A tea pesticide residue detection sample preparation device and a preparation method thereof

Abstract

The application discloses a kind of tea agricultural residues detection with sample preparation device and preparation method for measured sample, comprising: machine table, three-axis gantry moving frame, feeding structure, oscillation table, pipe body transfer structure, sample transfer frame, further comprising: multifunctional linkage structure, oscillation table contains main oscillation area and the several vice oscillation areas of being separately arranged in the periphery of main oscillation area, multifunctional linkage structure contains the main drive part being arranged in the lower side of main oscillation area, the lower end of vice oscillation area is movably installed with vice drive part, vice drive part is connected with vice oscillation area by a connecting piece, and the side of vice oscillation area close to main oscillation area is connected with main oscillation area by linkage piece;Lower separation structure contains the separation piece being arranged in the lower end of main oscillation area, and the lower end of separation piece is provided with a lower drive part, and the application can improve the full-automatic processing efficiency of sample, to improve the efficiency of back-end detection.

Description

TECHNICAL FIELD

[0001] The present application relates to tea detection, in particular to a tea pesticide residue detection sample preparation device and a preparation method thereof. BACKGROUND

[0002] Improper use of chemical pesticides in tea production process can easily lead to excessive pesticide residues, which will make it difficult for enterprises to pass the corresponding detection before the product is put on the market, and will harm the health of consumers and reduce the purchasing desire of consumers, and for foreign export trade, the establishment of various countries' green trade barriers has become the main obstacle for China's tea export, so complete detection procedures are needed before tea is put on the market.

[0003] The traditional detection method mostly uses a full-automatic sample pretreatment system during detection, so that the whole pretreatment time is short and the recovery rate is high, but directly using the full-automatic sample pretreatment system is good for single sample, but if used for simultaneous detection of multiple samples, product cross can easily occur, therefore, the existing partition mixing method is used to distinguish different samples by different extraction, mixing and precipitation in different areas, but such method is only suitable for large type devices with more partitions, and it is difficult to realize the separation between different areas in small devices, and different weight and concentration samples are difficult to distinguish, which can easily cause confusion between different samples, and the part of the fluid diffused can easily spread to the surrounding control group, which is difficult to distinguish, and further can easily affect the data precision of detection.

[0004] Therefore, the present application aims to provide a tea pesticide residue detection sample preparation device and a preparation method thereof, which can realize extraction between different areas in small devices, can simultaneously prepare the same batch and large amount of samples, and can also simultaneously prepare different batches of samples, improve the full-automatic processing efficiency of samples, and further improve the efficiency of the backend detection. SUMMARY

[0005] The present application provides a tea pesticide residue detection sample preparation device and a preparation method thereof, which can effectively solve the above problems.

[0006] The present application is implemented as follows:

[0007] A kind of tea agricultural residues detection with sample preparation device for testing, including: machine table, three-axis gantry mobile frame is provided on the machine table, the three-axis gantry mobile frame is provided with the feeding structure for adding reactant to sample tube, the upper end of the machine table is provided with the oscillation table for placing sample tube, the other side of the feeding structure is also provided with pipe body transfer structure, one side of the machine table is provided with sample transfer frame, the pipe body transfer structure is transferred to sample transfer frame after mixing sample tube, also include:

[0008] Multifunctional linkage structure, the oscillation table includes main oscillation area and several vice oscillation areas separately arranged in the periphery of main oscillation area, the main drive is arranged below the main oscillation area, the lower end of the vice oscillation area is movably mounted with vice drive, the vice drive is connected with vice oscillation area by a connecting piece, the side of vice oscillation area close to main oscillation area is connected with main oscillation area by linkage, when the same sample tube is placed in main oscillation area, the linkage between vice oscillation area and main oscillation area is separated, the main drive drives main oscillation area to rotate alone, when different sample tubes are placed in main oscillation area and vice oscillation area, the linkage between vice oscillation area and main oscillation area is formed by linkage, and the vice drive is separated from vice oscillation area, so that the main oscillation area drives vice oscillation area to rotate when rotating;

[0009] Lower separation structure, including separating piece arranged at the lower end of main oscillation area, the lower end of the separating piece is provided with a lower drive, when the main oscillation area and vice oscillation area are separated, the lower drive pushes the separating piece up and separates between main oscillation area and vice oscillation area.

[0010] As a further improvement, the vice drive includes a vice push rod motor arranged on the machine table, a mating seat is arranged on the output end of the vice push rod motor, the mating seat is a hexagonal structure, and a plurality of clamping points are arranged on the outer side of the mating seat.

[0011] As a further improvement, the inner side of the connecting piece is an internal hexagonal structure, the mating seat is matched with the connecting piece after being pushed out by the vice push rod motor, a plurality of convex points are arranged in the inner part of the connecting piece, and the clamping points and the convex points are at the same height and staggered with each other.

[0012] As a further improvement, the linkage includes a plurality of docking barrels arranged below the main oscillation area, and a docking piece is arranged at the lower end of the vice oscillation area, when the vice oscillation area and the main oscillation area move synchronously, the docking piece is pushed out and matched with the docking barrel.

[0013] As a further improvement, the docking piece includes a docking oil cylinder arranged on the outer side of the connecting piece, a protection cylinder is sleeved on the output end of the docking oil cylinder, and the protection cylinder extends to the inner part of the docking barrel.

[0014] As a further improvement, the protection cylinder is provided with a ring of electromagnets around the output end, which are connected to the electrical appliance outside the docking oil cylinder through a spring wire.

[0015] As a further improvement, the separating member comprises a synchronizing disc connected to the lower driving members, and the synchronizing disc is provided with a mounting hole in the middle for the main driving member, and the synchronizing disc is provided with a plurality of separating frames corresponding to the secondary oscillation area.

[0016] As a further improvement, the separating frame comprises a first separating plate and a second separating plate mounted on the synchronizing disc, the first separating plate is towards the secondary oscillation area, and the second separating plate is towards the main oscillation area.

[0017] The application also provides a tea agricultural residue detection sample preparation method, which uses the tea agricultural residue detection sample preparation device, and comprises the following steps:

[0018] S1: after the tea is crushed at room temperature, the sample is fully shaken to form a test sample, which is placed in a polyethylene bottle, and the test sample is stored at-18 DEG C for standby;

[0019] S2: 2g of the test sample is weighed in a 50mL sample tube, the centrifugal tube is placed in the tea agricultural residue detection sample preparation device, 8mL of ultrapure water is added, and the mixture is vortexed for 0.6min, and then 15mL of acetonitrile and 2 glass homogenizers are added, and the mixture is vortexed for 0.6min, and then 6g of anhydrous magnesium sulfate and 1.5g of sodium acetate are added, and the mixture is shaken for 1min, and then centrifuged at a frequency of 5000r / min for 4min;

[0020] S3: 10mL of the supernatant of S2 is taken to a QuEChERS purification package, vortexed for 1min, and then centrifuged at a frequency of 5000r / min for 3min, and 5mL of the supernatant is accurately taken to a 10mL test tube;

[0021] S4: after the instrument stops, the 10mL test tube of S3 is taken out of the tea agricultural residue detection sample preparation device and placed in a full-automatic parallel concentration instrument, and then nitrogen blowing is performed until it is nearly dry, and then a mixed aqueous solution of methanol and acetonitrile is used to dilute to 1mL;

[0022] S5: 1mL of the liquid is filtered through a 0.22mu m microporous filter membrane and then loaded into a sample vial.

[0023] As a further improvement, the QuEChERS purification package comprises 600mg of anhydrous magnesium sulfate, 50mg of PSA, and 100mg of GCB.

[0024] The application has the following beneficial effects:

[0025] The prior art is difficult to realize the separation between various areas in small equipment, and different weight and different concentration of samples are difficult to distinguish, which leads to easy confusion between different samples, and part of the fluid is easy to diffuse, therefore, through the multifunctional linkage structure arranged, firstly, for the experimental group with the same weight and the same buffer, the main oscillation area and the auxiliary oscillation area are connected through the linkage, and a plurality of test samples of the same type are formed through the single driving mode of the main driving part, secondly, the main oscillation area and the auxiliary oscillation area are separated, and different weight and different buffer control groups are respectively experimented, and centrifugation, vortex and other operations are synchronously performed in the same time period, so that the overall experiment progress is improved, and the effect of large equipment can be realized in small equipment.

[0026] Since the auxiliary oscillation area has two states, the structure for driving the auxiliary oscillation area cannot be in a fixed state, therefore, a connecting piece is arranged at the lower end of the auxiliary oscillation area, and a matching seat is arranged on the auxiliary push rod motor, so that the auxiliary push rod motor is embedded in the area covered by the auxiliary oscillation area when the auxiliary push rod motor needs to cooperate with the auxiliary oscillation area, and the auxiliary push rod motor is separated when the auxiliary oscillation area and the main oscillation area operate synchronously, so that the dual effect is achieved.

[0027] In order to improve the interaction effect between the matching seat and the connecting piece, and avoid separation of the two in the high-speed centrifugal state, the connecting piece is in a hexagonal structure, and the hexagonal structure of the matching seat can better fit when matched, and in order to avoid the edge being rounded due to long-term friction, the connecting piece and the matching seat are respectively provided with convex points and clamping points on the inner and outer sides, so that multi-point friction interference is realized, and the stability of each structure in the high-speed centrifugal state is ensured.

[0028] If the auxiliary oscillation area needs to form a synchronous effect with the main oscillation area, the auxiliary oscillation area needs to be linked with the main oscillation area, therefore, the outer side of the linkage is further provided with a plurality of butt joint cylinders mounted at the lower end of the main oscillation area, and the butt joint cylinders cooperate with the butt joint pieces on the auxiliary oscillation area, so that the two are linked, and the auxiliary oscillation area is driven when the main oscillation area operates.

[0029] In order to realize the connection and separation effect of the butt joint piece, the power of the butt joint piece is arranged as a butt joint oil cylinder, which is more stable than the push rod structure and is not easy to deform, and in order to make the whole matching process more stable, a protection cylinder is arranged on the output end of the butt joint oil cylinder, so as to reduce the stress generated at the contact position, and the upper limit of the centrifugal rate can be improved.

[0030] No matter centrifugal rotation of the main oscillation area itself or centrifugal rotation of the auxiliary oscillation area, is above thousand revolutions per minute, if only through the matching effect of the connecting tube and the protection tube itself, the auxiliary oscillation area and the main oscillation area are easily separated, therefore, the electromagnet is arranged outside the protection tube, the whole auxiliary oscillation area is tightly adsorbed on the main oscillation area through the strong attraction of the metal connecting tube, and can be kept in a relatively stable state even in the state of above thousand revolutions per minute.

[0031] But in the process of different group control experiments, the sample tube needs to be open because different liquids need to be continuously added in the process, but in the centrifugal state, part of volatile substances may volatilize, which will affect other group experiments, therefore, the lower separation structure is arranged on the basis of the multifunctional linkage structure, the separating piece is ejected by the lower driving piece when the main oscillation area and the auxiliary oscillation area realize their different functions, the separation between the two areas is realized, the different test areas are not affected each other, the precision of the single sample is ensured, and the precision of the subsequent detection is ensured.

[0032] In the separating process of the separating piece, not only a separation section is formed, but also a separation section is formed in the area of all auxiliary oscillation areas, so that it is not necessary to judge which processing position the control group is in, therefore, the separating frame in the separating piece is arranged on the synchronous disc, the synchronous disc can be driven by the driving piece, all separating frames are simultaneously separated between the auxiliary oscillation area and the main oscillation area, and the establishment of the separation surface is completed.

[0033] In the separating process, not only the auxiliary oscillation area may affect the main oscillation area, but also the main oscillation area may affect the main oscillation area, therefore, the two separation plates of the separating frame are arranged in the arc-shaped structure, and are adapted to the auxiliary oscillation area and the main oscillation area, so that the rotating state is not affected, and the separating effect is achieved. BRIEF DESCRIPTION OF DRAWINGS

[0034] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the drawings needed to be used in the embodiments will be briefly introduced as follows, and it should be understood that the following drawings only show some embodiments of the present application, and should not be regarded as a limitation to the scope, and other related drawings can be obtained by those skilled in the art without creative labor on the basis of the drawings.

[0035] Figure 1 It is an external structure schematic view of the present application.

[0036] Figure 2 It is an internal structure schematic view of the present application.

[0037] Figure 3 It is an internal structure schematic view of the present application Figure 2is a front view of the present application.

[0038] Figure 4 is a structural schematic diagram of the oscillation table and the multifunction linkage structure of the present application.

[0039] Figure 5 is a top view of the present application. Figure 4

[0040] Figure 6 is a sectional view of A-A in the present application. Figure 5

[0041] Figure 7 is a bottom structural schematic diagram of the docking piece of the present application.

[0042] Figure 8 is a structural schematic diagram of the lower separation structure of the present application.

[0043] In the figure:

[0044] Machine table 10, three-axis gantry moving frame 20, feeding structure 30, oscillation table 40, main oscillation area 41, auxiliary oscillation area 42, pipe body transfer structure 50, sample transfer frame 60, multifunction linkage structure 70, main driving piece 71, auxiliary driving piece 72, auxiliary push rod motor 721, matching seat 722, clamping point 723, connecting piece 73, docking cylinder 741, docking piece 742, docking oil cylinder 7421, output end 7422, protection cylinder 7423, electromagnet 7424, elastic lead 7425, electric appliance 7426, convex point 733, lower separation structure 80, separation piece 81, synchronous disc 811, mounting hole 812, separation frame 813, first separation plate 8131, second separation plate 8132, lower driving piece 82. DETAILED DESCRIPTION

[0045] In order to implement the embodiments of the present application, all belong to the scope of protection of the present application. Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor also belong to the scope of protection of the present application.

[0046] ​​In the description of the present application, the terms "first", "second" are only for the purpose of description, and cannot be understood as the purpose of indicating the manner, technical solutions and advantages are clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments or implied relative importance or implicitly indicated the number of technical features obtained by those skilled in the art without creative labor. Therefore, the features with "first", "second" can be explicitly or implicitly included one or more features. In the description of the present application, the meaning of "a plurality of" is two or more, unless otherwise specifically limited.

[0047] Referring to Figures 1-8 As shown in FIG. 1, a kind of tea leaf pesticide residue detection sample preparation device for measured sample, including: machine table 10, three-axis gantry moving frame 20 is provided on the machine table 10, the three-axis gantry moving frame 20 is provided with the feeding structure 30 of adding reactant to sample tube, the upper end of the machine table 10 is provided with the oscillation table 40 for placing sample tube, the other side of the feeding structure 30 is also provided with pipe body transfer structure 50, one side of the machine table 10 is provided with sample transfer frame 60, the pipe body transfer structure 50 is transferred to sample transfer frame 60 after mixing sample tube, further comprising: multifunction linkage structure 70, the oscillation table 40 includes main oscillation area 41 and several vice oscillation areas 42 separately arranged in the periphery of main oscillation area 41, the multifunction linkage structure 70 includes main drive 71 arranged below main oscillation area 41, vice drive 72 is movably installed at the lower end of vice oscillation area 42, vice drive 72 is connected with vice oscillation area 42 by a connecting piece 73, the side of vice oscillation area 42 close to main oscillation area 41 is connected with main oscillation area 41 by linkage 74, when the same sample tube is placed in main oscillation area 41, the linkage 74 between vice oscillation area 42 and main oscillation area 41 is separated, the main drive 71 drives main oscillation area 41 to rotate alone, when different sample tubes are placed in main oscillation area 41 and vice oscillation area 42, linkage 74 is formed between vice oscillation area 42 and main oscillation area 41, and vice drive 72 is separated from vice oscillation area 42, so that main oscillation area 41 drives vice oscillation area 42 to rotate when rotating; lower separation structure 80 includes separation piece 81 arranged at the lower end of main oscillation area 41, lower drive 82 is arranged at the lower end of separation piece 81, when main oscillation area 41 and vice oscillation area 42 are separated, lower drive 82 pushes separation piece 81 up and separates between main oscillation area 41 and vice oscillation area 42.

[0048] In the preparation stage, the initial tea leaves are placed on the oscillation table 40, and the substances added during the preparation are all added through the feeding structure 30, and after the preparation process is completed, the sample tube is transferred to the sample transfer rack 60 through the tube transfer structure 50, and waits for the next stage to be taken.

[0049] The prior art is difficult to realize the separation between the regions in a small device, and the samples with different weights and different concentrations are difficult to distinguish, so that confusion between different samples is prone to occur, and part of the fluid is prone to diffuse, therefore, the multifunctional linkage structure 70 is arranged, firstly, for the experimental groups with the same weight and the same buffer, the main oscillation area 41 and the auxiliary oscillation area 42 are connected through the linkage 74, and are driven in a single driving mode through the main driving part 71, so that a plurality of test samples of the same type are formed at the same time, and secondly, the main oscillation area 41 and the auxiliary oscillation area 42 are separated, and the control groups with different weights and different buffers are respectively experimented, and the centrifugation, vortex and other operations are synchronously performed in the same time period, so that the overall experimental progress is improved, and the effect of a large device can be realized in a small device.

[0050] Since the auxiliary oscillation area 42 has two states, the structure for driving the auxiliary oscillation area 42 cannot be in a fixed state, therefore, the auxiliary driving part 72 comprises an auxiliary push rod motor 721 arranged on the machine table 10, a matching seat 722 is arranged on the output end of the auxiliary push rod motor 721, the matching seat 722 is a hexagonal structure, a plurality of clamping points 723 are arranged on the outer side of the matching seat 722, a connecting piece 73 is arranged at the lower end of the auxiliary oscillation area 42, and the matching seat 722 is arranged on the auxiliary push rod motor 721, so that the auxiliary push rod motor 721 is embedded in the area covered by the auxiliary oscillation area 42 when the auxiliary push rod motor 721 needs to cooperate with the auxiliary oscillation area 42, and the auxiliary push rod motor 721 is separated when the auxiliary oscillation area 42 and the main oscillation area 41 operate synchronously, so that the dual-purpose effect is achieved.

[0051] In order to improve the interaction effect between the matching seat 722 and the connecting piece 73, and avoid separation of the matching seat 722 and the connecting piece 73 in the high-speed centrifugal state, the inner side of the connecting piece 73 is an internal hexagonal structure, the matching seat 722 is matched with the connecting piece 73 after being pushed out by the auxiliary push rod motor 721, a plurality of convex points 733 are arranged in the connecting piece 73, the clamping points 723 and the convex points 733 are located at the same height and are staggered with each other, the connecting piece 73 is an internal hexagonal structure, and the hexagonal structure of the matching seat 722 can better fit when matched, and in order to avoid that the edges are rounded due to long-term friction, the convex points 733 and the clamping points 723 are arranged on the inner and outer sides of the connecting piece 73 and the matching seat 722 respectively, multi-point friction interference is realized, and the stability of each structure in the high-speed centrifugal state is ensured.

[0052] The sub-oscillation area 42 needs to be linked with the main oscillation area 41 if synchronization effect is needed, therefore, the linkage 74 of the embodiment comprises a plurality of docking tubes 741 arranged below the main oscillation area 41, the lower end of the sub-oscillation area 42 is provided with a docking piece 742, when the sub-oscillation area 42 moves synchronously with the main oscillation area 41, the docking piece 742 is pushed out and cooperates with the docking tube 741, the outer side of the linkage 74 is further provided with a plurality of docking tubes 741 installed at the lower end of the main oscillation area 41, cooperating with the docking piece 742 on the sub-oscillation area 42, so that the two can form a certain linkage, and then the sub-oscillation area 42 can be driven when the main oscillation area 41 moves.

[0053] In order to realize the engagement and separation effect of the docking piece 742, the docking piece 742 of the embodiment comprises a docking oil cylinder 7421 arranged on the outer side of the connecting piece 73, a protection tube 7423 is sleeved on the output end 7422 of the docking oil cylinder 7421, the protection tube 7423 extends to the inside of the docking tube 741, the power of the docking piece 742 is arranged as the docking oil cylinder 7421, which is more stable than the push rod structure and is not easy to deform, in order to make the whole cooperation process more stable, the protection tube 7423 is arranged on the output end 7422 of the docking oil cylinder 7421, so as to reduce the stress generated at the contact position, and then the upper limit of the centrifugal rate can be improved.

[0054] Whether it is the centrifugal rotation of the main oscillation area 41 itself or the centrifugal rotation of the sub-oscillation area 42, it is above thousands of revolutions per minute, if only the cooperation effect of the docking tube 741 and the protection tube 7423 itself, it is easy to separate the sub-oscillation area 42 from the main oscillation area 41, therefore, the protection tube 7423 of the embodiment is provided with an electromagnet 7424 sleeved on the output end 7422 on the side close to the docking tube 741, the electromagnet 7424 is connected to the electric appliance 7426 outside the docking oil cylinder 7421 through a elastic lead 7425, by arranging the electromagnet 7424 on the outer side of the protection tube 7423, by strongly attracting the metal docking tube 741, the whole sub-oscillation area 42 is tightly adsorbed on the main oscillation area 41, even in the state of thousands of revolutions per minute, it can be kept in a relatively stable state.

[0055] But in the process of different group control experiment, because different liquid needs to be added constantly in the process, so the sample tube needs to be open, but in the centrifugal state, it may cause part of volatile material to volatilize, which may affect other group experiments, therefore, the lower separation structure 80 is arranged on the basis of the multifunctional linkage structure 70, when the main oscillation area 41 and the auxiliary oscillation area 42 realize different functions, the separation piece 81 is pushed out by the lower driving piece 82, the separation between the two areas is realized, and then the different test areas do not affect each other, and then the precision of the single sample is ensured, so that the precision of the subsequent detection is ensured.

[0056] In the separation process of the separation piece 81, not only a separation section is formed, but also a separation section is formed in the area of all auxiliary oscillation areas 42, so that it is not necessary to judge which processing position the control group is in, therefore, the separation piece 81 of the embodiment comprises a synchronous disc 811 connected with a plurality of lower driving pieces 82, a mounting hole 812 for the main driving piece 71 is arranged in the middle of the synchronous disc 811, a plurality of separation frames 813 are arranged on the synchronous disc 811 at positions corresponding to the auxiliary oscillation areas 42, the separation frames 813 in the separation piece 81 are arranged on the synchronous disc 811, the synchronous disc 811 can be synchronously driven by the driving piece 82, so that all the separation frames 813 are simultaneously separated between the auxiliary oscillation areas 42 and the main oscillation area 41, and then the separation surface is established.

[0057] In the separation process, not only the auxiliary oscillation area 42 may affect the main oscillation area 41, but also the main oscillation area 41 may affect the main oscillation area 41, therefore, the separation frame 813 of the embodiment comprises a first separation plate 8131 and a second separation plate 8132 arranged on the synchronous disc 811, the first separation plate 8131 faces the auxiliary oscillation area 42, the second separation plate 8132 faces the main oscillation area 41, the two separation plates of the separation frame 813 are arranged in an arc-shaped structure, and are adapted to the auxiliary oscillation area 42 and the main oscillation area 41, so as not to affect the rotation state, and at the same time, the separation effect is achieved.

[0058] Another embodiment of the application also discloses a tea agricultural residue detection sample preparation method, which applies the above-mentioned tea agricultural residue detection sample preparation device, and is characterized by comprising the following steps:

[0059] S1: after the tea is crushed at room temperature, the sample is shaken uniformly, put into a polyethylene bottle, and stored at-18 DEG C for standby;

[0060] S2: weigh 2g sample in 50mL sample tube, put the centrifuge tube into the tea pesticide residue detection sample preparation device, add 8 mL ultrapure water, vortex for 0.6 min, stand for 30 min, add 15 mL acetonitrile and 2 glass homogenizer, vortex for 0.6 min, then add 6 g anhydrous magnesium sulfate and 1.5 g sodium acetate, shake for 1 min, then centrifuge at a frequency of 5000r / min for 4 min;

[0061] S3: take 10 mL supernatant of S2 into the QuEChERS purification package, vortex for 1 min, then centrifuge at a frequency of 5000r / min for 3 min, and accurately take 5 mL supernatant into a 10 mL test tube;

[0062] S4: after the instrument stops, take S3 10 mL test tube out of the tea pesticide residue detection sample preparation device, put it into the full-automatic parallel concentration instrument, nitrogen blow to nearly dry, then use the mixed aqueous solution of methanol and acetonitrile to constant volume to 1 mL;

[0063] S5: take 1 mL liquid through 0.22μm microporous filter membrane, then put it into the sample vial.

[0064] Further, the QuEChERS purification package contains 600 mg anhydrous magnesium sulfate, 50 mg PSA, 100 mg GCB.

[0065] The above only describes the preferred embodiments of the present application and is not used to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A tea leaf agricultural residue detection test sample preparation device, characterized by, The utility model relates to a kind of multi-functional sample preparation device, including: Machine table (10), the machine table (10) is provided with three-axis gantry moving frame (20), the three-axis gantry moving frame (20) is provided with the feeding structure (30) of adding reactant to sample tube, the upper end of the machine table (10) is provided with the oscillation table (40) for placing sample tube, the other side of the feeding structure (30) is further provided with tube body transfer structure (50), one side of the machine table (10) is provided with sample transfer frame (60), the tube body transfer structure (50) is transferred to sample transfer frame (60) after mixing sample tube, further including: Multifunctional linkage structure (70), the oscillation table (40) includes main oscillation area (41) and several sub-oscillation areas (42) separately arranged in the periphery of main oscillation area (41), the multifunctional linkage structure (70) includes main drive (71) arranged below main oscillation area (41), the lower end of the sub-oscillation area (42) is movably mounted with sub-drive (72), the sub-drive (72) is connected with sub-oscillation area (42) by a connecting piece (73), the side of the sub-oscillation area (42) close to main oscillation area (41) is connected with main oscillation area (41) by linkage (74), when the same sample tube is placed in main oscillation area (41), the linkage (74) between the sub-oscillation area (42) and main oscillation area (41) is separated, the main drive (71) drives main oscillation area (41) to rotate alone, when different sample tubes are placed in main oscillation area (41) and sub-oscillation area (42), the sub-oscillation area (42) and main oscillation area (41) are linked by linkage (74), and the sub-drive (72) is separated from the sub-oscillation area (42), so that the main oscillation area (41) drives the sub-oscillation area (42) to rotate when rotating; Lower separation structure (80) includes separation piece (81) arranged at the lower end of main oscillation area (41), a lower drive (82) is arranged at the lower end of the separation piece (81), when the main oscillation area (41) and the sub-oscillation area (42) are separated, the lower drive (82) pushes up the separation piece (81) and separates between main oscillation area (41) and sub-oscillation area (42).

2. The device for preparing a sample to be tested for detecting pesticide residues in tea leaves according to claim 1, characterized in that, The sub-drive (72) includes sub-push rod motor (721) arranged on the machine table (10), a matching seat (722) is arranged on the output end of the sub-push rod motor (721), the matching seat (722) is hexagonal structure, a plurality of clamping points (723) are arranged on the outer side of the matching seat (722). 3.The tea agricultural residue detection sample preparation device according to claim 2, characterized in that, The inner side of the connecting piece (73) is an internal hexagonal structure, the matching seat (722) is matched with the connecting piece (73) after being pushed out by the sub-push rod motor (721), a plurality of convex points (733) are arranged in the connecting piece (73), the clamping points (723) and the convex points (733) are at the same height and staggered with each other.

4. The device for preparing a sample to be tested for detecting pesticide residues in tea leaves according to claim 3, characterized in that, The linkage (74) comprises a plurality of docking sleeves (741) arranged below the main oscillation area (41), and the lower end of the auxiliary oscillation area (42) is provided with a docking piece (742), which is pushed out and matched with the docking sleeve (741) when the auxiliary oscillation area (42) moves synchronously with the main oscillation area (41).

5. The device for preparing a sample to be tested for detecting pesticide residues in tea leaves according to claim 4, characterized in that, The docking piece (742) comprises a docking oil cylinder (7421) arranged outside the connecting piece (73), and a protection sleeve (7423) is sleeved on the output end (7422) of the docking oil cylinder (7421), which extends to the inside of the docking sleeve (741).

6. The device for preparing a sample to be tested for detecting pesticide residues in tea leaves according to claim 5, characterized in that, The protection sleeve (7423) is provided with an electromagnet (7424) sleeved on the output end (7422) near one side of the docking sleeve (741), and the electromagnet (7424) is connected to an electric appliance (7426) outside the docking oil cylinder (7421) through a resilient lead (7425).

7. The device for preparing a sample to be tested for detecting pesticide residues in tea leaves according to claim 6, characterized in that, The separation piece (81) comprises a synchronization disc (811) connected with a plurality of lower driving pieces (82), and the middle part of the synchronization disc (811) is provided with a mounting hole (812) for the main driving piece (71), and a plurality of separation frames (813) are arranged on the synchronization disc (811) corresponding to the position of the auxiliary oscillation area (42). 8.The tea agricultural residue detection sample preparation device according to claim 7, characterized in that, The separation frame (813) comprises a first separation plate (8131) and a second separation plate (8132) mounted on the synchronization disc (811), the first separation plate (8131) faces the auxiliary oscillation area (42), and the second separation plate (8132) faces the main oscillation area (41). 9.A method for preparing a sample to be tested for detecting pesticide residues in tea leaves, using the device for preparing a sample to be tested for detecting pesticide residues in tea leaves according to any one of claims 1-8, characterized in that, The method comprises the following steps: S1: After the tea leaves are crushed at room temperature, the sample is shaken well, put into a polyethylene bottle, and stored at-18℃ for standby; S2: 2g of the sample is weighed in a 50mL sample tube, the centrifugal tube is placed in the tea residue detection sample preparation device, 8mL of ultrapure water is added, and the mixture is vortexed for 0.6min, then the mixture is left for 30min, 15mL of acetonitrile and 2 glass homogenizers are added, the mixture is vortexed for 0.6min, then 6g of anhydrous magnesium sulfate and 1.5g of sodium acetate are added, the mixture is shaken for 1min, and then the mixture is centrifuged at a frequency of 5000r / min for 4min; S3: 10mL of the supernatant of S2 is taken to the QuEChERS purification package, the mixture is vortexed for 1min, then the mixture is centrifuged at a frequency of 5000r / min for 3min, and 5mL of the supernatant is accurately taken to a 10mL test tube; S4: After the instrument stops, the 10mL test tube of S3 is taken out of the tea residue detection sample preparation device and placed in the full-automatic parallel concentration instrument, and then the mixture is nitrogen-blowing to near dryness, and then the mixture is diluted to 1mL with a mixed aqueous solution of methanol and acetonitrile; S5: 1mL of the liquid is filtered through a 0.22μm microporous filter membrane and then loaded into an injection vial. 10.The method for preparing a sample for detecting pesticide residues in tea leaves according to claim 9, characterized in that, The QuEChERS purification package comprises 600mg of anhydrous magnesium sulfate, 50mg of PSA, and 100mg of GCB.

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