Biochemical composite rice seed soaking agent and application thereof in rice brown rice cadmium resistance

Abstract

The application discloses a biochemical compound rice seed soaking agent and application thereof in resisting cadmium in rice brown rice, and belongs to the field of agricultural technology; components of the biochemical compound rice seed soaking agent are as follows: the concentration of hematin chloride is 10-50 micromol / L; the concentration of calcium chloride is 1-3 mmol / L; the concentration of alpha-ketoglutaric acid is 0.5-2 mmol / L; the concentration of sodium dodecyl sulfate (SDS) is 5-8 mmol / L; and water is used as a solvent; after rice seeds are soaked in the compound seed soaking agent for 12-24 hours, the rice seeds are taken out and naturally dried, and then germination is waited for; after the germination reaches suitable sowing conditions, the rice seeds are sowed in a seedling field; after seedling is raised to reach suitable planting conditions, seedlings are transplanted to a cadmium-polluted field, and the cadmium accumulation amount of cadmium-polluted rice brown rice can be significantly reduced; in the application, the synergistic effect of hematin chloride, calcium chloride and alpha-ketoglutaric acid can greatly reduce the absorption capacity of rice to heavy metal cadmium in soil when rice seeds are soaked, and the cadmium reduction efficiency can reach more than 80%.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of agricultural technology, in particular to a biochemical compound rice seed soaking agent and its application in rice brown rice cadmium resistance. BACKGROUND

[0002] Current response to cadmium pollution of rice mainly relies on soil remediation technology, including guest soil method, chemical washing method and soil amendment addition, etc. These methods have certain limitations: guest soil method, through physical replacement of contaminated soil layer to achieve treatment, but has problems of large engineering quantity, high cost and damage to soil ecology; chemical washing method, using EDTA and other chelating agents (50-100 kg / acre) to extract heavy metals, which is easy to cause groundwater pollution (COD exceeds standard by 12-15 times) and the removal rate of combined cadmium is less than 40%; soil amendment and passivation agent addition, using materials such as sepiolite to fix heavy metals, but the effect is significantly reduced in acidic soil (pH<5.5), and the effective period is short. Therefore, the existing means cannot meet the practical needs.

[0003] In contrast, seed treatment technology as a source control means has more potential, seed soaking agent plays a role through physiological blocking mechanism, but the existing seed soaking technology has problems such as limited effect of single agent, complex composition of compound seed soaking agent, poor synergy of compound formula, and even affects the normal germination of seeds.

[0004] Therefore, it is urgent to develop a high-efficiency and simple-to-use compound seed soaking agent to effectively reduce the cadmium absorption capacity of rice in the early growth stage, so as to more simply and efficiently reduce the cadmium content of rice brown rice. SUMMARY

[0005] The present application aims to overcome the shortcomings of the prior art and provides a biochemical compound rice seed soaking agent and its application in rice brown rice cadmium resistance, which belongs to the field of agricultural technology. The components of the biochemical compound rice seed soaking agent are as follows: the concentration of chlorinated hematin is 10-50 μmol / L; the concentration of calcium chloride is 1-3 mmol / L; the concentration of alpha-ketoglutaric acid is 0.5-2 mmol / L; the concentration of sodium dodecyl sulfate SDS is 5-8 mmol / L; the solvent is water; the rice seeds are soaked in the compound seed soaking agent for 12-24 hours, then the rice seeds are taken out, naturally dried, and germinated, and the rice seeds are sown in the seedling field after the germination reaches the suitable sowing conditions, and the seedlings are transplanted to the cadmium contaminated field, which can significantly reduce the cumulative amount of cadmium in cadmium contaminated rice brown rice; in the present application, the synergistic effect of chlorinated hematin, calcium chloride and alpha-ketoglutaric acid can greatly reduce the absorption capacity of rice to heavy metal cadmium in the soil during rice seed soaking, and the cadmium reduction efficiency can reach more than 80%.

[0006] To achieve the above technical effects, the following technical solutions are adopted:

[0007] A biochemical complex rice seed soaking agent, comprising the following components:

[0008] The agent and the solvent;

[0009] The agent is: hematin chloride; calcium chloride; alpha-ketoglutaric acid and sodium dodecyl sulfate SDS; and the solvent is water;

[0010] The component formula of the biochemical complex rice seed soaking agent is:

[0011] The concentration of hematin chloride is 10-50 μmol / L; the concentration of calcium chloride is 1-3 mmol / L; the concentration of alpha-ketoglutaric acid is 0.5-2 mmol / L; and the concentration of sodium dodecyl sulfate SDS is 5-8 mmol / L;

[0012] Further, the agent is a powder or other preparation.

[0013] Further, the component formula of the biochemical complex rice seed soaking agent is:

[0014] The concentration of hematin chloride is 30 μmol / L; the concentration of calcium chloride is 2 mmol / L; the concentration of alpha-ketoglutaric acid is 1 mmol / L; and the concentration of sodium dodecyl sulfate SDS is 6 mmol / L; and the balance is water.

[0015] A preparation method of a biochemical complex rice seed soaking agent, specifically comprising:

[0016] Hematin chloride, calcium chloride, alpha-ketoglutaric acid, sodium dodecyl sulfate SDS and water are weighed according to the concentration requirements and preparation amount; sodium dodecyl sulfate SDS is first dissolved in water, then hematin chloride is added, and after uniform stirring, the mixture is left to stand for 10-30 minutes, and then calcium chloride and alpha-ketoglutaric acid are added in sequence, and the mixture is stirred and dissolved to obtain the biochemical complex rice seed soaking agent.

[0017] A seed soaking method of a biochemical complex rice seed soaking agent, specifically comprising: soaking rice seeds in the biochemical complex rice seed soaking agent; and the use amount of the biochemical complex rice seed soaking agent is that the seed soaking agent should completely cover the rice seeds during seed soaking, and the rice seeds are completely soaked.

[0018] Further, the seed soaking time is 12-24 hours, and the rice varieties include but are not limited to Ge Liangyou series, Xinganxiachang and Qiliangyou.

[0019] Further, after the seed soaking is completed, the seeds are naturally dried, germination is waited, and after the germination reaches the suitable sowing conditions, the rice seeds are sown in a nursery, and the seedlings are transplanted to a cadmium-polluted field after the seedling raising reaches the suitable transplanting conditions.

[0020] Further, the seed soaking temperature is 25-30°C.

[0021] Further, the application of the above-mentioned biochemical composite rice seed soaking agent to the cadmium resistance of rice brown rice.

[0022] The beneficial effects of the present application are:

[0023] The application discloses a biochemical composite rice seed soaking agent and application thereof to the cadmium resistance of rice brown rice, and belongs to the field of agricultural technologies. The components of the biochemical composite rice seed soaking agent are as follows: the concentration of hematin chloride is 10-50 micromoles per liter; the concentration of calcium chloride is 1-3 millimoles per liter; the concentration of alpha-ketoglutaric acid is 0.5-2 millimoles per liter; the concentration of sodium dodecyl sulfate (SDS) is 5-8 millimoles per liter; and the solvent is water. After rice seeds are soaked in the composite seed soaking agent for 12-24 hours, the rice seeds are taken out and naturally dried, and then are sowed in a seedling field after germination reaches the suitable sowing condition. After seedling is cultivated to reach the suitable planting condition, the seedlings are transplanted into a cadmium-polluted field, so that the cumulative amount of cadmium in the cadmium-polluted rice brown rice can be significantly reduced. In the application, the synergistic effect of hematin chloride, calcium chloride and alpha-ketoglutaric acid is utilized. The composite system realizes the source control of cadmium absorption through the triple synergism of hematin chloride antioxidant defense, calcium chloride ion antagonism and transport pathway competition, and alpha-ketoglutaric acid metabolic regulation and activation. Meanwhile, the solubility of hematin chloride in water is increased through sodium dodecyl sulfate (SDS), so that hematin chloride is smoothly dispersed and dissolved in water. In addition, the bactericidal effect of sodium dodecyl sulfate (SDS) ensures the seed germination rate. When rice seeds are soaked, the absorption capacity of rice for heavy metal cadmium in the soil can be greatly reduced, and the cadmium reduction efficiency can reach more than 80%. DETAILED DESCRIPTION

[0024] In order to make the objects, technical solutions and advantages of the present application clearer, the present application will be further described in detail below with reference to examples. It should be understood that the specific examples described herein are only used to explain the present application and not intended to limit the present application.

[0025] It should be pointed out that the following detailed description is exemplary and is intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as generally understood by those skilled in the art to which the present application belongs.

[0026] It should be noted that the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, the singular form is intended to include the plural form unless the context clearly indicates otherwise, and furthermore, it should be understood that when the terms "comprise" and / or "include" are used in the specification, there is a presence of the features, steps, operations and / or combinations thereof.

[0027] Example 1

[0028] The concentration of hematin chloride is 10 μmol / L; the concentration of calcium chloride is 1 mmol / L; the concentration of α-ketoglutaric acid is 0.5 mmol / L; the concentration of sodium dodecyl sulfate SDS is 5 mmol / L; and the balance is water.

[0029] For 100 L of the compound seed soaking agent, hematin chloride is 0.652 g; calcium chloride is 11.1 g; α-ketoglutaric acid is 7.3 g; sodium dodecyl sulfate SDS is 144.2 g; and the balance is water.

[0030] According to the above concentration requirements and preparation amount, hematin chloride, calcium chloride, α-ketoglutaric acid, sodium dodecyl sulfate SDS and water are weighed; sodium dodecyl sulfate SDS is dissolved in water, then hematin chloride is added, and after stirring evenly, hematin chloride is allowed to stand for 10-30 minutes, and then calcium chloride and α-ketoglutaric acid are added in sequence, and the mixture is stirred and dissolved evenly to obtain the biochemical compound rice seed soaking agent 1.

[0031] Example 2

[0032] The concentration of hematin chloride is 50 μmol / L; the concentration of calcium chloride is 3 mmol / L; the concentration of α-ketoglutaric acid is 2 mmol / L; the concentration of sodium dodecyl sulfate SDS is 8 mmol / L; and the balance is water.

[0033] For 100 L of the compound seed soaking agent, hematin chloride is 3.26 g; calcium chloride is 33.3 g; α-ketoglutaric acid is 29.2 g; sodium dodecyl sulfate SDS is 230.7 g; and the balance is water.

[0034] According to the above concentration requirements and preparation amount, hematin chloride, calcium chloride, α-ketoglutaric acid, sodium dodecyl sulfate SDS and water are weighed; sodium dodecyl sulfate SDS is dissolved in water, then hematin chloride is added, and after stirring evenly, hematin chloride is allowed to stand for 10-30 minutes, and then calcium chloride and α-ketoglutaric acid are added in sequence, and the mixture is stirred and dissolved evenly to obtain the biochemical compound rice seed soaking agent 2.

[0035] Example 3

[0036] The concentration of hematin chloride is 30 μmol / L; the concentration of calcium chloride is 2 mmol / L; the concentration of α-ketoglutaric acid is 1 mmol / L; the concentration of sodium dodecyl sulfate SDS is 6 mmol / L; and the balance is water.

[0037] For 100 L of the compound seed soaking agent, hematin chloride is 1.956 g; calcium chloride is 22.2 g; α-ketoglutaric acid is 14.6 g; sodium dodecyl sulfate SDS is 173.0 g; and the balance is water.

[0038] According to the concentration requirements and preparation amount above, hematin chloride, calcium chloride, alpha-ketoglutaric acid, sodium dodecyl sulfate SDS and water are weighed; sodium dodecyl sulfate SDS is dissolved in water, then hematin chloride is added, stirred and uniformly dissolved, and then uniformly dissolved after standing for 10-30 minutes, and then calcium chloride and alpha-ketoglutaric acid are sequentially added and stirred and uniformly dissolved to obtain the biochemical compound rice seed soaking agent 3.

[0039] All the comparative examples are based on Example 3.

[0040] Comparative Example 1:

[0041] For example, 100L of the compound seed soaking agent, hematin chloride: 38.756g; sodium dodecyl sulfate SDS: 173.0g; the rest is water.

[0042] According to the concentration requirements and preparation amount above, hematin chloride, sodium dodecyl sulfate SDS and water are weighed; sodium dodecyl sulfate SDS is dissolved in water, then hematin chloride is added, stirred and uniformly dissolved, and then uniformly dissolved after standing for 10-30 minutes, and then stirred and uniformly dissolved or dispersed to obtain the rice seed soaking agent 4.

[0043] Comparative Example 2:

[0044] For example, 100L of the compound seed soaking agent, calcium chloride: 38.756g; sodium dodecyl sulfate SDS: 173.0g; the rest is water.

[0045] According to the concentration requirements and preparation amount above, calcium chloride, sodium dodecyl sulfate SDS and water are weighed; sodium dodecyl sulfate SDS is dissolved in water, then calcium chloride is added, stirred and uniformly dissolved to obtain the rice seed soaking agent 5.

[0046] Comparative Example 3:

[0047] For example, 100L of the compound seed soaking agent, alpha-ketoglutaric acid: 38.756g; sodium dodecyl sulfate SDS: 173.0g; the rest is water.

[0048] According to the concentration requirements and preparation amount above, alpha-ketoglutaric acid, sodium dodecyl sulfate SDS and water are weighed; sodium dodecyl sulfate SDS is dissolved in water, then alpha-ketoglutaric acid is added, stirred and uniformly dissolved to obtain the rice seed soaking agent 6.

[0049] Comparative Example 4:

[0050] For example, 100L of the compound seed soaking agent, hematin chloride: 1.956g; calcium chloride: 36.8g; sodium dodecyl sulfate SDS: 173.0g; the rest is water.

[0051] According to the concentration requirements and preparation amount above, hematin chloride, calcium chloride, sodium dodecyl sulfate SDS and water are weighed; sodium dodecyl sulfate SDS is dissolved in water, then hematin chloride is added, after fully stirring and uniform, standing for 10-30 minutes, calcium chloride is added in sequence, stirring and dissolving uniformly to obtain rice seed soaking agent 7.

[0052] Comparative example 5:

[0053] For example, 100L of the composite seed soaking agent, hematin chloride: 1.956g; alpha-ketoglutaric acid: 36.8g; sodium dodecyl sulfate SDS: 173.0g; the rest is water.

[0054] According to the concentration requirements and preparation amount above, hematin chloride, alpha-ketoglutaric acid, sodium dodecyl sulfate SDS and water are weighed; sodium dodecyl sulfate SDS is dissolved in water, then hematin chloride is added, after fully stirring and uniform, standing for 10-30 minutes, alpha-ketoglutaric acid is added in sequence, stirring and dissolving uniformly to obtain rice seed soaking agent 8.

[0055] Comparative example 6:

[0056] For example, 100L of the composite seed soaking agent, calcium chloride: 23.38g; alpha-ketoglutaric acid: 15.38g; sodium dodecyl sulfate SDS: 173.0g; the rest is water.

[0057] According to the concentration requirements and preparation amount above, calcium chloride, alpha-ketoglutaric acid, sodium dodecyl sulfate SDS and water are weighed; sodium dodecyl sulfate SDS is dissolved in water, then calcium chloride and alpha-ketoglutaric acid are added in sequence, stirring and dissolving uniformly to obtain rice seed soaking agent 9.

[0058] Comparative example 7:

[0059] For example, 100L of the composite seed soaking agent, sodium dodecyl sulfate SDS: 173.0g; the rest is water.

[0060] According to the concentration requirements and preparation amount above, sodium dodecyl sulfate SDS and water are weighed; sodium dodecyl sulfate SDS is dissolved in water, stirring and dissolving uniformly to obtain rice seed soaking agent 10.

[0061] Comparative example 8:

[0062] For example, 100L of the composite seed soaking agent, all is water, recorded as rice seed soaking agent 11.

[0063] The seed soaking agents in examples 1-3 and comparative examples 1-8 are evaluated as follows:

[0064] Cd reduction effect verification test: field plot comparison test is carried out on the seed soaking agents in examples 1-3 and comparative examples 1-8. The test process is as follows:

[0065] Test site: Binjiang Town, Guixi City, Yingtan City

[0066] Test field: The total cadmium content of the soil was 0.53-0.58 mg / kg, the pH was 5.47-5.52, the available cadmium content was 0.29-0.31 mg / kg, and the rice variety was uniform, which was a local main variety (Xinganxiang).

[0067] Test time: Late rice season in 2024

[0068] Specifically:

[0069] According to the size of the test field, an appropriate number of rice seeds were selected, and the same number of rice seeds were soaked in the seed soaking agents of Examples 1-3 and Comparative Examples 1-8. The seed soaking agent should completely cover the rice seeds during seed soaking, and the rice seeds should be completely soaked. After 24 hours of seed soaking at a temperature of 27±1°C, the soaked seeds were taken out and dried, and natural germination was performed at an environmental temperature of 22±1°C. The seeds were turned over as appropriate to prevent rotting.

[0070] After the rice seedlings reached a length of more than 1.5 cm, the rice seeds were planted in the nursery bed, and the nursery bed was normally cultivated. After the nursery bed reached the suitable conditions for planting, the seedlings were transplanted to the cadmium-contaminated field.

[0071] Nursery test setup: Examples 1-3 and Comparative Examples 1-8 above were single nursery areas, and the entire nursery field was divided into 44 plots. All plots were in the same field, had the same growth environment, and had the same soil fertility. Every 4 plots were a nursery area (4 repeated experiments), and there were 11 nursery areas. Nursery areas 1-11 were used for the nursery of rice seeds soaked in the seed soaking agents of Examples 1-3 and Comparative Examples 1-8, respectively.

[0072] Transplanting test setup: Examples 1-3 and Comparative Examples 1-8 above were single growth area transplanting growth, and the entire field was divided into 33 plots, each with an area of 20 m 2 , with a space of 25 cm between plots and no ridge. All plots were in the same field, had the same growth environment, and had the same soil fertility. Every 3 plots were a growth area (3 repeated experiments), and there were 11 growth areas. Growth areas 1-11 were used for the transplanting of rice seedlings soaked in the seed soaking agents of Examples 1-3 and Comparative Examples 1-8, respectively.

[0073] All growth areas used the same agronomic management measures and water management conditions. After the rice matured, the rice samples were collected, the husked rice portion was removed, it was pulverized, and the cadmium content in the husked rice was determined by the three-acid digestion-graphite furnace atomic absorption method according to the method of “GB5009.15-2014 Food Safety National Standard Determination of Cadmium in Food”, and the cadmium reduction effect was evaluated.

[0074] Other field water and fertilizer management methods and pest control methods are consistent with traditional methods, and after the rice matures (early November), harvest each plot for yield measurement, collect rice grains, and dehull; and determine the cadmium content in rice.

[0075] The determination method of the cadmium content of brown rice is as follows: 0.1 g of brown rice powder sample is taken, 5 mL of nitric acid and 3.0 mL of hydrogen peroxide solution are added, and after being covered, it is cooled and digested overnight, digested for 2 h using a graphite digestion instrument and diluted to 25 mL, and the cadmium concentration in the digestion solution is analyzed and determined using an inductively coupled plasma mass spectrometer. The test results are shown in Table 1:

[0076] Table 1 Difference in cadmium content of brown rice in each growth area

[0077]

[0078] Note 1: In the table, the seed soaking agents are different, and other conventional cultivation methods are consistent.

[0079] Note 2: According to the requirements of GB 2762-2022 National Food Safety Standard Limits of Contaminants in Foods, the cadmium content of qualified rice is less than or equal to 0.2 mg / kg.

[0080] According to the experimental results of the above embodiments, the present application utilizes the synergistic effect of chlorinated hematin, calcium chloride and alpha-ketoglutaric acid. The complex system realizes the source control of cadmium absorption through the triple synergistic effect of chlorinated hematin antioxidant defense, calcium chloride ion antagonism and transport pathway competition, and alpha-ketoglutaric acid metabolic regulation and activation. At the same time, by increasing the solubility of chlorinated hematin in water through sodium dodecyl sulfate SDS, the chlorinated hematin is successfully dispersed and dissolved in water, and the seed germination rate is ensured by the sterilization effect of sodium dodecyl sulfate SDS. When the rice is soaked, the absorption capacity of rice for heavy metal cadmium in the soil can be greatly reduced, and the cadmium reduction efficiency can reach more than 80%. Only the seed soaking agent can reduce the cadmium content in rice to less than or equal to 0.2 mg / kg.

[0081] The application discloses a biochemical compound rice seed soaking agent and application thereof in resisting cadmium in rice brown rice, and belongs to the field of agricultural technology.The components of the biochemical compound rice seed soaking agent are as follows: the concentration of hematin chloride is 10-50 mu mol / L; the concentration of calcium chloride is 1-3 mmol / L; the concentration of alpha-ketoglutaric acid is 0.5-2 mmol / L; the concentration of sodium dodecyl sulfate (SDS) is 5-8 mmol / L; and the solvent is water; the rice seeds are soaked in the compound seed soaking agent for 12-24 hours, then the rice seeds are taken out and naturally dried, and then the rice seeds are sowed in a seedling field after germination reaches the suitable sowing condition; the seedlings are transplanted into a cadmium-polluted field after seedling raising reaches the suitable planting condition; the cadmium accumulation in the cadmium-polluted rice brown rice can be significantly reduced; the synergistic effect of hematin chloride, calcium chloride and alpha-ketoglutaric acid can greatly reduce the absorption capacity of rice to the heavy metal cadmium in the soil during rice seed soaking, and the cadmium reduction efficiency can reach more than 80%.

[0082] At this point, those skilled in the art recognize that, although embodiments of the application have been fully set forth and described herein, many other variations and modifications of the application, in addition to those described and pictured herein, will become apparent to one of ordinary skill in the art upon reading the foregoing description. Accordingly, the scope of the application should be understood to include all such variations and modifications.

Claims

1. A biochemical complex rice seed soaking agent, characterized in that, The biochemical compound rice seed soaking agent is the following components: Medicament and solvent; The medicament is: hematin chloride, calcium chloride, alpha-ketoglutaric acid and sodium dodecyl sulfate; the solvent is water; The biochemical compound rice seed soaking agent component formula is: The concentration of hematin chloride is 10-50 μmol / L; the concentration of calcium chloride is 1-3 mmol / L; the concentration of alpha-ketoglutaric acid is 0.5-2 mmol / L; the concentration of sodium dodecyl sulfate is 5-8 mmol / L; the rest is water; The preparation method of the biochemical compound rice seed soaking agent is as follows: According to the concentration requirement and preparation amount, hematin chloride, calcium chloride, alpha-ketoglutaric acid, sodium dodecyl sulfate and water are weighed; sodium dodecyl sulfate is dissolved in water, then hematin chloride is added, and after uniform stirring, the mixture is left to stand for 10-30 minutes, then calcium chloride and alpha-ketoglutaric acid are added in sequence, and the mixture is stirred and dissolved to obtain the biochemical compound rice seed soaking agent.

2. A biochemical complexed seed soaking agent for rice as claimed in claim 1, wherein, The biochemical compound rice seed soaking agent component formula is: The concentration of hematin chloride is 30 μmol / L; the concentration of calcium chloride is 2 mmol / L; the concentration of alpha-ketoglutaric acid is 1 mmol / L; the concentration of sodium dodecyl sulfate is 6 mmol / L; the rest is water.

3. The method for soaking rice seeds with a biochemical complex rice seed soaking agent according to claim 1, characterized in that, The seed soaking method is specifically: rice seeds are soaked in the biochemical compound rice seed soaking agent; the dosage of the biochemical compound rice seed soaking agent is: the seed soaking agent should completely immerse the rice seeds, and the rice seeds are completely soaked.

4. The rice seed soaking method of biochemical complex rice seed soaking agent as claimed in claim 3, characterized in that, The seed soaking time is 12-24 hours, and the rice variety is one of Ge Liangyou series, Xinganxiachang and Qiliangyou.

5. The method for soaking rice seeds with biochemical complex rice seed soaking agent as claimed in claim 3, characterized in that, The seed soaking temperature is 25-30℃.

6. The application of the biochemical compound rice seed soaking agent in rice brown rice cadmium resistance according to any one of claims 1-2.

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