Elastic loading device for micro-fluidic chip

Abstract

The invention discloses an elastic loading device for a micro-fluidic chip. The elastic loading device comprises a clamping seat, a clamping cover, a spring mechanism and a rubber strip, the clamping seat comprises a first head section, a first middle section and a first tail section; a first trapezoidal groove is formed in one side, close to the first head section, of the first tail section; the clamping cover comprises a second head section, a second middle section and a second tail section; a rectangular space is formed between the first head section and the second head section; a sliding through hole is formed in one side, close to the first tail section, of the rectangular space; the spring mechanism comprises a sliding block, a spring and a sliding sheet, and the sliding block is clamped into the rectangular space; one end of the spring is fixedly connected to the first head section, and the other end is connected with the sliding block; one side of the sliding block transversely penetrates through the sliding through hole; the sliding piece comprises a sliding piece front plate and a sliding piece rear plate. The sliding block penetrates through the sliding through hole to drive the sliding piece to slide in the length direction of the first head section. The rubber strip comprises a fixed end and a movable end. The problems that clamping is inconvenient, and too much time is wasted when a large number of samples are detected can be solved; and the chip cannot be stably supported.

Description

Technical Field

[0001] The present invention relates to the technical field of PCR (Polymerase Chain Reaction) microfluidic chips, and more particularly to an elastic loading device for a microfluidic chip. Background Art

[0002] The PCR microfluidic chip is a device that combines polymerase chain reaction (PCR) technology with microfluidics, featuring integration, automation, miniaturization, and portability. It is widely used in pathogen detection, gene mutation detection, food safety, and clinical diagnosis. The loading device of the PCR microfluidic chip is a key component for achieving stable chip fixation and precise operation. The elastic loading device of the microfluidic chip is a device used to fix and operate the microfluidic chip. It utilizes the properties of elastic materials to achieve stable loading of the PCR chip, thereby improving operational convenience and ensuring experimental accuracy. It plays a significant role in achieving rapid testing.

[0003] Chinese patent application document 1 (application number: 2024113676800, application date: 2024.09.29) discloses a loading device and nucleic acid detection equipment for microfluidic chips, including: at least one chip tray 6' and a hollow positioning frame 7', each chip tray 6' is detachably loaded with a microfluidic chip, at least one side wall or two opposite side walls of the positioning frame 7' is provided with at least one tray slot 28', each chip tray 6' is inserted through a tray slot 28' and is confined to a fixed position in the hollow area of ​​the positioning frame 7', and an openable side cover 5' is provided on the outer side of the side wall of the positioning frame 7' with the tray slot 28', and at least one group of elastic components is provided through the side wall of the side cover 5', which is used to apply pressure to the end face 23' of each chip tray 6' when the side cover 5' is closed to compress the microfluidic chip. The above-mentioned scheme is cumbersome to engage, which is not conducive to the rapid loading of a large number of microfluidic chips, thus having obvious limitations when testing a large number of samples; further, the closure of the support plate slot 28' is tightened by screws, and the closing method is cumbersome, further reducing the sample loading speed and increasing the detection time; in addition, there is no support on the side of the support plate 6' away from the support plate slot 28', so that this side of the support plate 6' cannot remain continuously stable during operation.

[0004] Chinese patent application document 2 (application number: 2019224500426, application date: 2019.12.30) discloses a digital PCR chip holder, including a chip holder, a card holder, a chip and a heat dissipation fan housing. A first mounting cavity is provided in the center of the chip holder, and a second mounting cavity is provided on one side wall of the chip holder, and the second mounting cavity is connected to the first mounting cavity. A first fixing groove is provided at the first mounting cavity, and sliding grooves are provided on both sides of the first mounting cavity. A plurality of springs are provided at the bottom end of the first mounting cavity, and the top end of the spring is connected to the bottom of the support plate. The above scheme cannot facilitate observation while performing rapid testing. Furthermore, the second mounting cavity and the first mounting cavity are too densely arranged, and the heat dissipation air cannot be fully blown to dissipate heat. In addition, the circulation of the cold air is poor, and the heat dissipation effect is poor.

[0005] Chinese patent application document 3 (application number: 2022204160870, application date: 2022.02.26) discloses a cartridge device for fixing a microfluidic chip, including a flip cover and a base, wherein the flip cover and the base are rotatably connected by a rotating shaft, and a chip card slot for placing the microfluidic chip is provided on the base; a first connecting member and a second connecting member that cooperate with each other are also provided between the flip cover and the base, wherein the first connecting member is installed on the flip cover and the second connecting member is installed on the base. The above scheme needs to be kept horizontal to achieve the cover fixation, which is very picky about the use environment; furthermore, because the above cartridge is provided with a positioning hole and the chip is fully sealed, the sample addition will inevitably cause contamination to the cartridge, and it cannot be reused by replacing the chip.

[0006] Therefore, it is a technical problem that urgently needs to be solved in this field to provide an elastic loading device for microfluidic chips that can quickly and conveniently engage the microfluidic chip, has obvious and significant advantages when testing a large number of samples, and can stably fix and support the chip to reduce the operational risks during the testing process. Summary of the Invention

[0007] In view of this, the present invention provides an elastic loading device for a microfluidic chip, which can solve the problems of inconvenient engagement, significant waste of time in testing a large number of samples, and the inability to stably fix and support the chip, thereby failing to reduce the operational risks during the testing process.

[0008] The present application provides an elastic loading device for a microfluidic chip, comprising a card seat, a card cover, a spring mechanism and a rubber strip; wherein,

[0009] The card holder upper cover is combined with a card cover, which includes a first head section, a first middle section, and a first tail section connected in sequence. A first U-shaped structure is formed among the first head section, the first middle section, and the first tail section; on one side of the first tail section close to the first head section, a first trapezoidal groove recessed away from the first head section is provided, and the first trapezoidal groove extends along the length direction of the first head section; on one side of the first head section close to the card cover, a rectangular lower groove recessed away from the card cover is provided, and the rectangular lower groove extends along the length direction of the first head section; along the direction from the card cover to the card holder, the cross-sectional shape of the first head section is an inverted F shape;

[0010] The card cover includes a second head section, a second middle section, and a second tail section connected in sequence. A second U-shaped structure is formed among the second head section, the second middle section, and the second tail section. The openings of the first U-shaped structure and the second U-shaped structure both face the rubber strip side; along the direction from the card cover to the card holder, the orthographic projections of the second head section, the second middle section, and the second tail section respectively coincide within the orthographic projections of the first head section, the first middle section, and the first tail section; on one side of the second head section close to the first head section, a rectangular upper groove recessed away from the first head section is provided; the openings of the rectangular upper groove and the rectangular lower groove face each other. When the first head section and the second head section are covered, the rectangular upper groove and the rectangular lower groove form a rectangular space; on one side of the rectangular upper groove and the rectangular lower groove close to the first tail section, sliding through holes penetrating along the direction from the first head section to the first tail section are respectively provided, and the sliding through holes extend along the length direction of the first head section, and the sliding through holes are rectangular in shape;

[0011] The spring mechanism includes a slider, a spring, and a sliding plate. The slider is snap-fitted into the rectangular space; the slider includes an L-shaped slider and a Z-shaped slider integrally connected to the L-shaped slider; one ends of the L-shaped slider and the Z-shaped slider face the opening of the first U-shaped structure, and the other ends thereof are away from the opening of the first U-shaped structure; the orthographic projection shape of the Z-shaped slider along the direction from the first head section to the first tail section is Z-shaped, and its orthographic projection shape along the direction from the card cover to the card holder is rectangular; one end of the spring is fixedly connected to the end of the first head section far from the first middle section, and the other end thereof is connected to one side of the Z-shaped slider close to the opening of the first U-shaped structure; the orthographic projection shape of the L-shaped slider along the length direction of the first head section is an inverted L shape, and one side of the L-shaped slider away from the Z-shaped slider penetrates through the sliding through hole;

[0012] The slide comprises a slide front plate and a slide rear plate connected to the slide front plate, an L-shaped structure is formed between the slide front plate and the slide rear plate, the slide front plate is parallel to the first head section, the slide rear plate is parallel to the first middle section, the side of the slide front plate close to the first head section is fixedly connected to the side of the L-shaped slider close to the first tail section, the Z-shaped slider and the L-shaped slider pass through the sliding through hole to drive the slide to slide along the length direction of the first head section, a trapezoidal hole is opened on the slide front plate, the trapezoidal hole passes through the slide front plate in the direction from the first head section to the first tail section, and extends along the length direction of the first head section, the microfluidic chip is elastically snapped into place between the first trapezoidal groove and the trapezoidal hole, the trapezoidal hole has a lower bottom surface and an upper bottom surface, along the direction of the card cover pointing to the card seat, the height of the lower bottom surface is greater than the height of the upper bottom surface, and the lower bottom surface is flush with the slide rear plate;

[0013] The rubber strip is arranged parallel to the first middle section and includes a fixed end and a movable end at both ends along its length direction; the fixed end is fixedly glued to one end of the second tail section away from the second middle section, and the movable end is buckled to one side of the rectangular space away from the second middle section.

[0014] Compared with the prior art, the elastic loading device for a microfluidic chip provided by the present invention achieves at least the following beneficial effects:

[0015] First, through the elastic loading device for microfluidic chips provided by this embodiment, the microfluidic chip can be quickly and conveniently engaged, which has obvious and significant advantages when testing a large number of samples; first, the card holder provided by this embodiment is provided with a first trapezoidal groove, and the card holder is made of rubber, which can elastically engage the microfluidic chip on one side along its length direction; further, the slide provided by this embodiment has a trapezoidal hole, which can elastically engage the microfluidic chip on the other side along its length direction; this engaging is automatically completed during the loading process of the microfluidic chip, and can be achieved without additional fixing, which facilitates the rapid loading of a large number of chips; in addition, the rubber strip provided by this embodiment can complete the sealing operation by buckling after the microfluidic chip is loaded, which further saves loading time.

[0016] Second, the elastic loading device for the microfluidic chip provided in this embodiment can stably support the chip, thereby reducing the operational risks during the detection process; first, the first trapezoidal groove and trapezoidal hole provided in this embodiment directly support and fix the microfluidic chip from both sides along its width direction, making the fixation of the microfluidic chip more stable; secondly, the slide back plate and rubber strip provided in this embodiment clamp and fix the microfluidic chip from both sides along its length direction. Combined with the first trapezoidal groove and trapezoidal hole, the four sides of the microfluidic chip along its circumferential direction are fixed; the firmly fixed microfluidic chip can reduce the operational risks during the detection process; in addition, the loading and disassembly of the microfluidic chip only requires contact with the side of the microfluidic chip along its circumferential direction, which can reduce the risk of contamination on the chip surface.

[0017] Of course, any product implementing the present invention does not necessarily need to achieve all of the technical effects described above at the same time.

[0018] Further features and advantages of the present invention will become apparent from the following detailed description of exemplary embodiments of the present invention with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and, together with the description, serve to explain the principles of the invention.

[0020] Figure 1 This is a schematic diagram of a loading device and nucleic acid detection device for a microfluidic chip provided in Chinese patent application document 1;

[0021] Figure 2 This is a schematic diagram of the disassembled structure of an elastic loading device for a microfluidic chip provided by the present invention;

[0022] Figure 3 Schematic diagram of the structure of an elastic loading device for a microfluidic chip provided by the present invention;

[0023] Figure 4 This is a schematic diagram of the assembly of the card holder and the fixing plate provided by the present invention;

[0024] Figure 5 This is a bottom view of the card cover provided by the present invention;

[0025] Figure 6 It is a structural schematic diagram of the spring mechanism provided by the present invention;

[0026] Figure 7 It is a structural schematic diagram of the slider provided by the present invention;

[0027] Figure 8 is a top view of the slider provided by the present invention;

[0028] Figure 9 Schematic diagram of the structure of the rubber strip provided by the present invention;

[0029] Figure 10 yes Figure 2 A magnified view of point A in the figure;

[0030] Figure 11 It is a partial structural diagram of the first head section, the first fixed cylinder and the triangular prism provided by the present invention;

[0031] Figure 12 yes Figure 3 Enlarged view of point B in . DETAILED DESCRIPTION

[0032] Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. It should be noted that unless otherwise specifically stated, the relative arrangement of components and steps, numerical expressions and numerical values ​​set forth in these embodiments do not limit the scope of the present invention.

[0033] The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses.

[0034] Technologies, methods, and equipment known to ordinary technicians in the relevant art may not be discussed in detail, but where appropriate, the technologies, methods, and equipment should be considered part of the specification.

[0035] In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not limiting. Therefore, other examples of the exemplary embodiments may have different values.

[0036] It should be noted that like reference numerals and letters refer to like items in the following figures, and therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0037] Example 1

[0038] Reference Figure 2-Figure 9 As shown, Figure 2 This is a schematic diagram of the disassembled structure of an elastic loading device for a microfluidic chip provided by the present invention; Figure 3 Schematic diagram of the structure of an elastic loading device for a microfluidic chip provided by the present invention; Figure 4 This is a schematic diagram of the assembly of the card holder and the fixing plate provided by the present invention; Figure 5 This is a bottom view of the card cover provided by the present invention; Figure 6 It is a structural schematic diagram of the spring mechanism provided by the present invention; Figure 7 It is a structural schematic diagram of the slider provided by the present invention; Figure 8 is a top view of the slider provided by the present invention; Figure 9 This embodiment provides an elastic loading device for a microfluidic chip, comprising a card seat 1, a card cover 2, a spring mechanism 4 and a rubber strip 3; wherein,

[0039] A card cover 2 is covered on the card seat 1. It includes a first head section 11, a first middle section 12, and a first tail section 13 connected in sequence. A first U-shaped structure is formed among the first head section 11, the first middle section 12, and the first tail section 13; on one side of the first tail section 13 close to the first head section 11, a first trapezoidal groove 131 recessed in the direction away from the first head section 11 is provided, and the first trapezoidal groove 131 extends along the length direction Y of the first head section 11; on one side of the first head section 11 close to the card cover 2, a rectangular lower groove 111 recessed in the direction away from the card cover 2 is provided, and the rectangular lower groove 111 extends along the length direction Y of the first head section 11; along the direction Z from the card cover 2 to the card seat 1, the cross-sectional shape of the first head section 11 is an inverted F shape;

[0040] The card cover 2 includes a second head section 21, a second middle section 22, and a second tail section 23 connected in sequence. A second U-shaped structure is formed among the second head section 21, the second middle section 22, and the second tail section 23. The openings of the first U-shaped structure and the second U-shaped structure both face the side of the rubber strip 3; along the direction Z from the card cover 2 to the card seat 1, the orthographic projections of the second head section 21, the second middle section 22, and the second tail section 23 respectively coincide within the orthographic projections of the first head section 11, the first middle section 12, and the first tail section 13; on one side of the second head section 21 close to the first head section 11, a rectangular upper groove 211 recessed in the direction away from the first head section 11 is provided; the openings of the rectangular upper groove 211 and the rectangular lower groove 111 face each other. When the first head section 11 and the second head section 21 are covered, the rectangular upper groove 211 and the rectangular lower groove 111 form a rectangular space; on one side of the rectangular upper groove 211 and the rectangular lower groove 111 close to the first tail section 13, sliding through holes 212 penetrating along the direction X from the first head section 11 to the first tail section 13 are provided respectively. The sliding through holes 212 extend along the length direction Y of the first head section 11, and the sliding through holes 212 are rectangular in shape;

[0041] The spring mechanism 4 includes a slider 42, a spring 41, and a sliding piece 43. The slider 42 is clamped into the rectangular space; the slider 42 includes an L-shaped slider 421 and a Z-shaped slider 422 integrally connected to the L-shaped slider 421; one ends of the L-shaped slider 421 and the Z-shaped slider 422 face the opening of the first U-shaped structure, and the other ends thereof face away from the opening of the first U-shaped structure; the orthographic projection shape of the Z-shaped slider 422 along the direction X from the first head section 11 to the first tail section 13 is Z-shaped, and its orthographic projection shape along the direction Z from the card cover 2 to the card seat 1 is rectangular; one end of the spring 41 is fixedly connected to one end of the first head section 11 away from the first middle section 12, and the other end thereof is connected to one side of the Z-shaped slider 422 close to the opening of the first U-shaped structure; the orthographic projection shape of the L-shaped slider 421 along the length direction Y of the first head section 11 is an inverted L shape, and one side of the L-shaped slider 421 away from the Z-shaped slider​​The sliding piece 43 includes a front sliding piece plate 431 and a rear sliding piece plate 432 connected to the front sliding piece plate 431. An L-shaped structure is formed between the front sliding piece plate 431 and the rear sliding piece plate 432. The front sliding piece plate 431 is parallel to the first head segment 11, and the rear sliding piece plate 432 is parallel to the first middle segment 12. One side of the front sliding piece plate 431 close to the first head segment 11 is fixedly connected to one side of the L-shaped slider 421 close to the first tail segment 13. The Z-shaped slider 422 and the L-shaped slider 421 pass through the sliding through hole 212 to drive the sliding piece 43 to slide along the length direction Y of the first head segment 11. A trapezoidal hole is formed in the front sliding piece plate 431. The trapezoidal hole penetrates the front sliding piece plate 431 along the direction X from the first head segment 11 to the first tail segment 13 and extends along the length direction Y of the first head segment 11. A microfluidic chip is elastically clamped in the first trapezoidal groove 131. The trapezoidal hole has a lower bottom surface and an upper bottom surface. Along the direction Z from the clamping cover 2 to the card seat 1, the height of the lower bottom surface is greater than the height of the upper bottom surface, and the lower bottom surface is flush with the rear sliding piece plate 432;

[0043] The rubber strip 3 is arranged parallel to the first middle segment 12. The rubber strip 3 includes fixed ends 31 and movable ends 32 at both ends along its length direction; the fixed ends 31 are fixedly glued to one end of the second tail segment 23 far from the second middle segment 22, and the movable ends 32 are buckled to one side of the rectangular space far from the second middle segment 22.

[0044] Specifically, continue to refer to Figure 2-Figure 4 As shown, the card seat 1 is composed of a first head segment 11, a first middle segment 12 and a first tail segment 13 to form a first C-shaped structure. The opening of the first C-shaped structure of the card seat 1 faces the rubber strip 3. Along the direction Z from the clamping cover 2 to the card seat 1, the orthographic projection of the clamping cover 2 coincides with the orthographic projection of the card seat 11. The card seat 1 includes a first head segment 11, a first middle segment 12 and a first tail segment 13. The first head segment 11, the first middle segment 12 and the first tail segment 13 form a first C-shaped structure with an opening facing the rubber strip 3; the materials of the first head segment 11, the first middle segment 12 and the first tail segment 13 are all rubber. The length extension direction of the first middle segment 12 is the direction X from the first head segment 11 to the first tail segment 13; the direction X from the first head segment 11 to the first tail segment 13 and the direction Z from the clamping cover 2 to the card seat 1 are perpendicular to each other. The two ends of the first middle segment 12 along its length direction are respectively perpendicularly connected to one end of the first head segment 11 and the first tail segment 13. The first head segment 11 and the first tail segment 13 are parallel to each other and have equal lengths along the length direction Y of the first head segment 11. Along the direction X from the first head segment 11 to the first tail segment 13, along the length direction Y of the first head segment 11 and along the direction Z from the clamping cover 2 to the card seat 1, the three are perpendicular to each other in pairs. An L-shaped plate is formed between the first middle segment 12 and the first tail segment 13, that is, the cross-sectional shape perpendicular to the length direction of the first middle segment 12 and the first tail segment 13 is L-shaped. The L-shaped inner right angles of the first middle segment 12 and the first tail segment 13 both face the geometric center line of the card seat 1 along the direction Z from the clamping cover 2 to the card seat 1.

[0045] Further, on one side of the first tail section 13 close to the first head section 11, a first trapezoidal groove 131 recessed away from the first head section 11 is provided. The shape of the positive projection of the first trapezoidal groove 131 along the direction X from the first head section 11 to the first tail section 13 is an isosceles trapezoid, and the cross-sectional shape of the first trapezoidal groove 131 along the direction Z from the card cover 2 to the card seat 1 is several rectangles with different sizes. One side of the first trapezoidal groove 131 corresponding to the lower base of the isosceles trapezoid is coplanar with one side of the first tail section 13 away from the first middle section 12. The first trapezoidal groove 131 can insert the microfluidic chip along the length direction Y of the first head section 11 from the opposite direction of the opening of the first C-shaped structure, and make the side of the microfluidic chip close to the first tail section 13 be clamped in the first trapezoidal groove 131. As the angle of the first trapezoidal groove 131 shrinks, the microfluidic chip can be elastically clamped through the first tail section 13 made of rubber material.

[0046] On one side of the first head section 11 close to the card cover 2, a rectangular lower groove 111 recessed away from the card cover 2 is provided. The length direction of the rectangular lower groove 111 is the length direction Y of the first head section 11; the width of the rectangular lower groove 111 along the direction X from the first head section 11 to the first tail section 13 is smaller than the width of the first head section 11 along the direction X from the first head section 11 to the first tail section 13; the length of the rectangular lower groove 111 along the length direction Y of the first head section 11 is smaller than the length of the first head section 1; the depth of the rectangular lower groove 111 along the direction Z from the card cover 2 to the card seat 1 is smaller than the height of the first head section 11 along the direction Z from the card cover 2 to the card seat 1. A slider 42 and a spring 41 are loaded in the rectangular lower groove 111. Along the direction Z from the card cover 2 to the card seat 1, the cross-sectional shape of the first head section 11 is an inverted F shape; the rectangular opening of the inverted F shape corresponds to the rectangular lower groove 111.

[0047] Continue to refer to Figure 2-Figure 5As shown, the card cover 2 includes a second head section 21, a second middle section 22, and a second tail section 23 that are vertically connected in sequence along its length direction. The second head section 21, the second middle section 22, and the second tail section 23 are all rectangular bars with uniform thickness. The card cover 2 forms a second C-shaped structure by the second head section 21, the second middle section 22, and the second tail section 23. The opening direction of the second C-shaped structure of the card cover 2 is the same as that of the first C-shaped structure, both facing the rubber strip 3. Along the direction Z from the card cover 2 to the card holder 1, the orthographic projection of the second head section 21 coincides with the orthographic projection inside the first head section 11, the orthographic projection of the second middle section 22 coincides within the orthographic projection of the first middle section 12, and the orthographic projection of the second tail section 23 coincides with the orthographic projection of the first tail section 13. On one side of the second head section 21 close to the first head section 11, there is a rectangular upper groove 211 that is recessed away from the first head section 11; the length extension direction of the rectangular upper groove 211 is along the length direction Y of the first head section 11. The openings of the rectangular upper groove 211 and the rectangular lower groove 111 face each other; along the direction Z from the card cover 2 to the card holder 1, the orthographic projection of the rectangular upper groove 211 and the orthographic projection of the rectangular lower groove 111 coincide with each other. After the openings of the rectangular upper groove 211 and the rectangular lower groove 111 are covered with each other, they are fixedly formed into a rectangular space. Optionally, the openings of the rectangular upper groove 211 and the rectangular lower groove 111 can be adhesively covered.

[0048] Further, on one side of the rectangular upper groove 211 and the rectangular lower groove 111 close to the first tail section 13, there is a sliding through hole 212 that penetrates along the direction X from the first head section 11 to the first tail section 13. The sliding through hole 212 is rectangular in shape, and the length extension direction of the sliding through hole 212 is the length direction Y of the first head section 11. The width of the sliding through hole 212 along the direction Z from the card cover 2 to the card holder 1 is less than the sum of the heights of the first head section 11 and the second head section 21 along the direction Z from the card cover 2 to the card holder 1. The function of the sliding through hole 212 is to enable one side of the rectangular space close to the first tail section 13 to communicate with the first C-shaped structure and the second C-shaped structure.

[0049] Continue to refer to Figure 2-Figure 8 As shown, the spring mechanism 4 includes a spring 41, a slider 42, and a sliding plate 43. The spring 41 and the slider 42 are both fixed in the rectangular space along the length direction Y of the first head section 11; and the length directions of the spring 41 and the slider 42 are both the length direction Y of the first head section 11, and the sum of the lengths of the spring 41 and the slider 42 is less than the length of the rectangular space along the length direction Y of the first head section 11; among them, the spring 41 is a metal spring 41, one end of the spring 41 is fixedly connected to one end of the first head section 11 far from the first middle section 12, and the other end of the spring 41 is fixedly connected to the end of the slider 42 far from the first middle section 12. When the slider 42 slides to one end of the rectangular space close to the first middle section 12 and the second middle section 22, the slider 42 can slide through the spring 41 to one end of the rectangular space close to the first head section 11 and the second head section 21.

[0050] The slider 42 is clamped in the rectangular space formed by the rectangular lower groove 111 of the card seat 11 and the rectangular upper groove 211 of the card cover 2, and reciprocates along the length direction Y of the first head section 11 in the rectangular space. The slider 42 includes an L-shaped slider 421 and a Z-shaped slider 422 integrally connected to the L-shaped slider 421 along the length direction Y of the first head section 11.

[0051] Specifically, the Z-shaped slider 422 is a Z-shaped bent rectangular plate, and its material can be plastic or rubber. Along the direction X from the first head section 11 to the first tail section 13, the orthographic projection of the Z-shaped slider 422 is Z-shaped; along the direction Z from the card cover 2 to the card seat 1, the orthographic projection of the Z-shaped slider 422 is rectangular. The height of the Z-shaped slider 422 along the direction Z from the card cover 2 to the card seat 1 is less than the height of the rectangular space along the direction Z from the card cover 2 to the card seat 1. The length extension direction of the Z-shaped slider 422 is the length direction Y of the first head section 11. One end of the Z-shaped slider 422 along its length direction is fixedly connected to the spring 41, and the end of the Z-shaped slider 422 far from the spring 41 is connected to the first middle section 12 through a C-shaped connecting rod 44. The side of the Z-shaped slider 422 close to the first tail section 13 is integrally and equally long connected to the side of the L-shaped slider 421 far from the first tail section 13. The orthographic projection shape of the L-shaped slider 421 along the length direction Y of the first head section 11 is an inverted L shape. The side of the L-shaped slider 42I far from the Z-shaped slider 422 is arranged across the sliding through hole 212. The length of the L-shaped slider 421 along the length direction Y of the first head section 11 is less than the length of the sliding through hole 212 along the length direction Y of the first head section 11; when the Z-shaped slider 422 slides along the length direction Y of the first head section 11 in the rectangular space, a part of the L-shaped slider 421 glued to the Z-shaped slider 422 slides together with the Z-shaped slider 422 along the length direction Y of the first head section 11 in the rectangular space, and the other part of the L-shaped slider 421 slides along the length direction Y in the sliding through hole 212. The side of the L-shaped slider 421 close to the first tail section 13 is equally long glued to the sliding piece l43 in the C-shaped opening of the card seat 1 along the length direction Y of the first head section 11; through the L-shaped slider 421, the Z-shaped slider 422 and the sliding piece 43 maintain the sliding coordination consistency along the length direction Y of the first head section 11.

[0052] The sliding piece 43 is a rectangular piece with an L-shaped bend, and the sliding piece 43 is arranged within the first U-shaped structure of the card holder 1. The sliding piece 43 includes a front sliding piece plate 431 and a rear sliding piece plate 432 perpendicularly connected to the front sliding piece plate 431; an L-shaped structure is formed between the front sliding piece plate 431 and the rear sliding piece plate 432. The front sliding piece plate 431 is parallel to the first head section 11, and the rear sliding piece plate 432 is parallel to the first middle section 12. On the side of the front sliding piece plate 431 close to the first head section 11 and at one end close to the second head section 21, it is fixedly connected to the L-shaped slider 421 along the length direction Y of the first head section 11 to form an L-shaped structure, and the connection method can be adhesive bonding. A trapezoidal hole is formed on the front sliding piece plate 431, and the trapezoidal hole penetrates through the front sliding piece plate 431 along the direction X from the first head section 11 towards the first tail section 13. The length extension direction of the trapezoidal hole is the same as the length direction Y of the first head section 11. The trapezoidal hole has a lower bottom surface and an upper bottom surface. Along the direction Z from the card cover 2 towards the card holder 1, the height of the lower bottom surface is greater than the height of the upper bottom surface, and the area of the lower bottom surface is greater than the area of the upper bottom surface; the lower bottom surface is flush with the rear sliding piece plate 432, and the lower bottom surface and the side of the front sliding piece plate 431 away from the rear sliding piece plate 432 are on the same plane.

[0053] When inserting the microfluidic chip along the length direction Y of the first head section 11, the side of the microfluidic chip close to the first middle section 12 contacts the side of the rear sliding piece plate 432 away from the first middle section 12, and the side of the microfluidic chip close to the first head section 11 is clamped within the trapezoidal hole along the length direction Y of the first head section 11; then insert the microfluidic chip towards the first middle section 12, and the microfluidic chip will push the rear sliding piece plate 432 to slide towards the first middle section 12 together, and at the same time make the L-shaped slider 421 connected to the front sliding piece plate 431 and the Z-shaped slider 422 connected to the L-shaped slider 421 slide towards the first middle section 12 together along the length direction Y of the first head section 11.

[0054] Continue to refer to Figure 2-Figure 9 As shown, the rubber strip 3 is a rectangular strip parallel to the first middle section 12, and its material is rubber with a Shore hardness greater than 50. The rubber strip 3 is parallel to the first middle section 12; along the length direction Y of the first head section 11, the orthographic projections of the first middle section 12 and the second middle section 22 both coincide within the orthographic projection of the rubber strip 3. The rubber strip 3 includes fixed ends 31 and movable ends 32 at both ends in its length extension direction; the fixed ends 31 are fixedly adhesively bonded to one end of the second tail section 23 away from the second middle section 22 and one end of the first tail section 13 away from the first middle section 12. When the microfluidic chip pushes the sliding piece 43 to slide towards the first middle section 12 to the maximum extent, along the length direction Y of the first head section 11, the movable end 32 is movably buckled to the side of the rectangular space away from the first middle section 12 or the second middle section 22.

[0055] In the specific implementation process, this embodiment can be used for the movable clamping of the microfluidic chip. The specific installation operation is as follows:

[0056] First, the microfluidic chip is held along both sides of its width extension direction so that the microfluidic chip is respectively stuck into the first trapezoidal groove 131 away from the end of the first middle section 12 and the trapezoidal hole away from the end of the first middle section 12 along the length direction Y of the first head section 11.

[0057] Then, the microfluidic chip is pushed toward the first middle section 12 along the length direction Y of the first head section 11 , so that the microfluidic chip slides to the maximum extent.

[0058] During the continued sliding process, the microfluidic chip continuously extends along both sides of the first header section 11 in the longitudinal direction Y toward the upper bottom surface of the first trapezoidal groove 131 and the trapezoidal hole, achieving a tighter elastic engagement between the two sides of the microfluidic chip and the first trapezoidal groove 131 and the trapezoidal hole. When the sliding reaches its maximum limit, the spring 41 within the rectangular space is fully extended; at this point, the movable end 32 of the rubber strip 3 is locked into the rectangular space along the longitudinal direction Y of the first header section 11. This completes the loading of the handheld microfluidic chip in this embodiment.

[0059] Furthermore, the side of the card holder 1 of this embodiment away from its card cover 2 is magnetically fixed to the PCR amplification instrument to perform normal sample addition and amplification operations. This step is a conventional operation in this field and will not be described in detail in this embodiment.

[0060] Finally, when the microfluidic chip needs to be disassembled, it is only necessary to pry open the movable end 32 of the rubber strip 3 so that the movable end 32 of the rubber strip 3 is away from the rectangular space; then, along the opposite direction of the length direction Y of the first head section 11, press the side of the microfluidic chip away from the first middle section 12 in the direction of the first middle section 12; the contracted spring 41 drives the Z-shaped slider 422, and the Z-shaped slider 422 drives the L-shaped slider 421 and the slide 43 to make the microfluidic chip pop out in the direction away from the first middle section 12.

[0061] Compared with the prior art, the elastic loading device for a microfluidic chip provided in this embodiment achieves at least the following beneficial effects:

[0062] First, through the elastic loading device for microfluidic chips provided in this embodiment, the microfluidic chip can be quickly and conveniently engaged, which has obvious and significant advantages when testing a large number of samples; first, the card holder 1 provided in this embodiment is provided with a first trapezoidal groove 131, and the card holder 1 is made of rubber, which can elastically engage the microfluidic chip on one side along its length direction; further, the slide 43 provided in this embodiment has a trapezoidal hole, which can elastically engage the microfluidic chip on the other side along its length direction; this engaging is automatically completed during the loading process of the microfluidic chip, and can be achieved without additional fixing, which facilitates the rapid loading of a large number of chips; in addition, the rubber strip 3 provided in this embodiment can complete the sealing operation by buckling after the microfluidic chip is loaded, which further saves loading time.

[0063] Second, the elastic loading device for the microfluidic chip provided in this embodiment can stably support the chip, thereby reducing the operational risks during the detection process; first, the first trapezoidal groove 131 and the trapezoidal hole provided in this embodiment directly support and fix the microfluidic chip from both sides along its width direction, making the fixation of the microfluidic chip more stable; secondly, the slide back plate 432 and the rubber strip 3 provided in this embodiment clamp and fix the microfluidic chip from both sides along its length direction. Combined with the first trapezoidal groove 131 and the trapezoidal hole, the four sides of the microfluidic chip along its circumferential direction are fixed; a firmly fixed microfluidic chip can reduce the operational risks during the detection process; in addition, the loading and disassembly of the microfluidic chip only requires contact with the side of the microfluidic chip along its circumferential direction, which can reduce the risk of contamination on the chip surface.

[0064] In an alternative embodiment, continue to refer to Figure 2 ,and Figure 6-Figure 8 As shown, the Z-shaped slider 422 includes a Z-shaped upper portion 4223, a Z-shaped middle portion 4222, and a Z-shaped lower portion 4221 that are vertically connected in sequence. The Z-shaped upper portion 4223, the Z-shaped middle portion 4222, and the Z-shaped lower portion 4221 are all rectangular plates with the same width X along the direction X from the first head section 11 to the first tail section 13. The length extension directions of the Z-shaped upper portion 4223 and the Z-shaped lower portion 4221 are respectively the same as the length extension direction of the L-shaped slider 421. The Z-shaped lower portion 4221 is fixedly connected to the spring 41, and the length extension direction of the Z-shaped middle portion 4222 is perpendicular to the length extension direction of the L-shaped slider 421.

[0065] A sliding groove 423 is formed on one side of the Z-shaped upper portion 4223 near the second head section 21, which is recessed away from the second head section 21. The sliding groove 423 includes a first sliding in groove 4231, a second sliding in groove 4232, a first sliding out groove 4233, a second sliding out groove 4234, and a third sliding out groove 4235. The first sliding in groove 4231, the second sliding in groove 4232, the first sliding out groove 4233, and the third sliding out groove 4235 are rectangular grooves with different groove depths.

[0066] The first sliding in slot 4231 and the second sliding out slot 4234 are parallel and are respectively arranged at both ends of the Z-shaped upper portion 4223 along the direction X from the first head section 11 to the first tail section 13. The first sliding in slot 4231 and the second sliding out slot 4234 are flush with each other along the direction X from the first head section 11 to the first tail section 13 at one end of the Z-shaped middle portion 4222. Along the length direction Y of the first head section 11, the length of the second sliding out slot 4234 is shorter than the length of the first sliding in slot 4231.

[0067] The lengths of the second sliding-in groove 4232 and the first sliding-out groove 4233 are equal. One end of the second sliding-in groove 4232 and one end of the first sliding-out groove 4233 form a first obtuse angle α, and the angle of the first obtuse angle α faces the side of the Z-shaped middle part 4222; the other end of the second sliding-in groove 4232 and the end of the first sliding-in groove 4231 far from one end of the first middle segment 12 form a first acute angle β; the other end of the first sliding-out groove 4233 and the end of the second sliding-out groove 4234 far from one end of the first middle segment 12 form a second acute angle γ; the sum of the first acute angle β and the second acute angle γ is equal to the first obtuse angle α.

[0068] One end of the second sliding-out groove 4234 close to the first middle segment 12 and one end of the third sliding-out groove 4235 form a second obtuse angle δ, and the other end of the third sliding-out groove 4235 and the side of the first sliding-in groove 4231 far from the L-shaped slider 421 form a third acute angle θ; the second obtuse angle δ and the third acute angle θ are a pair of complementary angles; along the direction X from the first head segment 11 to the first tail segment 13, the orthographic projection lengths of the second sliding-out groove 4234 and the third sliding-out groove 4235 are not greater than the orthographic projection length of the first sliding-in groove 4231.

[0069] The spring mechanism 4 further includes a U-shaped connecting rod 44. A cylindrical groove 114 recessed in the direction away from the second middle segment 22 is provided on the side of the first middle segment 12 close to the first head segment 11 and close to the Z-shaped upper part 4223; one end of the U-shaped connecting rod 44 is inserted into the cylindrical groove 114, and the other end is inserted into the first sliding-in groove 4231, the second sliding-in groove 4232, the first sliding-out groove 4233, the second sliding-out groove 4234 or the third sliding-out groove 4235.

[0070] Specifically, the Z-shaped slider 422 includes a Z-shaped upper part 4223, a Z-shaped middle part 4222 and a Z-shaped lower part 4221 that are sequentially and perpendicularly connected. The Z-shaped upper part 4223, the Z-shaped middle part 4222 and the Z-shaped lower part 4221 are all rectangular plates with the same width along the direction X from the first head segment 11 to the first tail segment 13; the widths of the Z-shaped upper part 4223, the Z-shaped middle part 4222 and the Z-shaped lower part 4221 along the direction X from the first head segment 11 to the first tail segment 13 are smaller than the width of the rectangular space along the direction X from the first head segment 11 to the first tail segment 13. Among them,

[0071] A sliding groove 423 is defined on one side of the Z-shaped upper portion 4223, proximal to the second head section 21, and recessed away from the second head section 21. The sliding groove 423 is a linear groove comprising a first inlet groove 4231, a second inlet groove 4232, a first outlet groove 4233, a second outlet groove 4234, and a third outlet groove 4235, which are connected and connected to form an annular structure. Along the direction Z pointing from the card cover 2 to the card seat 1, the orthographic projections of the first inlet groove 4231, the second inlet groove 4232, the first outlet groove 4233, the second outlet groove 4234, and the third outlet groove 4235 all overlap within the orthographic projection of the Z-shaped upper portion 4223. The first sliding-in groove 4231, the second sliding-in groove 4232, the first sliding-out groove 4233, the second sliding-out groove 4234 and the third sliding-out groove 4235 are rectangular grooves with different groove depths; wherein, along the direction Z pointing from the card cover 2 to the card seat 1, the groove depth of the first sliding-in groove 4231 is greater than the groove depth of the third sliding-out groove 4235, the groove depth of the second sliding-in groove 4232 is greater than the groove depth of the first sliding-in groove 4231, and the groove bottom of the first sliding-out groove 4233 is an inclined groove for connecting the groove bottom of the second sliding-in groove 4232 and the groove bottom of the second sliding-out groove 4234; the groove depth of the second sliding-out groove 4234 and the third sliding-out groove 4235 is the same.

[0072] Specifically, a cylindrical groove 114 is defined on the side of the first middle section 12 near the first head section 11 and near the Z-shaped upper portion 4223, extending further away from the second middle section 22. A U-shaped connecting rod 44 is inserted into this cylindrical groove 114 along one end of its length. The U-shaped connecting rod 44 is a round rod bent in a U-shape and made of metal. The opening of the U-shaped connecting rod 44 is oriented in the same direction as the rectangular upper groove 211.

[0073] The first sliding-in groove 4231 and the second sliding-out groove 4234 are parallel to each other and have different lengths. Along the length direction Y of the first head section 11, the length of the second sliding-out groove 4234 is less than the length of the first sliding-in groove 4231. The first sliding-in groove 4231 and the second sliding-out groove 4234 are respectively arranged at both ends of the Z-shaped upper portion 4223 along the direction X from the first head section 11 to the first tail section 13. The first sliding-in groove 4231 and the second sliding-out groove 4234 are flush near one end of the Z-shaped middle portion 4222, and along the direction X from the first head section 11 to the first tail section 13, the orthographic projection of the second sliding-out groove 4234 partially overlaps with the orthographic projection of the first sliding-in groove 4231.

[0074] The second sliding-in groove 4232 and the first sliding-out groove 4233 are arranged between the first sliding-in groove 4231 and the second sliding-out groove 4234. The second sliding-in groove 4232 and the first sliding-out groove 4233 are equal in length, and the extending direction of their lengths is mirror-symmetrical along the direction X from the first head segment 11 to the first tail segment 13. One end of the second sliding-in groove 4232 penetrates through the side far from the L-shaped slider 421 at an acute angle β with the end of the first sliding-in groove 4231 far from the first middle segment 12. The groove depth of the second sliding-in groove 4232 is greater than that of the first sliding-in groove 4231; when one end of the U-shaped connecting rod 44 slides along the first sliding-in groove 4231 to near the penetration point with the second sliding-in groove 4232, the groove depth of the second sliding-in groove 4232 is greater than that of the first sliding-in groove 4231, and one end of the U-shaped connecting rod 44 automatically turns and enters the second sliding-in groove 4232. The other end of the second sliding-in groove 4232 along its length is connected and penetrates through one end of the first sliding-out groove 4233 along its length at an obtuse angle α, and the obtuse angle α faces the side of the Z-shaped middle part 4222. When the spring 41 contracts along the length direction Y of the first head segment 11, one end of the U-shaped connecting rod 44 can be clamped in the obtuse angle α between the second sliding-in groove 4232 and the first sliding-out groove 4233. The end of the first sliding-out groove 4233 far from the second sliding-in groove 4232 penetrates through the side near the L-shaped slider 421 at an acute angle γ with the end of the second sliding-out groove 4234 far from the first middle segment 12. The second sliding-in groove 4232 and the first sliding-out groove 4233 connect and penetrate through the mutually parallel first sliding-in groove 4231 and the second sliding-out groove 4234, and the sum of the acute angle β and the acute angle γ is equal to the obtuse angle α. When disassembling the microfluidic chip, pressing the microfluidic chip towards the first middle segment 12, due to the groove depth of the second sliding-in groove 4232 being greater than that of the first sliding-in groove 4231, one end of the U-shaped connecting rod 44 originally clamped in the obtuse angle α cannot return to the first sliding-in groove 4231 because of the groove depth difference, so that the U-shaped connecting rod 44 continues to slide into the second sliding-out groove 4234.

[0075] One end of the second sliding-out groove 4234 near the first middle segment 12 is connected and penetrates through one end of the third sliding-out groove 4235 at an obtuse angle δ. The U-shaped connecting rod 44 sliding into the second sliding-out groove 4234 continues to slide into the third sliding-out groove 4235. The end of the third sliding-out groove 4235 far from the second sliding-out groove 4234 penetrates through the side far from the L-shaped slider 421 of the first sliding-in groove 4231 at an acute angle θ. Through the third sliding-out groove 4235, one end of the U-shaped connecting rod 44 finally returns to the first sliding-in groove 4231. The first sliding-in groove 4231 and the second sliding-out groove 4234 are mutually parallel, and the obtuse angle δ and the acute angle θ are a pair of complementary angles. Along the direction X from the first head segment 11 to the first tail segment 13, the orthographic projection lengths of the second sliding-out groove 4234 and the third sliding-out groove 4235 are not greater than the orthographic projection length of the first sliding-in groove 4231.

[0076] Through the above solution, one end of the C-shaped connecting rod 44 can continuously slide along the fixed direction in the first sliding-in groove 4231, the second sliding-in groove 4232, the first sliding-out groove 4233, the second sliding-out groove 4234 and the third sliding-out groove 4235 of the annular structure. By the different positions of the C-shaped connecting rod 44 in the first sliding-in groove 4231, the second sliding-in groove 4232, the first sliding-out groove 4233, the second sliding-out groove 4234 and the third sliding-out groove 4235, the spring 41 is kept in a contracted or expanded state; when one end of the C-shaped connecting rod 44 is fixed in the first obtuse angle α between the second sliding-in groove 4232 and the first sliding-out groove 4233, the C-shaped connecting rod 44 hooks the Z-shaped upper part 4223 towards the first middle section 12, making it impossible for the spring 41 to contract, so that the microfluidic chip is fixed in the opening of the first C-shaped structure of the card holder 1; when one end of the C-shaped connecting rod 44 slides out of the first obtuse angle α, the spring 41 can contract, and the microfluidic chip pops out along the length direction Y of the first head section 11.

[0077] In an alternative embodiment, continue to refer to Figure 2 、 Figure 6 、 Figure 7 、 Figure 8 and Figure 10 as shown in Figure 10 is Figure 2 the enlarged view of part A in

[0078] In this embodiment, a first fixing cylinder 112 extending along the direction Z from the card cover 2 to the card holder 1 is fixed at the bottom of the end of the rectangular lower groove 111 far from the first middle section 12. The length of the first fixing cylinder 112 along the direction Z from the card cover 2 to the card holder 1 is not greater than the height of the rectangular space. One end of the spring 41 is sleeved on the outer wall of the first fixing cylinder 112;

[0079] An arc-shaped through hole 424 penetrating the Z-shaped lower part 4221 along the direction Z from the card cover 2 to the card holder 1 is provided in the Z-shaped lower part 4221. The arc-shaped through hole 424 is recessed towards the first middle section 12; along the direction Z from the card cover 2 to the card holder 1, the orthographic projection of the arc-shaped through hole 424, the orthographic projection of the Z-shaped middle partSpecifically, an arcuate through hole 424 is formed at one end of the Z-shaped lower portion 4221 away from the Z-shaped middle portion 4222, extending through the Z-shaped lower portion 4221 along the direction Z from the card cover 2 to the card seat 1. The arcuate through hole 424 is a semicircular through hole, and the arcuate recess of the arcuate through hole 424 is directed toward the first middle section 12. Along the direction Z from the card cover 2 to the card seat 1, the orthographic projections of the arcuate through hole 424, the Z-shaped middle portion 4222, and the Z-shaped upper portion 4223 do not overlap, and the shortest distance from the arcuate through hole 424 to the Z-shaped middle portion 4222 is greater than the diameter of the spring wire of the spring 41. A second fixed cylinder 425 extending along the direction Z from the card cover 2 to the card seat 1 is glued into the arcuate through hole 424. The second fixed cylinder 425 is a cylinder of uniform thickness; the first fixed cylinder 112 and the second fixed cylinder 425 have the same structure; the material of the second fixed cylinder 425 can be plastic or rubber. The length of the second fixing cylinder 425, along the direction Z pointing from the card cover 2 to the card seat 1, is no greater than the height of the rectangular space. The end of the second fixing cylinder 425, which is closest to the rectangular lower groove 111, is glued to the Z-shaped lower portion 4221 via the arcuate through-hole 424. The curvature of the outer surface of the second fixing cylinder 425 is the same as the curvature of the arcuate through-hole 424. The outer surface of the second fixing cylinder 425 also sleeves onto one end of the spring 41, pulling the end of the spring 41, which is closest to the first middle section 12, to reciprocate along the length direction Y of the first head section 11.

[0080] A first fixed cylinder 112 extending in the direction Z from the card cover 2 to the card seat 1 is fixed to the bottom of the rectangular lower groove 111 at one end away from the first middle section 12. The first fixed cylinder 112 and the second fixed cylinder 425 are of equal size; the first fixed cylinder 112 is a cylinder with uniform thickness; its material can be plastic or rubber. The orthographic projections of the first fixed cylinder 112 and the second fixed cylinder 425 along the length direction Y of the first head section 11 coincide with each other. The female surface of the first fixed cylinder 112 is sleeved on the end of the fixing spring 41 away from the second fixed cylinder 425. When the female surface of the second fixed cylinder 425 is sleeved on one end of the spring 41 and the spring 41 is pulled along the length direction Y of the first head section 11 to stretch close to one end of the first middle section 12, the first fixed cylinder 112 can keep the other end of the spring 41 fixed.

[0081] Through the above solution, the second fixing cylinder 425 and the arc-shaped through hole 424 can achieve the connection between the spring 41 and the Z-shaped lower portion 4221, and enable this end of the spring 41 to slide together with the Z-shaped lower portion 4221. The first fixing cylinder 112 can achieve a fixed connection between the other end of the spring 41 and the first head section 11, so that this end of the spring 41 can be fixed to the first head section 11.

[0082] In an alternative embodiment, continue to refer to Figure 2-Figure 8As shown, the L-shaped slider 421 includes a horizontal section 4211 and a vertical section 4212 connected to the horizontal section 4211. An inverted L-shaped structure is formed between the horizontal section 4211 and the vertical section 4212. The Z-shaped upper part 4223, the Z-shaped middle part 4222 and the Z-shaped lower part 4221 are bonded to the vertical section 4212 in sequence along the length direction Y of the first head section 11. The horizontal section 4211 is inserted into the sliding through hole 212 and fixedly connected to the sliding front plate 431.

[0083] Specifically, the horizontal section 4211 and the vertical section 4212 are perpendicularly connected to each other to form an L-shaped slider 421, and the horizontal section 4211 and the vertical section 4212 are integrally connected along the length direction Y of the first header section 11. The horizontal section 4211 and the vertical section 4212 form an inverted L-shaped structure, that is, the orthographic projection of the horizontal section 4211 and the vertical section 4212 along the length direction Y of the first header section is an inverted L-shape. The Z-shaped upper portion 4223, the Z-shaped middle portion 4222, and the Z-shaped lower portion 4221 are sequentially bonded to the vertical section 4212 along the length direction Y of the first header section 11. The sum of the lengths of the Z-shaped upper portion 4223, the Z-shaped middle portion 4222, and the Z-shaped lower portion 4221 along the length direction Y of the first header section 11 is equal to the length of the vertical section 4212 along the length direction Y of the first header section 11. Among them, the side of the Z-shaped upper part 4223 close to the second head section 21 and the side of the vertical section 4212 close to the second head section 21 are in the same plane, and the side of the Z-shaped lower part 4221 away from the second head section 21 and the side of the vertical section 4212 away from the second head section 21 are in the same plane.

[0084] The horizontal section 4211 passes through the sliding hole 212 along the direction X from the first leading section 11 to the first trailing section 13. The length of the horizontal section 4211 along the length direction Y of the first leading section 11 is less than the length of the sliding hole 212 along the length direction Y of the first leading section 11. The horizontal section 4211 is inserted into the sliding hole 212. The side of the horizontal section 4211 away from the first trailing section 13 is perpendicularly connected to the side of the vertical section 4212 near the first trailing section 13. The side of the horizontal section 4211 away from the vertical section 4212 is fixedly connected to the slide front plate 431. The horizontal section 4211, the vertical section 4212, and the side of the slide front plate 431 near the second leading section 21 are coplanar.

[0085] Through the above scheme, the horizontal section 4211 can be inserted into the sliding through hole 212 along the length direction Y of the first head section 11, so that the L-shaped slider 421 can be fixedly connected to the slide front plate 431, and the vertical section 4212 can be simultaneously connected to the Z-shaped upper part 4223, the Z-shaped middle part 4222 and the Z-shaped lower part 4221 along the length direction Y of the first head section 11. The horizontal section 4211 and the vertical section 4212 connect the slide front plate 431 and the Z-shaped upper part 4223, the Z-shaped middle part 4222 and the Z-shaped lower part 4221 inside and outside the rectangular space of the first head section 11, so that the slide front plate 431 can drive the Z-shaped upper part 4223, the Z-shaped middle part 4222 and the Z-shaped lower part 4221 to slide along the length direction Y of the first head section 11.

[0086] In an alternative embodiment, continue to refer to Figure 2-Figure 4 As shown, a second trapezoidal groove extending along the length direction Y of the first head section 11 is provided on the side of the first tail section 13 of the first head section 11; the second trapezoidal groove is recessed in the direction away from the first trapezoidal groove 131, and is directed along the direction X of the first head section 11 pointing to the first tail section 13. The second trapezoidal groove is not connected to the rectangular lower groove 111, and the end of the second trapezoidal groove away from the first middle section 12 passes through the first head section 11; along the direction X of the first head section 11 pointing to the first tail section 13, the orthographic projection of the first trapezoidal groove 131 and the orthographic projection of the second trapezoidal groove coincide with each other.

[0087] Specifically, a second trapezoidal groove extending along the length direction Y of the first head section 11 is provided on the side of the first tail section 13 of the first head section 11; the second trapezoidal groove is recessed in the direction away from the first trapezoidal groove 131. Along the direction X from the first head section 11 to the first tail section 13, the second trapezoidal groove and the rectangular lower groove 111 do not penetrate the first head section 11; along the length direction Y of the first head section 11, the end of the second trapezoidal groove away from the first middle section 12 penetrates the side of the first head section 11 away from the first middle section 12. The orthographic projection of the second trapezoidal groove along the direction X from the first head section 11 to the first tail section 13 is a trapezoid. Along the direction X from the first head section 11 to the first tail section 13, the orthographic projection of the first trapezoidal groove 131 and the orthographic projection of the second trapezoidal groove coincide with each other.

[0088] With the above solution, after the microfluidic chip is respectively engaged in the first trapezoidal groove 131 and the trapezoidal hole on both sides along the length direction Y of the first head section 11, the side of the microfluidic chip closer to the trapezoidal hole can be further engaged in the second trapezoidal groove. This can increase the engagement stability of the side of the microfluidic chip closer to the trapezoidal hole.

[0089] In an alternative embodiment, continue to refer to Figure 2 、 Figure 3 、 Figure 9 、 Figure 10 and Figure 11 As shown, Figure 111 is a partial structural diagram of the first head section, the first fixed cylinder, and the triangular prism provided by the present invention; a triangular prism 113 extending in the direction Z from the card cover 2 to the card seat 1 is glued to the end of the first head section 11 away from the first middle section 12, and two triangular prisms 113 are provided, one triangular prism 113 is fixedly glued along one side of its length to the side of the first head section 11 close to the first tail section 13, and the other triangular prism 113 is fixedly glued along one side of its length to the side of the first head section 11 away from the first tail section 13; along the length direction Y of the first head section 11, the orthographic projection of the triangular prism 113 completely overlaps with the orthographic projection of the rectangular lower groove 111;

[0090] A rubber boss 321 is glued to the side of the movable end 32 near the rectangular lower groove 111; along the length direction Y of the first head section 11, the orthographic projection of the boss 321 coincides with the orthographic projection of the movable end 32; the width of the boss 321 along the direction X from the first head section 11 to the first tail section 13 is greater than the distance between the two triangular prisms 113, and the boss 321 is buckled in the rectangular space corresponding to the two triangular prisms 113.

[0091] Specifically, a triangular prism 113 extending in the direction Z from the card cover 2 to the card base 1 is glued to one end of the rectangular lower groove 111 away from the first middle section 12. The triangular prism 113 is made of plastic. Two triangular prisms 113 are glued together, and the two triangular prisms 113 are mirror-symmetrical about the central axis of the rectangular lower groove 111 along the length direction Y of the first head section 11. One triangular prism 113 is glued to the side of the first head section 11 near the first tail section 13 along one side of its length, and the other triangular prism 113 is glued to the side of the first head section 11 away from the first tail section 13 along one side of its length. The other side of the two triangular prisms 113 along their length overlaps with the side of the rectangular lower groove 111 away from the first middle section 12. Along the length direction Y of the first head section 11, the orthographic projection of the triangular prism 113 completely overlaps with the orthographic projection of the rectangular lower groove 11. The two triangular prisms 113 are used to close the opening of the rectangular lower groove 111 away from the first middle section 12 .

[0092] A rubber boss 321 is glued to one side of the movable end 32 near the rectangular lower groove 111; the boss 321 is a rectangular block. The length of the boss 321 along the direction X from the first head section 11 to the first tail section 13 is less than the length of the rubber strip 3; the width of the boss 321 along the direction Z from the card cover 2 to the card seat 1 is less than the width of the rubber strip 3. Along the length direction Y of the first head section 11, the orthographic projection of the boss 321 coincides with the orthographic projection of the movable end 32. Furthermore, the width of the boss 321 along the direction X from the first head section 11 to the first tail section 13 is greater than the distance between the two triangular prisms 113, and the boss 321 can be buckled into the rectangular space through the two triangular prisms 113 along the opposite direction of the length direction Y of the first head section 11.

[0093] Through the above solution, the elastic fastening between the rubber strip 3 and the card seat 1 can be achieved, specifically through the boss body 321 at the movable end 32 of the rubber strip 3, the triangular prism 113 and the rectangular lower groove 111 on the first head section 11. Through the elasticity of the boss body 321, the rubber strip 3 can seal the card seat 1.

[0094] Embodiment 2

[0095] This embodiment is an extended solution based on Embodiment 1. Continue to refer to Figure 2 、 Figure 3 、 Figure 4 and Figure 12 as shown. Figure 12 is Figure 3 the enlarged view at B in ; A fixing piece 5 made of a magnetic metal material is adhesively bonded to the side of the card seat 1 away from the card cover 2. The fixing piece 5 is a third C-shaped structure, and the opening of the third C-shaped structure is the same as the opening of the first C-shaped structure. Along the direction Z from the card cover 2 to the card seat 1, the orthographic projection of the third C-shaped structure and the orthographic projection of the first C-shaped structure coincide with each other.

[0096] Specifically, the fixing piece 5 is a metal sheet made of a magnetic material, and it is specifically arranged on the side of the card seat 1 away from the card cover 2; the fixing piece 5 is adhesively bonded to completely coincide with the first head section 11, the first middle section 12 and the first tail section 13 of the card seat 1. The fixing piece 5 is a third C-shaped structure identical to the first C-shaped structure or the second C-shaped structure 2, and the orientation of the third C-shaped structure of the fixing piece 5 is the same as the orientation of the first C-shaped structure of the card seat 1 or the second C-shaped structure of the card cover 2. Along the direction Z from the card cover 2 to the card seat 1, the orthographic projection of the fixing piece 5 and the orthographic projection of the card seat 1 coincide with each other; the orthographic projection of the third C-shaped structure and the orthographic projection of the first C-shaped structure coincide with each other. The thickness of the fixing piece 5 along the direction Z from the card cover 2 to the card seat 1, the thickness of the card seat 1, and the thickness of the card cover 2 along the direction Z from the card cover 2 to the card seat 1 add up to the width of the rubber sheet along the direction Z from the card cover 2 to the card seat 1.

[0097] Through the above solution, the card seat 1, the rubber sheet, the spring mechanism 4 and the card cover 2 can be integrally magnetically fixed in the PCR amplifier through the fixing piece 5; it is convenient to install a kind of elastic loading device for the microfluidic chip provided in this embodiment. Further, the size of the fixing piece 5 completely coincides with the first head section 11, the first middle section 12 and the first tail section 13, so that the magnetic adsorption area can be maximally expanded, thereby improving the magnetic fixing stability of the elastic loading device for the microfluidic chip in the PCR amplifier in this embodiment.

[0098] There is a relationship of mutual coordination and functional support between the various technical features of the present invention, that is, the technical solution is not a "simple superposition" of technical features between multiple comparative documents, so the present invention does not fall under the "simple superposition" situation in Chapter 4 of Part 2 of the "Patent Examination Guidelines".

[0099] Although some specific embodiments of the present invention have been described in detail by way of examples, it should be understood by those skilled in the art that the above examples are for illustration only and are not intended to limit the scope of the present invention. It should be understood by those skilled in the art that modifications may be made to the above embodiments without departing from the scope and spirit of the present invention. The scope of the present invention is defined by the appended claims.

Claims

1. An elastic loading device for a microfluidic chip, characterized in that: It includes a card seat, a card cover, a spring mechanism and a rubber strip; wherein, The card base is covered with the card cover, which includes a first head section, a first middle section, and a first tail section connected in sequence, and a first U-shaped structure is formed between the first head section, the first middle section, and the first tail section; a first trapezoidal groove is formed on a side of the first tail section close to the first head section, which is recessed away from the first head section, and the first trapezoidal groove extends along the length direction of the first head section; a rectangular lower groove is formed on a side of the first head section close to the card cover, which is recessed away from the card cover, and the rectangular lower groove extends along the length direction of the first head section; along the direction from the card cover to the card base, the cross-section of the first head section is an inverted F shape; and a second end of the second end of the second end is connected to the support frame, wherein the second end of the second end is connected to the support frame by the support leg and the support leg is connected to the support leg. The spring mechanism includes a slider, a spring and a sliding plate, and the slider is clamped in the rectangular space; the slider includes an L-shaped slider and a Z-shaped slider integrally connected to the L-shaped slider; one end of the L-shaped slider and the Z-shaped slider faces the opening of the first �-shaped structure, and the other end faces away from the opening of the first �-shaped structure; the orthographic projection shape of the Z-shaped slider along the direction of the first head section pointing to the first tail section is Z-shaped, and the orthographic projection shape of the direction of the card cover pointing to the card seat is rectangular; one end of the spring is fixedly connected to the end of the first head section away from the first middle section, and the other end is connected to the side of the Z-shaped slider close to the opening of the first �-shaped structure; the orthographic projection shape of the L-shaped slider along the length direction of the first head section is an inverted L-shape, and the side of the L-shaped slider away from the Z-shaped slider is arranged to cross the sliding through hole; The sliding plate comprises a sliding plate front plate and a sliding plate rear plate connected to the sliding plate front plate, the sliding plate and the sliding plate rear plate forming an L-shaped structure, the sliding plate front plate is parallel to the first head section, the sliding plate rear plate is parallel to the first middle section, the sliding plate front plate is fixedly connected to a side of the L-shaped slider close to the first tail section through the sliding through hole, the Z-shaped slider and the L-shaped slider drive the slider to slide along the length direction of the first head section, the sliding plate front plate is provided with a trapezoidal hole, the trapezoidal hole passes through the sliding front plate in the direction of the first head section pointing to the first tail section, and extends along the length direction of the first head section, the microfluidic chip is elastically snapped into between the first trapezoidal groove and the trapezoidal hole, the trapezoidal hole has a lower bottom surface and an upper bottom surface, and along the direction of the card cover pointing to the card seat, the height of the lower bottom surface is greater than the height of the upper bottom surface, and the lower bottom surface is flush with the sliding rear plate; The rubber strip is arranged parallel to the first middle section, and includes a fixed end and a movable end at both ends along its length direction; the fixed end is fixedly glued to one end of the second tail section away from the second middle section, and the movable end is buckled to one side of the rectangular space away from the second middle section.

2. The elastic loading device for a microfluidic chip according to claim 1, characterized in that: The Z-shaped slider includes a Z-shaped upper portion, a Z-shaped middle portion, and a Z-shaped lower portion that are vertically connected in sequence, and the Z-shaped upper portion, the Z-shaped middle portion, and the Z-shaped lower portion are all rectangular plates with the same width along the direction from the first head section to the first tail section; the length extension directions of the Z-shaped upper portion and the Z-shaped lower portion are respectively the same as the length extension direction of the L-shaped slider; the Z-shaped lower portion is fixedly connected to the spring, and the length extension direction of the Z-shaped middle portion is perpendicular to the length extension direction of the L-shaped slider; A sliding groove is provided on the side of the Z-shaped upper portion close to the second head section, which is recessed away from the second head section. The sliding groove includes a first sliding in groove, a second sliding in groove, a first sliding out groove, a second sliding out groove and a third sliding out groove that are connected to each other; the first sliding in groove, the second sliding in groove, the first sliding out groove and the third sliding out groove are respectively rectangular grooves with different groove depths; The first sliding in slot and the second sliding out slot are parallel and are respectively arranged at both ends of the Z-shaped upper portion in a direction from the first leading section to the first trailing section. The first sliding in slot and the second sliding out slot at one end close to the Z-shaped middle portion are flush along a direction from the first leading section to the first trailing section. Along the length direction of the first leading section, the length of the second sliding out slot is shorter than the length of the first sliding in slot. The second sliding in groove and the first sliding out groove are of equal length; one end of the second sliding in groove forms a first obtuse angle with one end of the first sliding out groove, with the angle of the first obtuse angle facing the middle of the Z-shaped portion; the other end of the second sliding in groove forms a first acute angle with an end of the first sliding in groove away from the first middle section; the other end of the first sliding out groove forms a second acute angle with an end of the second sliding out groove away from the first middle section; the sum of the first acute angle and the second acute angle is equal to the first obtuse angle; One end of the second sliding groove close to the first middle section forms a second obtuse angle with one end of the third sliding groove, and the other end of the third sliding groove forms a third acute angle with the side of the first sliding groove away from the L-shaped slider; the second obtuse angle and the third acute angle are a pair of complementary angles; along the direction from the first head section to the first tail section, the orthographic projection lengths of the second sliding groove and the third sliding groove are not greater than the orthographic projection length of the first sliding groove; The spring mechanism further includes a U-shaped connecting rod. A cylindrical groove recessed away from the second middle section is formed on one side of the first middle section close to the first head section and close to the upper part of the Z shape; one end of the U-shaped connecting rod is inserted into the cylindrical groove, and the other end thereof is inserted into the first sliding groove, the second sliding groove, the first sliding-out groove, the second sliding-out groove or the third sliding-out groove.

3. The elastic loading device for a microfluidic chip according to claim 2, characterized in that: One end of the rectangular lower groove away from the first middle section is fixed with a first fixed cylinder extending in the direction from the card cover to the card seat. The length of the first fixed cylinder in the direction from the card cover to the card seat is not greater than the height of the rectangular space. One end of the spring is sleeved on the outer wall of the first fixed cylinder; The lower part of the Z shape is provided with an arc-shaped through hole penetrating through the lower part of the Z shape in the direction from the card cover to the card seat, and the arc-shaped through hole is recessed towards the first middle section; along the direction from the card cover to the card seat, the orthographic projections of the arc-shaped through hole, the middle part of the Z shape and the upper part of the Z shape do not overlap each other; a second fixed cylinder extending in the direction from the card cover to the card seat is glued in the arc-shaped through hole, and the first fixed cylinder is parallel to the second fixed cylinder; the other end of the spring is sleeved on the outer wall of the second fixed cylinder, and the lower part of the Z shape pulls the other end of the spring to reciprocate and stretch along the length direction of the first head section.

4. The elastic loading device for a microfluidic chip according to claim 2, characterized in that: The L-shaped slider includes a horizontal section and a vertical section connected to the horizontal section. An inverted L-shaped structure is formed between the horizontal section and the vertical section. The upper part of the Z shape, the middle part of the Z shape and the lower part of the Z shape are adhesively connected to the vertical section in sequence along the length direction of the first head section, and the horizontal section is inserted into the sliding through hole and fixedly connected to the sliding plate front plate.

5. The elastic loading device for a microfluidic chip according to claim 1, characterized in that: A second trapezoidal groove extending along the length direction of the first head section is formed on one side of the first head section close to the first tail section; the second trapezoidal groove is recessed away from the first trapezoidal groove. Along the direction from the first head section to the first tail section, the second trapezoidal groove is not communicated with the rectangular lower groove, and one end of the second trapezoidal groove away from the first middle section penetrates through the first head section; along the direction from the first head section to the first tail section, the orthographic projections of the first trapezoidal groove and the second trapezoidal groove coincide with each other.

6. The elastic loading device for a microfluidic chip according to claim 1, characterized in that: A triangular prism extending in the direction from the card cover to the card seat is glued to one end of the first head section away from the first middle section. Two triangular prisms are provided. One triangular prism is fixedly glued to a side of the first head section close to the first tail section along one side of its length, and the other triangular prism is fixedly glued to a side of the first head section away from the first tail section along one side of its length. Along the length direction of the first head section, the orthographic projection of the triangular prism completely overlaps with the orthographic projection of the rectangular lower groove. A boss body is glued to the side of the movable end close to the rectangular lower groove; along the length direction of the first head section, the orthographic projection of the boss body coincides with the orthographic projection of the movable end; the width of the boss body along the direction from the first head section to the first tail section is greater than the distance between the two triangular prisms, and the boss body is buckled in the rectangular space corresponding to the two triangular prisms.

7. The elastic loading device for a microfluidic chip according to claim 6, characterized in that: The material of the boss body is rubber.

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