Application and method of sorghum aquaporin PIP1-5 gene in cultivation of transgenic plant

Abstract

The invention belongs to the field of crop genetic breeding, and relates to an application and a method of a sorghum aquaporin PIP1-5 gene in cultivation of a transgenic plant, the aquaporin gene is cloned from a drought-enduring crop sorghum, the aquaporin gene is named as PIP1-5, and transgenic yeast and transgenic arabidopsis experiments prove that the gene plays a role in drought tolerance. In addition, drought stress experiments and physiological index detection prove that the oilseed rape transfected with the gene has good drought tolerance.

Description

Technical Field

[0001] The invention belongs to the field of crop genetic breeding and relates to the cultivation of drought-resistant rapeseed. Background Art

[0002] Sorghum (Sorghum bicolor L.) is one of the world's major food crops in arid regions, boasting strong drought tolerance. It is widely cultivated in arid regions such as northern, northwestern, and southwestern my country. Rapeseed (Brassica napus L.), one of the world's four major oilseed crops, is also a major source of edible vegetable oil in my country. The Yangtze River Basin is the primary producer of winter rapeseed in my country. Many provinces in this basin are prone to autumn and winter droughts during the autumn and winter seasons, which severely impact rapeseed emergence and seedling growth. Northwest my country is the primary producer of spring rapeseed and serves as a crucial seed production base for winter rapeseed in southern China. Currently, Northwest China, including Gansu and Qinghai, is the primary summer planting area for winter rapeseed in my country. This arid and semi-arid climate offers ample sunlight and high rapeseed yields. Annual rapeseed seed production covers over 100,000 mu (approximately 16,000 mu) of land, producing over 2 million kilograms of seed. However, seasonal droughts are common during the rapeseed growing season in this region, severely impacting its normal growth. Extreme drought conditions can even lead to plant death. The Northwest region suffers from drought and water shortages. Irrigation water prioritizes grain crops like wheat, corn, and potatoes, followed by crops like rapeseed. Consequently, rapeseed production in the region suffers significant yield losses in drought years. Cultivating drought-tolerant rapeseed varieties can effectively improve rapeseed's adaptability to drought conditions and ensure its production.

[0003] Aquaporins (AQPs) are transmembrane proteins that selectively and efficiently transport water molecules on the cell membrane. They belong to the Major Intrinsic Protein (MIP) superfamily of intrinsic proteins and have highly conserved structural characteristics. AQPs control the transmembrane transport of water and substances. In plants, PIPs (intrinsic plasma membrane proteins) are the largest class of AQPs and are primarily responsible for the transport of substrates inside and outside the cell. PIPs are divided into two families, PIP1 and PIP2. The main structural difference between the two is that the PIP1 subfamily has a longer N-terminus and a shorter C-terminus than the PIP2 subfamily. Some PIP1 family aquaporins cannot function independently and must form tetramers with PIP2 family aquaporins to promote water transport. Most existing research aims to improve the growth performance and stress resistance of a species by overexpressing aquaporins from the same species. For example, application publication number CN107474126A overexpresses corn aquaporins in corn to obtain corn varieties with significantly improved yield and stress resistance. Application publication number CN101880674A overexpresses jujube aquaporins in jujube trees to obtain salt- and drought-tolerant varieties. Application publication number CN112321690A overexpresses soybean aquaporins in soybeans to obtain soybean varieties with improved drought resistance. However, can the transfer of aquaporins from other species into rapeseed produce the same effect? ​​The applicant has conducted in-depth research on this issue. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention proposes an application and method of a sorghum water channel protein PIP1-5 gene in cultivating transgenic plants.

[0005] The technical solution of the present invention is achieved as follows:

[0006] Application of a sorghum water channel protein PIP1-5 gene in cultivating transgenic plants, wherein the sorghum water channel protein PIP1-5 gene is shown in SEQ ID No. 1.

[0007] The application of the sorghum water channel protein PIP1-5 gene in cultivating transgenic plants specifically comprises the following steps: using transgenic technology to overexpress the sorghum water channel protein PIP1-5 gene in rapeseed plants to obtain transgenic plants.

[0008] A recombinant expression vector is an overexpression vector of a sorghum water channel protein PIP1-5 gene; the nucleotide sequence of the sorghum water channel protein PIP1-5 gene is shown in SEQ ID No.1.

[0009] The use of the sorghum aquaporin PIP1-5 gene or recombinant expression vector in cultivating transgenic plants, wherein the transgenic plants have at least one of the following conditions:

[0010] (1) Improved drought stress resistance;

[0011] (2) The germination rate of seeds under drought stress is improved;

[0012] (3) plant height increases under drought stress;

[0013] (4) The number of siliques per plant increased under drought stress.

[0014] The application of the above-mentioned recombinant expression vector in cultivating transgenic plants comprises the following steps: the recombinant expression vector is transformed into the improved plant by Agrobacterium transformation, thereby obtaining the transgenic plant.

[0015] A method for cultivating drought-resistant rapeseed comprises the following steps: constructing an overexpression vector of a sorghum water channel protein PIP1-5 gene, and then transferring the overexpression vector into a plant to be improved through a genetic transformation method.

[0016] The nucleotide sequence of the sorghum water channel protein PIP1-5 gene is shown in SEQ ID No. 1; the plant is Arabidopsis thaliana or rapeseed.

[0017] The present invention has the following beneficial effects:

[0018] 1. The present invention provides a gene, PIP1-5, that improves rapeseed drought tolerance. This gene comprises the DNA fragment shown in SEQ ID NO. 1. This gene was cloned from drought-tolerant wild sorghum in Inner Mongolia. It encodes an aquaporin, a transmembrane protein that efficiently transports water molecules across plant cell membranes. Under drought conditions, this gene promotes soil water uptake by plant root cells, thereby improving plant drought tolerance. The present invention aims to improve rapeseed drought tolerance through transgenic technology, addressing autumn and winter droughts in my country's main rapeseed-producing regions along the Yangtze River, as well as arid and semi-arid climates in Northwest China and Inner Mongolia, thereby promoting rapeseed production in my country.

[0019] 2. This invention exogenously expressed the PIP1-5 gene from wild-growing sorghum in yeast, Arabidopsis thaliana, and rapeseed. Drought tests showed that the drought tolerance of the transgenic yeast, Arabidopsis thaliana, and rapeseed plants was improved compared to their non-transgenic controls, demonstrating that expressing the PIP1-5 gene can enhance drought tolerance in recipients. Specifically, the drought-tolerant wild-growing sorghum aquaporin gene, PIP1-5, was expressed in rapeseed, resulting in the creation of a new drought-tolerant rapeseed variety. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0021] Figure 1 This is the base sequence of the PIP1-5 gene.

[0022] Figure 2 The amino acid sequence of the protein encoded by the PIP1-5 gene.

[0023] Figure 3 This is the subcellular localization of the sorghum water channel protein PIP1-5 gene; GFP: green fluorescent protein; RFP: red fluorescent protein; Bright: bright field image; Merge: overlay image.

[0024] Figure 4 For the positive identification of yeast transformed with pYES2-PIP; 1, 2, 3, 4, 5, and 6 are pYES2-PIP1-5 positive yeasts, 7 is the pYES2 empty vector, M: Marker.

[0025] Figure 5 Transgenic PIP1-5 yeast (INVSc1) exhibits drought tolerance.

[0026] Figure 6 For the positive identification of PIP1-5 gene-transgenic Arabidopsis plants; 1-5, 7, 9-16: positive Arabidopsis plants; 6, 8: false positive plants; WT: non-transgenic control plant; M: 1kb DNA marker.

[0027] Figure 7 For the positive identification of transgenic rapeseed with PIP1-5 genes; 1-13: positive rapeseed plants; WT: non-transgenic control plant; M: 1kb DNA marker.

[0028] Figure 8 This is the effect of drought stress on the germination of transgenic PIP1-5 gene Arabidopsis seeds; A1: 0% PEG6000; A2: 10% PEG6000; A3: 15% PEG6000; A4: 20% PEG6000.

[0029] Figure 9 The effect of drought stress on transgenic PIP1-5 Arabidopsis seedlings.

[0030] Figure 10The figure shows the effects of drought stress (20% PEG6000) on the physiological indicators of transgenic PIP1-5 Arabidopsis seedlings; 0%: no field PET6000 in the solid culture medium; 20%: 20% PEG6000 added to the solid culture medium.

[0031] Figure 11 The effect of drought stress on the germination of transgenic rapeseed seeds with PIP1-5 gene.

[0032] Figure 12 The effect of drought on transgenic rapeseed seedlings with PIP1-5 gene; a: phenotype of control WT and transgenic rapeseed with PIP1-5 gene before treatment; b: phenotype of control WT and transgenic rapeseed with PIP1-5 gene after 5 days of treatment.

[0033] Figure 13 The growth of transgenic rapeseed root system of PIP1-5 gene under different concentrations of PEG6000 treatment conditions; A1: 0% PEG; A2: 10% PEG; A3: 15% PEG; A4: 20% PEG; A5: 25% PEG; B: fresh weight of underground roots; C: dry weight of underground roots.

[0034] Figure 14 The growth conditions of transgenic PIP1-5 rapeseed under natural drought conditions; 1: Normally watered plants (same soil quality in each pot, same watering amount each time, maintaining soil relative humidity >80%); 2, 3, 4: Drought-treated plants (same soil quality in each pot, same watering amount each time, maintaining soil relative humidity less than 50%).

[0035] Figure 15 The effects of drought stress with different concentrations of PEG6000 on the physiology of transgenic rapeseed with PIP1-5 gene. DETAILED DESCRIPTION

[0036] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] Unless otherwise specified, the experimental methods used in the following experimental examples are conventional methods; the materials and reagents used are commercially available reagents and materials unless otherwise specified.

[0038] The invention relates to an application of a sorghum water channel protein PIP1-5 gene in cultivating transgenic plants. The sorghum water channel protein PIP1-5 gene has a similarity of more than 90% with the nucleotide sequence shown in SEQ ID No. 1, and preferably at least 95% of the nucleotide sequence.

[0039] Preferably, the sorghum water channel protein PIP1-5 gene is shown as SEQ ID No. 1.

[0040] The application of the sorghum water channel protein PIP1-5 gene in cultivating transgenic plants specifically comprises the following steps: using transgenic technology to overexpress the sorghum water channel protein PIP1-5 gene in rapeseed plants to obtain transgenic plants.

[0041] A recombinant expression vector is an overexpression vector of a sorghum water channel protein PIP1-5 gene; the nucleotide sequence of the sorghum water channel protein PIP1-5 gene is shown in SEQ ID No.1.

[0042] The use of the sorghum aquaporin PIP1-5 gene or recombinant expression vector in cultivating transgenic plants, wherein the transgenic plants have at least one of the following conditions:

[0043] (1) Improved drought stress resistance;

[0044] (2) The germination rate of seeds under drought stress is improved;

[0045] (3) plant height increases under drought stress;

[0046] (4) The number of siliques per plant increased under drought stress.

[0047] The following is an explanation based on specific experiments:

[0048] Example

[0049] 1 Cloning of PIP1-5 genes

[0050] Based on the sorghum aquaporin gene sequence in the NCBI database, forward primers and reverse primers were designed upstream and downstream of the gene, and a new sorghum aquaporin gene was amplified from wild sorghum in Inner Mongolia and named PIP1-5. The gene is 873 bp and the nucleotide sequence is shown in SEQ ID No. 1. Figure 1 , encoding a 290aa protein, the amino acid sequence of which is shown in SEQ ID No.2. Figure 2 .

[0051] 2 Subcellular localization of PIP1-5 gene products

[0052] The PIP1-5 genes were connected to the vector pCAMBIA1302 to construct the subcellular localization vector pC-PIP. pC-PIP and the empty vector pCAMBIA1302 were respectively transformed into the GV3101 strain, and positive Agrobacterium was selected and cultured at 28°C and 200 rpm until the OD 600 The value was 0.8, and after being suspended in suspension, it was co-injected with the plasma membrane co-localization marker strain pm-rk into Nicotiana benthamiana leaves, and the protein localization was observed under a laser confocal microscope. The results showed that Figure 3 As shown, GFP signals were detected in both the nucleus and the cell membrane in the empty vector control group, while GFP signals were only detected on the cell membrane in the experimental group (pC-PIP). These results indicate that PIP1-5 gene products function on the cell membrane, consistent with the characteristics of aquaporins.

[0053] 3. Study on drought tolerance of yeast transformed with PIP1-5 genes

[0054] PIP1-5 was ligated with the yeast expression vector pYES2.0 to construct the yeast expression vector pYES2-PIP, which was then transformed into the yeast strain INVSc1. Single clones were selected from the SD-Ura plate for PCR positive identification. Figure 4 The electrophoresis shown shows that strains 1, 2, 3, 4, 5, and 6 contain the target genes PIP1-5.

[0055] The yeast cells transformed with PIP1-5 gene positive and the yeast cells transformed with empty vector (pYES2) were divided into the following groups: 600 The bacterial solution was diluted undiluted, 10-fold, 100-fold, and 1000-fold and then inoculated onto SG / -Ura solid medium containing 0 mol / L, 0.8 mol / L, and 1 mol / L mannitol. The phenotype was observed after three days. Figure 5 As shown in the figure, on plates without mannitol (0 mol / L), the colony sizes of yeast expressing the PIP1-5 gene and yeast expressing the PIP1-5 gene were essentially identical. However, on SG-U solid medium containing 0.8 and 1 mol / L mannitol, more PIP1-5-transfected yeast colonies were observed than those of yeast expressing the PIP1-5 gene at a 1000-fold dilution. This result suggests that expressing the PIP1-5 gene in yeast improves drought tolerance.

[0056] 4. Obtaining PIP1-5 Transgenic Arabidopsis and PIP1-5 Transgenic Rapeseed

[0057] The PIP1-5 genes were ligated with pCAMBIA1300 to construct the plant binary expression vector pC1300-PIP. pC1300-PIP was transformed into Agrobacterium GV3101 using the freeze-thaw method. The PIP1-5 genes were then introduced into Arabidopsis col-0 and Brassica napus Xiangyou 18 using the floral dip method and the hypocotyl transformation method.

[0058] The primers PIPF5'-ACACTACATGGCGTGATTTCAT-3' and PIP-R5'-TCCACTATCGGCGAGTACTTCT-3' were used to screen positive plants. The PCR reaction conditions were 95℃, 3 min; 95℃, 15 s, 58℃, 15 s, 72℃, 30 s, 35 cycles. According to the PCR results Figure 6 、 Figure 7 , and obtained transgenic PIP1-5 gene-positive Arabidopsis and rapeseed plants.

[0059] Drought tolerance test of 5-transformed PIP1-5 Arabidopsis

[0060] 5.1 Developmental experiment of PIP1-5 transgenic Arabidopsis under drought stress

[0061] Two PIP1-5 gene Arabidopsis thaliana materials OE1 and OE2, as well as non-transgenic control Arabidopsis thaliana seeds were sown on MS solid medium containing different PEG6000 concentrations for germination test, and the germination rate was calculated on the seventh day. Figure 8 As shown in Table 1:

[0062] Table 1 Germination rates of transgenic Arabidopsis and control seeds under different drought conditions

[0063] 0% PEG 10% PEG 15% PEG 20% PEG WT 100 98 76 56 OE1 100 100 100 100 OE2 100 100 98 100

[0064] There was no significant difference in germination rate or final germination percentage between control and PIP1-5 transgenic Arabidopsis seeds under no PEG6000 or 10% PEG6000 treatment. However, on MS solid medium containing 15% PEG6000, the germination and final germination percentages of the two transgenic Arabidopsis plants were 100% and 98%, respectively, significantly higher than the 76% germination percentage of the control plants. The difference in germination and final germination percentages was even more pronounced on MS solid medium containing 20% ​​PEG6000. Both transgenic Arabidopsis plants achieved 100% germination, while the germination percentage of the control plants was only 56%. The transgenic plants had significantly higher germination rates than the control plants and grew faster than the wild-type control plants.

[0065] The above results indicate that under drought stress conditions, exogenous expression of sorghum PIP1-5 gene can promote the germination of Arabidopsis seeds, thereby improving the drought tolerance of transgenic Arabidopsis.

[0066] 5.2 Effects of drought stress on the root system of transgenic PIP1-5 Arabidopsis seedlings

[0067] The control plants and transgenic Arabidopsis thaliana OE1 and OE2 seedlings that had grown normally for 5 days on MS solid medium were transferred to MS solid medium containing different PEG6000 concentrations for drought stress treatment, and their phenotypes under drought stress were observed.

[0068] Table 2 Root growth of transgenic Arabidopsis and control under drought stress

[0069]

[0070] The results are as follows Figure 9 As shown in Table 2, control and transgenic Arabidopsis plants showed similar growth trends on MS solid medium without PEG6000 treatment. On MS solid medium containing 15% PEG6000, the average root lengths of the two transgenic Arabidopsis plants were 3.08 cm and 3.18 cm, significantly longer than the average root length of 2.20 cm for the non-transgenic control plants. On MS solid medium containing 20% ​​PEG6000, the growth of both transgenic and non-transgenic control plants was inhibited by drought stress. However, the average root lengths of the transgenic plants, 2.13 cm and 2.26 cm, were still significantly longer than the average root length of 1.16 cm for the non-transgenic plants. These results indicate that Arabidopsis expressing the sorghum PIP1-5 gene develops a more developed root system under drought stress, thereby improving the drought tolerance of the transgenic Arabidopsis plants.

[0071] 5.3 Effects of drought stress on physiological parameters of transgenic Arabidopsis thaliana seedlings with PIP1-5 gene

[0072] To reveal the physiological and biochemical mechanisms of drought tolerance in transgenic PIP1-5 Arabidopsis, the activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were measured in transgenic plants OE1 and OE2, as well as in controls. In addition, physiological indicators related to drought tolerance, such as proline (Pro), hydrogen peroxide (H2O2), and malondialdehyde (MDA), were also measured.

[0073] The results are as follows Figure 10The results show that under drought stress conditions (0% PEG6000), there were no differences in CAT, POD, and SOD enzyme activities, Pro content, H2O2 content, or MDA content between the control plants and the transgenic materials OE1 and OE2. Under drought stress conditions containing 20% ​​PEG6000, the CAT, POD, and SOD enzyme activities of the transgenic plants OE1 and OE2 were significantly higher than those of the control plants. Furthermore, Pro content was also significantly higher in the transgenic materials OE1 and OE2 than in the control plants. However, the H2O2 and MDA content of the control plants were significantly higher than those of the transgenic materials OE1 and OE2.

[0074] CAT, POD, and SOD are involved in repairing cellular damage and can effectively counteract cellular damage caused by drought stress. Increased intracellular Pro can effectively reduce water potential, making cells less susceptible to dehydration under drought stress, thereby improving drought resistance. H2O2 and MDA are harmful to cells; excessive levels can damage cell membranes and organelles. Transgenic Arabidopsis thaliana expressing the PIP1-5 gene exhibited higher CAT, POD, and SOD enzyme activities, higher Pro content, and lower H2O2 and MDA levels, demonstrating enhanced drought resistance.

[0075] Drought tolerance test of 6 transgenic rapeseed with PIP1-5 genes

[0076] 6.1 Germination test of transgenic rapeseed with PIP1-5 under drought stress

[0077] Two transgenic rapeseed lines carrying the PIP1-5 gene, PIP#3 and PIP#9, were tested for germination rate under drought stress in a germination box. After 7 days, the germination rates under treatment with different concentrations of PEG6000 were counted.

[0078] Table 3 Germination rates of transgenic rapeseed and control seeds under different drought conditions

[0079]

[0080] The results showed that ( Figure 11(Table 3) Under no PEG6000 and 10% PEG6000 treatment conditions, there was no significant difference in the final germination rate between the control plants and the transgenic materials PIP#3 and PIP#9, all exceeding 99%. However, when the PEG6000 concentration was increased to 15%, the germination rate of both transgenic materials reached 100%, while the germination rate of the WT control was only 67.67%. At a PEG6000 concentration of 20%, the germination rate of both transgenic materials reached over 99%, while the final germination rate of the WT control was only 60.00%. These results indicate that expression of the sorghum PIP1-5 gene in rapeseed can improve the germination rate of rapeseed seeds under drought stress.

[0081] 6.2 Effects of drought stress on transgenic rapeseed seedlings and roots of PIP1-5 transgenic rapeseed

[0082] Two transgenic rapeseed lines (PIP#9 and PIP#3) harboring the PIP1-5 gene, along with a control WT plant, were planted in pots. The plants were watered normally (soil moisture >80%) until they developed 3-4 leaves. Rapeseed seedlings of similar growth potential were then subjected to simulated drought treatment with varying concentrations of PEG6000 for 5 days. No watering was applied during the treatment period, and watering resumed after 5 days.

[0083] The results are as follows Figure 12 As shown, five days of drought affected all rapeseed treatments, with noticeable yellowing of leaves. One day after watering resumed, both transgenic and control plants recovered under 0%, 10%, and 15% PEG6000 treatments, with minimal phenotypic differences. Under 20% and 25% PEG6000 treatments, two transgenic accessions, PIP#9 and PIP#3, essentially resumed normal growth after watering resumed, while the control accession remained largely unchanged and remained near death.

[0084] Table 3 Root parameters of rapeseed under different concentrations of PET6000 treatment

[0085]

[0086] The roots of the rapeseeds with the above different treatments were taken out from the nutrient pots, cleaned, and the root length, fresh weight and dry weight were measured. Figure 13 As can be seen from Table 4, there were no significant differences in root length, dry weight and fresh weight between the control plants and the transgenic materials PIP#9 and PIP#3 under the treatments of 0% (A1) and 10% (A2) PEG6000.

[0087] Drought had a significant impact on rapeseed root growth as PEG6000 concentrations increased. At PEG6000 concentrations of 15%, 20%, and 25%, the root lengths of transgenic PIP#9 and PIP#3 plants were significantly longer than those of non-transgenic controls, and their dry and fresh weights were also significantly greater than those of the non-transgenic controls.

[0088] Two transgenic lines, PIP#3 and PIP#9, and a non-transgenic control were planted in pots in an artificial climate chamber. Each pot contained the same soil mass and was watered at the same rate. Before bud formation, the rapeseed plants were watered regularly, and the relative humidity (RH) was measured using a soil moisture meter to maintain it at >80%. After bud formation, the plants were watered less frequently to maintain RH <50%. A control planted with normal hydration (RH >80%) served as a control.

[0089] from Figure 14 The non-transgenic control (WT) is on the left, and the transgenic lines PIP#9 and PIP#3 are in the middle and on the right. As can be seen from the figure, the non-transgenic control (WT) and drought-treated plants (2, 3, and 4) are weak and produce few fruits, with plant 4, in particular, nearing death. However, the drought-treated transgenic PIP#9 and PIP#3 plants (2, 3, and 4) have higher plant height and more siliques per plant than the WT drought-treated plants, demonstrating better drought tolerance.

[0090] Increased soluble sugar content and SOD activity in plant cells are beneficial to drought tolerance, while increased H2O2 and MDA concentrations damage cells and are detrimental to plant drought tolerance. The SOD activity, H2O2, MDA, and soluble sugar content of two transgenic rapeseed lines (PIP#9 and PIP#3) expressing PIP1-5 and a non-transgenic control (WT) were measured.

[0091] Figure 15 The results showed that under drought treatment with 10%, 15%, 20%, and 25% PEG6000, H₂O₂ and MDA levels in transgenic plants were significantly lower than those in non-transgenic plants. However, soluble sugar content and SOD enzyme activity were significantly higher in transgenic plants than in non-transgenic plants. These results suggest that transgenic rapeseed plants with the PIP1-5 gene possess enhanced drought tolerance.

[0092] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. Application of the sorghum water channel protein PIP1-5 gene in cultivating transgenic plants, characterized by: The sorghum water channel protein PIP1-5 gene is shown in SEQ ID No.

1.

2. The use of the sorghum water channel protein PIP1-5 gene in cultivating transgenic rapeseed according to claim 1, characterized in that: Transgenic technology was used to overexpress the sorghum water channel protein PIP1-5 gene in rapeseed plants to obtain transgenic plants.

3. The use of the sorghum water channel protein PIP1-5 gene in cultivating transgenic rapeseed according to claim 2, characterized in that: The plant is Arabidopsis thaliana or rapeseed.

4. The use of the sorghum water channel protein PIP1-5 gene in cultivating transgenic plants according to claim 3, characterized in that: The transgenic plant has at least one of the following conditions: (1) Improved resistance to drought stress; (2) The germination rate of seeds under drought stress is improved; (3) Plant height increases under drought stress; (4) The number of siliques per plant increased under drought stress.

5. A recombinant expression vector, characterized in that: The recombinant expression vector is an overexpression vector of the sorghum water channel protein PIP1-5 gene; the nucleotide sequence of the sorghum water channel protein PIP1-5 gene is shown in SEQ ID No.

1.

6. Use of the recombinant expression vector according to claim 5 in cultivating transgenic plants, characterized in that: The transgenic plant has at least one of the following conditions: (1) Improved resistance to drought stress; (2) The germination rate of seeds under drought stress is improved; (3) Plant height increases under drought stress; (4) The number of siliques per plant increased under drought stress.

7. Use of the recombinant expression vector according to claim 5 in cultivating transgenic plants, characterized in that: The steps are: the recombinant expression vector is transformed into the plant to be improved through the Agrobacterium transformation method to obtain a transgenic plant; the plant is Arabidopsis thaliana or rapeseed.

8. A method for cultivating drought-resistant rapeseed, characterized in that: The method comprises the following steps: constructing an overexpression vector of a sorghum water channel protein PIP1-5 gene, and then transferring the overexpression vector into a plant to be improved through a genetic transformation method.

9. The method for cultivating drought-resistant rapeseed according to claim 8, characterized in that: The nucleotide sequence of the sorghum water channel protein PIP1-5 gene is shown in SEQ ID No.

1.

10. The method for cultivating drought-resistant rapeseed according to claim 9, characterized in that: The plant is Arabidopsis thaliana or rapeseed.

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