Preparation method and application of dendrobium candidum extract

Abstract

The present application relates to a preparation method and application of Dendrobium candidum extract, and belongs to the technical field of plant extraction. The present application realizes physical disruption of cell structure by using ultra-low temperature crushing technology, and avoids degradation of heat-sensitive components. A complex system of cellulase, pectinase and neutral protease is used for synergistic enzymolysis at 35-45 DEG C and pH 5-6, which efficiently hydrolyzes cell wall components and releases bound anti-inflammatory active ingredients. Subsequently, Ruminococcus lactaris is used for anaerobic fermentation, and the beta-glucosidase secreted by the bacteria hydrolyzes the bibenzyl aglycone, the metabolic product lactic acid promotes the protonation of alkaloids to form water-soluble salts, and the exopolysaccharide forms a complex active ingredient with the dendrobium polysaccharide; the fermentation broth is filtered, and is compounded with the ultrafiltration purified water extract obtained by ultrasonic assisted extraction at a ratio of (2-4):1, and finally high-purity extract is obtained by freeze-drying. The extract can be released controllably in the gastrointestinal tract, and the inhibition rate of LPS-induced inflammation is better than that of traditional processes, and can be used in the field of intestinal anti-inflammatory.

Description

Technical Field

[0001] This invention belongs to the field of plant extraction technology, and relates to a method for preparing and applying Dendrobium officinale extract. Background Technology

[0002] Dendrobium officinale is a traditional and precious Chinese medicinal herb, rich in polysaccharides, bibenzyl compounds, alkaloids, amino acids and other active ingredients. It has a variety of pharmacological effects such as nourishing yin and clearing heat, benefiting the stomach and promoting the production of body fluids, and enhancing immunity. Current research shows that it has antioxidant, anti-inflammatory and immunomodulatory activities.

[0003] Existing plant extraction methods have certain limitations. Conventional methods such as water extraction and alcohol extraction involve high temperatures and long extraction times, which may lead to the degradation or inactivation of heat-sensitive active ingredients, affecting the bioactivity of the final extract. Traditional methods usually pursue the yield of total polysaccharides or total extracts, but do not sufficiently enrich active components with specific bioactivities, resulting in unclear active ingredients and unstable effects in the product. In addition, components such as Dendrobium officinale polysaccharides may be degraded or incompletely absorbed in the upper digestive tract after oral administration, making it difficult to effectively reach and act on intestinal inflammation sites.

[0004] Therefore, there is an urgent need to develop a preparation method and application of Dendrobium officinale extract. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the present invention provides a method for preparing Dendrobium officinale extract and its application.

[0006] The objective of this invention can be achieved through the following technical solutions:

[0007] A method for preparing Dendrobium officinale extract, the specific steps of which are as follows:

[0008] S1: Fresh Dendrobium officinale strips were processed using ultra-low temperature pulverization technology. The pulverization temperature was -80~-90 ℃, and the pulverization time was 60~90 s. The ultra-low temperature pulverization was carried out under the protection of high-purity nitrogen with a nitrogen flow rate of 0.5~1 L / min to obtain material A.

[0009] S2: Place material A in PBS buffer to make the material-to-liquid ratio 1:(10~20), and enzymatically hydrolyze material A at 35~45 ℃. Add 5~10% of the mass of material A with a compound enzyme preparation, and enzymatically hydrolyze at 150~250 rpm for 4~6 h. Centrifuge to separate the enzymatic hydrolysate B and the enzymatic hydrolysis residue.

[0010] S3: Adjust the pH of the enzymatic hydrolysate B to 6.2-6.5, sterilize at 115℃ for 15 min, cool to room temperature, inoculate with 5% activated Lactobacillus rhamnosus culture, anaerobic culture at 37℃ for 18-24 h, then centrifuge at 4℃, and filter through a 0.22μm filter membrane to obtain fermentation supernatant C;

[0011] S4: Extract the enzymatic hydrolysis residue with pure water at an extraction temperature of 50-60 ℃, a material-to-liquid ratio of 1:(6-8), and an extraction time of 2-3 h. Simultaneously, turn on ultrasonic assistance. After sedimentation and centrifugation, the extract is filtered through a combination of 10μm and 2μm fine filtration membranes. The fine filtrate is then filtered through an ultrafiltration membrane (100 kDa) at an operating pressure of 0.1 MPa and a temperature of 25-30 ℃ to obtain aqueous extract D.

[0012] S5: Mix the fermentation supernatant C and the water extract D, pre-freeze at -40 ℃ for 4 h, and then freeze-dry for 20~24 h to obtain the Dendrobium officinale extract.

[0013] As a preferred embodiment of the present invention, the particle size of Dendrobium officinale after being treated with ultra-low temperature pulverization technology in S1 is D50≤50 μm.

[0014] As a preferred embodiment of the present invention, the complex enzyme preparation in S2 is cellulase, pectinase and neutral protease in a mass ratio of (1~3):1:1.

[0015] As a preferred embodiment of the present invention, the pH of the PBS buffer in S2 is 5-6.

[0016] As a preferred embodiment of the present invention, the viable count of *Lactobacillus rhamnosus* activated bacterial solution in S3 is ≥10. 9 CFU / mL.

[0017] As a preferred embodiment of the present invention, the centrifugation speed in step S3 is 8000~9000 rpm and the centrifugation time is 10~20 min.

[0018] In a preferred embodiment of the present invention, the ultrasonic frequency in step S4 is 10~20 kHz, and the power density is 0.3~0.5 W / cm². 2 .

[0019] As a preferred embodiment of the present invention, in step S5, the fermentation supernatant C and the water extract D are mixed at a volume ratio of (2~4):1.

[0020] An application of Dendrobium officinale extract, wherein the Dendrobium officinale extract can be used in the field of intestinal anti-inflammatory.

[0021] This invention first involves pulverizing Dendrobium officinale at -80 to -90°C, then rapidly freezing the intracellular water with liquid nitrogen to form micron-sized ice crystals. The volume expansion effect of these ice crystals mechanically disrupts the cell walls and membranes, achieving physical dissociation of the cell structure. This process avoids the breakage of polysaccharide β-glycosidic bonds and oxidation of alkaloid nitrogen heterocyclic structures caused by frictional heat generation in traditional mechanical pulverization. Nitrogen protection effectively inhibits the enzymatic oxidation of phenolic components and non-enzymatic browning reactions. After pulverization to a D50 ≤ 50 μm, the specific surface area of ​​the material is significantly increased, thereby significantly increasing the probability of substrate-enzyme contact during enzymatic hydrolysis.

[0022] A complex enzyme system was constructed using cellulase, pectinase, and neutral protease in a specific mass ratio. Cellulase efficiently hydrolyzes the β-1,4-glycosidic bonds of cell wall cellulose, disrupting the crystalline structure. Pectinase breaks down the α-1,4-galacturonic acid bonds of pectin in the intercellular matrix, reducing cell adhesion. Neutral protease specifically degrades glycoproteins covalently bound to polysaccharides, releasing bound polysaccharides and reducing the viscosity of the extract, thereby exposing or releasing the target anti-inflammatory active ingredients. Under conditions of 35–45℃ and pH 5–6, the complex enzymatic hydrolysis efficiency was significantly improved compared to the single-enzyme system, and the release of target components such as bibenzyl groups and alkaloids was significantly increased, while avoiding enzyme inactivation caused by high temperatures and the decomposition of heat-sensitive components of dendrobine.

[0023] After enzymatic hydrolysis, *Lactobacillus rhamnosus* was inoculated for anaerobic fermentation. The β-glucosidase produced by the bacteria specifically hydrolyzes *Dendrobium nobile* bibenzyl aglycones, releasing free anti-inflammatory active ingredients. The metabolite lactic acid lowers the system pH to 4.5-5.0, promoting the protonation of alkaloids and the formation of water-soluble salts, significantly improving extraction efficiency compared to ethanol extraction. The extracellular polysaccharides produced during fermentation form complexes with *Dendrobium nobile* polysaccharides, protecting the active ingredients from oxidation through steric hindrance. Filtration using a 0.22 μm membrane completely removes bacterial cells and large molecular impurities. Lactic acid in the fermentation broth forms lactate ester complexes with bibenzyl compounds, which decompose after freeze-drying, releasing high-purity active ingredients.

[0024] Ultrasonic-assisted water extraction breaks down incompletely enzymatically hydrolyzed cell debris, releasing arabinogalactan-protein complexes bound to the cell wall. A 10μm membrane initially traps larger particles, such as plant fragments and incompletely broken cell clusters, while a 2μm membrane further removes finer suspended matter, such as micron-sized colloidal particles. This staged filtration process reduces the load on individual membranes, extends their lifespan, lowers the risk of contamination in subsequent ultrafiltration steps, and improves product clarity. Ultrafiltration further enhances polysaccharide purity, and residual lactic acid in the water extract promotes polysaccharide protonation, strengthening electrostatic interactions with bibenzyl compounds.

[0025] When fermentation supernatant and water extract are mixed at a certain volume ratio, lactic acid and Dendrobium polysaccharides can form pH-sensitive nanogels in a simulated gastrointestinal tract, allowing for the controlled release of active ingredients. Freeze-drying avoids the glass transition phenomenon present in liquid water by directly sublimating ice crystals, preventing the dehydration condensation of polysaccharide hydroxyl groups. Compared with hot air drying, the retention rate of active ingredients after drying reaches over 98%.

[0026] The beneficial effects of this invention are:

[0027] This invention employs ultra-low temperature liquid nitrogen pulverization technology (-80~-90℃) to physically disrupt cell structure using the ice crystal expansion effect, preventing the breakage of β-glycosidic bonds in polysaccharides and the oxidation of alkaloids. Nitrogen protection inhibits phenolic browning, significantly increasing the specific surface area and enhancing enzymatic hydrolysis contact efficiency. A composite enzyme system synergistically hydrolyzes cell walls, intercellular matrix, and binding proteins, improving hydrolysis efficiency at 35~45℃ and pH 5~6, increasing the release of heat-sensitive components with a retention rate >95%. β-glucosidase produced by *Lactobacillus rhamnosus* fermentation hydrolyzes bibenzyl aglycones, and the metabolite lactic acid promotes alkaloid protonation, resulting in high extraction efficiency. Simultaneously, an extracellular polysaccharide-*Dendrobium* polysaccharide complex is formed to protect the active ingredients. Ultrasonic-assisted water extraction combined with ultrafiltration further improves polysaccharide purity. A mixture of fermentation broth and water extract at a ratio of (2~4):1 allows for controlled release in the gastrointestinal tract. The preparation method of this invention achieves efficient release, targeted enrichment, and synergistic effects of anti-inflammatory active ingredients. The extract's inhibition rate against LPS-induced inflammation models is significantly higher than commercially available products. Detailed Implementation

[0028] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0029] In the embodiments and comparative examples of this invention:

[0030] Cellulase: Purchased from Shanghai Yuanye Biotechnology Co., Ltd., derived from Trichoderma reesei ATCC26921;

[0031] Pectinase: Purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd., derived from Rhizopus genus;

[0032] Neutral protease: purchased from Shanghai Yuanye Biotechnology Co., Ltd., derived from Bacillus subtilis;

[0033] Lactobacillus rhamnosus LR22: provided by Shanghai Jiaotong University Angli Co., Ltd., accession number CNCM I-4474;

[0034] 10μm and 2μm fine filtration membranes: purchased from Merck Group, Germany;

[0035] Ultrafiltration membrane: purchased from Shanghai Sai'ao Separation Technology Engineering Co., Ltd.

[0036] Example 1

[0037] A method for preparing Dendrobium officinale extract, the specific steps of which are as follows:

[0038] S1: Fresh Dendrobium officinale strips were processed using ultra-low temperature pulverization technology. The pulverization temperature was -85 ℃ and the pulverization time was 75s. The ultra-low temperature pulverization was carried out under the protection of high-purity nitrogen with a nitrogen flow rate of 0.8 L / min, resulting in material A with a particle size of D50≤50 μm.

[0039] S2: Place material A in PBS buffer at pH 5.5 to make the material-to-liquid ratio 1:15, and enzymatically hydrolyze material A at 40 °C. Add 8% by mass of a compound enzyme preparation, which is a mixture of cellulase, pectinase and neutral protease in a mass ratio of 2:1:1. Hydrolyze at 200 rpm for 5 h, and centrifuge to obtain hydrolysate B and hydrolysate residue.

[0040] S3: Adjust the pH of the enzymatic hydrolysate B to 6.3, sterilize at 115 ℃ for 15 min, cool to room temperature, and then inoculate with 5% activated Lactobacillus rhamnosus culture. The viable count of the activated Lactobacillus rhamnosus culture should be ≥10. 9 The concentration of CFU / mL was anaerobically cultured at 37℃ for 20 h, followed by centrifugation at 4℃ at 8500 rpm for 15 min, and then filtered through a 0.22 μm filter membrane to obtain fermentation supernatant C.

[0041] S4: Extract the enzymatic hydrolysis residue with pure water at a temperature of 55 ℃, a material-to-liquid ratio of 1:7, and an extraction time of 2.5 h. Simultaneously, use ultrasonic assistance at a frequency of 15 kHz and a power density of 0.4 W / cm³. 2 After sedimentation and centrifugation, the extract was filtered through a combination of 10μm and 2μm fine filtration membranes. The filtrate was then filtered through an ultrafiltration membrane (100 kDa) at an operating pressure of 0.1 MPa and a temperature of 28 °C to obtain aqueous extract D.

[0042] S5: The fermentation supernatant C and the water extract D were mixed at a volume ratio of 3:1, pre-frozen at -40 ℃ for 4 h, and then freeze-dried for 22 h to obtain the Dendrobium officinale extract.

[0043] An application of Dendrobium officinale extract, wherein the Dendrobium officinale extract can be used in the field of intestinal anti-inflammatory.

[0044] Example 2

[0045] A method for preparing Dendrobium officinale extract, the specific steps of which are as follows:

[0046] S1: Fresh Dendrobium officinale strips were processed using ultra-low temperature pulverization technology. The pulverization temperature was -80 ℃ and the pulverization time was 60 s. The ultra-low temperature pulverization was carried out under the protection of high-purity nitrogen with a nitrogen flow rate of 0.5 L / min, resulting in material A with a particle size of D50≤50 μm.

[0047] S2: Place material A in PBS buffer at pH 5 to make the material-to-liquid ratio 1:10, and enzymatically hydrolyze material A at 35 °C. Add 5% by mass of a compound enzyme preparation, which is a mixture of cellulase, pectinase and neutral protease in a mass ratio of 1:1:1. Hydrolyze at 150 rpm for 4 h, and centrifuge to obtain hydrolysate B and hydrolysate residue.

[0048] S3: Adjust the pH of the enzymatic hydrolysate B to 6.2, sterilize at 115 ℃ for 15 min, cool to room temperature, and then inoculate with 5% activated Lactobacillus rhamnosus culture. The viable count of the activated Lactobacillus rhamnosus culture should be ≥10. 9 The concentration of CFU / mL was anaerobically cultured at 37℃ for 18 h, followed by centrifugation at 4℃ at 8000 rpm for 10 min, and then filtered through a 0.22 μm filter membrane to obtain fermentation supernatant C.

[0049] S4: Extract the enzymatic hydrolysis residue with pure water at a temperature of 50 ℃, a material-to-liquid ratio of 1:6, and an extraction time of 2 h. Simultaneously, use ultrasonic assistance at a frequency of 10 kHz and a power density of 0.3 W / cm³. 2 After sedimentation and centrifugation, the extract was subjected to a combination of 10μm and 2μm fine filtration membranes. The filtrate was then filtered through an ultrafiltration membrane (100 kDa) at an operating pressure of 0.1 MPa and a temperature of 25 °C to obtain aqueous extract D.

[0050] S5: The fermentation supernatant C and the water extract D were mixed at a volume ratio of 2:1, pre-frozen at -40 ℃ for 4 h, and then freeze-dried for 20 h to obtain the Dendrobium officinale extract.

[0051] An application of Dendrobium officinale extract, wherein the Dendrobium officinale extract can be used in the field of intestinal anti-inflammatory.

[0052] Example 3

[0053] A method for preparing Dendrobium officinale extract, the specific steps of which are as follows:

[0054] S1: Fresh Dendrobium officinale strips were processed using ultra-low temperature pulverization technology. The pulverization temperature was -90 ℃ and the pulverization time was 90s. The ultra-low temperature pulverization was carried out under the protection of high-purity nitrogen with a nitrogen flow rate of 1 L / min, resulting in material A with a particle size of D50≤50 μm.

[0055] S2: Place material A in PBS buffer at pH 6 to achieve a material-to-liquid ratio of 1:20. Perform enzymatic hydrolysis on material A at 45 °C. Add 10% by mass of a compound enzyme preparation, which is a mixture of cellulase, pectinase, and neutral protease in a mass ratio of 3:1:1. Perform enzymatic hydrolysis at 250 rpm for 6 h. Centrifuge to obtain hydrolysate B and hydrolysis residue.

[0056] S3: Adjust the pH of the enzymatic hydrolysate B to 6.5, sterilize at 115℃ for 15 min, cool to room temperature, and then inoculate with 5% activated Lactobacillus rhamnosus culture. The viable count of the activated Lactobacillus rhamnosus culture should be ≥10. 9 The concentration of CFU / mL was anaerobically cultured at 37℃ for 24 h, followed by centrifugation at 4℃ at 9000 rpm for 20 min. The mixture was then filtered through a 0.22 μm filter to obtain the fermentation supernatant C.

[0057] S4: Extract the enzymatic hydrolysis residue with pure water at a temperature of 60 ℃, a material-to-liquid ratio of 1:8, and an extraction time of 3 h. Simultaneously, use ultrasonic assistance at a frequency of 20 kHz and a power density of 0.5 W / cm³. 2 After sedimentation and centrifugation, the extract was filtered through a combination of 10μm and 2μm fine filtration membranes. The filtrate was then filtered through an ultrafiltration membrane (100 kDa) at an operating pressure of 0.1 MPa and a temperature of 30 °C to obtain aqueous extract D.

[0058] S5: The fermentation supernatant C and the water extract D were mixed at a volume ratio of 4:1, pre-frozen at -40 ℃ for 4 h, and then freeze-dried for 24 h to obtain the Dendrobium officinale extract.

[0059] An application of Dendrobium officinale extract, wherein the Dendrobium officinale extract can be used in the field of intestinal anti-inflammatory.

[0060] Comparative Example 1

[0061] In S2, the compound enzyme preparation is replaced with an equal amount of cellulase, and the remaining steps are the same as in Example 1.

[0062] Comparative Example 2

[0063] In S2, the compound enzyme preparation is replaced with an equal amount of pectinase, and the remaining steps are the same as in Example 1.

[0064] Comparative Example 3

[0065] In S2, the complex enzyme preparation is replaced with an equal amount of neutral protease, and the remaining steps are the same as in Example 1.

[0066] Comparative Example 4

[0067] S3 does not include activated Lactobacillus rhamnosus culture, and the remaining steps are the same as in Example 1.

[0068] Comparative Example 5

[0069] In S4, no ultrafiltration membrane filtration is used; the remaining steps are the same as in Example 1.

[0070] Comparative Example 6

[0071] In S4, the 10μm+2μm fine filtration membrane is not used for combined fine filtration; the remaining steps are the same as in Example 1.

[0072] Comparative Example 7

[0073] Replace the freeze drying in S5 with vacuum drying at 70 °C for 22 h, and the remaining steps are the same as in Example 1.

[0074] Anti-inflammatory activity test

[0075] Experimental procedure: RAW264.7 mouse mononuclear macrophage leukemia cells were selected and seeded in 100 μL of a 5×10⁻⁶ m³ concentration onto a 96-well flat-bottom cell culture plate. 4 Cells were cultured at 37°C, 5% CO2, and above 90% humidity for 24 hours. Afterward, 50 μL of the prepared test compound solution was added, and the cells were cultured under the same conditions. One hour later, 50 μL of the prepared LPS solution (final concentration 1 μg / mL) was added. After 24 hours, 100 μL of supernatant was collected from each well and added to a new 96-well plate. Then, 100 μL of Griess reagent was added to each well, and the mixture was mixed using the cross-hatching method. The absorbance of each well was measured and recorded at 540 nm using a microplate reader. The NO inhibition rate was calculated using the following formula. The control group consisted of commercially available Dendrobium officinale extract purchased from Wuhan Prof Biotechnology Co., Ltd.; the negative control group consisted of DMSO. The test compound was diluted 5 times at 1 / 2 digits. The concentration of the test compound was plotted on the x-axis, and the inhibition rate on the y-axis. The IC50 of the test compound was calculated. 50 value.

[0076] Inhibition rate (%) = (C2-C1) / (C2-C0) × 100%;

[0077] In the formula, C0, C1, and C2 are the absorbance values ​​of the blank control group (without LPS), experimental group, and negative control group (with LPS) measured at 540 nm, respectively. The inhibition rate at each concentration was calculated, and then the half-maximal inhibitory concentration (IC50) of the extract on LPS-induced NO production from RAW264.7 was calculated. 50value).

[0078]

[0079] The experimental data from the examples and comparative examples show that the Dendrobium officinale extract prepared by this invention exhibits strong anti-inflammatory activity and has good application prospects in the field of intestinal anti-inflammatory treatment.

[0080] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention are still within the scope of the present invention.

Claims

1. A method for preparing Dendrobium officinale extract, characterized in that, The specific steps of the preparation method are as follows: S1: Fresh Dendrobium officinale strips were processed using ultra-low temperature pulverization technology. The pulverization temperature was -80~-90 ℃, and the pulverization time was 60~90 s. The ultra-low temperature pulverization was carried out under the protection of high-purity nitrogen with a nitrogen flow rate of 0.5~1 L / min to obtain material A. S2: Place material A in PBS buffer to make the material-to-liquid ratio 1:(10~20), and enzymatically hydrolyze material A at 35~45 ℃. Add 5~10% of the mass of material A with a compound enzyme preparation, and enzymatically hydrolyze at 150~250 rpm for 4~6 h. Centrifuge to separate the enzymatic hydrolysate B and the enzymatic hydrolysis residue. S3: Adjust the pH of the enzymatic hydrolysate B to 6.2-6.5, sterilize at 115℃ for 15 min, cool to room temperature, inoculate with 5% activated Lactobacillus rhamnosus culture, anaerobic culture at 37℃ for 18-24 h, then centrifuge at 4℃, and filter through a 0.22μm filter membrane to obtain fermentation supernatant C; S4: Extract the enzymatic hydrolysis residue with pure water at an extraction temperature of 50-60 ℃, a material-to-liquid ratio of 1:(6-8), and an extraction time of 2-3 h. Simultaneously, turn on ultrasonic assistance. After sedimentation and centrifugation, the extract is filtered through a combination of 10μm and 2μm fine filtration membranes. The fine filtrate is then filtered through an ultrafiltration membrane (100 kDa) at an operating pressure of 0.1 MPa and a temperature of 25-30 ℃ to obtain aqueous extract D. S5: Mix the fermentation supernatant C and the aqueous extract D at a volume ratio of (2~4):1, pre-freeze at -40 ℃ for 4 h, and freeze-dry for 20~24 h to obtain the Dendrobium officinale extract; The S2 compound enzyme preparation consists of cellulase, pectinase, and neutral protease in a mass ratio of (1~3):1:

1.

2. The method for preparing Dendrobium officinale extract according to claim 1, characterized in that, The particle size of Dendrobium officinale in S1 after being treated with ultra-low temperature pulverization technology is D50≤50 μm.

3. The method for preparing Dendrobium officinale extract according to claim 1, characterized in that, The pH of the PBS buffer in S2 is 5-6.

4. The method for preparing Dendrobium officinale extract according to claim 1, characterized in that, The viable bacteria count of the activated Lactobacillus rhamnosus bacterial solution in S3 is ≥10. 9 CFU / mL.

5. The method for preparing Dendrobium officinale extract according to claim 1, characterized in that, In S3, the centrifugation speed is 8000~9000 rpm and the centrifugation time is 10~20 min.

6. The method for preparing Dendrobium officinale extract according to claim 1, characterized in that, The ultrasonic frequency in S4 is 10~20 kHz, and the power density is 0.3~0.5 W / cm². 2 .

7. A Dendrobium officinale extract, prepared by the method described in any one of claims 1 to 6.

8. An application of the Dendrobium officinale extract as described in claim 7, characterized in that, The application of the Dendrobium officinale extract in the preparation of drugs for intestinal anti-inflammatory purposes.

Images