism with the up and down small range movement of the horizontal plate 4, which is beneficial to maintaining the activity of the enzyme and improving the efficiency of the enzymatic hydrolysis reaction. The position of the raw material changes by the up and down movement of the horizontal plate 4, and the peristaltic operation of the peristaltic stirring mechanism enhances the contact and mixing between the solid and liquid, improves the solid-liquid mass transfer efficiency, thereby releasing more saponins and further improving the extraction rate and purity of saponins. Compared with mechanical stirring, this stirring method is more conducive to the extraction process while ensuring enzyme activity.

[0022] In a further preferred embodiment of the present invention, the driving mechanism includes: a motor 8 fixedly mounted on one side of the protective cover 5; a differential wheel 9 fixedly mounted on the output shaft of the motor 8 and the threaded cylinder 6 respectively; and a belt 10 mounted on the differential wheel 9.

[0023] In this embodiment, when the motor 8 is started, the output shaft of the motor 8 drives the differential wheel 9 thereon to rotate. Through the transmission action of the belt 10, the other differential wheel 9 fixed on the threaded barrel 6 also rotates, thereby driving the threaded barrel 6 to rotate. The rotation of the threaded barrel 6 will drive the first threaded rod 7 to move up and down, and the first threaded rod 7 will drive the connecting rod 3 to move up and down in the inner tank 2. Through the transmission of the differential wheel 9 and the belt 10, the motor 8 can accurately control the rotation speed and direction of the threaded barrel 6, thereby realizing precise control of the up and down movement of the connecting rod 3 and the cross plate 4, ensuring that the stirring process is carried out in a predetermined manner, which is conducive to improving the effect of the enzymatic hydrolysis reaction.

[0024] In a further preferred embodiment of the present invention, the inner liner 2 is composed of a silicone shell 201, a top plate 202, a bottom plate 203 and an annular sleeve 204, the top plate 202 is installed on the top of the silicone shell 201, the bottom plate 203 is provided on the silicone shell 201, the annular sleeve 204 is rotatably mounted on the bottom plate 203, and the annular sleeve 204 is fixedly connected to the silicone shell 201.

[0025] In this embodiment, the peristaltic stirring mechanism imitates intestinal peristalsis to perform peristaltic operation on the inner liner 2. Since the silicone shell 201 is soft and deformable, it can adapt well to the action of the peristaltic stirring mechanism. At the same time, the driving mechanism cooperates with the peristaltic stirring mechanism to complete the enzymatic hydrolysis and stirring process. Through the setting of the silicone shell 201, it can undergo corresponding deformation as the peristaltic stirring mechanism moves.

[0026] In a further preferred embodiment of the present invention, a discharge pipe is installed at the bottom of the base plate 203, and a conical plate 11 is fixedly sleeved on the discharge pipe, and the conical plate 11 is adapted to the conical notch at the bottom of the shell 1, and a support ball 12 is fixedly installed at the bottom of the inner wall of the shell 1, and the support ball 12 is in contact with the base plate 203, and a support block 67 is fixedly installed on one side of the inner wall of the shell 1, and a support wheel 68 is fixedly installed on the top of the support block 67, and the support wheel 68 is in close contact with the bottom of the top plate 202.

[0027] In this embodiment, when the enzymatic hydrolysis reaction is completed and the material needs to be discharged, the material is discharged from the discharge pipe. When the inner liner 2 needs to be replaced later, since the conical plate 11 is adapted to the conical notch at the bottom of the shell 1, and the inner liner 2 is firmly fixed in the shell 1 by the support balls 12 and the support wheels 68, this design makes the inner liner 2 not fixedly connected to the shell 1, and has space for convenient operation, which facilitates the subsequent lifting of the inner liner 2 from the shell 1 for replacement by lifting equipment.

[0028] In a further preferred embodiment of the present invention, a jacket 13 is installed in the shell 1, and the material of the jacket 13 is high thermal conductivity stainless steel. Two temperature sensors 14 for monitoring the temperature in the shell 1 and the inner tank 2 are fixedly installed in the shell 1 and the inner tank 2.

[0029] In this embodiment, hot water enters the jacket 13 through a pipe. Since high thermal conductivity stainless steel has good thermal conductivity, the heat in the hot water can be quickly transferred to the environment around the inner liner 2 through the jacket 13. The material in the inner liner 2 absorbs this heat to achieve temperature increase, providing suitable temperature conditions for the enzymatic hydrolysis reaction. At the same time, temperature sensors 14 are fixedly installed in the shell 1 and the inner liner 2. These two temperature sensors 14 are respectively used to monitor the temperature in the shell 1 and the inner liner 2 in real time. During the enzymatic hydrolysis reaction, the operator can adjust the flow rate, temperature and other parameters of the hot water in time according to the temperature information fed back by the temperature sensor 14 to ensure that the enzymatic hydrolysis reaction is carried out within the appropriate temperature range.

[0030] In a further preferred embodiment of the present invention, a rotating mechanism for driving the inner liner 2 to rotate is provided on the shell 1, and the rotating mechanism includes: a ring gear 15 fixedly mounted on the top of the inner liner 2; a driven gear 16 rotatably mounted on the top of the inner wall of the shell 1, and the driven gear 16 is engaged with the ring gear 15; a driving gear 17 fixedly sleeved on the rotating shaft of the driven gear 16; a hydraulic cylinder 18 mounted on the top of the inner wall of the shell 1, and a first rack 19 is fixedly mounted on the hydraulic cylinder 18, and the first rack 19 is engaged with the driving gear 17.

[0031] In this embodiment, while the driving mechanism drives the transverse plate 4 to move up and down for stirring operation, the rotating mechanism can be started, and the hydraulic cylinder 18 drives the first rack 19 to move. Since the first rack 19 is engaged with the driving gear 17, it will drive the driving gear 17 to rotate. The rotation of the driving gear 17 drives the driven gear 16 to rotate, and the driven gear 16 is engaged with the ring gear 15, thereby driving the ring gear 15 to rotate, and finally the rotation operation of the inner liner 2 is realized. The rotation angle of the inner liner 2 is controlled at 20 to 40 degrees. On the basis of the transverse plate 4 moving up and down to stir the material, the rotation of the inner liner 2 can further change the position and distribution of the material. The material will have more sufficient contact with the transverse plate during the rotation process, making the stirring more uniform and avoiding local material accumulation or insufficient stirring.

[0032] In a further preferred embodiment of the present invention, a feed pipe 20 is slidably installed on the top of the shell 1, a bellows 21 is installed on the top of the feed pipe 20, and an annular pressure plate 22 is fixedly installed on the top of the inner tank 2 and the bottom end of the feed pipe 20. An annular sealing gasket 23 is installed on both of the annular pressure plates 22, and the two annular sealing gaskets 23 are in close contact.

[0033] In this embodiment, when the material feeding operation is performed, the material enters the feed pipe 20 through the bellows 21, and then enters the inner tank 2 through the bottom end of the feed pipe 20. Since the two annular sealing gaskets 23 are in close contact, a sealing barrier is formed, which can prevent the material from overflowing from the gap between the top of the inner tank 2 and the bottom end of the feed pipe 20.

[0034] In a further preferred embodiment of the present invention, a height adjustment mechanism for adjusting the height of the feed pipe 20 is provided in the shell 1, and the height adjustment mechanism includes: a second threaded rod 24 rotatably mounted on the top of the inner wall of the shell 1; a slider 25 threadedly sleeved on the second threaded rod 24, and the slider 25 is fixedly connected to the annular pressure plate 22 located on the feed pipe 20; a first gear 26 fixedly sleeved on the second threaded rod 24; a second rack 27 installed on one side of the first rack 19, and the second rack 27 is meshed with the first gear 26.

[0035] In this embodiment, when the rotating mechanism is running, the hydraulic cylinder 18 drives the first rack 19 to move. At the same time, the first rack 19 drives the second rack 27 to move. Since the second rack 27 is engaged with the first gear 26, the movement of the second rack 27 will drive the first gear 26 to rotate, and then drive the second threaded rod 24 to rotate. As the second threaded rod 24 rotates, the slider 25 threaded thereon will move upward along the second threaded rod 24, and the movement of the slider 25 will drive the feed pipe 20 to move upward, thereby preventing the annular sealing gasket 23 from being worn due to the change in relative position of the inner liner 2 with the feed pipe 20 during rotation.

[0036] In a further preferred embodiment of the present invention, a guide rod 31 is fixedly installed on the top of the shell 1, a connecting plate 32 is slidably sleeved on the guide rod 31, the connecting plate 32 is fixedly connected to the first threaded rod 7, and a hanging ring 33 is fixedly installed on the top cover of the shell 1, and the hanging ring 33 is used to hang the top cover.

[0037] In this embodiment, when the threaded barrel 6 rotates, the first threaded rod 7 will slide up and down along the threaded barrel 6. Since the connecting plate 32 is fixed to the first threaded rod 7 and is slidably sleeved on the guide rod 31, when the first threaded rod 7 slides up and down, the connecting plate 32 will slide accordingly along the guide rod 31. The guide rod 31 provides a guiding function for the up and down movement of the first threaded rod 7, ensuring that the first threaded rod 7 can move stably and accurately along the predetermined direction. In addition, a hanging ring 33 is fixedly installed on the top cover of the shell 1. The function of the hanging ring 33 is to hoist the top cover. When it is necessary to open the top cover to operate the inner tank 2 (such as replacement, maintenance, etc.), it can be connected to the hanging ring 33 through a hoisting device to lift the top cover.

[0038] In order to further improve the use effect of this device, in addition to the above scheme, this scheme also has the following embodiments: In another embodiment of the present invention, a sleeve 28 is fixedly installed on the top of the inner tank 2, and the sleeve 28 is sleeved on the connecting rod 3. An annular scraper 29 for scraping materials on the connecting rod 3 is fixedly installed in the sleeve 28. A sealing ring 30 for sealing the gap between the connecting rod 3 and the sleeve 28 is also installed in the sleeve 28.

[0039] In this embodiment, when the connecting rod 3 moves up and down, the annular scraper 29 in the sleeve 28 will contact the surface of the connecting rod 3, thereby scraping off the material that may be attached to the connecting rod 3. At the same time, the sealing ring 30 installed in the sleeve 28 is used to seal the gap between the connecting rod 3 and the sleeve 28 to prevent the material from leaking from the gap to the outside of the sleeve 28.

[0040] In another embodiment of the present invention, the shell 1 is provided with multiple groups of peristaltic stirring mechanisms for peristaltic stirring of materials, and the peristaltic stirring mechanisms include: a connecting box 34 fixedly mounted on one side of the shell 1; a one-way screw 35 rotatably mounted in the connecting box 34; a connecting block 36 threadedly sleeved on the one-way screw 35, and the connecting block 36 is in sliding contact with the inner wall of the connecting box 34; a rectangular telescopic rod 37 fixedly mounted on one side of the connecting block 36, and a connecting frame 38 is mounted on the rectangular telescopic rod 37; a group of rollers 39 mounted on one side of the connecting frame 38 and arranged in an arc shape, and a group of the rollers 39 are in contact with the outside of the silicone shell 201; and a connecting mechanism provided on the shell 1 and the connecting block 36 for driving a group of rollers 39 to reciprocate left and right.

[0041] In this embodiment, when the one-way screw 35 rotates, the connecting block 36 will slide up and down along the one-way screw 35, driving the rectangular telescopic rod 37, the connecting frame 38 and the roller 39 to move up and down. At the same time, the connecting mechanism operates synchronously. Under the action of the connecting mechanism, a set of rollers 39 can also reciprocate left and right during the up and down movement. The rollers 39 will squeeze the silicone shell 201 when moving left and right. Due to the flexibility of the silicone shell 201, this squeezing will cause the inner liner 2 to peristalsis, thereby stirring the material in the inner liner 2. The peristaltic stirring mechanism uses the peristaltic effect generated by the roller 39 squeezing the inner liner 2 left and right, and cooperates with the up and down movement of the horizontal plate 4 and the rotation of the inner liner 2 itself to form a multi-mode stirring system. This composite stirring method can stir the material from different angles, so that the material is more evenly distributed in the reaction system, thereby enhancing the stirring effect.

[0042] In another embodiment of the present invention, the connecting mechanism includes: a mounting plate 40 fixedly mounted on one side of the connecting block 36; a toothless gear 41 rotatably mounted on the mounting plate 40; two third racks 42 both mounted in the connecting frame 38, and both of the third racks 42 are engaged with the toothless gear 41; a fourth rack 43 fixedly mounted in the housing 1; and a second gear 44 fixedly sleeved on the rotating shaft of the toothless gear 41, and the second gear 44 is engaged with the fourth rack 43.

[0043] In this embodiment, when the one-way screw drives the connecting block 36 to slide up and down, the connecting block 36 drives the mounting plate 40 installed on one side thereof to move up and down synchronously. Since the second gear 44 is fixedly sleeved on the rotating shaft of the toothless gear 41, and the second gear 44 is meshed with the fourth rack 43 fixedly installed in the housing 1, during the up and down movement of the mounting plate 40, the second gear 44 will rotate along the fourth rack 43, thereby driving the toothless gear 41 to rotate. Because the number of teeth of the toothless gear 41 and the third rack 42 are both six, when the toothless gear 41 is along the upper When the teeth of the third rack 42 on the left rotate, the meshing action of the teeth will push the connecting frame 38 and the roller 39 to move to the left; when the toothless gear 41 rotates to disengage from the third rack 42 located above and begins to rotate along the teeth of the third rack 42 located below, it will push the connecting frame 38 and the roller 39 to move to the right and reset. This cycle is repeated to achieve the left and right reciprocating motion of the roller 39. Through the meshing relationship between the toothless gear 41 and the two third racks 42 and the specific setting of the number of teeth, the left and right movement of the roller 39 can be accurately controlled.

[0044] In another embodiment of the present invention, an annular box 45 is fixedly provided on the shell 1, and the annular box 45 is fixedly connected to the top of the connecting box 34. The tops of multiple groups of one-way screws 35 are fixedly installed with a first sprocket 46. Multiple limiting wheels 47 are rotatably installed in the annular box 45. A first chain 48 is provided on the multiple first sprockets 46 and the limiting wheels 47, and the first chain 48 is engaged with the multiple limiting wheels 47 and the first sprocket 46.

[0045] In this embodiment, when the linkage mechanism drives one of the one-way screws 35 to rotate, the first sprocket 46 at the top of the one-way screw 35 will rotate accordingly. Since the first sprocket 46 is engaged with the first chain 48, the rotation of the first sprocket 46 will drive the first chain 48 to move. During the movement, the first chain 48 will drive other first sprockets 46 engaged with the first chain 48 to rotate, and the other first sprockets 46 are respectively fixedly mounted on the top of the corresponding one-way screw 35, thereby realizing the synchronous rotation of multiple groups of one-way screws 35. After the multiple groups of one-way screws 35 rotate synchronously, the connecting blocks 36 on each one-way screw 35 will slide up and down synchronously, and then drive the rollers 39 in each group of peristaltic stirring mechanisms through the connecting mechanism to achieve synchronous up and down movement and left and right reciprocating motion, ultimately making the multiple groups of peristaltic stirring mechanisms operate synchronously.

[0046] In another embodiment of the present invention, a linkage mechanism is provided on the threaded barrel 6 and the one-way screw 35 for synchronously driving the threaded barrel 6 and the one-way screw 35 to rotate, and the linkage mechanism includes: a first rotating rod 49 rotatably mounted on one side of the protective cover 5; a second rotating rod 50 rotatably mounted on the top of the shell 1; second sprockets 51 fixedly mounted on the first rotating rod 49 and the threaded barrel 6 respectively, and the two second sprockets 51 are provided with a second chain 52, and the second chain 52 is engaged with the two second sprockets 51; a round rod 53 rotatably provided on one side of the shell 1; two sets of bevel gears 54 fixedly mounted on one end of the first rotating rod 49 and the round rod 53 and at both ends of the second rotating rod 50, and the two sets of bevel gears 54 are engaged with each other; a rectangular seat 55 fixedly mounted on the output shaft of the second sprocket 51; a rectangular rod 56 mounted on the bottom end of the round rod 53, and the rectangular rod 56 is inserted into the rectangular seat 55.

[0047] In this embodiment, when the motor 8 drives the threaded barrel 6 to rotate, the second sprocket 51 rotates accordingly. Since the two second sprockets 51 are provided with a second chain 52 meshing with them, the second sprocket 51 on the threaded barrel 6 will drive the second sprocket 51 on the first rotating rod 49 to rotate through the second chain 52, thereby rotating the first rotating rod 49. The rotation of the first rotating rod 49 will drive the round rod 53 and the second rotating rod 50 to rotate through the meshing transmission of the bevel gear 54. The rotation of the round rod 53 will transmit the rotational force to the rectangular seat 55 through the cooperation of the rectangular rod 56 and the rectangular seat 55. The rectangular seat 55 drives the one-way screw 35 and the first sprocket 46 to rotate, thereby finally realizing the rotation of the one-way screw 35, and achieving the linkage function of synchronous rotation of the threaded barrel 6 and the one-way screw 35.

[0048] In another embodiment of the present invention, the bottom of the shell 1 is provided with a detection mechanism for detecting whether the inner tank 2 is leaking, and the detection mechanism includes: a collection box 57 fixedly installed on the bottom of the shell 1; a bracket 58 fixedly installed on the shell 1; a connecting rod 59 slidably installed on the bracket 58, and the bottom end of the connecting rod 59 is fixedly installed with a sealing block 60, and the sealing block 60 is in contact with the inner wall of the collection box 57; a spring 61 is sleeved on the connecting rod 59, and the two ends of the spring 61 are respectively fixedly connected to the sealing block 60 and the bracket 58, and a support plate 62 is fixedly installed on one side of the inner wall of the collection box 57, and the support plate 62 is located below the sealing block 60, and a switch device 63 is installed on the support plate 62.

[0049] In this embodiment, when the silicone shell 201 is damaged and the material in the inner liner 2 overflows, the material will flow into the collection box 57. As the material continues to accumulate in the collection box 57 and the weight increases, the sealing block 60 will be under downward pressure and move downward. At the same time, the spring 61 will be stretched. When the sealing block 60 drops to contact the switch device 63, the switch device 63 will be triggered and send a signal to the control system, thereby alerting the workers that the inner liner 2 is leaking.

[0050] In another embodiment of the present invention, an elastic steel plate 64 is installed on one side of the inner wall of the collection box 57, and the elastic steel plate 64 is adapted to the card slot of the sealing block 60. A third threaded rod 65 is rotatably installed on the bottom of the collection box 57, and a rectangular tube 66 is threadedly sleeved on the third threaded rod 65, and the rectangular tube 66 is in contact with one side of the support plate 62.

[0051] In this embodiment, when the inner liner 2 leaks, the material flows into the collection box 57 and accumulates on the sealing block 60, causing the sealing block 60 to move downward and contact the switch device 63. The elastic steel plate 64 will deform and get stuck in the slot of the sealing block 60, thereby limiting the sealing block 60 and preventing the sealing block 60 from moving at will due to the elastic force of the spring 61 or other factors, ensuring that the switch device 63 is continuously in a triggered state, and stably transmitting signals to the control system to alert workers that the inner liner 2 is leaking.

[0052] When it is necessary to reset the sealing block 60, the third threaded rod 65 rotatably installed at the bottom of the collecting box 57 is rotated. Since the rectangular tube 66 is threadedly sleeved on the third threaded rod 65, and the rectangular tube 66 is in contact with one side of the support plate 62, under the limiting action of the support plate 62, rotating the third threaded rod 65 will cause the rectangular tube 66 to move upward along one side of the support plate 62. As the rectangular tube 66 rises, it will push the sealing block 60 out of the elastic steel plate 64, releasing the limiting position of the elastic steel plate 64 on the sealing block 60. Then, under the elastic force of the spring 61, the sealing block 60 will return to its initial position, completing the reset operation.

[0053] The present invention also provides an enzymatic hydrolysis method for an enzymatic hydrolysis device used in a Paris polyphylla saponin extraction process, comprising the following steps: Step 1: Add the raw material of Paris polyphylla saponin into the feed pipe 20 through the bellows 21, and the raw material enters the inner pot 2 through the bottom of the feed pipe 20. After all the materials are poured in, start the motor 8. The output shaft of the motor 8 drives the differential wheel 9 to rotate, and the differential wheel 9 on the threaded barrel 6 is driven by the belt 10 to rotate, thereby driving the threaded barrel 6 to rotate. The first threaded rod 7 moves up and down under the rotation of the threaded barrel 6, driving the connecting rod 3 to move up and down in the inner pot 2, and the horizontal plate 4 moves up and down in a small range accordingly, moving the lower layer of raw materials upward to achieve preliminary stirring. The motor While the machine is running, the one-way screw 35 is driven to rotate by the linkage mechanism, and the connecting block 36 slides up and down under the rotation of the one-way screw 35, driving the rectangular telescopic rod 37, the connecting frame 38 and the roller 39 to move up and down. The connecting mechanism causes the roller 39 to reciprocate left and right during the up and down movement. The roller 39 squeezes the silica gel shell 201, causing the inner liner 2 to produce peristalsis, further stirring the material. The peristaltic stirring is combined with the horizontal plate stirring to avoid the damage of the enzyme molecular structure by mechanical shear force, maintain the enzyme activity, and improve the efficiency of the enzymatic reaction. Step 2: The hydraulic cylinder 18 drives the first rack 19 to move, driving the driving gear 17 to rotate, and then drives the driven gear 16 to rotate. The driven gear 16 engages with the ring gear 15, driving the inner container 2 to rotate. The rotation angle of the inner container 2 is controlled at 20 to 40 degrees, further changing the position and distribution of the materials to make the mixing more uniform. Step 3: As the first rack 19 moves, the second rack 27 drives the first gear 26 to rotate, thereby driving the second threaded rod 24 to rotate. The slider 25 moves upward under the rotation of the second threaded rod 24, driving the feed pipe 20 to move upward, so that a safe distance is maintained between the feed pipe 20 and the top of the inner container 2, to prevent the inner container 2 from colliding with the feed pipe 20 or wearing the annular sealing gasket 23 when it rotates later; Step 4: Hot water enters the jacket 13 through the pipe. The jacket 13 made of high thermal conductivity stainless steel quickly transfers heat to the environment around the inner tank 2, causing the material in the inner tank 2 to heat up. The temperature sensors 14 in the shell 1 and the inner tank 2 monitor the temperature in real time. The operator adjusts the hot water flow rate, temperature and other parameters according to the temperature information to ensure that the enzymatic hydrolysis reaction proceeds within the appropriate temperature range. Step 5: After the enzymatic hydrolysis reaction is completed, the material is discharged from the discharge pipe. When the inner liner 2 needs to be replaced, since the inner liner 2 is firmly fixed in the shell 1 by the support balls 12 and the support wheels 68 and is not fixedly connected to the shell 1, the inner liner 2 can be lifted out of the shell 1 by lifting equipment for replacement. After replacing the new inner liner 2, the feeding, enzymatic hydrolysis and stirring operations are repeated according to the above steps.

[0054] Step 6: When the silicone shell 201 is damaged and the material overflows and flows into the collection box 57, the material accumulates and causes the sealing block 60 to move downward, contacting the switch device 63, triggering a signal to alert the worker.

[0055] In summary, compared with related technologies, this device avoids the destruction of enzyme activity by mechanical shear force and improves the enzymatic reaction efficiency and saponin extraction rate by adopting a multi-mode stirring method that combines up and down movement of the horizontal plate, peristaltic stirring and rotation of the inner tank.

[0056] In the several embodiments provided in this application, it should be understood that the disclosed device can be implemented in other ways.

[0057] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the scope of protection of the invention. Obviously, the embodiments described are only some embodiments of the present invention, rather than all embodiments. Based on these embodiments, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in this field can still combine, add, delete or make other adjustments to the features in the various embodiments of the present invention according to the circumstances without conflict, without making creative work, so as to obtain different other technical solutions that do not deviate from the concept of the present invention in essence, and these technical solutions also fall within the scope of protection of the present invention.

Claims

1. An enzymatic hydrolysis device for extracting Paris polyphylla saponins, characterized in that: include: A shell and an inner container provided in the shell for enzymatically decomposing a raw material of Paris polyphylla saponin; A connecting rod is slidably arranged in the inner container, and a horizontal plate for stirring the raw materials in an up and down reciprocating manner is fixedly installed at the bottom end of the connecting rod; a protective cover fixedly mounted on the top of the housing; Rotating a threaded barrel mounted on the top of the protective cover; a first threaded rod threadably sleeved on the threaded barrel and detachably connected to the connecting rod, the first threaded rod being used to drive the connecting rod to move up and down; A driving mechanism is provided on the protective cover and is used to drive the threaded barrel to rotate.

2. The enzymatic hydrolysis device for extracting parsnip saponins according to claim 1, wherein The driving mechanism comprises: A motor fixedly mounted on one side of the protective cover; Differential wheels are fixedly mounted on the motor output shaft and the threaded cylinder respectively; A belt mounted on the differential wheel.

3. The enzymatic hydrolysis device for extracting parsnip saponins according to claim 1, wherein: The inner liner consists of a silicone shell, a top plate, a bottom plate and an annular sleeve. The top plate is installed on the top of the silicone shell, the bottom plate is arranged on the silicone shell, the annular sleeve is rotatably installed on the bottom plate, and the annular sleeve is fixedly connected to the silicone shell.

4. The enzymatic hydrolysis device for extracting parsnip saponins according to claim 3, wherein: A discharge pipe is installed at the bottom of the base plate, and a conical plate is fixedly sleeved on the discharge pipe, and the conical plate is adapted to the conical notch at the bottom of the shell. A support ball is fixedly installed at the bottom of the inner wall of the shell, and the support ball is in contact with the base plate. A support block is fixedly installed on one side of the inner wall of the shell, and a support wheel is fixedly installed on the top of the support block, and the support wheel is in close contact with the bottom of the top plate.

5. The enzymatic hydrolysis device for extracting Paris polyphylla saponins according to claim 1, wherein: A jacket is installed in the shell, and the material of the jacket is high thermal conductivity stainless steel. Two temperature sensors for monitoring the temperature of the shell and the inner tank are fixedly installed in the shell and the inner tank.

6. The enzymatic hydrolysis device for extracting Paris polyphylla saponins according to claim 1, wherein: The housing is provided with a rotation mechanism for driving the inner container to rotate, and the rotation mechanism includes: A gear ring fixedly mounted on the top of the inner tank; Rotating a driven gear mounted on the top of the inner wall of the housing, wherein the driven gear is engaged with the ring gear; A driving gear fixedly sleeved on the driven gear shaft; A hydraulic cylinder is installed on the top of the inner wall of the housing, and a first rack is fixedly installed on the hydraulic cylinder, and the first rack is meshed with the driving gear.

7. The enzymatic hydrolysis device for extracting Paris polyphylla saponins according to claim 6, wherein: A feed pipe is slidably installed on the top of the shell, a bellows is installed on the top of the feed pipe, annular pressure plates are fixedly installed on the top of the inner tank and the bottom of the feed pipe, and annular sealing gaskets are installed on both of the annular pressure plates, and the two annular sealing gaskets are in close contact.

8. The enzymatic hydrolysis device for extracting Paris polyphylla saponins according to claim 7, wherein: A height adjustment mechanism is provided in the housing for adjusting the height of the feed pipe, and the height adjustment mechanism includes: a second threaded rod rotatably mounted on the top of the inner wall of the housing; a slider threadedly sleeved on the second threaded rod, the slider being fixedly connected to an annular pressure plate located on the feed pipe; a first gear fixedly sleeved on the second threaded rod; A second rack is installed on one side of the first rack, and the second rack is meshed with the first gear.

9. The enzymatic hydrolysis device for extracting Paris polyphylla saponins according to claim 1, wherein: A guide rod is fixedly installed on the top of the shell, a connecting plate is slidably sleeved on the guide rod, and the connecting plate is fixedly connected to the first threaded rod. A hanging ring is fixedly installed on the top cover of the shell, and the hanging ring is used for hanging the top cover.

10. The enzymatic hydrolysis method of the enzymatic hydrolysis device for extracting Paris polyphylla saponins according to any one of claims 1 to 9, characterized in that: The steps include: Step 1: Add the Paris polyphylla saponin raw material into the feed pipe through the bellows, and the raw material enters the inner container through the bottom of the feed pipe. After all the materials are poured in, start the motor, and the motor output shaft drives the differential wheel to rotate. Through the belt transmission, the differential wheel on the threaded barrel rotates, and then drives the threaded barrel to rotate. The first threaded rod moves up and down under the rotation of the threaded barrel, driving the connecting rod to move up and down in the inner container, and the horizontal plate moves up and down in a small range accordingly, moving the lower layer of raw materials upward to achieve preliminary stirring; Step 2: The hydraulic cylinder drives the first rack to move, driving the driving gear to rotate, and then drives the driven gear to rotate. The driven gear meshes with the ring gear, driving the inner pot to rotate. The rotation angle of the inner pot is controlled at 20 to 40 degrees, further changing the position and distribution of the material to make the mixing more uniform. Step 3: As the first rack moves, the second rack drives the first gear to rotate, which in turn drives the second threaded rod to rotate. The slider moves upward under the rotation of the second threaded rod, driving the feed pipe upward to maintain a safe distance between the feed pipe and the top of the inner container, preventing the inner container from colliding with the feed pipe or wearing the annular sealing gasket during subsequent rotation; Step 4: Hot water enters the jacket through the pipe. The high thermal conductivity stainless steel jacket quickly transfers heat to the surrounding environment of the inner tank, causing the material in the inner tank to heat up. Temperature sensors in the shell and inner tank monitor the temperature in real time. The operator adjusts the hot water flow rate, temperature and other parameters based on the temperature information to ensure that the enzymatic hydrolysis reaction proceeds within the appropriate temperature range. Step 5: After the enzymatic hydrolysis reaction is completed, the material is discharged from the discharge pipe. When the inner liner needs to be replaced, since the inner liner is firmly fixed in the shell by the support balls and support wheels and is not fixedly connected to the shell, the inner liner can be lifted out of the shell for replacement by lifting equipment. After replacing the new inner liner, follow the above steps to repeat the feeding, enzymatic hydrolysis and stirring operations.

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