Micro-fluidic chip detection platform

Abstract

The present invention relates to the field of microfluidic chip detection, and particularly discloses a microfluidic chip detection platform, which comprises a disposable joint, a pumping unit and a centrifugation unit, the disposable joint is connected to a sample adding port / liquid adding port of a microfluidic chip, and the disposable joint comprises an isolation paper sheet layer; the pumping unit comprises a micro gas pump capable of instantly generating gas and a pumping port, the disposable joint can be communicated with the pumping port, the micro gas pump can instantly generate micro gas and break through the isolation paper sheet layer to pump a sample / reagent into the micro-fluidic chip, and the centrifugal unit can drive the micro-fluidic chip to centrifugally rotate; and a sample adding port / liquid adding port of the micro-fluidic chip faces the center close to the unit. According to the invention, each detection step of the micro-fluidic chip is realized through image analysis, and the detection result is analyzed. The invention aims to solve the technical problem of how to quickly detect the micro-fluidic chip.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic chip detection, and specifically discloses a microfluidic chip detection platform. Background Art

[0002] Microfluidic chip technology, integrating sample pretreatment, reaction, separation, and detection functions, has become a core technology in point-of-care (POCT) testing. Its core advantages lie in miniaturization, automation, and high throughput, making it widely used in biomedical diagnostics and other fields. However, existing microfluidic chips and detection platforms have shortcomings. For example, sample loading, pumping, and optical detection steps are independent and lack timing coordination, resulting in low efficiency; the pumping accuracy of microsamples is insufficient, resulting in incomplete or slow reactions; and the detection platforms are bulky and difficult to carry.

[0003] The patent application number CN202411067505.X discloses a portable microfluidic chip detection platform, which relates to the technical field of spectral detection equipment. The portable microfluidic chip detection platform includes a mounting mechanism, wherein the mounting mechanism includes a housing and a cover, one end of the cover is hingedly mounted on the top wall of the housing, and the other end of the cover abuts the top wall of the housing; a detection mechanism, wherein the detection mechanism includes a processor, a spectrometer, an air supply assembly and a touch screen, and the processor is fixedly mounted on the bottom wall inside the housing; a drive mechanism, wherein the drive mechanism includes a first telescopic member, a transmission shaft and a transmission frame. This solution ultimately realizes that during the automatic closing of the cover, the telescopic mechanism drives the retraction frame to stably and automatically retract and regulate on the spectrometer, so that the microfluidic chip can be automatically retracted, plugged in and locked during the closing of the cover, preventing loosening and misalignment during manual docking. The structure of the inspection platform is small and easy to carry, but the detection platform should be used for water quality analysis, and its application in the biological field needs to be improved.

[0004] Patent application number CN202221552039.0 discloses a microfluidic detection device and a microfluidic chip. The device includes a main body, which is provided with a chip loading tray for connecting to a microfluidic chip, and the microfluidic chip is used to accommodate an object to be detected and a detection agent; a rotating module is provided on the main body, and the rotating module is connected to the chip loading tray, and is used to control the rotation of the chip loading tray to drive the rotation of the microfluidic chip so that the object to be detected and the detection agent are mixed and a detection reaction occurs; a temperature control module is also provided on the main body, and the temperature control module forms a temperature control cavity for accommodating the microfluidic chip; a control module is provided on the main body, and the control module is communicatively connected to the rotation module, the temperature module and a mobile terminal device, and the control module is used to receive a reaction instruction from the mobile terminal device to control the rotation module and the temperature control module according to the reaction instruction; an observation module is provided on the main body, and the observation module is located in a specified direction of the microfluidic chip, and the observation module is used to observe the detection reaction between the object to be detected and the detection agent.

[0005] The above patent provides a small microfluidic chip detection platform, which uses centrifugation to fuse the sample to be tested with the reagent, and detects it through a camera. It is also equipped with a temperature control module and has complete functions. It is a good microfluidic detection device. However, the above patent also has some problems, such as: 1. The flip-top structure will cause an increase in dust and requires frequent cleaning; 2. The simple centrifugal type has a relatively long centrifugation time, which increases the detection time; 3. Its microfluidic chip is an integrated disc. The microfluidic chip can detect multiple samples at a time, but adding samples, adding reaction reagents, and extracting waste liquid are relatively troublesome, and it does not have the universality to detect other brands of chips. New and old samples may also be contaminated with each other during the detection process. Summary of the Invention

[0006] In view of this, the object of the present invention is to provide a microfluidic chip detection platform to solve the technical problem of how to quickly detect microfluidic chips.

[0007] In order to achieve the above object, the present invention provides the following technical solutions: A microfluidic chip detection platform includes a disposable connector, a pumping unit, and a centrifugal unit. The disposable connector is connected to a sample port and a liquid adding port of the microfluidic chip, and includes an isolation paper layer. The pumping unit includes a micro air pump capable of instantaneously emitting gas and a pumping port. The disposable connector can be connected to the pumping port. The micro air pump can instantaneously emit a trace amount of gas and break through the isolation paper layer to pump samples / reagents into the microfluidic chip. The centrifugal unit can drive the microfluidic chip to rotate centrifugally. The sample port and liquid adding port of the microfluidic chip are oriented near the center of the unit.

[0008] This solution combines the two methods of centrifugation and micro-air pumping. The sample and reagent to be tested are first pumped into the channel of the microfluidic chip by a micro-air pump, and then the mixing of the reagent and sample is accelerated by centrifugation to complete the detection. In the prior art, when adding samples, the sample is generally dripped into the corresponding sample addition hole, and then the sample is pumped into the microfluidic chip by a micro-pump. This operation will contaminate the pumping pipeline, causing the sample to be contaminated in the pipeline, making the cleaning of the pipeline more complicated. In this solution, the sample and reagent are dripped into the sample addition port and the liquid addition port respectively, and then pumped by a micro-air pump. In this way, the pumping pipeline does not come into contact with the sample and reagent, which can avoid this problem.

[0009] Optionally, it further includes a housing, the outer side of which is provided with a slot into which a chip tray can be inserted, a chip tray is placed on the chip tray, and the chip tray is provided with a circular groove and an installation card strip for installing a microfluidic chip.

[0010] Optionally, the pumping unit further comprises a descending cylinder and a pumping plate, the pumping plate being fixedly mounted on the telescopic end of the descending cylinder, the pumping port being disposed on the pumping plate, and the pumping port being connected to the micro air pump. In this embodiment, the pumping plate and descending cylinder are both disposed on the upper portion of the housing and are moved downward from the upper portion to engage the pumping port and the disposable connector. This not only facilitates the engagement, but also allows the entire chip tray to be pressed against the chip insert, facilitating subsequent connection between the microcentrifuge and the chip tray.

[0011] Optionally, the centrifugal unit includes an ascending cylinder, a microcentrifuge, and a fixed joint. The telescopic end of the ascending cylinder is provided with a mounting platform, the microcentrifuge is mounted on the mounting platform, and the fixed joint is fixedly mounted on the output end of the microcentrifuge. The fixed joint is circular in shape. The ascending cylinder can drive the microcentrifuge upward, allowing the fixed joint to engage with the circular groove of the chip tray, while also allowing the chip tray to be separated from the chip insert to avoid friction during centrifugation.

[0012] Optionally, the device further includes a lighting unit and a camera unit. The lighting unit includes a light panel in a circular array, the light panel being fixedly mounted at the bottom of the housing. The camera unit includes at least two cameras, each located at the top of the housing. In this embodiment, positioning the camera unit at the bottom provides better lighting, facilitating the camera's ability to capture images for analysis.

[0013] Optionally, the cameras are arranged uniformly along the top of the housing; the cameras are arranged at an angle. Providing two cameras and tilting them allows all microfluidic chips to be captured, and the tilting angle facilitates image analysis, such as color band analysis and positioning analysis.

[0014] Optionally, the microfluidic chip includes a sample inlet and a liquid inlet, each of which is connected to a container, which is threadedly connected to the bottom of the disposable connector. This solution provides a simple and convenient threaded connection, and after tightening, the top of the disposable connector remains at the same height, facilitating subsequent connection between the disposable connector and the pumping port.

[0015] Optionally, a ring-shaped ribbon is provided in the middle of the disposable connector, and the pumping port is a different color than the ribbon. The pumping port can be inserted into the disposable connector, obscuring the ribbon. With this solution, a camera can be used to detect whether the ribbon is obscured. If the ribbon is obscured, the connection is complete and the seal is good, allowing gas pumping to proceed.

[0016] A detection method for a microfluidic chip detection platform comprises the following steps: S1, sampling, drop the sample to be tested into the sample port of the microfluidic chip, drop the added reagent into the liquid addition port, and screw the disposable connector into the sample port and liquid addition port; S2, loading, mounting all microfluidic chips on the chip tray, mounting the chip tray on the chip insert, and inserting the chip insert into the slot of the housing; S3, pumping, the camera determines the position of the microfluidic chip through image analysis. When the position of the microfluidic chip is accurate, the downward cylinder is started, and the downward cylinder drives the pumping disk to move downward. The pumping port on the pumping disk is plugged into the disposable connector. The stroke of the downward cylinder is fixed. After the downward cylinder stroke is in place, the camera determines whether the ribbon of the disposable connector is completely covered through image analysis. If the ribbon is not completely covered, the downward cylinder continues to drive the pumping disk to move downward 2-3mm and then perform image analysis again. When the ribbons of all disposable connectors are covered, the micro air pump is started. The micro air pump instantly pumps the gas into the disposable connector, and then the gas breaks through the isolation paper layer in the disposable connector to pump the sample and reagent to be tested into the microfluidic chip. S4, centrifugation, start the upward cylinder, the upward cylinder drives the micro centrifuge to move upward until the fixed joint on the micro centrifuge fits into the circular groove of the chip tray, and the stroke of the upward cylinder is fixed; start the downward cylinder, the downward cylinder drives the pumping plate to move upward to the original position; start the micro centrifuge, the speed is 1500-2500 rpm, and the centrifugation time is 30-90 seconds; S5, image analysis, the camera analyzes the microfluidic chip through images and determines the detection results.

[0017] The working principle and beneficial effects of this solution are: The main structures in this solution include a disposable connector, a pumping unit and a centrifugal unit. The disposable connector is connected to the microfluidic chip and is mainly used to isolate the sample port and liquid port on the microfluidic chip to prevent the leakage of samples and reagents when transporting the microfluidic chip. At the same time, it can also enable the microfluidic chip to be connected to the pumping unit, making it convenient for the pumping unit to pump samples and reagents into the channels of the microfluidic chip. The pumping time is fast, but the pumping effect is relatively poor, and uneven mixing may occur. The centrifugal unit can drive the microfluidic chip to rotate centrifugally, further helping to mix the samples and reagents in the microfluidic chip. When the mixing is completed, the image analysis is performed through the camera to complete the detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural diagram of an embodiment; Figure 2 for Figure 1 Structural diagram from another perspective; Figure 3 This is a partial structural diagram of the power supply and control unit inside the left chamber of the casing; Figure 4 Schematic diagram of the internal structure of the right chamber; Figure 5 It is a partial structural diagram of the right chamber; Figure 6 It is a structural diagram of the upward cylinder and the micro centrifuge; Figure 7 Schematic diagram of the structure of the chip insert, chip tray, microfluidic chip and disposable connector; Figure 8 Schematic diagram of the structure of the microfluidic chip and disposable connector; Figure 9 It is a structural diagram of the descending cylinder and the pumping plate; Figure 10 Schematic diagram of the internal structure of the pipeline cavity.

[0019] The markings in the accompanying drawings are as follows: casing 1, left side panel 2, front panel 3, top panel 4, conical cavity 5, display unit 6, right side panel 7, slot 8, rear panel 9, left chamber 10, power supply 11, control unit 12, right chamber 13, lighting unit 14, ascending cylinder 15, mounting platform 16, micro centrifuge 17, support platform 18, support plate 19, chip insert 20, chip tray 21, microfluidic chip 22, camera 23, micro air pump 24, main pipeline 25, branch pipeline 26, descending cylinder 27, pumping disk 28, fixed joint 29, placement groove 30, positioning groove 31, circular groove 32, bracket 33, elastic sleeve 34, limit frame 35, installation card strip 36, filling tube 37, disposable joint 38, isolation paper layer 39, ribbon 40, pumping port 41, support plate 42, partition 43, elastic pull ring 44. DETAILED DESCRIPTION

[0020] The following is further described in detail through specific implementation methods: Example

[0021] Microfluidic chip 22, such as Figures 1-10 As shown, specifically Figure 7 、 Figure 8 As shown, it includes a chip body, which is provided with a liquid adding port and a sample adding port. The liquid adding port and the sample adding port are connected and integrated with a containing cylinder 37. The internal chamber of the containing cylinder 37 is stepped, and the lower inner diameter of the internal chamber is smaller than the upper inner diameter. The upper end of the containing cylinder 37 is provided with an external thread.

[0022] The disposable connector 38 can be connected to the container 37. The disposable connector 38 is cylindrical. The lower end of the disposable connector 38 is provided with an internal thread, which can be connected to the external thread of the container 37. The upper end of the disposable connector 38 has a small diameter, and a color ribbon 40 is formed at the bottom of its upper end.

[0023] A microfluidic chip 22 detection platform, combined with Figure 1-5 , 7-10, including a housing 1, a display unit 6, a pumping unit, a centrifugal unit, a control unit 12 and an inspection unit.

[0024] The casing 1 is in the shape of a rectangular parallelepiped as a whole and has an inclined surface. A rack is provided inside the casing 1. The front panel 3, rear panel 9, right side panel 7, left side panel 2 and top panel 4 of the casing 1 are all mounted on the rack and can be removed. A conical cavity 5 is formed on the top of the casing 1, and the conical cavity 5 is used to install components of the detection platform. A slot 8 is provided on the right side panel 7, and the inspection unit can be inserted into the slot 8. The rack is divided into a left chamber 10 and a right chamber 13, wherein the left chamber 10 is used to install the control unit 12, and the right chamber 13 is used to install the pumping unit, the centrifugal unit and the inspection unit. The lower ends of the left chamber 10 and the right chamber 13 are connected, mainly for arranging lines, and the equipment power supply 11 is generally arranged in the left chamber 10.

[0025] The display unit 6 includes a display screen and an operation keyboard, and both the display screen and the operation keyboard are arranged on the inclined surface of the housing 1 .

[0026] The pumping unit includes a trace air pump 24, a descending cylinder 27 and a pumping disc 28. The descending cylinder 27 is fixed in the conical chamber of the frame by bolts. The pumping disc 28 is fixedly connected to the output end of the descending cylinder 27. There are a number of pumping ports 41 in a circular array on the pumping disc 28. The pumping ports 41 are located at the outer end of the pumping disc 28. The lower end of the pumping port 41 extends out and can be externally inserted into the upper end of the disposable connector 38 and cover the ribbon 40. The outer surface color of the lower end of the pumping port 41 is different from that of the ribbon 40. Several support plates 42 are integrally formed on the top of the interior of the frame. A pipe cavity is formed in the support plate 42. The trace air pump 24 is fixed on the frame, and the trace air pump 24 is connected to the main pipeline 25. Combined Figure 4 、 5 10. The conduit cavity is provided with a number of staggered baffles 43, arranged sequentially from top to bottom and staggered left to right. Resilient pull rings 44 are located at opposing positions on each baffle 43. These are typically made of rubber and secured by hot melt. After entering the conduit cavity, the main conduit 25 meanders along the baffles 43 and penetrates each elastic pull ring 44. The main conduit 25 is then directly secured to the pumping disc 28. Several branch conduits 26 are located at the upper end of the pumping disc 28. These branch conduits 26 communicate with corresponding pumping ports 41. All branch conduits 26 converge and communicate with the main conduit 25.

[0027] The inspection unit includes a support platform 18, a chip tray 21, a chip insert 20, and a slot 8. Slot 8 is located on the right side panel 7 of the housing 1. A support plate 19 is also horizontally positioned below slot 8 within the right side panel 7. The support platform 18 is located on the left side of the housing and primarily supports the chip insert 20. A circular placement slot 30 is formed on the chip insert 20. The placement slot 30 is larger than the chip tray 21 and is used to accommodate the chip tray 21. The placement slot 30 also includes three smaller positioning slots 31 arranged in a uniform circular pattern. A circular slot 32 is located at the center of the chip tray 21. Several sets of mounting clips 36 are provided on the chip tray 21. Each set of mounting clips 36 has four elastic, rectangular, L-shaped fixing strips. The four fixing strips form a rectangle that is the same size as the microfluidic chip 22. A positioning block is provided at the bottom of the chip tray 21 to mate with the positioning slots 31.

[0028] Combine Figure 5-6 As shown, the centrifugal unit includes an ascending cylinder 15, a microcentrifuge 17, and a fixed joint 29. The ascending cylinder 15 is fixed to the bottom of the frame. A mounting platform 16 is fixed to the telescopic end of the ascending cylinder 15. The microcentrifuge 17 is mounted on the mounting platform 16, and a gasket for shock absorption and isolation is provided between the microcentrifuge 17 and the mounting platform 16. Two limiters 35 are provided between the microcentrifuge 17 and the mounting platform 16. The limiters 35 are used to prevent the microcentrifuge 17 from getting too close to the chip tray 21, thereby preventing the fixed joint 29 from dislodging from the upper end of the circular groove 32. The fixed joint 29 is fixed to the output end of the microcentrifuge 17 and is circular in shape. The fixed joint 29 is adapted to engage the circular groove 32 of the chip tray 21. The outer adhesive sleeve of the fixed joint 29 is provided with an elastic sleeve 34. The elastic sleeve 34 is generally gourd-shaped, with protrusions at the upper and lower ends and a recessed center.

[0029] The lighting unit 14 includes a light board in a circular array, which is fixedly arranged at the lower part of the frame, surrounds the centrifugal unit and is aligned with the chip tray 21. The upper end surface of the light board is inclined toward the center.

[0030] The camera unit includes three cameras 23, each of which is located at the top of the housing 1. The three cameras 23 are evenly arrayed in a ring along the top of the housing 1, and the cameras 23 are arranged at an angle.

[0031] A detection method for a microfluidic chip 22 detection platform comprises the following steps: S1, sampling, dripping the sample to be tested into the sample addition port of the microfluidic chip 22, dripping the additional reagent into the liquid addition port, and screwing the disposable connector 38 into the sample addition port and the liquid addition port; using this method to add samples and add liquid is relatively simple. After screwing in the disposable connector, it can prevent the sample / reagent from leaking during transportation, and it can also prevent the sample / reagent from being thrown out during centrifugation to a certain extent.

[0032] S2, loading, install all microfluidic chips 22 on the chip tray 21 and fix them through the mounting clip 36, install the chip tray 21 on the chip insert 20, and insert the chip insert 20 into the slot 8 of the housing 1; during the process, the positioning block of the chip tray 21 needs to cooperate with the positioning groove 31.

[0033] S3, pumping, the camera 23 determines the position of the microfluidic chip 22 through image analysis. When the position of the microfluidic chip 22 is accurate, the downward cylinder 27 is started, and the downward cylinder 27 drives the pumping disk 28 to move downward. The pumping port 41 on the pumping disk 28 is plugged into the disposable connector 38. The stroke of the downward cylinder 27 is fixed. After the downward cylinder 27 has reached its stroke, the camera 23 determines whether the ribbon 40 of the disposable connector 38 is completely covered through image analysis. If the ribbon 40 is not completely covered, the downward cylinder 27 continues to drive the pumping disk 28 downward. After moving 2-3 mm, the image analysis and judgment are performed again. When the ribbons 40 of all disposable connectors 38 are covered, the micro air pump 24 is started. The micro air pump 24 instantly pumps gas into the disposable connector 38, and then the gas breaks through the isolation paper layer in the disposable connector 38, and pumps the sample to be tested and the reagent into the microfluidic chip 22; the telescopic end of the downward cylinder will drive the main pipe to move downward during the movement. At this time, the main pipe moves downward in the pipe cavity, and the elastic pull ring moves. When the downward cylinder returns to its position, the elastic pull ring can pull the main pipe back to its original position. S4, centrifugation, start the upward cylinder 15, the upward cylinder 15 drives the micro centrifuge 17 to move upward until the fixed joint 29 on the micro centrifuge 17 cooperates with the circular groove 32 of the chip tray 21, and the stroke of the upward cylinder 15 is fixed; start the downward cylinder 27, the downward cylinder 27 drives the pumping disk 28 to move upward to its original position; start the micro centrifuge 17, the speed is 1500-2500rpm, and the centrifugation time is 30-90s; during the upward movement of the fixed joint, the chip tray is pressed against the chip insert, so even if the size of the elastic sleeve is slightly larger than the circular groove, the elastic sleeve can be inserted into the circular groove and have an interference fit with the circular groove.

[0034] S5, image analysis: the camera 23 analyzes the microfluidic chip 22 through images and determines the detection results.

[0035] The above description is merely an embodiment of the present invention. Common knowledge regarding the specific structure and characteristics of the solution is not described in detail herein. It should be noted that those skilled in the art will be able to make various modifications and improvements without departing from the structure of the present invention. These modifications and improvements should also be considered within the scope of protection of the present invention and will not affect the effectiveness and practicality of the present invention.

Claims

1. A microfluidic chip detection platform, characterized by: The device comprises a disposable connector, a pumping unit and a centrifugal unit. The disposable connector is connected to the sample port and liquid adding port of the microfluidic chip, and the disposable connector includes an isolation paper layer. The pumping unit includes a micro air pump capable of instantaneously emitting gas and a pumping port. The disposable connector can be connected to the pumping port. The micro air pump can instantaneously emit a trace amount of gas and break through the isolation paper layer to pump the sample / reagent into the microfluidic chip. The centrifugal unit can drive the microfluidic chip to rotate centrifugally. The sample port and liquid adding port of the microfluidic chip are oriented near the center of the unit.

2. A microfluidic chip detection platform according to claim 1, characterized in that: The invention also includes a housing, wherein the outer side of the housing is provided with a slot into which a chip insert is inserted, a chip tray is placed on the chip insert, and a circular groove and an installation card strip for installing a microfluidic chip are provided on the chip tray.

3. A microfluidic chip detection platform according to claim 2, characterized in that: The pumping unit further comprises a descending cylinder and a pumping disc, wherein the pumping disc is fixedly arranged on the telescopic end of the descending cylinder, and the pumping port is arranged on the pumping disc, and the pumping port is communicated with the micro air pump.

4. A microfluidic chip detection platform according to claim 3, characterized in that: The centrifugal unit includes an ascending cylinder, a micro centrifuge and a fixed joint. The telescopic end of the ascending cylinder is provided with a mounting platform. The micro centrifuge is arranged on the mounting platform. The fixed joint is fixedly arranged on the output end of the micro centrifuge and is circular.

5. A microfluidic chip detection platform according to claim 4, characterized in that: It also includes a lighting unit and a camera unit. The lighting unit includes a light board in a ring array, and the light board is fixedly arranged at the lower part of the casing. The camera unit includes at least two cameras, and the cameras are located at the top of the casing.

6. The microfluidic chip detection platform according to claim 5, characterized in that: The cameras are arranged evenly along the top of the housing; and the cameras are arranged tilted.

7. A microfluidic chip detection platform according to any one of claims 1 to 6, characterized in that: The microfluidic chip comprises a sample adding port and a liquid adding port, both of which are connected with a containing cylinder, and the containing cylinder is threadedly connected to the bottom of the disposable joint.

8. The microfluidic chip detection platform according to claim 7, characterized in that: A ring-shaped color ribbon is provided in the middle of the disposable connector, the color of the pumping port is different from the color ribbon, and the pumping port can be inserted into the disposable connector, and the pumping port can cover the color ribbon.

9. The detection method of a microfluidic chip detection platform according to claim 8, characterized in that: The following steps are involved: S1, sampling, drop the sample to be tested into the sample port of the microfluidic chip, drop the added reagent into the liquid addition port, and screw the disposable connector into the sample port and liquid addition port; S2, loading, mounting all microfluidic chips on the chip tray, mounting the chip tray on the chip insert, and inserting the chip insert into the slot of the housing; S3, pumping, the camera determines the position of the microfluidic chip through image analysis. When the position of the microfluidic chip is accurate, the downward cylinder is started, and the downward cylinder drives the pumping disk to move downward. The pumping port on the pumping disk is plugged into the disposable connector. The stroke of the downward cylinder is fixed. After the downward cylinder stroke is in place, the camera determines whether the ribbon of the disposable connector is completely covered through image analysis. If the ribbon is not completely covered, the downward cylinder continues to drive the pumping disk to move downward 2-3mm and then perform image analysis again. When the ribbons of all disposable connectors are covered, the micro air pump is started. The micro air pump instantly pumps the gas into the disposable connector, and then the gas breaks through the isolation paper in the disposable connector to pump the sample and reagent to be tested into the microfluidic chip. S4, centrifugation, start the upward cylinder, the upward cylinder drives the micro centrifuge to move upward until the fixed joint on the micro centrifuge fits into the circular groove of the chip tray, and the stroke of the upward cylinder is fixed; start the downward cylinder, the downward cylinder drives the pumping plate to move upward to the original position; start the micro centrifuge, the speed is 1500-2500 rpm, and the centrifugation time is 30-90 seconds; S5, image analysis, the camera analyzes the microfluidic chip through images and determines the detection results.

Images